Lag0010074 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0010074
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	RNase H domain-containing protein
Location	chr9: 44406640 .. 44409858 (-)
RNA-Seq Expression	Lag0010074
Synteny	Lag0010074

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGGAAAAGATTTTGGAAGATCAAATCTATTCCTAAGGCGAAGCTCTGCGCTTGGATAATCATTTACGACTCTATCCCTTCTTCTTCTAATATCAGGAAAAAAGGAATTGATTCTAACTTACCGTGCTCTTTTGCAGGAAATATGAGGAATCAACCATTCACTCCCTTTGGAAATGTAAGTTAGCAAAGAGAATATGGAGACACTTCATCCCTCTAATGAATGTGCTTTTCGGTTTGAACAGGCATTCTTGGAAATCTATCGACTATTGGGCTTGAATTAACGAGAATCTAAAGGAGAAAGAGCTAGAAATCGCCATTCTGATTTTGTGGGAGCTTTGGAACCTCAGAAATAAAGTTATTCACAACGCAGAAATTCCTAATCAGAATTCCATCATCAGATCAGTGGAAGCAAATGTTAAGGAAGGGGAAGCTTACCTCAATCTCGATTCTGCCAGGGAGATTCCAAGATCGAAGAACCAGATGAGTCATGTCCCCTGGTCTTCTCCGCCCCTCGGTTCGTGGACACTCAACGTGGATGCCTCCCGAAATGACTCTAAATTCTTAGGAGGGGTGAGGTGGATTTGGCGTGACTCCTCAGGTTCTCCCATCTGTGAGGGTTGTTCTAGCGTTAAAGGAAGATGGGCTATAAAGATGTTGGAGATGAGAGCGATTCTCGAAGGGTTGCGAAGCCTTCCGACCATCCGGGCTTCGCGCTCAAATCTGACAATCCCGCCTATTGTGGTGATGTCCGACGCTAGTGGCGTGATCAACCTGCTGAACAAAGCAGATTCAGACCATTCGGAAATTTCCTTTATGGTCTCTGAGATTGAAAGCCTGGTTGCTGAGATCGGGTCAATCTCTTTTGTCCATTGCCCGCGTTCGCATAATGAAGATGCTCACAAGCTTGCGCGCCAAGCGGCTTTTGGTTTCTCGGGGGGCTCTAGTTGTTTTTTGGCTTCTTCCAATTCGGAAGAAGGAATTGTTTTGTTGGGCCAACTTGGGCCTGACTTTATTTCTCCACGCTTTTATGGAGTTGTTGTGTAATTATTTTTCCTGCTAATGAAGTTCTGTTTCAAAAAAAAAAAAAAAAATCTTTTTTTTTTCCTCCTAGCATCCTCATGCTCATACTCTCAATCAACACTATCATGTTTCTTCAACAATGGTTCTCCTATAAACATATTTCTCTCACAAATCTCTTTCAACTAATCACCACACTATATTTTCAACTTAATTTTAATGGAGAAGTTAATGAAATGCTTGTTTTGAAAAAATTCAAAAGGCTGATGATTAAAATTTAAATTTTGAAATGATAAGAGTCAAATTGAAATCTAACCTCAAGTTTAGGGTTATAATTGAAACCTACCATATTTATGCTATACAAATCACACTAGATCACTAACACGAGCATAACTCAACTAGCATAAAACCTTATATTAATGACTGTTATAGGTAATTTTGGACCACCATGGCGTACAAGAAGCTGACGAGGACAACTGGGTAGAAAAAGAGCTGAGGAATGGATCCAAGGGGTAAAACCGACAAGTGGGACGGGCCAAGACCGAAGGGGTCGGGCCTTGCCCGACCCCCTGCTCGGCCTCGGCCAAAGGCAGAGGCCGAGGCCATGGTCGGCCGGGCTTGTTCGGTTCCGCTTGGTCCCCACCGCCTCTAGCAGCCTCGGTTCAGCCTGGTTTGTCCCGAAACGCCTCCGAATCCCTAAAGACCCTAGGAGGATGAGCAGGTATTTATATCCCTCTTCGCCACCGAAGAAGGAGACTTCGAATTCTATCCCTAAACACTATTCTATATTTTCTACTCTCTCCTCTTGCTCTTACTTTTCCACTCCCCACCGTTCTGCTTGCTGACTTAAACATCGGAGCCGGTGTGGCGAGCACCACACCGGTGTGCGGGTTTACGGTCTTGCAGGCCACGTCTTCCCCTCTTCTACAAATTTTACCGTTGGTGGCACACGAAGGTCAGGTGAGTTCCTCTGGCCAAATTTGACCATCAACAATGACCAATAAGTTTTTAGTTCGAATCCTCTCACATGTTGTTTAAAAAAAAAAAAAAAACCACCGGGTCTCTTACAAAAAAAAATGCAAAAACGATAGTTTTGCAAAAAATCAAACTTTTTAAAATTTAGAGGAATTACCGAAATCCAACTCGACGAAATTGAGGAAAAGGACGGCAATCTTTTGAGTTTTGATAAAGAACGACACGTGTGATGGATACGTGAGGAGCTCTTTGGAATTTCAATGGGTTTTTTTTAATAATTATTTTAATCAATGGGGTACCGTTTCAAAAATCAAAACTAGCCACGTTAATTTAAATATCACTTTAAATTTATTCATACAACGATGTTTTTGATACACTATTTTTTATTATAATATTTCAACTTACATAAATTATATTGGCAAGCCAAGGTTGACAAGACTACAGAAGTTGTTTGTTATGTTATGTCCAATCTATGCAATTTTGGAGGACATCTCAAAATAGGTTGAATTTGGTGGTGGCATCAAACAAGAACTTAAATGAAAAAACACCGACACATGGACAAAATTACTAACAAAATCGTGGTAAAAATAAGGATTTAGTTGAATGAATGGACCCTAATTTATTGAACTAAAAAGTACATATTTATTTTATCTACCAAAAAAAAAAAAACTTGTTTTGAAAACATTTTCTTGGAAAAAAAAGTACTACTCATGTGCTAGAACCATGAGAGTTTCTCATTTTTTAAAAAGGTTATATGATACATGCACACATTATTATTTAAATTTAATTTTTTAGTTCAACAATATTTGGATATCAGACATTTGAATTCCTAATCAATTGGTCGAGAATACGTATCTATTATCATTAAACTGTCATCACTTTGGCCACGTATTATTTTCTTTAATAAAATGTTGTGCATTGATATATTTTCATTTTTCTATACATATATAATATATTTTTTTGGTCACCAATAAGAGCTTAACTCAACTAACATAAAGTTATACCGATAATCATAAGGTTTGTGGTTTGAATCTTCCCTTCCCCACATATTGTCAAAATAATAATAATAATAATATTGTTGATGGTCGATTTTGGTCAGAGAGAATTCACCTGGCCTTCACGTGCCGCCAACGGTAAATTTGTAGATAAGGGGGGAAGACGTGGCCTGCAAGACGGTAAACCTACACACCGGTGTGGTGCTAGTCACACCGACTCCGATGTTTAA

mRNA sequence

ATGTGGAAAAGATTTTGGAAGATCAAATCTATTCCTAAGGCGAAGCTCTGCGCTTGGATAATCATTTACGACTCTATCCCTTCTTCTTCTAATATCAGGAAAAAAGGAATTGATTCTAACTTACCGTGCTCTTTTGCAGGAAATATGAGGAATCAACCATTCACTCCCTTTGGAAATGAGAAAGAGCTAGAAATCGCCATTCTGATTTTGTGGGAGCTTTGGAACCTCAGAAATAAAGTTATTCACAACGCAGAAATTCCTAATCAGAATTCCATCATCAGATCAGTGGAAGCAAATGTTAAGGAAGGGGAAGCTTACCTCAATCTCGATTCTGCCAGGGAGATTCCAAGATCGAAGAACCAGATGAGTCATGTCCCCTGGTCTTCTCCGCCCCTCGGTTCGTGGACACTCAACGTGGATGCCTCCCGAAATGACTCTAAATTCTTAGGAGGGGTGAGGTGGATTTGGCGTGACTCCTCAGGTTCTCCCATCTGTGAGGGTTGTTCTAGCGTTAAAGGAAGATGGGCTATAAAGATGTTGGAGATGAGAGCGATTCTCGAAGGGTTGCGAAGCCTTCCGACCATCCGGGCTTCGCGCTCAAATCTGACAATCCCGCCTATTGTGGTGATGTCCGACGCTAGTGGCGTGATCAACCTGCTGAACAAAGCAGATTCAGACCATTCGGAAATTTCCTTTATGGTCTCTGAGATTGAAAGCCTGGTTGCTGAGATCGGGTCAATCTCTTTTGTCCATTGCCCGCGTTCGCATAATGAAGATGCTCACAAGCTTGCGCGCCAAGCGGCTTTTGGTTTCTCGGGGGGCTCTAGTTGTTTTTTGGCTTCTTCCAATTCGGAAGAAGGAATTGTTTTGTTGGGCCAACTTGGGCCTGACTTTATTTCTCCACGCTTTTATGGAGTTGTTGTAAAAAGAGCTGAGGAATGGATCCAAGGGGTAAAACCGACAAGTGGGACGGGCCAAGACCGAAGGGGTCGGGCCTTGCCCGACCCCCTGCTCGGCCTCGGCCAAAGGCAGAGGCCGAGGCCATGGTCGGCCGGGCTTGTTCGGTTCCGCTTGGTCCCCACCGCCTCTAGCAGCCTCGGTTCAGCCTGGTTTGTCCCGAAACGCCTCCGAATCCCTAAAGACCCTAGGAGGATGAGCAGGCCACGTCTTCCCCTCTTCTACAAATTTTACCGTTGGTGGCACACGAAGAGAGAATTCACCTGGCCTTCACGTGCCGCCAACGGTAAATTTGTAGATAAGGGGGGAAGACGTGGCCTGCAAGACGGTAAACCTACACACCGGTGTGGTGCTAGTCACACCGACTCCGATGTTTAA

Coding sequence (CDS)

ATGTGGAAAAGATTTTGGAAGATCAAATCTATTCCTAAGGCGAAGCTCTGCGCTTGGATAATCATTTACGACTCTATCCCTTCTTCTTCTAATATCAGGAAAAAAGGAATTGATTCTAACTTACCGTGCTCTTTTGCAGGAAATATGAGGAATCAACCATTCACTCCCTTTGGAAATGAGAAAGAGCTAGAAATCGCCATTCTGATTTTGTGGGAGCTTTGGAACCTCAGAAATAAAGTTATTCACAACGCAGAAATTCCTAATCAGAATTCCATCATCAGATCAGTGGAAGCAAATGTTAAGGAAGGGGAAGCTTACCTCAATCTCGATTCTGCCAGGGAGATTCCAAGATCGAAGAACCAGATGAGTCATGTCCCCTGGTCTTCTCCGCCCCTCGGTTCGTGGACACTCAACGTGGATGCCTCCCGAAATGACTCTAAATTCTTAGGAGGGGTGAGGTGGATTTGGCGTGACTCCTCAGGTTCTCCCATCTGTGAGGGTTGTTCTAGCGTTAAAGGAAGATGGGCTATAAAGATGTTGGAGATGAGAGCGATTCTCGAAGGGTTGCGAAGCCTTCCGACCATCCGGGCTTCGCGCTCAAATCTGACAATCCCGCCTATTGTGGTGATGTCCGACGCTAGTGGCGTGATCAACCTGCTGAACAAAGCAGATTCAGACCATTCGGAAATTTCCTTTATGGTCTCTGAGATTGAAAGCCTGGTTGCTGAGATCGGGTCAATCTCTTTTGTCCATTGCCCGCGTTCGCATAATGAAGATGCTCACAAGCTTGCGCGCCAAGCGGCTTTTGGTTTCTCGGGGGGCTCTAGTTGTTTTTTGGCTTCTTCCAATTCGGAAGAAGGAATTGTTTTGTTGGGCCAACTTGGGCCTGACTTTATTTCTCCACGCTTTTATGGAGTTGTTGTAAAAAGAGCTGAGGAATGGATCCAAGGGGTAAAACCGACAAGTGGGACGGGCCAAGACCGAAGGGGTCGGGCCTTGCCCGACCCCCTGCTCGGCCTCGGCCAAAGGCAGAGGCCGAGGCCATGGTCGGCCGGGCTTGTTCGGTTCCGCTTGGTCCCCACCGCCTCTAGCAGCCTCGGTTCAGCCTGGTTTGTCCCGAAACGCCTCCGAATCCCTAAAGACCCTAGGAGGATGAGCAGGCCACGTCTTCCCCTCTTCTACAAATTTTACCGTTGGTGGCACACGAAGAGAGAATTCACCTGGCCTTCACGTGCCGCCAACGGTAAATTTGTAGATAAGGGGGGAAGACGTGGCCTGCAAGACGGTAAACCTACACACCGGTGTGGTGCTAGTCACACCGACTCCGATGTTTAA

Protein sequence

MWKRFWKIKSIPKAKLCAWIIIYDSIPSSSNIRKKGIDSNLPCSFAGNMRNQPFTPFGNEKELEIAILILWELWNLRNKVIHNAEIPNQNSIIRSVEANVKEGEAYLNLDSAREIPRSKNQMSHVPWSSPPLGSWTLNVDASRNDSKFLGGVRWIWRDSSGSPICEGCSSVKGRWAIKMLEMRAILEGLRSLPTIRASRSNLTIPPIVVMSDASGVINLLNKADSDHSEISFMVSEIESLVAEIGSISFVHCPRSHNEDAHKLARQAAFGFSGGSSCFLASSNSEEGIVLLGQLGPDFISPRFYGVVVKRAEEWIQGVKPTSGTGQDRRGRALPDPLLGLGQRQRPRPWSAGLVRFRLVPTASSSLGSAWFVPKRLRIPKDPRRMSRPRLPLFYKFYRWWHTKREFTWPSRAANGKFVDKGGRRGLQDGKPTHRCGASHTDSDV

Homology

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BLAST of Lag0010074 vs. NCBI nr
Match: XP_022155262.1 (uncharacterized protein LOC111022403 [Momordica charantia])

HSP 1 Score: 103.6 bits (257), Expect = 4.5e-18
Identity = 66/210 (31.43%), Postives = 114/210 (54.29%), Query Frame = 0


Query: 59  NEKELEIAILILWELWNLRNKVIHNAEIPNQNSIIRSVEANVKEGEAYLNLDSAREIPRS 118
           ++++L++ ++  W +WN RN VI   E  + +++I+ +   V E  +Y +  S   + ++
Sbjct: 6   SDEDLDVLLIGSWVIWNHRNYVIFRGEHSSFSTMIQQLTKFVTE-SSYQSETSLSMLHKT 65

Query: 119 KNQMSHVPWSSPPLGSWTLNVDASRNDSKFLGGVRWIWRDSSGSPICEGCSSVKGRWAIK 178
            N  + + W  PP+  WTLN DAS +DS   GG+ WI R   G  +  G   V+    +K
Sbjct: 66  LN--NKLKWEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVK 125

Query: 179 MLEMRAILEGLRSLPTIRASRSNLTIPPIVVMSDASGVINLLNKADSDHSEISFMVSEIE 238
           +LE  AILEGLR+L  +   R      P+ + +D++ V +LLN+   D ++  ++V EI 
Sbjct: 126 LLEASAILEGLRNLTNLGVLR------PLHIETDSAEVESLLNRKREDLTKTGWVVEEIL 185

Query: 239 SLVAEIGSISFVHCPRSHNEDAHKLARQAA 269
           +L      ++F    R  N  AH LA++A+
Sbjct: 186 NLRDSCEILAFAKVGRETNGCAHSLAQRAS 206

[Close]

BLAST of Lag0010074 vs. NCBI nr
Match: KAF4360260.1 (hypothetical protein F8388_020551 [Cannabis sativa] >KAF4393916.1 hypothetical protein G4B88_025885 [Cannabis sativa])

HSP 1 Score: 97.4 bits (241), Expect = 3.2e-16
Identity = 71/302 (23.51%), Postives = 124/302 (41.06%), Query Frame = 0


Query: 2   WKRFWKIKSIPKAKLCAWIIIYDSIPSSSNIRKKGIDSNLPCSFAGNM------------ 61
           WK  W ++  P+ KL  W +  + +PS +N+  +G+  +  C   GN             
Sbjct: 68  WKMMWSMQLPPRMKLFGWRVCQNWLPSKTNLSHRGMTIHPSCDLCGNQAETLTHALWSCS 127

Query: 62  ------------RNQPFTPFG-------------NEKELEIAILILWELWNLRNKVIHNA 121
                       ++  +   G             N  E E AI I+W +W  RN+  +N 
Sbjct: 128 KIKPIWKLVPWYKHCEYLNQGSMFDILVSLKAKLNMNEFEDAIKIMWAIWENRNRKWNNL 187

Query: 122 EIPNQNSIIRSVEANVKEGEAYLNLDSAREIPRSKNQMSHVPWSSPPLGSWTLNVDASRN 181
            + N   ++  V +        +  D    +  SK       W  PP G   +N DA+ N
Sbjct: 188 HVMNGAQLLDWVFSAYPGSSTPVKNDMTTNMELSKT----THWIRPPAGYIAVNCDAAMN 247

Query: 182 DSKFLGGVRWIWRDSSGSPICEGCSSVKGRWAIKMLEMRAILEGLRSLPTIRASRSNLTI 241
                 G  +IWR+  G  +  G +      ++ M E  AILE L ++P       N T 
Sbjct: 248 SGAAGIGTGFIWRNWDGKLLIAGMNYSNRCCSVDMAEAWAILEALNNMP-------NTTD 307

Query: 242 PPIVVMSDASGVINLLNKADSDHSEISFMVSEIESLVAEIGSISFVHCPRSHNEDAHKLA 267
            P+ + SD   +++ +   D+  + ++ ++ +I   + ++   S VH  R++NE AH LA
Sbjct: 308 SPMEIQSDCKHLVDKIMNQDNHLTAVNTIIHQIRMQMEKLNCNSIVHVNRNNNECAHMLA 358

[Close]

BLAST of Lag0010074 vs. NCBI nr
Match: XP_022143535.1 (uncharacterized protein LOC111013412 [Momordica charantia])

HSP 1 Score: 94.7 bits (234), Expect = 2.1e-15
Identity = 65/218 (29.82%), Postives = 101/218 (46.33%), Query Frame = 0


Query: 60  EKELEIAILILWELWNLRNKVIHNAEIPNQNSIIRSVE----------ANVKEGEAYLNL 119
           E+E   +++I W++W +RNK I     P    I  +++           N+K      +L
Sbjct: 6   EEERRRSMIIAWQIWEMRNKSIFKGVHPETRDIQLAIDRYIINSAGRNTNLKGKSTNKDL 65

Query: 120 DSAREIPRSKNQMSHVPWSSPPLGSWTLNVDASRNDSKFLGGVRWIWRDSSGSPICEGCS 179
              R I       +   W  P   SW LN +A+       GG+ WI RD  G  I   C 
Sbjct: 66  HLIRRI----EDNTGAQWKPPTSNSWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCR 125

Query: 180 SVKGRWAIKMLEMRAILEGLRSLPTIRASRSNLTIPPIVVMSDASGVINLLNKADSDHSE 239
            ++    I  LE+ AI EGLR+   IR         PI + SD+   I+LL++   D +E
Sbjct: 126 IIRAERNITYLEVMAICEGLRA---IRQEHCR----PIHLESDSLEAIHLLHRQCQDQTE 185

Query: 240 ISFMVSEIESLVAEIGSISFVHCPRSHNEDAHKLARQA 268
           I +++ EI  ++ ++  +S  H  R  N+ AH LAR+A
Sbjct: 186 IIWLLEEIWQMMKDMEIVSMRHISREANKVAHGLARRA 212

[Close]

BLAST of Lag0010074 vs. NCBI nr
Match: EEE69144.1 (hypothetical protein OsJ_28268 [Oryza sativa Japonica Group])

HSP 1 Score: 94.7 bits (234), Expect = 2.1e-15
Identity = 77/316 (24.37%), Postives = 139/316 (43.99%), Query Frame = 0


Query: 6    WKIKSIPKAKLCAWIIIYDSIPSSSNIRKKGIDSNLPCSFAG------------------ 65
            WK  +  K K+ AW  I +S+ +  N +K+ ++ +  C+  G                  
Sbjct: 921  WKCNAPQKVKIFAWRAITNSLTTLENKKKRKLEVSDICTICGVESEYVVHALFHCPHARQ 980

Query: 66   -------NMRNQPFTPFGN--------------EKELEIAILILWELWNLRNKVIHNAEI 125
                   +M+  P +   N               ++  + +++LW +W+ RN+++H    
Sbjct: 981  LWEAMSDDMQLNPLSNIHNGDSKVILDLLEQSQPEDQVMLLMVLWRIWHTRNEIVHGKPA 1040

Query: 126  P--------------NQNSIIRSVEANVKEGEAYLN--LDSAREIPRSKNQMSHVPWSSP 185
            P              +   I +  +AN ++G+  ++  L  +  I RS+ + +   WS P
Sbjct: 1041 PGILVSKRFIESYVLSLAEIKQHPQANPEKGKHVVDVVLKKSHSIKRSR-EPAPDKWSKP 1100

Query: 186  PLGSWTLNVDASRNDSKFLGGVRWIWRDSSGSPICEGCSSVKGRWAIKMLEMRAILEGLR 245
              GS  LNVD S  +S+  GG+  + R+ +G  I   C  V    +   +E+ A  +GL 
Sbjct: 1101 LPGSMKLNVDGSFQESEGKGGIGAVLRNCTGEVIFAACGHVDHCSSALEMELLASRDGL- 1160

Query: 246  SLPTIRASRSNLTIPPIVVMSDASGVINLLNKADSDHSEISFMVSEIESLVAEIGSISFV 267
                  A     T+ PIV+ +D   +++L   A    SE++F+++EI+SL+     ISF 
Sbjct: 1161 ------ALALQWTLLPIVIETDCLAMVHLFRDATGAKSELAFLITEIDSLLVGNRDISFN 1220

[Close]

BLAST of Lag0010074 vs. NCBI nr
Match: XP_015382610.1 (uncharacterized protein LOC107175578 [Citrus sinensis])

HSP 1 Score: 94.4 bits (233), Expect = 2.7e-15
Identity = 58/208 (27.88%), Postives = 100/208 (48.08%), Query Frame = 0


Query: 59  NEKELEIAILILWELWNLRNKVIHNAEIPNQNSIIRSVEANVKEGEAYLNLDSAREIPRS 118
           N+ +LE+ I+I W +WN RN+++   +  N  +++   EA +   EAY  +    ++ + 
Sbjct: 20  NKADLELMIMICWMVWNARNEMLFKGKRENPQALVAKAEAVL---EAYKRVQKPEQVRKE 79

Query: 119 KNQM-SHVPWSSPPLGSWTLNVDASRNDSKFLGGVRWIWRDSSGSPICEGCSSVKGRWAI 178
             Q+ +   W+ P  G   +NVDA+ +  K L G+  I RD  G+ I       K    +
Sbjct: 80  SQQVQTQQVWNPPQNGFVKVNVDAATSSEKHLAGLGAIIRDDQGTVIAAAIKISKFNGDV 139

Query: 179 KMLEMRAILEGLRSLPTIRASRSNLTIPPIVVMSDASGVINLLNKADSDHSEISFMVSEI 238
              E   +  GL+          N +   ++V SDA  V+ L+N      SEI +++SEI
Sbjct: 140 SFAEAETLEWGLQ-------IARNASAKALIVESDAQSVVKLVNNKQGGKSEIFWIISEI 199

Query: 239 ESLVAEIGSISFVHCPRSHNEDAHKLAR 266
           ++L+     +S  +  RS N   H LA+
Sbjct: 200 QNLIRNFELVSINYTHRSRNAITHSLAK 217

[Close]

BLAST of Lag0010074 vs. ExPASy TrEMBL
Match: A0A7C8ZRF8 (Uncharacterized protein (Fragment) OS=Opuntia streptacantha OX=393608 PE=4 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 2.6e-19
Identity = 74/273 (27.11%), Postives = 126/273 (46.15%), Query Frame = 0


Query: 2   WKRFWKIKSIPKAKLCAWIIIYDSIPSSSNIRKKGIDSNLPCSFAGNMRNQPFTPFGNEK 61
           W + W+ K+ P+ K  AW    D++P+  N+ +KG+  ++ C            PF +++
Sbjct: 17  WNKIWRSKTQPRIKHFAWRACQDTLPTRVNLFRKGVKVDVYC------------PFCDDE 76

Query: 62  ELEIAILILWELWNLRNKVIHNAEIPNQNSII-------RSVEANVKEGEAYLNLDSARE 121
              + +  LW +WN RNK I   +  N  ++I       R +   +K+ E  L L S   
Sbjct: 77  TRGLFLTFLWVIWNSRNKWIFEEKRWNTLTMINYAVDYWRILLEAIKQKEGCLRLASE-- 136

Query: 122 IPRSKNQMSHVPWSSPPLGSWTLNVDASRNDSKFLGGVRWIWRDSSGSPICEGCSSVKGR 181
                   ++V W  PP G   +N DAS    +F  G+  + RD  G  +   C+ + G 
Sbjct: 137 --------ANVIWEPPPAGVVKINTDASLASGEF-RGLGAVLRDHLGGLVASACAKMVGN 196

Query: 182 WAIKMLEMRAILEGLRSLPTIRASRSNLTIPPIVVMSDASGVINLLNKADSDHSEISFMV 241
           W  +  E+ A+  G++          +L +  I++ +DA+GV + LN         S  V
Sbjct: 197 WDAESSEVCAVRFGMK-------VAKSLGLNHIILETDATGVAHALNSKTYKLDYASTFV 256

Query: 242 SEIESLVAEIGSISFVHCPRSHNEDAHKLARQA 268
            +   L+ +  S+ F+H  RS N+ AH+LAR A
Sbjct: 257 KDCHHLINDFSSVKFMHAKRSCNKVAHELARVA 259

[Close]

BLAST of Lag0010074 vs. ExPASy TrEMBL
Match: A0A6J1DNV9 (uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022403 PE=4 SV=1)

HSP 1 Score: 103.6 bits (257), Expect = 2.2e-18
Identity = 66/210 (31.43%), Postives = 114/210 (54.29%), Query Frame = 0


Query: 59  NEKELEIAILILWELWNLRNKVIHNAEIPNQNSIIRSVEANVKEGEAYLNLDSAREIPRS 118
           ++++L++ ++  W +WN RN VI   E  + +++I+ +   V E  +Y +  S   + ++
Sbjct: 6   SDEDLDVLLIGSWVIWNHRNYVIFRGEHSSFSTMIQQLTKFVTE-SSYQSETSLSMLHKT 65

Query: 119 KNQMSHVPWSSPPLGSWTLNVDASRNDSKFLGGVRWIWRDSSGSPICEGCSSVKGRWAIK 178
            N  + + W  PP+  WTLN DAS +DS   GG+ WI R   G  +  G   V+    +K
Sbjct: 66  LN--NKLKWEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVK 125

Query: 179 MLEMRAILEGLRSLPTIRASRSNLTIPPIVVMSDASGVINLLNKADSDHSEISFMVSEIE 238
           +LE  AILEGLR+L  +   R      P+ + +D++ V +LLN+   D ++  ++V EI 
Sbjct: 126 LLEASAILEGLRNLTNLGVLR------PLHIETDSAEVESLLNRKREDLTKTGWVVEEIL 185

Query: 239 SLVAEIGSISFVHCPRSHNEDAHKLARQAA 269
           +L      ++F    R  N  AH LA++A+
Sbjct: 186 NLRDSCEILAFAKVGRETNGCAHSLAQRAS 206

[Close]

BLAST of Lag0010074 vs. ExPASy TrEMBL
Match: A0A6J1CP26 (uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013412 PE=4 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 1.0e-15
Identity = 65/218 (29.82%), Postives = 101/218 (46.33%), Query Frame = 0


Query: 60  EKELEIAILILWELWNLRNKVIHNAEIPNQNSIIRSVE----------ANVKEGEAYLNL 119
           E+E   +++I W++W +RNK I     P    I  +++           N+K      +L
Sbjct: 6   EEERRRSMIIAWQIWEMRNKSIFKGVHPETRDIQLAIDRYIINSAGRNTNLKGKSTNKDL 65

Query: 120 DSAREIPRSKNQMSHVPWSSPPLGSWTLNVDASRNDSKFLGGVRWIWRDSSGSPICEGCS 179
              R I       +   W  P   SW LN +A+       GG+ WI RD  G  I   C 
Sbjct: 66  HLIRRI----EDNTGAQWKPPTSNSWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCR 125

Query: 180 SVKGRWAIKMLEMRAILEGLRSLPTIRASRSNLTIPPIVVMSDASGVINLLNKADSDHSE 239
            ++    I  LE+ AI EGLR+   IR         PI + SD+   I+LL++   D +E
Sbjct: 126 IIRAERNITYLEVMAICEGLRA---IRQEHCR----PIHLESDSLEAIHLLHRQCQDQTE 185

Query: 240 ISFMVSEIESLVAEIGSISFVHCPRSHNEDAHKLARQA 268
           I +++ EI  ++ ++  +S  H  R  N+ AH LAR+A
Sbjct: 186 IIWLLEEIWQMMKDMEIVSMRHISREANKVAHGLARRA 212

[Close]

BLAST of Lag0010074 vs. ExPASy TrEMBL
Match: B9FYJ3 (Uncharacterized protein OS=Oryza sativa subsp. japonica OX=39947 GN=OsJ_28268 PE=3 SV=1)

HSP 1 Score: 94.7 bits (234), Expect = 1.0e-15
Identity = 77/316 (24.37%), Postives = 139/316 (43.99%), Query Frame = 0


Query: 6    WKIKSIPKAKLCAWIIIYDSIPSSSNIRKKGIDSNLPCSFAG------------------ 65
            WK  +  K K+ AW  I +S+ +  N +K+ ++ +  C+  G                  
Sbjct: 921  WKCNAPQKVKIFAWRAITNSLTTLENKKKRKLEVSDICTICGVESEYVVHALFHCPHARQ 980

Query: 66   -------NMRNQPFTPFGN--------------EKELEIAILILWELWNLRNKVIHNAEI 125
                   +M+  P +   N               ++  + +++LW +W+ RN+++H    
Sbjct: 981  LWEAMSDDMQLNPLSNIHNGDSKVILDLLEQSQPEDQVMLLMVLWRIWHTRNEIVHGKPA 1040

Query: 126  P--------------NQNSIIRSVEANVKEGEAYLN--LDSAREIPRSKNQMSHVPWSSP 185
            P              +   I +  +AN ++G+  ++  L  +  I RS+ + +   WS P
Sbjct: 1041 PGILVSKRFIESYVLSLAEIKQHPQANPEKGKHVVDVVLKKSHSIKRSR-EPAPDKWSKP 1100

Query: 186  PLGSWTLNVDASRNDSKFLGGVRWIWRDSSGSPICEGCSSVKGRWAIKMLEMRAILEGLR 245
              GS  LNVD S  +S+  GG+  + R+ +G  I   C  V    +   +E+ A  +GL 
Sbjct: 1101 LPGSMKLNVDGSFQESEGKGGIGAVLRNCTGEVIFAACGHVDHCSSALEMELLASRDGL- 1160

Query: 246  SLPTIRASRSNLTIPPIVVMSDASGVINLLNKADSDHSEISFMVSEIESLVAEIGSISFV 267
                  A     T+ PIV+ +D   +++L   A    SE++F+++EI+SL+     ISF 
Sbjct: 1161 ------ALALQWTLLPIVIETDCLAMVHLFRDATGAKSELAFLITEIDSLLVGNRDISFN 1220

[Close]

BLAST of Lag0010074 vs. ExPASy TrEMBL
Match: A0A6J1DSV1 (uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023608 PE=4 SV=1)

HSP 1 Score: 92.4 bits (228), Expect = 5.0e-15
Identity = 66/219 (30.14%), Postives = 103/219 (47.03%), Query Frame = 0


Query: 60  EKELEIAILILWELWNLRNKVI--------HNAEIPNQNSIIRSV--EANVKEGEAYLNL 119
           E+E   +++I W++W +RNK I         + ++     II S   + N+K   A  +L
Sbjct: 6   EEERRRSMIIAWQIWEMRNKSIFKGVHSETRDIQLVIDRYIINSAGRDTNLKGKSANKDL 65

Query: 120 DSAREIPRSKNQMSHVPWSSPPLGSWTLNVDASRNDSKFLGGVRWIWRDSSGSPICEGCS 179
              R I       +   W  P   SW LN DA+       GG+ WI RD  G  I   C 
Sbjct: 66  HLIRRI----GDNTGARWKPPTSNSWKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCR 125

Query: 180 SVKGRWAIKMLEMRAILEGLRSLPTIRASR-SNLTIPPIVVMSDASGVINLLNKADSDHS 239
            ++    I  LE+ AI EGLR++              PI + SD+   I+LL++   D +
Sbjct: 126 IIRTERNITYLEVMAICEGLRAIRQEHCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQT 185

Query: 240 EISFMVSEIESLVAEIGSISFVHCPRSHNEDAHKLARQA 268
           EI +++ EI  ++ ++  +S  H  R  N+ AH LAR+A
Sbjct: 186 EIIWLLEEIWQMMEDMKIVSMRHISREANKVAHDLARRA 220

[Close]

BLAST of Lag0010074 vs. TAIR 10
Match: AT5G65005.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 51.6 bits (122), Expect = 1.9e-06
Identity = 40/199 (20.10%), Postives = 78/199 (39.20%), Query Frame = 0


Query: 69  ILWELWNLRNKVIHNAEIPNQNSIIRSVEANVKEGEAYLNLDSAREIPRSKNQMSHVPWS 128
           ++W +W   N ++ N       + +     + KE       +  +   R+ +   +  WS
Sbjct: 47  LMWRIWKSGNDLVFNHTRTKFQTTVEMALNDTKEWLDNTMTNEQQNGNRNADPSRNTKWS 106

Query: 129 SPPLGSWTLNVDASRNDSKFLGGVRWIWRDSSGSPICEGCSSVKGRWAIKMLEMRAILEG 188
            P       N DAS ++   + G+ WI R+S G+ I  G    +GR   +  E   ++  
Sbjct: 107 PPGRDKLKCNYDASHHERNTVSGLGWILRNSQGTVIECGMGKFQGRMTTEEAECSTLIWA 166

Query: 189 LRSLPTIRASRSNLTIPPIVVMSDASGVINLLNKADSDHSEISFMVSEIESLVAEIGSIS 248
           +++       +       ++   D   +  ++N   S +  +   +  I+S +    SI 
Sbjct: 167 IQASYGFGHKK-------VIFEGDNQTITRMIN-TKSSNPRLQHFLDTIQSWIPSFESIE 226

Query: 249 FVHCPRSHNEDAHKLARQA 268
           F    R  N  A  LA+QA
Sbjct: 227 FSFKHREQNGCADFLAKQA 237

[Close]

BLAST of Lag0010074 vs. TAIR 10
Match: AT1G52990.1 (thioredoxin family protein )

HSP 1 Score: 47.4 bits (111), Expect = 3.5e-05
Identity = 34/145 (23.45%), Postives = 65/145 (44.83%), Query Frame = 0


Query: 123 SHVPWSSPPLGSWTLNVDASRNDSKFLGGVRWIWRDSSGSPICEGCSSVKGRWAIKMLEM 182
           ++V  SS P      N DAS ++   + G+ W+ R+S G+ +  G    +GR   +  E 
Sbjct: 36  TYVSSSSVPSCRVKCNYDASHHEGDVVSGLGWLIRNSQGTVLECGMGKFQGRMTPEEAEC 95

Query: 183 RAILEGLRSLPTIRASRSNLTIPPIVVMSDASGVINLLNKADSDHSEISFMVSEIESLVA 242
            A++  +++      ++       ++   D S V  L+N   SD+  +   +  I+S + 
Sbjct: 96  SALIWAIQATSAFGYTK-------VIFEGDNSNVNRLIN-TKSDNPRLKHYLDTIKSWIP 155

Query: 243 EIGSISFVHCPRSHNEDAHKLARQA 268
              S  F+   R  N+ A  L ++A
Sbjct: 156 SFTSTEFIFTHREQNQCADTLVKKA 172

The following BLAST results are available for this feature:

BLAST of Lag0010074 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022155262.1	4.5e-18	31.43	uncharacterized protein LOC111022403 [Momordica charantia]	[more]
KAF4360260.1	3.2e-16	23.51	hypothetical protein F8388_020551 [Cannabis sativa] >KAF4393916.1 hypothetical p...	[more]
XP_022143535.1	2.1e-15	29.82	uncharacterized protein LOC111013412 [Momordica charantia]	[more]
EEE69144.1	2.1e-15	24.37	hypothetical protein OsJ_28268 [Oryza sativa Japonica Group]	[more]
XP_015382610.1	2.7e-15	27.88	uncharacterized protein LOC107175578 [Citrus sinensis]	[more]

BLAST of Lag0010074 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Lag0010074 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A7C8ZRF8	2.6e-19	27.11	Uncharacterized protein (Fragment) OS=Opuntia streptacantha OX=393608 PE=4 SV=1	[more]
A0A6J1DNV9	2.2e-18	31.43	uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022...	[more]
A0A6J1CP26	1.0e-15	29.82	uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013...	[more]
B9FYJ3	1.0e-15	24.37	Uncharacterized protein OS=Oryza sativa subsp. japonica OX=39947 GN=OsJ_28268 PE...	[more]
A0A6J1DSV1	5.0e-15	30.14	uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023...	[more]

BLAST of Lag0010074 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G65005.1	1.9e-06	20.10	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT1G52990.1	3.5e-05	23.45	thioredoxin family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 135..270 e-value: 1.3E-14 score: 56.2
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 138..267 e-value: 9.4E-17 score: 61.0
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 417..444
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 319..339
None	No IPR available	PANTHER	PTHR33033	POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATED	coord: 124..267
None	No IPR available	PANTHER	PTHR33033:SF67	SUBFAMILY NOT NAMED	coord: 124..267
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 137..265 e-value: 2.19144E-19 score: 81.5916
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 134..269

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0010074.1	Lag0010074.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity