Lag0010034 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0010034
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	NEDD8-specific protease 1
Location	chr9: 44135129 .. 44135836 (+)
RNA-Seq Expression	Lag0010034
Synteny	Lag0010034

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGGAAATCCGAAGGAGATAAGAAGATCTTAAGCTACAACGATGTTGTACTCAGAAAATCAGACTTAGACATCCTCGGTGGTCCTTATTATCTAAATGATCGAATCATTGAGTTTTATTTTAGCTATTTATGCACACGCCATCCTTCTAAGGACATTCTTCTAGCTCCACCTTCAATTGCTTTCTGGATAATGAACTGCCCCGACCCCGAAAGTCTCAACGATTTCCTAGAACCTCTCAATCTACGCGACAAGAAGCTGGTAATCTTCCCGGTCAACGACAACAATGATGTTAGTAAGGCCGAAGGTGGAAACCACTGGAGTCTTCTTGCTTTTTACAGAGAAGCGAACGTATTTGTTCATCACGATAGCAACAAAGGAATGAACAAATACCACGCCAAGCGACTCTACAAAGCCGTGGCTAGATTTACCGGTGGCTCGAATTCGACATCTGATCCCAGTTATGTGGAATGTAGTGAGTCTCCTCAGCAAGTAAATGGGCATGATTGTGGTTTATATGTTACAGCTATAGCAAGAACTATATGCAGCTGGTTTGAGCAGAGGAAAGATTTTCATGGAGATGGTTTGTGGTTTTCTGCAGTGGTGGAGGAGATTAGTCCATCTTTGGTTGCTGATATGCGTAATGAAATCTTGGGGCTAATTAAAAGTTTAATGGCTTTGAAGGAATACCAACCAGCTTCTAAATAA

mRNA sequence

ATGTGGAAATCCGAAGGAGATAAGAAGATCTTAAGCTACAACGATGTTGTACTCAGAAAATCAGACTTAGACATCCTCGGTGGTCCTTATTATCTAAATGATCGAATCATTGAGTTTTATTTTAGCTATTTATGCACACGCCATCCTTCTAAGGACATTCTTCTAGCTCCACCTTCAATTGCTTTCTGGATAATGAACTGCCCCGACCCCGAAAGTCTCAACGATTTCCTAGAACCTCTCAATCTACGCGACAAGAAGCTGGTAATCTTCCCGGTCAACGACAACAATGATGTTAGTAAGGCCGAAGGTGGAAACCACTGGAGTCTTCTTGCTTTTTACAGAGAAGCGAACGTATTTGTTCATCACGATAGCAACAAAGGAATGAACAAATACCACGCCAAGCGACTCTACAAAGCCGTGGCTAGATTTACCGGTGGCTCGAATTCGACATCTGATCCCAGTTATGTGGAATGTAGTGAGTCTCCTCAGCAAGTAAATGGGCATGATTGTGGTTTATATGTTACAGCTATAGCAAGAACTATATGCAGCTGGTTTGAGCAGAGGAAAGATTTTCATGGAGATGGTTTGTGGTTTTCTGCAGTGGTGGAGGAGATTAGTCCATCTTTGGTTGCTGATATGCGTAATGAAATCTTGGGGCTAATTAAAAGTTTAATGGCTTTGAAGGAATACCAACCAGCTTCTAAATAA

Coding sequence (CDS)

ATGTGGAAATCCGAAGGAGATAAGAAGATCTTAAGCTACAACGATGTTGTACTCAGAAAATCAGACTTAGACATCCTCGGTGGTCCTTATTATCTAAATGATCGAATCATTGAGTTTTATTTTAGCTATTTATGCACACGCCATCCTTCTAAGGACATTCTTCTAGCTCCACCTTCAATTGCTTTCTGGATAATGAACTGCCCCGACCCCGAAAGTCTCAACGATTTCCTAGAACCTCTCAATCTACGCGACAAGAAGCTGGTAATCTTCCCGGTCAACGACAACAATGATGTTAGTAAGGCCGAAGGTGGAAACCACTGGAGTCTTCTTGCTTTTTACAGAGAAGCGAACGTATTTGTTCATCACGATAGCAACAAAGGAATGAACAAATACCACGCCAAGCGACTCTACAAAGCCGTGGCTAGATTTACCGGTGGCTCGAATTCGACATCTGATCCCAGTTATGTGGAATGTAGTGAGTCTCCTCAGCAAGTAAATGGGCATGATTGTGGTTTATATGTTACAGCTATAGCAAGAACTATATGCAGCTGGTTTGAGCAGAGGAAAGATTTTCATGGAGATGGTTTGTGGTTTTCTGCAGTGGTGGAGGAGATTAGTCCATCTTTGGTTGCTGATATGCGTAATGAAATCTTGGGGCTAATTAAAAGTTTAATGGCTTTGAAGGAATACCAACCAGCTTCTAAATAA

Protein sequence

MWKSEGDKKILSYNDVVLRKSDLDILGGPYYLNDRIIEFYFSYLCTRHPSKDILLAPPSIAFWIMNCPDPESLNDFLEPLNLRDKKLVIFPVNDNNDVSKAEGGNHWSLLAFYREANVFVHHDSNKGMNKYHAKRLYKAVARFTGGSNSTSDPSYVECSESPQQVNGHDCGLYVTAIARTICSWFEQRKDFHGDGLWFSAVVEEISPSLVADMRNEILGLIKSLMALKEYQPASK

Homology

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BLAST of Lag0010034 vs. NCBI nr
Match: XP_038902669.1 (NEDD8-specific protease 1 [Benincasa hispida])

HSP 1 Score: 421.0 bits (1081), Expect = 6.7e-114
Identity = 200/236 (84.75%), Postives = 219/236 (92.80%), Query Frame = 0


Query: 1   MWKSEGDKKILSYNDVVLRKSDLDILGGPYYLNDRIIEFYFSYLCTRHPSKDILLAPPSI 60
           M KSEGD+KILSYNDVVLRKSDLDILGGPYYLNDRI EFYFSYL T HPSKDILL+PPSI
Sbjct: 1   MGKSEGDEKILSYNDVVLRKSDLDILGGPYYLNDRIPEFYFSYLSTTHPSKDILLSPPSI 60

Query: 61  AFWIMNCPDPESLNDFLEPLNLRDKKLVIFPVNDNNDVSKAEGGNHWSLLAFYREANVFV 120
           AFWIMNCPD ESL+DFL+PLNL DKKLVIFPVNDNNDVSKAEGGNHWSLLAFYREANVFV
Sbjct: 61  AFWIMNCPDAESLDDFLKPLNLCDKKLVIFPVNDNNDVSKAEGGNHWSLLAFYREANVFV 120

Query: 121 HHDSNKGMNKYHAKRLYKAVARF-TGGSNSTSDPSYVECSESPQQVNGHDCGLYVTAIAR 180
           HHDS+KGMNKYHAKRLY+AVARF +  SNSTS+PSY+EC ESPQQVNG+DCG+YV AIAR
Sbjct: 121 HHDSHKGMNKYHAKRLYRAVARFMSADSNSTSNPSYLECVESPQQVNGYDCGVYVIAIAR 180

Query: 181 TICSWFEQRKDFHGDGLWFSAVVEEISPSLVADMRNEILGLIKSLMALKEYQPASK 236
           TIC W+EQRK+   DGLWFSAVV+E+SPS V +MR+EILGLI+SLMA+KEYQPASK
Sbjct: 181 TICRWYEQRKEVDRDGLWFSAVVKEVSPSFVTNMRSEILGLIRSLMAVKEYQPASK 236

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BLAST of Lag0010034 vs. NCBI nr
Match: XP_008463889.1 (PREDICTED: NEDD8-specific protease 1 [Cucumis melo] >KAA0035294.1 NEDD8-specific protease 1 [Cucumis melo var. makuwa] >TYK14342.1 NEDD8-specific protease 1 [Cucumis melo var. makuwa])

HSP 1 Score: 407.9 bits (1047), Expect = 5.9e-110
Identity = 194/236 (82.20%), Postives = 214/236 (90.68%), Query Frame = 0


Query: 1   MWKSEGDKKILSYNDVVLRKSDLDILGGPYYLNDRIIEFYFSYLCTRHPSKDILLAPPSI 60
           M K EGD+KILS+NDVVLRKSDL+IL GPY+LNDRIIEFYFSYL T HPS DILL+PPSI
Sbjct: 1   MGKFEGDEKILSFNDVVLRKSDLEILSGPYFLNDRIIEFYFSYLSTTHPSNDILLSPPSI 60

Query: 61  AFWIMNCPDPESLNDFLEPLNLRDKKLVIFPVNDNNDVSKAEGGNHWSLLAFYREANVFV 120
           AFWIMNCPD ESLN FLEPLNLR KKLVIFPVNDN+DVSK EGGNHWSLLAFYREAN+FV
Sbjct: 61  AFWIMNCPDVESLNGFLEPLNLRHKKLVIFPVNDNSDVSKPEGGNHWSLLAFYREANIFV 120

Query: 121 HHDSNKGMNKYHAKRLYKAVARF-TGGSNSTSDPSYVECSESPQQVNGHDCGLYVTAIAR 180
           HHDSNKGMNKY AKRLYKAVARF   GSNSTS+PSY+EC ESPQQVNG+DCG+YVTAIAR
Sbjct: 121 HHDSNKGMNKYDAKRLYKAVARFMNDGSNSTSNPSYMECVESPQQVNGYDCGVYVTAIAR 180

Query: 181 TICSWFEQRKDFHGDGLWFSAVVEEISPSLVADMRNEILGLIKSLMALKEYQPASK 236
           +IC W+E+R++   DGLWFSAVVEEISP LVA+MR EIL LI+SLMA+KEYQPA+K
Sbjct: 181 SICKWYEERREVEKDGLWFSAVVEEISPLLVANMRREILELIRSLMAVKEYQPATK 236

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BLAST of Lag0010034 vs. NCBI nr
Match: XP_022146090.1 (NEDD8-specific protease 1 [Momordica charantia])

HSP 1 Score: 403.3 bits (1035), Expect = 1.4e-108
Identity = 191/236 (80.93%), Postives = 212/236 (89.83%), Query Frame = 0


Query: 1   MWKSEGDKKILSYNDVVLRKSDLDILGGPYYLNDRIIEFYFSYLCTRHPSKDILLAPPSI 60
           M KSEGDKKILSYNDVVLRKSDLDIL GPYYLNDRIIEFYFSYL + HPSKDILLAPPSI
Sbjct: 1   MRKSEGDKKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLSSYHPSKDILLAPPSI 60

Query: 61  AFWIMNCPDPESLNDFLEPLNLRDKKLVIFPVNDNNDVSKAEGGNHWSLLAFYREANVFV 120
           AFW MNCPD  SL+DFL PL+L +KKLVIFPVNDN+DVSKAEGGNHWSLLAFYRE+N F+
Sbjct: 61  AFWTMNCPDSNSLDDFLRPLDLFNKKLVIFPVNDNDDVSKAEGGNHWSLLAFYRESNTFI 120

Query: 121 HHDSNKGMNKYHAKRLYKAVARFTGG-SNSTSDPSYVECSESPQQVNGHDCGLYVTAIAR 180
           HHDSN+GMNK+HAKRLYKAVARFT   SN TS+P YVEC ESPQQVNG+DCGLYVTAIAR
Sbjct: 121 HHDSNRGMNKHHAKRLYKAVARFTSNDSNLTSEPGYVECMESPQQVNGYDCGLYVTAIAR 180

Query: 181 TICSWFEQRKDFHGDGLWFSAVVEEISPSLVADMRNEILGLIKSLMALKEYQPASK 236
           +ICSW+ QRK+  GDG WF AVVEE++P  VA+MR+EIL LI+SLMA+KEYQPAS+
Sbjct: 181 SICSWYAQRKEVDGDGGWFCAVVEEVTPCSVANMRSEILELIRSLMAVKEYQPASR 236

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BLAST of Lag0010034 vs. NCBI nr
Match: XP_023007377.1 (NEDD8-specific protease 1 [Cucurbita maxima])

HSP 1 Score: 402.9 bits (1034), Expect = 1.9e-108
Identity = 192/238 (80.67%), Postives = 212/238 (89.08%), Query Frame = 0


Query: 1   MWKSEGDKKILSYNDVVLRKSDLDILGGPYYLNDRIIEFYFSYLCTRHPSKDILLAPPSI 60
           M KSEGD+KILSYNDVVLRKSDLDIL GP YLNDRIIEFYFSYLCT HPS+DILL+PPSI
Sbjct: 1   MGKSEGDEKILSYNDVVLRKSDLDILNGPCYLNDRIIEFYFSYLCTCHPSEDILLSPPSI 60

Query: 61  AFWIMNCPDPESLNDFLEPLNLRDKKLVIFPVNDNNDVSKAEGGNHWSLLAFYREANVFV 120
           AFWIMNCPD + LNDFL+PLNLR + LVIFPVNDNNDVSKAEGGNHWS+L FYREANVFV
Sbjct: 61  AFWIMNCPDTDGLNDFLQPLNLRVRNLVIFPVNDNNDVSKAEGGNHWSVLVFYREANVFV 120

Query: 121 HHDSNKGMNKYHAKRLYKAVARFTGGSNSTSDPSYVECSESPQQVNGHDCGLYVTAIART 180
           HHDS KG+N   AKRLY+AVARF G +NSTS+PSYVECSESPQQVNGHDCGLYVTAI+R+
Sbjct: 121 HHDSCKGVNNSSAKRLYRAVARFMGVTNSTSEPSYVECSESPQQVNGHDCGLYVTAISRS 180

Query: 181 ICSWFEQRKDFHGDG---LWFSAVVEEISPSLVADMRNEILGLIKSLMALKEYQPASK 236
           IC W+EQ +   G G   LWFSAVV+E++PS VADMR+EILGLI+S MALKEYQPASK
Sbjct: 181 ICRWYEQERKEAGGGDGFLWFSAVVKEVTPSSVADMRSEILGLIRSFMALKEYQPASK 238

[Close]

BLAST of Lag0010034 vs. NCBI nr
Match: XP_023532288.1 (NEDD8-specific protease 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 402.5 bits (1033), Expect = 2.5e-108
Identity = 194/238 (81.51%), Postives = 215/238 (90.34%), Query Frame = 0


Query: 1   MWKSEGDKKILSYNDVVLRKSDLDILGGPYYLNDRIIEFYFSYLCTRHPSKDILLAPPSI 60
           M KSEGD+KILSYNDVVLRKSDLDIL GP YLNDRIIEFYFSYLCT HPS+DILL+PPSI
Sbjct: 1   MSKSEGDEKILSYNDVVLRKSDLDILNGPCYLNDRIIEFYFSYLCTCHPSEDILLSPPSI 60

Query: 61  AFWIMNCPDPESLNDFLEPLNLRDKKLVIFPVNDNNDVSKAEGGNHWSLLAFYREANVFV 120
           AFWIMNCPD + L DFL+PLNL ++ LVIFPVNDNNDVSKAEGGNHWS+L FYREANVFV
Sbjct: 61  AFWIMNCPDTDGLKDFLQPLNLLNRNLVIFPVNDNNDVSKAEGGNHWSVLVFYREANVFV 120

Query: 121 HHDSNKGMNKYHAKRLYKAVARFTGGSNSTSDPSYVECSESPQQVNGHDCGLYVTAIART 180
           HHDS KG+N   AKRLY+AVARF GG+NSTS+PSYVECSESPQQVNGHDCGLYVTAI+R+
Sbjct: 121 HHDSCKGVNNSSAKRLYRAVARFMGGTNSTSEPSYVECSESPQQVNGHDCGLYVTAISRS 180

Query: 181 ICSWFEQ-RKDFH-GDG-LWFSAVVEEISPSLVADMRNEILGLIKSLMALKEYQPASK 236
           IC W+EQ RK+   GDG LWFSAV +E++PSLVADMR+EILGLI+S MALKEYQPASK
Sbjct: 181 ICRWYEQERKEVGCGDGFLWFSAVAKEVTPSLVADMRSEILGLIRSFMALKEYQPASK 238

[Close]

BLAST of Lag0010034 vs. ExPASy Swiss-Prot
Match: Q9LSS7 (NEDD8-specific protease 1 OS=Arabidopsis thaliana OX=3702 GN=NEDP1 PE=2 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 3.5e-73
Identity = 134/226 (59.29%), Postives = 163/226 (72.12%), Query Frame = 0


Query: 1   MWKSEGDKKILSYNDVVLRKSDLDILGGPYYLNDRIIEFYFSYLCTRHPSKDILLAPPSI 60
           M  +  D KILSY DVVLR+SDLDIL GP +LNDR+IEFY S+L T H S  I L PPSI
Sbjct: 1   MGNTSDDDKILSYEDVVLRRSDLDILNGPIFLNDRVIEFYLSFLSTVHSSTTISLIPPSI 60

Query: 61  AFWIMNCPDPESLNDFLEPLNLRDKKLVIFPVNDNNDVSKAEGGNHWSLLAFYREANVFV 120
           AFWI NCPD E L DF++PLNLRDK L+I PVNDN++V  AEGG HWSLL +Y+EAN FV
Sbjct: 61  AFWISNCPDTEYLKDFMKPLNLRDKDLLILPVNDNSNVEVAEGGLHWSLLVYYKEANTFV 120

Query: 121 HHDSNKGMNKYHAKRLYKAVARFTGGSNSTSDPSYVECSESPQQVNGHDCGLYVTAIART 180
           HHDS  G+N++ AK+L+KAV+ F     S  D SY EC+++PQQ NG+DCG+++ A AR 
Sbjct: 121 HHDSYMGVNRWSAKQLFKAVSPFV----SNGDASYKECTDTPQQKNGYDCGVFLLATARV 180

Query: 181 ICSWFEQRKDFHGDGLWFSAVVEEISPSLVADMRNEILGLIKSLMA 227
           IC WF      + D LWF A V+E  P LV  +R EIL LIK LM+
Sbjct: 181 ICEWFSSGGMKNRDELWF-ANVKETVPDLVNHLREEILALIKKLMS 221

[Close]

BLAST of Lag0010034 vs. ExPASy Swiss-Prot
Match: Q96LD8 (Sentrin-specific protease 8 OS=Homo sapiens OX=9606 GN=SENP8 PE=1 SV=1)

HSP 1 Score: 127.9 bits (320), Expect = 1.5e-28
Identity = 73/222 (32.88%), Postives = 121/222 (54.50%), Query Frame = 0


Query: 7   DKKILSYNDVVLRKSDLDILGGPYYLNDRIIEFYFSYLCTR--HPSKD-ILLAPPSIAFW 66
           D  +LSY D +LR+SD+ +L  P +LND II F F Y      H   D +    P +  +
Sbjct: 2   DPVVLSYMDSLLRQSDVSLLDPPSWLNDHIIGFAFEYFANSQFHDCSDHVSFISPEVTQF 61

Query: 67  IMNCPDPESLNDFLEPLNLRDKKLVIFPVNDNNDVSKAEGGNHWSLLAFYREANVFVHHD 126
           I    +P  +  FLEPL+L +K++V   +NDN+  ++A GG HWSLL + ++ N F H+D
Sbjct: 62  IKCTSNPAEIAMFLEPLDLPNKRVVFLAINDNS--NQAAGGTHWSLLVYLQDKNSFFHYD 121

Query: 127 SNKGMNKYHAKRLYKAVARFTGGSNSTSDPSYVECSESPQQVNGHDCGLYVTAIARTIC- 186
           S+   N  HAK++ + +  F G        ++VE  ++P Q N +DCG+YV      +C 
Sbjct: 122 SHSRSNSVHAKQVAEKLEAFLGRKGDKL--AFVE-EKAPAQQNSYDCGMYVICNTEALCQ 181

Query: 187 SWFEQRKDFHGDGLWFSAVVEEISPSLVADMRNEILGLIKSL 225
           ++F Q+ +         ++++ ++P+ +   R E   LI +L
Sbjct: 182 NFFRQQTE---------SLLQLLTPAYITKKRGEWKDLITTL 209

[Close]

BLAST of Lag0010034 vs. ExPASy Swiss-Prot
Match: Q9D2Z4 (Sentrin-specific protease 8 OS=Mus musculus OX=10090 GN=Senp8 PE=1 SV=2)

HSP 1 Score: 125.6 bits (314), Expect = 7.6e-28
Identity = 73/226 (32.30%), Postives = 113/226 (50.00%), Query Frame = 0


Query: 7   DKKILSYNDVVLRKSDLDILGGPYYLNDRIIEFYFSYLCTR--HPSKD-ILLAPPSIAFW 66
           D  +LSY D +LR+SD+ +L  P +LND II F F Y      H   D +    P +  +
Sbjct: 2   DPVVLSYMDSLLRQSDVSLLDPPSWLNDHIIGFAFEYFANSQFHDCSDHVCFISPEVTQF 61

Query: 67  IMNCPDPESLNDFLEPLNLRDKKLVIFPVNDNNDVSKAEGGNHWSLLAFYREANVFVHHD 126
           I     P  +  FLEPL+L  K++V   +NDN+  ++A GG HWSLL + ++ N F H+D
Sbjct: 62  IKCTSSPAEIAMFLEPLDLPHKRVVFLAINDNS--NQAAGGTHWSLLVYLQDKNSFFHYD 121

Query: 127 SNKGMNKYHAKRLYKAVARFTGGSNSTSDPSYVECSESPQQVNGHDCGLYVTAIARTICS 186
           S+   N  HAK++ + +  F G   S  D       ++P Q N +DCG+YV      +C 
Sbjct: 122 SHSRSNSIHAKQVAEKLKAFLG---SKGDKLVFVEEKAPAQENSYDCGMYVICNTEALCQ 181

Query: 187 WFEQRKDFHGDGLWFSAVVEEISPSLVADMRNEILGLIKSLMALKE 230
              +R+          + ++ ++P+ +   R E   LI  L    E
Sbjct: 182 SLFRRQP--------ESPLQLLTPTYITKKRGEWKDLIARLAKKNE 214

[Close]

BLAST of Lag0010034 vs. ExPASy Swiss-Prot
Match: Q5FVJ8 (Sentrin-specific protease 8 OS=Rattus norvegicus OX=10116 GN=Senp8 PE=2 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 1.0e-27
Identity = 72/221 (32.58%), Postives = 113/221 (51.13%), Query Frame = 0


Query: 7   DKKILSYNDVVLRKSDLDILGGPYYLNDRIIEFYFSYLCTR--HPSKD-ILLAPPSIAFW 66
           D  +LSY D +LR+SD+ +L  P +LND II F F Y  +   H   D +    P +  +
Sbjct: 2   DPVVLSYMDSLLRQSDVSLLDPPNWLNDHIIGFAFEYFASSQFHDCSDHVCFISPEVTQF 61

Query: 67  IMNCPDPESLNDFLEPLNLRDKKLVIFPVNDNNDVSKAEGGNHWSLLAFYREANVFVHHD 126
           I     P  +  FLEPL+L  K++V   +NDN+  ++A GG HWSLL + ++ N F H+D
Sbjct: 62  IKCTSSPAEIAMFLEPLDLPHKRVVFLAINDNS--NQAAGGTHWSLLVYLQDKNSFFHYD 121

Query: 127 SNKGMNKYHAKRLYKAVARFTGGSNSTSDPSYVECSESPQQVNGHDCGLYVTAIARTICS 186
           S+   N  HAK++ + +  F G   S  D       ++P Q N +DCG+YV      +C 
Sbjct: 122 SHSRSNSIHAKQVAEKLKAFLG---SKGDKLVFVEEKAPAQQNSYDCGMYVICNTEALCQ 181

Query: 187 WFEQRKDFHGDGLWFSAVVEEISPSLVADMRNEILGLIKSL 225
              +R+          + ++ ++P+ +   R E   LI  L
Sbjct: 182 NLFRRQP--------ESPLQLLTPTYITKKRGEWKDLIARL 209

[Close]

BLAST of Lag0010034 vs. ExPASy Swiss-Prot
Match: Q54XR2 (Probable sentrin-specific protease 8 OS=Dictyostelium discoideum OX=44689 GN=senp8 PE=3 SV=1)

HSP 1 Score: 114.8 bits (286), Expect = 1.3e-24
Identity = 77/246 (31.30%), Postives = 126/246 (51.22%), Query Frame = 0


Query: 7   DKKILSYNDVVLRKSDLDILGGPYYLNDRIIEFYFSYL-------CTRHPSKDILLAPPS 66
           D  I+SYND  + +SD++IL    +LND II FY  +L         + P++ +LL  PS
Sbjct: 3   DPLIVSYNDSAIYQSDINILKSNQWLNDSIISFYLEWLKDGGEDNKNKIPNQ-VLLLSPS 62

Query: 67  IAFWIMNCPDPESLND----FLEPLNLRDKKLVIFPVNDNNDVSKAEGGNHWSLLAFYRE 126
           + F    C   ES  +    F +PL+L+ K+++ FP+ +N D +   GG HWSLL F + 
Sbjct: 63  VVFC---CSFVESEQEIQLMFEQPLSLKTKEVIFFPLTNNRDPNVIGGGTHWSLLIFIKS 122

Query: 127 ANVFVHHDSNKGMNKYHAKRLYKAVARF----TGGSNSTSDPSYVECSESPQQVNGHDCG 186
            N F+++DS   +N +++      +++F    +     T+   ++   ++PQQ NG+DCG
Sbjct: 123 LNKFIYYDS---INSFNSSDAIFIISKFKFLLSSPPPKTNLKEFLINQKTPQQQNGYDCG 182

Query: 187 LYVTAIARTICSWF---------EQRKDFHGDGLWF----SAVVEEISPSLVADMRNEIL 225
           LYV +I   +             E+ K  + D L        + +EI+P+ + D R EIL
Sbjct: 183 LYVLSIIEELLKLIIKENENNKGEENKISYKDLLLSEFTNELLFKEITPNYIKDKRVEIL 241

[Close]

BLAST of Lag0010034 vs. ExPASy TrEMBL
Match: A0A5D3CSG4 (NEDD8-specific protease 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold84G00810 PE=3 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 2.8e-110
Identity = 194/236 (82.20%), Postives = 214/236 (90.68%), Query Frame = 0


Query: 1   MWKSEGDKKILSYNDVVLRKSDLDILGGPYYLNDRIIEFYFSYLCTRHPSKDILLAPPSI 60
           M K EGD+KILS+NDVVLRKSDL+IL GPY+LNDRIIEFYFSYL T HPS DILL+PPSI
Sbjct: 1   MGKFEGDEKILSFNDVVLRKSDLEILSGPYFLNDRIIEFYFSYLSTTHPSNDILLSPPSI 60

Query: 61  AFWIMNCPDPESLNDFLEPLNLRDKKLVIFPVNDNNDVSKAEGGNHWSLLAFYREANVFV 120
           AFWIMNCPD ESLN FLEPLNLR KKLVIFPVNDN+DVSK EGGNHWSLLAFYREAN+FV
Sbjct: 61  AFWIMNCPDVESLNGFLEPLNLRHKKLVIFPVNDNSDVSKPEGGNHWSLLAFYREANIFV 120

Query: 121 HHDSNKGMNKYHAKRLYKAVARF-TGGSNSTSDPSYVECSESPQQVNGHDCGLYVTAIAR 180
           HHDSNKGMNKY AKRLYKAVARF   GSNSTS+PSY+EC ESPQQVNG+DCG+YVTAIAR
Sbjct: 121 HHDSNKGMNKYDAKRLYKAVARFMNDGSNSTSNPSYMECVESPQQVNGYDCGVYVTAIAR 180

Query: 181 TICSWFEQRKDFHGDGLWFSAVVEEISPSLVADMRNEILGLIKSLMALKEYQPASK 236
           +IC W+E+R++   DGLWFSAVVEEISP LVA+MR EIL LI+SLMA+KEYQPA+K
Sbjct: 181 SICKWYEERREVEKDGLWFSAVVEEISPLLVANMRREILELIRSLMAVKEYQPATK 236

[Close]

BLAST of Lag0010034 vs. ExPASy TrEMBL
Match: A0A1S3CK81 (NEDD8-specific protease 1 OS=Cucumis melo OX=3656 GN=LOC103501907 PE=3 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 2.8e-110
Identity = 194/236 (82.20%), Postives = 214/236 (90.68%), Query Frame = 0


Query: 1   MWKSEGDKKILSYNDVVLRKSDLDILGGPYYLNDRIIEFYFSYLCTRHPSKDILLAPPSI 60
           M K EGD+KILS+NDVVLRKSDL+IL GPY+LNDRIIEFYFSYL T HPS DILL+PPSI
Sbjct: 1   MGKFEGDEKILSFNDVVLRKSDLEILSGPYFLNDRIIEFYFSYLSTTHPSNDILLSPPSI 60

Query: 61  AFWIMNCPDPESLNDFLEPLNLRDKKLVIFPVNDNNDVSKAEGGNHWSLLAFYREANVFV 120
           AFWIMNCPD ESLN FLEPLNLR KKLVIFPVNDN+DVSK EGGNHWSLLAFYREAN+FV
Sbjct: 61  AFWIMNCPDVESLNGFLEPLNLRHKKLVIFPVNDNSDVSKPEGGNHWSLLAFYREANIFV 120

Query: 121 HHDSNKGMNKYHAKRLYKAVARF-TGGSNSTSDPSYVECSESPQQVNGHDCGLYVTAIAR 180
           HHDSNKGMNKY AKRLYKAVARF   GSNSTS+PSY+EC ESPQQVNG+DCG+YVTAIAR
Sbjct: 121 HHDSNKGMNKYDAKRLYKAVARFMNDGSNSTSNPSYMECVESPQQVNGYDCGVYVTAIAR 180

Query: 181 TICSWFEQRKDFHGDGLWFSAVVEEISPSLVADMRNEILGLIKSLMALKEYQPASK 236
           +IC W+E+R++   DGLWFSAVVEEISP LVA+MR EIL LI+SLMA+KEYQPA+K
Sbjct: 181 SICKWYEERREVEKDGLWFSAVVEEISPLLVANMRREILELIRSLMAVKEYQPATK 236

[Close]

BLAST of Lag0010034 vs. ExPASy TrEMBL
Match: A0A6J1CWB2 (NEDD8-specific protease 1 OS=Momordica charantia OX=3673 GN=LOC111015386 PE=3 SV=1)

HSP 1 Score: 403.3 bits (1035), Expect = 7.0e-109
Identity = 191/236 (80.93%), Postives = 212/236 (89.83%), Query Frame = 0


Query: 1   MWKSEGDKKILSYNDVVLRKSDLDILGGPYYLNDRIIEFYFSYLCTRHPSKDILLAPPSI 60
           M KSEGDKKILSYNDVVLRKSDLDIL GPYYLNDRIIEFYFSYL + HPSKDILLAPPSI
Sbjct: 1   MRKSEGDKKILSYNDVVLRKSDLDILSGPYYLNDRIIEFYFSYLSSYHPSKDILLAPPSI 60

Query: 61  AFWIMNCPDPESLNDFLEPLNLRDKKLVIFPVNDNNDVSKAEGGNHWSLLAFYREANVFV 120
           AFW MNCPD  SL+DFL PL+L +KKLVIFPVNDN+DVSKAEGGNHWSLLAFYRE+N F+
Sbjct: 61  AFWTMNCPDSNSLDDFLRPLDLFNKKLVIFPVNDNDDVSKAEGGNHWSLLAFYRESNTFI 120

Query: 121 HHDSNKGMNKYHAKRLYKAVARFTGG-SNSTSDPSYVECSESPQQVNGHDCGLYVTAIAR 180
           HHDSN+GMNK+HAKRLYKAVARFT   SN TS+P YVEC ESPQQVNG+DCGLYVTAIAR
Sbjct: 121 HHDSNRGMNKHHAKRLYKAVARFTSNDSNLTSEPGYVECMESPQQVNGYDCGLYVTAIAR 180

Query: 181 TICSWFEQRKDFHGDGLWFSAVVEEISPSLVADMRNEILGLIKSLMALKEYQPASK 236
           +ICSW+ QRK+  GDG WF AVVEE++P  VA+MR+EIL LI+SLMA+KEYQPAS+
Sbjct: 181 SICSWYAQRKEVDGDGGWFCAVVEEVTPCSVANMRSEILELIRSLMAVKEYQPASR 236

[Close]

BLAST of Lag0010034 vs. ExPASy TrEMBL
Match: A0A6J1KYI9 (NEDD8-specific protease 1 OS=Cucurbita maxima OX=3661 GN=LOC111499890 PE=3 SV=1)

HSP 1 Score: 402.9 bits (1034), Expect = 9.1e-109
Identity = 192/238 (80.67%), Postives = 212/238 (89.08%), Query Frame = 0


Query: 1   MWKSEGDKKILSYNDVVLRKSDLDILGGPYYLNDRIIEFYFSYLCTRHPSKDILLAPPSI 60
           M KSEGD+KILSYNDVVLRKSDLDIL GP YLNDRIIEFYFSYLCT HPS+DILL+PPSI
Sbjct: 1   MGKSEGDEKILSYNDVVLRKSDLDILNGPCYLNDRIIEFYFSYLCTCHPSEDILLSPPSI 60

Query: 61  AFWIMNCPDPESLNDFLEPLNLRDKKLVIFPVNDNNDVSKAEGGNHWSLLAFYREANVFV 120
           AFWIMNCPD + LNDFL+PLNLR + LVIFPVNDNNDVSKAEGGNHWS+L FYREANVFV
Sbjct: 61  AFWIMNCPDTDGLNDFLQPLNLRVRNLVIFPVNDNNDVSKAEGGNHWSVLVFYREANVFV 120

Query: 121 HHDSNKGMNKYHAKRLYKAVARFTGGSNSTSDPSYVECSESPQQVNGHDCGLYVTAIART 180
           HHDS KG+N   AKRLY+AVARF G +NSTS+PSYVECSESPQQVNGHDCGLYVTAI+R+
Sbjct: 121 HHDSCKGVNNSSAKRLYRAVARFMGVTNSTSEPSYVECSESPQQVNGHDCGLYVTAISRS 180

Query: 181 ICSWFEQRKDFHGDG---LWFSAVVEEISPSLVADMRNEILGLIKSLMALKEYQPASK 236
           IC W+EQ +   G G   LWFSAVV+E++PS VADMR+EILGLI+S MALKEYQPASK
Sbjct: 181 ICRWYEQERKEAGGGDGFLWFSAVVKEVTPSSVADMRSEILGLIRSFMALKEYQPASK 238

[Close]

BLAST of Lag0010034 vs. ExPASy TrEMBL
Match: A0A0A0KEM9 (ULP_PROTEASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G289720 PE=3 SV=1)

HSP 1 Score: 402.5 bits (1033), Expect = 1.2e-108
Identity = 193/236 (81.78%), Postives = 211/236 (89.41%), Query Frame = 0


Query: 1   MWKSEGDKKILSYNDVVLRKSDLDILGGPYYLNDRIIEFYFSYLCTRHPSKDILLAPPSI 60
           M KSEGD+KILSYNDVVLRKSDL+IL GPY+LNDRIIEFYFSYL T HPS  ILL+PPSI
Sbjct: 1   MGKSEGDEKILSYNDVVLRKSDLEILSGPYFLNDRIIEFYFSYLSTTHPSNGILLSPPSI 60

Query: 61  AFWIMNCPDPESLNDFLEPLNLRDKKLVIFPVNDNNDVSKAEGGNHWSLLAFYREANVFV 120
           AFW+MNCPD ESLN FLEPLNL  KKLVIFPVNDN DVSK EGGNHWSLLAFYREAN+FV
Sbjct: 61  AFWMMNCPDVESLNSFLEPLNLPHKKLVIFPVNDNIDVSKPEGGNHWSLLAFYREANIFV 120

Query: 121 HHDSNKGMNKYHAKRLYKAVARF-TGGSNSTSDPSYVECSESPQQVNGHDCGLYVTAIAR 180
           HHDSNKGMNKY AKRLY AVARF   GS ST +PSYVEC ESPQQVNG+DCG+YVTAIAR
Sbjct: 121 HHDSNKGMNKYAAKRLYNAVARFMNDGSISTLNPSYVECVESPQQVNGYDCGVYVTAIAR 180

Query: 181 TICSWFEQRKDFHGDGLWFSAVVEEISPSLVADMRNEILGLIKSLMALKEYQPASK 236
           +IC W+E+R++   DGLWFSAVVEEISPSLVA+MR EILGLI+SLMA+KEYQPA+K
Sbjct: 181 SICKWYEERREVEKDGLWFSAVVEEISPSLVANMRREILGLIRSLMAVKEYQPATK 236

[Close]

BLAST of Lag0010034 vs. TAIR 10
Match: AT5G60190.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 276.2 bits (705), Expect = 2.5e-74
Identity = 134/226 (59.29%), Postives = 163/226 (72.12%), Query Frame = 0


Query: 1   MWKSEGDKKILSYNDVVLRKSDLDILGGPYYLNDRIIEFYFSYLCTRHPSKDILLAPPSI 60
           M  +  D KILSY DVVLR+SDLDIL GP +LNDR+IEFY S+L T H S  I L PPSI
Sbjct: 1   MGNTSDDDKILSYEDVVLRRSDLDILNGPIFLNDRVIEFYLSFLSTVHSSTTISLIPPSI 60

Query: 61  AFWIMNCPDPESLNDFLEPLNLRDKKLVIFPVNDNNDVSKAEGGNHWSLLAFYREANVFV 120
           AFWI NCPD E L DF++PLNLRDK L+I PVNDN++V  AEGG HWSLL +Y+EAN FV
Sbjct: 61  AFWISNCPDTEYLKDFMKPLNLRDKDLLILPVNDNSNVEVAEGGLHWSLLVYYKEANTFV 120

Query: 121 HHDSNKGMNKYHAKRLYKAVARFTGGSNSTSDPSYVECSESPQQVNGHDCGLYVTAIART 180
           HHDS  G+N++ AK+L+KAV+ F     S  D SY EC+++PQQ NG+DCG+++ A AR 
Sbjct: 121 HHDSYMGVNRWSAKQLFKAVSPFV----SNGDASYKECTDTPQQKNGYDCGVFLLATARV 180

Query: 181 ICSWFEQRKDFHGDGLWFSAVVEEISPSLVADMRNEILGLIKSLMA 227
           IC WF      + D LWF A V+E  P LV  +R EIL LIK LM+
Sbjct: 181 ICEWFSSGGMKNRDELWF-ANVKETVPDLVNHLREEILALIKKLMS 221

[Close]

BLAST of Lag0010034 vs. TAIR 10
Match: AT4G33620.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 44.7 bits (104), Expect = 1.2e-04
Identity = 48/209 (22.97%), Postives = 78/209 (37.32%), Query Frame = 0


Query: 16  VVLRKSDLDILGGPYYLNDRIIEFYFSYLCTRHPSKDILLAPPSIAFWIMNC-------- 75
           VV+RK D+++L    ++ND II+FY  YL  R   K+         F   NC        
Sbjct: 305 VVVRKQDIELLKPRRFINDTIIDFYIKYLKNRISPKE------RGRFHFFNCFFFRKLAN 364

Query: 76  -----PDP-------ESLNDFLEPLNLRDKKLVIFPVNDNNDVSKAEGGNHWSLLAFYRE 135
                P         + +  + + ++L +K  +  P+N +          HWSL+     
Sbjct: 365 LDKGTPSTCGGREAYQRVQKWTKNVDLFEKDYIFIPINCS---------FHWSLVIICHP 424

Query: 136 ANV----------------------FVHHDSNKGMNKYHAKRLYKAVAR---FTGGSNST 175
             +                       +H DS KG +K     ++ +  R        N+T
Sbjct: 425 GELVPSHVNFHSFDDEVENPQRVPCILHLDSIKGSHKGGLINIFPSYLREEWKARHENTT 484

The following BLAST results are available for this feature:

BLAST of Lag0010034 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038902669.1	6.7e-114	84.75	NEDD8-specific protease 1 [Benincasa hispida]	[more]
XP_008463889.1	5.9e-110	82.20	PREDICTED: NEDD8-specific protease 1 [Cucumis melo] >KAA0035294.1 NEDD8-specific...	[more]
XP_022146090.1	1.4e-108	80.93	NEDD8-specific protease 1 [Momordica charantia]	[more]
XP_023007377.1	1.9e-108	80.67	NEDD8-specific protease 1 [Cucurbita maxima]	[more]
XP_023532288.1	2.5e-108	81.51	NEDD8-specific protease 1 [Cucurbita pepo subsp. pepo]	[more]

BLAST of Lag0010034 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LSS7	3.5e-73	59.29	NEDD8-specific protease 1 OS=Arabidopsis thaliana OX=3702 GN=NEDP1 PE=2 SV=1	[more]
Q96LD8	1.5e-28	32.88	Sentrin-specific protease 8 OS=Homo sapiens OX=9606 GN=SENP8 PE=1 SV=1	[more]
Q9D2Z4	7.6e-28	32.30	Sentrin-specific protease 8 OS=Mus musculus OX=10090 GN=Senp8 PE=1 SV=2	[more]
Q5FVJ8	1.0e-27	32.58	Sentrin-specific protease 8 OS=Rattus norvegicus OX=10116 GN=Senp8 PE=2 SV=1	[more]
Q54XR2	1.3e-24	31.30	Probable sentrin-specific protease 8 OS=Dictyostelium discoideum OX=44689 GN=sen...	[more]

BLAST of Lag0010034 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3CSG4	2.8e-110	82.20	NEDD8-specific protease 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]
A0A1S3CK81	2.8e-110	82.20	NEDD8-specific protease 1 OS=Cucumis melo OX=3656 GN=LOC103501907 PE=3 SV=1	[more]
A0A6J1CWB2	7.0e-109	80.93	NEDD8-specific protease 1 OS=Momordica charantia OX=3673 GN=LOC111015386 PE=3 SV...	[more]
A0A6J1KYI9	9.1e-109	80.67	NEDD8-specific protease 1 OS=Cucurbita maxima OX=3661 GN=LOC111499890 PE=3 SV=1	[more]
A0A0A0KEM9	1.2e-108	81.78	ULP_PROTEASE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G28972...	[more]

BLAST of Lag0010034 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G60190.1	2.5e-74	59.29	Cysteine proteinases superfamily protein	[more]
AT4G33620.1	1.2e-04	22.97	Cysteine proteinases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003653	Ulp1 protease family, C-terminal catalytic domain	PFAM	PF02902	Peptidase_C48	coord: 31..189 e-value: 9.4E-12 score: 45.2
IPR003653	Ulp1 protease family, C-terminal catalytic domain	PROSITE	PS50600	ULP_PROTEASE	coord: 16..181 score: 13.930004
None	No IPR available	GENE3D	3.40.395.10	Adenoviral Proteinase; Chain A	coord: 7..227 e-value: 1.8E-63 score: 216.1
None	No IPR available	PANTHER	PTHR46468:SF2	PROTEASE, SENP8, PUTATIVE-RELATED	coord: 159..225
None	No IPR available	PANTHER	PTHR46468:SF2	PROTEASE, SENP8, PUTATIVE-RELATED	coord: 8..152
IPR044613	NEDD8-specific protease 1/2-like	PANTHER	PTHR46468	SENTRIN-SPECIFIC PROTEASE 8	coord: 159..225
IPR044613	NEDD8-specific protease 1/2-like	PANTHER	PTHR46468	SENTRIN-SPECIFIC PROTEASE 8	coord: 8..152
IPR038765	Papain-like cysteine peptidase superfamily	SUPERFAMILY	54001	Cysteine proteinases	coord: 7..225

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0010034.1	Lag0010034.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006508	proteolysis
molecular_function	GO:0019784	NEDD8-specific protease activity
molecular_function	GO:0008234	cysteine-type peptidase activity