Lag0009994 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0009994
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	RING-type E3 ubiquitin transferase
Location	chr9: 43779082 .. 43779717 (+)
RNA-Seq Expression	Lag0009994
Synteny	Lag0009994

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCCATCGAGTCCACCGCCGCCATCTCCGGTGCCTGATTACTCTAGCTGGAGTCCGTGGTCAATCTCGACCATCGCCATCGTATGCACCGTCTTTCTACTGTTAAGTAACTACAGAATATTGAGACAACTGTGCCGTGTCCTTTATGGATTGATTACCGGTCGACCTAGGGTCCAATTTGAGGAAAACGCTAACGATCCTTCTCCGCAAATCCATGGCCATGGATTGGAAGCCTCGACGATTCGTTTGCTACCTATCTCACAGTTCAAAAAGAATGAAGAATCCGAATCAACAACAAGCACCAATACGGAATGCGCTGTTTGTTTGGGCGAATTCGAAGAAGGGGAATTGATTAAACACTTACCAAATTGCAATCACTCGTTTCATAGTTCTTGTATCGACGCCTGGTTCCGTGACCATTCGAGTTGCCCTCTCTGCAGATCGCGCGTCTTGAGATTCTCCGTCGATTGCCCTAATTCTGATTCTTCTTCGGCAATGCTGGAGACGCTCGGAAGAGAAGACGTTCTACGAGAAAGAGTAGCGCATTATCAAACACTTCGTGCTCAGATTCTGCAGAGCTCGGAATTGAGAAGAGATGCATCACCGGTTCGAGGATCGGAACAACAAAATCGATGA

mRNA sequence

ATGCCATCGAGTCCACCGCCGCCATCTCCGGTGCCTGATTACTCTAGCTGGAGTCCGTGGTCAATCTCGACCATCGCCATCGTATGCACCGTCTTTCTACTGTTAAGTAACTACAGAATATTGAGACAACTGTGCCGTGTCCTTTATGGATTGATTACCGGTCGACCTAGGGTCCAATTTGAGGAAAACGCTAACGATCCTTCTCCGCAAATCCATGGCCATGGATTGGAAGCCTCGACGATTCGTTTGCTACCTATCTCACAGTTCAAAAAGAATGAAGAATCCGAATCAACAACAAGCACCAATACGGAATGCGCTGTTTGTTTGGGCGAATTCGAAGAAGGGGAATTGATTAAACACTTACCAAATTGCAATCACTCGTTTCATAGTTCTTGTATCGACGCCTGGTTCCGTGACCATTCGAGTTGCCCTCTCTGCAGATCGCGCGTCTTGAGATTCTCCGTCGATTGCCCTAATTCTGATTCTTCTTCGGCAATGCTGGAGACGCTCGGAAGAGAAGACGTTCTACGAGAAAGAGTAGCGCATTATCAAACACTTCGTGCTCAGATTCTGCAGAGCTCGGAATTGAGAAGAGATGCATCACCGGTTCGAGGATCGGAACAACAAAATCGATGA

Coding sequence (CDS)

ATGCCATCGAGTCCACCGCCGCCATCTCCGGTGCCTGATTACTCTAGCTGGAGTCCGTGGTCAATCTCGACCATCGCCATCGTATGCACCGTCTTTCTACTGTTAAGTAACTACAGAATATTGAGACAACTGTGCCGTGTCCTTTATGGATTGATTACCGGTCGACCTAGGGTCCAATTTGAGGAAAACGCTAACGATCCTTCTCCGCAAATCCATGGCCATGGATTGGAAGCCTCGACGATTCGTTTGCTACCTATCTCACAGTTCAAAAAGAATGAAGAATCCGAATCAACAACAAGCACCAATACGGAATGCGCTGTTTGTTTGGGCGAATTCGAAGAAGGGGAATTGATTAAACACTTACCAAATTGCAATCACTCGTTTCATAGTTCTTGTATCGACGCCTGGTTCCGTGACCATTCGAGTTGCCCTCTCTGCAGATCGCGCGTCTTGAGATTCTCCGTCGATTGCCCTAATTCTGATTCTTCTTCGGCAATGCTGGAGACGCTCGGAAGAGAAGACGTTCTACGAGAAAGAGTAGCGCATTATCAAACACTTCGTGCTCAGATTCTGCAGAGCTCGGAATTGAGAAGAGATGCATCACCGGTTCGAGGATCGGAACAACAAAATCGATGA

Protein sequence

MPSSPPPPSPVPDYSSWSPWSISTIAIVCTVFLLLSNYRILRQLCRVLYGLITGRPRVQFEENANDPSPQIHGHGLEASTIRLLPISQFKKNEESESTTSTNTECAVCLGEFEEGELIKHLPNCNHSFHSSCIDAWFRDHSSCPLCRSRVLRFSVDCPNSDSSSAMLETLGREDVLRERVAHYQTLRAQILQSSELRRDASPVRGSEQQNR

Homology

[Close]

BLAST of Lag0009994 vs. NCBI nr
Match: XP_031743630.1 (RING-H2 finger protein ATL52-like [Cucumis sativus] >KGN47355.1 hypothetical protein Csa_023057 [Cucumis sativus])

HSP 1 Score: 347.8 bits (891), Expect = 6.5e-92
Identity = 177/211 (83.89%), Postives = 191/211 (90.52%), Query Frame = 0


Query: 1   MPSSPPPPSPVPDYSSWSPWSISTIAIVCTVFLLLSNYRILRQLCRVLYGLITGRPRVQF 60
           MPSSPPPPSPVPDYSSWSPWSISTI +VC VFLLLSNYR+L+QLCRVL+GL TGRP VQF
Sbjct: 1   MPSSPPPPSPVPDYSSWSPWSISTIVVVCIVFLLLSNYRLLKQLCRVLHGLFTGRPTVQF 60

Query: 61  EENANDPSPQIHGHGLEASTIRLLPISQFKKNEESESTTSTNTECAVCLGEFEEGELIKH 120
           EEN N+PS QIHGHGLE S IRLLPISQFKKNEESESTTS NTECAVCLGEFEEGELIKH
Sbjct: 61  EENPNNPSLQIHGHGLETSMIRLLPISQFKKNEESESTTSFNTECAVCLGEFEEGELIKH 120

Query: 121 LPNCNHSFHSSCIDAWFRDHSSCPLCRSRVLRFSVDCPNSDSSSAMLETLGREDVLRERV 180
           LPNCNHSFHS CIDAWFR+HSSCPLCR +VL FS   P+S SSSAMLETLGRE+VLRERV
Sbjct: 121 LPNCNHSFHSPCIDAWFRNHSSCPLCRLQVLSFST--PDS-SSSAMLETLGRENVLRERV 180

Query: 181 AHYQTLRAQILQSSELRRDASPVRGSEQQNR 212
           AHYQTLRAQILQ  E RRDAS ++G++Q+NR
Sbjct: 181 AHYQTLRAQILQHPEFRRDAS-IQGTDQRNR 207

[Close]

BLAST of Lag0009994 vs. NCBI nr
Match: KAG6570731.1 (RING-H2 finger protein ATL16, partial [Cucurbita argyrosperma subsp. sororia] >KAG7010575.1 RING-H2 finger protein ATL16, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 338.2 bits (866), Expect = 5.1e-89
Identity = 172/214 (80.37%), Postives = 188/214 (87.85%), Query Frame = 0


Query: 1   MPSS---PPPPSPVPDYSSWSPWSISTIAIVCTVFLLLSNYRILRQLCRVLYGLITGRPR 60
           MPSS   PPPPSPVPDYSSWSPWSIS IAIVC V LLLSNYRIL++ CRVL+GL  GR  
Sbjct: 1   MPSSPPPPPPPSPVPDYSSWSPWSISIIAIVCIVVLLLSNYRILKRWCRVLHGLFCGRFV 60

Query: 61  VQFEENANDPSPQIHGHGLEASTIRLLPISQFKKNEESESTTSTNTECAVCLGEFEEGEL 120
           VQF+EN NDPSP++H HGLEASTIRLLPISQFKKNEE+EST S NTECAVCLGEFEEGEL
Sbjct: 61  VQFDENPNDPSPEVHVHGLEASTIRLLPISQFKKNEEAESTASINTECAVCLGEFEEGEL 120

Query: 121 IKHLPNCNHSFHSSCIDAWFRDHSSCPLCRSRVLRFSVDCPNSDSSSAMLETLGREDVLR 180
           IK+LPNCNHSFHSSCIDAW R+HSSCPLCR  V   S+DCP  DSSSAMLETLGREDV R
Sbjct: 121 IKNLPNCNHSFHSSCIDAWLRNHSSCPLCRLHVSNLSIDCPTPDSSSAMLETLGREDVER 180

Query: 181 ERVAHYQTLRAQILQSSELRRDASPVRGSEQQNR 212
           ERV++YQTLRAQILQ+ ELRRDAS V+GS+Q+NR
Sbjct: 181 ERVSYYQTLRAQILQNPELRRDAS-VQGSDQRNR 213

[Close]

BLAST of Lag0009994 vs. NCBI nr
Match: XP_008448367.1 (PREDICTED: RING-H2 finger protein ATL66-like [Cucumis melo] >KAA0061954.1 RING-H2 finger protein ATL66-like [Cucumis melo var. makuwa] >TYK23976.1 RING-H2 finger protein ATL66-like [Cucumis melo var. makuwa])

HSP 1 Score: 334.3 bits (856), Expect = 7.4e-88
Identity = 169/210 (80.48%), Postives = 184/210 (87.62%), Query Frame = 0


Query: 1   MPSSPPPPSPVPDYSSWSPWSISTIAIVCTVFLLLSNYRILRQLCRVLYGLITGRPRVQF 60
           MPSSPPPPSPVPDYSSWSPWSISTI IVC +FLLLSNYR+L+QLCRVL+ L +GRP VQF
Sbjct: 1   MPSSPPPPSPVPDYSSWSPWSISTIVIVCVIFLLLSNYRLLKQLCRVLHELFSGRPAVQF 60

Query: 61  EENANDPSPQIHGHGLEASTIRLLPISQFKKNEESESTTSTNTECAVCLGEFEEGELIKH 120
           EEN N+PS QIH HGLE S IRLLPISQFKK EESESTTS NTECAVCLGEFEEGELIK+
Sbjct: 61  EENPNNPSLQIHAHGLETSMIRLLPISQFKKKEESESTTSFNTECAVCLGEFEEGELIKN 120

Query: 121 LPNCNHSFHSSCIDAWFRDHSSCPLCRSRVLRFSVDCPNSDSSSAMLETLGREDVLRERV 180
           LPNCNHSFHSSCIDAWFRDHSSCPLCR +V  FS      DSSSAMLETLGRE++LRERV
Sbjct: 121 LPNCNHSFHSSCIDAWFRDHSSCPLCRLQVWSFS----TPDSSSAMLETLGRENILRERV 180

Query: 181 AHYQTLRAQILQSSELRRDASPVRGSEQQN 211
           AHYQTLRAQILQ  E RRD S ++GS+Q++
Sbjct: 181 AHYQTLRAQILQHPEFRRDES-IQGSDQRD 205

[Close]

BLAST of Lag0009994 vs. NCBI nr
Match: XP_023512261.1 (RING-H2 finger protein ATL74-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 332.4 bits (851), Expect = 2.8e-87
Identity = 169/214 (78.97%), Postives = 185/214 (86.45%), Query Frame = 0


Query: 1   MPSS---PPPPSPVPDYSSWSPWSISTIAIVCTVFLLLSNYRILRQLCRVLYGLITGRPR 60
           MPSS   PPPPSPVPDYSSWSPWSIS IAIVC V LLLSNYRIL++ CRV +GL  GR  
Sbjct: 1   MPSSPPPPPPPSPVPDYSSWSPWSISIIAIVCIVVLLLSNYRILKRWCRVFHGLFCGRSV 60

Query: 61  VQFEENANDPSPQIHGHGLEASTIRLLPISQFKKNEESESTTSTNTECAVCLGEFEEGEL 120
           VQF+EN NDPSPQ+H HGLEASTIRLLPISQFKKNEE+EST S NTECAVCLGEFEEGEL
Sbjct: 61  VQFDENPNDPSPQVHVHGLEASTIRLLPISQFKKNEEAESTASINTECAVCLGEFEEGEL 120

Query: 121 IKHLPNCNHSFHSSCIDAWFRDHSSCPLCRSRVLRFSVDCPNSDSSSAMLETLGREDVLR 180
           IK+LPNCNHSFHSSCIDAW R+HSSCPLCR  V   S+DCP  DSSSAMLETL REDV  
Sbjct: 121 IKNLPNCNHSFHSSCIDAWLRNHSSCPLCRLHVSNLSIDCPTPDSSSAMLETLSREDVDG 180

Query: 181 ERVAHYQTLRAQILQSSELRRDASPVRGSEQQNR 212
           ERV++YQTLRAQILQ+ +LRRDAS V+GS+Q+NR
Sbjct: 181 ERVSYYQTLRAQILQNPDLRRDAS-VQGSDQRNR 213

[Close]

BLAST of Lag0009994 vs. NCBI nr
Match: XP_031247518.1 (RING-H2 finger protein ATL16-like [Pistacia vera])

HSP 1 Score: 186.8 bits (473), Expect = 1.9e-43
Identity = 97/198 (48.99%), Postives = 123/198 (62.12%), Query Frame = 0


Query: 3   SSPPPPSPVPDYSSWSPWSISTIAIVCTVFLLLSNYRILRQLCRVLYGLITGR----PRV 62
           SS P P+P+    SW+P  +S +A+ CT+FLL S Y  L++LC   +     R     ++
Sbjct: 2   SSSPSPAPIYKPPSWNPLVVSLVALTCTIFLLFSYYNYLKKLCCCAFNRGNSRNPDQRQL 61

Query: 63  QFEENANDPSPQIHGHGLEASTIRLLPISQFKKN---EESESTTSTNTECAVCLGEFEEG 122
             E N +DPS Q + HGLE+S I  LPI+QFKKN   EE E     N+EC VCLGEFEEG
Sbjct: 62  LNEANFDDPSLQFNSHGLESSIIHSLPITQFKKNINKEEEEELQPRNSECGVCLGEFEEG 121

Query: 123 ELIKHLPNCNHSFHSSCIDAWFRDHSSCPLCRSRVLRFSVDCPNSDSSSAMLETLGREDV 182
           E +KHLPNC+H+FH  CID WF  HS+CPLCRS+    ++    S S   +LE L RED 
Sbjct: 122 EWLKHLPNCSHAFHIPCIDTWFHSHSNCPLCRSQAFDLTIPHECSVSMYTLLENLRREDF 181

Query: 183 LRERVAHYQTLRAQILQS 194
             ER  HYQ LR  +LQS
Sbjct: 182 FHERATHYQILRTAVLQS 199

[Close]

BLAST of Lag0009994 vs. ExPASy Swiss-Prot
Match: Q9LSW9 (RING-H2 finger protein ATL16 OS=Arabidopsis thaliana OX=3702 GN=ATL16 PE=2 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 1.8e-20
Identity = 60/167 (35.93%), Postives = 93/167 (55.69%), Query Frame = 0


Query: 5   PPPPSPV-----PDYSSWSPWSISTIAIVCTVFLLLSNYRILRQLCRVLYGL-ITGRPRV 64
           PPPP P+        +S+   +++ I I+ T FLL+S Y  + + C   + + I GR  +
Sbjct: 17  PPPPPPIFHRASSTGTSFPILAVAVIGILATAFLLVSYYVFVIKCCLNWHRIDILGRFSL 76

Query: 65  -QFEENANDP----SPQIHGHGLEASTIRLLPISQFKK----------NEESESTTSTNT 124
            +   N  DP    SP++   GL+ S IR +PI +FKK           E  E     + 
Sbjct: 77  SRRRRNDQDPLMVYSPELRSRGLDESVIRAIPIFKFKKRYDQNDGVFTGEGEEEEEKRSQ 136

Query: 125 ECAVCLGEFEEGELIKHLPNCNHSFHSSCIDAWFRDHSSCPLCRSRV 151
           EC+VCL EF++ E ++ +PNC+H FH  CID W +++++CPLCR+RV
Sbjct: 137 ECSVCLSEFQDEEKLRIIPNCSHLFHIDCIDVWLQNNANCPLCRTRV 183

[Close]

BLAST of Lag0009994 vs. ExPASy Swiss-Prot
Match: P93823 (RING-H2 finger protein ATL1 OS=Arabidopsis thaliana OX=3702 GN=ATL1 PE=2 SV=1)

HSP 1 Score: 94.0 bits (232), Expect = 2.2e-18
Identity = 62/180 (34.44%), Postives = 94/180 (52.22%), Query Frame = 0


Query: 6   PPPSPVPDYSSWSP--------------WSISTIAIVCTVFLLLSNYRILRQLCRVLYGL 65
           PPP P P  +  SP               +I+ I I+ T FLL+S Y  + + C   + +
Sbjct: 16  PPPPPPPSTTFTSPIFPRTSSSGTNFPILAIAVIGILATAFLLVSYYIFVIKCCLNWHQI 75

Query: 66  -ITGRPRVQFEEN-ANDPSPQIHGHGLEASTIRLLPISQFKKNE---ESESTTSTNTECA 125
            I  R R   ++N     SP     GL+ S IR +P+ +FKK +     E  +  + EC+
Sbjct: 76  DIFRRRRRSSDQNPLMIYSPHEVNRGLDESAIRAIPVFKFKKRDVVAGEEDQSKNSQECS 135

Query: 126 VCLGEFEEGELIKHLPNCNHSFHSSCIDAWFRDHSSCPLCRSRV---LRFSVDCPNSDSS 164
           VCL EF+E E ++ +PNC H FH  CID W + +++CPLCR+ V     F++D  ++ SS
Sbjct: 136 VCLNEFQEDEKLRIIPNCCHVFHIDCIDIWLQGNANCPLCRTSVSCEASFTLDLISAPSS 195

[Close]

BLAST of Lag0009994 vs. ExPASy Swiss-Prot
Match: Q9LX93 (E3 ubiquitin-protein ligase RING1 OS=Arabidopsis thaliana OX=3702 GN=ATL55 PE=1 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 3.8e-18
Identity = 66/197 (33.50%), Postives = 94/197 (47.72%), Query Frame = 0


Query: 2   PSSPPPPSPVPDYSSWSPWSISTI-AIVCTVFLLLSNYRILRQLCRVLYGLIT-GRPRVQ 61
           P  PPPP P  + S   P SI  + +I+  +FL+   Y  + Q  R+    +T G    Q
Sbjct: 26  PPPPPPPPPPRELSLLLPTSICVVGSIILFLFLVFFLYLHITQQRRISAASVTPGDTNQQ 85

Query: 62  FEENANDPSP--------QIHGHGLEASTIRLLPISQFKKNEESESTTSTNTECAVCLGE 121
            +E+  +           QI   GL  S I  + +  FKK E         TEC+VCL E
Sbjct: 86  EDEDETEERDFSDFHHVWQIPTVGLHRSAINSITVVGFKKGE----GIIDGTECSVCLNE 145

Query: 122 FEEGELIKHLPNCNHSFHSSCIDAWFRDHSSCPLCRSRVLRFS------VDCPNSDSSSA 181
           FEE E ++ LP C+H+FH +CID W   H +CPLCR+ VL  +       +  +   S +
Sbjct: 146 FEEDESLRLLPKCSHAFHLNCIDTWLLSHKNCPLCRAPVLLITEPPHQETETNHQPDSES 205

Query: 182 MLETLGREDVLRERVAH 183
             +  GR+D  R R  H
Sbjct: 206 SNDLRGRQDSSRSRRNH 218

[Close]

BLAST of Lag0009994 vs. ExPASy Swiss-Prot
Match: Q7XLY8 (E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=39947 GN=Os04g0590900 PE=2 SV=2)

HSP 1 Score: 90.5 bits (223), Expect = 2.4e-17
Identity = 54/171 (31.58%), Postives = 84/171 (49.12%), Query Frame = 0


Query: 5   PPPPSPVPDYSS-------WSPWSISTIAIVCTVFLLLSNYRILRQLCRV---LYGLITG 64
           PPPP  V   S+       +SP  I+ I ++ + FLL+S Y  + + C     L G + G
Sbjct: 36  PPPPFDVAGTSADDSSGPVFSPLVIAIIGVLASAFLLVSYYTFISKYCGTVSSLRGRVFG 95

Query: 65  RPRVQFEENANDPSPQIHGH---------------GLEASTIRLLPISQFKKNEESESTT 124
                        S   HGH               GL+ + I  + + ++++ +    T 
Sbjct: 96  SSSGGAAYGGGAGSGGRHGHGQSRSHESWNVSPPSGLDETLINKITVCKYRRGDGFVHT- 155

Query: 125 STNTECAVCLGEFEEGELIKHLPNCNHSFHSSCIDAWFRDHSSCPLCRSRV 151
              T+C+VCLGEF +GE ++ LP C+H+FH  CID W + HS+CPLCR+ +
Sbjct: 156 ---TDCSVCLGEFSDGESLRLLPRCSHAFHQQCIDTWLKSHSNCPLCRANI 202

[Close]

BLAST of Lag0009994 vs. ExPASy Swiss-Prot
Match: Q9LF64 (RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 1.6e-16
Identity = 52/157 (33.12%), Postives = 82/157 (52.23%), Query Frame = 0


Query: 5   PPPPSPVPD------YSSWSPWSISTIAIVCTVFLLLSNYRILRQLCRVLYGLITGRPRV 64
           PPPPS   D       SS+SP  I+ I I+ +  +L+S Y ++ + C   +   +    +
Sbjct: 36  PPPPSFFLDDDSSSSSSSFSPLLIALIGILTSALILVSYYTLISKYCH-RHHQTSSSETL 95

Query: 65  QFEEN-----ANDPSPQIHGHGLEASTIRLLPISQFKKNEESESTTSTNTECAVCLGEFE 124
               N     ++      +G GL  S I+ + + ++K  +         ++C+VCL EFE
Sbjct: 96  NLNHNGEGFFSSTQRISTNGDGLNESMIKSITVYKYKSGDGFVD----GSDCSVCLSEFE 155

Query: 125 EGELIKHLPNCNHSFHSSCIDAWFRDHSSCPLCRSRV 151
           E E ++ LP CNH+FH  CID W + HS+CPLCR+ V
Sbjct: 156 ENESLRLLPKCNHAFHLPCIDTWLKSHSNCPLCRAFV 187

[Close]

BLAST of Lag0009994 vs. ExPASy TrEMBL
Match: A0A0A0KCA5 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G303210 PE=3 SV=1)

HSP 1 Score: 347.8 bits (891), Expect = 3.1e-92
Identity = 177/211 (83.89%), Postives = 191/211 (90.52%), Query Frame = 0


Query: 1   MPSSPPPPSPVPDYSSWSPWSISTIAIVCTVFLLLSNYRILRQLCRVLYGLITGRPRVQF 60
           MPSSPPPPSPVPDYSSWSPWSISTI +VC VFLLLSNYR+L+QLCRVL+GL TGRP VQF
Sbjct: 1   MPSSPPPPSPVPDYSSWSPWSISTIVVVCIVFLLLSNYRLLKQLCRVLHGLFTGRPTVQF 60

Query: 61  EENANDPSPQIHGHGLEASTIRLLPISQFKKNEESESTTSTNTECAVCLGEFEEGELIKH 120
           EEN N+PS QIHGHGLE S IRLLPISQFKKNEESESTTS NTECAVCLGEFEEGELIKH
Sbjct: 61  EENPNNPSLQIHGHGLETSMIRLLPISQFKKNEESESTTSFNTECAVCLGEFEEGELIKH 120

Query: 121 LPNCNHSFHSSCIDAWFRDHSSCPLCRSRVLRFSVDCPNSDSSSAMLETLGREDVLRERV 180
           LPNCNHSFHS CIDAWFR+HSSCPLCR +VL FS   P+S SSSAMLETLGRE+VLRERV
Sbjct: 121 LPNCNHSFHSPCIDAWFRNHSSCPLCRLQVLSFST--PDS-SSSAMLETLGRENVLRERV 180

Query: 181 AHYQTLRAQILQSSELRRDASPVRGSEQQNR 212
           AHYQTLRAQILQ  E RRDAS ++G++Q+NR
Sbjct: 181 AHYQTLRAQILQHPEFRRDAS-IQGTDQRNR 207

[Close]

BLAST of Lag0009994 vs. ExPASy TrEMBL
Match: A0A5A7V3S2 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold250G00390 PE=3 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 3.6e-88
Identity = 169/210 (80.48%), Postives = 184/210 (87.62%), Query Frame = 0


Query: 1   MPSSPPPPSPVPDYSSWSPWSISTIAIVCTVFLLLSNYRILRQLCRVLYGLITGRPRVQF 60
           MPSSPPPPSPVPDYSSWSPWSISTI IVC +FLLLSNYR+L+QLCRVL+ L +GRP VQF
Sbjct: 1   MPSSPPPPSPVPDYSSWSPWSISTIVIVCVIFLLLSNYRLLKQLCRVLHELFSGRPAVQF 60

Query: 61  EENANDPSPQIHGHGLEASTIRLLPISQFKKNEESESTTSTNTECAVCLGEFEEGELIKH 120
           EEN N+PS QIH HGLE S IRLLPISQFKK EESESTTS NTECAVCLGEFEEGELIK+
Sbjct: 61  EENPNNPSLQIHAHGLETSMIRLLPISQFKKKEESESTTSFNTECAVCLGEFEEGELIKN 120

Query: 121 LPNCNHSFHSSCIDAWFRDHSSCPLCRSRVLRFSVDCPNSDSSSAMLETLGREDVLRERV 180
           LPNCNHSFHSSCIDAWFRDHSSCPLCR +V  FS      DSSSAMLETLGRE++LRERV
Sbjct: 121 LPNCNHSFHSSCIDAWFRDHSSCPLCRLQVWSFS----TPDSSSAMLETLGRENILRERV 180

Query: 181 AHYQTLRAQILQSSELRRDASPVRGSEQQN 211
           AHYQTLRAQILQ  E RRD S ++GS+Q++
Sbjct: 181 AHYQTLRAQILQHPEFRRDES-IQGSDQRD 205

[Close]

BLAST of Lag0009994 vs. ExPASy TrEMBL
Match: A0A1S3BIX6 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103490579 PE=3 SV=1)

HSP 1 Score: 334.3 bits (856), Expect = 3.6e-88
Identity = 169/210 (80.48%), Postives = 184/210 (87.62%), Query Frame = 0


Query: 1   MPSSPPPPSPVPDYSSWSPWSISTIAIVCTVFLLLSNYRILRQLCRVLYGLITGRPRVQF 60
           MPSSPPPPSPVPDYSSWSPWSISTI IVC +FLLLSNYR+L+QLCRVL+ L +GRP VQF
Sbjct: 1   MPSSPPPPSPVPDYSSWSPWSISTIVIVCVIFLLLSNYRLLKQLCRVLHELFSGRPAVQF 60

Query: 61  EENANDPSPQIHGHGLEASTIRLLPISQFKKNEESESTTSTNTECAVCLGEFEEGELIKH 120
           EEN N+PS QIH HGLE S IRLLPISQFKK EESESTTS NTECAVCLGEFEEGELIK+
Sbjct: 61  EENPNNPSLQIHAHGLETSMIRLLPISQFKKKEESESTTSFNTECAVCLGEFEEGELIKN 120

Query: 121 LPNCNHSFHSSCIDAWFRDHSSCPLCRSRVLRFSVDCPNSDSSSAMLETLGREDVLRERV 180
           LPNCNHSFHSSCIDAWFRDHSSCPLCR +V  FS      DSSSAMLETLGRE++LRERV
Sbjct: 121 LPNCNHSFHSSCIDAWFRDHSSCPLCRLQVWSFS----TPDSSSAMLETLGRENILRERV 180

Query: 181 AHYQTLRAQILQSSELRRDASPVRGSEQQN 211
           AHYQTLRAQILQ  E RRD S ++GS+Q++
Sbjct: 181 AHYQTLRAQILQHPEFRRDES-IQGSDQRD 205

[Close]

BLAST of Lag0009994 vs. ExPASy TrEMBL
Match: A0A2C9W1B6 (RING-type E3 ubiquitin transferase OS=Manihot esculenta OX=3983 GN=MANES_04G030200 PE=3 SV=1)

HSP 1 Score: 181.0 bits (458), Expect = 5.1e-42
Identity = 100/200 (50.00%), Postives = 128/200 (64.00%), Query Frame = 0


Query: 1   MPSSPPPPSP--VPDYSSWSPWSISTIAIVCTVFLLLSNYRILRQLCRVLYGLITGRPRV 60
           MP SP P +P  + +YS W+ + I  +  VC+  +L S YR+LR+LC  L      R R 
Sbjct: 1   MPPSPTPEAPLRLENYSQWNAYVIGPVIAVCSSVVLFSYYRMLRRLCCTLNTQTLPRNRA 60

Query: 61  QF----EENANDPSPQIHGHGLEASTIRLLPISQFKKNEESESTTSTNTECAVCLGEFEE 120
           Q     + N  D S Q + HGLE++ +R LPISQFKK EESE   ++N ECAVCLGEFEE
Sbjct: 61  QMSRINQNNLEDSSLQNYSHGLESNIMRSLPISQFKKEEESE---ASNRECAVCLGEFEE 120

Query: 121 GELIKHLPNCNHSFHSSCIDAWFRDHSSCPLCRSRVLRFSVDCPNSDSSSAMLETLGRED 180
           GE +KHLPNC H FH +CID WF+ HS+CPLCRS V  + +    S S   +L TL RED
Sbjct: 121 GEWLKHLPNCAHVFHVACIDTWFQTHSNCPLCRSHV--YDLCHEYSISMDTLLHTLRRED 180

Query: 181 VLRERVAHYQTLRAQILQSS 195
              ER  HYQ LR++IL++S
Sbjct: 181 FFNERAEHYQNLRSEILRNS 195

[Close]

BLAST of Lag0009994 vs. ExPASy TrEMBL
Match: A0A6P6XES3 (RING-type E3 ubiquitin transferase OS=Coffea arabica OX=13443 GN=LOC113742631 PE=3 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 1.5e-41
Identity = 98/199 (49.25%), Postives = 132/199 (66.33%), Query Frame = 0


Query: 1   MPSSPPPPSPVPDYSSWSPWSISTIAIVCTVFLLLSNYRILRQLCRVLYGLIT-----GR 60
           MP SPPP S   ++S W+P  I+ + +VC +FLL S  +IL++ C   +G IT     G+
Sbjct: 49  MPPSPPPTSGF-EHSQWNPMVIALVVVVCCIFLLFSYQKILQRHCS-HFGSITNSRNQGQ 108

Query: 61  PRVQFEENANDPSPQIHGHGLEASTIRLLPISQFKKNEESESTTSTNTECAVCLGEFEEG 120
            R   +  A+DPS Q+   GL++  +  LPI+QFKKN E E T    T+CAVCLGEFEEG
Sbjct: 109 SRRLHDAVADDPSLQLQSRGLDSFVMHSLPITQFKKNNE-EETCKGITDCAVCLGEFEEG 168

Query: 121 ELIKHLPNCNHSFHSSCIDAWFRDHSSCPLCRSRVLRFSVDCPNSDSSSAMLETLGREDV 180
           E +KHLP+C+H FH SCIDAWF+ HSSCPLCRS V   ++   +S S   +LETL RE+ 
Sbjct: 169 EWLKHLPHCSHVFHVSCIDAWFQTHSSCPLCRSYVFNLTMHQEHSVSVYTLLETLRREEF 228

Query: 181 LRERVAHYQTLRAQILQSS 195
            +ER  +YQ LR+ +LQ+S
Sbjct: 229 SQERAENYQILRSHVLQTS 244

[Close]

BLAST of Lag0009994 vs. TAIR 10
Match: AT5G43420.1 (RING/U-box superfamily protein )

HSP 1 Score: 100.9 bits (250), Expect = 1.3e-21
Identity = 60/167 (35.93%), Postives = 93/167 (55.69%), Query Frame = 0


Query: 5   PPPPSPV-----PDYSSWSPWSISTIAIVCTVFLLLSNYRILRQLCRVLYGL-ITGRPRV 64
           PPPP P+        +S+   +++ I I+ T FLL+S Y  + + C   + + I GR  +
Sbjct: 17  PPPPPPIFHRASSTGTSFPILAVAVIGILATAFLLVSYYVFVIKCCLNWHRIDILGRFSL 76

Query: 65  -QFEENANDP----SPQIHGHGLEASTIRLLPISQFKK----------NEESESTTSTNT 124
            +   N  DP    SP++   GL+ S IR +PI +FKK           E  E     + 
Sbjct: 77  SRRRRNDQDPLMVYSPELRSRGLDESVIRAIPIFKFKKRYDQNDGVFTGEGEEEEEKRSQ 136

Query: 125 ECAVCLGEFEEGELIKHLPNCNHSFHSSCIDAWFRDHSSCPLCRSRV 151
           EC+VCL EF++ E ++ +PNC+H FH  CID W +++++CPLCR+RV
Sbjct: 137 ECSVCLSEFQDEEKLRIIPNCSHLFHIDCIDVWLQNNANCPLCRTRV 183

[Close]

BLAST of Lag0009994 vs. TAIR 10
Match: AT1G04360.1 (RING/U-box superfamily protein )

HSP 1 Score: 94.0 bits (232), Expect = 1.6e-19
Identity = 62/180 (34.44%), Postives = 94/180 (52.22%), Query Frame = 0


Query: 6   PPPSPVPDYSSWSP--------------WSISTIAIVCTVFLLLSNYRILRQLCRVLYGL 65
           PPP P P  +  SP               +I+ I I+ T FLL+S Y  + + C   + +
Sbjct: 16  PPPPPPPSTTFTSPIFPRTSSSGTNFPILAIAVIGILATAFLLVSYYIFVIKCCLNWHQI 75

Query: 66  -ITGRPRVQFEEN-ANDPSPQIHGHGLEASTIRLLPISQFKKNE---ESESTTSTNTECA 125
            I  R R   ++N     SP     GL+ S IR +P+ +FKK +     E  +  + EC+
Sbjct: 76  DIFRRRRRSSDQNPLMIYSPHEVNRGLDESAIRAIPVFKFKKRDVVAGEEDQSKNSQECS 135

Query: 126 VCLGEFEEGELIKHLPNCNHSFHSSCIDAWFRDHSSCPLCRSRV---LRFSVDCPNSDSS 164
           VCL EF+E E ++ +PNC H FH  CID W + +++CPLCR+ V     F++D  ++ SS
Sbjct: 136 VCLNEFQEDEKLRIIPNCCHVFHIDCIDIWLQGNANCPLCRTSVSCEASFTLDLISAPSS 195

[Close]

BLAST of Lag0009994 vs. TAIR 10
Match: AT5G10380.1 (RING/U-box superfamily protein )

HSP 1 Score: 93.2 bits (230), Expect = 2.7e-19
Identity = 66/197 (33.50%), Postives = 94/197 (47.72%), Query Frame = 0


Query: 2   PSSPPPPSPVPDYSSWSPWSISTI-AIVCTVFLLLSNYRILRQLCRVLYGLIT-GRPRVQ 61
           P  PPPP P  + S   P SI  + +I+  +FL+   Y  + Q  R+    +T G    Q
Sbjct: 26  PPPPPPPPPPRELSLLLPTSICVVGSIILFLFLVFFLYLHITQQRRISAASVTPGDTNQQ 85

Query: 62  FEENANDPSP--------QIHGHGLEASTIRLLPISQFKKNEESESTTSTNTECAVCLGE 121
            +E+  +           QI   GL  S I  + +  FKK E         TEC+VCL E
Sbjct: 86  EDEDETEERDFSDFHHVWQIPTVGLHRSAINSITVVGFKKGE----GIIDGTECSVCLNE 145

Query: 122 FEEGELIKHLPNCNHSFHSSCIDAWFRDHSSCPLCRSRVLRFS------VDCPNSDSSSA 181
           FEE E ++ LP C+H+FH +CID W   H +CPLCR+ VL  +       +  +   S +
Sbjct: 146 FEEDESLRLLPKCSHAFHLNCIDTWLLSHKNCPLCRAPVLLITEPPHQETETNHQPDSES 205

Query: 182 MLETLGREDVLRERVAH 183
             +  GR+D  R R  H
Sbjct: 206 SNDLRGRQDSSRSRRNH 218

[Close]

BLAST of Lag0009994 vs. TAIR 10
Match: AT5G17600.1 (RING/U-box superfamily protein )

HSP 1 Score: 87.8 bits (216), Expect = 1.1e-17
Identity = 52/157 (33.12%), Postives = 82/157 (52.23%), Query Frame = 0


Query: 5   PPPPSPVPD------YSSWSPWSISTIAIVCTVFLLLSNYRILRQLCRVLYGLITGRPRV 64
           PPPPS   D       SS+SP  I+ I I+ +  +L+S Y ++ + C   +   +    +
Sbjct: 36  PPPPSFFLDDDSSSSSSSFSPLLIALIGILTSALILVSYYTLISKYCH-RHHQTSSSETL 95

Query: 65  QFEEN-----ANDPSPQIHGHGLEASTIRLLPISQFKKNEESESTTSTNTECAVCLGEFE 124
               N     ++      +G GL  S I+ + + ++K  +         ++C+VCL EFE
Sbjct: 96  NLNHNGEGFFSSTQRISTNGDGLNESMIKSITVYKYKSGDGFVD----GSDCSVCLSEFE 155

Query: 125 EGELIKHLPNCNHSFHSSCIDAWFRDHSSCPLCRSRV 151
           E E ++ LP CNH+FH  CID W + HS+CPLCR+ V
Sbjct: 156 ENESLRLLPKCNHAFHLPCIDTWLKSHSNCPLCRAFV 187

[Close]

BLAST of Lag0009994 vs. TAIR 10
Match: AT4G17905.1 (RING/U-box superfamily protein )

HSP 1 Score: 87.4 bits (215), Expect = 1.5e-17
Identity = 55/164 (33.54%), Postives = 80/164 (48.78%), Query Frame = 0


Query: 4   SPPPPS-------PVPDYSSWSPWSISTIAIVCTVFLLLSNYRILRQLC----RVLYGLI 63
           SPPP S        V    + SP  I+   I  T FLL + Y ++ + C           
Sbjct: 38  SPPPISYEQFLNDGVASNPNLSPLVIAIFGIFATAFLLAAYYTLVSKYCANDTTNEAASE 97

Query: 64  TGRPRVQFEENANDPSPQIHGHGLEASTIRL-----LPISQFKKNEESESTTSTNTECAV 123
           +GR  +  + N+ +   Q     LE+ST  L       I  FK  +         T+C++
Sbjct: 98  SGRSDIILDVNSPERGDQDDPFALESSTAGLDDTLIKKIGFFKLKKHQNGFKINGTDCSI 157

Query: 124 CLGEFEEGELIKHLPNCNHSFHSSCIDAWFRDHSSCPLCRSRVL 152
           CLGEF E E ++ LP CNH+FH  CID W + HS+CPLCR++++
Sbjct: 158 CLGEFNEDESLRLLPKCNHTFHVVCIDRWLKSHSNCPLCRAKII 201

The following BLAST results are available for this feature:

BLAST of Lag0009994 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_031743630.1	6.5e-92	83.89	RING-H2 finger protein ATL52-like [Cucumis sativus] >KGN47355.1 hypothetical pro...	[more]
KAG6570731.1	5.1e-89	80.37	RING-H2 finger protein ATL16, partial [Cucurbita argyrosperma subsp. sororia] >K...	[more]
XP_008448367.1	7.4e-88	80.48	PREDICTED: RING-H2 finger protein ATL66-like [Cucumis melo] >KAA0061954.1 RING-H...	[more]
XP_023512261.1	2.8e-87	78.97	RING-H2 finger protein ATL74-like [Cucurbita pepo subsp. pepo]	[more]
XP_031247518.1	1.9e-43	48.99	RING-H2 finger protein ATL16-like [Pistacia vera]	[more]

BLAST of Lag0009994 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LSW9	1.8e-20	35.93	RING-H2 finger protein ATL16 OS=Arabidopsis thaliana OX=3702 GN=ATL16 PE=2 SV=1	[more]
P93823	2.2e-18	34.44	RING-H2 finger protein ATL1 OS=Arabidopsis thaliana OX=3702 GN=ATL1 PE=2 SV=1	[more]
Q9LX93	3.8e-18	33.50	E3 ubiquitin-protein ligase RING1 OS=Arabidopsis thaliana OX=3702 GN=ATL55 PE=1 ...	[more]
Q7XLY8	2.4e-17	31.58	E3 ubiquitin-protein ligase Os04g0590900 OS=Oryza sativa subsp. japonica OX=3994...	[more]
Q9LF64	1.6e-16	33.12	RING-H2 finger protein ATL52 OS=Arabidopsis thaliana OX=3702 GN=ATL52 PE=2 SV=1	[more]

BLAST of Lag0009994 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KCA5	3.1e-92	83.89	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_6G303210 PE...	[more]
A0A5A7V3S2	3.6e-88	80.48	RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E56...	[more]
A0A1S3BIX6	3.6e-88	80.48	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103490579 PE=3 ...	[more]
A0A2C9W1B6	5.1e-42	50.00	RING-type E3 ubiquitin transferase OS=Manihot esculenta OX=3983 GN=MANES_04G0302...	[more]
A0A6P6XES3	1.5e-41	49.25	RING-type E3 ubiquitin transferase OS=Coffea arabica OX=13443 GN=LOC113742631 PE...	[more]

BLAST of Lag0009994 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G43420.1	1.3e-21	35.93	RING/U-box superfamily protein	[more]
AT1G04360.1	1.6e-19	34.44	RING/U-box superfamily protein	[more]
AT5G10380.1	2.7e-19	33.50	RING/U-box superfamily protein	[more]
AT5G17600.1	1.1e-17	33.12	RING/U-box superfamily protein	[more]
AT4G17905.1	1.5e-17	33.54	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 105..146 e-value: 1.6E-6 score: 37.7
IPR001841	Zinc finger, RING-type	PFAM	PF13639	zf-RING_2	coord: 104..147 e-value: 8.1E-13 score: 48.5
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 105..147 score: 12.518784
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 76..155 e-value: 6.7E-22 score: 78.5
None	No IPR available	PANTHER	PTHR45676:SF107	RING/U-BOX SUPERFAMILY PROTEIN	coord: 14..162
None	No IPR available	CDD	cd16454	RING-H2_PA-TM-RING	coord: 104..147 e-value: 7.224E-17 score: 69.2529
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 104..153
IPR044675	E3 ubiquitin-protein ligase RING1-like	PANTHER	PTHR45676	RING-H2 FINGER PROTEIN ATL51-RELATED	coord: 14..162

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0009994.1	Lag0009994.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0016021	integral component of membrane