Lag0009897 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0009897
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	DNA repair protein RAD50
Location	chr9: 43201241 .. 43204696 (-)
RNA-Seq Expression	Lag0009897
Synteny	Lag0009897

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTATGTATGATTGGGGGTCTAACAAGATCACAAGATTGTGAACAACCCAATTGAAGCTAGCATATTATCAGAGGTGCAGTTTTCAAATTGATATGTCTTTTATAATGCTTATCAATTAGTAGATTGATACTTTATTAAGAAACAAAGAAGATTGGTACTTGGATCAGGGAAAGCTACTTGGAATTTTATCAGGTGACATTGATCTCTACTTTCTTAATTTTATAGGTCACGTGGGATTGCTTGATGGTGGGATCAAAGGGAATGACGTTGGCATTATCACTCCGTATAACTCCCTGGCAAGCATCATTCGTCTAGCTATCAAGATAGCCGCTGTAGAGATACATACCATTGACGAATACCAGGTTAGACTTATTCAGTTTTTATTTTTTGAAAAAAAAATAGTTTCTATTAGGTTCTTGAATTTTAGAAGGTACCAATGCACATAATAAGATGAATGCTAGAGACTAATGGTACATTTTTATAGATAAAAGAAGGTGGGAATACTTTTGCCTTCAATTGTCTATTGGTTTCACTTTCATATATTCGAGTTGAGTTGACATTGTTTTAAGAAATTAACTCATCCTTCTCTTAAGAGAAAATAATGCAATTTTAAGTTTACCTCGGTGTCTCTGACATGAATTGTCAGTAATGTTGAGTTTTAATATATCACCTACTTACTAGGTAGTAGCTATTTGATATTCTATATGCAACAAGGTGGCTAGGTAAGATTCTTAACCGTTGCTTCTCCTACATGGATTTAAACTTTTCTTTTTTGAGAATACATGGTTTTAAACTAAATTAATTTAGATTGGAATATTTGATGCCACAAAACATGTTGATGAAATTGATTGAAGGATAATGTCGGGTATAATGATTGTACTTGTGAATATAGTGAAGAATTCTATGACTATTAAGTAATCTCATTCTACTTTATCTTTCAATTGCAGATATTTTTTTTTTTGGAAACTCTATGCAATGGTCAGAGCACATCATCAAAAGGAATATACGTTCAATGGTTGAAAGGCCCGTTTCAAGAAGATTCAAAGTAGTCCATACATGTTCAGTTTCTTGTAGTGTCTACCAGGTATATTTGCCATATATTTAGCTTTTCCATTCAAACTGCAAAGAGACTTTCTGTGCTAGGTTAATTGAATTTTTGATTTCAAGGAAAAAGTTTATTATAGATGTGTGAATGATTAAATTGAAAAAATGTCACAACATATGTATGTGAATAATTAAAAAATTGGAGATGAGAAACCTTTTTTCCAACAATAGTTTGTATTTCTTTATGTTAGGCTTTCAAATTCTAGTTAAGAGTTCGTAAACCCAGAATATAAGCTCATGCGTCAAATTCTAGTTAAGAGTTCGTGAAGCTTTCATGTTTCTATTCTTTGCCTTTCCCGTGTCATTCTTGTTATTTGCTTACTCTTTAAGTCGACTACCAAGTTCTCTCTATTGTAACTGATTTGTTTCTCTTTCATTAATTTATAGAATAACTTTTTGTTTTTTTTTTTTTTGGAAACAGAGATGGTTTACACACTGAGCTTGAGAAGCTAAGGGATGAACAAAGATATATGGAAAATGATTTGTCAAACATTCAGATAAGGTGACACACTCTAAGAGAGGAAAAAGCAAAAGCAGCTAGTGCTTTGCGTGATGTTAGGAAGGCAGAAGAAGAGTTGGATCGTCTGACTGAGGAAAAAGGTCAAGTTGATTTTGATGAGAAGGTATTTTTCTTTTCTTGGTAGAAAACAAAGGAAAATATGAATAAAAAACTAAGTTCAAAGGTAAAAGAGGTTAAGGAGTTCTCTTATTAAAATATATTGCAAAAAACTTGTCCATTCAAGTGCGAGGTAAAAGAACCAAAGTATGAAAATTTACATTTTTATACTGACAGCAAGTGAAAAAATCCTGTACAGCATTAACAATTGTGCAGATCTTCTAAAAATTCTTGTTTTCCTCCAAAAGAAATGGCTCGGTGTGAAGTATAAATTGACAAATTCTCATATCTCAGGATCAGATATCTTCTAGTATATGTCAAATAGGTACATAATTTTTTTGATAGCTTGTAAGCAGACTCACTAGAAAAAAGAAAGAGGAAGAGATGACTAATAAATCTTCGTTATCTTTAATAAATAGTACTGAGTACTCAATTATGGAAAGGAGAGTATAAAACAACCTATAAAACAACATGTTCATTTGCAATTAAGTCTGAGCACTGAATTTTTCCAAAACTGAGGTCCAAAGACAACCTACTTCTGAAGGGATCAGTTTATACTAAACAAAATGATAAGGCTAAGAATAAGCGATGATGAATCAAATATAAGACATGAATTAAGAGTGGTGTGGAAAGGACTACTTGAATACGTGCATGAAACTCATTAGCAAAAGTAAATACACATGAGGACTTGAGTTTTATATTACAACAGAGTGAAGGATTAGCAGGCTTCGAATATCTGATATCTAAATTTTGATATGTGGTGAGTCATTATATGAAATTCATGTACTCTTATTTGATACATAGACAAGAGAATATGCAAGGTTAAGATTTTTTGGGCTGTGCTCCGTCACTCTGAAAGTAGTTAGAATCAATAAACTGTAGAACTAGTGATTCATAATGATAGGAGACTGGAGGTTATATTTGTTGAAGTCTATTAAAAAGTCTATCAGAGTGCTTGGAAGTTTGCCATTTAGAATTAACTCTAAAATCTAGAAAAGAAACGAATATGACTGCTTGGTGGGTCAGCTGCTTAGGTTTAAAATATTCATTCCTTTTTTCTCGTCATTGTAGGACCTTTTGTTCAGCTTCTCATTTTATGTAACAAAGTAAAATATAAAACTAAACAGGAAAATGCAAAGGGGAGTTAGTTTCTATGTAACAGTCTAACATGCCCTAGGCTAATGGCAGGACTAATTTAAGTTATAGATTAGAGGCACTTGAAATCGTTTTCTAATAAAATGCCATGGTCATGGAAAATGATTGAATGCTGAAAGTTTCTGATCAAGGATAATAATTTATATCTTTCTTTCAATTTTTTGATCTGGTTTGATCTTACCTTTCTCATGTTTCTAATCTTGTTACTATAATGATGATCTTTCCTACACTTGCAGCATTTGGCAGAAGCCCTTGTCCCTTTGTCAAAGGAGAAAGATAAATTACTTAACGACCATAATGAACTCAAAGATAAACTTAATCGTGAATATGAGGAGCTGGGTGAGAAAAAGAGGAATTTTCAGCAGGAAGTTGAGACACTTCTTAGATCAGCTTCTAAGATTAAAGAGTACGTTTGTTGAATTTTAGAGTGATTAAATGTTCTTTCGTGCCTTCAAAGCTGATGTTTAGTTTTATACTAAAAGGTATCTTGATTTCAAGAATGGTGAGAGGCTGAAAGAATTAGAGGAAAAGAAAGCACAGGCAGAATCTCAACTTCAAAGTTGTGACATTAGGAAGTAG

mRNA sequence

ATGTATATTGATACTTTATTAAGAAACAAAGAAGATTGGTACTTGGATCAGGGAAAGCTACTTGGAATTTTATCAGGTGACATTGATCTCTACTTTCTTAATTTTATAGGTCACGTGGGATTGCTTGATGGTGGGATCAAAGGGAATGACGTTGGCATTATCACTCCGTATAACTCCCTGGCAAGCATCATTCGTCTAGCTATCAAGATAGCCGCTGTAGAGATACATACCATTGACGAATACCAGGCAGAAGAAGAGTTGGATCGTCTGACTGAGGAAAAAGGTCAAGTTGATTTTGATGAGAAGCATTTGGCAGAAGCCCTTGTCCCTTTGTCAAAGGAGAAAGATAAATTACTTAACGACCATAATGAACTCAAAGATAAACTTAATCGTGAATATGAGGAGCTGGGTGAGAAAAAGAGGAATTTTCAGCAGGAAGTTGAGACACTTCTTAGATCAGCTTCTAAGATTAAAGAGTATCTTGATTTCAAGAATGGTGAGAGGCTGAAAGAATTAGAGGAAAAGAAAGCACAGGCAGAATCTCAACTTCAAAGTTGTGACATTAGGAAGTAG

Coding sequence (CDS)

ATGTATATTGATACTTTATTAAGAAACAAAGAAGATTGGTACTTGGATCAGGGAAAGCTACTTGGAATTTTATCAGGTGACATTGATCTCTACTTTCTTAATTTTATAGGTCACGTGGGATTGCTTGATGGTGGGATCAAAGGGAATGACGTTGGCATTATCACTCCGTATAACTCCCTGGCAAGCATCATTCGTCTAGCTATCAAGATAGCCGCTGTAGAGATACATACCATTGACGAATACCAGGCAGAAGAAGAGTTGGATCGTCTGACTGAGGAAAAAGGTCAAGTTGATTTTGATGAGAAGCATTTGGCAGAAGCCCTTGTCCCTTTGTCAAAGGAGAAAGATAAATTACTTAACGACCATAATGAACTCAAAGATAAACTTAATCGTGAATATGAGGAGCTGGGTGAGAAAAAGAGGAATTTTCAGCAGGAAGTTGAGACACTTCTTAGATCAGCTTCTAAGATTAAAGAGTATCTTGATTTCAAGAATGGTGAGAGGCTGAAAGAATTAGAGGAAAAGAAAGCACAGGCAGAATCTCAACTTCAAAGTTGTGACATTAGGAAGTAG

Protein sequence

MYIDTLLRNKEDWYLDQGKLLGILSGDIDLYFLNFIGHVGLLDGGIKGNDVGIITPYNSLASIIRLAIKIAAVEIHTIDEYQAEEELDRLTEEKGQVDFDEKHLAEALVPLSKEKDKLLNDHNELKDKLNREYEELGEKKRNFQQEVETLLRSASKIKEYLDFKNGERLKELEEKKAQAESQLQSCDIRK

Homology

[Close]

BLAST of Lag0009897 vs. NCBI nr
Match: XP_022146955.1 (DNA repair protein RAD50 [Momordica charantia])

HSP 1 Score: 186.0 bits (471), Expect = 2.9e-43
Identity = 100/112 (89.29%), Postives = 106/112 (94.64%), Query Frame = 0


Query: 79   DEYQAEEELDRLTEEKGQVDFDEKHLAEALVPLSKEKDKLLNDHNELKDKLNREYEELGE 138
            D  +AEEELDRLTEEK QVD DEKHLAEA+VPLSKEKDKLLND+NELK+KLNREYEELGE
Sbjct: 897  DVKKAEEELDRLTEEKSQVDLDEKHLAEAIVPLSKEKDKLLNDYNELKEKLNREYEELGE 956

Query: 139  KKRNFQQEVETLLRSASKIKEYLDFKNGERLKELEEKKAQAESQLQSCDIRK 191
            KKRNFQQEVETLLR+ASKIKEYLD K GERLKEL+EKKAQAESQLQSCDIRK
Sbjct: 957  KKRNFQQEVETLLRTASKIKEYLDLKKGERLKELQEKKAQAESQLQSCDIRK 1008

[Close]

BLAST of Lag0009897 vs. NCBI nr
Match: XP_008438322.1 (PREDICTED: DNA repair protein RAD50 [Cucumis melo])

HSP 1 Score: 181.4 bits (459), Expect = 7.2e-42
Identity = 97/112 (86.61%), Postives = 103/112 (91.96%), Query Frame = 0


Query: 79   DEYQAEEELDRLTEEKGQVDFDEKHLAEALVPLSKEKDKLLNDHNELKDKLNREYEELGE 138
            D  +AEEELDRLTEEKGQVD DEKHLAEAL+PLSKEKDKLLND+NELKDKLNREYEELG+
Sbjct: 897  DVRKAEEELDRLTEEKGQVDLDEKHLAEALIPLSKEKDKLLNDYNELKDKLNREYEELGD 956

Query: 139  KKRNFQQEVETLLRSASKIKEYLDFKNGERLKELEEKKAQAESQLQSCDIRK 191
            KKR FQQEVETLLR+ SKIKEYLD K GERLKEL+EKKAQAESQLQ CD RK
Sbjct: 957  KKRKFQQEVETLLRTTSKIKEYLDLKKGERLKELQEKKAQAESQLQGCDSRK 1008

[Close]

BLAST of Lag0009897 vs. NCBI nr
Match: XP_004133980.1 (DNA repair protein RAD50 isoform X1 [Cucumis sativus] >KGN56710.1 hypothetical protein Csa_010500 [Cucumis sativus])

HSP 1 Score: 181.4 bits (459), Expect = 7.2e-42
Identity = 97/112 (86.61%), Postives = 103/112 (91.96%), Query Frame = 0


Query: 79   DEYQAEEELDRLTEEKGQVDFDEKHLAEALVPLSKEKDKLLNDHNELKDKLNREYEELGE 138
            D  +AEEELDRLTEEKGQVD DEKHLAEAL+PLSKEKDKLLND+NELKDKLNREYEELG+
Sbjct: 897  DVRKAEEELDRLTEEKGQVDLDEKHLAEALIPLSKEKDKLLNDYNELKDKLNREYEELGD 956

Query: 139  KKRNFQQEVETLLRSASKIKEYLDFKNGERLKELEEKKAQAESQLQSCDIRK 191
            KKR FQQEVETLLR+ SKIKEYLD K GERLKEL+EKKAQAESQLQ CD RK
Sbjct: 957  KKRKFQQEVETLLRTTSKIKEYLDLKKGERLKELQEKKAQAESQLQGCDSRK 1008

[Close]

BLAST of Lag0009897 vs. NCBI nr
Match: KAA0049132.1 (DNA repair protein RAD50 [Cucumis melo var. makuwa])

HSP 1 Score: 181.4 bits (459), Expect = 7.2e-42
Identity = 97/112 (86.61%), Postives = 103/112 (91.96%), Query Frame = 0


Query: 79   DEYQAEEELDRLTEEKGQVDFDEKHLAEALVPLSKEKDKLLNDHNELKDKLNREYEELGE 138
            D  +AEEELDRLTEEKGQVD DEKHLAEAL+PLSKEKDKLLND+NELKDKLNREYEELG+
Sbjct: 897  DVRKAEEELDRLTEEKGQVDLDEKHLAEALIPLSKEKDKLLNDYNELKDKLNREYEELGD 956

Query: 139  KKRNFQQEVETLLRSASKIKEYLDFKNGERLKELEEKKAQAESQLQSCDIRK 191
            KKR FQQEVETLLR+ SKIKEYLD K GERLKEL+EKKAQAESQLQ CD RK
Sbjct: 957  KKRKFQQEVETLLRTTSKIKEYLDLKKGERLKELQEKKAQAESQLQGCDSRK 1008

[Close]

BLAST of Lag0009897 vs. NCBI nr
Match: XP_031738582.1 (DNA repair protein RAD50 isoform X2 [Cucumis sativus])

HSP 1 Score: 181.4 bits (459), Expect = 7.2e-42
Identity = 97/112 (86.61%), Postives = 103/112 (91.96%), Query Frame = 0


Query: 79   DEYQAEEELDRLTEEKGQVDFDEKHLAEALVPLSKEKDKLLNDHNELKDKLNREYEELGE 138
            D  +AEEELDRLTEEKGQVD DEKHLAEAL+PLSKEKDKLLND+NELKDKLNREYEELG+
Sbjct: 897  DVRKAEEELDRLTEEKGQVDLDEKHLAEALIPLSKEKDKLLNDYNELKDKLNREYEELGD 956

Query: 139  KKRNFQQEVETLLRSASKIKEYLDFKNGERLKELEEKKAQAESQLQSCDIRK 191
            KKR FQQEVETLLR+ SKIKEYLD K GERLKEL+EKKAQAESQLQ CD RK
Sbjct: 957  KKRKFQQEVETLLRTTSKIKEYLDLKKGERLKELQEKKAQAESQLQGCDSRK 1008

[Close]

BLAST of Lag0009897 vs. ExPASy Swiss-Prot
Match: Q9SL02 (DNA repair protein RAD50 OS=Arabidopsis thaliana OX=3702 GN=RAD50 PE=1 SV=2)

HSP 1 Score: 127.1 bits (318), Expect = 2.1e-28
Identity = 67/112 (59.82%), Postives = 91/112 (81.25%), Query Frame = 0


Query: 79   DEYQAEEELDRLTEEKGQVDFDEKHLAEALVPLSKEKDKLLNDHNELKDKLNREYEELGE 138
            D  +AEE+L+RL EEK Q+D D K+L EAL PLSKEK++LL+D+N++K + N+EYEEL E
Sbjct: 897  DVTKAEEDLERLAEEKSQLDLDVKYLTEALGPLSKEKEQLLSDYNDMKIRRNQEYEELAE 956

Query: 139  KKRNFQQEVETLLRSASKIKEYLDFKNGERLKELEEKKAQAESQLQSCDIRK 191
            KKRN+QQEVE LL+++ KI EY D K GERL +++EK+  ++SQLQSC+ RK
Sbjct: 957  KKRNYQQEVEALLKASYKINEYHDLKKGERLDDIQEKQRLSDSQLQSCEARK 1008

[Close]

BLAST of Lag0009897 vs. ExPASy Swiss-Prot
Match: A0A1P8ASY1 (DNA replication ATP-dependent helicase/nuclease JHS1 OS=Arabidopsis thaliana OX=3702 GN=JHS1 PE=2 SV=1)

HSP 1 Score: 54.3 bits (129), Expect = 1.7e-06
Identity = 25/46 (54.35%), Postives = 34/46 (73.91%), Query Frame = 0


Query: 41   LLDGGIKGNDVGIITPYNSLASIIRLAIKIAAVEIHTIDEYQAEEE 87
            L++ G+   D+GIITPYNS AS+I+ AI    VEIHTID+YQ  ++
Sbjct: 1209 LVNNGVDSKDIGIITPYNSQASLIQHAIPTTPVEIHTIDKYQGRDK 1254

The following BLAST results are available for this feature:

BLAST of Lag0009897 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022146955.1	2.9e-43	89.29	DNA repair protein RAD50 [Momordica charantia]	[more]
XP_008438322.1	7.2e-42	86.61	PREDICTED: DNA repair protein RAD50 [Cucumis melo]	[more]
XP_004133980.1	7.2e-42	86.61	DNA repair protein RAD50 isoform X1 [Cucumis sativus] >KGN56710.1 hypothetical p...	[more]
KAA0049132.1	7.2e-42	86.61	DNA repair protein RAD50 [Cucumis melo var. makuwa]	[more]
XP_031738582.1	7.2e-42	86.61	DNA repair protein RAD50 isoform X2 [Cucumis sativus]	[more]

BLAST of Lag0009897 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SL02	2.1e-28	59.82	DNA repair protein RAD50 OS=Arabidopsis thaliana OX=3702 GN=RAD50 PE=1 SV=2	[more]
A0A1P8ASY1	1.7e-06	54.35	DNA replication ATP-dependent helicase/nuclease JHS1 OS=Arabidopsis thaliana OX=...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 169..189
None	No IPR available	COILS	Coil	Coil	coord: 112..153
IPR027417	P-loop containing nucleoside triphosphate hydrolase	GENE3D	3.40.50.300		coord: 34..87 e-value: 4.0E-5 score: 25.1

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0009897.1	Lag0009897.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0070192	chromosome organization involved in meiotic cell cycle
biological_process	GO:0032508	DNA duplex unwinding
biological_process	GO:0006302	double-strand break repair
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
biological_process	GO:0016233	telomere capping
biological_process	GO:0000722	telomere maintenance via recombination
biological_process	GO:0007004	telomere maintenance via telomerase
cellular_component	GO:0000794	condensed nuclear chromosome
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0030870	Mre11 complex
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016887	ATP hydrolysis activity
molecular_function	GO:0003691	double-stranded telomeric DNA binding
molecular_function	GO:0051880	G-quadruplex DNA binding
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0043047	single-stranded telomeric DNA binding