Lag0009874 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0009874
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Ent-kaurene oxidase
Location	chr9: 43022609 .. 43023555 (+)
RNA-Seq Expression	Lag0009874
Synteny	Lag0009874

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CGATTCCGGGACTGCCTGTGCTGGGAAATTTGTTGAAGTTGAAGGAGAAAAAGCCTCACAAGACGTTTGCTCAGTGGGCCGAGACGTACGGTCCGGTTTATTCTATAAGAACCGGCATCCACTGTGGTTGTTCTCAACATCGCCGAGGTCGTCAAAGAGGTACAAACCACGGCCGTTTTCCGGAGAACTTTTGAACTCTGAATGAAAAAGTTGCCTCTGATTTTAATCGTACTCTGTTTTGGTCTTGATTCTTTAAGGCTATGGTGACGAGATATTCCTCCATGTCCAGTAGAAAGCTAAGCCAAGCTCTCACCATTTTGACTTCTGATAAATGCATGGTTGCTACGAGTGATTACAATGACTTTCACAAGTTGGTGAAGAGATATATATTGGCTAATCTCTTGGGTGTTAATGCTCAGGTGGTTTCTAATTTCTTTTTCTTCTCTTCATTTGGGTCAATTCTAATAATTTGATCTATTTAATTTTCAGAAGAGACACGGACAAAGGAGAGACACCATGATGGAGAATATTTGTAGCCAGTTAATTGTTTGCTCAGGTCAAGGATTCTCCTTCAGAATCTGTTAATTTTCGAAAGATCTTCGAAGCCGAACTCTTTCGTCTGGCGCTTAAAGAAGTGAGTTGATTCTGTTCTTTTGTTGAATACAAAACTTGATTGTATGGAAGCTAGAAAGGAGGATGAATGGCTAACATCATGGTTTCATTGGCAGACACTTGGGAAGGATGTTGAGTCGTTATATGTCGATGGACTTGGTACGACGTTGCCTAGACAATATCTGTTCAAGGTTTTAGTGATTGATCCGATGGAGGGTGCGAAAGGGAAGAAGAAGGTGAACCGAGGGGAGAAGGGAAGAAGGGAAGAAGATAAGAGAAGAGAGAGAAAGAGAAAGGAAAAAAAGAAAAAGAAAAAGAAAATGACATGTAATTAA

mRNA sequence

GATTCCGGGACTGCCTGTGCTGGGAAATTTGTTGAAGTTGAAGGAGAAAAAGCCTCACAAGACGTTTGCTCAGTGGGCCGAGACGTACGGTCCGGTTTATTCTATAAGAACCGGCATCCACTGTGGTTGTTCTCAACATCGCCGAGGTCGTCAAAGAGTAGAAAGCTAAGCCAAGCTCTCACCATTTTGACTTCTGATAAATGCATGGTTGCTACGAGTGATTACAATGACTTTCACAAGTTGGTGAAGAGATATATATTGGCTAATCTCTTGGGTGTTAATGCTCAGGTCAAGGATTCTCCTTCAGAATCTGTTAATTTTCGAAAGATCTTCGAAGCCGAACTCTTTCGTCTGGCGCTTAAAGAAACACTTGGGAAGGATGTTGAGTCGTTATATGTCGATGGACTTGGTACGACGTTGCCTAGACAATATCTGTTCAAGGTTTTAGTGATTGATCCGATGGAGGGTGCGAAAGGGAAGAAGAAGGTGAACCGAGGGGAGAAGGGAAGAAGGGAAGAAGATAAGAGAAGAGAGAGAAAGAGAAAGGAAAAAAAGAAAAAGAAAAAGAAAATGACATGTAATTAA

Coding sequence (CDS)

CGATTCCGGGACTGCCTGTGCTGGGAAATTTGTTGAAGTTGAAGGAGAAAAAGCCTCACAAGACGTTTGCTCAGTGGGCCGAGACGTACGGTCCGGTTTATTCTATAAGAACCGGCATCCACTGTGGTTGTTCTCAACATCGCCGAGGTCGTCAAAGAGTAGAAAGCTAAGCCAAGCTCTCACCATTTTGACTTCTGATAAATGCATGGTTGCTACGAGTGATTACAATGACTTTCACAAGTTGGTGAAGAGATATATATTGGCTAATCTCTTGGGTGTTAATGCTCAGGTCAAGGATTCTCCTTCAGAATCTGTTAATTTTCGAAAGATCTTCGAAGCCGAACTCTTTCGTCTGGCGCTTAAAGAAACACTTGGGAAGGATGTTGAGTCGTTATATGTCGATGGACTTGGTACGACGTTGCCTAGACAATATCTGTTCAAGGTTTTAGTGATTGATCCGATGGAGGGTGCGAAAGGGAAGAAGAAGGTGAACCGAGGGGAGAAGGGAAGAAGGGAAGAAGATAAGAGAAGAGAGAGAAAGAGAAAGGAAAAAAAGAAAAAGAAAAAGAAAATGACATGTAATTAA

Protein sequence

DSGTACAGKFVEVEGEKASQDVCSVGRDVRSGLFYKNRHPLWLFSTSPRSSKSRKLSQALTILTSDKCMVATSDYNDFHKLVKRYILANLLGVNAQVKDSPSESVNFRKIFEAELFRLALKETLGKDVESLYVDGLGTTLPRQYLFKVLVIDPMEGAKGKKKVNRGEKGRREEDKRRERKRKEKKKKKKKMTCN

Homology

[Close]

BLAST of Lag0009874 vs. NCBI nr
Match: XP_023536271.1 (ent-kaurene oxidase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 176.8 bits (447), Expect = 1.8e-40
Identity = 93/133 (69.92%), Postives = 103/133 (77.44%), Query Frame = 0


Query: 46  TSPRSSKSRKLSQALTILTSDKCMVATSDYNDFHKLVKRYILANLLGVNAQ--------- 105
           T   S  SRKLS+ALTILTSDKCMVA SDYNDFHK+VK+YILAN+LG NAQ         
Sbjct: 114 TRHASMSSRKLSKALTILTSDKCMVAMSDYNDFHKMVKKYILANVLGANAQKRHRQRRDS 173

Query: 106 ------------VKDSPSESVNFRKIFEAELFRLALKETLGKDVESLYVDGLGTTLPRQY 158
                       VKDSPSESVNFRKIFE+ELFRLALKET+GKD+ES+YVDGL TTLPR+ 
Sbjct: 174 MIDNISSQLFAHVKDSPSESVNFRKIFESELFRLALKETVGKDIESIYVDGLSTTLPRED 233

[Close]

BLAST of Lag0009874 vs. NCBI nr
Match: XP_038898390.1 (ent-kaurene oxidase [Benincasa hispida])

HSP 1 Score: 176.4 bits (446), Expect = 2.4e-40
Identity = 92/129 (71.32%), Postives = 102/129 (79.07%), Query Frame = 0


Query: 50  SSKSRKLSQALTILTSDKCMVATSDYNDFHKLVKRYILANLLGVNAQ------------- 109
           S  SRKLS+ALTILT+DKCMVA SDYNDFHK+VKRYILAN+LG NAQ             
Sbjct: 118 SISSRKLSKALTILTADKCMVAMSDYNDFHKMVKRYILANMLGANAQKKHRQRRDTMIEN 177

Query: 110 --------VKDSPSESVNFRKIFEAELFRLALKETLGKDVESLYVDGLGTTLPRQYLFKV 158
                   VK+ PSESVNFRKIFE+ELFRLALKETLGKD+ES+YVDGLGTTLPR+ LF+V
Sbjct: 178 ISRELFAHVKEFPSESVNFRKIFESELFRLALKETLGKDIESIYVDGLGTTLPREDLFRV 237

[Close]

BLAST of Lag0009874 vs. NCBI nr
Match: KAG6591351.1 (Ent-kaurene oxidase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 172.2 bits (435), Expect = 4.5e-39
Identity = 92/129 (71.32%), Postives = 102/129 (79.07%), Query Frame = 0


Query: 50  SSKSRKLSQALTILTSDKCMVATSDYNDFHKLVKRYILANLLGVNAQ------------- 109
           S  SRKLS+ALTILTSDKCMVA SDYNDFHK+VK+YILAN+LG NAQ             
Sbjct: 118 SMSSRKLSKALTILTSDKCMVAMSDYNDFHKMVKKYILANVLGANAQKRHRQRRDTMIDN 177

Query: 110 --------VKDSPSESVNFRKIFEAELFRLALKETLGKDVESLYVDGLGTTLPRQYLFKV 158
                   VKDSPSESVNFR+IFE+ELFRLALKETLGKD+ES+YVDGLG TLPR+ LF+V
Sbjct: 178 ISSQLFAHVKDSPSESVNFRRIFESELFRLALKETLGKDIESIYVDGLG-TLPREDLFRV 237

[Close]

BLAST of Lag0009874 vs. NCBI nr
Match: KAG7024228.1 (Ent-kaurene oxidase, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 172.2 bits (435), Expect = 4.5e-39
Identity = 92/129 (71.32%), Postives = 102/129 (79.07%), Query Frame = 0


Query: 50  SSKSRKLSQALTILTSDKCMVATSDYNDFHKLVKRYILANLLGVNAQ------------- 109
           S  SRKLS+ALTILTSDKCMVA SDYNDFHK+VK+YILAN+LG NAQ             
Sbjct: 118 SMSSRKLSKALTILTSDKCMVAMSDYNDFHKMVKKYILANVLGANAQKRHRQRRDTMIDN 177

Query: 110 --------VKDSPSESVNFRKIFEAELFRLALKETLGKDVESLYVDGLGTTLPRQYLFKV 158
                   VKDSPSESVNFR+IFE+ELFRLALKETLGKD+ES+YVDGLG TLPR+ LF+V
Sbjct: 178 ISSQLFAHVKDSPSESVNFRRIFESELFRLALKETLGKDIESIYVDGLG-TLPREDLFRV 237

[Close]

BLAST of Lag0009874 vs. NCBI nr
Match: XP_022976063.1 (ent-kaurene oxidase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 172.2 bits (435), Expect = 4.5e-39
Identity = 91/129 (70.54%), Postives = 100/129 (77.52%), Query Frame = 0


Query: 50  SSKSRKLSQALTILTSDKCMVATSDYNDFHKLVKRYILANLLGVNAQ------------- 109
           S   RKLS+ALTILTSDKCMVA SDYNDFHK+VK+YILAN+LG NAQ             
Sbjct: 118 SMSGRKLSKALTILTSDKCMVAMSDYNDFHKMVKKYILANVLGANAQKRHRQRRDSMIDN 177

Query: 110 --------VKDSPSESVNFRKIFEAELFRLALKETLGKDVESLYVDGLGTTLPRQYLFKV 158
                   VKDSPSESVNFRKIFE+ELF LALKETLGKD+ES+YVDGLGTTLPR+ LF+V
Sbjct: 178 ISSQLFAHVKDSPSESVNFRKIFESELFLLALKETLGKDIESIYVDGLGTTLPREDLFRV 237

[Close]

BLAST of Lag0009874 vs. ExPASy Swiss-Prot
Match: A0A0G2RKY1 (Ent-kaurene oxidase OS=Salvia miltiorrhiza OX=226208 GN=KO PE=1 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 3.3e-29
Identity = 69/133 (51.88%), Postives = 90/133 (67.67%), Query Frame = 0


Query: 46  TSPRSSKSRKLSQALTILTSDKCMVATSDYNDFHKLVKRYILANLLGVNAQ--------- 105
           T   S  +RKLS+ALTILTSDK +VA SDYN+F+K  KR++L + LG  AQ         
Sbjct: 107 TKYSSISTRKLSKALTILTSDKSIVAMSDYNEFYKAAKRHLLTSTLGPTAQKRHRVHRNL 166

Query: 106 ------------VKDSPSESVNFRKIFEAELFRLALKETLGKDVESLYVDGLGTTLPRQY 158
                        K  PSE+VNFRKIF++ELF L++K+ +G+DVES+YV+ L TTL RQ 
Sbjct: 167 MINNICDQFLAHAKMYPSEAVNFRKIFQSELFGLSMKQAIGEDVESIYVEDLDTTLSRQE 226

[Close]

BLAST of Lag0009874 vs. ExPASy Swiss-Prot
Match: Q93ZB2 (Ent-kaurene oxidase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=KO PE=1 SV=2)

HSP 1 Score: 129.0 bits (323), Expect = 5.7e-29
Identity = 72/129 (55.81%), Postives = 82/129 (63.57%), Query Frame = 0


Query: 50  SSKSRKLSQALTILTSDKCMVATSDYNDFHKLVKRYILANLLGVNAQ------------- 109
           S  +RKLS ALT+LT DK MVATSDY+DFHKLVKR +L  LLG NAQ             
Sbjct: 107 SISTRKLSNALTVLTCDKSMVATSDYDDFHKLVKRCLLNGLLGANAQKRKRHYRDALIEN 166

Query: 110 --------VKDSPSESVNFRKIFEAELFRLALKETLGKDVESLYVDGLGTTLPRQYLFKV 158
                    +D P E VNFR IFE ELF +ALK+  GKDVES+YV  LG TL +  +FKV
Sbjct: 167 VSSKLHAHARDHPQEPVNFRAIFEHELFGVALKQAFGKDVESIYVKELGVTLSKDEIFKV 226

[Close]

BLAST of Lag0009874 vs. ExPASy Swiss-Prot
Match: Q5Z5R4 (Ent-kaurene oxidase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A6 PE=1 SV=1)

HSP 1 Score: 106.3 bits (264), Expect = 4.0e-22
Identity = 55/126 (43.65%), Postives = 79/126 (62.70%), Query Frame = 0


Query: 50  SSKSRKLSQALTILTSDKCMVATSDYNDFHKLVKRYILANLLGVNAQ------------- 109
           S  +RKLS+ALT+LT DK MVATSDY DFHK+VKRY+++++LG +AQ             
Sbjct: 104 SISTRKLSKALTVLTRDKSMVATSDYCDFHKMVKRYVMSSMLGTSAQKQFRDIRDMMIHN 163

Query: 110 --------VKDSPSESVNFRKIFEAELFRLALKETLGKDVESLYVDGLGTTLPRQYLFKV 155
                   VKD P   + FR +F+ ELFRL++ ++LG+DV S+YVD  G  + ++ ++  
Sbjct: 164 MLSTFHKLVKDDPHAPLIFRDVFKDELFRLSMIQSLGEDVSSVYVDEFGRDISKEEIYNA 223

[Close]

BLAST of Lag0009874 vs. ExPASy Swiss-Prot
Match: C7J3A2 (Ent-kaurene oxidase-like protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A7 PE=5 SV=1)

HSP 1 Score: 105.9 bits (263), Expect = 5.2e-22
Identity = 55/126 (43.65%), Postives = 78/126 (61.90%), Query Frame = 0


Query: 50  SSKSRKLSQALTILTSDKCMVATSDYNDFHKLVKRYILANLLGVNAQ------------- 109
           S  +RKLS+ALT+LT DK MVATSDY DFHK+VKRY++++ LG +AQ             
Sbjct: 115 SISTRKLSKALTVLTRDKSMVATSDYGDFHKMVKRYVMSSTLGTSAQKKFRDTRDMMINN 174

Query: 110 --------VKDSPSESVNFRKIFEAELFRLALKETLGKDVESLYVDGLGTTLPRQYLFKV 155
                   VKD P   + FR +F+ ELFRL++ ++LG+DV S+YVD  G  + ++ ++  
Sbjct: 175 MLSTFHKLVKDDPHVPLIFRDVFKDELFRLSMIQSLGEDVSSVYVDEFGRDISKEEIYNA 234

[Close]

BLAST of Lag0009874 vs. ExPASy Swiss-Prot
Match: Q5Z5R7 (Ent-kaurene oxidase-like 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A19 PE=2 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 4.8e-20
Identity = 53/126 (42.06%), Postives = 76/126 (60.32%), Query Frame = 0


Query: 50  SSKSRKLSQALTILTSDKCMVATSDYNDFHKLVKRYILANLLGVNAQ------------- 109
           S  +RKLS+ALT+L+ DK MVATSD  DFHK+ KRYI+ ++LG +AQ             
Sbjct: 103 SISTRKLSKALTVLSHDKSMVATSDSGDFHKMGKRYIMLSMLGTSAQKQFRDTRDMIINN 162

Query: 110 --------VKDSPSESVNFRKIFEAELFRLALKETLGKDVESLYVDGLGTTLPRQYLFKV 155
                   VKD P   + FR +F+ ELFRL++ ++LG+DV S+YVD  G  + ++ ++  
Sbjct: 163 MLSTFHQLVKDDPHAPLIFRDVFKDELFRLSMIQSLGEDVSSVYVDEFGRDISKEEIYNA 222

The following BLAST results are available for this feature:

BLAST of Lag0009874 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023536271.1	1.8e-40	69.92	ent-kaurene oxidase, chloroplastic-like [Cucurbita pepo subsp. pepo]	[more]
XP_038898390.1	2.4e-40	71.32	ent-kaurene oxidase [Benincasa hispida]	[more]
KAG6591351.1	4.5e-39	71.32	Ent-kaurene oxidase, chloroplastic, partial [Cucurbita argyrosperma subsp. soror...	[more]
KAG7024228.1	4.5e-39	71.32	Ent-kaurene oxidase, chloroplastic, partial [Cucurbita argyrosperma subsp. argyr...	[more]
XP_022976063.1	4.5e-39	70.54	ent-kaurene oxidase, chloroplastic-like [Cucurbita maxima]	[more]

BLAST of Lag0009874 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A0G2RKY1	3.3e-29	51.88	Ent-kaurene oxidase OS=Salvia miltiorrhiza OX=226208 GN=KO PE=1 SV=1	[more]
Q93ZB2	5.7e-29	55.81	Ent-kaurene oxidase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=KO PE=1 SV...	[more]
Q5Z5R4	4.0e-22	43.65	Ent-kaurene oxidase 2 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A6 PE=1 ...	[more]
C7J3A2	5.2e-22	43.65	Ent-kaurene oxidase-like protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=C...	[more]
Q5Z5R7	4.8e-20	42.06	Ent-kaurene oxidase-like 3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP701A19...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 170..190
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 158..194
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 158..179
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 180..194
IPR044225	Ent-kaurene oxidase, chloroplastic	PANTHER	PTHR47283	ENT-KAURENE OXIDASE, CHLOROPLASTIC	coord: 95..157 coord: 50..98

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0009874.1	Lag0009874.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010241	ent-kaurene oxidation to kaurenoic acid
biological_process	GO:0009686	gibberellin biosynthetic process
cellular_component	GO:0009707	chloroplast outer membrane
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0052615	ent-kaurene oxidase activity
molecular_function	GO:0020037	heme binding
molecular_function	GO:0005506	iron ion binding