Lag0009743 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0009743
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionWRKY domain-containing protein
Locationchr9: 41926862 .. 41928864 (+)
RNA-Seq ExpressionLag0009743
SyntenyLag0009743
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCAAATGAAGATCATCAGAAGGATTCTTGTTACCAAATATTTGATCCTTTCACTCACAATTTCCATCATCACAAACCCTTTGAAGCTCCTCTTTTAAGTCCCTATGATGCCTTTGATCCCTCTTATGTGGGGTTCTTCTCTGATTTCTTACATGCCTCAGCTGATTATGACTACAACAACATCCTCTCCACTGGCCTCGAGATGTCGTGCTCGTCGTCCGAAGTTATTTCTCCAATCGACGATGCTTCGAAGAAGTCTATGGGCGTCGGAGAGTCCGTGGTGACCGGTGAGCACCCGTCGACGCCGATTTCCTCGACGGCGTCTTCGTCCGATGAGGCTGCGCCGGGTGGTGGTGATTCTGCCAAGAGTGGGGAAGAAGTGAAAGAGTTTGAAGATAAAAGTGGAGAAAATTGCAAGAAAGGGTAGGTCTTCTCTCTACTCCTCCCTTCCATAGGTTAGGAGCTTAGGCTTAGATGGTAATGCCTAAAAAAAAATTAGGGTTTGCTAAAGGTTCTTAATTAATTTATGGTTTTATTCAAGTCTAAACTCAACCCATTTCTTATTCTTAGAAGGAAATTTACAAGAAAACTCAAAAGGGTTAACACTATTTGTGATTTTTGAGCTAAATATGCAATAAAAATCAATGTGGAATATGTATGTGGTTAATATTTGTTCAAAAAAATTGACAAAACAAGTTTGGTTTAATTTGTTTGGTACAGGAATGGACGAAAAATAAAGAAAGAGAAACGAGAGAAAGGGCCACGATTTGCGTTCTTGACCAAGACTGAAATTGATCATCTTGAAGATGGATATAGGTGGAGAAAATATGGTCAGAAAGCAGTCAAAAACAGCCCTTTTCCAAGGTTTCATTTCATTTTTCTTAAATTTCTCCACTTTTTTTTTTTTCCTTATCAATTATGCATCTCAATATTTAAAGCCAATTTGGAAATGGGTTTTTTTTGGATTTAGTTTATCACATCTAAATTGAATCAAAGTTCAAATCTTTGTGATTGTATATTTTATATTCCTCTCTCCCATTTCTGTACAAATTAGGTATAAAAAAAAAAAAAAAAAAAGAGAAATTTGTTAGTCAATTATTTCATACCCTGAAAATTCTTATGTATATTGTATTTGAATTGACATAAACCAAGCTCAGATTATAATAACCCATAAAGCTGCAACATATAATTTGTGTACTGTATAGAACATGAACACTAATTGATTGTAACCCTAGAAATATTTTTATGACTTTTATCATTTCCTCAAAAGGGTTTGTGATTCTTCTTCTCCTTTCCAAATGTGATTTCAAAATTAAAGTTGGTCGCTTCCCATATCGCCTTGAATTGGAAAGCTTTCATGTAGCGCTCTTTTGCATGTTTCTCACATACTCCTCAATAAAGTATATTCTCTTTTCTAAAAAAGATTATAAGTTTGTTAGCAATTACCAACCTAAACATAGTTCAATTGACTAAGACATATATCTCCGACCAAAAGGTCGATATGGCACATATATTGTTGAACTATATTAAATAAACATTGGGTTCCATCTTAAAACCAATTGACAATGAAAAGATTAATTCATATATCTTATAAATTGTGAGTTTTCATTCATCTTTTCAATGTGGAATCCTCAACGAACTTCATTACAGATGCATCTTTTTTTGTTTTGTTACAGAAGTTATTACAAATGCACAACTCAAAATTGCAGTGTGAAAAAACGAATAGAAAGATCAACCGAAGATCCATGTTTTGTTGTGACAACGTACGAAGGGAAACACAACCACCATTGTCCAATTACACTTCGAGGGCAGAATGCCGCCGGAGTGTTGCCGGCGTCGGCTTCACCGCCGTTGCTTCCCGGATTAGCTCCGGCGAACTTCTTCTCCGACGAGGTGTATGAAGGGGTTGATCTGCAGCAACAATATTATCAGGTTCCAGATTATGGGCTGTTGCAAGATTTGATTAATCCATTATCATTCAATCGAAAGCAAGAGCCATGA

mRNA sequence

ATGTCAAATGAAGATCATCAGAAGGATTCTTGTTACCAAATATTTGATCCTTTCACTCACAATTTCCATCATCACAAACCCTTTGAAGCTCCTCTTTTAAGTCCCTATGATGCCTTTGATCCCTCTTATGTGGGGTTCTTCTCTGATTTCTTACATGCCTCAGCTGATTATGACTACAACAACATCCTCTCCACTGGCCTCGAGATGTCGTGCTCGTCGTCCGAAGTTATTTCTCCAATCGACGATGCTTCGAAGAAGTCTATGGGCGTCGGAGAGTCCGTGGTGACCGGTGAGCACCCGTCGACGCCGATTTCCTCGACGGCGTCTTCGTCCGATGAGGCTGCGCCGGGTGGTGGTGATTCTGCCAAGAGTGGGGAAGAAGTGAAAGAGTTTGAAGATAAAAGTGGAGAAAATTGCAAGAAAGGGAATGGACGAAAAATAAAGAAAGAGAAACGAGAGAAAGGGCCACGATTTGCGTTCTTGACCAAGACTGAAATTGATCATCTTGAAGATGGATATAGGTGGAGAAAATATGGTCAGAAAGCAGTCAAAAACAGCCCTTTTCCAAGAAGTTATTACAAATGCACAACTCAAAATTGCAGTGTGAAAAAACGAATAGAAAGATCAACCGAAGATCCATGTTTTGTTGTGACAACGTACGAAGGGAAACACAACCACCATTGTCCAATTACACTTCGAGGGCAGAATGCCGCCGGAGTGTTGCCGGCGTCGGCTTCACCGCCGTTGCTTCCCGGATTAGCTCCGGCGAACTTCTTCTCCGACGAGGTGTATGAAGGGGTTGATCTGCAGCAACAATATTATCAGGTTCCAGATTATGGGCTGTTGCAAGATTTGATTAATCCATTATCATTCAATCGAAAGCAAGAGCCATGA

Coding sequence (CDS)

ATGTCAAATGAAGATCATCAGAAGGATTCTTGTTACCAAATATTTGATCCTTTCACTCACAATTTCCATCATCACAAACCCTTTGAAGCTCCTCTTTTAAGTCCCTATGATGCCTTTGATCCCTCTTATGTGGGGTTCTTCTCTGATTTCTTACATGCCTCAGCTGATTATGACTACAACAACATCCTCTCCACTGGCCTCGAGATGTCGTGCTCGTCGTCCGAAGTTATTTCTCCAATCGACGATGCTTCGAAGAAGTCTATGGGCGTCGGAGAGTCCGTGGTGACCGGTGAGCACCCGTCGACGCCGATTTCCTCGACGGCGTCTTCGTCCGATGAGGCTGCGCCGGGTGGTGGTGATTCTGCCAAGAGTGGGGAAGAAGTGAAAGAGTTTGAAGATAAAAGTGGAGAAAATTGCAAGAAAGGGAATGGACGAAAAATAAAGAAAGAGAAACGAGAGAAAGGGCCACGATTTGCGTTCTTGACCAAGACTGAAATTGATCATCTTGAAGATGGATATAGGTGGAGAAAATATGGTCAGAAAGCAGTCAAAAACAGCCCTTTTCCAAGAAGTTATTACAAATGCACAACTCAAAATTGCAGTGTGAAAAAACGAATAGAAAGATCAACCGAAGATCCATGTTTTGTTGTGACAACGTACGAAGGGAAACACAACCACCATTGTCCAATTACACTTCGAGGGCAGAATGCCGCCGGAGTGTTGCCGGCGTCGGCTTCACCGCCGTTGCTTCCCGGATTAGCTCCGGCGAACTTCTTCTCCGACGAGGTGTATGAAGGGGTTGATCTGCAGCAACAATATTATCAGGTTCCAGATTATGGGCTGTTGCAAGATTTGATTAATCCATTATCATTCAATCGAAAGCAAGAGCCATGA

Protein sequence

MSNEDHQKDSCYQIFDPFTHNFHHHKPFEAPLLSPYDAFDPSYVGFFSDFLHASADYDYNNILSTGLEMSCSSSEVISPIDDASKKSMGVGESVVTGEHPSTPISSTASSSDEAAPGGGDSAKSGEEVKEFEDKSGENCKKGNGRKIKKEKREKGPRFAFLTKTEIDHLEDGYRWRKYGQKAVKNSPFPRSYYKCTTQNCSVKKRIERSTEDPCFVVTTYEGKHNHHCPITLRGQNAAGVLPASASPPLLPGLAPANFFSDEVYEGVDLQQQYYQVPDYGLLQDLINPLSFNRKQEP
Homology
BLAST of Lag0009743 vs. NCBI nr
Match: XP_038902581.1 (probable WRKY transcription factor 56 [Benincasa hispida])

HSP 1 Score: 419.9 bits (1078), Expect = 1.9e-113
Identity = 231/309 (74.76%), Postives = 255/309 (82.52%), Query Frame = 0

Query: 1   MSNEDH-----QKDSCYQIFDPFTHNFHHHKPFEA-PLLSP-YDAFD--PSYVGFFSDFL 60
           MSNEDH     +++  Y  FDPFTHNFH HKPFEA PL SP Y+AFD  PS VGF+SDFL
Sbjct: 1   MSNEDHHHHEEEENPYYPNFDPFTHNFHPHKPFEAPPLTSPYYEAFDIIPSSVGFYSDFL 60

Query: 61  HASADYDYNNILSTGLEMSCSSSEVISPIDDASKKSMGV-GESVVTGEHPSTP-ISSTAS 120
           H SA+Y YNN L   LEMSCSSSEVISPIDDASKKS+G+  +SVVTGEHPSTP  SST  
Sbjct: 61  HVSAEYGYNNNL---LEMSCSSSEVISPIDDASKKSLGLRDQSVVTGEHPSTPNCSSTTC 120

Query: 121 SSDEAAPGGGDSAKSGEEVKEFEDKSGENCKKGNGRKIKKEKREKGPRFAFLTKTEIDHL 180
           SSDEAA GGG+S+KSG EVK F+DK+GEN KK +G K KKEKREKGPRFAF+TKTEID+L
Sbjct: 121 SSDEAAAGGGESSKSG-EVKGFDDKNGENSKKVDGGK-KKEKREKGPRFAFMTKTEIDNL 180

Query: 181 EDGYRWRKYGQKAVKNSPFPRSYYKCTTQNCSVKKRIERSTEDPCFVVTTYEGKHNHHCP 240
           EDGYRWRKYGQKAVKNSPFPRSYYKCT+QNCSVKKR+ERS+EDPCFV+TTYEGKHNH+CP
Sbjct: 181 EDGYRWRKYGQKAVKNSPFPRSYYKCTSQNCSVKKRVERSSEDPCFVITTYEGKHNHYCP 240

Query: 241 ITLRGQNAAGVLPASASPPLLPGLAPANFFSDEVYEGVDLQQQYYQVPD-YGLLQDLINP 298
           I LRG NA GVLP S SPP LPGLAPANF S E YE ++ QQ Y QVPD YGLLQDL+NP
Sbjct: 241 IILRGHNATGVLPPSVSPPYLPGLAPANFLSGEFYENLNQQQYYDQVPDQYGLLQDLVNP 300

BLAST of Lag0009743 vs. NCBI nr
Match: XP_011656990.1 (probable WRKY transcription factor 12 isoform X1 [Cucumis sativus] >KGN46828.1 hypothetical protein Csa_020630 [Cucumis sativus])

HSP 1 Score: 365.9 bits (938), Expect = 3.2e-97
Identity = 211/311 (67.85%), Postives = 234/311 (75.24%), Query Frame = 0

Query: 1   MSNE--DHQKDS---CYQIFDPFTHNFHHHKPFEA--PLLSP-YDAFD--PSYVGFFSDF 60
           MSNE  DH+K+     Y  FDPFTHNFHH KPFE   PL +P Y+AFD  PS VGF+SDF
Sbjct: 1   MSNEGHDHEKEEDHPYYPQFDPFTHNFHHQKPFEVPPPLTNPYYEAFDIAPSSVGFYSDF 60

Query: 61  LHASADYDYNNILSTGLEMSCSSSEVISPIDDASKKSMGVG---ESVVTGEHPSTP-ISS 120
            HAS+ YDY+NI ST +EMSCSSSEVIS +DDA KKS  +G    SVVTGEHPSTP  SS
Sbjct: 61  SHASSQYDYHNIFSTPIEMSCSSSEVISSVDDALKKSSSLGRDLSSVVTGEHPSTPNCSS 120

Query: 121 TASSSDEAAPGGGDSAKSGEEVKEFEDKSGENCKKGNGRKIKKEKREKGPRFAFLTKTEI 180
           T  SSDE   GGGDS+KSG EVK F+DK GEN KK +  K KKEKREKGPRFAFLTKTEI
Sbjct: 121 TTCSSDEVVAGGGDSSKSG-EVKGFDDKKGENSKKVDEGK-KKEKREKGPRFAFLTKTEI 180

Query: 181 DHLEDGYRWRKYGQKAVKNSPFPRSYYKCTTQNCSVKKRIERSTEDPCFVVTTYEGKHNH 240
           D+LEDGYRWRKYGQKAVKNSPFPRSYYKCT+QNCSVKKR+ERS+EDP FV+TTYEGKHNH
Sbjct: 181 DNLEDGYRWRKYGQKAVKNSPFPRSYYKCTSQNCSVKKRVERSSEDPSFVITTYEGKHNH 240

Query: 241 HCPITLRGQNAAGVLPASASPPLLPGLAPANFFSDEVY-EGVDLQQQYYQVPDYGLLQDL 297
           +CPITLRG N  GVLP S +PPL P     NFFS E + E +  Q QY      GL QD 
Sbjct: 241 YCPITLRGHNPTGVLPPSVTPPLFP--PSPNFFSAESFCENLHQQYQY-----GGLFQDF 300

BLAST of Lag0009743 vs. NCBI nr
Match: XP_008456349.1 (PREDICTED: probable WRKY transcription factor 12 [Cucumis melo])

HSP 1 Score: 364.0 bits (933), Expect = 1.2e-96
Identity = 211/310 (68.06%), Postives = 232/310 (74.84%), Query Frame = 0

Query: 1   MSNE--DHQK---DSCYQIFDPFTHNFHHHKPFEA--PLLSPY-DAFD--PSYVGFFSDF 60
           MSNE  DH+K   D  Y  FDPFTHNFHH KPFE   PL +PY +AFD  P  VGF+SDF
Sbjct: 1   MSNEGHDHEKEEDDPYYPQFDPFTHNFHHQKPFEVPPPLTNPYSEAFDIAPYNVGFYSDF 60

Query: 61  LHASADYDYNNILSTGLEMSCSSSEVISPIDDASKKSMGVG---ESVVTGEHPSTP-ISS 120
            HAS+ YDY+NI ST  EMSCSSSEVIS +DDA KKS  +G    SVVTGEHP TP  SS
Sbjct: 61  SHASSQYDYHNIFSTPFEMSCSSSEVISSVDDALKKSSSLGRDLSSVVTGEHPLTPNCSS 120

Query: 121 TASSSDEAAPGGGDSAKSGEEVKEFEDKSGENCKKGNGRKIKKEKREKGPRFAFLTKTEI 180
           T  SSDE   GGGDS+KSG EVK F+DK GEN KK +  K KKEKREKGPRFAFLTKTEI
Sbjct: 121 TTCSSDEVVAGGGDSSKSG-EVKGFDDKKGENSKKVDEGK-KKEKREKGPRFAFLTKTEI 180

Query: 181 DHLEDGYRWRKYGQKAVKNSPFPRSYYKCTTQNCSVKKRIERSTEDPCFVVTTYEGKHNH 240
           D+LEDGYRWRKYGQKAVKNSPFPRSYYKCT+QNCSVKKR+ERS+EDP FV+TTYEGKHNH
Sbjct: 181 DNLEDGYRWRKYGQKAVKNSPFPRSYYKCTSQNCSVKKRVERSSEDPGFVITTYEGKHNH 240

Query: 241 HCPITLRGQNAAGVLPASASPPLLPGLAPANFFSDEVYEGVDLQQQYYQVPDYGLLQDLI 297
           +CPITLRG N  GVLP S +PPL P     NFFS E +   +L QQY     YGL QD I
Sbjct: 241 YCPITLRGHNPTGVLPPSVTPPLFP--PSPNFFSAESF-CENLHQQY----QYGLFQDFI 300

BLAST of Lag0009743 vs. NCBI nr
Match: XP_022943476.1 (probable WRKY transcription factor 28 isoform X1 [Cucurbita moschata])

HSP 1 Score: 346.3 bits (887), Expect = 2.7e-91
Identity = 193/296 (65.20%), Postives = 218/296 (73.65%), Query Frame = 0

Query: 2   SNEDHQKDSCYQIFDPFTHNFHHHKPFEAPLLSPYDAFDPSYVGFFSDFLHASADYDYNN 61
           +NEDH+K       DP+  NF              +AFDPS VGF+SDFL     YDYNN
Sbjct: 3   NNEDHEK-------DPYYPNF--------------EAFDPSNVGFYSDFL-----YDYNN 62

Query: 62  ILSTGLEMSCSSSEVISPIDDASKKSMGVGESVVTGEHPSTPISSTASSSDEAAPGGGDS 121
            LST +++SCSSSE ISPI+DA KKS+G+GESV TGEHPSTP SST  SS EAA GG DS
Sbjct: 63  FLSTDIDISCSSSEAISPINDALKKSLGLGESVATGEHPSTPNSSTTCSSVEAAAGGRDS 122

Query: 122 AKSGEEVKEFEDKSGENCKKGNGRKIKKEKREKGPRFAFLTKTEIDHLEDGYRWRKYGQK 181
           AKSGE              K +GRK KKEKREKGPRFAFLTK+EID+LEDGYRWRKYGQK
Sbjct: 123 AKSGE-------------VKVDGRK-KKEKREKGPRFAFLTKSEIDNLEDGYRWRKYGQK 182

Query: 182 AVKNSPFPRSYYKCTTQNCSVKKRIERSTEDPCFVVTTYEGKHNHHCPITLRGQNAAGVL 241
           AVKNSPFPRSYYKCT+QNCSVKKR+ERS+EDPCFVVTTYEGKHNH+CPITLRG N AGV 
Sbjct: 183 AVKNSPFPRSYYKCTSQNCSVKKRVERSSEDPCFVVTTYEGKHNHYCPITLRGHNVAGVY 242

Query: 242 PASASPPLLPGLAPANFFSDEVYEGVDLQQQYYQVPDYGLLQDLINPLSFNRKQEP 298
           P SASP L+    PA   + E Y GVD+QQQYY+VP YG+L+DL+NP     KQ P
Sbjct: 243 PPSASPSLI----PARILAGEFYGGVDVQQQYYEVPGYGVLEDLVNP-----KQNP 249

BLAST of Lag0009743 vs. NCBI nr
Match: XP_023512278.1 (probable WRKY transcription factor 48 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 345.5 bits (885), Expect = 4.5e-91
Identity = 193/296 (65.20%), Postives = 217/296 (73.31%), Query Frame = 0

Query: 2   SNEDHQKDSCYQIFDPFTHNFHHHKPFEAPLLSPYDAFDPSYVGFFSDFLHASADYDYNN 61
           +NEDH+K       DP+  NF              +AFDPS VGF+SDFL     YDYNN
Sbjct: 3   NNEDHEK-------DPYYPNF--------------EAFDPSNVGFYSDFL-----YDYNN 62

Query: 62  ILSTGLEMSCSSSEVISPIDDASKKSMGVGESVVTGEHPSTPISSTASSSDEAAPGGGDS 121
            LST +++SCSSSE ISPI+DA KKS+G+GESV  GEHPSTP SST  SS EAA GG DS
Sbjct: 63  FLSTDIDISCSSSEAISPINDALKKSLGLGESVAIGEHPSTPNSSTTCSSVEAAAGGSDS 122

Query: 122 AKSGEEVKEFEDKSGENCKKGNGRKIKKEKREKGPRFAFLTKTEIDHLEDGYRWRKYGQK 181
           AKSGE              K +GRK KKEKREKGPRFAFLTK+EID+LEDGYRWRKYGQK
Sbjct: 123 AKSGE-------------VKLDGRK-KKEKREKGPRFAFLTKSEIDNLEDGYRWRKYGQK 182

Query: 182 AVKNSPFPRSYYKCTTQNCSVKKRIERSTEDPCFVVTTYEGKHNHHCPITLRGQNAAGVL 241
           AVKNSPFPRSYYKCT+QNCSVKKR+ERS+EDPCFVVTTYEGKHNH+CPITLRG N AGV 
Sbjct: 183 AVKNSPFPRSYYKCTSQNCSVKKRVERSSEDPCFVVTTYEGKHNHYCPITLRGHNVAGVY 242

Query: 242 PASASPPLLPGLAPANFFSDEVYEGVDLQQQYYQVPDYGLLQDLINPLSFNRKQEP 298
           P SASP L+    PA   S E Y GVD+QQQYY+VP YG+L+DL+NP     KQ P
Sbjct: 243 PPSASPSLI----PARILSGEFYGGVDVQQQYYEVPGYGVLEDLVNP-----KQNP 249

BLAST of Lag0009743 vs. ExPASy Swiss-Prot
Match: Q8VWJ2 (WRKY transcription factor 28 OS=Arabidopsis thaliana OX=3702 GN=WRKY28 PE=2 SV=1)

HSP 1 Score: 155.6 bits (392), Expect = 8.7e-37
Identity = 106/249 (42.57%), Postives = 145/249 (58.23%), Query Frame = 0

Query: 1   MSNE-----DHQKDSCYQIFDPFTHNFHHHKPFEAPLLSPYDAFDPSYVGFFSDFLHASA 60
           MSNE     ++Q  S + + +    N     P     L   + F+PS    F+D L  S+
Sbjct: 1   MSNETRDLYNYQYPSSFSLHEMM--NLPTSNPSSYGNLPSQNGFNPSTYS-FTDCLQ-SS 60

Query: 61  DYDYNNILSTGLEMSCSSSEVISPIDDASKKSMGVGESVVTG---EHPSTPISSTASSSD 120
              Y ++L     +S SSSEV +   D  + +  V   V+ G       T +S + SSS 
Sbjct: 61  PAAYESLLQKTFGLSPSSSEVFNSSID-QEPNRDVTNDVINGGACNETETRVSPSNSSSS 120

Query: 121 EAAPGGGDSAKSGEEVKEFEDKSGENCKKGNGRKIKKEKREKGPRFAFLTKTEIDHLEDG 180
           EA   G DS KS  + +E   +  +  KK    K  + K+++ PR +F+TK+E+DHLEDG
Sbjct: 121 EADHPGEDSGKSRRK-RELVGEEDQISKKVGKTKKTEVKKQREPRVSFMTKSEVDHLEDG 180

Query: 181 YRWRKYGQKAVKNSPFPRSYYKCTTQNCSVKKRIERSTEDPCFVVTTYEGKHNHHCPITL 240
           YRWRKYGQKAVKNSP+PRSYY+CTTQ C+VKKR+ERS +DP  V+TTYEG+HNH  P  L
Sbjct: 181 YRWRKYGQKAVKNSPYPRSYYRCTTQKCNVKKRVERSFQDPTVVITTYEGQHNHPIPTNL 240

Query: 241 RGQNAAGVL 242
           RG +AA  +
Sbjct: 241 RGSSAAAAM 243

BLAST of Lag0009743 vs. ExPASy Swiss-Prot
Match: Q93WV4 (WRKY transcription factor 71 OS=Arabidopsis thaliana OX=3702 GN=WRKY71 PE=2 SV=1)

HSP 1 Score: 154.5 bits (389), Expect = 1.9e-36
Identity = 115/275 (41.82%), Postives = 143/275 (52.00%), Query Frame = 0

Query: 48  SDFLHASADYDYNNILST------GLEMSCSSSEVISPIDDASKKSMGVGESVVTGEHPS 107
           SD L +S   DYN++                S  V +P  + +  S  V  S   GE   
Sbjct: 22  SDCLQSSLVMDYNSLEKVFKFSPYSSPFQSVSPSVNNPYLNLTSNSPVVSSSSNEGE--- 81

Query: 108 TPISSTASSSDEAAPGGGDSAKSGEEVKEFEDKSGENCKKGNGRKIKKEKREKGPRFAFL 167
            P  +T   SD+     GD    GE  K+         K+G   K K EK+E+  R AF+
Sbjct: 82  -PKENTNDKSDQMEDNEGDLHGVGESSKQL-------TKQG---KKKGEKKEREVRVAFM 141

Query: 168 TKTEIDHLEDGYRWRKYGQKAVKNSPFPRSYYKCTTQNCSVKKRIERSTEDPCFVVTTYE 227
           TK+EIDHLEDGYRWRKYGQKAVKNSP+PRSYY+CTTQ C+VKKR+ERS +DP  V+TTYE
Sbjct: 142 TKSEIDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTTQKCNVKKRVERSFQDPSIVITTYE 201

Query: 228 GKHNHHCPITLRGQNAAGVL--------------PASASPPLLPGLAPANFFS--DEVYE 287
           GKHNH  P TLRG  AA  L              P      L+   +PAN+ S     YE
Sbjct: 202 GKHNHPIPSTLRGTVAAEHLLVHRGGGGSLLHSFPRHHQDFLMMKHSPANYQSVGSLSYE 261

Query: 288 GVDLQQQY-----YQVPDYGLLQDLINPLSFNRKQ 296
                  Y       V DYGLLQD++ P  F++ +
Sbjct: 262 HGHGTSSYNFNNNQPVVDYGLLQDIV-PSMFSKNE 281

BLAST of Lag0009743 vs. ExPASy Swiss-Prot
Match: Q9FL26 (WRKY transcription factor 8 OS=Arabidopsis thaliana OX=3702 GN=WRKY8 PE=1 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 2.6e-33
Identity = 111/314 (35.35%), Postives = 169/314 (53.82%), Query Frame = 0

Query: 20  HNFHHHKPFEAPLLSPYDAFDPSYVGFFS-DFLHASADYDYNNILSTGLEMSCSSSEVIS 79
           +N ++      P +S   A++ + +      F   S +         G E+S SSS+  +
Sbjct: 20  YNINNQNMINLPYVSGPSAYNANMISSSQVGFDLPSKNLSPQGAFELGFELSPSSSDFFN 79

Query: 80  PIDD------------ASKKS---MGVGESVVTGEHPSTPISSTASSSDEAAPGGGDSAK 139
           P  D            +S+KS   +G G + +  E     +S++ SSS+     G DS K
Sbjct: 80  PSLDQENGLYNAYNYNSSQKSHEVVGDGCATIKSE---VRVSASPSSSEADHHPGEDSGK 139

Query: 140 SGEEVKEFEDKSGENCKKGNG--RKIKKEKREKGPRFAFLTKTEIDHLEDGYRWRKYGQK 199
             ++ +E  D  GE+ ++     +  KKE+++K PR +F+TKTE+DHLEDGYRWRKYGQK
Sbjct: 140 IRKK-REVRD-GGEDDQRSQKVVKTKKKEEKKKEPRVSFMTKTEVDHLEDGYRWRKYGQK 199

Query: 200 AVKNSPFPRSYYKCTTQNCSVKKRIERSTEDPCFVVTTYEGKHNHHCPITLRGQNAAGVL 259
           AVKNSP+PRSYY+CTTQ C+VKKR+ERS +DP  V+TTYE +HNH  P   R    +G  
Sbjct: 200 AVKNSPYPRSYYRCTTQKCNVKKRVERSYQDPTVVITTYESQHNHPIPTNRRTAMFSGTT 259

Query: 260 PASASP---PLLPGL---APANFFSDEV----YEGVDLQQQYY--------QVPDYGLLQ 298
            +  +P   P+   L    P +F +D++    Y  V++   Y+        Q  ++ LL+
Sbjct: 260 ASDYNPSSSPIFSDLIINTPRSFSNDDLFRVPYASVNVNPSYHQQQHGFHQQESEFELLK 319

BLAST of Lag0009743 vs. ExPASy Swiss-Prot
Match: O22900 (WRKY transcription factor 23 OS=Arabidopsis thaliana OX=3702 GN=WRKY23 PE=2 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 1.1e-31
Identity = 77/148 (52.03%), Postives = 98/148 (66.22%), Query Frame = 0

Query: 100 PSTPISSTASS-SDEAAPGGGDSAKSGEEVKEFEDKSGENCKKGNGRKIKKEKREKGPRF 159
           P+TP SS+ SS S EA        +  EE    + +   + KK    K   +KR++  R 
Sbjct: 103 PATPNSSSISSASSEALNEEKPKTEDNEEEGGEDQQEKSHTKKQLKAKKNNQKRQREARV 162

Query: 160 AFLTKTEIDHLEDGYRWRKYGQKAVKNSPFPRSYYKCTTQNCSVKKRIERSTEDPCFVVT 219
           AF+TK+E+DHLEDGYRWRKYGQKAVKNSPFPRSYY+CTT +C+VKKR+ERS  DP  VVT
Sbjct: 163 AFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTTASCNVKKRVERSFRDPSTVVT 222

Query: 220 TYEGKHNHHCPITLRGQNAAGVLPASAS 247
           TYEG+H H  P+T R  +  G   +S +
Sbjct: 223 TYEGQHTHISPLTSRPISTGGFFGSSGA 250

BLAST of Lag0009743 vs. ExPASy Swiss-Prot
Match: Q9FGZ4 (Probable WRKY transcription factor 48 OS=Arabidopsis thaliana OX=3702 GN=WRKY48 PE=2 SV=1)

HSP 1 Score: 138.7 bits (348), Expect = 1.1e-31
Identity = 87/187 (46.52%), Postives = 117/187 (62.57%), Query Frame = 0

Query: 93  SVVTGEHPSTPISSTASSSDEAAPGGGDSAKSGEEVKEFEDKSGENCKKGN----GRKIK 152
           S V    P++P S++ SSS   A    +S K    VK+ E+   +  +KG       K K
Sbjct: 141 SEVVNTTPTSPNSTSVSSSSNEAANDNNSGKE-VTVKDQEEGDQQQEQKGTKPQLKAKKK 200

Query: 153 KEKREKGPRFAFLTKTEIDHLEDGYRWRKYGQKAVKNSPFPRSYYKCTTQNCSVKKRIER 212
            +K+ +  RFAFLTK++ID+L+DGYRWRKYGQKAVKNSP+PRSYY+CTT  C VKKR+ER
Sbjct: 201 NQKKAREARFAFLTKSDIDNLDDGYRWRKYGQKAVKNSPYPRSYYRCTTVGCGVKKRVER 260

Query: 213 STEDPCFVVTTYEGKHNHHCPITLRGQNAAGVLPASASPPLLPGLAPANFFSDEVYEGVD 272
           S++DP  V+TTYEG+H H  P+T RG    G+L    SP L  G   A+  S  + +   
Sbjct: 261 SSDDPSIVMTTYEGQHTHPFPMTPRGH--IGML---TSPILDHGATTASSSSFSIPQPRY 320

Query: 273 LQQQYYQ 276
           L  Q++Q
Sbjct: 321 LLTQHHQ 321

BLAST of Lag0009743 vs. ExPASy TrEMBL
Match: A0A0A0KBC5 (WRKY domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G139770 PE=4 SV=1)

HSP 1 Score: 365.9 bits (938), Expect = 1.6e-97
Identity = 211/311 (67.85%), Postives = 234/311 (75.24%), Query Frame = 0

Query: 1   MSNE--DHQKDS---CYQIFDPFTHNFHHHKPFEA--PLLSP-YDAFD--PSYVGFFSDF 60
           MSNE  DH+K+     Y  FDPFTHNFHH KPFE   PL +P Y+AFD  PS VGF+SDF
Sbjct: 1   MSNEGHDHEKEEDHPYYPQFDPFTHNFHHQKPFEVPPPLTNPYYEAFDIAPSSVGFYSDF 60

Query: 61  LHASADYDYNNILSTGLEMSCSSSEVISPIDDASKKSMGVG---ESVVTGEHPSTP-ISS 120
            HAS+ YDY+NI ST +EMSCSSSEVIS +DDA KKS  +G    SVVTGEHPSTP  SS
Sbjct: 61  SHASSQYDYHNIFSTPIEMSCSSSEVISSVDDALKKSSSLGRDLSSVVTGEHPSTPNCSS 120

Query: 121 TASSSDEAAPGGGDSAKSGEEVKEFEDKSGENCKKGNGRKIKKEKREKGPRFAFLTKTEI 180
           T  SSDE   GGGDS+KSG EVK F+DK GEN KK +  K KKEKREKGPRFAFLTKTEI
Sbjct: 121 TTCSSDEVVAGGGDSSKSG-EVKGFDDKKGENSKKVDEGK-KKEKREKGPRFAFLTKTEI 180

Query: 181 DHLEDGYRWRKYGQKAVKNSPFPRSYYKCTTQNCSVKKRIERSTEDPCFVVTTYEGKHNH 240
           D+LEDGYRWRKYGQKAVKNSPFPRSYYKCT+QNCSVKKR+ERS+EDP FV+TTYEGKHNH
Sbjct: 181 DNLEDGYRWRKYGQKAVKNSPFPRSYYKCTSQNCSVKKRVERSSEDPSFVITTYEGKHNH 240

Query: 241 HCPITLRGQNAAGVLPASASPPLLPGLAPANFFSDEVY-EGVDLQQQYYQVPDYGLLQDL 297
           +CPITLRG N  GVLP S +PPL P     NFFS E + E +  Q QY      GL QD 
Sbjct: 241 YCPITLRGHNPTGVLPPSVTPPLFP--PSPNFFSAESFCENLHQQYQY-----GGLFQDF 300

BLAST of Lag0009743 vs. ExPASy TrEMBL
Match: A0A1S3C4A4 (probable WRKY transcription factor 12 OS=Cucumis melo OX=3656 GN=LOC103496315 PE=4 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 6.0e-97
Identity = 211/310 (68.06%), Postives = 232/310 (74.84%), Query Frame = 0

Query: 1   MSNE--DHQK---DSCYQIFDPFTHNFHHHKPFEA--PLLSPY-DAFD--PSYVGFFSDF 60
           MSNE  DH+K   D  Y  FDPFTHNFHH KPFE   PL +PY +AFD  P  VGF+SDF
Sbjct: 1   MSNEGHDHEKEEDDPYYPQFDPFTHNFHHQKPFEVPPPLTNPYSEAFDIAPYNVGFYSDF 60

Query: 61  LHASADYDYNNILSTGLEMSCSSSEVISPIDDASKKSMGVG---ESVVTGEHPSTP-ISS 120
            HAS+ YDY+NI ST  EMSCSSSEVIS +DDA KKS  +G    SVVTGEHP TP  SS
Sbjct: 61  SHASSQYDYHNIFSTPFEMSCSSSEVISSVDDALKKSSSLGRDLSSVVTGEHPLTPNCSS 120

Query: 121 TASSSDEAAPGGGDSAKSGEEVKEFEDKSGENCKKGNGRKIKKEKREKGPRFAFLTKTEI 180
           T  SSDE   GGGDS+KSG EVK F+DK GEN KK +  K KKEKREKGPRFAFLTKTEI
Sbjct: 121 TTCSSDEVVAGGGDSSKSG-EVKGFDDKKGENSKKVDEGK-KKEKREKGPRFAFLTKTEI 180

Query: 181 DHLEDGYRWRKYGQKAVKNSPFPRSYYKCTTQNCSVKKRIERSTEDPCFVVTTYEGKHNH 240
           D+LEDGYRWRKYGQKAVKNSPFPRSYYKCT+QNCSVKKR+ERS+EDP FV+TTYEGKHNH
Sbjct: 181 DNLEDGYRWRKYGQKAVKNSPFPRSYYKCTSQNCSVKKRVERSSEDPGFVITTYEGKHNH 240

Query: 241 HCPITLRGQNAAGVLPASASPPLLPGLAPANFFSDEVYEGVDLQQQYYQVPDYGLLQDLI 297
           +CPITLRG N  GVLP S +PPL P     NFFS E +   +L QQY     YGL QD I
Sbjct: 241 YCPITLRGHNPTGVLPPSVTPPLFP--PSPNFFSAESF-CENLHQQY----QYGLFQDFI 300

BLAST of Lag0009743 vs. ExPASy TrEMBL
Match: A0A6J1FRU0 (probable WRKY transcription factor 28 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111448234 PE=4 SV=1)

HSP 1 Score: 346.3 bits (887), Expect = 1.3e-91
Identity = 193/296 (65.20%), Postives = 218/296 (73.65%), Query Frame = 0

Query: 2   SNEDHQKDSCYQIFDPFTHNFHHHKPFEAPLLSPYDAFDPSYVGFFSDFLHASADYDYNN 61
           +NEDH+K       DP+  NF              +AFDPS VGF+SDFL     YDYNN
Sbjct: 3   NNEDHEK-------DPYYPNF--------------EAFDPSNVGFYSDFL-----YDYNN 62

Query: 62  ILSTGLEMSCSSSEVISPIDDASKKSMGVGESVVTGEHPSTPISSTASSSDEAAPGGGDS 121
            LST +++SCSSSE ISPI+DA KKS+G+GESV TGEHPSTP SST  SS EAA GG DS
Sbjct: 63  FLSTDIDISCSSSEAISPINDALKKSLGLGESVATGEHPSTPNSSTTCSSVEAAAGGRDS 122

Query: 122 AKSGEEVKEFEDKSGENCKKGNGRKIKKEKREKGPRFAFLTKTEIDHLEDGYRWRKYGQK 181
           AKSGE              K +GRK KKEKREKGPRFAFLTK+EID+LEDGYRWRKYGQK
Sbjct: 123 AKSGE-------------VKVDGRK-KKEKREKGPRFAFLTKSEIDNLEDGYRWRKYGQK 182

Query: 182 AVKNSPFPRSYYKCTTQNCSVKKRIERSTEDPCFVVTTYEGKHNHHCPITLRGQNAAGVL 241
           AVKNSPFPRSYYKCT+QNCSVKKR+ERS+EDPCFVVTTYEGKHNH+CPITLRG N AGV 
Sbjct: 183 AVKNSPFPRSYYKCTSQNCSVKKRVERSSEDPCFVVTTYEGKHNHYCPITLRGHNVAGVY 242

Query: 242 PASASPPLLPGLAPANFFSDEVYEGVDLQQQYYQVPDYGLLQDLINPLSFNRKQEP 298
           P SASP L+    PA   + E Y GVD+QQQYY+VP YG+L+DL+NP     KQ P
Sbjct: 243 PPSASPSLI----PARILAGEFYGGVDVQQQYYEVPGYGVLEDLVNP-----KQNP 249

BLAST of Lag0009743 vs. ExPASy TrEMBL
Match: A0A6J1D7F3 (probable WRKY transcription factor 50 OS=Momordica charantia OX=3673 GN=LOC111018047 PE=4 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 1.3e-83
Identity = 197/306 (64.38%), Postives = 220/306 (71.90%), Query Frame = 0

Query: 1   MSNEDHQKDSCYQIFDPFTHNFHHHKPFEAPLLSPYDAFDPSYVGFFSDFLHASADYDYN 60
           MS ED +K       DP+ ++ H   P+ A        FDPS V  FSDFLH   DY++ 
Sbjct: 1   MSKEDEKKK------DPYYYDHHFDPPYGAD-------FDPSSVVGFSDFLH---DYNF- 60

Query: 61  NILSTGLEMSCSSSEVISPIDDASKKSMGVG-ESVVTGEHPSTPISSTASSSDEAA---P 120
           N  S  L+MSCSSSEVISP+DDASKKS GVG E  VTGEHP    SS+ SS D  A    
Sbjct: 61  NFFSAALDMSCSSSEVISPVDDASKKSSGVGAEWAVTGEHP----SSSCSSDDRVAAGGA 120

Query: 121 GGGDSAKSGEEV--KEFEDKSGENCKKGNGRKIKKEKREKGPRFAFLTKTEIDHLEDGYR 180
           GGGDSAKSG++V  KEFEDK GE CKK      KKEK EKGPR AFLTKTEID+LEDGYR
Sbjct: 121 GGGDSAKSGDQVVTKEFEDKDGEKCKKKVEGGKKKEKGEKGPRVAFLTKTEIDNLEDGYR 180

Query: 181 WRKYGQKAVKNSPFPRSYYKCTTQNCSVKKRIERSTEDPCFVVTTYEGKHNHHCPITLRG 240
           WRKYGQKAVKNSPFPRSYYKCTTQNC+VKKR+ERS++DP FVVTTYEGKHNH+CP+TLRG
Sbjct: 181 WRKYGQKAVKNSPFPRSYYKCTTQNCNVKKRVERSSQDPSFVVTTYEGKHNHYCPVTLRG 240

Query: 241 QNAAGVLPASASPPLLPGLAPANFFSDEVYEG-VDLQQQYYQV-PD-YGLLQDLINPLSF 298
             A   LP S++ P  P   PANFFS + Y+G VD  Q Y QV PD YGLLQDLI P SF
Sbjct: 241 NVAGMSLPLSSASPPWP---PANFFSGDPYKGVVDDHQDYSQVIPDHYGLLQDLI-PSSF 281

BLAST of Lag0009743 vs. ExPASy TrEMBL
Match: A0A6J1FT54 (probable WRKY transcription factor 48 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111448234 PE=4 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 4.1e-82
Identity = 183/296 (61.82%), Postives = 207/296 (69.93%), Query Frame = 0

Query: 2   SNEDHQKDSCYQIFDPFTHNFHHHKPFEAPLLSPYDAFDPSYVGFFSDFLHASADYDYNN 61
           +NEDH+K       DP+  NF              +AFDPS VGF+SDFL     YDYNN
Sbjct: 3   NNEDHEK-------DPYYPNF--------------EAFDPSNVGFYSDFL-----YDYNN 62

Query: 62  ILSTGLEMSCSSSEVISPIDDASKKSMGVGESVVTGEHPSTPISSTASSSDEAAPGGGDS 121
            LST +++SCSSSE ISPI+DA KKS+G+GESV TGEHPSTP SST  SS EAA GG DS
Sbjct: 63  FLSTDIDISCSSSEAISPINDALKKSLGLGESVATGEHPSTPNSSTTCSSVEAAAGGRDS 122

Query: 122 AKSGEEVKEFEDKSGENCKKGNGRKIKKEKREKGPRFAFLTKTEIDHLEDGYRWRKYGQK 181
           AKSGE              K +GRK KKEKREKGPRFAFLTK+EID+LEDGYRWRKYGQK
Sbjct: 123 AKSGE-------------VKVDGRK-KKEKREKGPRFAFLTKSEIDNLEDGYRWRKYGQK 182

Query: 182 AVKNSPFPRSYYKCTTQNCSVKKRIERSTEDPCFVVTTYEGKHNHHCPITLRGQNAAGVL 241
           AVKNSPFP           SVKKR+ERS+EDPCFVVTTYEGKHNH+CPITLRG N AGV 
Sbjct: 183 AVKNSPFP-----------SVKKRVERSSEDPCFVVTTYEGKHNHYCPITLRGHNVAGVY 238

Query: 242 PASASPPLLPGLAPANFFSDEVYEGVDLQQQYYQVPDYGLLQDLINPLSFNRKQEP 298
           P SASP L+    PA   + E Y GVD+QQQYY+VP YG+L+DL+NP     KQ P
Sbjct: 243 PPSASPSLI----PARILAGEFYGGVDVQQQYYEVPGYGVLEDLVNP-----KQNP 238

BLAST of Lag0009743 vs. TAIR 10
Match: AT4G18170.1 (WRKY DNA-binding protein 28 )

HSP 1 Score: 155.6 bits (392), Expect = 6.2e-38
Identity = 106/249 (42.57%), Postives = 145/249 (58.23%), Query Frame = 0

Query: 1   MSNE-----DHQKDSCYQIFDPFTHNFHHHKPFEAPLLSPYDAFDPSYVGFFSDFLHASA 60
           MSNE     ++Q  S + + +    N     P     L   + F+PS    F+D L  S+
Sbjct: 1   MSNETRDLYNYQYPSSFSLHEMM--NLPTSNPSSYGNLPSQNGFNPSTYS-FTDCLQ-SS 60

Query: 61  DYDYNNILSTGLEMSCSSSEVISPIDDASKKSMGVGESVVTG---EHPSTPISSTASSSD 120
              Y ++L     +S SSSEV +   D  + +  V   V+ G       T +S + SSS 
Sbjct: 61  PAAYESLLQKTFGLSPSSSEVFNSSID-QEPNRDVTNDVINGGACNETETRVSPSNSSSS 120

Query: 121 EAAPGGGDSAKSGEEVKEFEDKSGENCKKGNGRKIKKEKREKGPRFAFLTKTEIDHLEDG 180
           EA   G DS KS  + +E   +  +  KK    K  + K+++ PR +F+TK+E+DHLEDG
Sbjct: 121 EADHPGEDSGKSRRK-RELVGEEDQISKKVGKTKKTEVKKQREPRVSFMTKSEVDHLEDG 180

Query: 181 YRWRKYGQKAVKNSPFPRSYYKCTTQNCSVKKRIERSTEDPCFVVTTYEGKHNHHCPITL 240
           YRWRKYGQKAVKNSP+PRSYY+CTTQ C+VKKR+ERS +DP  V+TTYEG+HNH  P  L
Sbjct: 181 YRWRKYGQKAVKNSPYPRSYYRCTTQKCNVKKRVERSFQDPTVVITTYEGQHNHPIPTNL 240

Query: 241 RGQNAAGVL 242
           RG +AA  +
Sbjct: 241 RGSSAAAAM 243

BLAST of Lag0009743 vs. TAIR 10
Match: AT1G29860.1 (WRKY DNA-binding protein 71 )

HSP 1 Score: 154.5 bits (389), Expect = 1.4e-37
Identity = 115/275 (41.82%), Postives = 143/275 (52.00%), Query Frame = 0

Query: 48  SDFLHASADYDYNNILST------GLEMSCSSSEVISPIDDASKKSMGVGESVVTGEHPS 107
           SD L +S   DYN++                S  V +P  + +  S  V  S   GE   
Sbjct: 22  SDCLQSSLVMDYNSLEKVFKFSPYSSPFQSVSPSVNNPYLNLTSNSPVVSSSSNEGE--- 81

Query: 108 TPISSTASSSDEAAPGGGDSAKSGEEVKEFEDKSGENCKKGNGRKIKKEKREKGPRFAFL 167
            P  +T   SD+     GD    GE  K+         K+G   K K EK+E+  R AF+
Sbjct: 82  -PKENTNDKSDQMEDNEGDLHGVGESSKQL-------TKQG---KKKGEKKEREVRVAFM 141

Query: 168 TKTEIDHLEDGYRWRKYGQKAVKNSPFPRSYYKCTTQNCSVKKRIERSTEDPCFVVTTYE 227
           TK+EIDHLEDGYRWRKYGQKAVKNSP+PRSYY+CTTQ C+VKKR+ERS +DP  V+TTYE
Sbjct: 142 TKSEIDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTTQKCNVKKRVERSFQDPSIVITTYE 201

Query: 228 GKHNHHCPITLRGQNAAGVL--------------PASASPPLLPGLAPANFFS--DEVYE 287
           GKHNH  P TLRG  AA  L              P      L+   +PAN+ S     YE
Sbjct: 202 GKHNHPIPSTLRGTVAAEHLLVHRGGGGSLLHSFPRHHQDFLMMKHSPANYQSVGSLSYE 261

Query: 288 GVDLQQQY-----YQVPDYGLLQDLINPLSFNRKQ 296
                  Y       V DYGLLQD++ P  F++ +
Sbjct: 262 HGHGTSSYNFNNNQPVVDYGLLQDIV-PSMFSKNE 281

BLAST of Lag0009743 vs. TAIR 10
Match: AT5G46350.1 (WRKY DNA-binding protein 8 )

HSP 1 Score: 144.1 bits (362), Expect = 1.9e-34
Identity = 111/314 (35.35%), Postives = 169/314 (53.82%), Query Frame = 0

Query: 20  HNFHHHKPFEAPLLSPYDAFDPSYVGFFS-DFLHASADYDYNNILSTGLEMSCSSSEVIS 79
           +N ++      P +S   A++ + +      F   S +         G E+S SSS+  +
Sbjct: 20  YNINNQNMINLPYVSGPSAYNANMISSSQVGFDLPSKNLSPQGAFELGFELSPSSSDFFN 79

Query: 80  PIDD------------ASKKS---MGVGESVVTGEHPSTPISSTASSSDEAAPGGGDSAK 139
           P  D            +S+KS   +G G + +  E     +S++ SSS+     G DS K
Sbjct: 80  PSLDQENGLYNAYNYNSSQKSHEVVGDGCATIKSE---VRVSASPSSSEADHHPGEDSGK 139

Query: 140 SGEEVKEFEDKSGENCKKGNG--RKIKKEKREKGPRFAFLTKTEIDHLEDGYRWRKYGQK 199
             ++ +E  D  GE+ ++     +  KKE+++K PR +F+TKTE+DHLEDGYRWRKYGQK
Sbjct: 140 IRKK-REVRD-GGEDDQRSQKVVKTKKKEEKKKEPRVSFMTKTEVDHLEDGYRWRKYGQK 199

Query: 200 AVKNSPFPRSYYKCTTQNCSVKKRIERSTEDPCFVVTTYEGKHNHHCPITLRGQNAAGVL 259
           AVKNSP+PRSYY+CTTQ C+VKKR+ERS +DP  V+TTYE +HNH  P   R    +G  
Sbjct: 200 AVKNSPYPRSYYRCTTQKCNVKKRVERSYQDPTVVITTYESQHNHPIPTNRRTAMFSGTT 259

Query: 260 PASASP---PLLPGL---APANFFSDEV----YEGVDLQQQYY--------QVPDYGLLQ 298
            +  +P   P+   L    P +F +D++    Y  V++   Y+        Q  ++ LL+
Sbjct: 260 ASDYNPSSSPIFSDLIINTPRSFSNDDLFRVPYASVNVNPSYHQQQHGFHQQESEFELLK 319

BLAST of Lag0009743 vs. TAIR 10
Match: AT2G47260.1 (WRKY DNA-binding protein 23 )

HSP 1 Score: 138.7 bits (348), Expect = 7.8e-33
Identity = 77/148 (52.03%), Postives = 98/148 (66.22%), Query Frame = 0

Query: 100 PSTPISSTASS-SDEAAPGGGDSAKSGEEVKEFEDKSGENCKKGNGRKIKKEKREKGPRF 159
           P+TP SS+ SS S EA        +  EE    + +   + KK    K   +KR++  R 
Sbjct: 103 PATPNSSSISSASSEALNEEKPKTEDNEEEGGEDQQEKSHTKKQLKAKKNNQKRQREARV 162

Query: 160 AFLTKTEIDHLEDGYRWRKYGQKAVKNSPFPRSYYKCTTQNCSVKKRIERSTEDPCFVVT 219
           AF+TK+E+DHLEDGYRWRKYGQKAVKNSPFPRSYY+CTT +C+VKKR+ERS  DP  VVT
Sbjct: 163 AFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRCTTASCNVKKRVERSFRDPSTVVT 222

Query: 220 TYEGKHNHHCPITLRGQNAAGVLPASAS 247
           TYEG+H H  P+T R  +  G   +S +
Sbjct: 223 TYEGQHTHISPLTSRPISTGGFFGSSGA 250

BLAST of Lag0009743 vs. TAIR 10
Match: AT5G49520.1 (WRKY DNA-binding protein 48 )

HSP 1 Score: 138.7 bits (348), Expect = 7.8e-33
Identity = 87/187 (46.52%), Postives = 117/187 (62.57%), Query Frame = 0

Query: 93  SVVTGEHPSTPISSTASSSDEAAPGGGDSAKSGEEVKEFEDKSGENCKKGN----GRKIK 152
           S V    P++P S++ SSS   A    +S K    VK+ E+   +  +KG       K K
Sbjct: 141 SEVVNTTPTSPNSTSVSSSSNEAANDNNSGKE-VTVKDQEEGDQQQEQKGTKPQLKAKKK 200

Query: 153 KEKREKGPRFAFLTKTEIDHLEDGYRWRKYGQKAVKNSPFPRSYYKCTTQNCSVKKRIER 212
            +K+ +  RFAFLTK++ID+L+DGYRWRKYGQKAVKNSP+PRSYY+CTT  C VKKR+ER
Sbjct: 201 NQKKAREARFAFLTKSDIDNLDDGYRWRKYGQKAVKNSPYPRSYYRCTTVGCGVKKRVER 260

Query: 213 STEDPCFVVTTYEGKHNHHCPITLRGQNAAGVLPASASPPLLPGLAPANFFSDEVYEGVD 272
           S++DP  V+TTYEG+H H  P+T RG    G+L    SP L  G   A+  S  + +   
Sbjct: 261 SSDDPSIVMTTYEGQHTHPFPMTPRGH--IGML---TSPILDHGATTASSSSFSIPQPRY 320

Query: 273 LQQQYYQ 276
           L  Q++Q
Sbjct: 321 LLTQHHQ 321

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038902581.11.9e-11374.76probable WRKY transcription factor 56 [Benincasa hispida][more]
XP_011656990.13.2e-9767.85probable WRKY transcription factor 12 isoform X1 [Cucumis sativus] >KGN46828.1 h... [more]
XP_008456349.11.2e-9668.06PREDICTED: probable WRKY transcription factor 12 [Cucumis melo][more]
XP_022943476.12.7e-9165.20probable WRKY transcription factor 28 isoform X1 [Cucurbita moschata][more]
XP_023512278.14.5e-9165.20probable WRKY transcription factor 48 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q8VWJ28.7e-3742.57WRKY transcription factor 28 OS=Arabidopsis thaliana OX=3702 GN=WRKY28 PE=2 SV=1[more]
Q93WV41.9e-3641.82WRKY transcription factor 71 OS=Arabidopsis thaliana OX=3702 GN=WRKY71 PE=2 SV=1[more]
Q9FL262.6e-3335.35WRKY transcription factor 8 OS=Arabidopsis thaliana OX=3702 GN=WRKY8 PE=1 SV=1[more]
O229001.1e-3152.03WRKY transcription factor 23 OS=Arabidopsis thaliana OX=3702 GN=WRKY23 PE=2 SV=1[more]
Q9FGZ41.1e-3146.52Probable WRKY transcription factor 48 OS=Arabidopsis thaliana OX=3702 GN=WRKY48 ... [more]
Match NameE-valueIdentityDescription
A0A0A0KBC51.6e-9767.85WRKY domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G139770 PE=4 S... [more]
A0A1S3C4A46.0e-9768.06probable WRKY transcription factor 12 OS=Cucumis melo OX=3656 GN=LOC103496315 PE... [more]
A0A6J1FRU01.3e-9165.20probable WRKY transcription factor 28 isoform X1 OS=Cucurbita moschata OX=3662 G... [more]
A0A6J1D7F31.3e-8364.38probable WRKY transcription factor 50 OS=Momordica charantia OX=3673 GN=LOC11101... [more]
A0A6J1FT544.1e-8261.82probable WRKY transcription factor 48 isoform X2 OS=Cucurbita moschata OX=3662 G... [more]
Match NameE-valueIdentityDescription
AT4G18170.16.2e-3842.57WRKY DNA-binding protein 28 [more]
AT1G29860.11.4e-3741.82WRKY DNA-binding protein 71 [more]
AT5G46350.11.9e-3435.35WRKY DNA-binding protein 8 [more]
AT2G47260.17.8e-3352.03WRKY DNA-binding protein 23 [more]
AT5G49520.17.8e-3346.52WRKY DNA-binding protein 48 [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003657WRKY domainSMARTSM00774WRKY_clscoord: 169..228
e-value: 8.3E-37
score: 138.3
IPR003657WRKY domainPFAMPF03106WRKYcoord: 170..226
e-value: 8.8E-25
score: 86.7
IPR003657WRKY domainPROSITEPS50811WRKYcoord: 164..229
score: 31.605377
IPR036576WRKY domain superfamilyGENE3D2.20.25.80WRKY domaincoord: 155..230
e-value: 8.7E-33
score: 114.0
IPR036576WRKY domain superfamilySUPERFAMILY118290WRKY DNA-binding domaincoord: 162..229
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 82..151
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 123..144
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 98..114
NoneNo IPR availablePANTHERPTHR31221:SF211WRKY TRANSCRIPTION FACTOR 71-RELATEDcoord: 15..246
IPR044810WRKY transcription factor, plantPANTHERPTHR31221WRKY TRANSCRIPTION FACTOR PROTEIN 1-RELATEDcoord: 15..246

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0009743.1Lag0009743.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0043565 sequence-specific DNA binding