Lag0009693 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0009693 vs. NCBI nr
Match: KAG7034893.1 (Seed biotin-containing protein SBP65, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 617.8 bits (1592), Expect = 9.4e-173
Identity = 399/603 (66.17%), Postives = 429/603 (71.14%), Query Frame = 0

Query: 1   MATEQLSRRDNTTKERETHVEKERVPQLASHFEAIAVQGKGTDPSNAKATVPEKTEERQR 60
           MATEQLSRRD+TTKE+ET VE+ERVPQLASHFEAIAV GK TDPSN KAT PE+T+ RQR
Sbjct: 1   MATEQLSRRDDTTKEKETQVERERVPQLASHFEAIAVHGKTTDPSNEKATTPEETKARQR 60

Query: 61  SGASGGVGKEAHGTGIAKREVQGREEERQRRGGEKPEESHELATQFESLAGKVRDKRETD 120
           S AS GVGKE +GTGIA+  VQGREEERQ  GGEKPE+SHELA QFESLA KVRDKRET+
Sbjct: 61  SDAS-GVGKETYGTGIARGTVQGREEERQGVGGEKPEDSHELAAQFESLADKVRDKRETN 120

Query: 121 TENERERQARADRERTAAREREQGSRTREEQRASEQKVSEKEKGKEGEIGSRQNQPSLEE 180
           TENERERQARAD+ER AAREREQ  R  E++RASEQ      K KEGEIGSRQ+QP+LEE
Sbjct: 121 TENERERQARADKERIAAREREQKGRAEEKRRASEQ-----AKEKEGEIGSRQDQPTLEE 180

Query: 181 ISNYRAMAQEKSNEAIRAAKELYDRANKESLNQGIGARTEETGQRGTKGAERKVGEGGQQ 240
           ISNYRA+AQ+KSNEAI AA                                         
Sbjct: 181 ISNYRAIAQQKSNEAIMAA----------------------------------------- 240

Query: 241 TGLENVKERHDKANKESPNRGVGARTEETGQRGTKGAEHKVEEGGQQTGLENVKERYNKA 300
                                                                KERY+KA
Sbjct: 241 -----------------------------------------------------KERYDKA 300

Query: 301 NKESLNRGVGARTEERGHRGTEEAEGKVEEGGQQTGLETVTETLTSAAKTAKDYTTPIAE 360
           NK+SL +GV A T E   RGTEEAE  VEEG Q TGLETV ETLTSAAKTAKDYT PIAE
Sbjct: 301 NKKSLEKGVEAGTGEAIPRGTEEAEDTVEEGNQYTGLETVKETLTSAAKTAKDYTAPIAE 360

Query: 361 KAKDYTVQKVVEAKDMTVSAGQTTAHYLGEKAVAAKDVAIESGKVATEYAGKAAEDLKDK 420
           KAKDY           TVSAG+TTAHYLGEKAV AKDVAIESGKVA EYAGKAAEDLKDK
Sbjct: 361 KAKDY-----------TVSAGRTTAHYLGEKAVVAKDVAIESGKVAAEYAGKAAEDLKDK 420

Query: 421 AVVAGWSTAHYSCDTAMEGTKAAARLVKGAAEYAGAIAAKPLSAAKDVAESTGESLKDYT 480
             VAGWSTAHYSCDTA+EGTKAAARLVKGAAEYAGAIAAKPLSAAK+VAESTGE++KDYT
Sbjct: 421 TAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVAESTGETVKDYT 480

Query: 481 ARKKAEAQRESMYKQGEKETMYEKTKETFEGGKEEVKERGRGGRGDEKRVEEGRDDGGIL 540
           ARKK EAQRESMYKQ EKE+MYEKTKETFEGG EEVKERG G RG+E RVEE R DGGIL
Sbjct: 481 ARKKEEAQRESMYKQTEKESMYEKTKETFEGGIEEVKERG-GERGEENRVEEDRKDGGIL 491

Query: 541 GAIGETIYEIAQTTKEIVIGASD----------ELGQEEGEAKGQGYDKKEQYQTATRKE 594
           GAIGETIYEIAQTTKEIVIG  D          ELGQEEGE KGQ ++KKE Y+TA +KE
Sbjct: 541 GAIGETIYEIAQTTKEIVIGPGDETGKQSTLGFELGQEEGEEKGQEHEKKEHYETAAKKE 491

BLAST of Lag0009693 vs. NCBI nr
Match: KAG6604765.1 (Seed biotin-containing protein SBP65, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 615.9 bits (1587), Expect = 3.6e-172
Identity = 398/603 (66.00%), Postives = 428/603 (70.98%), Query Frame = 0

Query: 1   MATEQLSRRDNTTKERETHVEKERVPQLASHFEAIAVQGKGTDPSNAKATVPEKTEERQR 60
           MATEQLSRRD+TTKE+ET VE+ERVPQLASHFEAIAV GK TDPSN KAT PE+T+ RQR
Sbjct: 1   MATEQLSRRDDTTKEKETQVERERVPQLASHFEAIAVHGKTTDPSNEKATTPEETKARQR 60

Query: 61  SGASGGVGKEAHGTGIAKREVQGREEERQRRGGEKPEESHELATQFESLAGKVRDKRETD 120
           S AS GVGKE +GTGIA+  VQGREEERQ  GGEKPE+SHELA QFESLA KVRDKRET+
Sbjct: 61  SDAS-GVGKETYGTGIARGTVQGREEERQGVGGEKPEDSHELAAQFESLADKVRDKRETN 120

Query: 121 TENERERQARADRERTAAREREQGSRTREEQRASEQKVSEKEKGKEGEIGSRQNQPSLEE 180
           TENERERQARAD+ER AAREREQ  R  E++RASEQ      K KEGEIGSRQ+QP+LEE
Sbjct: 121 TENERERQARADKERIAAREREQKGREEEKRRASEQ-----AKEKEGEIGSRQDQPTLEE 180

Query: 181 ISNYRAMAQEKSNEAIRAAKELYDRANKESLNQGIGARTEETGQRGTKGAERKVGEGGQQ 240
           ISNYRA+AQ+KSNEAI AA                                         
Sbjct: 181 ISNYRAIAQQKSNEAIMAA----------------------------------------- 240

Query: 241 TGLENVKERHDKANKESPNRGVGARTEETGQRGTKGAEHKVEEGGQQTGLENVKERYNKA 300
                                                                KERY+KA
Sbjct: 241 -----------------------------------------------------KERYDKA 300

Query: 301 NKESLNRGVGARTEERGHRGTEEAEGKVEEGGQQTGLETVTETLTSAAKTAKDYTTPIAE 360
           NK+SL +GV A T E   RGTEEAE  VEEG Q TGLET  ETLTSAAKTAKDYT PIAE
Sbjct: 301 NKKSLEKGVEAGTGEAIPRGTEEAEDTVEEGNQYTGLETAKETLTSAAKTAKDYTAPIAE 360

Query: 361 KAKDYTVQKVVEAKDMTVSAGQTTAHYLGEKAVAAKDVAIESGKVATEYAGKAAEDLKDK 420
           KAKDY           TVSAG+TTAHYLGEKAV AKDVAIESGKVA EYAGKAAEDLKDK
Sbjct: 361 KAKDY-----------TVSAGRTTAHYLGEKAVVAKDVAIESGKVAAEYAGKAAEDLKDK 420

Query: 421 AVVAGWSTAHYSCDTAMEGTKAAARLVKGAAEYAGAIAAKPLSAAKDVAESTGESLKDYT 480
             VAGWSTAHYSCDTA+EGTKAAARLVKGAAEYAGAIAAKPLSAAK+VAESTGE++KDYT
Sbjct: 421 TAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVAESTGETVKDYT 480

Query: 481 ARKKAEAQRESMYKQGEKETMYEKTKETFEGGKEEVKERGRGGRGDEKRVEEGRDDGGIL 540
           ARKK EAQRESMYKQ EKE+MYEKTKETFEGG EEVKERG G RG+E RVEE R DGGIL
Sbjct: 481 ARKKEEAQRESMYKQTEKESMYEKTKETFEGGIEEVKERG-GERGEENRVEEDRKDGGIL 491

Query: 541 GAIGETIYEIAQTTKEIVIGASD----------ELGQEEGEAKGQGYDKKEQYQTATRKE 594
           GAIGETIYEIAQTTKEIVIG  D          ELGQEEGE KGQ ++KKE Y+TA +KE
Sbjct: 541 GAIGETIYEIAQTTKEIVIGPGDETGKQSTLGFELGQEEGEEKGQEHEKKEHYETAAKKE 491

BLAST of Lag0009693 vs. NCBI nr
Match: XP_022947224.1 (seed biotin-containing protein SBP65-like [Cucurbita moschata])

HSP 1 Score: 615.9 bits (1587), Expect = 3.6e-172
Identity = 399/603 (66.17%), Postives = 428/603 (70.98%), Query Frame = 0

Query: 1   MATEQLSRRDNTTKERETHVEKERVPQLASHFEAIAVQGKGTDPSNAKATVPEKTEERQR 60
           MATEQLSRRD+TTKE+ET VE+ERVPQLASHFEAIAV GK TDPSN KAT PE+T+ RQR
Sbjct: 1   MATEQLSRRDDTTKEKETQVERERVPQLASHFEAIAVHGKTTDPSNEKATTPEETKARQR 60

Query: 61  SGASGGVGKEAHGTGIAKREVQGREEERQRRGGEKPEESHELATQFESLAGKVRDKRETD 120
           S AS GVGKE +GTGIA+  VQGREEERQ  GGEKPE+SHELA QFESLA KVRDKRET+
Sbjct: 61  SDAS-GVGKETYGTGIARGTVQGREEERQGVGGEKPEDSHELAAQFESLADKVRDKRETN 120

Query: 121 TENERERQARADRERTAAREREQGSRTREEQRASEQKVSEKEKGKEGEIGSRQNQPSLEE 180
           TENERERQARAD+ER AAREREQ  R  E++RASEQ      K KEGEIGSRQ+QP+LEE
Sbjct: 121 TENERERQARADKERIAAREREQKGRAEEKRRASEQ-----AKEKEGEIGSRQDQPTLEE 180

Query: 181 ISNYRAMAQEKSNEAIRAAKELYDRANKESLNQGIGARTEETGQRGTKGAERKVGEGGQQ 240
           ISNYRA+AQ+KSNEAI AA                                         
Sbjct: 181 ISNYRAIAQQKSNEAIMAA----------------------------------------- 240

Query: 241 TGLENVKERHDKANKESPNRGVGARTEETGQRGTKGAEHKVEEGGQQTGLENVKERYNKA 300
                                                                KERY+KA
Sbjct: 241 -----------------------------------------------------KERYDKA 300

Query: 301 NKESLNRGVGARTEERGHRGTEEAEGKVEEGGQQTGLETVTETLTSAAKTAKDYTTPIAE 360
           NK+SL +GV A T E   RGTEEAE  VEEG Q TGLETV ETLTSAAKTAKDYT PIAE
Sbjct: 301 NKKSLEKGVEAGTGEAIPRGTEEAEDTVEEGNQYTGLETVKETLTSAAKTAKDYTAPIAE 360

Query: 361 KAKDYTVQKVVEAKDMTVSAGQTTAHYLGEKAVAAKDVAIESGKVATEYAGKAAEDLKDK 420
           KAKDY           TVSAG+TTAHYLGEKAV AKDVAIESGKVA EYAGKAAEDLKDK
Sbjct: 361 KAKDY-----------TVSAGRTTAHYLGEKAVVAKDVAIESGKVAAEYAGKAAEDLKDK 420

Query: 421 AVVAGWSTAHYSCDTAMEGTKAAARLVKGAAEYAGAIAAKPLSAAKDVAESTGESLKDYT 480
             VAGWSTAHYSCDTA+EGTKAAARLVKGAAEYAGAIAAKPLSAAK+VAESTGE++KDYT
Sbjct: 421 TAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVAESTGETVKDYT 480

Query: 481 ARKKAEAQRESMYKQGEKETMYEKTKETFEGGKEEVKERGRGGRGDEKRVEEGRDDGGIL 540
           ARKK EAQRESMYKQ EKE+MYEKTKETFEGG EEVKERG G RG+E RVEE R DGGIL
Sbjct: 481 ARKKEEAQRESMYKQTEKESMYEKTKETFEGGIEEVKERG-GERGEENRVEEDRKDGGIL 491

Query: 541 GAIGETIYEIAQTTKEIVIGASD----------ELGQEEGEAKGQGYDKKEQYQTATRKE 594
           GAIGETIYEIAQTTKEIVIG  D          ELGQEEGE KGQ + KKE Y+TA +KE
Sbjct: 541 GAIGETIYEIAQTTKEIVIGPGDETGKQSTLGFELGQEEGEEKGQEHVKKEHYETAAKKE 491

BLAST of Lag0009693 vs. NCBI nr
Match: XP_023534218.1 (seed biotin-containing protein SBP65-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 615.5 bits (1586), Expect = 4.7e-172
Identity = 400/604 (66.23%), Postives = 429/604 (71.03%), Query Frame = 0

Query: 1   MATEQLSRRDNTTKERETHVEKERVPQLASHFEAIAVQGKGTDPSNAKATVPEKTEERQR 60
           MATE+LSRRD+TTKE+ET VE+ERVPQLASHFEAIAV GK TDPSN KAT PE+T+ RQR
Sbjct: 1   MATEKLSRRDDTTKEKETQVERERVPQLASHFEAIAVHGKATDPSNEKATTPEETKARQR 60

Query: 61  SGASGGVGKEAHGTGIAKREVQGREEERQRRGGEKPEESHELATQFESLAGKVRDKRETD 120
           S AS GVGKE +GTGIA+  VQGREEERQ  GGEKPE+SHELA QFESLA KVRDKRET+
Sbjct: 61  SDAS-GVGKETYGTGIARGTVQGREEERQGVGGEKPEDSHELAAQFESLADKVRDKRETN 120

Query: 121 TENERERQARADRERTAAREREQ-GSRTREEQRASEQKVSEKEKGKEGEIGSRQNQPSLE 180
           TENERERQARAD+ER AAREREQ GSR  E+QR     VSE+ K KEGEIGSRQNQPSLE
Sbjct: 121 TENERERQARADKERIAAREREQKGSRAEEKQR-----VSEQAKEKEGEIGSRQNQPSLE 180

Query: 181 EISNYRAMAQEKSNEAIRAAKELYDRANKESLNQGIGARTEETGQRGTKGAERKVGEGGQ 240
           EISNYRA+AQ+KSNEAI AA                                        
Sbjct: 181 EISNYRAIAQQKSNEAIMAA---------------------------------------- 240

Query: 241 QTGLENVKERHDKANKESPNRGVGARTEETGQRGTKGAEHKVEEGGQQTGLENVKERYNK 300
                                                                 KERY+K
Sbjct: 241 ------------------------------------------------------KERYDK 300

Query: 301 ANKESLNRGVGARTEERGHRGTEEAEGKVEEGGQQTGLETVTETLTSAAKTAKDYTTPIA 360
           ANK+SL +GV A T E   RGTEEAE  VEEG Q TGLETV ETLTSAAKTAKDYT PIA
Sbjct: 301 ANKKSLQQGVEAGTGEAIPRGTEEAEDTVEEGNQYTGLETVKETLTSAAKTAKDYTAPIA 360

Query: 361 EKAKDYTVQKVVEAKDMTVSAGQTTAHYLGEKAVAAKDVAIESGKVATEYAGKAAEDLKD 420
           EKAKDY           TVSAG+TTAHYLGEKAV AKDVAIESGKVA  YAGKAAEDLKD
Sbjct: 361 EKAKDY-----------TVSAGRTTAHYLGEKAVVAKDVAIESGKVAAGYAGKAAEDLKD 420

Query: 421 KAVVAGWSTAHYSCDTAMEGTKAAARLVKGAAEYAGAIAAKPLSAAKDVAESTGESLKDY 480
           K  VAGWSTAHYSCDTA+EGTKAAARLVKGAAEYAGAIAAKPLSAAK+VAESTGE++KDY
Sbjct: 421 KTAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVAESTGETVKDY 480

Query: 481 TARKKAEAQRESMYKQGEKETMYEKTKETFEGGKEEVKERGRGGRGDEKRVEEGRDDGGI 540
           TARKK EAQRESMYKQ EK++MYEKTKETFEGG EEVKERG G RG E RVEE R DGGI
Sbjct: 481 TARKKEEAQRESMYKQTEKDSMYEKTKETFEGGIEEVKERG-GERGGENRVEEDRSDGGI 492

Query: 541 LGAIGETIYEIAQTTKEIVIGASD----------ELGQEEGEAKGQGYDKKEQYQTATRK 594
           LGAIGETIYEIAQTTKEIVIG  D          ELGQEEGE KGQ ++KKE Y+TA +K
Sbjct: 541 LGAIGETIYEIAQTTKEIVIGPGDETGKQSTLGFELGQEEGEEKGQEHEKKEHYETAAKK 492

BLAST of Lag0009693 vs. NCBI nr
Match: XP_022970889.1 (seed biotin-containing protein SBP65-like [Cucurbita maxima])

HSP 1 Score: 607.8 bits (1566), Expect = 9.8e-170
Identity = 395/604 (65.40%), Postives = 427/604 (70.70%), Query Frame = 0

Query: 1   MATEQLSRRDNTTKERETHVEKERVPQLASHFEAIAVQGKGTDPSNAKATVPEKTEERQR 60
           MATE+LSRRD+TTKE+E  VE+ERVPQL SHFEAIAV GK TDPSN KAT PE+T+ RQ+
Sbjct: 1   MATEKLSRRDDTTKEKEIQVERERVPQLTSHFEAIAVHGKATDPSNEKATTPEETKARQK 60

Query: 61  SGASGGVGKEAHGTGIAKREVQGREEERQRRGGEKPEESHELATQFESLAGKVRDKRETD 120
           S AS GVGKE +GTGIA+  VQGREEERQ  GGEKPE+SHELA QFESLA KVRDKRETD
Sbjct: 61  SDAS-GVGKETYGTGIARGTVQGREEERQGVGGEKPEDSHELAAQFESLADKVRDKRETD 120

Query: 121 TENERERQARADRERTAAREREQ-GSRTREEQRASEQKVSEKEKGKEGEIGSRQNQPSLE 180
           TENERERQARAD+ER AAREREQ GSR  E+QRA EQ      K +EGEIGSRQNQPSLE
Sbjct: 121 TENERERQARADKERIAAREREQKGSRAEEKQRALEQ-----AKEREGEIGSRQNQPSLE 180

Query: 181 EISNYRAMAQEKSNEAIRAAKELYDRANKESLNQGIGARTEETGQRGTKGAERKVGEGGQ 240
           EISNYRA+AQ+KSNEAI AA                                        
Sbjct: 181 EISNYRAIAQQKSNEAIMAA---------------------------------------- 240

Query: 241 QTGLENVKERHDKANKESPNRGVGARTEETGQRGTKGAEHKVEEGGQQTGLENVKERYNK 300
                                                                 KERY+K
Sbjct: 241 ------------------------------------------------------KERYDK 300

Query: 301 ANKESLNRGVGARTEERGHRGTEEAEGKVEEGGQQTGLETVTETLTSAAKTAKDYTTPIA 360
           ANK+SL +GV A T E   RGTEEAE  VEEG Q +GLETV ETLTSAAKTAKDYT PIA
Sbjct: 301 ANKKSLEQGVEAGTREAIPRGTEEAEDTVEEGNQYSGLETVKETLTSAAKTAKDYTAPIA 360

Query: 361 EKAKDYTVQKVVEAKDMTVSAGQTTAHYLGEKAVAAKDVAIESGKVATEYAGKAAEDLKD 420
           EKAKDY           TVSAG+TTAHYLGEKAV AKDVAIESGKVA EYAGKAAEDLKD
Sbjct: 361 EKAKDY-----------TVSAGRTTAHYLGEKAVVAKDVAIESGKVAAEYAGKAAEDLKD 420

Query: 421 KAVVAGWSTAHYSCDTAMEGTKAAARLVKGAAEYAGAIAAKPLSAAKDVAESTGESLKDY 480
           K  VAGWSTAHYSCDTA+EGTKAAARLVKGAAEYAGAIAAKPLSAAK+VAESTGE++KDY
Sbjct: 421 KTAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVAESTGETVKDY 480

Query: 481 TARKKAEAQRESMYKQGEKETMYEKTKETFEGGKEEVKERGRGGRGDEKRVEEGRDDGGI 540
           TARKK EAQRESMYKQ EKE+MYEKTKETFE G EEVKERG G RG+E RVEE R+DGGI
Sbjct: 481 TARKKEEAQRESMYKQTEKESMYEKTKETFEEGIEEVKERG-GERGEENRVEEDRNDGGI 492

Query: 541 LGAIGETIYEIAQTTKEIVIGASD----------ELGQEEGEAKGQGYDKKEQYQTATRK 594
           LGAIGETIYEIAQTTK+IVIG  D          ELGQEE E KGQ ++KKE Y+TA +K
Sbjct: 541 LGAIGETIYEIAQTTKDIVIGPGDETGKQSTLGFELGQEEDEEKGQEHEKKEHYETAAKK 492

BLAST of Lag0009693 vs. ExPASy Swiss-Prot
Match: Q39846 (Seed biotin-containing protein SBP65 OS=Glycine max OX=3847 GN=SBP65 PE=2 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 6.6e-44
Identity = 205/590 (34.75%), Postives = 287/590 (48.64%), Query Frame = 0

Query: 1   MATEQLSRRDNTTKERETHVEKERVPQLASHFEAIAVQGKGTDPSNAKATVPEKTEERQR 60
           MA+EQL+RR+NTT E+E HVEK RVP++A+HFE +A Q K +D +  K T P+ + E  +
Sbjct: 1   MASEQLARRENTTTEKEIHVEKHRVPKMATHFEHLAEQAKESDITAGKDT-PQGSIEALQ 60

Query: 61  SGASGGVGKEAHGTGIAKREVQGREEERQRRGGEKPEESHELATQFESLAGKVRDKRETD 120
           +G      +     G A  ++ GR + R         E+HEL   FESLA KV D    +
Sbjct: 61  AGE-----RVKDHAGKAMGDIGGRGKAR---------ETHELGAHFESLADKVTDHAAAN 120

Query: 121 TENERERQARADRERTAAREREQGSRTREEQRASEQKVSEKEKGKEGEIGSRQNQPSLEE 180
               +E Q                     E R   + V + E   EG     +++P L+ 
Sbjct: 121 VVGNKESQ--------------------REARGGVRDVGKFEMRTEGGEKGNKDRPELK- 180

Query: 181 ISNYRAMAQEKSNEAIRAAKELYDRANKESLNQGIGARTEETGQRGTKGAERKVGEGGQQ 240
                              +E+  R  KE   +  G    E      KG E +   G  +
Sbjct: 181 ----------------TRTREVIGRTEKERGRESGGQVVAE------KGRETETARG--R 240

Query: 241 TGLENVKERHDKANKESPNRGVGA----RTEETGQRGTKGAEHKVEEGGQQTGLENVKER 300
            G EN   R       +  +G G     R EE      + A  ++     Q   + VKE+
Sbjct: 241 VGAENEGARTTAVITCTLEKGGGTQKPIREEERESESERSAWEQISNYSDQ-ATQGVKEK 300

Query: 301 YNKANK---ESLNRGVGARTEERGHRGTEEAEGK--VEEGGQQTGLETVTETLTSAAKTA 360
           Y +A +   E+LN       E+        A+ K    E GQQ G     +T++SAAKTA
Sbjct: 301 YERAKQAASETLNTTTQTAQEKSAQAKNLAAQAKDATLEKGQQ-GYAVTKDTISSAAKTA 360

Query: 361 KDYTTPIAEKAKDYTVQKVVEAKDMTVSAGQTTAHYLGEKAVAAKDVAIESGKVATEYAG 420
            + T P+AEKAKDYT+Q   +AK    SAG TTA Y+GEKAV AKDVA+ESGK A  YA 
Sbjct: 361 SEKTAPVAEKAKDYTLQAAEKAK----SAGGTTASYVGEKAVQAKDVAVESGKSAAGYAA 420

Query: 421 KAAEDLKDKAVVAGWSTAHYSCDTAMEGTKAAARLVKGAAEYAGAIAAKPLS----AAKD 480
           K A DL+DKA   GW+ AH+S +  +EGTKAAA +V+GAA YAG  AA+  S    A K 
Sbjct: 421 KVAADLRDKATAVGWAAAHFSAEKTVEGTKAAAHVVEGAAGYAGHKAAELASMSAGAVKG 480

Query: 481 VAESTGESLKDYTARKKAEAQRESMYKQGEKETMYEKTKETFEGGKEEVKERGRGGRGDE 540
           +A S GE+ K+YTA+KK EAQRE   K+  +    E  +   EG  E V++  +  +  E
Sbjct: 481 LAASAGETAKEYTAKKKEEAQRELEAKKPSQP--QEAEERPSEGIGETVRQYAQKPKPSE 522

Query: 541 KRVEEGRDDGGILGAIGETIYEIAQTTK---EIVIGASDE--LGQEEGEA 573
              ++      +  AIGET+    +  K   +  +G   E   G+EEG++
Sbjct: 541 GNPQKEGTGSIVFTAIGETVSSAGEKVKKPFKNTMGGESEGGGGKEEGKS 522

BLAST of Lag0009693 vs. ExPASy Swiss-Prot
Match: Q41060 (Seed biotin-containing protein SBP65 OS=Pisum sativum OX=3888 GN=SBP65 PE=1 SV=1)

HSP 1 Score: 113.2 bits (282), Expect = 9.9e-24
Identity = 184/629 (29.25%), Postives = 269/629 (42.77%), Query Frame = 0

Query: 1   MATEQLSRRDNTTKERETHVEKERVPQLASHFEAIAVQGKGTDPSNAKATVPEKTEERQR 60
           MA+EQLSRR+N T ER+    ++ VPQ  +HFE       G    N ++    + +    
Sbjct: 1   MASEQLSRRENITTERKIQNAEDSVPQRTTHFELRETHELG---PNFQSLPRNENQAYLD 60

Query: 61  SGASGGVGKEAHGTGIAKREVQGREEERQRRGGEKPEESHELATQFESLAGKVRDKRETD 120
            GA   +      + + +  V       + R  EK +             G VRD  +  
Sbjct: 61  RGARAPLSANVSESYLDRARVPLNANIPEHRVREKED------------FGGVRDMGKFQ 120

Query: 121 TENERERQARA-DRERTAARER-EQGSRTREEQRASEQKVSEKEKGKEGEIGSRQNQPSL 180
            E++   ++ A DRE    R R   G+   +E     Q V E+E+ +E  +   + + ++
Sbjct: 121 MESKGGNKSLAEDRETLDTRSRMVTGTPHIKEASGKGQVVEERERARERAMEEEEKRLTM 180

Query: 181 EEISNYRAMAQEKSNEAIRAAKELYDRANKESLNQGIGARTEETGQRGTKGAERKVGEGG 240
           EEIS YR  AQ+ + EA+ AA+E Y+RA +          T ET +  T+ A+ K GE  
Sbjct: 181 EEISKYRNQAQQSALEALSAAQEKYERAKQ---------ATNETLRNTTQAAQEK-GEAA 240

Query: 241 QQTGLENVKERHDKANKESPNRGVGARTEETGQRGTKGAEHKVEEGGQQTGLENVKERYN 300
           Q             A  E   +G     E TG   +  A    E+  Q            
Sbjct: 241 QA----------KDATFEKTQQGY----EMTGDTVSNSARTASEKAAQ------------ 300

Query: 301 KANKESLNRGVGARTEERGHRGTEEAEGKVEEGGQQTGLETVTETLTSAAKTAKDYTTPI 360
            A   +L                         G  Q G E   +T+++AA+TA +Y TP 
Sbjct: 301 -AKNTTL-------------------------GKTQQGYEATRDTVSNAARTAAEYATPA 360

Query: 361 AEKAKDYTVQKVVEAKDMTVSAGQTTAHYLGEKAVAAKDVAIESGKVATEYAGKAAEDLK 420
           AEKA+   VQ    AKD+T+  G+T A               E  K A E A K A DLK
Sbjct: 361 AEKARCVAVQ----AKDVTLETGKTAA---------------EKAKCAAEIAAKVAVDLK 420

Query: 421 DKAVVAGWSTAHYSCDTAMEGTKAAARLVKGAAEY----AGAIAAKPLSAAKDVAESTGE 480
           +KA VAGW+ +HY+    ++GT+AAA  V+GA  Y    A  +AAK +   K +A S GE
Sbjct: 421 EKATVAGWTASHYATQLTVDGTRAAANAVEGAVGYVAPKASELAAKSVETVKGLAASAGE 480

Query: 481 SLKDYTARKKA------EAQRESMYKQGEK---------ETMYEKTKETFEGGKEEVKER 540
           + K++TARKK       EA+R S  ++GE+         + +    +   +G   + K +
Sbjct: 481 TAKEFTARKKEESWREYEAKRASQLQEGEEILPSTGGIGKVLPSGERTQAQGTNLQEKVQ 531

Query: 541 GRGG------------------------------RGDEKRVEEGRDDGGILGAIGETIYE 579
           G+G                                G      +G+D GG+L AIGETI E
Sbjct: 541 GKGSDILGAVTETVSDIGSSMIKPIDNANTKVKEHGGTTITPKGQDAGGVLDAIGETIAE 531

BLAST of Lag0009693 vs. ExPASy Swiss-Prot
Match: Q9SKP0 (Late embryogenesis abundant protein ECP63 OS=Arabidopsis thaliana OX=3702 GN=ECP63 PE=2 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 9.0e-09
Identity = 87/287 (30.31%), Postives = 118/287 (41.11%), Query Frame = 0

Query: 321 TEEAEGKVEEGGQQTGLETV--TETLTSAAKTAKDYTTPIAEKAKDYTVQKVVEAKDMTV 380
           T EA    +EG Q    + V   +     AK   DYT     + KDYTV K  EAKD T 
Sbjct: 77  THEAAESTKEGAQIASEKAVGAKDATVEKAKETADYTAEKVGEYKDYTVDKAKEAKDTTA 136

Query: 381 SAGQTTAHYLGEKAVAAKDVAIESGKVATEYAGKAAEDLKDKAVVAGWSTAHYSCDTAME 440
              + TA+Y  +KAV AKD   E      +YA   A + KDK       TA+Y+ D A E
Sbjct: 137 EKAKETANYTADKAVEAKDKTAEKIGEYKDYAVDKAVEAKDKTAEKAKETANYTADKAKE 196

Query: 441 GTKAAAR--------LVKGAAEYAGAIAAKPLSAAKDVAESTGESLKDYTARKKAEAQRE 500
                A          V  A E     A K + A    AE TGE  KDYT  K  E +  
Sbjct: 197 AKDKTAEKVGEYKDYTVDKAVEARDYTAEKAIEAKDKTAEKTGE-YKDYTVEKATEGKDV 256

Query: 501 SMYKQGE-KETMYEKTKET---FEGGKEEVKERG------------RGGRGDEKRVEEGR 560
           ++ K GE K++  E  K       G  EE K +             + G    +++EE R
Sbjct: 257 TVSKLGELKDSAVETAKRAMGFLSGKTEEAKGKAVETKDTAKENMEKAGEVTRQKMEEMR 316

Query: 561 DDGGIL-GAIGETIYEIAQTTKEIVIGASDELGQEEGEAKGQGYDKK 581
            +G  L    G    E +Q T+E     + +  + +  A  +G + K
Sbjct: 317 LEGKELKEEAGAKAQEASQKTRESTESGAQKAEETKDSAAVRGNEAK 362

BLAST of Lag0009693 vs. ExPASy Swiss-Prot
Match: Q9LF88 (Late embryogenesis abundant protein At3g53040 OS=Arabidopsis thaliana OX=3702 GN=At3g53040 PE=2 SV=1)

HSP 1 Score: 52.4 bits (124), Expect = 2.1e-05
Identity = 89/309 (28.80%), Postives = 120/309 (38.83%), Query Frame = 0

Query: 303 ESLNRGVGARTEERGHRGTEEAEGKVEEGGQQTGLETVTETLTSAAKTAKDYTTPIAEKA 362
           ES   G    +E+    G  +  G+V +   Q   ET   T    A+ AKD T    ++ 
Sbjct: 78  ESTREGADIASEKAA--GMRDTTGEVRDSTAQKTKETADYT-ADKAREAKDKTADKTKET 137

Query: 363 KDYTVQKVVEAKDMTVSAGQTTAHYLGEKAVAAKDVAIESGKVATEYAGKAAEDLKDKAV 422
            DY  +K  EAKD T    + TA Y  EKA  AKD   +      +Y  + A++ KD   
Sbjct: 138 ADYAAEKAREAKDRTADKTKETAEYTAEKAREAKDKTADKLGEYKDYTAEKAKEAKDTTA 197

Query: 423 VAGWSTAHYSCDTAMEGTKAAARLVKGAAEYAGAIAAKPLSAAKDVAESTGESLKDYTAR 482
                   Y+ D A E     A   K  AEY    + K        AE  GE  KDYTA 
Sbjct: 198 EKLGEYKDYTVDKAKEAKDKTAEKAKETAEYT---SDKARETKDKTAEKVGE-YKDYTAE 257

Query: 483 K---KAEAQRESMYKQGEKETMY-----EKTKETFEGGKEEVKERGRGGRGDEKRVEEGR 542
           K    A+  RE+  K  EK   Y     EK  ET + G  ++      G   +  V+  +
Sbjct: 258 KAKETADKAREAKDKTAEKVGEYRDYTAEKATETKDAGVSKI------GELKDSAVDTAK 317

Query: 543 DDGGILGAIGETIYEIAQTTKEIVIGASDELGQE----------EGEAKGQGYDKKEQYQ 592
              G L    E   + A  TK+      DE G+E          EG+   +   +K Q  
Sbjct: 318 RAMGFLSGKTEETKQKAVETKDTAKEKMDEAGEEARRKMEEMRLEGKKLDEDASRKTQQS 373

BLAST of Lag0009693 vs. ExPASy TrEMBL
Match: A0A6J1G664 (seed biotin-containing protein SBP65-like OS=Cucurbita moschata OX=3662 GN=LOC111451152 PE=4 SV=1)

HSP 1 Score: 615.9 bits (1587), Expect = 1.7e-172
Identity = 399/603 (66.17%), Postives = 428/603 (70.98%), Query Frame = 0

Query: 1   MATEQLSRRDNTTKERETHVEKERVPQLASHFEAIAVQGKGTDPSNAKATVPEKTEERQR 60
           MATEQLSRRD+TTKE+ET VE+ERVPQLASHFEAIAV GK TDPSN KAT PE+T+ RQR
Sbjct: 1   MATEQLSRRDDTTKEKETQVERERVPQLASHFEAIAVHGKTTDPSNEKATTPEETKARQR 60

Query: 61  SGASGGVGKEAHGTGIAKREVQGREEERQRRGGEKPEESHELATQFESLAGKVRDKRETD 120
           S AS GVGKE +GTGIA+  VQGREEERQ  GGEKPE+SHELA QFESLA KVRDKRET+
Sbjct: 61  SDAS-GVGKETYGTGIARGTVQGREEERQGVGGEKPEDSHELAAQFESLADKVRDKRETN 120

Query: 121 TENERERQARADRERTAAREREQGSRTREEQRASEQKVSEKEKGKEGEIGSRQNQPSLEE 180
           TENERERQARAD+ER AAREREQ  R  E++RASEQ      K KEGEIGSRQ+QP+LEE
Sbjct: 121 TENERERQARADKERIAAREREQKGRAEEKRRASEQ-----AKEKEGEIGSRQDQPTLEE 180

Query: 181 ISNYRAMAQEKSNEAIRAAKELYDRANKESLNQGIGARTEETGQRGTKGAERKVGEGGQQ 240
           ISNYRA+AQ+KSNEAI AA                                         
Sbjct: 181 ISNYRAIAQQKSNEAIMAA----------------------------------------- 240

Query: 241 TGLENVKERHDKANKESPNRGVGARTEETGQRGTKGAEHKVEEGGQQTGLENVKERYNKA 300
                                                                KERY+KA
Sbjct: 241 -----------------------------------------------------KERYDKA 300

Query: 301 NKESLNRGVGARTEERGHRGTEEAEGKVEEGGQQTGLETVTETLTSAAKTAKDYTTPIAE 360
           NK+SL +GV A T E   RGTEEAE  VEEG Q TGLETV ETLTSAAKTAKDYT PIAE
Sbjct: 301 NKKSLEKGVEAGTGEAIPRGTEEAEDTVEEGNQYTGLETVKETLTSAAKTAKDYTAPIAE 360

Query: 361 KAKDYTVQKVVEAKDMTVSAGQTTAHYLGEKAVAAKDVAIESGKVATEYAGKAAEDLKDK 420
           KAKDY           TVSAG+TTAHYLGEKAV AKDVAIESGKVA EYAGKAAEDLKDK
Sbjct: 361 KAKDY-----------TVSAGRTTAHYLGEKAVVAKDVAIESGKVAAEYAGKAAEDLKDK 420

Query: 421 AVVAGWSTAHYSCDTAMEGTKAAARLVKGAAEYAGAIAAKPLSAAKDVAESTGESLKDYT 480
             VAGWSTAHYSCDTA+EGTKAAARLVKGAAEYAGAIAAKPLSAAK+VAESTGE++KDYT
Sbjct: 421 TAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVAESTGETVKDYT 480

Query: 481 ARKKAEAQRESMYKQGEKETMYEKTKETFEGGKEEVKERGRGGRGDEKRVEEGRDDGGIL 540
           ARKK EAQRESMYKQ EKE+MYEKTKETFEGG EEVKERG G RG+E RVEE R DGGIL
Sbjct: 481 ARKKEEAQRESMYKQTEKESMYEKTKETFEGGIEEVKERG-GERGEENRVEEDRKDGGIL 491

Query: 541 GAIGETIYEIAQTTKEIVIGASD----------ELGQEEGEAKGQGYDKKEQYQTATRKE 594
           GAIGETIYEIAQTTKEIVIG  D          ELGQEEGE KGQ + KKE Y+TA +KE
Sbjct: 541 GAIGETIYEIAQTTKEIVIGPGDETGKQSTLGFELGQEEGEEKGQEHVKKEHYETAAKKE 491

BLAST of Lag0009693 vs. ExPASy TrEMBL
Match: A0A6J1I6Z1 (seed biotin-containing protein SBP65-like OS=Cucurbita maxima OX=3661 GN=LOC111469726 PE=4 SV=1)

HSP 1 Score: 607.8 bits (1566), Expect = 4.7e-170
Identity = 395/604 (65.40%), Postives = 427/604 (70.70%), Query Frame = 0

Query: 1   MATEQLSRRDNTTKERETHVEKERVPQLASHFEAIAVQGKGTDPSNAKATVPEKTEERQR 60
           MATE+LSRRD+TTKE+E  VE+ERVPQL SHFEAIAV GK TDPSN KAT PE+T+ RQ+
Sbjct: 1   MATEKLSRRDDTTKEKEIQVERERVPQLTSHFEAIAVHGKATDPSNEKATTPEETKARQK 60

Query: 61  SGASGGVGKEAHGTGIAKREVQGREEERQRRGGEKPEESHELATQFESLAGKVRDKRETD 120
           S AS GVGKE +GTGIA+  VQGREEERQ  GGEKPE+SHELA QFESLA KVRDKRETD
Sbjct: 61  SDAS-GVGKETYGTGIARGTVQGREEERQGVGGEKPEDSHELAAQFESLADKVRDKRETD 120

Query: 121 TENERERQARADRERTAAREREQ-GSRTREEQRASEQKVSEKEKGKEGEIGSRQNQPSLE 180
           TENERERQARAD+ER AAREREQ GSR  E+QRA EQ      K +EGEIGSRQNQPSLE
Sbjct: 121 TENERERQARADKERIAAREREQKGSRAEEKQRALEQ-----AKEREGEIGSRQNQPSLE 180

Query: 181 EISNYRAMAQEKSNEAIRAAKELYDRANKESLNQGIGARTEETGQRGTKGAERKVGEGGQ 240
           EISNYRA+AQ+KSNEAI AA                                        
Sbjct: 181 EISNYRAIAQQKSNEAIMAA---------------------------------------- 240

Query: 241 QTGLENVKERHDKANKESPNRGVGARTEETGQRGTKGAEHKVEEGGQQTGLENVKERYNK 300
                                                                 KERY+K
Sbjct: 241 ------------------------------------------------------KERYDK 300

Query: 301 ANKESLNRGVGARTEERGHRGTEEAEGKVEEGGQQTGLETVTETLTSAAKTAKDYTTPIA 360
           ANK+SL +GV A T E   RGTEEAE  VEEG Q +GLETV ETLTSAAKTAKDYT PIA
Sbjct: 301 ANKKSLEQGVEAGTREAIPRGTEEAEDTVEEGNQYSGLETVKETLTSAAKTAKDYTAPIA 360

Query: 361 EKAKDYTVQKVVEAKDMTVSAGQTTAHYLGEKAVAAKDVAIESGKVATEYAGKAAEDLKD 420
           EKAKDY           TVSAG+TTAHYLGEKAV AKDVAIESGKVA EYAGKAAEDLKD
Sbjct: 361 EKAKDY-----------TVSAGRTTAHYLGEKAVVAKDVAIESGKVAAEYAGKAAEDLKD 420

Query: 421 KAVVAGWSTAHYSCDTAMEGTKAAARLVKGAAEYAGAIAAKPLSAAKDVAESTGESLKDY 480
           K  VAGWSTAHYSCDTA+EGTKAAARLVKGAAEYAGAIAAKPLSAAK+VAESTGE++KDY
Sbjct: 421 KTAVAGWSTAHYSCDTAVEGTKAAARLVKGAAEYAGAIAAKPLSAAKNVAESTGETVKDY 480

Query: 481 TARKKAEAQRESMYKQGEKETMYEKTKETFEGGKEEVKERGRGGRGDEKRVEEGRDDGGI 540
           TARKK EAQRESMYKQ EKE+MYEKTKETFE G EEVKERG G RG+E RVEE R+DGGI
Sbjct: 481 TARKKEEAQRESMYKQTEKESMYEKTKETFEEGIEEVKERG-GERGEENRVEEDRNDGGI 492

Query: 541 LGAIGETIYEIAQTTKEIVIGASD----------ELGQEEGEAKGQGYDKKEQYQTATRK 594
           LGAIGETIYEIAQTTK+IVIG  D          ELGQEE E KGQ ++KKE Y+TA +K
Sbjct: 541 LGAIGETIYEIAQTTKDIVIGPGDETGKQSTLGFELGQEEDEEKGQEHEKKEHYETAAKK 492

BLAST of Lag0009693 vs. ExPASy TrEMBL
Match: A0A6J1JER8 (seed biotin-containing protein SBP65-like OS=Cucurbita maxima OX=3661 GN=LOC111483844 PE=4 SV=1)

HSP 1 Score: 567.8 bits (1462), Expect = 5.4e-158
Identity = 377/607 (62.11%), Postives = 404/607 (66.56%), Query Frame = 0

Query: 1   MATEQLSRRDNTTKERETHVEKERVPQLASHFEAIAVQGKGTDPSNAKATVPEKTEERQR 60
           MA EQLSRRDNTTKERE  VEKE+VPQL SHFEAIAVQ         KA  PEKT E   
Sbjct: 1   MAAEQLSRRDNTTKEREIQVEKEKVPQLTSHFEAIAVQ--------CKAATPEKTHELAS 60

Query: 61  SGASGGVGKEAHGTGIAKREVQGREEERQ----RRGGEKPEESHELATQFESLAGKVRDK 120
            G     GKEA GT IAK  + GRE  R       GG KP+++HELA QF+SLA KVRDK
Sbjct: 61  DG-----GKEAQGTSIAKGALPGREVRRSIAEGEVGGGKPDDTHELAAQFDSLAEKVRDK 120

Query: 121 RETDTENERERQARADRERTAAREREQGSRTREEQRASEQKVSEKEKGKEGEIGSRQNQP 180
           RETDTENER+R+ARAD+ER      ++GSR  EEQR SEQK     KGKEG+IGSRQNQP
Sbjct: 121 RETDTENERKRRARADKER-----EQKGSRIGEEQRLSEQK-----KGKEGDIGSRQNQP 180

Query: 181 SLEEISNYRAMAQEKSNEAIRAAKELYDRANKESLNQGIGARTEETGQRGTKGAERKVGE 240
           SLEEISN RA  QEKSN+A  AAKE YD A KESLNQG+GARTEE+ QRGT+G       
Sbjct: 181 SLEEISNVRATEQEKSNKATMAAKERYDEAKKESLNQGVGARTEESDQRGTEG------- 240

Query: 241 GGQQTGLENVKERHDKANKESPNRGVGARTEETGQRGTKGAEHKVEEGGQQTGLENVKER 300
                                                                       
Sbjct: 241 ------------------------------------------------------------ 300

Query: 301 YNKANKESLNRGVGARTEERGHRGTEEAEGKVEEGGQQTGLETVTETLTSAAKTAKDYTT 360
                                      AE  VEE GQQTGL  V ETLTSAAKTAKDYT 
Sbjct: 301 ---------------------------AEDTVEEKGQQTGLGAVEETLTSAAKTAKDYTV 360

Query: 361 PIAEKAKDYTVQKVVEAKDMTVSAGQTTAHYLGEKAVAAKDVAIESGKVATEYAGKAAED 420
           PIAEKAKDYTVQK VEAKD+TV+ GQTTAHYLGEKAVAAKDV I  GKVA EYAGKA ED
Sbjct: 361 PIAEKAKDYTVQKAVEAKDITVTVGQTTAHYLGEKAVAAKDVVIGGGKVAAEYAGKATED 420

Query: 421 LKDKAVVAGWSTAHYSCDTAMEGTKAAARLVKGAAEYAGAIAAKPLSAAKDVAESTGESL 480
           LKDKAVVAGWSTAHYSCDTA+EGTKAAARLVKGAAE AGAIAAKPLSAAK VAESTGES+
Sbjct: 421 LKDKAVVAGWSTAHYSCDTAVEGTKAAARLVKGAAEQAGAIAAKPLSAAKTVAESTGESV 480

Query: 481 KDYTARKKAEAQRESMYKQGEKETMYEKTKETFEGGKEEVKERGRGGRGDEKRVEEGRDD 540
           K+YTARKK EAQRES YKQ EKE+MYEKTKETFEG +EEVKERG  GR +EK   EGRDD
Sbjct: 481 KEYTARKKEEAQRESTYKQAEKESMYEKTKETFEGAREEVKERGE-GRVNEKNAAEGRDD 489

Query: 541 GGILGAIGETIYEIAQTTKEIVIGASDEL----------GQEEGEAKGQGYDKKEQYQTA 594
           GGILGAIGETIYEIAQTTKEIVIG  DE           G+EEGEA  QGY + E YQTA
Sbjct: 541 GGILGAIGETIYEIAQTTKEIVIGHGDETRKQNTFDFEHGEEEGEANDQGYGRGEHYQTA 489

BLAST of Lag0009693 vs. ExPASy TrEMBL
Match: A0A5A7ULM4 (Seed biotin-containing protein SBP65 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold24G002010 PE=4 SV=1)

HSP 1 Score: 562.4 bits (1448), Expect = 2.3e-156
Identity = 371/594 (62.46%), Postives = 412/594 (69.36%), Query Frame = 0

Query: 1   MATEQLSRRDNTTKERETHVEKERVPQLASHFEAIAVQGKGTDPSNAKATVPEKTEERQR 60
           MA+EQLSRRDNTT ERE  VEKERVPQL SHFEAIA QG        KAT+PEKTEE+QR
Sbjct: 1   MASEQLSRRDNTTNEREIQVEKERVPQLTSHFEAIAGQG--------KATIPEKTEEKQR 60

Query: 61  SGASGGVGKEAHGTGIAKREVQGREEERQRRGGE--KPEESHELATQFESLAGKVRDKRE 120
           SGASGG  KE HG  IAK E+ GREEER+R  GE  KP E+HELA+QFESLA KVRD RE
Sbjct: 61  SGASGG-RKEVHGISIAKGELPGREEERKRVEGEGGKPNETHELASQFESLAQKVRDNRE 120

Query: 121 TDTENERERQARADRERTAAREREQGSRTREEQRASEQKVSEKEKGKEGEIGSRQNQPSL 180
           TDTENER+RQ   DRE               EQR S+Q+ SE+ K KEGEIG+RQNQPSL
Sbjct: 121 TDTENERQRQ---DRE--------------NEQRVSKQRDSEQAKEKEGEIGTRQNQPSL 180

Query: 181 EEISNYRAMAQEKSNEAIRAAKELYDRANKESLNQGIGARTEETGQRGTKGAERKVGEGG 240
           +EISNYRA+AQE+SNEAIRAAKE Y++ANKESL+QG+  +TEE+ QR T           
Sbjct: 181 DEISNYRAIAQERSNEAIRAAKERYEKANKESLHQGVEGKTEESEQRET----------- 240

Query: 241 QQTGLENVKERHDKANKESPNRGVGARTEETGQRGTKGAEHKVEEGGQQTGLENVKERYN 300
                                                      EEG + T          
Sbjct: 241 -------------------------------------------EEGEENT---------- 300

Query: 301 KANKESLNRGVGARTEERGHRGTEEAEGKVEEGGQQTGLETVTETLTSAAKTAKDYTTPI 360
                                        VE GG++TGLETV ETLTSAAKTAKDYT PI
Sbjct: 301 -----------------------------VEVGGERTGLETVKETLTSAAKTAKDYTVPI 360

Query: 361 AEKAKDYTVQKVVEAKDMTVSAGQTTAHYLGEKAVAAKDVAIESGKVATEYAGKAAEDLK 420
           AEKAKDYTVQK +EAKD+TVSAGQTTAHYLGEKAVAAKDVA+ESGKVA EYAGKAAEDLK
Sbjct: 361 AEKAKDYTVQKAIEAKDITVSAGQTTAHYLGEKAVAAKDVAVESGKVAAEYAGKAAEDLK 420

Query: 421 DKAVVAGWSTAHYSCDTAMEGTKAAARLVKGAAEYAGAIAAKPLSAAKDVAESTGESLKD 480
           DKAVVAGWSTAHYSCDTAMEGTKAAARLVKGAAEYAGAIA KPLSAAK+VA+STGES+K+
Sbjct: 421 DKAVVAGWSTAHYSCDTAMEGTKAAARLVKGAAEYAGAIAGKPLSAAKNVAQSTGESVKE 465

Query: 481 YTARKKAEAQRESMYKQGEKETMYEKTKETFEGGKEEVKERGRGGRGDEKRVEEGRDDGG 540
           YTARKK  A+RE+ YK GEKETMYEKTK TFEGGKEE+K     GRG+EKRV+   DDGG
Sbjct: 481 YTARKKEVAEREATYKTGEKETMYEKTKGTFEGGKEELK-----GRGNEKRVD---DDGG 465

Query: 541 ILGAIGETIYEIAQTTKEIVIGASDELGQEEGEAKGQGYDKKEQYQTATRKEHR 593
           ILGAIGETIYEIAQTTKEIVIG  DE G++     G    ++EQY+T TRKE R
Sbjct: 541 ILGAIGETIYEIAQTTKEIVIGDGDESGKQ--SKLGFEIGEEEQYETDTRKEQR 465

BLAST of Lag0009693 vs. ExPASy TrEMBL
Match: A0A1S3C372 (seed biotin-containing protein SBP65 OS=Cucumis melo OX=3656 GN=LOC103496368 PE=4 SV=1)

HSP 1 Score: 562.0 bits (1447), Expect = 3.0e-156
Identity = 371/594 (62.46%), Postives = 412/594 (69.36%), Query Frame = 0

Query: 1   MATEQLSRRDNTTKERETHVEKERVPQLASHFEAIAVQGKGTDPSNAKATVPEKTEERQR 60
           MA+EQLSRRDNTT ERE  VEKERVPQL SHFEAIA QG        KAT+PEKTEE+QR
Sbjct: 1   MASEQLSRRDNTTNEREIQVEKERVPQLTSHFEAIAGQG--------KATIPEKTEEKQR 60

Query: 61  SGASGGVGKEAHGTGIAKREVQGREEERQRRGGE--KPEESHELATQFESLAGKVRDKRE 120
           SGASGG  KE HG  IAK E+ GREEER+R  GE  KP E+HELA+QFESLA KVRD RE
Sbjct: 61  SGASGG-RKEVHGISIAKGELPGREEERKRVEGEGGKPNETHELASQFESLAQKVRDNRE 120

Query: 121 TDTENERERQARADRERTAAREREQGSRTREEQRASEQKVSEKEKGKEGEIGSRQNQPSL 180
           TDTENER+RQ   DRE               EQR S+Q+ SE+ K KEGEIG+RQNQPSL
Sbjct: 121 TDTENERQRQ---DRE--------------NEQRVSKQRDSEQAKEKEGEIGTRQNQPSL 180

Query: 181 EEISNYRAMAQEKSNEAIRAAKELYDRANKESLNQGIGARTEETGQRGTKGAERKVGEGG 240
           +EISNYRA+AQE+SNEAIRAAKE Y++ANKESL+QG+  +TEE+ QR T           
Sbjct: 181 DEISNYRAIAQERSNEAIRAAKERYEKANKESLHQGVEGKTEESEQRET----------- 240

Query: 241 QQTGLENVKERHDKANKESPNRGVGARTEETGQRGTKGAEHKVEEGGQQTGLENVKERYN 300
                                                      EEG + T          
Sbjct: 241 -------------------------------------------EEGEENT---------- 300

Query: 301 KANKESLNRGVGARTEERGHRGTEEAEGKVEEGGQQTGLETVTETLTSAAKTAKDYTTPI 360
                                        VE GG++TGLETV ETLTSAAKTAKDYT PI
Sbjct: 301 -----------------------------VEVGGERTGLETVKETLTSAAKTAKDYTVPI 360

Query: 361 AEKAKDYTVQKVVEAKDMTVSAGQTTAHYLGEKAVAAKDVAIESGKVATEYAGKAAEDLK 420
           AEKAKDYTVQK +EAKD+TVSAGQTTAHYLGEKAVAAKDVA+ESGKVA EYAGKAAEDLK
Sbjct: 361 AEKAKDYTVQKAIEAKDITVSAGQTTAHYLGEKAVAAKDVAVESGKVAAEYAGKAAEDLK 420

Query: 421 DKAVVAGWSTAHYSCDTAMEGTKAAARLVKGAAEYAGAIAAKPLSAAKDVAESTGESLKD 480
           DKAVVAGWSTAHYSCDTAMEGTKAAARLVKGAAEYAGAIA KPLSAAK+VA+STGES+K+
Sbjct: 421 DKAVVAGWSTAHYSCDTAMEGTKAAARLVKGAAEYAGAIAGKPLSAAKNVAQSTGESVKE 465

Query: 481 YTARKKAEAQRESMYKQGEKETMYEKTKETFEGGKEEVKERGRGGRGDEKRVEEGRDDGG 540
           YTARKK  A+RE+ YK GEKETMYEKTK TFEGGKEE+K     GRG+EKRV+   DDGG
Sbjct: 481 YTARKKEIAEREATYKTGEKETMYEKTKGTFEGGKEELK-----GRGNEKRVD---DDGG 465

Query: 541 ILGAIGETIYEIAQTTKEIVIGASDELGQEEGEAKGQGYDKKEQYQTATRKEHR 593
           ILGAIGETIYEIAQTTKEIVIG  DE G++     G    ++EQY+T TRKE R
Sbjct: 541 ILGAIGETIYEIAQTTKEIVIGDGDESGKQ--SKLGFEIGEEEQYETDTRKEQR 465

BLAST of Lag0009693 vs. TAIR 10
Match: AT2G42560.1 (late embryogenesis abundant domain-containing protein / LEA domain-containing protein )

HSP 1 Score: 127.5 bits (319), Expect = 3.6e-29
Identity = 209/704 (29.69%), Postives = 292/704 (41.48%), Query Frame = 0

Query: 1   MATEQLSRRDNTTKERETHVEKERVPQLASHFEAIAVQGKGTDPSNAKATVPEKTEERQR 60
           MA+EQ +RR+N   ERE  VEK+RVP++ SHFE++A +GK +D           T   Q 
Sbjct: 1   MASEQ-ARRENKVTEREVQVEKDRVPKMTSHFESMAEKGKDSD-----------THRHQT 60

Query: 61  SGASGGVGKEAHGTGIAKREVQGREEERQRRGGEKPEESHELATQFESLAGK-----VRD 120
            G                             GG          TQF SL+ K     V D
Sbjct: 61  EG-----------------------------GG----------TQFVSLSDKGSNMPVSD 120

Query: 121 KRETDTENERERQARADRERTAAREREQGSRTREEQRASEQKVSEKEKGKEGEIGSRQNQ 180
           + E +T+ +R +   +            G         + +K  EKE+           +
Sbjct: 121 EGEGETKMKRTQMPHS-----------VGKFVTSSDSGTGKKKDEKEE---------HEK 180

Query: 181 PSLEEISNYRAMAQEKSNEAIRAAKELYDRANKESLN----QGIGARTEETGQRGTKGAE 240
            SLE+I  YRA AQ+KS ++I+AA+E Y++A KESL+    +  G R EE   +G     
Sbjct: 181 ASLEDIHGYRANAQQKSMDSIKAAEERYNKA-KESLSHSGQEARGGRGEEMVGKGRDSGV 240

Query: 241 RKV-------GEGGQQT-----GLENVKERH------------DKANKESPNRGVGART- 300
           R         G GG++      G    K RH            ++  KES   GVG R+ 
Sbjct: 241 RVSHVGAVGGGGGGEEKESGVHGFHGEKARHAELLAAGGEEMREREGKESAG-GVGGRSV 300

Query: 301 EETGQRGTKGAEHKVEEGGQQTGLENVKERYNKANKESLNRG--VGARTEERGHRGTEEA 360
           ++T     + A+  V EG Q+ G     E+  +A++ +  +G   G  T E+  R  + A
Sbjct: 301 KDTVAEKGQQAKESVGEGAQKAG-SATSEKAQRASEYATEKGKEAGNMTAEQAARAKDYA 360

Query: 361 EGKVEEGGQQTGLETVTETLTSAAKTAKDYTTPIAE---KAKDYTVQKVVEAKDMTVSAG 420
             K  E       ET  E    A++  K+  +  AE   +AKDYT+QK VEAKD+     
Sbjct: 361 LQKAVE-----AKETAAEKAQRASEYMKETGSTAAEQAARAKDYTLQKAVEAKDVAAEKA 420

Query: 421 QTTAHYL---------------------------------GEKAVAAKDVAIESGKVATE 480
           Q  + Y+                                 GE A   K+  +E GK A  
Sbjct: 421 QRASEYMTETGKQAGNVAAQKGQEAASMTAKAKDYTVQKAGEAAGYIKETTVEGGKGAAH 480

Query: 481 YAGKAAEDLKDKAVVAGWSTAHYSCDTAMEGTKAAARLVKGAAEYAG----AIAAKPLSA 540
           YAG AAE    KA   GW+ AH++ +  ++GTKA A  V+GA  YAG     + +K +  
Sbjct: 481 YAGVAAE----KAAAVGWTAAHFTTEKVVQGTKAVAGTVEGAVGYAGHKAVEVGSKAVDL 540

Query: 541 AKDVAESTGESLKDYTARKKAEAQR----------------------------------- 571
            K+ A    +++  YTARKK EAQ                                    
Sbjct: 541 TKEKAAVAADTVVGYTARKKEEAQHRDQEMHQGGEEEKQPGFVSGARRDFGEEYGEERGS 600

BLAST of Lag0009693 vs. TAIR 10
Match: AT2G36640.1 (embryonic cell protein 63 )

HSP 1 Score: 63.5 bits (153), Expect = 6.4e-10
Identity = 87/287 (30.31%), Postives = 118/287 (41.11%), Query Frame = 0

Query: 321 TEEAEGKVEEGGQQTGLETV--TETLTSAAKTAKDYTTPIAEKAKDYTVQKVVEAKDMTV 380
           T EA    +EG Q    + V   +     AK   DYT     + KDYTV K  EAKD T 
Sbjct: 77  THEAAESTKEGAQIASEKAVGAKDATVEKAKETADYTAEKVGEYKDYTVDKAKEAKDTTA 136

Query: 381 SAGQTTAHYLGEKAVAAKDVAIESGKVATEYAGKAAEDLKDKAVVAGWSTAHYSCDTAME 440
              + TA+Y  +KAV AKD   E      +YA   A + KDK       TA+Y+ D A E
Sbjct: 137 EKAKETANYTADKAVEAKDKTAEKIGEYKDYAVDKAVEAKDKTAEKAKETANYTADKAKE 196

Query: 441 GTKAAAR--------LVKGAAEYAGAIAAKPLSAAKDVAESTGESLKDYTARKKAEAQRE 500
                A          V  A E     A K + A    AE TGE  KDYT  K  E +  
Sbjct: 197 AKDKTAEKVGEYKDYTVDKAVEARDYTAEKAIEAKDKTAEKTGE-YKDYTVEKATEGKDV 256

Query: 501 SMYKQGE-KETMYEKTKET---FEGGKEEVKERG------------RGGRGDEKRVEEGR 560
           ++ K GE K++  E  K       G  EE K +             + G    +++EE R
Sbjct: 257 TVSKLGELKDSAVETAKRAMGFLSGKTEEAKGKAVETKDTAKENMEKAGEVTRQKMEEMR 316

Query: 561 DDGGIL-GAIGETIYEIAQTTKEIVIGASDELGQEEGEAKGQGYDKK 581
            +G  L    G    E +Q T+E     + +  + +  A  +G + K
Sbjct: 317 LEGKELKEEAGAKAQEASQKTRESTESGAQKAEETKDSAAVRGNEAK 362

BLAST of Lag0009693 vs. TAIR 10
Match: AT3G53040.1 (late embryogenesis abundant protein, putative / LEA protein, putative )

HSP 1 Score: 52.4 bits (124), Expect = 1.5e-06
Identity = 89/309 (28.80%), Postives = 120/309 (38.83%), Query Frame = 0

Query: 303 ESLNRGVGARTEERGHRGTEEAEGKVEEGGQQTGLETVTETLTSAAKTAKDYTTPIAEKA 362
           ES   G    +E+    G  +  G+V +   Q   ET   T    A+ AKD T    ++ 
Sbjct: 78  ESTREGADIASEKAA--GMRDTTGEVRDSTAQKTKETADYT-ADKAREAKDKTADKTKET 137

Query: 363 KDYTVQKVVEAKDMTVSAGQTTAHYLGEKAVAAKDVAIESGKVATEYAGKAAEDLKDKAV 422
            DY  +K  EAKD T    + TA Y  EKA  AKD   +      +Y  + A++ KD   
Sbjct: 138 ADYAAEKAREAKDRTADKTKETAEYTAEKAREAKDKTADKLGEYKDYTAEKAKEAKDTTA 197

Query: 423 VAGWSTAHYSCDTAMEGTKAAARLVKGAAEYAGAIAAKPLSAAKDVAESTGESLKDYTAR 482
                   Y+ D A E     A   K  AEY    + K        AE  GE  KDYTA 
Sbjct: 198 EKLGEYKDYTVDKAKEAKDKTAEKAKETAEYT---SDKARETKDKTAEKVGE-YKDYTAE 257

Query: 483 K---KAEAQRESMYKQGEKETMY-----EKTKETFEGGKEEVKERGRGGRGDEKRVEEGR 542
           K    A+  RE+  K  EK   Y     EK  ET + G  ++      G   +  V+  +
Sbjct: 258 KAKETADKAREAKDKTAEKVGEYRDYTAEKATETKDAGVSKI------GELKDSAVDTAK 317

Query: 543 DDGGILGAIGETIYEIAQTTKEIVIGASDELGQE----------EGEAKGQGYDKKEQYQ 592
              G L    E   + A  TK+      DE G+E          EG+   +   +K Q  
Sbjct: 318 RAMGFLSGKTEETKQKAVETKDTAKEKMDEAGEEARRKMEEMRLEGKKLDEDASRKTQQS 373