Lag0009661 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0009661
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	transcription factor bHLH162-like
Location	chr9: 41215049 .. 41216092 (+)
RNA-Seq Expression	Lag0009661
Synteny	Lag0009661

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGGGTTCTCGTGAAGGGAATATTCAACAAAATGTTGGTTCTATTAAGGTCGAAAGAAAGGTTATGGAGAAGAATCGTAGAACCCAGATGAAATTGCTTTACTCTAAACTCAATTCTCTCCTCCCAACCCATCACTCCAATGTTCGTTTCTTTATGTTCTTTCAACTCAGTTCTTAATTGGGTCTCCACTAAAATTTTCAAATTTTAATTTGGTGTGGATTTCCAGGAGCTGCCACTGACAGTGTCGGATCAGATCGACGAAGCCATAAAGTACATAAAATCACTCGAGACCAAGCTGGAGAAGGATAAGGAGAAGAAGGAGAGCTTTTTGAGAAGATTGAAAAGGTCGTTGTCGACGTCGTCGTCGTCGCAGCACACGGTGCCGACGAGGAGCCAGAATTGTAACTCACCTGAACTGAAAATCAAAGAGATGGGTTCGGCCGTGGAGGTTGTTTTAACATGTGGGTTGGAAGATCAGTTCTTATTTTACGAGATTATTCGCATCTTTCGTGAGGAGCGAGTCGAGATCATCAATGTCAGTTATTCTGTTCTCGAGAATACCGTCTTGTATTCACTCCATGCAGAGGTATATCGTAAGAAAAATACTTTGGTTATCTTCCTATACATCACCCTTAAGATGTTGACAATTAGGAGAGATAAAATAAAATTTTCATTCTTTACTTCTTTCTTAATTGTCAGGAATTTAATAGAGTGTGTAAAAAAATACCGATACCTACACATCGATATGCAACAAAGTGTTGTTTATATCGTAATCTATTAGTTTAAGCTTTTGAATTCGATATAATAGTTTAATAGGGTTATAAATGGTTTTTGTTTTGGATTATGAGAGAGTAGATTGAAGACGTGGTGTATGAATTTGGAGCAAAGAAACTAACGGAGAGGCTAAGAAGATTAGTTTGCGAATCGAAGAGTGATGAAGAAATGCAAGCAGGAGCTTCTTCTTCGAGCGGTCATGGGGGAACCGATCTGCCGGCGAACACTGCGACGACGAGTTCCGATCGCCATTTTTGGAGCCACTAG

mRNA sequence

ATGGAGGGTTCTCGTGAAGGGAATATTCAACAAAATGTTGGTTCTATTAAGGTCGAAAGAAAGGTTATGGAGAAGAATCGTAGAACCCAGATGAAATTGCTTTACTCTAAACTCAATTCTCTCCTCCCAACCCATCACTCCAATGAGCTGCCACTGACAGTGTCGGATCAGATCGACGAAGCCATAAAGTACATAAAATCACTCGAGACCAAGCTGGAGAAGGATAAGGAGAAGAAGGAGAGCTTTTTGAGAAGATTGAAAAGGTCGTTGTCGACGTCGTCGTCGTCGCAGCACACGGTGCCGACGAGGAGCCAGAATTGTAACTCACCTGAACTGAAAATCAAAGAGATGGGTTCGGCCGTGGAGGTTGTTTTAACATGTGGGTTGGAAGATCAGTTCTTATTTTACGAGATTATTCGCATCTTTCGTGAGGAGCGAGTCGAGATCATCAATGTCAGTTATTCTGTTCTCGAGAATACCGTCTTGTATTCACTCCATGCAGAGATTGAAGACGTGGTGTATGAATTTGGAGCAAAGAAACTAACGGAGAGGCTAAGAAGATTAGTTTGCGAATCGAAGAGTGATGAAGAAATGCAAGCAGGAGCTTCTTCTTCGAGCGGTCATGGGGGAACCGATCTGCCGGCGAACACTGCGACGACGAGTTCCGATCGCCATTTTTGGAGCCACTAG

Coding sequence (CDS)

ATGGAGGGTTCTCGTGAAGGGAATATTCAACAAAATGTTGGTTCTATTAAGGTCGAAAGAAAGGTTATGGAGAAGAATCGTAGAACCCAGATGAAATTGCTTTACTCTAAACTCAATTCTCTCCTCCCAACCCATCACTCCAATGAGCTGCCACTGACAGTGTCGGATCAGATCGACGAAGCCATAAAGTACATAAAATCACTCGAGACCAAGCTGGAGAAGGATAAGGAGAAGAAGGAGAGCTTTTTGAGAAGATTGAAAAGGTCGTTGTCGACGTCGTCGTCGTCGCAGCACACGGTGCCGACGAGGAGCCAGAATTGTAACTCACCTGAACTGAAAATCAAAGAGATGGGTTCGGCCGTGGAGGTTGTTTTAACATGTGGGTTGGAAGATCAGTTCTTATTTTACGAGATTATTCGCATCTTTCGTGAGGAGCGAGTCGAGATCATCAATGTCAGTTATTCTGTTCTCGAGAATACCGTCTTGTATTCACTCCATGCAGAGATTGAAGACGTGGTGTATGAATTTGGAGCAAAGAAACTAACGGAGAGGCTAAGAAGATTAGTTTGCGAATCGAAGAGTGATGAAGAAATGCAAGCAGGAGCTTCTTCTTCGAGCGGTCATGGGGGAACCGATCTGCCGGCGAACACTGCGACGACGAGTTCCGATCGCCATTTTTGGAGCCACTAG

Protein sequence

MEGSREGNIQQNVGSIKVERKVMEKNRRTQMKLLYSKLNSLLPTHHSNELPLTVSDQIDEAIKYIKSLETKLEKDKEKKESFLRRLKRSLSTSSSSQHTVPTRSQNCNSPELKIKEMGSAVEVVLTCGLEDQFLFYEIIRIFREERVEIINVSYSVLENTVLYSLHAEIEDVVYEFGAKKLTERLRRLVCESKSDEEMQAGASSSSGHGGTDLPANTATTSSDRHFWSH

Homology

[Close]

BLAST of Lag0009661 vs. NCBI nr
Match: XP_038902351.1 (transcription factor bHLH162-like [Benincasa hispida])

HSP 1 Score: 266.5 bits (680), Expect = 2.1e-67
Identity = 157/229 (68.56%), Postives = 179/229 (78.17%), Query Frame = 0


Query: 1   MEGSREGNIQQNVGSIKVERKVMEKNRRTQMKLLYSKLNSLLPTHHSNELPLTVSDQIDE 60
           M  +RE N Q N    KVERKVMEKNRR +MK LY+ LNSLLP  HSNELPLTV DQID+
Sbjct: 1   MAENRELNSQNN----KVERKVMEKNRRNKMKSLYTNLNSLLPISHSNELPLTVLDQIDK 60

Query: 61  AIKYIKSLETKLEKDKEKKESFLRRLKRSLSTSSSSQHTVPTRSQNCNSPELKIKEMGSA 120
           AIKYIKSLE  L+KD+EKKE  LR+ K S   SSSS +T  + S N N PELKIKEMGSA
Sbjct: 61  AIKYIKSLEINLKKDEEKKERLLRKSKLS---SSSSSYTALSMSPNRNVPELKIKEMGSA 120

Query: 121 VEVVLTCGLEDQFLFYEIIRIFREERVEIINVSYSVLENTVLYSLHAEIEDVVYEFGAKK 180
           VEVVLT GLED+ +FYEIIRIF EERVEIIN+SYS+LENT++YSLH EIEDVVYEFG  K
Sbjct: 121 VEVVLTTGLEDRSIFYEIIRIFDEERVEIINISYSILENTIIYSLHTEIEDVVYEFGGTK 180

Query: 181 LTERLRRLVCESKSDEEMQAGASSSSGHGGTDLPANTATTSSDRHFWSH 230
           L ERL++LV E  +D E+QA A SSS HGGT+ PA+  T SSD +FWSH
Sbjct: 181 LIERLKKLVHEPNNDPEIQAVAVSSS-HGGTNFPADVPTMSSDYNFWSH 221

[Close]

BLAST of Lag0009661 vs. NCBI nr
Match: XP_038902350.1 (transcription factor bHLH162-like [Benincasa hispida])

HSP 1 Score: 250.8 bits (639), Expect = 1.2e-62
Identity = 147/213 (69.01%), Postives = 172/213 (80.75%), Query Frame = 0


Query: 17  KVERKVMEKNRRTQMKLLYSKLNSLLPTHHSNELPLTVSDQIDEAIKYIKSLETKLEKDK 76
           K+ERKV+EKNRR +MK LYS LNSLLPT HSN LPLTVS+QI+E IK IKSLE KL+KD+
Sbjct: 87  KIERKVIEKNRRNKMKSLYSNLNSLLPTPHSN-LPLTVSEQIEEVIKSIKSLEIKLKKDE 146

Query: 77  EKKESFLRRLKRSLSTSSSSQHTVPTRSQNCNSPELKIKEMGSAVEVVLTCGLEDQFLFY 136
           EKKE  LR+ K SLS+ S   +T  + +QN N PELKIKEMGSAVEVVLT GLED+ +FY
Sbjct: 147 EKKERLLRKSKMSLSSPS---YTALSTNQNRNVPELKIKEMGSAVEVVLTTGLEDRSIFY 206

Query: 137 EIIRIFREERVEIINVSYSVLENTVLYSLHAEIEDVVYEFGAKKLTERLRRLVCESKSDE 196
           EIIRIF EERVEIINVSYS LE+T++YSLHAEIEDVVYEFG  KL ERL++LV E  +D 
Sbjct: 207 EIIRIFNEERVEIINVSYSDLESTIIYSLHAEIEDVVYEFGGTKLIERLKKLVHEPNNDP 266

Query: 197 EMQAGASSSSGHGGTDLPANTATTSSDRHFWSH 230
           E+QA A  SS HGGT+ PA+  TTSS  +FWS+
Sbjct: 267 ELQAVAVFSS-HGGTNFPADVLTTSSHYNFWSN 294

[Close]

BLAST of Lag0009661 vs. NCBI nr
Match: KAG6604789.1 (Transcription factor basic helix-loop-helix 162, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 246.5 bits (628), Expect = 2.2e-61
Identity = 150/229 (65.50%), Postives = 166/229 (72.49%), Query Frame = 0


Query: 1   MEGSREGNIQQN---VGSIKVERKVMEKNRRTQMKLLYSKLNSLLPTHHSNELPLTVSDQ 60
           ME +RE   QQN   V + KVERKVMEKNRR QMKLLYS LNSLLP + S + PLTVSDQ
Sbjct: 1   MESNREAGNQQNQQDVNTNKVERKVMEKNRRNQMKLLYSNLNSLLPPNKSTDSPLTVSDQ 60

Query: 61  IDEAIKYIKSLETKLEKDKEKKESFLRRLKRSLSTSSSSQHTVPTRSQNCNSPELKIKEM 120
           I+EAIKYIKSLE KL+K  EKKE  L RL  S + S  +       S N NSPEL+IKEM
Sbjct: 61  IEEAIKYIKSLEMKLQKCMEKKERILARLNMSSALSDGA-------STNNNSPELRIKEM 120

Query: 121 GSAVEVVLTCGLEDQFLFYEIIRIFREERVEIINVSYSVLENTVLYSLHAEIEDVVYEFG 180
           GS VEVV T G EDQ LF E+I +F EER EIIN++YSV EN  LYS+HAEIEDVVYE G
Sbjct: 121 GSIVEVVFTRGFEDQPLFDELIGVFHEERAEIINLNYSVHENMFLYSIHAEIEDVVYELG 180

Query: 181 AKKLTERLRRLVCESKSDEEMQAGASSSSGHGGTDLPANTATTSSDRHF 227
           AKKL ERL +LV E KSD EM AG +SSSGHGGT  PAN  T SS  HF
Sbjct: 181 AKKLVERLNKLVYEWKSDAEMVAGGASSSGHGGTHPPANIPTMSSGSHF 222

[Close]

BLAST of Lag0009661 vs. NCBI nr
Match: XP_023512282.1 (transcription factor bHLH162-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 242.7 bits (618), Expect = 3.2e-60
Identity = 137/196 (69.90%), Postives = 158/196 (80.61%), Query Frame = 0


Query: 31  MKLLYSKLNSLLPTHHSNELPLTVSDQIDEAIKYIKSLETKLEKDKEKKESFLRRLKRSL 90
           MKLLYSKLNSLLPTHHSNELPL+VSDQI+EAIKYIKSLE KL+KD+EKKE F RR     
Sbjct: 1   MKLLYSKLNSLLPTHHSNELPLSVSDQIEEAIKYIKSLELKLKKDEEKKERFTRR----- 60

Query: 91  STSSSSQHTVPTRSQNCNSPELKIKEMGSAVEVVLTCGLEDQFLFYEIIRIFREERVEII 150
            +SSSS +  PTR++N N PEL+IKEMGS VEVVL+ GLED+F+FYEII IF EER EI+
Sbjct: 61  -SSSSSSYMAPTRTRNRNGPELRIKEMGSVVEVVLSTGLEDRFIFYEIIHIFYEERAEIV 120

Query: 151 NVSYSVLENTVLYSLHAEIEDVVYEFGAKKLTERLRRLVCESKSDEEMQAGASSSSGHGG 210
           NVSYSV+ N+VLYSLHAEIEDVVYEFGA K TER++RLV   ++D EM+A A SSS HGG
Sbjct: 121 NVSYSVVGNSVLYSLHAEIEDVVYEFGATKPTERIKRLVYGWENDAEMKA-AGSSSSHGG 180

Query: 211 TDLPANTATTSSDRHF 227
              P N  +T SD  F
Sbjct: 181 IRRPGNGPSTCSDNQF 189

[Close]

BLAST of Lag0009661 vs. NCBI nr
Match: XP_022944108.1 (transcription factor bHLH162-like [Cucurbita moschata])

HSP 1 Score: 238.0 bits (606), Expect = 7.8e-59
Identity = 138/200 (69.00%), Postives = 159/200 (79.50%), Query Frame = 0


Query: 31  MKLLYSKLNSLLPTHHSNELPLTVSDQIDEAIKYIKSLETKLEKDKEKKESFLRRLKRSL 90
           MK LYSKLNSLLPTHHSNELPL+V DQI+EAIKYIKSLE KL+KD+EKKE F RR     
Sbjct: 1   MKSLYSKLNSLLPTHHSNELPLSVLDQIEEAIKYIKSLELKLKKDEEKKERFTRR----- 60

Query: 91  STSSSSQHTVPTRSQNCNSPELKIKEMGSAVEVVLTCGL-EDQFLFYEIIRIFREERVEI 150
             SSSS +  PTRS+N N PEL+IKEMGSAVEVVL+ GL ED+F+FYEII IF EER EI
Sbjct: 61  --SSSSSYMAPTRSRNRNGPELRIKEMGSAVEVVLSTGLEEDRFIFYEIIHIFYEERAEI 120

Query: 151 INVSYSVLENTVLYSLHAEIEDVVYEFGAKKLTERLRRLVCESKSDEEMQAGASSSSGHG 210
           +NVSYSV+ N+VLYSL+AEIEDVVYEFGA K TER++RLV   ++D EM+A A SSS HG
Sbjct: 121 VNVSYSVVGNSVLYSLYAEIEDVVYEFGATKPTERIKRLVYGWENDAEMKA-AGSSSSHG 180

Query: 211 GTDLPANTATTSSDRHFWSH 230
           G   P N  +T SD  F+SH
Sbjct: 181 GIRRPGNGPSTCSDNQFYSH 192

[Close]

BLAST of Lag0009661 vs. ExPASy Swiss-Prot
Match: F4JIJ7 (Transcription factor bHLH162 OS=Arabidopsis thaliana OX=3702 GN=BHLH162 PE=1 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 4.5e-25
Identity = 75/179 (41.90%), Postives = 116/179 (64.80%), Query Frame = 0


Query: 15  SIKVERKVMEKNRRTQMKLLYSKLNSLLPTHHSNELPLTVSDQIDEAIKYIKSLETKLEK 74
           S  V+RK +EKNRR QMK LYS+L SLLP HHS+  PLT+ DQ+DEA  YIK L+  +EK
Sbjct: 11  SRSVDRKTVEKNRRMQMKSLYSELISLLP-HHSSTEPLTLPDQLDEAANYIKKLQVNVEK 70

Query: 75  DKEKKESFL--RRLKRSLSTSSSSQHTVPTRSQNCNSPELKIKEMGSAVEVVLTCGLEDQ 134
            +E+K + +    L++  S  SSS  +    S     P+++I+E GS   + L   LE +
Sbjct: 71  KRERKRNLVATTTLEKLNSVGSSSVSSSVDVSVPRKLPKIEIQETGSIFHIFLVTSLEHK 130

Query: 135 FLFYEIIRIFREE-RVEIINVSYSVLENTVLYSLHAEIEDVVYEFGAK-KLTERLRRLV 190
           F+F EIIR+  EE   EI +  YS++++ V ++LH ++E+  +++GA+ ++ ERL ++V
Sbjct: 131 FMFCEIIRVLTEELGAEITHAGYSIVDDAVFHTLHCKVEE--HDYGARSQIPERLEKIV 186

[Close]

BLAST of Lag0009661 vs. ExPASy Swiss-Prot
Match: Q9FLI1 (Transcription factor bHLH36 OS=Arabidopsis thaliana OX=3702 GN=BHLH36 PE=2 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 1.6e-06
Identity = 46/175 (26.29%), Postives = 92/175 (52.57%), Query Frame = 0


Query: 17  KVERKVMEKNRRTQMKLLYSKLNSLLPTHHSNELPLTVSDQIDEAIKYIKSLETKLEKDK 76
           K+  +  E+ RR +M  LY+ L SLLP H       T SDQ++EA+ YIK L+ K+++  
Sbjct: 3   KMMHRETERQRRQEMASLYASLRSLLPLHFIKGKRST-SDQVNEAVNYIKYLQRKIKELS 62

Query: 77  EKKESFLRRLKRSLSTSSSSQHTVPTRSQNCNSPELKIKEMGSAVEVVLT---CGLEDQF 136
            +++  +   + SL  SS+          +     + +++    VE++L+   CG   Q 
Sbjct: 63  VRRDDLMVLSRGSLLGSSNGDFKEDVEMIS-GKNHVVVRQCLVGVEIMLSSRCCG--GQP 122

Query: 137 LFYEIIRIFREERVEIINVSYSVLENTVLYSLHAEIEDVVYEFGAKKLTERLRRL 189
            F  ++++  E  + ++N   S++++ ++Y++ AE+ D+       +L +RL R+
Sbjct: 123 RFSSVLQVLSEYGLCLLNSISSIVDDRLVYTIQAEVNDMALMIDLAELEKRLIRM 173

[Close]

BLAST of Lag0009661 vs. ExPASy Swiss-Prot
Match: Q9XIJ1 (Transcription factor bHLH168 OS=Arabidopsis thaliana OX=3702 GN=BHLH168 PE=3 SV=1)

HSP 1 Score: 50.1 bits (118), Expect = 4.0e-05
Identity = 48/191 (25.13%), Postives = 95/191 (49.74%), Query Frame = 0


Query: 1   MEGSREGNIQQNVGSIKVERKVMEKNRRTQMKLLYSKLNSLLPTHHSNELPLTVSDQIDE 60
           ME +RE   + +  S++ +R + EK RR +MK L+    S+L +H S    L V   ID+
Sbjct: 1   MERAREIG-EGSASSLREQRNLREKERRMRMKHLF----SILSSHVSPTRRLPVPQLIDQ 60

Query: 61  AIKYIKSLETKLEKDKEKKESFLRRLKRSLSTSSSSQHTVPTRSQNCN-SPELKIKEMGS 120
           A+ Y+  L+ K+        ++L  +KR +         V  RS+  +  P+L I+ + S
Sbjct: 61  AVSYMIQLKEKV--------NYLNEMKRRMLGGE-----VKNRSEGSSLLPKLSIRSLDS 120

Query: 121 AVEVVLTCGLEDQ-FLFYEIIRIFREERVEIINVSYSVLENTVLYSLHAEIEDVVYEFGA 180
            +E+ L   L  +  + ++++ +F EE  ++++ +   L +   Y++ A+          
Sbjct: 121 IIEMNLVMDLNMKGVMLHKLVSVFEEEGAQVMSANLQNLNDRTFYTIIAQAIICRIGIDP 173

Query: 181 KKLTERLRRLV 190
            ++ ERLR ++
Sbjct: 181 SRIEERLRDII 173

[Close]

BLAST of Lag0009661 vs. ExPASy TrEMBL
Match: A0A6J1FXP8 (transcription factor bHLH162-like OS=Cucurbita moschata OX=3662 GN=LOC111448656 PE=4 SV=1)

HSP 1 Score: 238.0 bits (606), Expect = 3.8e-59
Identity = 138/200 (69.00%), Postives = 159/200 (79.50%), Query Frame = 0


Query: 31  MKLLYSKLNSLLPTHHSNELPLTVSDQIDEAIKYIKSLETKLEKDKEKKESFLRRLKRSL 90
           MK LYSKLNSLLPTHHSNELPL+V DQI+EAIKYIKSLE KL+KD+EKKE F RR     
Sbjct: 1   MKSLYSKLNSLLPTHHSNELPLSVLDQIEEAIKYIKSLELKLKKDEEKKERFTRR----- 60

Query: 91  STSSSSQHTVPTRSQNCNSPELKIKEMGSAVEVVLTCGL-EDQFLFYEIIRIFREERVEI 150
             SSSS +  PTRS+N N PEL+IKEMGSAVEVVL+ GL ED+F+FYEII IF EER EI
Sbjct: 61  --SSSSSYMAPTRSRNRNGPELRIKEMGSAVEVVLSTGLEEDRFIFYEIIHIFYEERAEI 120

Query: 151 INVSYSVLENTVLYSLHAEIEDVVYEFGAKKLTERLRRLVCESKSDEEMQAGASSSSGHG 210
           +NVSYSV+ N+VLYSL+AEIEDVVYEFGA K TER++RLV   ++D EM+A A SSS HG
Sbjct: 121 VNVSYSVVGNSVLYSLYAEIEDVVYEFGATKPTERIKRLVYGWENDAEMKA-AGSSSSHG 180

Query: 211 GTDLPANTATTSSDRHFWSH 230
           G   P N  +T SD  F+SH
Sbjct: 181 GIRRPGNGPSTCSDNQFYSH 192

[Close]

BLAST of Lag0009661 vs. ExPASy TrEMBL
Match: A0A6J1G8B1 (transcription factor bHLH162-like OS=Cucurbita moschata OX=3662 GN=LOC111451747 PE=4 SV=1)

HSP 1 Score: 232.3 bits (591), Expect = 2.1e-57
Identity = 141/221 (63.80%), Postives = 161/221 (72.85%), Query Frame = 0


Query: 1   MEGSRE--GNIQQNVGSIKVERKVMEKNRRTQMKLLYSKLNSLLPTHHSNELPLTVSDQI 60
           ME  RE  GN QQN  + KVERKV+EKNRR QMKLLYSKLNSLLP + S + PLTV DQI
Sbjct: 1   MERGREAAGN-QQNGSTNKVERKVLEKNRRNQMKLLYSKLNSLLPPNQSTDSPLTVLDQI 60

Query: 61  DEAIKYIKSLETKLEKDKEKKESFLRRLKRSLSTSSSSQHTVPTRSQNCNSPELKIKEMG 120
           +EAIKYI+SL  KL+K KEKK+  L               + PTRS + N PELKIKEMG
Sbjct: 61  EEAIKYIQSLTVKLDKVKEKKKRILM--------------SAPTRSPSSNLPELKIKEMG 120

Query: 121 SAVEVVLTCGLEDQFLFYEIIRIFREERVEIINVSYSVLENTVLYSLHAEIEDVVYEFGA 180
           SAVEVV    L D+FLFYEIIRIF EER EIIN +YSVL++TVLYSLHAEIEDV+Y FGA
Sbjct: 121 SAVEVVFMTELNDRFLFYEIIRIFHEERAEIINATYSVLDDTVLYSLHAEIEDVMYAFGA 180

Query: 181 KKLTERLRRLVCESKSDEEMQAGASSSSGHGGTDLPANTAT 220
            KLTERL+RL  E KSD E QA  +SSSG+GG+    +T T
Sbjct: 181 TKLTERLKRLAYEWKSDAEQQAAGASSSGYGGSHPSEDTPT 206

[Close]

BLAST of Lag0009661 vs. ExPASy TrEMBL
Match: A0A6J1G8Q1 (transcription factor bHLH162-like OS=Cucurbita moschata OX=3662 GN=LOC111451748 PE=4 SV=1)

HSP 1 Score: 229.9 bits (585), Expect = 1.0e-56
Identity = 135/204 (66.18%), Postives = 152/204 (74.51%), Query Frame = 0


Query: 23  MEKNRRTQMKLLYSKLNSLLPTHHSNELPLTVSDQIDEAIKYIKSLETKLEKDKEKKESF 82
           MEKNRR QMKLLYS LNSLLP + S + PLTVSDQI+EAIKYIKSLE KL+K KEKKE  
Sbjct: 1   MEKNRRNQMKLLYSNLNSLLPPNQSTDSPLTVSDQIEEAIKYIKSLEMKLQKCKEKKERI 60

Query: 83  LRRLKRSLSTSSSSQHTVPTRSQNCNSPELKIKEMGSAVEVVLTCGLEDQFLFYEIIRIF 142
           L RL  S + S  +       S N NSPEL+IKEMGS VEVV T G ED+ LF E+I +F
Sbjct: 61  LARLNMSSALSDRA-------STNNNSPELRIKEMGSIVEVVFTRGFEDRSLFDELIGVF 120

Query: 143 REERVEIINVSYSVLENTVLYSLHAEIEDVVYEFGAKKLTERLRRLVCESKSDEEMQAGA 202
            EER EIIN++YSV E+  LYS+HA+IEDVVYE GAKKL ERL +LV E KSD EM AG 
Sbjct: 121 HEERAEIINLNYSVHEDMFLYSIHAQIEDVVYELGAKKLVERLNKLVYEWKSDAEMLAGG 180

Query: 203 SSSSGHGGTDLPANTATTSSDRHF 227
           +SSSGHGGT  PAN  T SS RHF
Sbjct: 181 ASSSGHGGTHPPANIPTMSSGRHF 197

[Close]

BLAST of Lag0009661 vs. ExPASy TrEMBL
Match: A0A6J1JF05 (transcription factor bHLH162-like OS=Cucurbita maxima OX=3661 GN=LOC111483908 PE=4 SV=1)

HSP 1 Score: 228.4 bits (581), Expect = 3.0e-56
Identity = 133/197 (67.51%), Postives = 157/197 (79.70%), Query Frame = 0


Query: 31  MKLLYSKLNSLLPTHHSNELPLTVSDQIDEAIKYIKSLETKLEKDKEKKESFLRRLKRSL 90
           MK LYSKLNSLLPTHHSNE+PL+VSDQI+EAIKYIKSLE KL+K++EKKE F R+     
Sbjct: 1   MKSLYSKLNSLLPTHHSNEMPLSVSDQIEEAIKYIKSLELKLKKNEEKKERFTRQ----- 60

Query: 91  STSSSSQHTVPTRSQNCNSPELKIKEMGSAVEVVLTCGL-EDQFLFYEIIRIFREERVEI 150
             SSSS +T PTRS+N N PEL+IKE+GSAVEVVL+ GL ED+F+FYEII IF +ER EI
Sbjct: 61  --SSSSLYTAPTRSRNRNGPELRIKEIGSAVEVVLSIGLEEDRFIFYEIIHIFYQERAEI 120

Query: 151 INVSYSVLENTVLYSLHAEIEDVVYEFGAKKLTERLRRLVCESKSDEEMQAGASSSSGHG 210
           +NVSYSV+ N+VLYSLHAEIEDVVYEFGA K TER++RLV    +D EM+A A SSS  G
Sbjct: 121 VNVSYSVVGNSVLYSLHAEIEDVVYEFGATKPTERIKRLVYGWANDGEMKA-AGSSSSFG 180

Query: 211 GTDLPANTATTSSDRHF 227
           G+  P N  +T SD  F
Sbjct: 181 GSRRPGNGPSTCSDNQF 189

[Close]

BLAST of Lag0009661 vs. ExPASy TrEMBL
Match: A0A6J1I4R7 (transcription factor bHLH162-like OS=Cucurbita maxima OX=3661 GN=LOC111469622 PE=4 SV=1)

HSP 1 Score: 227.3 bits (578), Expect = 6.7e-56
Identity = 133/204 (65.20%), Postives = 151/204 (74.02%), Query Frame = 0


Query: 23  MEKNRRTQMKLLYSKLNSLLPTHHSNELPLTVSDQIDEAIKYIKSLETKLEKDKEKKESF 82
           MEKNRR QMKLLYS LNSLLP + S + PLTVSDQI+EAIKYIKSLE KL+K KEKKE  
Sbjct: 1   MEKNRRNQMKLLYSNLNSLLPPNKSTDSPLTVSDQIEEAIKYIKSLEMKLQKCKEKKERI 60

Query: 83  LRRLKRSLSTSSSSQHTVPTRSQNCNSPELKIKEMGSAVEVVLTCGLEDQFLFYEIIRIF 142
           L  L  S + S  +       S N NSPEL+IKEMGS VEVV   G EDQ LF E+I +F
Sbjct: 61  LASLNMSSAPSDRA-------STNNNSPELRIKEMGSIVEVVFKRGFEDQSLFDELIGVF 120

Query: 143 REERVEIINVSYSVLENTVLYSLHAEIEDVVYEFGAKKLTERLRRLVCESKSDEEMQAGA 202
            EER EIIN++YS+ E+ +LYS+HAEIEDVVYE GAKKL ERL +LV E KSD E+ AG 
Sbjct: 121 HEERAEIINLNYSIHEDMLLYSIHAEIEDVVYELGAKKLVERLNKLVYEWKSDAELLAGG 180

Query: 203 SSSSGHGGTDLPANTATTSSDRHF 227
           +SSSGHGGT  PA   T SSDRHF
Sbjct: 181 ASSSGHGGTHPPAKIPTMSSDRHF 197

[Close]

BLAST of Lag0009661 vs. TAIR 10
Match: AT4G20970.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 116.3 bits (290), Expect = 3.2e-26
Identity = 75/179 (41.90%), Postives = 116/179 (64.80%), Query Frame = 0


Query: 15  SIKVERKVMEKNRRTQMKLLYSKLNSLLPTHHSNELPLTVSDQIDEAIKYIKSLETKLEK 74
           S  V+RK +EKNRR QMK LYS+L SLLP HHS+  PLT+ DQ+DEA  YIK L+  +EK
Sbjct: 11  SRSVDRKTVEKNRRMQMKSLYSELISLLP-HHSSTEPLTLPDQLDEAANYIKKLQVNVEK 70

Query: 75  DKEKKESFL--RRLKRSLSTSSSSQHTVPTRSQNCNSPELKIKEMGSAVEVVLTCGLEDQ 134
            +E+K + +    L++  S  SSS  +    S     P+++I+E GS   + L   LE +
Sbjct: 71  KRERKRNLVATTTLEKLNSVGSSSVSSSVDVSVPRKLPKIEIQETGSIFHIFLVTSLEHK 130

Query: 135 FLFYEIIRIFREE-RVEIINVSYSVLENTVLYSLHAEIEDVVYEFGAK-KLTERLRRLV 190
           F+F EIIR+  EE   EI +  YS++++ V ++LH ++E+  +++GA+ ++ ERL ++V
Sbjct: 131 FMFCEIIRVLTEELGAEITHAGYSIVDDAVFHTLHCKVEE--HDYGARSQIPERLEKIV 186

[Close]

BLAST of Lag0009661 vs. TAIR 10
Match: AT5G51780.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 54.7 bits (130), Expect = 1.1e-07
Identity = 46/175 (26.29%), Postives = 92/175 (52.57%), Query Frame = 0


Query: 17  KVERKVMEKNRRTQMKLLYSKLNSLLPTHHSNELPLTVSDQIDEAIKYIKSLETKLEKDK 76
           K+  +  E+ RR +M  LY+ L SLLP H       T SDQ++EA+ YIK L+ K+++  
Sbjct: 3   KMMHRETERQRRQEMASLYASLRSLLPLHFIKGKRST-SDQVNEAVNYIKYLQRKIKELS 62

Query: 77  EKKESFLRRLKRSLSTSSSSQHTVPTRSQNCNSPELKIKEMGSAVEVVLT---CGLEDQF 136
            +++  +   + SL  SS+          +     + +++    VE++L+   CG   Q 
Sbjct: 63  VRRDDLMVLSRGSLLGSSNGDFKEDVEMIS-GKNHVVVRQCLVGVEIMLSSRCCG--GQP 122

Query: 137 LFYEIIRIFREERVEIINVSYSVLENTVLYSLHAEIEDVVYEFGAKKLTERLRRL 189
            F  ++++  E  + ++N   S++++ ++Y++ AE+ D+       +L +RL R+
Sbjct: 123 RFSSVLQVLSEYGLCLLNSISSIVDDRLVYTIQAEVNDMALMIDLAELEKRLIRM 173

[Close]

BLAST of Lag0009661 vs. TAIR 10
Match: AT1G10586.1 (basic helix-loop-helix (bHLH) DNA-binding superfamily protein )

HSP 1 Score: 50.1 bits (118), Expect = 2.8e-06
Identity = 48/191 (25.13%), Postives = 95/191 (49.74%), Query Frame = 0


Query: 1   MEGSREGNIQQNVGSIKVERKVMEKNRRTQMKLLYSKLNSLLPTHHSNELPLTVSDQIDE 60
           ME +RE   + +  S++ +R + EK RR +MK L+    S+L +H S    L V   ID+
Sbjct: 1   MERAREIG-EGSASSLREQRNLREKERRMRMKHLF----SILSSHVSPTRRLPVPQLIDQ 60

Query: 61  AIKYIKSLETKLEKDKEKKESFLRRLKRSLSTSSSSQHTVPTRSQNCN-SPELKIKEMGS 120
           A+ Y+  L+ K+        ++L  +KR +         V  RS+  +  P+L I+ + S
Sbjct: 61  AVSYMIQLKEKV--------NYLNEMKRRMLGGE-----VKNRSEGSSLLPKLSIRSLDS 120

Query: 121 AVEVVLTCGLEDQ-FLFYEIIRIFREERVEIINVSYSVLENTVLYSLHAEIEDVVYEFGA 180
            +E+ L   L  +  + ++++ +F EE  ++++ +   L +   Y++ A+          
Sbjct: 121 IIEMNLVMDLNMKGVMLHKLVSVFEEEGAQVMSANLQNLNDRTFYTIIAQAIICRIGIDP 173

Query: 181 KKLTERLRRLV 190
            ++ ERLR ++
Sbjct: 181 SRIEERLRDII 173

The following BLAST results are available for this feature:

BLAST of Lag0009661 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038902351.1	2.1e-67	68.56	transcription factor bHLH162-like [Benincasa hispida]	[more]
XP_038902350.1	1.2e-62	69.01	transcription factor bHLH162-like [Benincasa hispida]	[more]
KAG6604789.1	2.2e-61	65.50	Transcription factor basic helix-loop-helix 162, partial [Cucurbita argyrosperma...	[more]
XP_023512282.1	3.2e-60	69.90	transcription factor bHLH162-like [Cucurbita pepo subsp. pepo]	[more]
XP_022944108.1	7.8e-59	69.00	transcription factor bHLH162-like [Cucurbita moschata]	[more]

BLAST of Lag0009661 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JIJ7	4.5e-25	41.90	Transcription factor bHLH162 OS=Arabidopsis thaliana OX=3702 GN=BHLH162 PE=1 SV=...	[more]
Q9FLI1	1.6e-06	26.29	Transcription factor bHLH36 OS=Arabidopsis thaliana OX=3702 GN=BHLH36 PE=2 SV=1	[more]
Q9XIJ1	4.0e-05	25.13	Transcription factor bHLH168 OS=Arabidopsis thaliana OX=3702 GN=BHLH168 PE=3 SV=...	[more]

BLAST of Lag0009661 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FXP8	3.8e-59	69.00	transcription factor bHLH162-like OS=Cucurbita moschata OX=3662 GN=LOC111448656 ...	[more]
A0A6J1G8B1	2.1e-57	63.80	transcription factor bHLH162-like OS=Cucurbita moschata OX=3662 GN=LOC111451747 ...	[more]
A0A6J1G8Q1	1.0e-56	66.18	transcription factor bHLH162-like OS=Cucurbita moschata OX=3662 GN=LOC111451748 ...	[more]
A0A6J1JF05	3.0e-56	67.51	transcription factor bHLH162-like OS=Cucurbita maxima OX=3661 GN=LOC111483908 PE...	[more]
A0A6J1I4R7	6.7e-56	65.20	transcription factor bHLH162-like OS=Cucurbita maxima OX=3661 GN=LOC111469622 PE...	[more]

BLAST of Lag0009661 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G20970.1	3.2e-26	41.90	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT5G51780.1	1.1e-07	26.29	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]
AT1G10586.1	2.8e-06	25.13	basic helix-loop-helix (bHLH) DNA-binding superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 58..85
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 197..229
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 196..229
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 88..108
None	No IPR available	PANTHER	PTHR13935:SF63	HELIX LOOP HELIX DNA-BINDING DOMAIN PROTEIN	coord: 13..196
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	SMART	SM00353	finulus	coord: 21..74 e-value: 8.1E-5 score: 32.0
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	PFAM	PF00010	HLH	coord: 19..69 e-value: 9.1E-8 score: 32.0
IPR011598	Myc-type, basic helix-loop-helix (bHLH) domain	PROSITE	PS50888	BHLH	coord: 15..68 score: 12.086958
IPR036638	Helix-loop-helix DNA-binding domain superfamily	GENE3D	4.10.280.10		coord: 7..98 e-value: 2.2E-8 score: 35.9
IPR036638	Helix-loop-helix DNA-binding domain superfamily	SUPERFAMILY	47459	HLH, helix-loop-helix DNA-binding domain	coord: 16..90
IPR015660	Achaete-scute transcription factor-related	PANTHER	PTHR13935	ACHAETE-SCUTE TRANSCRIPTION FACTOR-RELATED	coord: 13..196

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0009661.1	Lag0009661.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006357	regulation of transcription by RNA polymerase II
cellular_component	GO:0005634	nucleus
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003700	DNA-binding transcription factor activity
molecular_function	GO:0046983	protein dimerization activity