Lag0009578 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0009578
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	indole-3-acetic acid-amido synthetase GH3.6-like
Location	chr9: 40508716 .. 40510815 (-)
RNA-Seq Expression	Lag0009578
Synteny	Lag0009578

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTTGCATGGTGTAGAAAACTTGATGGATGAGAAAGATAAACAAGCATTACAACATATTGAAGATGTCACAAGCAATGCCGAAGAAACCCAACAACAAATTCTAGCCGAAATTCTGTCAACAAATGCTCATGTTGAATACTTGCAACAACATGGTCTTAATGGGTCTACTGATGTTTCAACCTTCAAGAAGCTTATACCTATTATCTCTTATGAACAACTAAAACCTTATATAGAACGTATTGCCAACGGCGATGATTCTCCAATCCTATGTGCAGAGCCCATTACAGAGTTCTTTATAAGGTAACCTATTATTCTAAAGCACGATTTTTAAAGTTCTTATTGATATATTTACTATTATGTTTTTCTTACTTTTACATGTATGTGTGTGTGAGAAAGAGTATATATATGAATGTATATGAATAGGGGATCTTTTGCCTTTTATATATAGGTGTTTGTAAGATCTTTTTTGAGATGGGTTTTAAATAATGCCTAAGACAATGATTTTTTGCAGCACTGGGACATCAGGGGGAGAACACAAGTTAATTCCAATATTTGAACGAGAGCTTGTGAGAAGATTGTCTTTTTTTAACTATGTGATGCCAAGGATGAAAGAGCTATTTTCAAATAATTTTCACAAAGGCAAAGAAATGTACTTTCTTTTTGCAAGGGTTGAGTTAAGGACTAAAGCAGGAATTGTTGCTCGTGTAGCAAGTACAAGTTTCTGCAAAAGATCAAACTCCAATTATATACTAAGTGAAAACAATACGAGCCCTAAGGACATCATTCAGTGCACAGACTCATACCAAAGCCTATACAGTCAATTGCTCTGTGGGCTCTACCAGAACGAGATAGTTTTTCGAGTTGGTGCTCTTTTTGCCTCTACGTTAGCTCATGTTTTTAAATTCCTTGAAAATCATTGGGTTCTTTTGGCAAATGATATACGAACAGGAACTTTGAACCCCACAATTACAAACTCATCAGTGAGAGAATCAGTTATGAACATTCTGAAACCTAATCCTCAACTTGCAGATTTTATTGAAACAGAATGTAGTCGAGGAACATGGGAAGGGATCATTAAAAGATTGTGGCCCAACACCAAATTCATTGATGCTATTGTGACGGGAAGCATGTCACAGTACATTCCCCTACTAAATTATTACAGTAATGATCTCCCTCTTGTTTCTAACCACTATTGTTCTTCAGAATGCTTCTTTGGTTTAAACTTAGATCCATTATGTAAGCCTGAGGAAATCTCATACACTTTAATCCCCACCATGGCGTATTTCGAGTTCTTGCCAATTGATAGGACAAAAGCTAATGGAGAAGTAAGGCAAGAATTAGTTGATCTCATTGATGTCAAGTTAGGACAAGAATATGAGCTCGTAATCACTACTTATGCTGGCAAGACTCTCCTTCTTCCTAATATAATATATTTGATATTATCCTTTCAATTCTGGTTTATTTACATGAACATATTATTACAAAAAGATAAGCTAATCTTACTGTTGTTGCAGGGCTCTATCGTTATTGCATGGGTGACATTCTTCGCGTGACAGGATTTAAAAATAAAGCCCCAAAATTTAGTTTTGTATGCAGGAAAAGTGCTGGTCTAAGCATCGATGCTGAGAAAACGGATGAAGCTGAGCTTCAAAATGCCATACAAGAAGCTGCTCATATTTTAAGCCCATTTGGAGCGACTATTGTTGAGTACACTAGCTATGCAGATAGCTCAACAATTCCTGGACACTATGTGTTGTACATGGAAATTAGCTTTAAGAAAGAAGTCTCCATTCCATCTTCAATATATGAGGATTATTGTCTTGCTATTGAAGAATCGCTTAGTAGACTGTATCGCGTAGCAAGATCTCATGAAAAATGTATCAATCCATTGGAGATAAGGATTGTAGAGGTTGGAACTTTTGATAAACTCATGGAGCAAGCACTGAATCGGGGAGCTTCAATGAGTCAATACAAGACACCTCGATGTGTAAATTCGAAACAAATGATCGAGCTTTTGGACTCAAACGTTGCCTCCAATTATTTCAGTAAAAAATGCCCAAAACTGGATACCAATGGTCTGCACCGTCTTCTTTCCTGA

mRNA sequence

ATGTTGCATGGTGTAGAAAACTTGATGGATGAGAAAGATAAACAAGCATTACAACATATTGAAGATGTCACAAGCAATGCCGAAGAAACCCAACAACAAATTCTAGCCGAAATTCTGTCAACAAATGCTCATGTTGAATACTTGCAACAACATGGTCTTAATGGGTCTACTGATGTTTCAACCTTCAAGAAGCTTATACCTATTATCTCTTATGAACAACTAAAACCTTATATAGAACGTATTGCCAACGGCGATGATTCTCCAATCCTATGTGCAGAGCCCATTACAGAGTTCTTTATAAGCACTGGGACATCAGGGGGAGAACACAAGTTAATTCCAATATTTGAACGAGAGCTTGTGAGAAGATTGTCTTTTTTTAACTATGTGATGCCAAGGATGAAAGAGCTATTTTCAAATAATTTTCACAAAGGCAAAGAAATGTACTTTCTTTTTGCAAGGGTTGAGTTAAGGACTAAAGCAGGAATTGTTGCTCGTGTAGCAAGTACAAGTTTCTGCAAAAGATCAAACTCCAATTATATACTAAGTGAAAACAATACGAGCCCTAAGGACATCATTCAGTGCACAGACTCATACCAAAGCCTATACAGTCAATTGCTCTGTGGGCTCTACCAGAACGAGATAGTTTTTCGAGTTGGTGCTCTTTTTGCCTCTACGTTAGCTCATGTTTTTAAATTCCTTGAAAATCATTGGGTTCTTTTGGCAAATGATATACGAACAGGAACTTTGAACCCCACAATTACAAACTCATCAGTGAGAGAATCAGTTATGAACATTCTGAAACCTAATCCTCAACTTGCAGATTTTATTGAAACAGAATGTAGTCGAGGAACATGGGAAGGGATCATTAAAAGATTGTGGCCCAACACCAAATTCATTGATGCTATTGTGACGGGAAGCATGTCACAGTACATTCCCCTACTAAATTATTACAGTAATGATCTCCCTCTTGTTTCTAACCACTATTGTTCTTCAGAATGCTTCTTTGGTTTAAACTTAGATCCATTATGTAAGCCTGAGGAAATCTCATACACTTTAATCCCCACCATGGCGTATTTCGAGTTCTTGCCAATTGATAGGACAAAAGCTAATGGAGAAGTAAGGCAAGAATTAGTTGATCTCATTGATGTCAAGTTAGGACAAGAATATGAGCTCGTAATCACTACTTATGCTGGGCTCTATCGTTATTGCATGGGTGACATTCTTCGCGTGACAGGATTTAAAAATAAAGCCCCAAAATTTAGTTTTGTATGCAGGAAAAGTGCTGGTCTAAGCATCGATGCTGAGAAAACGGATGAAGCTGAGCTTCAAAATGCCATACAAGAAGCTGCTCATATTTTAAGCCCATTTGGAGCGACTATTGTTGAGTACACTAGCTATGCAGATAGCTCAACAATTCCTGGACACTATGTGTTGTACATGGAAATTAGCTTTAAGAAAGAAGTCTCCATTCCATCTTCAATATATGAGGATTATTGTCTTGCTATTGAAGAATCGCTTAGTAGACTGTATCGCGTAGCAAGATCTCATGAAAAATGTATCAATCCATTGGAGATAAGGATTGTAGAGGTTGGAACTTTTGATAAACTCATGGAGCAAGCACTGAATCGGGGAGCTTCAATGAGTCAATACAAGACACCTCGATGTGTAAATTCGAAACAAATGATCGAGCTTTTGGACTCAAACGTTGCCTCCAATTATTTCAGTAAAAAATGCCCAAAACTGGATACCAATGGTCTGCACCGTCTTCTTTCCTGA

Coding sequence (CDS)

ATGTTGCATGGTGTAGAAAACTTGATGGATGAGAAAGATAAACAAGCATTACAACATATTGAAGATGTCACAAGCAATGCCGAAGAAACCCAACAACAAATTCTAGCCGAAATTCTGTCAACAAATGCTCATGTTGAATACTTGCAACAACATGGTCTTAATGGGTCTACTGATGTTTCAACCTTCAAGAAGCTTATACCTATTATCTCTTATGAACAACTAAAACCTTATATAGAACGTATTGCCAACGGCGATGATTCTCCAATCCTATGTGCAGAGCCCATTACAGAGTTCTTTATAAGCACTGGGACATCAGGGGGAGAACACAAGTTAATTCCAATATTTGAACGAGAGCTTGTGAGAAGATTGTCTTTTTTTAACTATGTGATGCCAAGGATGAAAGAGCTATTTTCAAATAATTTTCACAAAGGCAAAGAAATGTACTTTCTTTTTGCAAGGGTTGAGTTAAGGACTAAAGCAGGAATTGTTGCTCGTGTAGCAAGTACAAGTTTCTGCAAAAGATCAAACTCCAATTATATACTAAGTGAAAACAATACGAGCCCTAAGGACATCATTCAGTGCACAGACTCATACCAAAGCCTATACAGTCAATTGCTCTGTGGGCTCTACCAGAACGAGATAGTTTTTCGAGTTGGTGCTCTTTTTGCCTCTACGTTAGCTCATGTTTTTAAATTCCTTGAAAATCATTGGGTTCTTTTGGCAAATGATATACGAACAGGAACTTTGAACCCCACAATTACAAACTCATCAGTGAGAGAATCAGTTATGAACATTCTGAAACCTAATCCTCAACTTGCAGATTTTATTGAAACAGAATGTAGTCGAGGAACATGGGAAGGGATCATTAAAAGATTGTGGCCCAACACCAAATTCATTGATGCTATTGTGACGGGAAGCATGTCACAGTACATTCCCCTACTAAATTATTACAGTAATGATCTCCCTCTTGTTTCTAACCACTATTGTTCTTCAGAATGCTTCTTTGGTTTAAACTTAGATCCATTATGTAAGCCTGAGGAAATCTCATACACTTTAATCCCCACCATGGCGTATTTCGAGTTCTTGCCAATTGATAGGACAAAAGCTAATGGAGAAGTAAGGCAAGAATTAGTTGATCTCATTGATGTCAAGTTAGGACAAGAATATGAGCTCGTAATCACTACTTATGCTGGGCTCTATCGTTATTGCATGGGTGACATTCTTCGCGTGACAGGATTTAAAAATAAAGCCCCAAAATTTAGTTTTGTATGCAGGAAAAGTGCTGGTCTAAGCATCGATGCTGAGAAAACGGATGAAGCTGAGCTTCAAAATGCCATACAAGAAGCTGCTCATATTTTAAGCCCATTTGGAGCGACTATTGTTGAGTACACTAGCTATGCAGATAGCTCAACAATTCCTGGACACTATGTGTTGTACATGGAAATTAGCTTTAAGAAAGAAGTCTCCATTCCATCTTCAATATATGAGGATTATTGTCTTGCTATTGAAGAATCGCTTAGTAGACTGTATCGCGTAGCAAGATCTCATGAAAAATGTATCAATCCATTGGAGATAAGGATTGTAGAGGTTGGAACTTTTGATAAACTCATGGAGCAAGCACTGAATCGGGGAGCTTCAATGAGTCAATACAAGACACCTCGATGTGTAAATTCGAAACAAATGATCGAGCTTTTGGACTCAAACGTTGCCTCCAATTATTTCAGTAAAAAATGCCCAAAACTGGATACCAATGGTCTGCACCGTCTTCTTTCCTGA

Protein sequence

MLHGVENLMDEKDKQALQHIEDVTSNAEETQQQILAEILSTNAHVEYLQQHGLNGSTDVSTFKKLIPIISYEQLKPYIERIANGDDSPILCAEPITEFFISTGTSGGEHKLIPIFERELVRRLSFFNYVMPRMKELFSNNFHKGKEMYFLFARVELRTKAGIVARVASTSFCKRSNSNYILSENNTSPKDIIQCTDSYQSLYSQLLCGLYQNEIVFRVGALFASTLAHVFKFLENHWVLLANDIRTGTLNPTITNSSVRESVMNILKPNPQLADFIETECSRGTWEGIIKRLWPNTKFIDAIVTGSMSQYIPLLNYYSNDLPLVSNHYCSSECFFGLNLDPLCKPEEISYTLIPTMAYFEFLPIDRTKANGEVRQELVDLIDVKLGQEYELVITTYAGLYRYCMGDILRVTGFKNKAPKFSFVCRKSAGLSIDAEKTDEAELQNAIQEAAHILSPFGATIVEYTSYADSSTIPGHYVLYMEISFKKEVSIPSSIYEDYCLAIEESLSRLYRVARSHEKCINPLEIRIVEVGTFDKLMEQALNRGASMSQYKTPRCVNSKQMIELLDSNVASNYFSKKCPKLDTNGLHRLLS

Homology

[Close]

BLAST of Lag0009578 vs. NCBI nr
Match: KAG6570863.1 (Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 897.9 bits (2319), Expect = 4.7e-257
Identity = 445/592 (75.17%), Postives = 506/592 (85.47%), Query Frame = 0


Query: 1   MLHGVENLMDEKDKQALQHIEDVTSNAEETQQQILAEILSTNAHVEYLQQHGLNGSTDVS 60
           M H  E   DE+DKQALQHIEDVT +A + Q+QIL +ILSTNA VEYLQQHGL+GST++S
Sbjct: 1   MAHVDEISSDERDKQALQHIEDVTIHASQIQRQILTQILSTNADVEYLQQHGLHGSTELS 60

Query: 61  TFKKLIPIISYEQLKPYIERIANGDDSPILCAEPITEFFISTGTSGGEHKLIPIFERELV 120
           TFK LIP+ISYEQL+PYI+RIANGDDSPILC  PITEFFIS+GTSGGE KLIP++E E +
Sbjct: 61  TFKNLIPLISYEQLRPYIDRIANGDDSPILCTNPITEFFISSGTSGGEPKLIPMYEEEFM 120

Query: 121 RRLSFFNYVMPRMKELFSNNF-HKGKEMYFLFARVELRTKAGIVARVASTSFCKRSNSNY 180
           RRLSFF+YVMPRMK+LF N+   KGK M+F FA+VEL+T++GIVA  A+TS CKR+N   
Sbjct: 121 RRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTATTSLCKRANL-- 180

Query: 181 ILSENNTSPKDIIQCTDSYQSLYSQLLCGLYQNEIVFRVGALFASTLAHVFKFLENHWVL 240
               NNTSPKDII CTD+ QSLY QLLCGL QN+IVFRVGALFASTL HV KFLE+HWV+
Sbjct: 181 ----NNTSPKDIILCTDTNQSLYCQLLCGLCQNDIVFRVGALFASTLIHVLKFLEDHWVV 240

Query: 241 LANDIRTGTLNPTITNSSVRESVMNILKPNPQLADFIETECSRGTWEGIIKRLWPNTKFI 300
           LA+DIR GTLN  IT+ SVRE+VMNIL PN QLADFIE ECS+GTWEGII RLWPN K+I
Sbjct: 241 LASDIRAGTLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGTWEGIITRLWPNAKYI 300

Query: 301 DAIVTGSMSQYIPLLNYYSNDLPLVSNHYCSSECFFGLNLDPLCKPEEISYTLIPTMAYF 360
           +AIVTGSM+QY+P LNYYSN LP+VS+HY  SECF GLNLDPLC P+  SYTLIPTMAYF
Sbjct: 301 NAIVTGSMAQYLPKLNYYSNHLPIVSDHYGGSECFLGLNLDPLCSPDRTSYTLIPTMAYF 360

Query: 361 EFLPIDRTKANGEVRQELVDLIDVKLGQEYELVITTYAGLYRYCMGDILRVTGFKNKAPK 420
           EFLPIDRTK++ E+ +ELVDL+DVKLG EYELVITTYAGLYRYCMGDILRVTGFKNKAP 
Sbjct: 361 EFLPIDRTKSSRELSRELVDLVDVKLGHEYELVITTYAGLYRYCMGDILRVTGFKNKAPN 420

Query: 421 FSFVCRKSAGLSIDAEKTDEAELQNAIQEAAHILSPFGATIVEYTSYADSSTIPGHYVLY 480
           FSFVCRK+  LS+D EKTDEAEL +A+QEAA +LSPFGA I +YTS+ADSS IPGHYVLY
Sbjct: 421 FSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIADYTSHADSSKIPGHYVLY 480

Query: 481 MEISFKKEVSIPSSIYEDYCLAIEESLSRLYRVARSHEKCINPLEIRIVEVGTFDKLMEQ 540
            EI  K  +SIPSSIYED C AIEE L+  YR  RSHEKCINPLEI+IV+VGTF+KLM Q
Sbjct: 481 WEIHVKGGISIPSSIYEDCCFAIEELLNGPYRYLRSHEKCINPLEIKIVKVGTFEKLMAQ 540

Query: 541 ALNRGASMSQYKTPRCVNSKQMIELLDSNVASNYFSKKCPKLDTNGLHRLLS 592
           ALNRGASMSQYKTPRCVNS  +IELLDSNV + YFSK+CPK+DTN LHRLLS
Sbjct: 541 ALNRGASMSQYKTPRCVNSTHIIELLDSNVVTKYFSKECPKVDTNALHRLLS 586

[Close]

BLAST of Lag0009578 vs. NCBI nr
Match: XP_022944596.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata])

HSP 1 Score: 889.0 bits (2296), Expect = 2.2e-254
Identity = 443/592 (74.83%), Postives = 503/592 (84.97%), Query Frame = 0


Query: 1   MLHGVENLMDEKDKQALQHIEDVTSNAEETQQQILAEILSTNAHVEYLQQHGLNGSTDVS 60
           M H  E   DE+DKQALQHIEDVT +A + Q+QIL +ILSTNA VEYLQQHGL+GST++S
Sbjct: 1   MAHVDEISSDERDKQALQHIEDVTIHASQIQRQILTQILSTNADVEYLQQHGLHGSTELS 60

Query: 61  TFKKLIPIISYEQLKPYIERIANGDDSPILCAEPITEFFISTGTSGGEHKLIPIFERELV 120
           TFK LIP+ISYEQL+PYI+RIANGDDSPILC  PITEFFIS+GTSGGE KLIP++E E +
Sbjct: 61  TFKNLIPLISYEQLRPYIDRIANGDDSPILCTNPITEFFISSGTSGGEPKLIPMYEEEFM 120

Query: 121 RRLSFFNYVMPRMKELFSNNF-HKGKEMYFLFARVELRTKAGIVARVASTSFCKRSNSNY 180
           RRLSFF+YVMPRMK+LF N+   KGK M+F FA+VEL+T++GIVA  A+TS CKR+N   
Sbjct: 121 RRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTATTSLCKRANL-- 180

Query: 181 ILSENNTSPKDIIQCTDSYQSLYSQLLCGLYQNEIVFRVGALFASTLAHVFKFLENHWVL 240
               NNTSPKDII CTD+ QSLY QLLCGL QN+IVFRVGALFASTL HV KFLE+HWV+
Sbjct: 181 ----NNTSPKDIILCTDTNQSLYCQLLCGLCQNDIVFRVGALFASTLIHVLKFLEDHWVV 240

Query: 241 LANDIRTGTLNPTITNSSVRESVMNILKPNPQLADFIETECSRGTWEGIIKRLWPNTKFI 300
           LA+DIR GTLN  IT+ SVRE+VMNIL PN QLADFIE ECS+GTWEGII RLWPN K+I
Sbjct: 241 LASDIRAGTLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGTWEGIITRLWPNAKYI 300

Query: 301 DAIVTGSMSQYIPLLNYYSNDLPLVSNHYCSSECFFGLNLDPLCKPEEISYTLIPTMAYF 360
           +AIVTGSM+QY+P LNYYSN LP+VS+HY +SECF GLNLDPLC P+  SYTLIPTMAYF
Sbjct: 301 NAIVTGSMAQYLPKLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDRTSYTLIPTMAYF 360

Query: 361 EFLPIDRTKANGEVRQELVDLIDVKLGQEYELVITTYAGLYRYCMGDILRVTGFKNKAPK 420
           EFLPIDRTK++ E+ +ELVDL+DVKLG EYELVITTYAGLYRY MGDIL VTGFKNKAP 
Sbjct: 361 EFLPIDRTKSSRELSRELVDLVDVKLGHEYELVITTYAGLYRYRMGDILGVTGFKNKAPN 420

Query: 421 FSFVCRKSAGLSIDAEKTDEAELQNAIQEAAHILSPFGATIVEYTSYADSSTIPGHYVLY 480
           FSFVCRK+  LS+D EKTDEAEL +A+QEAA +LSPFGA I +YTS+ADSS IPGHYVLY
Sbjct: 421 FSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIADYTSHADSSKIPGHYVLY 480

Query: 481 MEISFKKEVSIPSSIYEDYCLAIEESLSRLYRVARSHEKCINPLEIRIVEVGTFDKLMEQ 540
            EI  K  +SIPSSIYED C AIEE L+  YR  RSHEKCINPLEI+IV+VGTF+KLM Q
Sbjct: 481 WEIHVKGGISIPSSIYEDCCFAIEELLNGPYRYLRSHEKCINPLEIKIVKVGTFEKLMAQ 540

Query: 541 ALNRGASMSQYKTPRCVNSKQMIELLDSNVASNYFSKKCPKLDTNGLHRLLS 592
           ALNRGASMSQYKTPRCVNS  +IELLDSNV   YFSKKCPK+DT  LHRLLS
Sbjct: 541 ALNRGASMSQYKTPRCVNSTHIIELLDSNVVIKYFSKKCPKVDTKALHRLLS 586

[Close]

BLAST of Lag0009578 vs. NCBI nr
Match: XP_038902067.1 (indole-3-acetic acid-amido synthetase GH3.5-like [Benincasa hispida])

HSP 1 Score: 865.9 bits (2236), Expect = 2.0e-247
Identity = 434/595 (72.94%), Postives = 506/595 (85.04%), Query Frame = 0


Query: 1   MLHGVENLMDEKDKQALQHIEDVTSNAEETQQQILAEILSTNAHVEYLQQHGLNGSTDVS 60
           M H VE+ +DEKDK+ALQHIEDVTS A+E Q+QIL EILSTNA+VEYLQQHGL+GSTD S
Sbjct: 1   MSHVVEHWLDEKDKEALQHIEDVTSKADEIQRQILTEILSTNANVEYLQQHGLHGSTDAS 60

Query: 61  TFKKLIPIISYEQLKPYIERIANGDDSPILCAEPITEFFISTGTSGGEHKLIPIFERELV 120
           TFKKLIP+ISYEQL PY+  IA+ DDSPILC+ PIT FF S+GTSGGE KLIP++E E V
Sbjct: 61  TFKKLIPLISYEQLTPYLALIAHDDDSPILCSNPITHFFTSSGTSGGETKLIPMYEEEFV 120

Query: 121 RRLSFFNYVMPRMKELFSN-NFHKGKEMYFLFARVELRTKAGIVARVASTSFCKR-SNSN 180
           RRLSFFNY+MPRMK+LF N N+ KGK M F FA+ E +TKAGI+ R   TS  KR SN  
Sbjct: 121 RRLSFFNYMMPRMKQLFPNINWQKGKGMNFHFAKPEFKTKAGIIVRSIFTSLHKRSSNLK 180

Query: 181 YILSENNTSPKDIIQCTDSYQSLYSQLLCGLYQNEIVFRVGALFASTLAHVFKFLENHWV 240
                NNTSP DII CTDS+QSLY QLLCGLYQN++VFR+GALFASTL HVFKFLENHWV
Sbjct: 181 SKPIGNNTSPDDIILCTDSFQSLYCQLLCGLYQNDVVFRLGALFASTLIHVFKFLENHWV 240

Query: 241 LLANDIRTGTLNPTITNSSVRESVMNI-LKPNPQLADFIETECSRGTWEGIIKRLWPNTK 300
           +LANDI TG++NP ITN SVRESVM I +KPNP++AD IE ECS+G+WEGIIKRLWPNTK
Sbjct: 241 VLANDILTGSINPKITNLSVRESVMKIVVKPNPEVADLIEHECSKGSWEGIIKRLWPNTK 300

Query: 301 FIDAIVTGSMSQYIPLLNYYSNDLPLVSNHYCSSECFFGLNLDPLCKPEEISYTLIPTMA 360
           +I+AIVTGSMSQYIPLLN+Y+N+LP+VS+HY SSECF GLNLDPLC P EISYTLIPTMA
Sbjct: 301 YINAIVTGSMSQYIPLLNFYTNNLPIVSDHYGSSECFLGLNLDPLCDPYEISYTLIPTMA 360

Query: 361 YFEFLPIDRTKANGEVRQELVDLIDVKLGQEYELVITTYAGLYRYCMGDILRVTGFKNKA 420
           YFEFLPIDRT  NGEV Q+LVDL+DVKLGQEYELVITT+AGLYRY MGDI+RVTGF  KA
Sbjct: 361 YFEFLPIDRTNPNGEVTQQLVDLVDVKLGQEYELVITTFAGLYRYRMGDIIRVTGFTKKA 420

Query: 421 PKFSFVCRKSAGLSIDAEKTDEAELQNAIQEAAHILSPFGATIVEYTSYADSSTIPGHYV 480
           P FSFVCRK+  LS++ EKTDEA L  A++E  H+L PFGATIV+YTSYADSSTIPGHYV
Sbjct: 421 PNFSFVCRKNVVLSLNNEKTDEAGLHKAVEEGGHVLKPFGATIVDYTSYADSSTIPGHYV 480

Query: 481 LYMEISF----KKEV--SIPSSIYEDYCLAIEESLSRLYRVARSHEKCINPLEIRIVEVG 540
           LY E+ F    K ++  SIPSS+YED C AIEE+L+ LYR+ARSHEKCI+PLEI+IV+ G
Sbjct: 481 LYFELIFDEHNKAQISNSIPSSVYEDCCFAIEETLNSLYRLARSHEKCIDPLEIKIVKAG 540

Query: 541 TFDKLMEQALNRGASMSQYKTPRCVNSKQ--MIELLDSNVASNYFSKKCPKLDTN 585
           TF+KLM+ A+NRGAS+++YKTPRC+NSKQ  +I+LL+SN+ SNYFS+K PKLD N
Sbjct: 541 TFEKLMQLAINRGASITRYKTPRCLNSKQIHIIQLLESNLVSNYFSQKSPKLDAN 595

[Close]

BLAST of Lag0009578 vs. NCBI nr
Match: XP_022140778.1 (indole-3-acetic acid-amido synthetase GH3.6-like [Momordica charantia])

HSP 1 Score: 847.4 bits (2188), Expect = 7.3e-242
Identity = 420/581 (72.29%), Postives = 483/581 (83.13%), Query Frame = 0


Query: 9   MDEKDKQALQHIEDVTSNAEETQQQILAEILSTNAHVEYLQQHGLNGSTDVSTFKKLIPI 68
           +DEKDKQALQ +EDVT  A+E Q+++L EILSTNAHVEYLQQHGLNGSTD STFK LIPI
Sbjct: 4   LDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPI 63

Query: 69  ISYEQLKPYIERIANGDDSPILCAEPITEFFISTGTSGGEHKLIPIFERELVRRLSFFNY 128
           +SYE LKPYI+RI NGDDSPILCA PITEF+ STGTSGGE KLIP  E+E  R+LS  NY
Sbjct: 64  VSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNY 123

Query: 129 VMPRMKELFSNNFHKGKEMYFLFARVELRTKAGIVARVASTSFCKRSNSNYILSENN-TS 188
           + PRMK+LF    H GKEM+F FA+VE  TKAGIVAR  STSF K  + N +  E++ TS
Sbjct: 124 MRPRMKQLFP-GLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSYTS 183

Query: 189 PKDIIQCTDSYQSLYSQLLCGLYQNEIVFRVGALFASTLAHVFKFLENHWVLLANDIRTG 248
           P DI+ CTD YQSLY QLLCGL QN+ V RVG+LFASTL ++FKFLENHWVLLANDIRTG
Sbjct: 184 PTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRTG 243

Query: 249 TLNPTITNSSVRESVMNILKPNPQLADFIETECSRGTWEGIIKRLWPNTKFIDAIVTGSM 308
           T+N  I +S VR+SVMNILKP+PQLADFIE+ECS+GTWEGI+ RLWPNTKFI+A+VTGSM
Sbjct: 244 TINAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGSM 303

Query: 309 SQYIPLLNYYSNDLPLVSNHYCSSECFFGLNLDPLCKPEEISYTLIPTMAYFEFLPIDRT 368
           S YIP LNYY   LPL++  Y SSECFFG+NLDPLCKPEEISYT IPT+AYFEFLP+DRT
Sbjct: 304 SHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDRT 363

Query: 369 KANGEVRQELVDLIDVKLGQEYELVITTYAGLYRYCMGDILRVTGFKNKAPKFSFVCRKS 428
             +GEV QE VDL+DVKLGQEYE+V+TTYAGLYRYC+GDI+RVTGFKNKAP F FV RK+
Sbjct: 364 NDSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGRKN 423

Query: 429 AGLSIDAEKTDEAELQNAIQEAAHILSPFGATIVEYTSYADSSTIPGHYVLYMEISFKKE 488
             LS+D EKTDEAEL NA+ +   +LS FGA +V+YTSYADSST PGHYVLY EI FK+E
Sbjct: 424 VILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFKEE 483

Query: 489 VS-IPSSIYEDYCLAIEESLSRLYRVARSHEKCINPLEIRIVEVGTFDKLMEQALNRGAS 548
           +S IPSSIYED CLAIEESLS +YR ARSH+K INPLEI+IVE  TFDKLME+ +++GAS
Sbjct: 484 ISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQGAS 543

Query: 549 MSQYKTPRCVNSKQMIELLDSNVASNYFSKKCPKLDTNGLH 588
           +SQYKTPRCV S Q+IE LDS V +NYFSKK PK D    H
Sbjct: 544 ISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTRPH 583

[Close]

BLAST of Lag0009578 vs. NCBI nr
Match: XP_008448232.1 (PREDICTED: indole-3-acetic acid-amido synthetase GH3.6-like [Cucumis melo] >KAA0062188.1 indole-3-acetic acid-amido synthetase GH3.6-like [Cucumis melo var. makuwa] >TYK04840.1 indole-3-acetic acid-amido synthetase GH3.6-like [Cucumis melo var. makuwa])

HSP 1 Score: 817.0 bits (2109), Expect = 1.1e-232
Identity = 407/587 (69.34%), Postives = 485/587 (82.62%), Query Frame = 0


Query: 6   ENLMDEKDKQALQHIEDVTSNAEETQQQILAEILSTNAHVEYLQQHGLNGSTDVSTFKKL 65
           E  +DEKDK+ALQ+IED+T+ A+E Q+QIL EILS NA+VEYLQ+HGL+ STD STFKKL
Sbjct: 6   EKWLDEKDKEALQYIEDITTKADEIQRQILNEILSKNANVEYLQKHGLDASTDSSTFKKL 65

Query: 66  IPIISYEQLKPYIERIANGDDSPILCAEPITEFFISTGTSGGEHKLIPIFERELVRRLSF 125
           IP++ Y+QL+PYI RIA+GDDS ILC+ PITEFF S+GTSG EHKLIP++E+E VRRLSF
Sbjct: 66  IPLVYYQQLRPYIARIADGDDSSILCSNPITEFFKSSGTSGEEHKLIPMYEQEFVRRLSF 125

Query: 126 FNYVMPRMKELFSN-NFHKGKEMYFLFARVELRTKAGIVARVASTSFCKR-SNSNYILSE 185
           F+Y+ PRMK+LF N N+HK K + F FA+ E +TK GIV R   T+  KR SN   + S 
Sbjct: 126 FSYITPRMKQLFPNINWHKAKGLNFHFAKPEFKTKGGIVVRSIFTNLHKRSSNLRSMPSG 185

Query: 186 NNTSPKDIIQCTDSYQSLYSQLLCGLYQNEIVFRVGALFASTLAHVFKFLENHWVLLAND 245
           NNTSP DII CTDSYQSLY QLLCGLYQN++VFRV ALFASTL HVFKFLENHW+ LA D
Sbjct: 186 NNTSPDDIILCTDSYQSLYCQLLCGLYQNDVVFRVSALFASTLIHVFKFLENHWIDLATD 245

Query: 246 IRTGTLNPTITNSSVRESVMNI-LKPNPQLADFIETECSRGTWEGIIKRLWPNTKFIDAI 305
           IRT T+NP IT+SSVRESVM I +KPNP++AD IE EC +G WEGII RLWPN K+I+A+
Sbjct: 246 IRTRTINPKITSSSVRESVMKIVVKPNPEVADLIENECGKGRWEGIITRLWPNAKYINAV 305

Query: 306 VTGSMSQYIPLLNYYSNDLPLVSNHYCSSECFFGLNLDPLCKPEEISYTLIPTMAYFEFL 365
           VTGSMSQYIPLLNYY+N+LP+VS+HY SSECF GLNLDPLC P+E+SYTLIPTMAYFEFL
Sbjct: 306 VTGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCNPDEVSYTLIPTMAYFEFL 365

Query: 366 PIDRTKANGEVRQELVDLIDVKLGQEYELVITTYAGLYRYCMGDILRVTGFKNKAPKFSF 425
           PID     GEV Q LVDL+DVKLG EYELVITT+AGLYRYCMGD++RVTGFKNKAP FSF
Sbjct: 366 PIDMLNPKGEVNQRLVDLVDVKLGHEYELVITTFAGLYRYCMGDVVRVTGFKNKAPNFSF 425

Query: 426 VCRKSAGLSIDAEKTDEAELQNAIQEAAHILSPFGATIVEYTSYADSSTIPGHYVLYMEI 485
           V RK+  L+I  EKTDEA L  A++E   ++  FGATIV+YT+YAD STIPGHYVLY E+
Sbjct: 426 VRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKQFGATIVDYTTYADISTIPGHYVLYWEL 485

Query: 486 SFK--KEVS---IPSSIYEDYCLAIEESLSRLYRVARSHEKCINPLEIRIVEVGTFDKLM 545
           +    KE +   IP S++ED CLAIE SL+ LYR+ARSHEKCINPLEI+IV+ GTF+KLM
Sbjct: 486 TLDDLKEQNLNYIPCSVFEDCCLAIENSLNLLYRLARSHEKCINPLEIKIVKAGTFEKLM 545

Query: 546 EQALNRGASMSQYKTPRCVNSKQ--MIELLDSNVASNYFSKKCPKLD 583
           + ALNRGAS++QYKTPRC+NS Q  +I+LL+SN+ SNYFSK  P L+
Sbjct: 546 QLALNRGASITQYKTPRCLNSSQIHIIQLLESNLVSNYFSKNYPNLE 592

[Close]

BLAST of Lag0009578 vs. ExPASy Swiss-Prot
Match: O81829 (Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=GH3.5 PE=1 SV=1)

HSP 1 Score: 689.9 bits (1779), Expect = 2.6e-197
Identity = 343/586 (58.53%), Postives = 435/586 (74.23%), Query Frame = 0


Query: 9   MDEKDKQALQHIEDVTSNAEETQQQILAEILSTNAHVEYLQQHGLNGSTDVSTFKKLIPI 68
           +D+K+KQ LQ IE++TSNA++ Q+Q+L EIL+ NA VEYL++H LNG TD  TFK ++P+
Sbjct: 17  LDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFKNIMPV 76

Query: 69  ISYEQLKPYIERIANGDDSPILCAEPITEFFISTGTSGGEHKLIPIFERELVRRLSFFNY 128
           I+YE ++P I RIANGD SPIL ++PI+EF  S+GTSGGE KL+P  E EL RR   ++ 
Sbjct: 77  ITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSL 136

Query: 129 VMPRMKELFSNNFHKGKEMYFLFARVELRTKAGIVARVASTSFCKRSNSN---YILSENN 188
           +MP M + F      GK MYFLF + E +T  G+ AR   TS+ K S+     Y    N 
Sbjct: 137 LMPVMSQ-FVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNY 196

Query: 189 TSPKDIIQCTDSYQSLYSQLLCGLYQNEIVFRVGALFASTLAHVFKFLENHWVLLANDIR 248
           TSP + I C+DSYQS+YSQ+LCGL Q++ V RVGA+FAS      KFLE HW+ L  DIR
Sbjct: 197 TSPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIR 256

Query: 249 TGTLNPTITNSSVRESVMNILKPNPQLADFIETECSRGTWEGIIKRLWPNTKFIDAIVTG 308
           TGTL+  IT+ SVRE+V  ILKP+P+LADF+E EC + +W+GII RLWPNTK++D IVTG
Sbjct: 257 TGTLSSLITDPSVREAVAKILKPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTG 316

Query: 309 SMSQYIPLLNYYSNDLPLVSNHYCSSECFFGLNLDPLCKPEEISYTLIPTMAYFEFLPID 368
           +MSQYIP L+YYSN LPLV   Y SSEC+FG+NL PLCKP E+SYTLIP+MAYFEFLP+ 
Sbjct: 317 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPVH 376

Query: 369 RTKA-----------NGEVRQELVDLIDVKLGQEYELVITTYAGLYRYCMGDILRVTGFK 428
           R                + +QELVDL+DVKLGQEYELV+TTYAGL RY +GD+LRVTGFK
Sbjct: 377 RNNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVTGFK 436

Query: 429 NKAPKFSFVCRKSAGLSIDAEKTDEAELQNAIQEAAHILSPFGATIVEYTSYADSSTIPG 488
           NKAP+FSF+CRK+  LSID++KTDE ELQNA++ A   L PF A++ EYTSYAD+S+IPG
Sbjct: 437 NKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPG 496

Query: 489 HYVLYMEISFKKEVSIPSSIYEDYCLAIEESLSRLYRVARSHEKCINPLEIRIVEVGTFD 548
           HYVL+ E+       IP S++ED CLA+EES + +YR  R  +K I PLEI+IVE GTFD
Sbjct: 497 HYVLFWELCLDGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEIKIVEPGTFD 556

Query: 549 KLMEQALNRGASMSQYKTPRCVNSKQMIELLDSNVASNYFSKKCPK 581
           KLM+ A++ GAS++QYKTPRCV    +IELL+S V  +YFS KCPK
Sbjct: 557 KLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPK 601

[Close]

BLAST of Lag0009578 vs. ExPASy Swiss-Prot
Match: Q9LSQ4 (Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=GH3.6 PE=1 SV=1)

HSP 1 Score: 686.8 bits (1771), Expect = 2.2e-196
Identity = 343/584 (58.73%), Postives = 430/584 (73.63%), Query Frame = 0


Query: 11  EKDKQALQHIEDVTSNAEETQQQILAEILSTNAHVEYLQQHGLNGSTDVSTFKKLIPIIS 70
           EK+K  LQ IEDVT+NA++ Q+++L EILS NA VEYL++HGL G TD  TFK ++P+++
Sbjct: 19  EKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPVVT 78

Query: 71  YEQLKPYIERIANGDDSPILCAEPITEFFISTGTSGGEHKLIPIFERELVRRLSFFNYVM 130
           YE ++P I RIANGD S +LC+ PI+EF  S+GTSGGE KL+P  E EL RR   ++ +M
Sbjct: 79  YEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLM 138

Query: 131 PRMKELFSNNFHKGKEMYFLFARVELRTKAGIVARVASTSFCKRS---NSNYILSENNTS 190
           P M + F     KGK MYFLF + E +T  G+ AR   TS+ K S   N  Y    N TS
Sbjct: 139 PVMDQ-FVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYTS 198

Query: 191 PKDIIQCTDSYQSLYSQLLCGLYQNEIVFRVGALFASTLAHVFKFLENHWVLLANDIRTG 250
           P   I C+DSYQS+YSQ+LCGL Q++ V RVGA+FAS      KFLE HW  LA DIRTG
Sbjct: 199 PNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRTG 258

Query: 251 TLNPTITNSSVRESVMNILKPNPQLADFIETECSRGTWEGIIKRLWPNTKFIDAIVTGSM 310
           TL+  IT+SSVRE+V  ILKP+P+LADF+E+EC + +W+GII RLWPNTK++D IVTG+M
Sbjct: 259 TLSSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGTM 318

Query: 311 SQYIPLLNYYSNDLPLVSNHYCSSECFFGLNLDPLCKPEEISYTLIPTMAYFEFLPIDRT 370
           SQYIP L+YYSN LPLV   Y SSEC+FG+NL PLCKP E+SYTLIP MAYFEFLP+ R 
Sbjct: 319 SQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHRN 378

Query: 371 KA-----------NGEVRQELVDLIDVKLGQEYELVITTYAGLYRYCMGDILRVTGFKNK 430
                          + +QELVDL+DVKLGQEYELV+TTYAGLYRY +GD+L V GFKN 
Sbjct: 379 SGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFKNN 438

Query: 431 APKFSFVCRKSAGLSIDAEKTDEAELQNAIQEAAHILSPFGATIVEYTSYADSSTIPGHY 490
           AP+FSF+CRK+  LSID++KTDE ELQNA++ A   L PF A++ EYTSYAD+S+IPGHY
Sbjct: 439 APQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHY 498

Query: 491 VLYMEISFKKEVSIPSSIYEDYCLAIEESLSRLYRVARSHEKCINPLEIRIVEVGTFDKL 550
           VL+ E+       IP S++ED CL IEESL+ +YR  R  +K I PLEI++VE GTFDKL
Sbjct: 499 VLFWELCLNGNTPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESGTFDKL 558

Query: 551 MEQALNRGASMSQYKTPRCVNSKQMIELLDSNVASNYFSKKCPK 581
           M+ A++ GAS++QYKTPRCV    +IELL+S V  +YFS KCPK
Sbjct: 559 MDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPK 601

[Close]

BLAST of Lag0009578 vs. ExPASy Swiss-Prot
Match: Q8LQM5 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 622.9 bits (1605), Expect = 3.8e-177
Identity = 309/574 (53.83%), Postives = 410/574 (71.43%), Query Frame = 0


Query: 15  QALQHIEDVTSNAEETQQQILAEILSTNAHVEYLQQHGLNGSTD-VSTFKKLIPIISYEQ 74
           +AL+ IE VT+NA + Q+++L EIL+ NA  EYL+++G+ GS D V  F++L+P+++YE 
Sbjct: 22  EALEFIEHVTANAGQVQRRVLGEILAQNAPAEYLRRYGIPGSPDVVDAFRRLVPLVTYEG 81

Query: 75  LKPYIERIANGDDSPILCAEPITEFFISTGTSGGEHKLIPIFERELVRRLSFFNYVMPRM 134
           L+P I RIANGD SPI   +PI+EF  S+GTSGGE KL+P    E+ RR   ++ +MP M
Sbjct: 82  LQPDILRIANGDTSPIFSGKPISEFLTSSGTSGGERKLMPTIADEMNRRSLLYSLLMPVM 141

Query: 135 KELFSNNFHKGKEMYFLFARVELRTKAGIVARVASTS------FCKRSNSNYILSENNTS 194
            +  S    KGK MY LF + E RT  G+ AR   TS      F  R    Y    + TS
Sbjct: 142 SQSVS-GLDKGKAMYLLFVKAESRTPGGLAARPVLTSYYRSRQFLDRPRDPY---TSYTS 201

Query: 195 PKDIIQCTDSYQSLYSQLLCGLYQNEIVFRVGALFASTLAHVFKFLENHWVLLANDIRTG 254
           P + I C DSYQS+Y+QLLCGL     V RVGA+FAS       FLE HW  L +DIRTG
Sbjct: 202 PDEAILCVDSYQSMYAQLLCGLVHRADVLRVGAVFASGFLRAIHFLEKHWARLCHDIRTG 261

Query: 255 TLNPTITNSSVRESVMNILKPNPQLADFIETECSRGTWEGIIKRLWPNTKFIDAIVTGSM 314
            L+P IT+  VR++V  +L+ +P LAD IE EC+R +WEGII+RLWP TK+ID IVTG+M
Sbjct: 262 ELDPEITDRVVRDAVGRVLRADPALADAIEDECARASWEGIIRRLWPRTKYIDVIVTGTM 321

Query: 315 SQYIPLLNYYSNDLPLVSNHYCSSECFFGLNLDPLCKPEEISYTLIPTMAYFEFLPIDRT 374
           SQYIP L +Y   LPL    Y SSEC+FGLNL+P+CKP +++YTLIPTM Y+EFLP++  
Sbjct: 322 SQYIPTLEFYGGGLPLTCTMYASSECYFGLNLNPMCKPSDVAYTLIPTMCYYEFLPVNCN 381

Query: 375 KANGEV-RQELVDLIDVKLGQEYELVITTYAGLYRYCMGDILRVTGFKNKAPKFSFVCRK 434
            A  E   ++LVDL+DVKLG EYELV+TTY+GLYRY +GD+LRV GFKNKAP FSFV R+
Sbjct: 382 NATAEASHRDLVDLVDVKLGHEYELVVTTYSGLYRYRVGDVLRVAGFKNKAPMFSFVRRQ 441

Query: 435 SAGLSIDAEKTDEAELQNAIQEAAHILSPFGATIVEYTSYADSSTIPGHYVLYMEISFKK 494
           +  LS+D++KTDE EL  A+  A   L+PFGA++VEYTSYAD++TIPGHYVL+ E+    
Sbjct: 442 NVALSVDSDKTDETELHAAVSGAVQHLAPFGASLVEYTSYADAATIPGHYVLFWELR-AG 501

Query: 495 EVSIPSSIYEDYCLAIEESLSRLYRVARSHEKCINPLEIRIVEVGTFDKLMEQALNRGAS 554
             ++P+S++E+ CL++EE+L+ +YR  R+ ++ I PLEIR+V  GTFDKLM+ A++RGAS
Sbjct: 502 STAVPASVFEECCLSVEEALNSVYRQGRACDRSIGPLEIRVVAEGTFDKLMDYAISRGAS 561

Query: 555 MSQYKTPRCVNSKQMIELLDSNVASNYFSKKCPK 581
           ++QYK PRCV    ++ELLD+ V   YFS KCPK
Sbjct: 562 INQYKAPRCVRPGPVVELLDARVQGKYFSPKCPK 590

[Close]

BLAST of Lag0009578 vs. ExPASy Swiss-Prot
Match: Q60EJ6 (Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japonica OX=39947 GN=GH3.4 PE=2 SV=1)

HSP 1 Score: 594.0 bits (1530), Expect = 1.9e-168
Identity = 307/599 (51.25%), Postives = 411/599 (68.61%), Query Frame = 0


Query: 9   MDEKDKQALQHIEDVTSNAEETQQQILAEILSTNAHVEYLQQHGLNGSTDVS--TFKKLI 68
           M  + ++ L+HIE VT NA + Q+++L EIL+ NA  EYL++ G+ G    +   F++L 
Sbjct: 19  MCAEHREKLEHIERVTRNAGQEQRRVLEEILAQNAQAEYLRRLGVPGDAPGADEAFRRLA 78

Query: 69  PIISYEQLKPYIERIANGDDSPILCAEPITEFFISTGTSGGEHKLIPIFERELVRRLSFF 128
           P+++YE + P + RIANGD SPIL  +P++EF  S+GTSGGE KL+P  E E+ RR   +
Sbjct: 79  PLVTYEDILPDVLRIANGDTSPILSGKPVSEFLTSSGTSGGERKLMPTIEEEMERRSGLY 138

Query: 129 NYVMPRMKELFSNNFHKGKEMYFLFARVELRTKAGIVARVASTSFCKRSNSNYILSENN- 188
           + +MP M         KGK MY  F + E RT  G+ AR   TSF +   S Y L   + 
Sbjct: 139 SLLMPVMSRQVP-GLDKGKAMYLYFVKSEWRTPGGLPARPVLTSFYR---SRYFLERPHD 198

Query: 189 -----TSPKDIIQCTDSYQSLYSQLLCGLYQNEIVFRVGALFASTLAHVFKFLENHWVLL 248
                TSP + + C D+YQS+Y+QL+CGL     V RVGA+FAS      +FLE HW  L
Sbjct: 199 PYTVYTSPDEAVLCEDAYQSMYAQLICGLVHRADVLRVGAVFASGFLRAIRFLEKHWPSL 258

Query: 249 ANDIRTGTLNPTITNSSVRESVMNILK-PNPQLADFIETECSRGTWEGIIKRLWPNTKFI 308
             DIR G L+  +T+ +VR +V  +L+  +P LAD IE EC+R +W+GII+R+WP+TK+I
Sbjct: 259 CRDIRAGELDGGVTDPAVRGAVGRVLRGADPALADAIEAECARPSWQGIIRRVWPSTKYI 318

Query: 309 DAIVTGSMSQYIPLLNYYSNDLPLVSNHYCSSECFFGLNLDPLCKPEEISYTLIPTMAYF 368
           D IVTG+M+QYIP L +Y   LPL    Y SSEC+FGLNL+P+CKP E++YTLIPTM YF
Sbjct: 319 DVIVTGAMAQYIPTLEFYGGGLPLACTMYASSECYFGLNLNPMCKPSEVAYTLIPTMCYF 378

Query: 369 EFLPIDRTKANGEVRQE-----LVDLIDVKLGQEYELVITTYAGLYRYCMGDILRVTGFK 428
           EFLP++ + AN     E     LVDL+DVKLG EYELV+TTY+GLYRY +GD+LRV GFK
Sbjct: 379 EFLPVN-SGANDVAAPEPDHRGLVDLVDVKLGHEYELVVTTYSGLYRYRVGDVLRVAGFK 438

Query: 429 NKAPKFSFVCRKSAGLSIDAEKTDEAELQNAIQEAAHILSPFGATIVEYTSYAD-SSTIP 488
           N AP F+FV RK+  LSID++KTDEAEL  A+ EA   L+PFGA++VEYTSYAD ++TIP
Sbjct: 439 NAAPMFAFVRRKNVALSIDSDKTDEAELHAAVTEAVQHLAPFGASLVEYTSYADTATTIP 498

Query: 489 GHYVLYMEI-SFKKEVSIPSSIYEDYCLAIEESLSRLYRVARSHEKCINPLEIRIVEVGT 548
           GHYVL+ E+ S      +P+S++ED CLA+EE L+ +YR  R+ ++ I PLEIR+V  GT
Sbjct: 499 GHYVLFWELRSPAGGTPVPASVFEDCCLAVEEGLNSVYRQCRAADRSIGPLEIRVVADGT 558

Query: 549 FDKLMEQALNRGASMSQYKTPRCVNSKQMIELLDSNVASNYFSKKCPKLDTNGLHRLLS 592
           FDKLM+ AL+RGAS++QYK PRCV    ++ELLD  V + YFS KCPK    G  + +S
Sbjct: 559 FDKLMDYALSRGASINQYKAPRCVRPGPVVELLDGRVQATYFSPKCPKWCAGGNKQWIS 612

[Close]

BLAST of Lag0009578 vs. ExPASy Swiss-Prot
Match: O82333 (Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=3702 GN=GH3.1 PE=2 SV=1)

HSP 1 Score: 580.9 bits (1496), Expect = 1.7e-164
Identity = 300/574 (52.26%), Postives = 395/574 (68.82%), Query Frame = 0


Query: 11  EKDKQALQHIEDVTSNAEETQQQILAEILSTNAHVEYLQQHGLNGSTDVSTFKKLIPIIS 70
           EKD +AL+ IE++T NA+  Q+ +LAEIL+ NA  EYL++  L G+TD  TFK  IP+I+
Sbjct: 17  EKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTFKTKIPVIT 76

Query: 71  YEQLKPYIERIANGDDSPILCAEPITEFFISTGTSGGEHKLIPIFERELVRRLSFFNYVM 130
           YE L+P I+RIA+GD SPIL A PI+EF  S+GTS GE KL+P  + EL RR   ++ +M
Sbjct: 77  YEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLM 136

Query: 131 PRMKELFSNNFHKGKEMYFLFARVELRTKAGIVARVASTSFCKRSNSN---YILSENNTS 190
           P M  L+     KGK MYFLF + E +T  G+ AR   TS+ K  +     Y      TS
Sbjct: 137 PVM-NLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVYTS 196

Query: 191 PKDIIQCTDSYQSLYSQLLCGLYQNEIVFRVGALFASTLAHVFKFLENHWVLLANDIRTG 250
           P + I C DS+QS+Y+Q+LCGL     V RVGA+FAS L    +FL+ HW   A+DI  G
Sbjct: 197 PNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIELG 256

Query: 251 TLNPTITNSSVRESVMNILKPNPQLADFIETECSRGTWEGIIKRLWPNTKFIDAIVTGSM 310
            L+  IT+ S+R+ +  ILKP+P LA+FI  EC    WE II R+WPNTK++D IVTG+M
Sbjct: 257 CLDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVTGAM 316

Query: 311 SQYIPLLNYYSNDLPLVSNHYCSSECFFGLNLDPLCKPEEISYTLIPTMAYFEFLPIDRT 370
           +QYIP L YYS  LP+    Y SSEC+FGLNL+P+ KP E+SYT++P MAYFEF+P+  T
Sbjct: 317 AQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIPLGGT 376

Query: 371 KANGEVRQELVDLIDVKLGQEYELVITTYAGLYRYCMGDILRVTGFKNKAPKFSFVCRKS 430
           KA        V+L+DV +G+EYELV+TTYAGL RY +GDILRVTGF N AP+F FV RK+
Sbjct: 377 KA--------VELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAPQFHFVRRKN 436

Query: 431 AGLSIDAEKTDEAELQNAIQEAAHIL-SPFGATIVEYTSYADSSTIPGHYVLYMEISFKK 490
             LSID++KTDE+ELQ A++ A+ IL    G+ + EYTSYAD+STIPGHYVLY E+  + 
Sbjct: 437 VLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTIPGHYVLYWELLVRD 496

Query: 491 EVSIPS-SIYEDYCLAIEESLSRLYRVARSHEKCINPLEIRIVEVGTFDKLMEQALNRGA 550
               PS       CL +EESL+ +YR +R  +  + PLEIR+V  GTF++LM+ A++RGA
Sbjct: 497 GARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNGTFEELMDYAISRGA 556

Query: 551 SMSQYKTPRCVNSKQMIELLDSNVASNYFSKKCP 580
           S++QYK PRCVN   ++ELLDS V S +FS   P
Sbjct: 557 SINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLP 581

[Close]

BLAST of Lag0009578 vs. ExPASy TrEMBL
Match: A0A6J1FUU5 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 GN=LOC111449009 PE=3 SV=1)

HSP 1 Score: 889.0 bits (2296), Expect = 1.1e-254
Identity = 443/592 (74.83%), Postives = 503/592 (84.97%), Query Frame = 0


Query: 1   MLHGVENLMDEKDKQALQHIEDVTSNAEETQQQILAEILSTNAHVEYLQQHGLNGSTDVS 60
           M H  E   DE+DKQALQHIEDVT +A + Q+QIL +ILSTNA VEYLQQHGL+GST++S
Sbjct: 1   MAHVDEISSDERDKQALQHIEDVTIHASQIQRQILTQILSTNADVEYLQQHGLHGSTELS 60

Query: 61  TFKKLIPIISYEQLKPYIERIANGDDSPILCAEPITEFFISTGTSGGEHKLIPIFERELV 120
           TFK LIP+ISYEQL+PYI+RIANGDDSPILC  PITEFFIS+GTSGGE KLIP++E E +
Sbjct: 61  TFKNLIPLISYEQLRPYIDRIANGDDSPILCTNPITEFFISSGTSGGEPKLIPMYEEEFM 120

Query: 121 RRLSFFNYVMPRMKELFSNNF-HKGKEMYFLFARVELRTKAGIVARVASTSFCKRSNSNY 180
           RRLSFF+YVMPRMK+LF N+   KGK M+F FA+VEL+T++GIVA  A+TS CKR+N   
Sbjct: 121 RRLSFFDYVMPRMKQLFPNSLVGKGKGMHFQFAKVELKTRSGIVAHTATTSLCKRANL-- 180

Query: 181 ILSENNTSPKDIIQCTDSYQSLYSQLLCGLYQNEIVFRVGALFASTLAHVFKFLENHWVL 240
               NNTSPKDII CTD+ QSLY QLLCGL QN+IVFRVGALFASTL HV KFLE+HWV+
Sbjct: 181 ----NNTSPKDIILCTDTNQSLYCQLLCGLCQNDIVFRVGALFASTLIHVLKFLEDHWVV 240

Query: 241 LANDIRTGTLNPTITNSSVRESVMNILKPNPQLADFIETECSRGTWEGIIKRLWPNTKFI 300
           LA+DIR GTLN  IT+ SVRE+VMNIL PN QLADFIE ECS+GTWEGII RLWPN K+I
Sbjct: 241 LASDIRAGTLNTKITDPSVREAVMNILNPNAQLADFIEAECSKGTWEGIITRLWPNAKYI 300

Query: 301 DAIVTGSMSQYIPLLNYYSNDLPLVSNHYCSSECFFGLNLDPLCKPEEISYTLIPTMAYF 360
           +AIVTGSM+QY+P LNYYSN LP+VS+HY +SECF GLNLDPLC P+  SYTLIPTMAYF
Sbjct: 301 NAIVTGSMAQYLPKLNYYSNHLPIVSDHYGASECFLGLNLDPLCSPDRTSYTLIPTMAYF 360

Query: 361 EFLPIDRTKANGEVRQELVDLIDVKLGQEYELVITTYAGLYRYCMGDILRVTGFKNKAPK 420
           EFLPIDRTK++ E+ +ELVDL+DVKLG EYELVITTYAGLYRY MGDIL VTGFKNKAP 
Sbjct: 361 EFLPIDRTKSSRELSRELVDLVDVKLGHEYELVITTYAGLYRYRMGDILGVTGFKNKAPN 420

Query: 421 FSFVCRKSAGLSIDAEKTDEAELQNAIQEAAHILSPFGATIVEYTSYADSSTIPGHYVLY 480
           FSFVCRK+  LS+D EKTDEAEL +A+QEAA +LSPFGA I +YTS+ADSS IPGHYVLY
Sbjct: 421 FSFVCRKNVVLSLDIEKTDEAELHDAVQEAARLLSPFGAAIADYTSHADSSKIPGHYVLY 480

Query: 481 MEISFKKEVSIPSSIYEDYCLAIEESLSRLYRVARSHEKCINPLEIRIVEVGTFDKLMEQ 540
            EI  K  +SIPSSIYED C AIEE L+  YR  RSHEKCINPLEI+IV+VGTF+KLM Q
Sbjct: 481 WEIHVKGGISIPSSIYEDCCFAIEELLNGPYRYLRSHEKCINPLEIKIVKVGTFEKLMAQ 540

Query: 541 ALNRGASMSQYKTPRCVNSKQMIELLDSNVASNYFSKKCPKLDTNGLHRLLS 592
           ALNRGASMSQYKTPRCVNS  +IELLDSNV   YFSKKCPK+DT  LHRLLS
Sbjct: 541 ALNRGASMSQYKTPRCVNSTHIIELLDSNVVIKYFSKKCPKVDTKALHRLLS 586

[Close]

BLAST of Lag0009578 vs. ExPASy TrEMBL
Match: A0A6J1CIS9 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 GN=LOC111011358 PE=3 SV=1)

HSP 1 Score: 847.4 bits (2188), Expect = 3.5e-242
Identity = 420/581 (72.29%), Postives = 483/581 (83.13%), Query Frame = 0


Query: 9   MDEKDKQALQHIEDVTSNAEETQQQILAEILSTNAHVEYLQQHGLNGSTDVSTFKKLIPI 68
           +DEKDKQALQ +EDVT  A+E Q+++L EILSTNAHVEYLQQHGLNGSTD STFK LIPI
Sbjct: 4   LDEKDKQALQDLEDVTRTADEIQRRVLTEILSTNAHVEYLQQHGLNGSTDASTFKNLIPI 63

Query: 69  ISYEQLKPYIERIANGDDSPILCAEPITEFFISTGTSGGEHKLIPIFERELVRRLSFFNY 128
           +SYE LKPYI+RI NGDDSPILCA PITEF+ STGTSGGE KLIP  E+E  R+LS  NY
Sbjct: 64  VSYEHLKPYIDRIVNGDDSPILCAHPITEFYTSTGTSGGEQKLIPTIEQEFARKLSISNY 123

Query: 129 VMPRMKELFSNNFHKGKEMYFLFARVELRTKAGIVARVASTSFCKRSNSNYILSENN-TS 188
           + PRMK+LF    H GKEM+F FA+VE  TKAGIVAR  STSF K  + N +  E++ TS
Sbjct: 124 MRPRMKQLFP-GLHNGKEMFFNFAKVESITKAGIVARSVSTSFLKSPHLNNVKVESSYTS 183

Query: 189 PKDIIQCTDSYQSLYSQLLCGLYQNEIVFRVGALFASTLAHVFKFLENHWVLLANDIRTG 248
           P DI+ CTD YQSLY QLLCGL QN+ V RVG+LFASTL ++FKFLENHWVLLANDIRTG
Sbjct: 184 PTDILCCTDVYQSLYCQLLCGLCQNQKVVRVGSLFASTLIYIFKFLENHWVLLANDIRTG 243

Query: 249 TLNPTITNSSVRESVMNILKPNPQLADFIETECSRGTWEGIIKRLWPNTKFIDAIVTGSM 308
           T+N  I +S VR+SVMNILKP+PQLADFIE+ECS+GTWEGI+ RLWPNTKFI+A+VTGSM
Sbjct: 244 TINAKIMDSYVRKSVMNILKPDPQLADFIESECSKGTWEGIVTRLWPNTKFINAVVTGSM 303

Query: 309 SQYIPLLNYYSNDLPLVSNHYCSSECFFGLNLDPLCKPEEISYTLIPTMAYFEFLPIDRT 368
           S YIP LNYY   LPL++  Y SSECFFG+NLDPLCKPEEISYT IPT+AYFEFLP+DRT
Sbjct: 304 SHYIPQLNYYCKSLPLITVFYASSECFFGINLDPLCKPEEISYTFIPTVAYFEFLPVDRT 363

Query: 369 KANGEVRQELVDLIDVKLGQEYELVITTYAGLYRYCMGDILRVTGFKNKAPKFSFVCRKS 428
             +GEV QE VDL+DVKLGQEYE+V+TTYAGLYRYC+GDI+RVTGFKNKAP F FV RK+
Sbjct: 364 NDSGEVGQEFVDLVDVKLGQEYEIVVTTYAGLYRYCIGDIVRVTGFKNKAPNFRFVGRKN 423

Query: 429 AGLSIDAEKTDEAELQNAIQEAAHILSPFGATIVEYTSYADSSTIPGHYVLYMEISFKKE 488
             LS+D EKTDEAEL NA+ +   +LS FGA +V+YTSYADSST PGHYVLY EI FK+E
Sbjct: 424 VILSLDVEKTDEAELHNAVNKVGSLLSSFGAILVDYTSYADSSTFPGHYVLYWEIQFKEE 483

Query: 489 VS-IPSSIYEDYCLAIEESLSRLYRVARSHEKCINPLEIRIVEVGTFDKLMEQALNRGAS 548
           +S IPSSIYED CLAIEESLS +YR ARSH+K INPLEI+IVE  TFDKLME+ +++GAS
Sbjct: 484 ISIIPSSIYEDCCLAIEESLSSVYRRARSHQKVINPLEIKIVEARTFDKLMERMVDQGAS 543

Query: 549 MSQYKTPRCVNSKQMIELLDSNVASNYFSKKCPKLDTNGLH 588
           +SQYKTPRCV S Q+IE LDS V +NYFSKK PK D    H
Sbjct: 544 ISQYKTPRCVKSTQIIEFLDSKVVANYFSKKYPKWDPTRPH 583

[Close]

BLAST of Lag0009578 vs. ExPASy TrEMBL
Match: A0A5A7V3L5 (Indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold143G00190 PE=3 SV=1)

HSP 1 Score: 817.0 bits (2109), Expect = 5.1e-233
Identity = 407/587 (69.34%), Postives = 485/587 (82.62%), Query Frame = 0


Query: 6   ENLMDEKDKQALQHIEDVTSNAEETQQQILAEILSTNAHVEYLQQHGLNGSTDVSTFKKL 65
           E  +DEKDK+ALQ+IED+T+ A+E Q+QIL EILS NA+VEYLQ+HGL+ STD STFKKL
Sbjct: 6   EKWLDEKDKEALQYIEDITTKADEIQRQILNEILSKNANVEYLQKHGLDASTDSSTFKKL 65

Query: 66  IPIISYEQLKPYIERIANGDDSPILCAEPITEFFISTGTSGGEHKLIPIFERELVRRLSF 125
           IP++ Y+QL+PYI RIA+GDDS ILC+ PITEFF S+GTSG EHKLIP++E+E VRRLSF
Sbjct: 66  IPLVYYQQLRPYIARIADGDDSSILCSNPITEFFKSSGTSGEEHKLIPMYEQEFVRRLSF 125

Query: 126 FNYVMPRMKELFSN-NFHKGKEMYFLFARVELRTKAGIVARVASTSFCKR-SNSNYILSE 185
           F+Y+ PRMK+LF N N+HK K + F FA+ E +TK GIV R   T+  KR SN   + S 
Sbjct: 126 FSYITPRMKQLFPNINWHKAKGLNFHFAKPEFKTKGGIVVRSIFTNLHKRSSNLRSMPSG 185

Query: 186 NNTSPKDIIQCTDSYQSLYSQLLCGLYQNEIVFRVGALFASTLAHVFKFLENHWVLLAND 245
           NNTSP DII CTDSYQSLY QLLCGLYQN++VFRV ALFASTL HVFKFLENHW+ LA D
Sbjct: 186 NNTSPDDIILCTDSYQSLYCQLLCGLYQNDVVFRVSALFASTLIHVFKFLENHWIDLATD 245

Query: 246 IRTGTLNPTITNSSVRESVMNI-LKPNPQLADFIETECSRGTWEGIIKRLWPNTKFIDAI 305
           IRT T+NP IT+SSVRESVM I +KPNP++AD IE EC +G WEGII RLWPN K+I+A+
Sbjct: 246 IRTRTINPKITSSSVRESVMKIVVKPNPEVADLIENECGKGRWEGIITRLWPNAKYINAV 305

Query: 306 VTGSMSQYIPLLNYYSNDLPLVSNHYCSSECFFGLNLDPLCKPEEISYTLIPTMAYFEFL 365
           VTGSMSQYIPLLNYY+N+LP+VS+HY SSECF GLNLDPLC P+E+SYTLIPTMAYFEFL
Sbjct: 306 VTGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCNPDEVSYTLIPTMAYFEFL 365

Query: 366 PIDRTKANGEVRQELVDLIDVKLGQEYELVITTYAGLYRYCMGDILRVTGFKNKAPKFSF 425
           PID     GEV Q LVDL+DVKLG EYELVITT+AGLYRYCMGD++RVTGFKNKAP FSF
Sbjct: 366 PIDMLNPKGEVNQRLVDLVDVKLGHEYELVITTFAGLYRYCMGDVVRVTGFKNKAPNFSF 425

Query: 426 VCRKSAGLSIDAEKTDEAELQNAIQEAAHILSPFGATIVEYTSYADSSTIPGHYVLYMEI 485
           V RK+  L+I  EKTDEA L  A++E   ++  FGATIV+YT+YAD STIPGHYVLY E+
Sbjct: 426 VRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKQFGATIVDYTTYADISTIPGHYVLYWEL 485

Query: 486 SFK--KEVS---IPSSIYEDYCLAIEESLSRLYRVARSHEKCINPLEIRIVEVGTFDKLM 545
           +    KE +   IP S++ED CLAIE SL+ LYR+ARSHEKCINPLEI+IV+ GTF+KLM
Sbjct: 486 TLDDLKEQNLNYIPCSVFEDCCLAIENSLNLLYRLARSHEKCINPLEIKIVKAGTFEKLM 545

Query: 546 EQALNRGASMSQYKTPRCVNSKQ--MIELLDSNVASNYFSKKCPKLD 583
           + ALNRGAS++QYKTPRC+NS Q  +I+LL+SN+ SNYFSK  P L+
Sbjct: 546 QLALNRGASITQYKTPRCLNSSQIHIIQLLESNLVSNYFSKNYPNLE 592

[Close]

BLAST of Lag0009578 vs. ExPASy TrEMBL
Match: A0A1S3BJT8 (indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucumis melo OX=3656 GN=LOC103490488 PE=3 SV=1)

HSP 1 Score: 817.0 bits (2109), Expect = 5.1e-233
Identity = 407/587 (69.34%), Postives = 485/587 (82.62%), Query Frame = 0


Query: 6   ENLMDEKDKQALQHIEDVTSNAEETQQQILAEILSTNAHVEYLQQHGLNGSTDVSTFKKL 65
           E  +DEKDK+ALQ+IED+T+ A+E Q+QIL EILS NA+VEYLQ+HGL+ STD STFKKL
Sbjct: 6   EKWLDEKDKEALQYIEDITTKADEIQRQILNEILSKNANVEYLQKHGLDASTDSSTFKKL 65

Query: 66  IPIISYEQLKPYIERIANGDDSPILCAEPITEFFISTGTSGGEHKLIPIFERELVRRLSF 125
           IP++ Y+QL+PYI RIA+GDDS ILC+ PITEFF S+GTSG EHKLIP++E+E VRRLSF
Sbjct: 66  IPLVYYQQLRPYIARIADGDDSSILCSNPITEFFKSSGTSGEEHKLIPMYEQEFVRRLSF 125

Query: 126 FNYVMPRMKELFSN-NFHKGKEMYFLFARVELRTKAGIVARVASTSFCKR-SNSNYILSE 185
           F+Y+ PRMK+LF N N+HK K + F FA+ E +TK GIV R   T+  KR SN   + S 
Sbjct: 126 FSYITPRMKQLFPNINWHKAKGLNFHFAKPEFKTKGGIVVRSIFTNLHKRSSNLRSMPSG 185

Query: 186 NNTSPKDIIQCTDSYQSLYSQLLCGLYQNEIVFRVGALFASTLAHVFKFLENHWVLLAND 245
           NNTSP DII CTDSYQSLY QLLCGLYQN++VFRV ALFASTL HVFKFLENHW+ LA D
Sbjct: 186 NNTSPDDIILCTDSYQSLYCQLLCGLYQNDVVFRVSALFASTLIHVFKFLENHWIDLATD 245

Query: 246 IRTGTLNPTITNSSVRESVMNI-LKPNPQLADFIETECSRGTWEGIIKRLWPNTKFIDAI 305
           IRT T+NP IT+SSVRESVM I +KPNP++AD IE EC +G WEGII RLWPN K+I+A+
Sbjct: 246 IRTRTINPKITSSSVRESVMKIVVKPNPEVADLIENECGKGRWEGIITRLWPNAKYINAV 305

Query: 306 VTGSMSQYIPLLNYYSNDLPLVSNHYCSSECFFGLNLDPLCKPEEISYTLIPTMAYFEFL 365
           VTGSMSQYIPLLNYY+N+LP+VS+HY SSECF GLNLDPLC P+E+SYTLIPTMAYFEFL
Sbjct: 306 VTGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCNPDEVSYTLIPTMAYFEFL 365

Query: 366 PIDRTKANGEVRQELVDLIDVKLGQEYELVITTYAGLYRYCMGDILRVTGFKNKAPKFSF 425
           PID     GEV Q LVDL+DVKLG EYELVITT+AGLYRYCMGD++RVTGFKNKAP FSF
Sbjct: 366 PIDMLNPKGEVNQRLVDLVDVKLGHEYELVITTFAGLYRYCMGDVVRVTGFKNKAPNFSF 425

Query: 426 VCRKSAGLSIDAEKTDEAELQNAIQEAAHILSPFGATIVEYTSYADSSTIPGHYVLYMEI 485
           V RK+  L+I  EKTDEA L  A++E   ++  FGATIV+YT+YAD STIPGHYVLY E+
Sbjct: 426 VRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKQFGATIVDYTTYADISTIPGHYVLYWEL 485

Query: 486 SFK--KEVS---IPSSIYEDYCLAIEESLSRLYRVARSHEKCINPLEIRIVEVGTFDKLM 545
           +    KE +   IP S++ED CLAIE SL+ LYR+ARSHEKCINPLEI+IV+ GTF+KLM
Sbjct: 486 TLDDLKEQNLNYIPCSVFEDCCLAIENSLNLLYRLARSHEKCINPLEIKIVKAGTFEKLM 545

Query: 546 EQALNRGASMSQYKTPRCVNSKQ--MIELLDSNVASNYFSKKCPKLD 583
           + ALNRGAS++QYKTPRC+NS Q  +I+LL+SN+ SNYFSK  P L+
Sbjct: 546 QLALNRGASITQYKTPRCLNSSQIHIIQLLESNLVSNYFSKNYPNLE 592

[Close]

BLAST of Lag0009578 vs. ExPASy TrEMBL
Match: A0A0A0KAF9 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G125240 PE=3 SV=1)

HSP 1 Score: 815.8 bits (2106), Expect = 1.1e-232
Identity = 410/590 (69.49%), Postives = 486/590 (82.37%), Query Frame = 0


Query: 6   ENLMDEKDKQALQHIEDVTSNAEETQQQILAEILSTNAHVEYLQQHGLNGSTDVSTFKKL 65
           E  +DEK+K+ALQ+IED+T+ A+E Q+QIL EILSTNA+VEYLQQHGL   TD  TFKKL
Sbjct: 6   EKWLDEKEKEALQYIEDITTKADEIQRQILNEILSTNANVEYLQQHGLVVPTDSPTFKKL 65

Query: 66  IPIISYEQLKPYIERIANGDDSPILCAEPITEFFISTGTSGGEHKLIPIFERELVRRLSF 125
           IP++ YEQL+PYI RIA+GDDS ILC+ PITEFF S+GTSGGEHKLIP++E+E VRRLSF
Sbjct: 66  IPLVCYEQLRPYIARIADGDDSSILCSNPITEFFKSSGTSGGEHKLIPMYEQEFVRRLSF 125

Query: 126 FNYVMPRMKELFSN-NFHKGKEMYFLFARVELRTKAGIVARVASTSFCKR-SNSNYILSE 185
           F+Y+MPRMK+LF + N+HK K + F FA+ E +TK GI+ R   T+  KR SN   + S 
Sbjct: 126 FSYIMPRMKQLFPDINWHKVKGLNFHFAKPEFKTKGGIIVRSIFTNLHKRSSNLESMPSG 185

Query: 186 NNTSPKDIIQCTDSYQSLYSQLLCGLYQNEIVFRVGALFASTLAHVFKFLENHWVLLAND 245
           NNTSP DII CTDSYQSLY QLLCGLYQNE+VFRV ALFASTL HVFKFLENHWV LA D
Sbjct: 186 NNTSPDDIILCTDSYQSLYCQLLCGLYQNEVVFRVSALFASTLIHVFKFLENHWVDLATD 245

Query: 246 IRTGTLNPTITNSSVRESVMNIL-KPNPQLADFIETECSRGTWEGIIKRLWPNTKFIDAI 305
           IRT T+NP ITNSSVRES+M I+ KPNP++AD IE EC +G WEGII RLWPN K+I+AI
Sbjct: 246 IRTRTVNPKITNSSVRESLMKIIVKPNPEVADLIENECRKGRWEGIITRLWPNAKYINAI 305

Query: 306 VTGSMSQYIPLLNYYSNDLPLVSNHYCSSECFFGLNLDPLCKPEEISYTLIPTMAYFEFL 365
           VTGSMSQYIPLLNYY+N+LP+VS+HY SSECF GLNLDPLC  +E+SYTLIPTMAYFEFL
Sbjct: 306 VTGSMSQYIPLLNYYTNNLPIVSDHYGSSECFLGLNLDPLCNHDEVSYTLIPTMAYFEFL 365

Query: 366 PIDR-TKANGEVRQELVDLIDVKLGQEYELVITTYAGLYRYCMGDILRVTGFKNKAPKFS 425
           PID     NGEV Q+LVDL+DVKLG+EYELVITT+AGLYRYCMGDI+RVTGFKNKAP F 
Sbjct: 366 PIDMINDPNGEVNQQLVDLVDVKLGREYELVITTFAGLYRYCMGDIVRVTGFKNKAPSFR 425

Query: 426 FVCRKSAGLSIDAEKTDEAELQNAIQEAAHILSPFGATIVEYTSYADSSTIPGHYVLYME 485
           FV RK+  L+I  EKTDEA L  A++E   ++  FGA IV+YT+YAD STIPGHYVLY E
Sbjct: 426 FVRRKNVVLNISNEKTDEAGLHKAVEEGGRVMKSFGAKIVDYTTYADLSTIPGHYVLYWE 485

Query: 486 ISF-----KKEVSIPSSIYEDYCLAIEESLSRLYRVARSHEKCINPLEIRIVEVGTFDKL 545
           ++      +    IPSS++ED CL IE SL+ LYR+ARSHEKCINPLEI+IV+ GTF+KL
Sbjct: 486 LTMDDLKEQNSNDIPSSVFEDCCLDIENSLNLLYRLARSHEKCINPLEIKIVKAGTFEKL 545

Query: 546 MEQALNRGASMSQYKTPRCVNSKQ--MIELLDSNVASNYFSKKCPKLDTN 585
           M+ AL+RGAS++QYKTPRC+NS Q  +I+LL+SNV SNYFS+K P LDTN
Sbjct: 546 MQLALDRGASITQYKTPRCLNSSQIHIIQLLESNVVSNYFSRKYPNLDTN 595

[Close]

BLAST of Lag0009578 vs. TAIR 10
Match: AT4G27260.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 689.9 bits (1779), Expect = 1.8e-198
Identity = 343/586 (58.53%), Postives = 435/586 (74.23%), Query Frame = 0


Query: 9   MDEKDKQALQHIEDVTSNAEETQQQILAEILSTNAHVEYLQQHGLNGSTDVSTFKKLIPI 68
           +D+K+KQ LQ IE++TSNA++ Q+Q+L EIL+ NA VEYL++H LNG TD  TFK ++P+
Sbjct: 17  LDQKNKQKLQLIEELTSNADQVQRQVLEEILTRNADVEYLRRHDLNGRTDRETFKNIMPV 76

Query: 69  ISYEQLKPYIERIANGDDSPILCAEPITEFFISTGTSGGEHKLIPIFERELVRRLSFFNY 128
           I+YE ++P I RIANGD SPIL ++PI+EF  S+GTSGGE KL+P  E EL RR   ++ 
Sbjct: 77  ITYEDIEPEINRIANGDKSPILSSKPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSL 136

Query: 129 VMPRMKELFSNNFHKGKEMYFLFARVELRTKAGIVARVASTSFCKRSNSN---YILSENN 188
           +MP M + F      GK MYFLF + E +T  G+ AR   TS+ K S+     Y    N 
Sbjct: 137 LMPVMSQ-FVPGLENGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKERPYDPYTNY 196

Query: 189 TSPKDIIQCTDSYQSLYSQLLCGLYQNEIVFRVGALFASTLAHVFKFLENHWVLLANDIR 248
           TSP + I C+DSYQS+YSQ+LCGL Q++ V RVGA+FAS      KFLE HW+ L  DIR
Sbjct: 197 TSPNETILCSDSYQSMYSQMLCGLCQHQEVLRVGAVFASGFIRAIKFLEKHWIELVRDIR 256

Query: 249 TGTLNPTITNSSVRESVMNILKPNPQLADFIETECSRGTWEGIIKRLWPNTKFIDAIVTG 308
           TGTL+  IT+ SVRE+V  ILKP+P+LADF+E EC + +W+GII RLWPNTK++D IVTG
Sbjct: 257 TGTLSSLITDPSVREAVAKILKPSPKLADFVEFECKKSSWQGIITRLWPNTKYVDVIVTG 316

Query: 309 SMSQYIPLLNYYSNDLPLVSNHYCSSECFFGLNLDPLCKPEEISYTLIPTMAYFEFLPID 368
           +MSQYIP L+YYSN LPLV   Y SSEC+FG+NL PLCKP E+SYTLIP+MAYFEFLP+ 
Sbjct: 317 TMSQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPSMAYFEFLPVH 376

Query: 369 RTKA-----------NGEVRQELVDLIDVKLGQEYELVITTYAGLYRYCMGDILRVTGFK 428
           R                + +QELVDL+DVKLGQEYELV+TTYAGL RY +GD+LRVTGFK
Sbjct: 377 RNNGVTNSINLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLCRYRVGDLLRVTGFK 436

Query: 429 NKAPKFSFVCRKSAGLSIDAEKTDEAELQNAIQEAAHILSPFGATIVEYTSYADSSTIPG 488
           NKAP+FSF+CRK+  LSID++KTDE ELQNA++ A   L PF A++ EYTSYAD+S+IPG
Sbjct: 437 NKAPQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPG 496

Query: 489 HYVLYMEISFKKEVSIPSSIYEDYCLAIEESLSRLYRVARSHEKCINPLEIRIVEVGTFD 548
           HYVL+ E+       IP S++ED CLA+EES + +YR  R  +K I PLEI+IVE GTFD
Sbjct: 497 HYVLFWELCLDGNTPIPPSVFEDCCLAVEESFNTVYRQGRVSDKSIGPLEIKIVEPGTFD 556

Query: 549 KLMEQALNRGASMSQYKTPRCVNSKQMIELLDSNVASNYFSKKCPK 581
           KLM+ A++ GAS++QYKTPRCV    +IELL+S V  +YFS KCPK
Sbjct: 557 KLMDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPK 601

[Close]

BLAST of Lag0009578 vs. TAIR 10
Match: AT5G54510.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 686.8 bits (1771), Expect = 1.5e-197
Identity = 343/584 (58.73%), Postives = 430/584 (73.63%), Query Frame = 0


Query: 11  EKDKQALQHIEDVTSNAEETQQQILAEILSTNAHVEYLQQHGLNGSTDVSTFKKLIPIIS 70
           EK+K  LQ IEDVT+NA++ Q+++L EILS NA VEYL++HGL G TD  TFK ++P+++
Sbjct: 19  EKNKNKLQFIEDVTTNADDVQRRVLEEILSRNADVEYLKRHGLEGRTDRETFKHIMPVVT 78

Query: 71  YEQLKPYIERIANGDDSPILCAEPITEFFISTGTSGGEHKLIPIFERELVRRLSFFNYVM 130
           YE ++P I RIANGD S +LC+ PI+EF  S+GTSGGE KL+P  E EL RR   ++ +M
Sbjct: 79  YEDIQPEINRIANGDKSQVLCSNPISEFLTSSGTSGGERKLMPTIEEELDRRSLLYSLLM 138

Query: 131 PRMKELFSNNFHKGKEMYFLFARVELRTKAGIVARVASTSFCKRS---NSNYILSENNTS 190
           P M + F     KGK MYFLF + E +T  G+ AR   TS+ K S   N  Y    N TS
Sbjct: 139 PVMDQ-FVPGLDKGKGMYFLFIKSESKTPGGLPARPVLTSYYKSSHFKNRPYDPYTNYTS 198

Query: 191 PKDIIQCTDSYQSLYSQLLCGLYQNEIVFRVGALFASTLAHVFKFLENHWVLLANDIRTG 250
           P   I C+DSYQS+YSQ+LCGL Q++ V RVGA+FAS      KFLE HW  LA DIRTG
Sbjct: 199 PNQTILCSDSYQSMYSQMLCGLCQHKEVLRVGAVFASGFIRAIKFLEKHWPELARDIRTG 258

Query: 251 TLNPTITNSSVRESVMNILKPNPQLADFIETECSRGTWEGIIKRLWPNTKFIDAIVTGSM 310
           TL+  IT+SSVRE+V  ILKP+P+LADF+E+EC + +W+GII RLWPNTK++D IVTG+M
Sbjct: 259 TLSSEITDSSVREAVGEILKPDPKLADFVESECRKTSWQGIITRLWPNTKYVDVIVTGTM 318

Query: 311 SQYIPLLNYYSNDLPLVSNHYCSSECFFGLNLDPLCKPEEISYTLIPTMAYFEFLPIDRT 370
           SQYIP L+YYSN LPLV   Y SSEC+FG+NL PLCKP E+SYTLIP MAYFEFLP+ R 
Sbjct: 319 SQYIPTLDYYSNGLPLVCTMYASSECYFGVNLRPLCKPSEVSYTLIPNMAYFEFLPVHRN 378

Query: 371 KA-----------NGEVRQELVDLIDVKLGQEYELVITTYAGLYRYCMGDILRVTGFKNK 430
                          + +QELVDL+DVKLGQEYELV+TTYAGLYRY +GD+L V GFKN 
Sbjct: 379 SGVTSSISLPKALTEKEQQELVDLVDVKLGQEYELVVTTYAGLYRYRVGDVLSVAGFKNN 438

Query: 431 APKFSFVCRKSAGLSIDAEKTDEAELQNAIQEAAHILSPFGATIVEYTSYADSSTIPGHY 490
           AP+FSF+CRK+  LSID++KTDE ELQNA++ A   L PF A++ EYTSYAD+S+IPGHY
Sbjct: 439 APQFSFICRKNVVLSIDSDKTDEVELQNAVKNAVTHLVPFDASLSEYTSYADTSSIPGHY 498

Query: 491 VLYMEISFKKEVSIPSSIYEDYCLAIEESLSRLYRVARSHEKCINPLEIRIVEVGTFDKL 550
           VL+ E+       IP S++ED CL IEESL+ +YR  R  +K I PLEI++VE GTFDKL
Sbjct: 499 VLFWELCLNGNTPIPPSVFEDCCLTIEESLNSVYRQGRVSDKSIGPLEIKMVESGTFDKL 558

Query: 551 MEQALNRGASMSQYKTPRCVNSKQMIELLDSNVASNYFSKKCPK 581
           M+ A++ GAS++QYKTPRCV    +IELL+S V  +YFS KCPK
Sbjct: 559 MDYAISLGASINQYKTPRCVKFAPIIELLNSRVVDSYFSPKCPK 601

[Close]

BLAST of Lag0009578 vs. TAIR 10
Match: AT2G14960.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 580.9 bits (1496), Expect = 1.2e-165
Identity = 300/574 (52.26%), Postives = 395/574 (68.82%), Query Frame = 0


Query: 11  EKDKQALQHIEDVTSNAEETQQQILAEILSTNAHVEYLQQHGLNGSTDVSTFKKLIPIIS 70
           EKD +AL+ IE++T NA+  Q+ +LAEIL+ NA  EYL++  L G+TD  TFK  IP+I+
Sbjct: 17  EKDAKALRFIEEMTRNADTVQENLLAEILARNADTEYLRRFNLCGATDRDTFKTKIPVIT 76

Query: 71  YEQLKPYIERIANGDDSPILCAEPITEFFISTGTSGGEHKLIPIFERELVRRLSFFNYVM 130
           YE L+P I+RIA+GD SPIL A PI+EF  S+GTS GE KL+P  + EL RR   ++ +M
Sbjct: 77  YEDLQPEIQRIADGDRSPILSAHPISEFLTSSGTSAGERKLMPTIKEELDRRQLLYSLLM 136

Query: 131 PRMKELFSNNFHKGKEMYFLFARVELRTKAGIVARVASTSFCKRSNSN---YILSENNTS 190
           P M  L+     KGK MYFLF + E +T  G+ AR   TS+ K  +     Y      TS
Sbjct: 137 PVM-NLYVPGLDKGKGMYFLFVKSETKTPGGLPARPVLTSYYKSEHFRSRPYDPYNVYTS 196

Query: 191 PKDIIQCTDSYQSLYSQLLCGLYQNEIVFRVGALFASTLAHVFKFLENHWVLLANDIRTG 250
           P + I C DS+QS+Y+Q+LCGL     V RVGA+FAS L    +FL+ HW   A+DI  G
Sbjct: 197 PNEAILCPDSFQSMYTQMLCGLLDRLSVLRVGAVFASGLLRAIRFLQLHWSRFAHDIELG 256

Query: 251 TLNPTITNSSVRESVMNILKPNPQLADFIETECSRGTWEGIIKRLWPNTKFIDAIVTGSM 310
            L+  IT+ S+R+ +  ILKP+P LA+FI  EC    WE II R+WPNTK++D IVTG+M
Sbjct: 257 CLDSEITDPSIRQCMSGILKPDPVLAEFIRRECKSDNWEKIITRIWPNTKYLDVIVTGAM 316

Query: 311 SQYIPLLNYYSNDLPLVSNHYCSSECFFGLNLDPLCKPEEISYTLIPTMAYFEFLPIDRT 370
           +QYIP L YYS  LP+    Y SSEC+FGLNL+P+ KP E+SYT++P MAYFEF+P+  T
Sbjct: 317 AQYIPTLEYYSGGLPMACTMYASSECYFGLNLNPMSKPSEVSYTIMPNMAYFEFIPLGGT 376

Query: 371 KANGEVRQELVDLIDVKLGQEYELVITTYAGLYRYCMGDILRVTGFKNKAPKFSFVCRKS 430
           KA        V+L+DV +G+EYELV+TTYAGL RY +GDILRVTGF N AP+F FV RK+
Sbjct: 377 KA--------VELVDVNIGKEYELVVTTYAGLCRYRVGDILRVTGFHNSAPQFHFVRRKN 436

Query: 431 AGLSIDAEKTDEAELQNAIQEAAHIL-SPFGATIVEYTSYADSSTIPGHYVLYMEISFKK 490
             LSID++KTDE+ELQ A++ A+ IL    G+ + EYTSYAD+STIPGHYVLY E+  + 
Sbjct: 437 VLLSIDSDKTDESELQKAVENASSILHEECGSRVAEYTSYADTSTIPGHYVLYWELLVRD 496

Query: 491 EVSIPS-SIYEDYCLAIEESLSRLYRVARSHEKCINPLEIRIVEVGTFDKLMEQALNRGA 550
               PS       CL +EESL+ +YR +R  +  + PLEIR+V  GTF++LM+ A++RGA
Sbjct: 497 GARQPSHETLTRCCLGMEESLNSVYRQSRVADNSVGPLEIRVVRNGTFEELMDYAISRGA 556

Query: 551 SMSQYKTPRCVNSKQMIELLDSNVASNYFSKKCP 580
           S++QYK PRCVN   ++ELLDS V S +FS   P
Sbjct: 557 SINQYKVPRCVNFTPIVELLDSRVVSAHFSPSLP 581

[Close]

BLAST of Lag0009578 vs. TAIR 10
Match: AT2G23170.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 564.3 bits (1453), Expect = 1.1e-160
Identity = 289/577 (50.09%), Postives = 402/577 (69.67%), Query Frame = 0


Query: 12  KDKQALQHIEDVTSNAEETQQQILAEILSTNAHVEYLQQHGLNGSTDVSTFKKLIPIISY 71
           KD +AL+ IE++T N +  Q++++ EILS N+  EYL++ GL G TD  TFK  +P++ Y
Sbjct: 18  KDVKALRFIEEMTRNVDFVQKKVIREILSRNSDTEYLKRFGLKGFTDRKTFKTKVPVVIY 77

Query: 72  EQLKPYIERIANGDDSPILCAEPITEFFISTGTSGGEHKLIPIFERELVRRLSFFNYVMP 131
           + LKP I+RIANGD S IL + PITEF  S+GTS GE KL+P  + ++ RR   ++ +MP
Sbjct: 78  DDLKPEIQRIANGDRSMILSSYPITEFLTSSGTSAGERKLMPTIDEDMDRRQLLYSLLMP 137

Query: 132 RMKELFSNNFHKGKEMYFLFARVELRTKAGIVARVASTSFCK------RSNSNYILSENN 191
            M  L+     KGK +YFLF + E +T  G+ AR   TS+ K      R N  Y +    
Sbjct: 138 VM-NLYVPGLDKGKALYFLFVKTESKTPGGLPARPVLTSYYKSEQFKRRPNDPYNV---Y 197

Query: 192 TSPKDIIQCTDSYQSLYSQLLCGLYQNEIVFRVGALFASTLAHVFKFLENHWVLLANDIR 251
           TSP + I C DS QS+Y+Q+LCGL     V R+GA+FAS L     FL+ +W  LA+DI 
Sbjct: 198 TSPNEAILCPDSSQSMYTQMLCGLLMRHEVLRLGAVFASGLLRAIGFLQTNWKELADDIS 257

Query: 252 TGTLNPTITNSSVRESVMNIL-KPNPQLADFIETECSR-GTWEGIIKRLWPNTKFIDAIV 311
           TGTL+  I++ +++ES+  IL KP+ +LADFI + C +  +WEGII ++WPNTK++D IV
Sbjct: 258 TGTLSSRISDPAIKESMSKILTKPDQELADFITSVCGQDNSWEGIITKIWPNTKYLDVIV 317

Query: 312 TGSMSQYIPLLNYYSNDLPLVSNHYCSSECFFGLNLDPLCKPEEISYTLIPTMAYFEFLP 371
           TG+M+QYIP+L YYS  LP+    Y SSE +FG+NL P+CKP E+SYT++P MAYFEFLP
Sbjct: 318 TGAMAQYIPMLEYYSGGLPMACTMYASSESYFGINLKPMCKPSEVSYTIMPNMAYFEFLP 377

Query: 372 IDRTKANGEVRQELVDLIDVKLGQEYELVITTYAGLYRYCMGDILRVTGFKNKAPKFSFV 431
                     + ELV+L DV++G+EYELVITTYAGL RY +GDIL+VTGF N AP+F FV
Sbjct: 378 HHEVPTE---KSELVELADVEVGKEYELVITTYAGLNRYRVGDILQVTGFYNSAPQFKFV 437

Query: 432 CRKSAGLSIDAEKTDEAELQNAIQEAAHILSPFGATIVEYTSYADSSTIPGHYVLYMEIS 491
            RK+  LSI+++KTDEAELQ+A++ A+ +L   G  ++EYTSYA++ TIPGHYV+Y E+ 
Sbjct: 438 RRKNVLLSIESDKTDEAELQSAVENASLLLGEQGTRVIEYTSYAETKTIPGHYVIYWELL 497

Query: 492 FKKEVSIPS-SIYEDYCLAIEESLSRLYRVARSHEKCINPLEIRIVEVGTFDKLMEQALN 551
            K + + P+  +    CL +EESL+ +YR +R  +K I PLEIR+V+ GTF++LM+ A++
Sbjct: 498 VKDQTNPPNDEVMARCCLEMEESLNSVYRQSRVADKSIGPLEIRVVKNGTFEELMDYAIS 557

Query: 552 RGASMSQYKTPRCVNSKQMIELLDSNVASNYFSKKCP 580
           RGAS++QYK PRCV+   ++ELLDS V S +FS   P
Sbjct: 558 RGASINQYKVPRCVSFTPIMELLDSRVVSTHFSPALP 587

[Close]

BLAST of Lag0009578 vs. TAIR 10
Match: AT1G59500.1 (Auxin-responsive GH3 family protein )

HSP 1 Score: 563.9 bits (1452), Expect = 1.5e-160
Identity = 285/576 (49.48%), Postives = 404/576 (70.14%), Query Frame = 0


Query: 11  EKDKQALQHIEDVTSNAEETQQQILAEILSTNAHVEYLQQHGLNGSTDVSTFKKLIPIIS 70
           E + +AL+ IE++T N +  Q+++L EILS N++ EYL++  LNG+ D  +FK  +P++ 
Sbjct: 18  ETEVKALKFIEEITRNPDSVQEKVLGEILSRNSNTEYLKRFDLNGAVDRKSFKSKVPVVI 77

Query: 71  YEQLKPYIERIANGDDSPILCAEPITEFFISTGTSGGEHKLIPIFERELVRRLSFFNYVM 130
           YE LK  I+RI+NGD SPIL + PITEF  S+GTS GE KL+P  E ++ RR    N +M
Sbjct: 78  YEDLKTDIQRISNGDRSPILSSHPITEFLTSSGTSAGERKLMPTIEEDINRRQLLGNLLM 137

Query: 131 PRMKELFSNNFHKGKEMYFLFARVELRTKAGIVARVASTSFCKRSNSNYILSENN----T 190
           P M  L+     KGK +YFLF + E  T  G+ AR A TS+ K   S+Y  + ++    T
Sbjct: 138 PVM-NLYVPGLDKGKGLYFLFVKSESTTSGGLPARPALTSYYK---SDYFRTSDSDSVYT 197

Query: 191 SPKDIIQCTDSYQSLYSQLLCGLYQNEIVFRVGALFASTLAHVFKFLENHWVLLANDIRT 250
           SPK+ I C DS QS+Y+Q+LCGL     V R+GA+F S L     FL+N+W  L+ DI T
Sbjct: 198 SPKEAILCCDSSQSMYTQMLCGLLMRHEVNRLGAVFPSGLLRAISFLQNNWKELSQDIST 257

Query: 251 GTLNPTITNSSVRESVMNIL-KPNPQLADFIETECSRGTWEGIIKRLWPNTKFIDAIVTG 310
           GTL+  I + +++  + NIL KP+ +LA+F+   CS+  WEGII ++WPNTK++D IVTG
Sbjct: 258 GTLSSKIFDHAIKTRMSNILNKPDQELAEFLIGVCSQENWEGIITKIWPNTKYLDVIVTG 317

Query: 311 SMSQYIPLLNYYSNDLPLVSNHYCSSECFFGLNLDPLCKPEEISYTLIPTMAYFEFLPID 370
           +M++YIP+L YYS  LP+ S  Y SSE +FG+NL+P+CKP E+SYT+ P MAYFEFLP +
Sbjct: 318 AMAEYIPMLEYYSGGLPMASMIYASSESYFGINLNPMCKPSEVSYTIFPNMAYFEFLPHN 377

Query: 371 RTKANGEVRQELVDLIDVKLGQEYELVITTYAGLYRYCMGDILRVTGFKNKAPKFSFVCR 430
                G     LV+L DV++G+EYELVITTYAGLYRY +GDILRVTGF N AP+F F+ R
Sbjct: 378 HDGDGGVEATSLVELADVEVGKEYELVITTYAGLYRYRVGDILRVTGFHNSAPQFKFIRR 437

Query: 431 KSAGLSIDAEKTDEAELQNAIQEAAHILSPFGATIVEYTSYADSSTIPGHYVLYME-ISF 490
           ++  LSI+++KTDEA+LQ A++ A+ +L+  G  ++EYTSYAD+ TIPGHYV+Y E +S 
Sbjct: 438 ENVLLSIESDKTDEADLQKAVENASRLLAEQGTRVIEYTSYADTKTIPGHYVIYWELLSR 497

Query: 491 KKEVSIPS-SIYEDYCLAIEESLSRLYRVARSHEKCINPLEIRIVEVGTFDKLMEQALNR 550
            +  ++PS  +    CL +EESL+ +YR +R  +K I PLEIR+V+ GTF++LM+ +++R
Sbjct: 498 DQSNALPSDEVMAKCCLEMEESLNAVYRQSRVSDKSIGPLEIRVVQNGTFEELMDFSISR 557

Query: 551 GASMSQYKTPRCVNSKQMIELLDSNVASNYFSKKCP 580
           G+S++QYK PRCV+   +++LLDS V S +FS   P
Sbjct: 558 GSSINQYKVPRCVSLTPIMKLLDSRVVSAHFSPSLP 589

The following BLAST results are available for this feature:

BLAST of Lag0009578 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6570863.1	4.7e-257	75.17	Indole-3-acetic acid-amido synthetase, partial [Cucurbita argyrosperma subsp. so...	[more]
XP_022944596.1	2.2e-254	74.83	indole-3-acetic acid-amido synthetase GH3.6-like [Cucurbita moschata]	[more]
XP_038902067.1	2.0e-247	72.94	indole-3-acetic acid-amido synthetase GH3.5-like [Benincasa hispida]	[more]
XP_022140778.1	7.3e-242	72.29	indole-3-acetic acid-amido synthetase GH3.6-like [Momordica charantia]	[more]
XP_008448232.1	1.1e-232	69.34	PREDICTED: indole-3-acetic acid-amido synthetase GH3.6-like [Cucumis melo] >KAA0...	[more]

BLAST of Lag0009578 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O81829	2.6e-197	58.53	Indole-3-acetic acid-amido synthetase GH3.5 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
Q9LSQ4	2.2e-196	58.73	Indole-3-acetic acid-amido synthetase GH3.6 OS=Arabidopsis thaliana OX=3702 GN=G...	[more]
Q8LQM5	3.8e-177	53.83	Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Oryza sativa subsp. japo...	[more]
Q60EJ6	1.9e-168	51.25	Probable indole-3-acetic acid-amido synthetase GH3.4 OS=Oryza sativa subsp. japo...	[more]
O82333	1.7e-164	52.26	Probable indole-3-acetic acid-amido synthetase GH3.1 OS=Arabidopsis thaliana OX=...	[more]

BLAST of Lag0009578 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FUU5	1.1e-254	74.83	indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucurbita moschata OX=3662 G...	[more]
A0A6J1CIS9	3.5e-242	72.29	indole-3-acetic acid-amido synthetase GH3.6-like OS=Momordica charantia OX=3673 ...	[more]
A0A5A7V3L5	5.1e-233	69.34	Indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucumis melo var. makuwa OX=...	[more]
A0A1S3BJT8	5.1e-233	69.34	indole-3-acetic acid-amido synthetase GH3.6-like OS=Cucumis melo OX=3656 GN=LOC1...	[more]
A0A0A0KAF9	1.1e-232	69.49	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_6G125240 PE=3 SV=1	[more]

BLAST of Lag0009578 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G27260.1	1.8e-198	58.53	Auxin-responsive GH3 family protein	[more]
AT5G54510.1	1.5e-197	58.73	Auxin-responsive GH3 family protein	[more]
AT2G14960.1	1.2e-165	52.26	Auxin-responsive GH3 family protein	[more]
AT2G23170.1	1.1e-160	50.09	Auxin-responsive GH3 family protein	[more]
AT1G59500.1	1.5e-160	49.48	Auxin-responsive GH3 family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004993	GH3 family	PFAM	PF03321	GH3	coord: 20..559 e-value: 4.4E-187 score: 623.1
IPR004993	GH3 family	PANTHER	PTHR31901	GH3 DOMAIN-CONTAINING PROTEIN	coord: 9..581
None	No IPR available	PANTHER	PTHR31901:SF63	INDOLE-3-ACETIC ACID-AMIDO SYNTHETASE	coord: 9..581

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0009578.1	Lag0009578.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0010279	indole-3-acetic acid amido synthetase activity