Lag0009492 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0009492
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionReverse transcriptase domain-containing protein
Locationchr9: 39688131 .. 39689244 (-)
RNA-Seq ExpressionLag0009492
SyntenyLag0009492
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGGTGGAGGACCGTCTGATTTGGCATTTTGAGGAAAATGACCCTTTTTCTGTTAGAAGCAGATATCGTTTGGCTCTCAAGTTGAAGTCTCAAGGGCTCCCATCATCTTCAGACATGACCCAAGGGCGTGTTTGGTGGTCCAGTTTATGGAAGTTGAATCTGCCTTCCAAGGTAAAGCTCTTTCTATGGAGATTAGCCCGTAATCGTTTGCCTACCAAGGACAACTTGATTAAAAGAGGTATAGATGTCACAAACTTGTGTGTGATATGTCACTCTTCTCTTGAAGATTGTTTACATGTGTTTTGGAAATGTTCTGTTGTTAAAAGCATGTGGCTAGGATCTAAGTTTGCTTCATTATATTTCTCTGTTATGGGTCCTAACTTTGATTTTGTACTATGGGCGGTTAAGGAGAAGCTGTGTATTCATGACTTTGAAATTGTTGTTGTATTTTGGTGGGTTGTTTGGAATCTACATAATGCAGTATTGTGGAACGGGTCGGTAAATGAAAGGGATTTATTTGATTGGGCTTCTGTCTATTTGTCTTCGTTCCAGCAAGCAGCATCGTCGAATGGGGCAGTGGTTGCGGTGGTAGAAGATGGCCAGTCGAGAGGCACGGTGGCTTGCGTGTGGAGGCCGCTTGATGTGGATGAACTGAAGTTGAATATTGATGCGCCTGTTAGACCAGAGTGTGGGACTGTTGGGGGAGGTGCTGTTTTGAGAAACACGAGGGGTGAGGTATTTTTTTCTGCGTGCTTTACTCTCCCCAATTGTTGGAATGTTGATTTTGCGGAGGCTTGGGCGATGCTGAAGGGTATCCAAACTGCTCGTAAGATGGGATTCCGTTGGTTCAGTGTAGAAACCGATTCCCTGAGGTTAGTCAAGCTTTTAAAATGAGAAGGTGGAGGATGTTTCAGAAGTGGGTGCTATGGCGGGAGTGATTCGTGAGCTCCTCCATCAGGTTTCTCCCTGTAGTGTTCTTTTCACACCTAGGAAAGGCAATATGACTGCTCATGTAATGACATCTCTAGCGTTTACTTATGCAAATTTTGTTTGGCTTGAAGAGTGGCCTAGTGAGGTCACTGATGTAATATGTCGTGAGGTTTCTCTTTAA

mRNA sequence

ATGTTGGTGGAGGACCGTCTGATTTGGCATTTTGAGGAAAATGACCCTTTTTCTGTTAGAAGCAGATATCGTTTGGCTCTCAAGTTGAAGTCTCAAGGGCTCCCATCATCTTCAGACATGACCCAAGGGCGTGTTTGGTGGTCCAGTTTATGGAAGTTGAATCTGCCTTCCAAGGTAAAGCTCTTTCTATGGAGATTAGCCCGTAATCGTTTGCCTACCAAGGACAACTTGATTAAAAGAGGTATAGATGTCACAAACTTGTGTGTGATATGTCACTCTTCTCTTGAAGATTGTTTACATGTGTTTTGGAAATGTTCTGTTGTTAAAAGCATGTGGCTAGGATCTAAGTTTGCTTCATTATATTTCTCTGTTATGGGTCCTAACTTTGATTTTGTACTATGGGCGGTTAAGGAGAAGCTGTGTATTCATGACTTTGAAATTGTTGTTGTATTTTGGTGGGTTGTTTGGAATCTACATAATGCAGTATTGTGGAACGGGTCGGTAAATGAAAGGGATTTATTTGATTGGGCTTCTGTCTATTTGTCTTCGTTCCAGCAAGCAGCATCGTCGAATGGGGCAGTGGTTGCGGTGGTAGAAGATGGCCAGTCGAGAGGCACGGTGGCTTGCGTGTGGAGGCCGCTTGATGTGGATGAACTGAAGTTGAATATTGATGCGCCTGTTAGACCAGAGTGTGGGACTGTTGGGGGAGGTGCTGTTTTGAGAAACACGAGGGGTGAGGTATTTTTTTCTGCGTGCTTTACTCTCCCCAATTGTTGGAATGTTGATTTTGCGGAGGCTTGGGCGATGCTGAAGGGTATCCAAACTGCTCGTAAGATGGGATTCCGTTGGTTCAGTGTAGAAACCGATTCCCTGAGGAAAGGCAATATGACTGCTCATGTAATGACATCTCTAGCGTTTACTTATGCAAATTTTGTTTGGCTTGAAGAGTGGCCTAGTGAGGTCACTGATGTAATATGTCGTGAGGTTTCTCTTTAA

Coding sequence (CDS)

ATGTTGGTGGAGGACCGTCTGATTTGGCATTTTGAGGAAAATGACCCTTTTTCTGTTAGAAGCAGATATCGTTTGGCTCTCAAGTTGAAGTCTCAAGGGCTCCCATCATCTTCAGACATGACCCAAGGGCGTGTTTGGTGGTCCAGTTTATGGAAGTTGAATCTGCCTTCCAAGGTAAAGCTCTTTCTATGGAGATTAGCCCGTAATCGTTTGCCTACCAAGGACAACTTGATTAAAAGAGGTATAGATGTCACAAACTTGTGTGTGATATGTCACTCTTCTCTTGAAGATTGTTTACATGTGTTTTGGAAATGTTCTGTTGTTAAAAGCATGTGGCTAGGATCTAAGTTTGCTTCATTATATTTCTCTGTTATGGGTCCTAACTTTGATTTTGTACTATGGGCGGTTAAGGAGAAGCTGTGTATTCATGACTTTGAAATTGTTGTTGTATTTTGGTGGGTTGTTTGGAATCTACATAATGCAGTATTGTGGAACGGGTCGGTAAATGAAAGGGATTTATTTGATTGGGCTTCTGTCTATTTGTCTTCGTTCCAGCAAGCAGCATCGTCGAATGGGGCAGTGGTTGCGGTGGTAGAAGATGGCCAGTCGAGAGGCACGGTGGCTTGCGTGTGGAGGCCGCTTGATGTGGATGAACTGAAGTTGAATATTGATGCGCCTGTTAGACCAGAGTGTGGGACTGTTGGGGGAGGTGCTGTTTTGAGAAACACGAGGGGTGAGGTATTTTTTTCTGCGTGCTTTACTCTCCCCAATTGTTGGAATGTTGATTTTGCGGAGGCTTGGGCGATGCTGAAGGGTATCCAAACTGCTCGTAAGATGGGATTCCGTTGGTTCAGTGTAGAAACCGATTCCCTGAGGAAAGGCAATATGACTGCTCATGTAATGACATCTCTAGCGTTTACTTATGCAAATTTTGTTTGGCTTGAAGAGTGGCCTAGTGAGGTCACTGATGTAATATGTCGTGAGGTTTCTCTTTAA

Protein sequence

MLVEDRLIWHFEENDPFSVRSRYRLALKLKSQGLPSSSDMTQGRVWWSSLWKLNLPSKVKLFLWRLARNRLPTKDNLIKRGIDVTNLCVICHSSLEDCLHVFWKCSVVKSMWLGSKFASLYFSVMGPNFDFVLWAVKEKLCIHDFEIVVVFWWVVWNLHNAVLWNGSVNERDLFDWASVYLSSFQQAASSNGAVVAVVEDGQSRGTVACVWRPLDVDELKLNIDAPVRPECGTVGGGAVLRNTRGEVFFSACFTLPNCWNVDFAEAWAMLKGIQTARKMGFRWFSVETDSLRKGNMTAHVMTSLAFTYANFVWLEEWPSEVTDVICREVSL
Homology
BLAST of Lag0009492 vs. NCBI nr
Match: XP_022150918.1 (uncharacterized protein LOC111018954 [Momordica charantia])

HSP 1 Score: 201.4 bits (511), Expect = 1.2e-47
Identity = 119/348 (34.20%), Postives = 178/348 (51.15%), Query Frame = 0

Query: 4    EDRLIWHFEENDPFSVRSRYRLA-LKLKSQGLPSSSDMTQGRVWWSSLWKLNLPSKVKLF 63
            EDRLIW++E+   +SVRS Y++A L       PSSS   + R WW+  WK+++P+K+K+F
Sbjct: 792  EDRLIWNYEKTGVYSVRSGYKVALLNNPCVQAPSSSSSEEVRCWWNGFWKMHIPNKIKVF 851

Query: 64   LWRLARNRLPTKDNLIKRGIDVTNLCVICHSSLEDCLHVFWKCSVVKSMWLGSKFASLYF 123
            LWRL  +RLPT  NL KRG+++TN C  C  + ED +H+FW C   +++W+ SKF  L  
Sbjct: 852  LWRLCLDRLPTGCNLSKRGVEITNCCYFCGRNGEDSIHLFWICKFAEALWINSKFGKL-- 911

Query: 124  SVMGPNFDFVLWAVKEKLCIHDFEIVVVFWWVVWNLHNAVLWNGSVNE-----RDLFDWA 183
                 +   +L    E L   DFE + V  W +WN  NA  +N S         +L +WA
Sbjct: 912  -----SPFLILRESHESLSKADFEELCVVIWGLWNQRNARAFNDSTKTVFKIGMELVEWA 971

Query: 184  SVYLSSFQQAASSNGAVVAVVEDGQSRGTVACVWRPLDVDELKLNIDAPVRPECGTVGGG 243
            + Y   F++A S+          G+   T   +W+P D    K+N DA         G G
Sbjct: 972  NKYAMEFREAKSN-------PITGRVTNTAEILWQPPDEGIYKINTDASFLASDQHAGLG 1031

Query: 244  AVLRNTRGEVFFSACFTLPNCWNVDFAEAWAMLKGIQTARKMG---------------FR 303
             ++ N RG+V  +A   L N  +VD AEA A ++G+Q A ++G                +
Sbjct: 1032 IIIHNDRGQVMAAATKYLENIQSVDMAEAIAAVEGLQLASEIGMHPALEDLSETGEIVLK 1091

Query: 304  WFSVETDSL--------RKGNMTAHVMTSLAFTYANF-VWLEEWPSEV 322
              +  T SL        R+GN  AH++   A     F +W+E+WP E+
Sbjct: 1092 AKNFWTQSLHASFNFVKREGNKAAHMLARRALLLHEFSIWMEDWPLEL 1125

BLAST of Lag0009492 vs. NCBI nr
Match: XP_030941688.1 (uncharacterized protein LOC115966628 [Quercus lobata])

HSP 1 Score: 164.9 bits (416), Expect = 1.2e-36
Identity = 101/294 (34.35%), Postives = 147/294 (50.00%), Query Frame = 0

Query: 3    VEDRLIWHFEENDPFSVRSRYRLALK-LKSQGLPSSSDMTQGRVWWSSLWKLNLPSKVKL 62
            V D L+W   ++  +SVRS Y LA + L+S      S    GR  W  LWKL +P+K+K+
Sbjct: 819  VSDSLLWLHTKDGRYSVRSGYYLARQILRSDNWAECSSGCSGRDVWEKLWKLKVPNKIKV 878

Query: 63   FLWRLARNRLPTKDNLIKRGIDVTNLCVICHSSLEDCLHVFWKCSVVKSMWLGSKFASLY 122
            + WR  ++ LPT+ NL +R I     C +C ++LE  +H  W+C V + +W GS    L 
Sbjct: 879  YGWRACQDILPTRANLARRKILEDEHCEVCKTALETGIHALWECPVAQDVWSGS-LVRLQ 938

Query: 123  FSVMGPNFDFVLWAVKE---KLCIHDFEIVVVFWWVVWNLHNAVLWNGSVNE-RDLFDWA 182
                G  +D VL    E   +L   +FE+ +   WV+WN  NAV+  G + +   L   A
Sbjct: 939  KCTQG--YDDVLQLFGELLNRLSREEFELFLTLSWVIWNQRNAVIHGGKIKDPGQLCKRA 998

Query: 183  SVYLSSFQQAASSNGAVVAVVEDGQSRGTVACVWRPLDVDELKLNIDAPVRPECGTVGGG 242
             VYL  ++QA               SR      W+P  +D  KLN DA +  E G  G G
Sbjct: 999  EVYLLEYRQAQDQLSI--------PSRQQSVTQWQPPPIDRYKLNFDAAIFTELGCSGFG 1058

Query: 243  AVLRNTRGEVFFSACFTLPNCWNVDFAEAWAMLKGIQTARKMGFRWFSVETDSL 292
            A++RN RGEV  +     P  WN + AEA A  + ++ A   GF    +E D++
Sbjct: 1059 AIVRNERGEVMAALSVKGPLVWNSEEAEALACRRAVEFAIHAGFAELMIEGDNV 1101

BLAST of Lag0009492 vs. NCBI nr
Match: XP_023904177.1 (uncharacterized protein LOC112015942 [Quercus suber])

HSP 1 Score: 153.7 bits (387), Expect = 2.8e-33
Identity = 94/291 (32.30%), Postives = 144/291 (49.48%), Query Frame = 0

Query: 3   VEDRLIWHFEENDPFSVRSRYRLALK-LKSQGLPSSSDMTQGRVWWSSLWKLNLPSKVKL 62
           V+D +IW   +N  +SV+S Y +A + L+S+     S     +  W +LWK+N+P+K+K+
Sbjct: 147 VDDSIIWLHNKNGRYSVKSGYYVARQVLRSENWAECSKGNDEQQVWKALWKMNVPNKIKI 206

Query: 63  FLWRLARNRLPTKDNLIKRGIDVTNLCVICHSSLEDCLHVFWKCSVVKSMWLGSKFASLY 122
           F WR  +  LPT+ NL KR I     C IC  S E+ +HV W+C V + +W GS+     
Sbjct: 207 FGWRACQGILPTRLNLAKRKIIAETFCPICLQSPENEMHVIWECPVAQDVWAGSRIKLQK 266

Query: 123 FSVMGPNFDFVLWAVKEKLCIHDFEIVVVFWWVVWNLHNAVLWNGSVNERDLFDWASV-Y 182
             +  P+   +   +  +L   D  + +V  W++W+  N+VL  G + E    +   V +
Sbjct: 267 CPLGQPDMVQLFLYLLHRLEPEDMALFLVQAWMLWSQRNSVLHGGKLKEPGWLNKRDVEW 326

Query: 183 LSSFQQAASSNGAVVAVVEDGQSRGTVACVWRPLDVDELKLNIDAPVRPECGTVGGGAVL 242
           L  F Q       +       Q R TV  VWRP      K+N DA +  + G  G GAV+
Sbjct: 327 LEEFAQVQDQLDVL-------QPRATVGNVWRPPAHPFFKMNFDAAIFNDSGRSGFGAVI 386

Query: 243 RNTRGEVFFSACFTLPNCWNVDFAEAWAMLKGIQTARKMGFRWFSVETDSL 292
           RN  GEV  +     P+  + D AE  A  K ++ A   GF    +E DS+
Sbjct: 387 RNELGEVMAALSAIGPSVSSSDEAETLACRKAMEFAVDAGFNELIIEGDSI 430

BLAST of Lag0009492 vs. NCBI nr
Match: XP_024956542.1 (uncharacterized protein LOC112498908 [Citrus sinensis])

HSP 1 Score: 149.8 bits (377), Expect = 4.1e-32
Identity = 90/290 (31.03%), Postives = 143/290 (49.31%), Query Frame = 0

Query: 2    LVEDRLIWHFEENDPFSVRSRYRLALKLKSQGLPSSSDMTQGRVWWSSLWKLNLPSKVKL 61
            L ED LIWHF ++  ++V+S Y+ ALK++   +PSSS+ ++    W+ +W L LP K+++
Sbjct: 766  LREDELIWHFGKSGQYTVKSGYQRALKIRFPAMPSSSESSKNE--WNIIWSLALPKKIRI 825

Query: 62   FLWRLARNRLPTKDNLIKRGIDVTNLCVICHSSLEDCLHVFWKCSVVKSMWLGSKFASLY 121
            F+WR A+N LP+ +NL KR I     C +C   +E+  H    C   K +W  S F    
Sbjct: 826  FVWRAAKNLLPSAENLWKRKIVQEPTCQLCKMGIENVFHALVDCKAAKKVWRLSLFYIDI 885

Query: 122  FSVMGPNFDFVLWAVKEKLCIHDFEIVVVFWWVVWNLHNAVLWNGS-VNERDLFDWASVY 181
             +  G +   +L  VK      D ++  V  W  WN  N  L+ G   N + +   A   
Sbjct: 886  QAAPGQDILSLLHGVKRMRSNADVDLFAVMLWAKWNARNQWLFKGKRENPQSVVAKAEAV 945

Query: 182  LSSFQQAASSNGAVVAVVEDGQSRGTVACVWRPLDVDELKLNIDAPVRPECGTVGGGAVL 241
            + ++++   S     A V  G+ +      W P     +K+N DA    E    G GAV+
Sbjct: 946  MEAYKRVQPS-----ADVSHGKQQKVAQLGWNPPQEGFVKINTDAATNSEKNLAGLGAVI 1005

Query: 242  RNTRGEVFFSACFTLPNCWNVDFAEAWAMLKGIQTARKMGFRWFSVETDS 291
            R+  G+V  +A        +V +AEA AM  G+Q A+    +   +E+DS
Sbjct: 1006 RDENGQVTATAIKVSKFHGSVAYAEAEAMEWGLQVAKDAHVKDVIMESDS 1048

BLAST of Lag0009492 vs. NCBI nr
Match: XP_023908235.1 (uncharacterized protein LOC112019924 [Quercus suber])

HSP 1 Score: 148.7 bits (374), Expect = 9.0e-32
Identity = 107/372 (28.76%), Postives = 165/372 (44.35%), Query Frame = 0

Query: 3    VEDRLIWHFEENDPFSVRSRYRLA--LKLKSQGLPSSSDMTQGRVWWSSLWKLNLPSKVK 62
            V D + W F ++  F+V+S Y++A  L  K     SSS     RV W +LWKL +P+K+K
Sbjct: 1230 VSDSVYWKFNKDGIFTVKSAYKVARTLSKKEDWAESSSGSGDSRV-WVALWKLRIPNKIK 1289

Query: 63   LFLWRLARNRLPTKDNLIKRGIDVTNLCVICHSSLEDCLHVFWKCSVVKSMWLGSKFASL 122
            +F WR   + LPT+ NL K+ I V +LC +C  + E  +H  W+C++ + +W GS  A  
Sbjct: 1290 VFGWRACHDILPTRRNLRKKRILVDDLCPLCGLTQESTIHALWECTIAQDIWHGSGRALQ 1349

Query: 123  YFSVMGPNFDFVLWAVKEKLCIHDFEIVVVFWWVVWNLHNAVLWNGSVNERDLFDWASV- 182
                   +F  +L  + ++L   + E  +V  W++WN  N VL+ G + E    +  +V 
Sbjct: 1350 KCGTGQLDFVALLEYLLDRLNKTEVETFLVQAWLIWNQRNRVLYGGKLMEPGCLNRRAVG 1409

Query: 183  YLSSFQQAASSNGAVVAVVEDGQSRGTVACVWRPLDVDELKLNIDAPVRPECGTVGGGAV 242
             +  FQQ+ +S      +  + Q   T    W P      KLN DA V     + G GAV
Sbjct: 1410 LIEEFQQSQASLQVDAQMGVNRQVMQTDGTRWIPPPQAVYKLNYDAAVFENSASSGFGAV 1469

Query: 243  LRNTRGEVFFSACFTLPNCWNVDFAEAWAMLKGIQTARKMGFRWFSVETDSL-------- 302
            +RN+ G+V  +     P     + AE  A  K ++ A   GF    VE DS+        
Sbjct: 1470 VRNSTGDVMAAMTVKGPAVQGSEVAELLACRKALEFAIDSGFTVLIVEGDSVNATKCIAS 1529

Query: 303  -------------------------------RKGNMTAHVMTSLAFTY-ANFVWLEEWPS 332
                                           R GN  A V+   A+    +  W+EE PS
Sbjct: 1530 GSDIQSAIGHVVGDIRHLLRALEWTSVRCTKRNGNRVADVLARYAYNVNVDLFWMEEVPS 1589

BLAST of Lag0009492 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 3.6e-15
Identity = 78/317 (24.61%), Postives = 130/317 (41.01%), Query Frame = 0

Query: 5   DRLIWHFEENDPFSVRSRYRLALKLKSQGLPSSSDMTQGRVWWSSLWKLNLPSKVKLFLW 64
           DRL W F ++  FSVRS Y + L +     P+ +       +++ LWK+ +P +VK FLW
Sbjct: 253 DRLSWKFSQDGQFSVRSAYEM-LTVDEVPRPNMAS------FFNCLWKVRVPERVKTFLW 312

Query: 65  RLARNRLPTKDNLIKRGIDVTNLCVICHSSLEDCLHVFWKCSVVKSMW---LGSKFASLY 124
            +    + T++   +R +  +N+C +C   +E  LHV   C     +W   +  +    +
Sbjct: 313 LVGNQAVMTEEERHRRHLSASNVCQVCKGGVESMLHVLRDCPAQLGIWVRVVPQRRQQGF 372

Query: 125 FSVMGPNFDFVLWAVKEKLCIHDFE-----IVVVFWWVVWNLHNAVLWNGSVNERDLF-- 184
           FS     F+++   + ++    D        V+++W   W   N    N    +R  F  
Sbjct: 373 FS--KSLFEWLYDNLGDRSGCEDIPWSTIFAVIIWWGWKWRCGNIFGENTKCRDRVKFVK 432

Query: 185 DWA-SVYLSSFQQAASSNGAVVAVVEDGQSRGTVACVWRPLDVDELKLNIDAPVRPECGT 244
           +WA  VY       A S   +V +    Q R      W    V  +K+N D   R   G 
Sbjct: 433 EWAVEVY------RAHSGNVLVGIT---QPRVERMIGWVSPCVGWVKVNTDGASRGNPGL 492

Query: 245 VGGGAVLRNTRGEVFFSACFTLPNCWNVDFAEAWAMLKGIQTARKMGFRWFSVETDS--- 304
              G VLR+  G         +  C +   AE W +  G+  A +       +E DS   
Sbjct: 493 ASAGGVLRDCTGAWCGGFSLNIGRC-SAPQAELWGVYYGLYFAWEKKVPRVELEVDSEVI 550

BLAST of Lag0009492 vs. ExPASy TrEMBL
Match: A0A6J1DAR4 (uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018954 PE=4 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 5.7e-48
Identity = 119/348 (34.20%), Postives = 178/348 (51.15%), Query Frame = 0

Query: 4    EDRLIWHFEENDPFSVRSRYRLA-LKLKSQGLPSSSDMTQGRVWWSSLWKLNLPSKVKLF 63
            EDRLIW++E+   +SVRS Y++A L       PSSS   + R WW+  WK+++P+K+K+F
Sbjct: 792  EDRLIWNYEKTGVYSVRSGYKVALLNNPCVQAPSSSSSEEVRCWWNGFWKMHIPNKIKVF 851

Query: 64   LWRLARNRLPTKDNLIKRGIDVTNLCVICHSSLEDCLHVFWKCSVVKSMWLGSKFASLYF 123
            LWRL  +RLPT  NL KRG+++TN C  C  + ED +H+FW C   +++W+ SKF  L  
Sbjct: 852  LWRLCLDRLPTGCNLSKRGVEITNCCYFCGRNGEDSIHLFWICKFAEALWINSKFGKL-- 911

Query: 124  SVMGPNFDFVLWAVKEKLCIHDFEIVVVFWWVVWNLHNAVLWNGSVNE-----RDLFDWA 183
                 +   +L    E L   DFE + V  W +WN  NA  +N S         +L +WA
Sbjct: 912  -----SPFLILRESHESLSKADFEELCVVIWGLWNQRNARAFNDSTKTVFKIGMELVEWA 971

Query: 184  SVYLSSFQQAASSNGAVVAVVEDGQSRGTVACVWRPLDVDELKLNIDAPVRPECGTVGGG 243
            + Y   F++A S+          G+   T   +W+P D    K+N DA         G G
Sbjct: 972  NKYAMEFREAKSN-------PITGRVTNTAEILWQPPDEGIYKINTDASFLASDQHAGLG 1031

Query: 244  AVLRNTRGEVFFSACFTLPNCWNVDFAEAWAMLKGIQTARKMG---------------FR 303
             ++ N RG+V  +A   L N  +VD AEA A ++G+Q A ++G                +
Sbjct: 1032 IIIHNDRGQVMAAATKYLENIQSVDMAEAIAAVEGLQLASEIGMHPALEDLSETGEIVLK 1091

Query: 304  WFSVETDSL--------RKGNMTAHVMTSLAFTYANF-VWLEEWPSEV 322
              +  T SL        R+GN  AH++   A     F +W+E+WP E+
Sbjct: 1092 AKNFWTQSLHASFNFVKREGNKAAHMLARRALLLHEFSIWMEDWPLEL 1125

BLAST of Lag0009492 vs. ExPASy TrEMBL
Match: A0A2N9IZ42 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS58489 PE=4 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 6.1e-34
Identity = 90/284 (31.69%), Postives = 137/284 (48.24%), Query Frame = 0

Query: 5   DRLIWHFEENDPFSVRSRYRLALKLKSQGLPSSSDMTQGRVWWSSLWKLNLPSKVKLFLW 64
           D ++W    N  FS RS Y+L    KS    S+SD ++   +W SLW + +P+K+K+F+W
Sbjct: 441 DSMVWSGTPNGQFSTRSAYQLQAAEKSSLSASTSDQSRNHSFWKSLWGVAVPNKIKVFMW 500

Query: 65  RLARNRLPTKDNLIKRGIDVTNLCVICHSSLEDCLHVFWKCSVVKSMWLGSKFASLYFSV 124
           R  ++ LPTK NL  RG+  +  C +CH   E   H  W C   +++W  S    L++S+
Sbjct: 501 RACKSSLPTKANLFSRGVLSSCTCPVCHDENETIFHTLWDCQYARTVWQNSLLHKLHYSI 560

Query: 125 MGPNFDFVLWAVKEKLCIHDFEIVVVFWWVVW-NLHNAVLWNGSVNERDLFDWASVYLSS 184
              N++ V+  V       D E      W++W N +NA L   S +   L + A+ Y+  
Sbjct: 561 QVSNWNDVVEEVLRTQRQQDIETFFTLAWMIWGNRNNAWLQKPSADAEILGEKAATYVEE 620

Query: 185 FQQAASSNGAVVAVVEDGQSRGTVACVWRPLDVDELKLNIDAPVRPECGTVGGGAVLRNT 244
           + +       V   VED  SR  +   W P     LK+N+         +VG G V+RN+
Sbjct: 621 YNE-------VTKKVED--SRSVLCRKWSPPTGSMLKMNVATTYFNTRKSVGLGVVIRNS 680

Query: 245 RGEVFFSACFTLPNCWNVDFAEAWAMLKGIQTARKMGFRWFSVE 288
           RGE   S C   P+  +   + A AM+K +Q     GF    VE
Sbjct: 681 RGEQMASYCEEFPSAKDSLHSAANAMIKALQFGVDAGFYCLMVE 715

BLAST of Lag0009492 vs. ExPASy TrEMBL
Match: A0A2N9F1N7 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS8890 PE=4 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 6.1e-34
Identity = 90/284 (31.69%), Postives = 137/284 (48.24%), Query Frame = 0

Query: 5   DRLIWHFEENDPFSVRSRYRLALKLKSQGLPSSSDMTQGRVWWSSLWKLNLPSKVKLFLW 64
           D ++W    N  FS RS Y+L    KS    S+SD ++   +W SLW + +P+K+K+F+W
Sbjct: 117 DSMVWSGTPNGQFSTRSAYQLQAAEKSSLSASTSDQSRNHSFWKSLWGVAVPNKIKVFMW 176

Query: 65  RLARNRLPTKDNLIKRGIDVTNLCVICHSSLEDCLHVFWKCSVVKSMWLGSKFASLYFSV 124
           R  ++ LPTK NL  RG+  +  C +CH   E   H  W C   +++W  S    L++S+
Sbjct: 177 RACKSSLPTKANLFSRGVLSSCTCPVCHDENETIFHTLWDCQYARTVWQNSLLHKLHYSI 236

Query: 125 MGPNFDFVLWAVKEKLCIHDFEIVVVFWWVVW-NLHNAVLWNGSVNERDLFDWASVYLSS 184
              N++ V+  V       D E      W++W N +NA L   S +   L + A+ Y+  
Sbjct: 237 QVSNWNDVVEEVLRTQRQQDIETFFTLAWMIWGNRNNAWLQKPSADAEILGEKAATYVEE 296

Query: 185 FQQAASSNGAVVAVVEDGQSRGTVACVWRPLDVDELKLNIDAPVRPECGTVGGGAVLRNT 244
           + +       V   VED  SR  +   W P     LK+N+         +VG G V+RN+
Sbjct: 297 YNE-------VTKKVED--SRSVLCRKWSPPTGSMLKMNVATTYFNTRKSVGLGVVIRNS 356

Query: 245 RGEVFFSACFTLPNCWNVDFAEAWAMLKGIQTARKMGFRWFSVE 288
           RGE   S C   P+  +   + A AM+K +Q     GF    VE
Sbjct: 357 RGEQMASYCEEFPSAKDSLHSAANAMIKALQFGVDAGFYCLMVE 391

BLAST of Lag0009492 vs. ExPASy TrEMBL
Match: A0A803P3X4 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 2.3e-33
Identity = 91/292 (31.16%), Postives = 146/292 (50.00%), Query Frame = 0

Query: 3    VEDRLIWHFEENDPFSVRSRYRLALKLKSQGLPSSSDMTQGRVWWSSLWKLNLPSKVKLF 62
            +ED+++WH+ ++  +SVRS YR+A  L+ + + S +++T+   WW  LWKL +P KVK F
Sbjct: 1422 IEDKVLWHYSKDGEYSVRSGYRMAAALQVRDVQSDTEVTE--KWWRLLWKLKIPPKVKHF 1481

Query: 63   LWRLARNRLPTKDNLIKRGIDVTNLCVICHSSL-EDCLHVFWKCSVVKSMWLGSKFASLY 122
            +W+LA + LPT   L +R I V   C  C + + ED  H  W C V + +W  + F    
Sbjct: 1482 VWKLAHSWLPTNAVLARRNIPVEPYCNRCFNGVYEDVFHAIWCCRVNREVWKSTGFYGKI 1541

Query: 123  FSVMGPNFDFVLWAVKEKLCIHDFEIVVVFWWVVWNLHNAVLWNGSVNER-DLFDWASVY 182
                  +    L  +   L   +FE  +V  W +W + N+V   G   +   + +W S +
Sbjct: 1542 QRQGSEDVLAFLMRISTILSKEEFEFFLVLSWNLWYVRNSVNHGGHKPQACAILEWCSKF 1601

Query: 183  LSSFQQAASSNGAVVAVVEDGQSRGTVACVWRPLDVDELKLNIDAPVRPECGTVGGGAVL 242
            LS F+++ +          D Q R   A  W P  +D  K+N+DA VR E G     +V+
Sbjct: 1602 LSEFRESNTPK-------RDRQVR--EAARWTPPGIDAFKINVDAGVRTEAGVASISSVV 1661

Query: 243  RNTRGEVFFSACFTLPNCWNVDFAEAWAMLKGIQTARKMGFRWFSVETDSLR 293
            R+  G V  +A   L        AE  A+ +G+Q   +     F+VE+D L+
Sbjct: 1662 RDHAGMVHSAAATVLLQNMTPLHAELQAIARGLQAGIQCNLPAFNVESDCLQ 1702

BLAST of Lag0009492 vs. ExPASy TrEMBL
Match: A0A5B7BI33 (Uncharacterized protein (Fragment) OS=Davidia involucrata OX=16924 GN=Din_037968 PE=4 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 3.0e-33
Identity = 113/371 (30.46%), Postives = 162/371 (43.67%), Query Frame = 0

Query: 5   DRLIWHFEENDPFSVRSRYRLALKL----KSQGLPSSSDMTQGRVWWSSLWKLNLPSKVK 64
           DRLIWHF +N  +SV+S Y LA  L    K+     SS M      W  LW L+LP+K+K
Sbjct: 9   DRLIWHFSKNGQYSVKSGYHLAANLMASRKNGQQAESSGMISVNKSWKILWSLSLPNKIK 68

Query: 65  LFLWRLARNRLPTKDNLIKRGIDVTNLCVICHSSLEDCLHVFWKCSVVKSMWLGSKFASL 124
             +WR A N LPT   L+ R + V  LC +C +  E  LH+F  CS VK +W  +K  S 
Sbjct: 69  NLIWRAAMNILPTGSRLVDRHVPVDPLCRLCENGPETPLHLFLHCSWVKLVWDSAK-GSF 128

Query: 125 YFSVMGPNFDFVLWAVKEKLCIHDFEIVVVFWWVVWNLHNAVLWN--GSVNERDLFDWAS 184
             +++  +F      +  KL     E+  V  W+VW   NA+  +  G    R +FD A 
Sbjct: 129 PDALVHLDFRQFFEYICNKLDSSFIELWCVIIWIVWGHRNAIYHDRQGKDPLRVVFD-AQ 188

Query: 185 VYLSSFQQAASSNGAVVAVVEDGQSRGTVACVWRPLDVDELKLNIDAPVRPECGTVGGGA 244
            YL+ + +A          +    S    + VW P   D  KLN+D    P   + G G 
Sbjct: 189 QYLNEYHEAQHR-----LHIPPSVSSRAGSSVWSPPPEDLFKLNVDGSWVPGSFSGGIGG 248

Query: 245 VLRNTRGEVFFSACFTLPNCWNVDFAEAWAMLKGIQTARKMGFRWFSVETDSL------- 304
           V+R++RG V       L  C + D+AEA AM  G+  A+++G     +E+D L       
Sbjct: 249 VIRDSRGLVIAGFAKPLKWCGSADYAEACAMFFGVAFAKEIGIVDVLIESDCLSLVNSVG 308

Query: 305 --------------------------------RKGNMTAHVMTSLAFTY-ANFVWLEEWP 330
                                           R  N TAH + S A       +W+E  P
Sbjct: 309 CSSPDFSHIGHITDDIRRGMVGLRSFQVRHVRRSANQTAHEIASFARDVDEELLWIENLP 368

BLAST of Lag0009492 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 74.3 bits (181), Expect = 2.0e-13
Identity = 70/300 (23.33%), Postives = 119/300 (39.67%), Query Frame = 0

Query: 5   DRLIWHFEENDPFSVRSRYRLALK-LKSQGLPSSSDMTQGRVWWSSLWKLNLPSKVKLFL 64
           D   W +  +  ++V+S Y +  + +  +  P           +  +WK     K++ FL
Sbjct: 211 DSYTWDYTSSGDYTVKSGYWVLTQIINKRSSPQEVSEPSLNPIYQKIWKSQTSPKIQHFL 270

Query: 65  WRLARNRLPTKDNLIKRGIDVTNLCVICHSSLEDCLHVFWKCSVVKSMW--------LGS 124
           W+   N LP    L  R +   + C+ C S  E   H+ +KC+  +  W        LG 
Sbjct: 271 WKCLSNSLPVAGALAYRHLSKESACIRCPSCKETVNHLLFKCTFARLTWAISSIPIPLGG 330

Query: 125 KFA-SLY---FSVMGPNFDFVLWAVKEKLCIHDFEIVVVFWWVVWNLHNAVLWNG-SVNE 184
           ++A S+Y   + V         W   EK      ++V    W +W   N +++ G   N 
Sbjct: 331 EWADSIYVNLYWVFNLGNGNPQW---EKAS----QLVPWLLWRLWKNRNELVFRGREFNA 390

Query: 185 RDLFDWASVYLSSFQQAASSNGAVVAVVEDGQSRGTVACVWRPLDVDELKLNIDAPVRPE 244
           +++   A   L  ++    +         +  S G     WRP     +K N DA    +
Sbjct: 391 QEVLRRAEDDLEEWRIRTEAESCGTKPQVNRSSCGR----WRPPPHQWVKCNTDATWNRD 450

Query: 245 CGTVGGGAVLRNTRGEVFFSACFTLPNCWNVDFAEAWAMLKGIQTARKMGFRWFSVETDS 291
               G G VLRN +GEV +     LP   +V  AE  AM   + +  +  + +   E+DS
Sbjct: 451 NERCGIGWVLRNEKGEVKWMGARALPKLKSVLEAELEAMRWAVLSLSRFQYNYVIFESDS 499

BLAST of Lag0009492 vs. TAIR 10
Match: AT2G02650.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 66.2 bits (160), Expect = 5.5e-11
Identity = 60/268 (22.39%), Postives = 100/268 (37.31%), Query Frame = 0

Query: 35  PSSSDMTQGRVWWSSLWKLNLPSKVKLFLWRLARNRLPTKDNLIKRGIDVTNLCVICHSS 94
           P S+++ Q      ++WKL++  K+K FLWR     L T   L  R ID   +C  C   
Sbjct: 28  PGSTEVKQ------AIWKLHVAPKIKHFLWRCVTGALATNTRLRSRNIDADPICQRCCIE 87

Query: 95  LEDCLHVFWKCSVVKSMWLGSKFASLYFSVMGPNFDFV-----LWAVKEKLCIHDFEIVV 154
            E   H+ + C   +S+W  +    +  +  GP   F      L  + +    +  +  +
Sbjct: 88  EETIHHIMFNCPYTQSVWRSANI--IIGNQWGPPSSFEDNLNRLIQLSKTQTTNSLDRFL 147

Query: 155 VFW--WVVWNLHNAVLWNGSVNERDL-----FDWASVYLSSFQQAASSNGAVVAVVEDGQ 214
            FW  W +W   N  L+       D         A+ +L++ +   ++N  V       Q
Sbjct: 148 PFWIMWRLWKSRNVFLFQQKCQSPDYEARKGIQDATEWLNANETTENTN--VHVATNPIQ 207

Query: 215 SRGTVACVWRPLDVDELKLNIDAPVRPECGTVGGGAVLRNTRGEVFFSACFTLPNCWNVD 274
           +    +  W P     +K N D+           G  +R   G +       L +     
Sbjct: 208 TSRRDSSQWNPPPEGWVKCNFDSGYTQGSPYTRSGWTIRECNGHIVLCGNAKLQSSTCSL 267

Query: 275 FAEAWAMLKGIQTARKMGFRWFSVETDS 291
            AEA   L  +Q     G R+   E+DS
Sbjct: 268 HAEALGFLHALQVIWAHGLRYVWFESDS 285

BLAST of Lag0009492 vs. TAIR 10
Match: AT3G25270.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 61.6 bits (148), Expect = 1.4e-09
Identity = 59/285 (20.70%), Postives = 101/285 (35.44%), Query Frame = 0

Query: 48  SSLWKLNLPSKVKLFLWRLARNRLPTKDNLIKRGIDVTNLCVICHSSLEDCLHVFWKCSV 107
           + +WKL    K+K FLW+L    L T DNL +R I     C  C    E   H+F+ C  
Sbjct: 16  AKIWKLKTAPKIKHFLWKLLSGALATGDNLKRRHIRNHPQCHRCCQEDETSQHLFFDCFY 75

Query: 108 VKSMWLGSKFASLYFSVMGPNFDFVLWAVKEKLCIHD-----FEIVVVFWWVVWNLHN-- 167
            + +W  S          G   +  +  +    C+ +     F + +   W +W   N  
Sbjct: 76  AQQVWRASGIPHQELRTTGITMETKMELLLSS-CLANRQPQLFNLAIWILWRLWKSRNQL 135

Query: 168 -----AVLWNGSVN--ERDLFDW--ASVYLSSFQQAASSNGAVVAVVEDGQSRGTVACVW 227
                ++ W  ++     D+ +W   + Y+ S  Q   S+          Q        W
Sbjct: 136 VFQQKSISWQNTLQRARNDVQEWEDTNTYVQSLNQQVHSS--------RHQQPTMARTKW 195

Query: 228 RPLDVDELKLNIDAPVRPECGTVGGGAVLRNTRGEVFFSACFTLPNCWNVDFAEAWAMLK 287
           +      +K N D     +      G ++R+  G    S         +   +E  A++ 
Sbjct: 196 QRPPSTWIKYNYDGAFNHQTRNAKAGWLMRDENGVYMGSGQAIGSTTSDSLESEFQALII 255

Query: 288 GIQTARKMGFRWFSVETDSLRKGNMTAHVMTSLAFTYANFVWLEE 317
            +Q A   G+R    E DS         +M +    +  F W+ E
Sbjct: 256 AMQHAWSQGYRKVIFEGDS----KQVEELMNNEKLNFGRFNWIRE 287

BLAST of Lag0009492 vs. TAIR 10
Match: AT1G43730.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 54.7 bits (130), Expect = 1.7e-07
Identity = 30/111 (27.03%), Postives = 54/111 (48.65%), Query Frame = 0

Query: 4   EDRLIWHFEENDPFSVRSRYRLALKLKSQG--LPSSSDMTQGRVWWSSLWKLNLPSKVKL 63
           +D  IW  + + P ++ S  + +L L  Q   +P          W+ ++W  N   K   
Sbjct: 117 DDSFIWKTDLHAPSNIFSTAKTSLALHPQNHIVP----------WYKAVWFKNHVPKHAF 176

Query: 64  FLWRLARNRLPTKDNLIKRGIDVTNLCVICHSSLEDCLHVFWKCSVVKSMW 113
             W +A NRL T+D L   G+ +  +C++C+S  E   H+F++C    ++W
Sbjct: 177 ICWVVAWNRLHTRDRLRSWGLSIPAVCLLCNSHDESRAHLFFECPFCGAVW 217

BLAST of Lag0009492 vs. TAIR 10
Match: AT5G16486.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 45.8 bits (107), Expect = 7.7e-05
Identity = 20/60 (33.33%), Postives = 33/60 (55.00%), Query Frame = 0

Query: 46  WWSSLWKLNLPSKVKLFLWRLARNRLPTKDNLIKRGIDVTNLCVICHSSLEDCLHVFWKC 105
           W  ++W      K     W   R+RLPT+D L+  G+ V +LC++C++  E   H+F+ C
Sbjct: 144 WHKAIWFKGRIPKHAFISWVNIRHRLPTRDKLLSWGLHVPSLCLLCNAFDETRQHLFFDC 203

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022150918.11.2e-4734.20uncharacterized protein LOC111018954 [Momordica charantia][more]
XP_030941688.11.2e-3634.35uncharacterized protein LOC115966628 [Quercus lobata][more]
XP_023904177.12.8e-3332.30uncharacterized protein LOC112015942 [Quercus suber][more]
XP_024956542.14.1e-3231.03uncharacterized protein LOC112498908 [Citrus sinensis][more]
XP_023908235.19.0e-3228.76uncharacterized protein LOC112019924 [Quercus suber][more]
Match NameE-valueIdentityDescription
P0C2F63.6e-1524.61Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1... [more]
Match NameE-valueIdentityDescription
A0A6J1DAR45.7e-4834.20uncharacterized protein LOC111018954 OS=Momordica charantia OX=3673 GN=LOC111018... [more]
A0A2N9IZ426.1e-3431.69Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=F... [more]
A0A2N9F1N76.1e-3431.69Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS8890 PE=4 SV=1[more]
A0A803P3X42.3e-3331.16Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A5B7BI333.0e-3330.46Uncharacterized protein (Fragment) OS=Davidia involucrata OX=16924 GN=Din_037968... [more]
Match NameE-valueIdentityDescription
AT4G29090.12.0e-1323.33Ribonuclease H-like superfamily protein [more]
AT2G02650.15.5e-1122.39Ribonuclease H-like superfamily protein [more]
AT3G25270.11.4e-0920.70Ribonuclease H-like superfamily protein [more]
AT1G43730.11.7e-0727.03RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
AT5G16486.17.7e-0533.33RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026960Reverse transcriptase zinc-binding domainPFAMPF13966zf-RVTcoord: 17..112
e-value: 1.2E-21
score: 77.2
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 230..291
e-value: 5.3E-10
score: 39.2
NoneNo IPR availablePANTHERPTHR46736FAMILY NOT NAMEDcoord: 4..291
NoneNo IPR availablePANTHERPTHR46736:SF6SUBFAMILY NOT NAMEDcoord: 4..291
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 221..290
e-value: 1.59061E-8
score: 50.3904
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 218..291

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0009492.1Lag0009492.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity