Lag0009474 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0009474
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	RNase H domain-containing protein
Location	chr9: 39531513 .. 39532860 (+)
RNA-Seq Expression	Lag0009474
Synteny	Lag0009474

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACATGTCTCCTCACCCAATTTCAGGTTCATGGTTTTTGTCCAAGATTCCCAGTAGGTGAGATTAATTGGAAAATAAAAATGAGAGAGAGAAAAAAAAAAAAAAAAAAAAAACTTCCCTTTAAGATTGAATTATATTATATATTCATCTTTATATGTTTGGTAGGTTTAGATATATATATATATATATATATTTAATTTCTATCTGATTAATTTAGCTAGCTAGATTATTCTAATGCTTTTTAAGAGTCACATCTTAGCTTGTGAAGTTTGTTAAATCTTAATTAGCAATGGACACTTATCATTAATGAATGATATTGATTTTTAACATTAAATTGACCTAGATAGTATTTATGAATTGTGACATGAACTTTGATTACTAATTGTGTAGTTTCTTTGGAAACTATATATTAAAATGATACTTGTCAGATTTACTAATTTCTTTACCAGATATCATATTTCAAGTGGTAAAAGAGGTGTTTATTCACTTATTCTTTACATTAATTTTCACCATTAATGTTATTTATTTAATTAATATATTTCAAGTAGTACTACTAAGCACAAATATGGAAAGTCAACAAGTTGTAATTATCTTATAGATAGATTAGTGCCTACTATATTTCTTGATAGAGAAGGGACTAATATGCCTACAATTATAGTATAGATTTAGCTTTTTTTTTTTCCTTTTTTTCTTTTTTGGAAAGTAGTATAGGCTGTTTAGTAAAAAGTAATATAAGATAATTGATGTGATACCAGTTTATATATACATTAAGCTTGGATCCCTCCTAACTCCCCTCGATGGAAGTTCAATTCAGACGCGTCCTGGAATGCGGAGAAGAAGATCGGAGGGTTGGGGTGGGTAGTTCGTGATTCCGGCGGTTCTTTGGTCTGTGCGGACTTGCAGACATCTAGAGGGGAGTGGTCGATCGATGTTCTTGAGGCCAAAGCCATGTGGGAAGGACTTAAGTCTGTGCTTGTCTTGGTCGGGGATGCGAAAGAAATTGAAGTAGAAGCGGATGCTCTTGATCTCATCCGCTGTTTGAACAATCAAGAGGAAAATTTGACAGAGATCAAGTGTATTGTTGAAGCCATTAACACTTTGGCTCCCAAGCTTGGAGTGCTCTCCTTCAAGCATTGCCCTAGAAGTCAGAATGGGGTGGCTCACTCCATCGCTCGTGCAGGCGCTTTTCATTCTTGTGCTCCTGTTTTTTGTAATTCTGATTCTTTTGTTGGGGATCAGAGGAATCCTTCCACGCTGGAAGACAGTTATTGTTTTTGGTCCTCTGATCCTCCAGAGTGGTTATCCTCCTTGATTTCTAAGGAGTTGGTTGAACTTGATTCTTCTTAA

mRNA sequence

ATGACATGTCTCCTCACCCAATTTCAGGTTCATGGTTTTTGTCCAAGATTCCCAGTAGCTTGGATCCCTCCTAACTCCCCTCGATGGAAGTTCAATTCAGACGCGTCCTGGAATGCGGAGAAGAAGATCGGAGGGTTGGGGTGGGTAGTTCGTGATTCCGGCGGTTCTTTGGTCTGTGCGGACTTGCAGACATCTAGAGGGGAGTGGTCGATCGATGTTCTTGAGGCCAAAGCCATGTGGGAAGGACTTAAGTCTGTGCTTGTCTTGGTCGGGGATGCGAAAGAAATTGAAGTAGAAGCGGATGCTCTTGATCTCATCCGCTGTTTGAACAATCAAGAGGAAAATTTGACAGAGATCAAGTGTATTGTTGAAGCCATTAACACTTTGGCTCCCAAGCTTGGAGTGCTCTCCTTCAAGCATTGCCCTAGAAGTCAGAATGGGGTGGCTCACTCCATCGCTCGTGCAGGCGCTTTTCATTCTTGTGCTCCTGTTTTTTGTAATTCTGATTCTTTTGTTGGGGATCAGAGGAATCCTTCCACGCTGGAAGACAGTTATTGTTTTTGGTCCTCTGATCCTCCAGAGTGGTTATCCTCCTTGATTTCTAAGGAGTTGGTTGAACTTGATTCTTCTTAA

Coding sequence (CDS)

ATGACATGTCTCCTCACCCAATTTCAGGTTCATGGTTTTTGTCCAAGATTCCCAGTAGCTTGGATCCCTCCTAACTCCCCTCGATGGAAGTTCAATTCAGACGCGTCCTGGAATGCGGAGAAGAAGATCGGAGGGTTGGGGTGGGTAGTTCGTGATTCCGGCGGTTCTTTGGTCTGTGCGGACTTGCAGACATCTAGAGGGGAGTGGTCGATCGATGTTCTTGAGGCCAAAGCCATGTGGGAAGGACTTAAGTCTGTGCTTGTCTTGGTCGGGGATGCGAAAGAAATTGAAGTAGAAGCGGATGCTCTTGATCTCATCCGCTGTTTGAACAATCAAGAGGAAAATTTGACAGAGATCAAGTGTATTGTTGAAGCCATTAACACTTTGGCTCCCAAGCTTGGAGTGCTCTCCTTCAAGCATTGCCCTAGAAGTCAGAATGGGGTGGCTCACTCCATCGCTCGTGCAGGCGCTTTTCATTCTTGTGCTCCTGTTTTTTGTAATTCTGATTCTTTTGTTGGGGATCAGAGGAATCCTTCCACGCTGGAAGACAGTTATTGTTTTTGGTCCTCTGATCCTCCAGAGTGGTTATCCTCCTTGATTTCTAAGGAGTTGGTTGAACTTGATTCTTCTTAA

Protein sequence

MTCLLTQFQVHGFCPRFPVAWIPPNSPRWKFNSDASWNAEKKIGGLGWVVRDSGGSLVCADLQTSRGEWSIDVLEAKAMWEGLKSVLVLVGDAKEIEVEADALDLIRCLNNQEENLTEIKCIVEAINTLAPKLGVLSFKHCPRSQNGVAHSIARAGAFHSCAPVFCNSDSFVGDQRNPSTLEDSYCFWSSDPPEWLSSLISKELVELDSS

Homology

[Close]

BLAST of Lag0009474 vs. NCBI nr
Match: XP_022156777.1 (uncharacterized protein LOC111023608 [Momordica charantia])

HSP 1 Score: 99.4 bits (246), Expect = 4.0e-17
Identity = 50/140 (35.71%), Postives = 81/140 (57.86%), Query Frame = 0


Query: 21  WIPPNSPRWKFNSDASWNAEKKIGGLGWVVRDSGGSLVCADLQTSRGEWSIDVLEAKAMW 80
           W PP S  WK N+DA+W A+   GG+GW++RD  G ++ AD +  R E +I  LE  A+ 
Sbjct: 79  WKPPTSNSWKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAIC 138

Query: 81  EGLKSVL------VLVGDAKEIEVEADALDLIRCLNNQEENLTEIKCIVEAINTLAPKLG 140
           EGL+++       +     + I +E+D+L+ I  L+ Q ++ TEI  ++E I  +   + 
Sbjct: 139 EGLRAIRQEHCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMEDMK 198

Query: 141 VLSFKHCPRSQNGVAHSIAR 155
           ++S +H  R  N VAH +AR
Sbjct: 199 IVSMRHISREANKVAHDLAR 218

[Close]

BLAST of Lag0009474 vs. NCBI nr
Match: XP_022143535.1 (uncharacterized protein LOC111013412 [Momordica charantia])

HSP 1 Score: 97.1 bits (240), Expect = 2.0e-16
Identity = 48/134 (35.82%), Postives = 79/134 (58.96%), Query Frame = 0


Query: 21  WIPPNSPRWKFNSDASWNAEKKIGGLGWVVRDSGGSLVCADLQTSRGEWSIDVLEAKAMW 80
           W PP S  WK N++A+W A+   GG+GW++RD  G ++ A  +  R E +I  LE  A+ 
Sbjct: 79  WKPPTSNSWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAIC 138

Query: 81  EGLKSVLVLVGDAKEIEVEADALDLIRCLNNQEENLTEIKCIVEAINTLAPKLGVLSFKH 140
           EGL++  +     + I +E+D+L+ I  L+ Q ++ TEI  ++E I  +   + ++S +H
Sbjct: 139 EGLRA--IRQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMRH 198

Query: 141 CPRSQNGVAHSIAR 155
             R  N VAH +AR
Sbjct: 199 ISREANKVAHGLAR 210

[Close]

BLAST of Lag0009474 vs. NCBI nr
Match: XP_022155262.1 (uncharacterized protein LOC111022403 [Momordica charantia])

HSP 1 Score: 86.7 bits (213), Expect = 2.7e-13
Identity = 46/134 (34.33%), Postives = 74/134 (55.22%), Query Frame = 0


Query: 21  WIPPNSPRWKFNSDASWNAEKKIGGLGWVVRDSGGSLVCADLQTSRGEWSIDVLEAKAMW 80
           W PP    W  N+DASW+     GG+GW++R   G +V A  +      ++ +LEA A+ 
Sbjct: 71  WEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAIL 130

Query: 81  EGLKSVLVLVGDAKEIEVEADALDLIRCLNNQEENLTEIKCIVEAINTLAPKLGVLSFKH 140
           EGL++ L  +G  + + +E D+ ++   LN + E+LT+   +VE I  L     +L+F  
Sbjct: 131 EGLRN-LTNLGVLRPLHIETDSAEVESLLNRKREDLTKTGWVVEEILNLRDSCEILAFAK 190

Query: 141 CPRSQNGVAHSIAR 155
             R  NG AHS+A+
Sbjct: 191 VGRETNGCAHSLAQ 203

[Close]

BLAST of Lag0009474 vs. NCBI nr
Match: XP_022148549.1 (uncharacterized protein LOC111017181 [Momordica charantia])

HSP 1 Score: 86.3 bits (212), Expect = 3.5e-13
Identity = 51/136 (37.50%), Postives = 64/136 (47.06%), Query Frame = 0


Query: 19  VAWIPPNSPRWKFNSDASWNAEKKIGGLGWVVRDSGGSLVCADLQTSRGEWSIDVLEAKA 78
           V W PP    WK N DA+W      GGLGW+VRDS G  + A+                 
Sbjct: 76  VGWTPPAQHLWKLNVDATWMDSLHAGGLGWIVRDSEGRFIMAE----------------- 135

Query: 79  MWEGLKSVLVLVGDAKEIEVEADALDLIRCLNNQEENLTEIKCIVEAINTLAPKLGVLSF 138
             + L   L L    K IE+E+D L+++  +N     LTE+  IVE I      L +  F
Sbjct: 136 CLKALSQSLTLEAGIK-IEMESDCLEVVNIINKSSMVLTEVSLIVEDIWKEMESLPIEGF 193

Query: 139 KHCPRSQNGVAHSIAR 155
           KH P   NGVAH IAR
Sbjct: 196 KHLPMKANGVAHGIAR 193

[Close]

BLAST of Lag0009474 vs. NCBI nr
Match: XP_015388431.1 (uncharacterized protein LOC107178124 [Citrus sinensis])

HSP 1 Score: 85.5 bits (210), Expect = 6.0e-13
Identity = 51/149 (34.23%), Postives = 85/149 (57.05%), Query Frame = 0


Query: 20  AWIPPNSPRWKFNSDASWNAEKKIGGLGWVVRDSGGSLVCADLQTSRGEWSIDVLEAKAM 79
           +W PP +  +K N DA+ N +K+I GLG V+R++ G++V A +  S+    +  +EA AM
Sbjct: 131 SWTPPPAGCYKLNVDAATNRDKQISGLGAVIRNAEGNVVAAAINFSKFFGDVAYVEAAAM 190

Query: 80  WEGLKSVLVLVGDAK--EIEVEADALDLIRCLNNQEENLTEIKCIVEAINTLAPKLGVLS 139
             GL+    + G+A    + VE D+ ++   +NN++ ++TEI  +V AI +L      + 
Sbjct: 191 EFGLQ----VAGNANLPSLIVETDSQEVAGFVNNRQSSMTEIWWVVAAIQSLMKNFNQIK 250

Query: 140 FKHCPRSQNGVAHSIARAGAFHSCAPVFC 167
            +H PRS N +AHS+ +  A   C  V C
Sbjct: 251 VQHIPRSCNTIAHSLTKL-ALEKCENVKC 274

[Close]

BLAST of Lag0009474 vs. ExPASy TrEMBL
Match: A0A6J1DSV1 (uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023608 PE=4 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 1.9e-17
Identity = 50/140 (35.71%), Postives = 81/140 (57.86%), Query Frame = 0


Query: 21  WIPPNSPRWKFNSDASWNAEKKIGGLGWVVRDSGGSLVCADLQTSRGEWSIDVLEAKAMW 80
           W PP S  WK N+DA+W A+   GG+GW++RD  G ++ AD +  R E +I  LE  A+ 
Sbjct: 79  WKPPTSNSWKLNTDAAWRADTNTGGIGWILRDEKGEVIKADCRIIRTERNITYLEVMAIC 138

Query: 81  EGLKSVL------VLVGDAKEIEVEADALDLIRCLNNQEENLTEIKCIVEAINTLAPKLG 140
           EGL+++       +     + I +E+D+L+ I  L+ Q ++ TEI  ++E I  +   + 
Sbjct: 139 EGLRAIRQEHCRPIQQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMEDMK 198

Query: 141 VLSFKHCPRSQNGVAHSIAR 155
           ++S +H  R  N VAH +AR
Sbjct: 199 IVSMRHISREANKVAHDLAR 218

[Close]

BLAST of Lag0009474 vs. ExPASy TrEMBL
Match: A0A6J1CP26 (uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013412 PE=4 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 9.6e-17
Identity = 48/134 (35.82%), Postives = 79/134 (58.96%), Query Frame = 0


Query: 21  WIPPNSPRWKFNSDASWNAEKKIGGLGWVVRDSGGSLVCADLQTSRGEWSIDVLEAKAMW 80
           W PP S  WK N++A+W A+   GG+GW++RD  G ++ A  +  R E +I  LE  A+ 
Sbjct: 79  WKPPTSNSWKLNTNAAWRADTNTGGIGWILRDEKGEVIKASCRIIRAERNITYLEVMAIC 138

Query: 81  EGLKSVLVLVGDAKEIEVEADALDLIRCLNNQEENLTEIKCIVEAINTLAPKLGVLSFKH 140
           EGL++  +     + I +E+D+L+ I  L+ Q ++ TEI  ++E I  +   + ++S +H
Sbjct: 139 EGLRA--IRQEHCRPIHLESDSLEAIHLLHRQCQDQTEIIWLLEEIWQMMKDMEIVSMRH 198

Query: 141 CPRSQNGVAHSIAR 155
             R  N VAH +AR
Sbjct: 199 ISREANKVAHGLAR 210

[Close]

BLAST of Lag0009474 vs. ExPASy TrEMBL
Match: A0A6J1DNV9 (uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022403 PE=4 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 1.3e-13
Identity = 46/134 (34.33%), Postives = 74/134 (55.22%), Query Frame = 0


Query: 21  WIPPNSPRWKFNSDASWNAEKKIGGLGWVVRDSGGSLVCADLQTSRGEWSIDVLEAKAMW 80
           W PP    W  N+DASW+     GG+GW++R   G +V A  +      ++ +LEA A+ 
Sbjct: 71  WEPPPMHIWTLNADASWSDSTHRGGIGWIIRSWDGDIVLAGNRFVEACNNVKLLEASAIL 130

Query: 81  EGLKSVLVLVGDAKEIEVEADALDLIRCLNNQEENLTEIKCIVEAINTLAPKLGVLSFKH 140
           EGL++ L  +G  + + +E D+ ++   LN + E+LT+   +VE I  L     +L+F  
Sbjct: 131 EGLRN-LTNLGVLRPLHIETDSAEVESLLNRKREDLTKTGWVVEEILNLRDSCEILAFAK 190

Query: 141 CPRSQNGVAHSIAR 155
             R  NG AHS+A+
Sbjct: 191 VGRETNGCAHSLAQ 203

[Close]

BLAST of Lag0009474 vs. ExPASy TrEMBL
Match: A0A6J1D4B6 (uncharacterized protein LOC111017181 OS=Momordica charantia OX=3673 GN=LOC111017181 PE=4 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 1.7e-13
Identity = 51/136 (37.50%), Postives = 64/136 (47.06%), Query Frame = 0


Query: 19  VAWIPPNSPRWKFNSDASWNAEKKIGGLGWVVRDSGGSLVCADLQTSRGEWSIDVLEAKA 78
           V W PP    WK N DA+W      GGLGW+VRDS G  + A+                 
Sbjct: 76  VGWTPPAQHLWKLNVDATWMDSLHAGGLGWIVRDSEGRFIMAE----------------- 135

Query: 79  MWEGLKSVLVLVGDAKEIEVEADALDLIRCLNNQEENLTEIKCIVEAINTLAPKLGVLSF 138
             + L   L L    K IE+E+D L+++  +N     LTE+  IVE I      L +  F
Sbjct: 136 CLKALSQSLTLEAGIK-IEMESDCLEVVNIINKSSMVLTEVSLIVEDIWKEMESLPIEGF 193

Query: 139 KHCPRSQNGVAHSIAR 155
           KH P   NGVAH IAR
Sbjct: 196 KHLPMKANGVAHGIAR 193

[Close]

BLAST of Lag0009474 vs. ExPASy TrEMBL
Match: A0A6J1D5W1 (uncharacterized protein LOC111017329 OS=Momordica charantia OX=3673 GN=LOC111017329 PE=4 SV=1)

HSP 1 Score: 80.9 bits (198), Expect = 7.1e-12
Identity = 41/129 (31.78%), Postives = 71/129 (55.04%), Query Frame = 0


Query: 29  WKFNSDASWNAEKKIGGLGWVVRDSGGSLVCADLQTSRGEWSIDVLEAKAMWEGLKSVLV 88
           WK N+DA+W+A + +GGLGW++R+    +  A  QT      I  LE  A+W G+++  V
Sbjct: 13  WKLNTDAAWHANQGVGGLGWIIRNEKREIKKAGGQTITDARDITYLELMAIWLGIEA--V 72

Query: 89  LVGDAKEIEVEADALDLIRCLNNQEENLTEIKCIVEAINTLAPKLGVLSFKHCPRSQNGV 148
           +   +  + +E+++L+ I  +   ++N+TEI  +V+ I     +  +  F+H  R  N V
Sbjct: 73  VSSSSVSLIIESESLEAIHLIQGIQQNVTEILWLVQDIKNFGERARLKRFQHVQRDGNSV 132

Query: 149 AHSIARAGA 158
           A  IA   A
Sbjct: 133 AQEIANRAA 139

[Close]

BLAST of Lag0009474 vs. TAIR 10
Match: AT5G65005.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 57.0 bits (136), Expect = 2.1e-08
Identity = 36/149 (24.16%), Postives = 66/149 (44.30%), Query Frame = 0


Query: 21  WIPPNSPRWKFNSDASWNAEKKIGGLGWVVRDSGGSLVCADLQTSRGEWSIDVLEAKAMW 80
           W PP   + K N DAS +    + GLGW++R+S G+++   +   +G  + +  E   + 
Sbjct: 105 WSPPGRDKLKCNYDASHHERNTVSGLGWILRNSQGTVIECGMGKFQGRMTTEEAECSTLI 164

Query: 81  EGLKSVLVLVGDAKEIEVEADALDLIRCLNNQEENLTEIKCIVEAINTLAPKLGVLSFKH 140
             +++        K++  E D   + R +N +  N   ++  ++ I +  P    + F  
Sbjct: 165 WAIQASYGF--GHKKVIFEGDNQTITRMINTKSSN-PRLQHFLDTIQSWIPSFESIEFSF 224

Query: 141 CPRSQNGVAHSIARA--------GAFHSC 162
             R QNG A  +A+           FHSC
Sbjct: 225 KHREQNGCADFLAKQAIKENTQWSLFHSC 250

[Close]

BLAST of Lag0009474 vs. TAIR 10
Match: AT2G34320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 49.7 bits (117), Expect = 3.4e-06
Identity = 44/139 (31.65%), Postives = 68/139 (48.92%), Query Frame = 0


Query: 19  VAWIPPNSPRWKFNSDASWNAEKKIGGLGWVVR-DSGGSLVCADLQTSRGEWSIDVLEAK 78
           V W  P     K N+DA+W  E    G+GW++R +SGG L        R +   +VLEA+
Sbjct: 135 VQWKAPPYQWVKCNTDATWQLENPRCGIGWILRNESGGVLWMGARALPRTK---NVLEAE 194

Query: 79  AMWEGLKSVLVLVG--DAKEIEVEADALDLIRCLNNQEENLTEIKCIVEAINTLAPKLGV 138
              E L+  ++ +   + K I  E+DA  L+  LN+ ++    ++  +E I  L      
Sbjct: 195 L--EALRWAVLTMSRFNYKRIIFESDAQALVNLLNS-DDFWPTLQPALEDIQQLLHHFEE 254

Query: 139 LSFKHCPRSQNGVAHSIAR 155
           + F+  PR  N VA  IAR
Sbjct: 255 VKFEFTPRGGNKVADRIAR 267

[Close]

BLAST of Lag0009474 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 49.7 bits (117), Expect = 3.4e-06
Identity = 37/134 (27.61%), Postives = 62/134 (46.27%), Query Frame = 0


Query: 21  WIPPNSPRWKFNSDASWNAEKKIGGLGWVVRDSGGSLVCADLQTSRGEWSIDVLEAKAMW 80
           W PP     K N+DA+WN + +  G+GWV+R+  G +     +      S+   E +AM 
Sbjct: 420 WRPPPHQWVKCNTDATWNRDNERCGIGWVLRNEKGEVKWMGARALPKLKSVLEAELEAMR 479

Query: 81  EGLKSVLVLVGDAKEIEVEADALDLIRCLNNQEENLTEIKCIVEAINTLAPKLGVLSFKH 140
             + S+         +  E+D+  LI  LNN +E    +K  ++ +  L  +   + F  
Sbjct: 480 WAVLSLSRF--QYNYVIFESDSQVLIEILNN-DEIWPSLKPTIQDLQRLLSQFTEVKFVF 539

Query: 141 CPRSQNGVAHSIAR 155
            PR  N +A  +AR
Sbjct: 540 IPREGNTLAERVAR 550

[Close]

BLAST of Lag0009474 vs. TAIR 10
Match: AT1G52990.1 (thioredoxin family protein )

HSP 1 Score: 43.1 bits (100), Expect = 3.2e-04
Identity = 36/147 (24.49%), Postives = 66/147 (44.90%), Query Frame = 0


Query: 26  SPRWKFNSDASWNAEKKIGGLGWVVRDSGGSLVCADLQTSRGEWSIDVLEAKAMWEGLKS 85
           S R K N DAS +    + GLGW++R+S G+++   +   +G  + +  E  A+   +++
Sbjct: 45  SCRVKCNYDASHHEGDVVSGLGWLIRNSQGTVLECGMGKFQGRMTPEEAECSALIWAIQA 104

Query: 86  VLVLVGDAKEIEVEADALDLIRCLNNQEENLTEIKCIVEAINTLAPKLGVLSFKHCPRSQ 145
                G  K I  E D  ++ R +N + +N   +K  ++ I +  P      F    R Q
Sbjct: 105 TSAF-GYTKVI-FEGDNSNVNRLINTKSDN-PRLKHYLDTIKSWIPSFTSTEFIFTHREQ 164

Query: 146 NGVAHSIARAG-------AFHSCAPVF 166
           N  A ++ +         +  +C P F
Sbjct: 165 NQCADTLVKKAIKSSTQWSLFNCCPHF 188

[Close]

BLAST of Lag0009474 vs. TAIR 10
Match: AT3G23320.1 (Polynucleotidyl transferase, ribonuclease H-like superfamily protein )

HSP 1 Score: 41.6 bits (96), Expect = 9.2e-04
Identity = 32/134 (23.88%), Postives = 59/134 (44.03%), Query Frame = 0


Query: 21  WIPPNSPRWKFNSDASWNAEKKIGGLGWVVRDSGGSLVCADLQTSRGEWSIDVLEAKAMW 80
           W  P +   K N D S +A ++  GL W++R+S G+ +       +G  +I   E  A+ 
Sbjct: 30  WQKPGAEWVKCNYDVSNHAGRQDSGLRWIIRNSQGTCLDCGCGKFQGRQTIKEAECTALI 89

Query: 81  EGLKSVLVLVGDAKEIEVEADALDLIRCLNNQEENLTEIKCIVEAINTLAPKLGVLSFKH 140
             ++    L    + +E E D + + R + N+E N   ++  +E I   +     + F  
Sbjct: 90  WAIQCAWDL--GYRRVEFEGDNITVNRLIRNKETN-PRLRYYLEFIQQWSKAFTTVKFTF 149

Query: 141 CPRSQNGVAHSIAR 155
             R QN     +A+
Sbjct: 150 RHREQNVCVDVLAK 160

The following BLAST results are available for this feature:

BLAST of Lag0009474 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022156777.1	4.0e-17	35.71	uncharacterized protein LOC111023608 [Momordica charantia]	[more]
XP_022143535.1	2.0e-16	35.82	uncharacterized protein LOC111013412 [Momordica charantia]	[more]
XP_022155262.1	2.7e-13	34.33	uncharacterized protein LOC111022403 [Momordica charantia]	[more]
XP_022148549.1	3.5e-13	37.50	uncharacterized protein LOC111017181 [Momordica charantia]	[more]
XP_015388431.1	6.0e-13	34.23	uncharacterized protein LOC107178124 [Citrus sinensis]	[more]

BLAST of Lag0009474 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Lag0009474 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DSV1	1.9e-17	35.71	uncharacterized protein LOC111023608 OS=Momordica charantia OX=3673 GN=LOC111023...	[more]
A0A6J1CP26	9.6e-17	35.82	uncharacterized protein LOC111013412 OS=Momordica charantia OX=3673 GN=LOC111013...	[more]
A0A6J1DNV9	1.3e-13	34.33	uncharacterized protein LOC111022403 OS=Momordica charantia OX=3673 GN=LOC111022...	[more]
A0A6J1D4B6	1.7e-13	37.50	uncharacterized protein LOC111017181 OS=Momordica charantia OX=3673 GN=LOC111017...	[more]
A0A6J1D5W1	7.1e-12	31.78	uncharacterized protein LOC111017329 OS=Momordica charantia OX=3673 GN=LOC111017...	[more]

BLAST of Lag0009474 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G65005.1	2.1e-08	24.16	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT2G34320.1	3.4e-06	31.65	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]
AT4G29090.1	3.4e-06	27.61	Ribonuclease H-like superfamily protein	[more]
AT1G52990.1	3.2e-04	24.49	thioredoxin family protein	[more]
AT3G23320.1	9.2e-04	23.88	Polynucleotidyl transferase, ribonuclease H-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 32..155 e-value: 1.0E-19 score: 70.5
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 28..161 e-value: 1.6E-12 score: 49.5
None	No IPR available	PANTHER	PTHR47723	OS05G0353850 PROTEIN	coord: 18..159
IPR044730	Ribonuclease H-like domain, plant type	CDD	cd06222	RNase_H_like	coord: 32..154 e-value: 1.02028E-17 score: 73.8876
IPR012337	Ribonuclease H-like superfamily	SUPERFAMILY	53098	Ribonuclease H-like	coord: 28..158

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0009474.1	Lag0009474.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity