Lag0009403 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0009403
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionReverse transcriptase domain-containing protein
Locationchr9: 38950393 .. 38951118 (-)
RNA-Seq ExpressionLag0009403
SyntenyLag0009403
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGTGCCGATTGTTTTGAGAATTTGTTGTTTTGGGCTGGTGGCTGTGGAATAGGAGGAATAAGAGGGTTGTTGGTGGAGGGAATTTGGGAGGGGGATGGAAATGGGTGGTTGTGGGTCTCTGAGTATTTAGACCATTTTAGGGCTTTCAATGGGAGGAGAGTGTGTGGGGGTCTGGTTCGAAGGGAAGGGATACGGTGGATGCCACCAAATTCAGAGAATTATAAGTTTAATACAGATGCAGCAGTATGCAAAGCAATGAAGTCCAGCAGTTTGGCAGCAATTATTCGAGACGCCGAAGGAAGAGTTATGCTTACATTGATGAAATTAGTCTCTTATGTGCAAGATGTGGATGCTCTTGAGGCTATGGTGATCCGTGACAGCCTGCTTGCTGCTAAGGAAGCTGGCCTCCTGAGATTGGAGGTGGAATTGGACTCAGCTCGCGTCGTGGCTTTGATTCGGTCCGAAAAGAGGGATTGTTCTGAAGTGGGCGTCCTCGTGCAAGAAATTATCCAGATCTCGAAGGGTCTGCAGTTCTGTTCCGTGAGTTGGTGCCTAAGGGAAGCAAATCTGGTGGCGCATACGGCGGCGCGTCAGGTCGTGGAGCTGGGTATTCAAGGCGTCTGGTTGGAGGAGACGCCGATGTCGTTGGAGGAGGTCTATTGCAATGAAATTTTGGACAACCAAGGAGAGAGGTTGTGTTTCTTGTTTGAGGGTTCTGTTTAG

mRNA sequence

ATGAGTGCCGATTGTTTTGAGAATTTGTTGTTTTGGGCTGGTGGCTGTGGAATAGGAGGAATAAGAGGGTTGTTGGTGGAGGGAATTTGGGAGGGGGATGGAAATGGGTGGTTGTGGGTCTCTGAGTATTTAGACCATTTTAGGGCTTTCAATGGGAGGAGAGTGTGTGGGGGTCTGGTTCGAAGGGAAGGGATACGGTGGATGCCACCAAATTCAGAGAATTATAAGTTTAATACAGATGCAGCAGTATGCAAAGCAATGAAGTCCAGCAGTTTGGCAGCAATTATTCGAGACGCCGAAGGAAGAGTTATGCTTACATTGATGAAATTAGTCTCTTATGTGCAAGATGTGGATGCTCTTGAGGCTATGGTGATCCGTGACAGCCTGCTTGCTGCTAAGGAAGCTGGCCTCCTGAGATTGGAGGTGGAATTGGACTCAGCTCGCGTCGTGGCTTTGATTCGGTCCGAAAAGAGGGATTGTTCTGAAGTGGGCGTCCTCGTGCAAGAAATTATCCAGATCTCGAAGGGTCTGCAGTTCTGTTCCGTGAGTTGGTGCCTAAGGGAAGCAAATCTGGTGGCGCATACGGCGGCGCGTCAGGTCGTGGAGCTGGGTATTCAAGGCGTCTGGTTGGAGGAGACGCCGATGTCGTTGGAGGAGGTCTATTGCAATGAAATTTTGGACAACCAAGGAGAGAGGTTGTGTTTCTTGTTTGAGGGTTCTGTTTAG

Coding sequence (CDS)

ATGAGTGCCGATTGTTTTGAGAATTTGTTGTTTTGGGCTGGTGGCTGTGGAATAGGAGGAATAAGAGGGTTGTTGGTGGAGGGAATTTGGGAGGGGGATGGAAATGGGTGGTTGTGGGTCTCTGAGTATTTAGACCATTTTAGGGCTTTCAATGGGAGGAGAGTGTGTGGGGGTCTGGTTCGAAGGGAAGGGATACGGTGGATGCCACCAAATTCAGAGAATTATAAGTTTAATACAGATGCAGCAGTATGCAAAGCAATGAAGTCCAGCAGTTTGGCAGCAATTATTCGAGACGCCGAAGGAAGAGTTATGCTTACATTGATGAAATTAGTCTCTTATGTGCAAGATGTGGATGCTCTTGAGGCTATGGTGATCCGTGACAGCCTGCTTGCTGCTAAGGAAGCTGGCCTCCTGAGATTGGAGGTGGAATTGGACTCAGCTCGCGTCGTGGCTTTGATTCGGTCCGAAAAGAGGGATTGTTCTGAAGTGGGCGTCCTCGTGCAAGAAATTATCCAGATCTCGAAGGGTCTGCAGTTCTGTTCCGTGAGTTGGTGCCTAAGGGAAGCAAATCTGGTGGCGCATACGGCGGCGCGTCAGGTCGTGGAGCTGGGTATTCAAGGCGTCTGGTTGGAGGAGACGCCGATGTCGTTGGAGGAGGTCTATTGCAATGAAATTTTGGACAACCAAGGAGAGAGGTTGTGTTTCTTGTTTGAGGGTTCTGTTTAG

Protein sequence

MSADCFENLLFWAGGCGIGGIRGLLVEGIWEGDGNGWLWVSEYLDHFRAFNGRRVCGGLVRREGIRWMPPNSENYKFNTDAAVCKAMKSSSLAAIIRDAEGRVMLTLMKLVSYVQDVDALEAMVIRDSLLAAKEAGLLRLEVELDSARVVALIRSEKRDCSEVGVLVQEIIQISKGLQFCSVSWCLREANLVAHTAARQVVELGIQGVWLEETPMSLEEVYCNEILDNQGERLCFLFEGSV
Homology
BLAST of Lag0009403 vs. NCBI nr
Match: XP_023880084.1 (uncharacterized protein LOC111992454 [Quercus suber])

HSP 1 Score: 96.7 bits (239), Expect = 3.0e-16
Identity = 57/160 (35.62%), Postives = 88/160 (55.00%), Query Frame = 0

Query: 67  WMPPNSENYKFNTDAAVCKAMKSSSLAAIIRDAEGRVMLTLMKLVSYVQDVDALEAMVIR 126
           W PP+S++YK N DA +   +  S + AIIR+  G VM  L     +VQD +  E +  R
Sbjct: 11  WQPPHSDHYKLNFDAVIYTDLGCSGVGAIIRNERGEVMAALSAKGPFVQDSEEAETLACR 70

Query: 127 DSLLAAKEAGLLRLEVELDSARVVALIRSEKRDCSEVGVLVQEIIQISKGLQFCSVSWCL 186
            +L  A + G+L L +E D+A V+  + S   D S +G ++++I  +  GL++ SVS   
Sbjct: 71  KALEFAIDVGILDLIIEGDNASVMRAVASNFMDGSRLGNVLEDIHCLMSGLRWSSVSCVK 130

Query: 187 REANLVAHTAARQVVELGIQGVWLEETPMSLEEVYCNEIL 227
           R AN VAH  AR    +  + VW+E++P    E   N+ L
Sbjct: 131 RSANTVAHCLARFARNVSNELVWIEDSPQPAREALYNDSL 170

BLAST of Lag0009403 vs. NCBI nr
Match: XP_030963892.1 (uncharacterized protein LOC115985062 [Quercus lobata])

HSP 1 Score: 93.6 bits (231), Expect = 2.5e-15
Identity = 55/166 (33.13%), Postives = 91/166 (54.82%), Query Frame = 0

Query: 61  RREGIRWMPPNSENYKFNTDAAVCKAMKSSSLAAIIRDAEGRVMLTLMKLVSYVQDVDAL 120
           +R+   W PP+S++YK N DAA+   +  S + AIIR+  G VM  L     +VQD +  
Sbjct: 30  QRQSSGWQPPHSDHYKLNFDAAIYTDLGCSGVGAIIRNERGEVMAALSAKGPFVQDSEEA 89

Query: 121 EAMVIRDSLLAAKEAGLLRLEVELDSARVVALIRSEKRDCSEVGVLVQEIIQISKGLQFC 180
           E +  R +L  A + G+  L +E D+  V+  + S   D S +G ++++I  + + L++ 
Sbjct: 90  ETLACRKALEFAIDVGISDLIIEGDNVSVMRAVASNCMDGSRLGNILEDIHWLVRSLRWS 149

Query: 181 SVSWCLREANLVAHTAARQVVELGIQGVWLEETPMSLEEVYCNEIL 227
           SVS   R AN VAH+ +R    +  + VW+E++P    E   N+ L
Sbjct: 150 SVSCVKRSANTVAHSLSRFARNVPDELVWIEDSPQPAREALYNDSL 195

BLAST of Lag0009403 vs. NCBI nr
Match: KAF7819670.1 (uncharacterized protein G2W53_025125 [Senna tora])

HSP 1 Score: 93.2 bits (230), Expect = 3.3e-15
Identity = 55/164 (33.54%), Postives = 88/164 (53.66%), Query Frame = 0

Query: 66  RWMPPNSENYKFNTDAAVCKAMKSSSLAAIIRDAEGRVMLTLMKLVSYVQDVDALEAMVI 125
           +W PP+    K N DA++ + M  S +  IIR+  GR ++ + K       VD LEA+ +
Sbjct: 84  KWQPPSWRFLKLNVDASMREGM-PSRVGCIIRNHTGRCLVAMTKRFEVATTVDTLEALAV 143

Query: 126 RDSLLAAKEAGLLRLEVELDSARVVALIRSEKRDCSEVGVLVQEIIQISKGLQFCSVSWC 185
            + L+ AK   +L +EVE DSA+V  L+  +  D S +G+++++I+++S    F S  W 
Sbjct: 144 YEGLMLAKSMSILNIEVEGDSAKVFKLLNKKGTDQSYLGIIIEDILELSSFFNFVSFKWI 203

Query: 186 LREANLVAHTAARQVVELGIQG----VWLEETPMSLEEVYCNEI 226
            RE N +AH  A   VE         VWLE+ P+    + CN +
Sbjct: 204 RRETNKLAHILAHDHVEFMDDNLSTIVWLEDYPL----LICNAL 242

BLAST of Lag0009403 vs. NCBI nr
Match: XP_023876296.1 (uncharacterized protein LOC111988743 [Quercus suber])

HSP 1 Score: 92.8 bits (229), Expect = 4.3e-15
Identity = 56/160 (35.00%), Postives = 86/160 (53.75%), Query Frame = 0

Query: 67  WMPPNSENYKFNTDAAVCKAMKSSSLAAIIRDAEGRVMLTLMKLVSYVQDVDALEAMVIR 126
           W+ P    YK N DAA+ K +  S + AIIR+ +G VM  +      V+D +  E +  R
Sbjct: 234 WVAPTDPVYKLNFDAAIFKELNCSGIGAIIRNGKGEVMAAMSAKGPLVEDSEEAETLACR 293

Query: 127 DSLLAAKEAGLLRLEVELDSARVVALIRSEKRDCSEVGVLVQEIIQISKGLQFCSVSWCL 186
            ++  A +AG   L +E D+A V+A +     D S +G +VQ++  ++ GL++ S S   
Sbjct: 294 KAVEFAIDAGFSELVIEGDNAAVMASLSHGGPDLSRLGHVVQDVQWLATGLRWVSFSHVK 353

Query: 187 REANLVAHTAARQVVELGIQGVWLEETPMSLEEVYCNEIL 227
           R AN VAH  AR    +    +WLEETP  + E   N++L
Sbjct: 354 RCANTVAHGLARHAKNVMEDMIWLEETPPPVVEALSNDLL 393

BLAST of Lag0009403 vs. NCBI nr
Match: XP_023885847.1 (keratin, type I cytoskeletal 13-like [Quercus suber])

HSP 1 Score: 92.8 bits (229), Expect = 4.3e-15
Identity = 55/173 (31.79%), Postives = 91/173 (52.60%), Query Frame = 0

Query: 42  EYLDHFRAFNGRRVCGGLVRREGIRWMPPNSENYKFNTDAAVCKAMKSSSLAAIIRDAEG 101
           +YLD ++  + +     ++    I W PP +  YK N DAA+   +  S   AIIR+ EG
Sbjct: 117 DYLDEYKKSHVQLAATPVMAPARISWKPPPTSTYKLNFDAAIFADLNCSGFGAIIRNEEG 176

Query: 102 RVMLTLMKLVSYVQDVDALEAMVIRDSLLAAKEAGLLRLEVELDSARVVALIRSEKRDCS 161
            VM  +        +    EA+  R ++  A EAG  RL +E D+A V+  I S   + S
Sbjct: 177 EVMAGMSVKGPLSLNSAEAEALACRKAVQFALEAGFSRLVIEGDNALVMNAISSSAENNS 236

Query: 162 EVGVLVQEIIQISKGLQFCSVSWCLREANLVAHTAARQVVELGIQGVWLEETP 215
            +G + ++I  + +GLQ+ S+S   R+ N+VAH+ AR    +  +  W+E++P
Sbjct: 237 LLGHIFEDIQHLIRGLQYVSISCIKRDGNMVAHSLARHARTISDEMYWMEDSP 289

BLAST of Lag0009403 vs. ExPASy TrEMBL
Match: A0A2N9HRK6 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS42141 PE=3 SV=1)

HSP 1 Score: 97.4 bits (241), Expect = 8.4e-17
Identity = 54/182 (29.67%), Postives = 93/182 (51.10%), Query Frame = 0

Query: 43   YLDHFRAFNGRRVCGGLVRREGIRWMPPNSENYKFNTDAAVCKAMKSSSLAAIIRDAEGR 102
            YLD F +          +  +  +W PP+ +N+K N D A+ K    + +  I+R+ EG+
Sbjct: 1687 YLDEFESNAKTSPHTEPIPAQTTKWQPPSCQNFKANYDGAIFKETNEAGIGVIVRNREGK 1746

Query: 103  VMLTLMKLVSYVQDVDALEAMVIRDSLLAAKEAGLLRLEVELDSARVVALIRSEKRDCSE 162
            VM +L++ V Y Q V+++EA   R ++    E GL   E E DS  VVA + S   + + 
Sbjct: 1747 VMASLVQKVRYPQSVESIEAWAARRAVSFVAEIGLSEAEFEGDSKTVVAALNSPYPNLAL 1806

Query: 163  VGVLVQEIIQISKGLQFCSVSWCLREANLVAHTAARQVVELGIQGVWLEETPMSLEEVYC 222
             G L++++  ++ GL     +   R+ N +AH  AR  +      VW+E+ P   + +Y 
Sbjct: 1807 YGHLIEDVKLLTNGLVSSCFTHVKRQGNAMAHALARMAINTANTEVWMEDVPPQTKRLYL 1866

Query: 223  NE 225
            +E
Sbjct: 1867 SE 1868

BLAST of Lag0009403 vs. ExPASy TrEMBL
Match: A0A2N9FJ03 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS18678 PE=4 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 2.1e-15
Identity = 50/182 (27.47%), Postives = 92/182 (50.55%), Query Frame = 0

Query: 43  YLDHFRAFNGRRVCGGLVRREGIRWMPPNSENYKFNTDAAVCKAMKSSSLAAIIRDAEGR 102
           YL+ + + N +          GI+W PP    +K N D AV +    + +  ++RD EG+
Sbjct: 814 YLEEYASCNEKPPPKESAPELGIKWQPPRHLGFKANYDGAVFQESNEAGIGVVVRDREGK 873

Query: 103 VMLTLMKLVSYVQDVDALEAMVIRDSLLAAKEAGLLRLEVELDSARVVALIRSEKRDCSE 162
           VM +L++ V + Q V+ +EA   + ++    E G+   E E DS  +VA + +     + 
Sbjct: 874 VMASLVQKVRHPQSVECIEAWAAKRAVKFVTEIGITEAEFEGDSTTIVAALNNHSPVLTP 933

Query: 163 VGVLVQEIIQISKGLQFCSVSWCLREANLVAHTAARQVVELGIQGVWLEETPMSLEEVYC 222
            G L+ +   ++ GL+ CS +   R+ N +AH  AR  +      VW+E+ P   +++Y 
Sbjct: 934 YGHLITDAKILANGLKSCSFTHVKRQGNGLAHAIARMALHSNNLEVWMEDVPPPTKQMYL 993

Query: 223 NE 225
           ++
Sbjct: 994 SD 995

BLAST of Lag0009403 vs. ExPASy TrEMBL
Match: A0A2N9J7Z2 (Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS61378 PE=4 SV=1)

HSP 1 Score: 92.0 bits (227), Expect = 3.5e-15
Identity = 47/161 (29.19%), Postives = 86/161 (53.42%), Query Frame = 0

Query: 64   GIRWMPPNSENYKFNTDAAVCKAMKSSSLAAIIRDAEGRVMLTLMKLVSYVQDVDALEAM 123
            GI+W PP    +K N D AV +    + +  ++RD EG+VM +L++ V + Q V+ +EA 
Sbjct: 1070 GIKWQPPRHLGFKANYDGAVFQESNEAGIGVVVRDREGKVMASLVQKVHHPQSVECIEAW 1129

Query: 124  VIRDSLLAAKEAGLLRLEVELDSARVVALIRSEKRDCSEVGVLVQEIIQISKGLQFCSVS 183
              + ++    E G+  +E E DS  +VA + +     +  G L+ +   ++ GL+ CS +
Sbjct: 1130 AAKKAVKFVTEIGITEVEFEGDSTTIVAALNNHSPVLTPYGHLITDAKILANGLKSCSFT 1189

Query: 184  WCLREANLVAHTAARQVVELGIQGVWLEETPMSLEEVYCNE 225
               R+ N +AH  AR  +      VW+E+ P   +++Y ++
Sbjct: 1190 HVKRQGNGLAHAIARMALHSNNLEVWMEDVPPPTKQMYLSD 1230

BLAST of Lag0009403 vs. ExPASy TrEMBL
Match: A0A6J1C467 (uncharacterized protein LOC111007775 OS=Momordica charantia OX=3673 GN=LOC111007775 PE=4 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 4.6e-15
Identity = 67/198 (33.84%), Postives = 102/198 (51.52%), Query Frame = 0

Query: 33  DGNGWL------WVSEYLDHFRAFNGRRVCGGLVRREGIR---WMPPNSENYKFNTDAAV 92
           +G+G+L      WV++YL  +++         L+   G R   W PP +  +K N DAA 
Sbjct: 30  NGHGFLLVNMVDWVADYLQVYQSAQEGPSLPLLLDGRGGRVSAWCPPLAPFFKVNVDAAF 89

Query: 93  CKAMKSSSLAAIIRDAEGRVMLTLMKLVSYVQDVDALEAMVIRDSLLAAKEAGLLRLEVE 152
            K   S+ L  +IRD+   V+L+ M  +++V DV   E +  ++ +  A EAGL+  ++E
Sbjct: 90  KKGNFSAGLDILIRDSTVNVLLSAMCFITHVSDVTLAECLAAQEGVCLAIEAGLIPFQIE 149

Query: 153 LDSARVVALIRSEKRDCSEVGVLVQEIIQISKGLQF-CSVSWCLREANLVAHTAARQVVE 212
            DS++V  L+R++  D SE+GVL   I  I   L      S+  RE N  AHT AR  + 
Sbjct: 150 TDSSQVFNLLRTDCEDESEIGVLASSIRHIVSSLHIGGGFSFVNREGNSGAHTLARMGMV 209

Query: 213 LGIQGVWLEETPMSLEEV 221
                VW+EE    L EV
Sbjct: 210 SESFHVWVEEWLSDLSEV 227

BLAST of Lag0009403 vs. ExPASy TrEMBL
Match: A0A2N9EZZ2 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS12168 PE=4 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 4.6e-15
Identity = 51/156 (32.69%), Postives = 82/156 (52.56%), Query Frame = 0

Query: 66  RWMPPNSENYKFNTDAAVCKAMKSSSLAAIIRDAEGRVMLTLMKLVSYVQDVDALEAMVI 125
           RWMPP    YK N D AV K    + +  I+RD++G VM +L + V +   V ++EA  +
Sbjct: 310 RWMPPKMLRYKINYDGAVFKETNEAGIGVIVRDSQGLVMASLTQKVLFPLSVPSIEAWAV 369

Query: 126 RDSLLAAKEAGLLRLEVELDSARVVALIRSEKRDCSEVGVLVQEIIQISKGLQFCSVSWC 185
           + S+  A E G+   E E DS  +V  + ++    +  G+L+ +  +++  LQ  S S  
Sbjct: 370 KRSIQFALEIGITEAEFEGDSQTIVNALNAQHPSLAPFGLLLADAKELASKLQNFSFSHV 429

Query: 186 LREANLVAHTAARQVVELGIQGVWLEETPMSLEEVY 222
            RE N +AH  AR+        +W+E  P  LE++Y
Sbjct: 430 KREGNRLAHALARKAHSCNSLEIWMESIPPDLEQLY 465

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023880084.13.0e-1635.63uncharacterized protein LOC111992454 [Quercus suber][more]
XP_030963892.12.5e-1533.13uncharacterized protein LOC115985062 [Quercus lobata][more]
KAF7819670.13.3e-1533.54uncharacterized protein G2W53_025125 [Senna tora][more]
XP_023876296.14.3e-1535.00uncharacterized protein LOC111988743 [Quercus suber][more]
XP_023885847.14.3e-1531.79keratin, type I cytoskeletal 13-like [Quercus suber][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A2N9HRK68.4e-1729.67Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS42141 PE=3 SV=1[more]
A0A2N9FJ032.1e-1527.47Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=F... [more]
A0A2N9J7Z23.5e-1529.19Reverse transcriptase domain-containing protein OS=Fagus sylvatica OX=28930 GN=F... [more]
A0A6J1C4674.6e-1533.84uncharacterized protein LOC111007775 OS=Momordica charantia OX=3673 GN=LOC111007... [more]
A0A2N9EZZ24.6e-1532.69Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS12168 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 73..202
e-value: 2.5E-7
score: 32.8
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 78..199
e-value: 8.7E-20
score: 70.8
NoneNo IPR availablePANTHERPTHR47723OS05G0353850 PROTEINcoord: 64..216
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 78..198
e-value: 1.85036E-13
score: 63.102

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0009403.1Lag0009403.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity