Lag0009398 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0009398
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionReverse transcriptase domain-containing protein
Locationchr9: 38851677 .. 38852168 (-)
RNA-Seq ExpressionLag0009398
SyntenyLag0009398
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGAAGTATGACCTTCAGCGATAATTGGATTGAAAGAATAATGAGTTGCATATCCACTGTGAGCTACTCCATCATGATTAATGGCTCACCCCATGACTTTTTCAAGCCTAGTAGGGGTCTAAGACAAGGAGATCCGCTTTCTCCTTATCTCTTCCTGCGTCACGCGGAAGGCTTCTCAGCGCTCCTAAAAAGGGAGGAATCTCTATCTAATATTGTTGGTTTCCAGATTAATAAATATTGCCCACTGTTGACTCACCTTTTCTTTGTAGATGACAGCTTGATATTTGTTAAATCTAAAGGAAAGGACTTGCACACTTTCAAAAGAATCCTAAAGTGGTACGAGGAAGCTTCGGGCCAAACTATCAATTTGGAGAATTCGTCCTTCATGGCTAGCAAGAATCTAAGTAAGGAAGCTAGGAATGAGTGCGAGAGAATCCTGAGGATAGAGAAAACCGATTCGCTAGGCCACTATTTGGGAATGTCGACTTAA

mRNA sequence

ATGAGAAGTATGACCTTCAGCGATAATTGGATTGAAAGAATAATGAGTTGCATATCCACTGTGAGCTACTCCATCATGATTAATGGCTCACCCCATGACTTTTTCAAGCCTAGTAGGGGTCTAAGACAAGGAGATCCGCTTTCTCCTTATCTCTTCCTGCGTCACGCGGAAGGCTTCTCAGCGCTCCTAAAAAGGGAGGAATCTCTATCTAATATTGTTGGTTTCCAGATTAATAAATATTGCCCACTGTTGACTCACCTTTTCTTTGTAGATGACAGCTTGATATTTGTTAAATCTAAAGGAAAGGACTTGCACACTTTCAAAAGAATCCTAAAGTGGTACGAGGAAGCTTCGGGCCAAACTATCAATTTGGAGAATTCGTCCTTCATGGCTAGCAAGAATCTAAGTAAGGAAGCTAGGAATGAGTGCGAGAGAATCCTGAGGATAGAGAAAACCGATTCGCTAGGCCACTATTTGGGAATGTCGACTTAA

Coding sequence (CDS)

ATGAGAAGTATGACCTTCAGCGATAATTGGATTGAAAGAATAATGAGTTGCATATCCACTGTGAGCTACTCCATCATGATTAATGGCTCACCCCATGACTTTTTCAAGCCTAGTAGGGGTCTAAGACAAGGAGATCCGCTTTCTCCTTATCTCTTCCTGCGTCACGCGGAAGGCTTCTCAGCGCTCCTAAAAAGGGAGGAATCTCTATCTAATATTGTTGGTTTCCAGATTAATAAATATTGCCCACTGTTGACTCACCTTTTCTTTGTAGATGACAGCTTGATATTTGTTAAATCTAAAGGAAAGGACTTGCACACTTTCAAAAGAATCCTAAAGTGGTACGAGGAAGCTTCGGGCCAAACTATCAATTTGGAGAATTCGTCCTTCATGGCTAGCAAGAATCTAAGTAAGGAAGCTAGGAATGAGTGCGAGAGAATCCTGAGGATAGAGAAAACCGATTCGCTAGGCCACTATTTGGGAATGTCGACTTAA

Protein sequence

MRSMTFSDNWIERIMSCISTVSYSIMINGSPHDFFKPSRGLRQGDPLSPYLFLRHAEGFSALLKREESLSNIVGFQINKYCPLLTHLFFVDDSLIFVKSKGKDLHTFKRILKWYEEASGQTINLENSSFMASKNLSKEARNECERILRIEKTDSLGHYLGMST
Homology
BLAST of Lag0009398 vs. NCBI nr
Match: XP_022157437.1 (uncharacterized protein LOC111024135 [Momordica charantia])

HSP 1 Score: 157.9 bits (398), Expect = 7.3e-35
Identity = 76/162 (46.91%), Postives = 109/162 (67.28%), Query Frame = 0

Query: 1   MRSMTFSDNWIERIMSCISTVSYSIMINGSPHDFFKPSRGLRQGDPLSPYLFLRHAEGFS 60
           M  M F++ W++ IM+C+ +V ++++ING P D F P+RGLRQGDPLSPYLF+  AEG S
Sbjct: 413 MVKMGFANRWVDLIMNCVESVRFAVLINGVPGDEFTPNRGLRQGDPLSPYLFIMCAEGLS 472

Query: 61  ALLKREESLSNIVGFQINKYCPLLTHLFFVDDSLIFVKSKGKDLHTFKRILKWYEEA-SG 120
           AL+  EE   NI   +IN+ CP ++HLF+ DD L+F K+   +  + K IL+ YE+A SG
Sbjct: 473 ALINDEEKKGNITRLKINRRCPPISHLFYADDCLLFFKASTGNCRSIKGILESYEKASSG 532

Query: 121 QTINLENSSFMASKNLSKEARNECERILRIEKTDSLGHYLGM 162
           Q INL+ S F+ SKN  ++      + L +  T+SLG YLG+
Sbjct: 533 QCINLDKSMFIVSKNTKEQMVGVIRQELMVNHTESLGQYLGL 574

BLAST of Lag0009398 vs. NCBI nr
Match: XP_019170410.1 (PREDICTED: uncharacterized protein LOC109165884 [Ipomoea nil])

HSP 1 Score: 156.4 bits (394), Expect = 2.1e-34
Identity = 75/161 (46.58%), Postives = 104/161 (64.60%), Query Frame = 0

Query: 1   MRSMTFSDNWIERIMSCISTVSYSIMINGSPHDFFKPSRGLRQGDPLSPYLFLRHAEGFS 60
           + ++ F D W++ +M C+STVSYS++INGS  +   P+RGLRQGDPLSPYLF+  AEG S
Sbjct: 355 LEALGFDDRWVKFLMMCVSTVSYSVLINGSKSEQITPTRGLRQGDPLSPYLFIICAEGLS 414

Query: 61  ALLKREESLSNIVGFQINKYCPLLTHLFFVDDSLIFVKSKGKDLHTFKRILKWYEEASGQ 120
            LLK+ E   +I G ++ +  P +THLFF DDSL+F K+  ++    KR L  YE  SGQ
Sbjct: 415 ILLKQAEVKGDIHGCRVARGAPSITHLFFADDSLLFFKANNQEAGAIKRCLTTYETMSGQ 474

Query: 121 TINLENSSFMASKNLSKEARNECERILRIEKTDSLGHYLGM 162
            +N   S+   SKN S+E R E   IL + +  + G YLG+
Sbjct: 475 AVNYHKSNICFSKNTSEEVREEIVDILGVVRAQNFGKYLGL 515

BLAST of Lag0009398 vs. NCBI nr
Match: XP_027185015.1 (uncharacterized protein LOC113783152 [Coffea eugenioides])

HSP 1 Score: 155.2 bits (391), Expect = 4.8e-34
Identity = 79/163 (48.47%), Postives = 107/163 (65.64%), Query Frame = 0

Query: 1   MRSMTFSDNWIERIMSCISTVSYSIMINGSPHDFFKPSRGLRQGDPLSPYLFLRHAEGFS 60
           M  M F + WI  IM CISTVS+S  ING   ++  P RG+RQGDPLSPYLFL  +EGFS
Sbjct: 540 MEKMRFCEKWITWIMKCISTVSFSFNINGECKEYIVPERGIRQGDPLSPYLFLICSEGFS 599

Query: 61  ALLKREESLSNIVGFQINKYCPLLTHLFFVDDSLIFVKSKGKDLHTFKRILKWYEEASGQ 120
           +LLKR     N+ G +I+++ P +THLFF DDSLIF K+  ++    KRIL+ YE+ SGQ
Sbjct: 600 SLLKRATQTRNMAGMKISRHGPSITHLFFADDSLIFCKANSQEAKELKRILEVYEQESGQ 659

Query: 121 TINLENSSFMASKNLSKEARNE-CERILRIEKTDSLGHYLGMS 163
            +NL+ SS   SKN++   + + C  +  I+   S G YLG++
Sbjct: 660 MVNLDKSSVFFSKNVNLATKEDVCTELGGIQPV-SQGKYLGLA 701

BLAST of Lag0009398 vs. NCBI nr
Match: XP_024172304.2 (uncharacterized protein LOC112178381 [Rosa chinensis])

HSP 1 Score: 154.5 bits (389), Expect = 8.1e-34
Identity = 74/163 (45.40%), Postives = 99/163 (60.74%), Query Frame = 0

Query: 1   MRSMTFSDNWIERIMSCISTVSYSIMINGSPHDFFKPSRGLRQGDPLSPYLFLRHAEGFS 60
           MRSM F   WI+ IM C++TVSYS ++NG P     P+RGLRQGD +SPYLFL  AEG S
Sbjct: 610 MRSMGFDQIWIKWIMGCVTTVSYSFLLNGEPRGHLIPTRGLRQGDSISPYLFLLCAEGLS 669

Query: 61  ALLKREESLSNIVGFQINKYCPLLTHLFFVDDSLIFVKSKGKDLHTFKRILKWYEEASGQ 120
            +L  EE    + G  I    P + HLFF DDS +F+K++ ++    K ILKWYE+ASGQ
Sbjct: 670 RMLSYEEEQHRLHGIAIAMGAPSINHLFFADDSFVFMKAEREECARVKEILKWYEDASGQ 729

Query: 121 TINLENSSFMASKNLSKEARNECERILRIEKTDSLGHYLGMST 164
            +N + S    SKN+    + E   +  +E+ D    YLG+ T
Sbjct: 730 QVNFQKSKISFSKNVDIGCQEELAEVFGVERVDKHDKYLGLPT 772

BLAST of Lag0009398 vs. NCBI nr
Match: XP_024156142.1 (uncharacterized protein LOC112164137 [Rosa chinensis])

HSP 1 Score: 154.1 bits (388), Expect = 1.1e-33
Identity = 74/163 (45.40%), Postives = 98/163 (60.12%), Query Frame = 0

Query: 1   MRSMTFSDNWIERIMSCISTVSYSIMINGSPHDFFKPSRGLRQGDPLSPYLFLRHAEGFS 60
           MRSM F   WI  IM C++TVSYS ++NG P     P+RGLRQGD +SPYLFL  AEG S
Sbjct: 402 MRSMGFDQIWINWIMGCVTTVSYSFLLNGEPRGHLIPTRGLRQGDSISPYLFLLCAEGLS 461

Query: 61  ALLKREESLSNIVGFQINKYCPLLTHLFFVDDSLIFVKSKGKDLHTFKRILKWYEEASGQ 120
            +L  EE    + G  I    P + HLFF DDS +F+K++ ++    K ILKWYE+ASGQ
Sbjct: 462 RMLSYEEEQHRLHGIAIAMGAPSINHLFFADDSFVFMKAEREECARVKEILKWYEDASGQ 521

Query: 121 TINLENSSFMASKNLSKEARNECERILRIEKTDSLGHYLGMST 164
            +N + S    SKN+    + E   +  +E+ D    YLG+ T
Sbjct: 522 QVNFQKSKISFSKNVDIGCQEELAEVFGVERVDKHDKYLGLPT 564

BLAST of Lag0009398 vs. ExPASy Swiss-Prot
Match: P92555 (Uncharacterized mitochondrial protein AtMg01250 OS=Arabidopsis thaliana OX=3702 GN=AtMg01250 PE=4 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 9.1e-12
Identity = 31/68 (45.59%), Postives = 42/68 (61.76%), Query Frame = 0

Query: 26 MINGSPHDFFKPSRGLRQGDPLSPYLFLRHAEGFSALLKREESLSNIVGFQINKYCPLLT 85
          +ING+P     PSRGLRQGDPLSPYLF+   E  S L +R +    + G +++   P + 
Sbjct: 13 IINGAPQGLVTPSRGLRQGDPLSPYLFILCTEVLSGLCRRAQEQGRLPGIRVSNNSPRIN 72

Query: 86 HLFFVDDS 94
          HL F DD+
Sbjct: 73 HLLFADDT 80

BLAST of Lag0009398 vs. ExPASy Swiss-Prot
Match: P11369 (LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE=1 SV=2)

HSP 1 Score: 48.1 bits (113), Expect = 1.1e-04
Identity = 39/154 (25.32%), Postives = 67/154 (43.51%), Query Frame = 0

Query: 10  WIERIMSCISTVSYSIMINGSPHDFFKPSRGLRQGDPLSPYLFLRHAEGFSALLKREESL 69
           ++  I +  S    +I +NG   +      G RQG PLSPYLF    E  +  +++++  
Sbjct: 633 YLNMIKAIYSKPVANIKVNGEKLEAIPLKSGTRQGCPLSPYLFNIVLEVLARAIRQQK-- 692

Query: 70  SNIVGFQINKYCPLLTHLFFVDDSLIFVKSKGKDLHTFKRILKWYEEASGQTINLENS-S 129
             I G QI K    ++ L   DD ++++            ++  + E  G  IN   S +
Sbjct: 693 -EIKGIQIGKEEVKISLL--ADDMIVYISDPKNSTRELLNLINSFGEVVGYKINSNKSMA 752

Query: 130 FMASKNLSKEARNECERILRIEKTDSLGHYLGMS 163
           F+ +KN  K+A  E           +   YLG++
Sbjct: 753 FLYTKN--KQAEKEIRETTPFSIVTNNIKYLGVT 779

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022157437.17.3e-3546.91uncharacterized protein LOC111024135 [Momordica charantia][more]
XP_019170410.12.1e-3446.58PREDICTED: uncharacterized protein LOC109165884 [Ipomoea nil][more]
XP_027185015.14.8e-3448.47uncharacterized protein LOC113783152 [Coffea eugenioides][more]
XP_024172304.28.1e-3445.40uncharacterized protein LOC112178381 [Rosa chinensis][more]
XP_024156142.11.1e-3345.40uncharacterized protein LOC112164137 [Rosa chinensis][more]
Match NameE-valueIdentityDescription
P925559.1e-1245.59Uncharacterized mitochondrial protein AtMg01250 OS=Arabidopsis thaliana OX=3702 ... [more]
P113691.1e-0425.32LINE-1 retrotransposable element ORF2 protein OS=Mus musculus OX=10090 GN=Pol PE... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000477Reverse transcriptase domainPFAMPF00078RVT_1coord: 2..149
e-value: 1.7E-15
score: 57.2
IPR000477Reverse transcriptase domainPROSITEPS50878RT_POLcoord: 1..163
score: 8.896116
NoneNo IPR availablePANTHERPTHR33116:SF45SUBFAMILY NOT NAMEDcoord: 1..161
NoneNo IPR availablePANTHERPTHR33116REVERSE TRANSCRIPTASE ZINC-BINDING DOMAIN-CONTAINING PROTEIN-RELATED-RELATEDcoord: 1..161
IPR043502DNA/RNA polymerase superfamilySUPERFAMILY56672DNA/RNA polymerasescoord: 2..134

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0009398.1Lag0009398.1mRNA