Lag0009385 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0009385
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Niemann-Pick C1 protein
Location	chr9: 38734673 .. 38736182 (-)
RNA-Seq Expression	Lag0009385
Synteny	Lag0009385

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATAGAGTAATTTTGGTTTGTTCTTTCATCTGCAGTTTTTCCTTTTTCAAAATATTTGTTTATTGGTAGAAGTCGTTATCTGACATATTCTCCCTTTGTTGCCTTTATCAGGTTGGAGTTGATAACATGTGTATATTAGTACATGCTGTGAAACGACAACCATTCGAGTTGACTTTAGAAGAGCGCATAAGCAGTGCGCTGGTTGAAGTTGGTCCTTCCATAACACTAGCTAGTTTGTCAGAGATCTTGGCTTTTGCCGTTGGAACTTTTGTTCCTATGCCAGCATGTCGTGTCTTTTCCATGTTTGCTGGTGAGAAGATCGTTTCCCCATCTTACTCCGTTGCCAAGAAATACTACTTGTCTTTGTCATCCCAGTTAACTGTCTTCGTTTTTTCTCTCTTTTTCCTCTTATTTAGCTTTGGCTGTGTTGCTGGACTTCATTCTTCAACTATCGGCATTTGTAGCTCTTTTAGTCTTTGATATTTTGAGAGCTGAGGATCACAGAGTCGACTGTTTTCCGTGCATAAAAGTTCCTCCACATTCTGATGAACCTAACCAAGGTTCTTATGAAAACTTTCAGAAACTGCGTTATTCTATTTAAGTTCTCAGTTATGTTATAATCTATGGTTGCTTGTCTAAGTTAGATCTACATTCCTTCCCACCTTTTAATTCTGATTAGACAATTGGAATAAATTCTAGGCTCTCAGGGGAGACCTGGGCTTCTTTCTCGGTACATGAAGGTATGTTTGTAGGTTGTGGCAGAATATTCATTTTTAAATTTGAGCAAATACATATTTGTTATTATTCTGCCAAAGGTTTGGAATACTGATACATTTTTGCTTGTGATATTCCATATGTATTAAATCGTTATTCTCTTGTAGGATGTTCATGCACCCCTTCTTGGACTTTGGGGAGTTAAGATTGCTGTTATTGTCATCTTTGTTGGCTTGACTTTAGGAAGCATTGTAAGTTTTTCATTTCCTATTGACTTTCCTTATGATTGGCCATACAGTGCACGTGACATTGGATTTGTTTTTGTTAAATTCATGCATGCTCATGCAAAGAGAGTTAATGCATGCTTGTGTATTAGTTTATGAATTGTAAACTGCTTGTGATTAAATAGAATAGTCAATACGTGGCAAGATTTAACTGCTATTACATATTCACTTTTACTCAAGTAGTTTGCATACTTTATTTAATCATTATAGGCATTATCTACAAAGATTGAGGTCGGTTTGGAACAAAAGATTGTCCTCCCACGAGATTCTTACCTTCAGGTTTGAGCTTCATTTTTAGGTTTTCAGAATTTTATTAGTAATTTTCTTTCGTTTCTTTCTTGCTTGTTCCTTGCCATCTCATTTTTCTTTTTTTCTTATAACAGGATTATTTTGATGATCTCGCAGAATATCTACGAATTGGACCACCATTATATTTTGTTGTGAAGGATTATAATTATAGGTATCTGTCTGATTTTCTATCCACGTGTTTCAATTATGAATCTGTGTGA

mRNA sequence

ATGGATAGAGTTGGAGTTGATAACATGTGTATATTAGTACATGCTGTGAAACGACAACCATTCGAGTTGACTTTAGAAGAGCGCATAAGCAGTGCGCTGGTTGAAGTTGGTCCTTCCATAACACTAGCTAGTTTGTCAGAGATCTTGGCTTTTGCCGTTGGAACTTTTGTTCCTATGCCAGCATGTCGTGTCTTTTCCATGTTTGCTGCTTTGGCTGTGTTGCTGGACTTCATTCTTCAACTATCGGCATTTGTAGCTCTTTTAGTCTTTGATATTTTGAGAGCTGAGGATCACAGAGTCGACTGTTTTCCGTGCATAAAAGTTCCTCCACATTCTGATGAACCTAACCAAGGCTCTCAGGGGAGACCTGGGCTTCTTTCTCGGTACATGAAGGATGTTCATGCACCCCTTCTTGGACTTTGGGGAGTTAAGATTGCTGTTATTGTCATCTTTGTTGGCTTGACTTTAGGAAGCATTGCATTATCTACAAAGATTGAGGTCGGTTTGGAACAAAAGATTGTCCTCCCACGAGATTCTTACCTTCAGGATTATTTTGATGATCTCGCAGAATATCTACGAATTGGACCACCATTATATTTTGTTGTGAAGGATTATAATTATAGGTATCTGTCTGATTTTCTATCCACGTGTTTCAATTATGAATCTGTGTGA

Coding sequence (CDS)

ATGGATAGAGTTGGAGTTGATAACATGTGTATATTAGTACATGCTGTGAAACGACAACCATTCGAGTTGACTTTAGAAGAGCGCATAAGCAGTGCGCTGGTTGAAGTTGGTCCTTCCATAACACTAGCTAGTTTGTCAGAGATCTTGGCTTTTGCCGTTGGAACTTTTGTTCCTATGCCAGCATGTCGTGTCTTTTCCATGTTTGCTGCTTTGGCTGTGTTGCTGGACTTCATTCTTCAACTATCGGCATTTGTAGCTCTTTTAGTCTTTGATATTTTGAGAGCTGAGGATCACAGAGTCGACTGTTTTCCGTGCATAAAAGTTCCTCCACATTCTGATGAACCTAACCAAGGCTCTCAGGGGAGACCTGGGCTTCTTTCTCGGTACATGAAGGATGTTCATGCACCCCTTCTTGGACTTTGGGGAGTTAAGATTGCTGTTATTGTCATCTTTGTTGGCTTGACTTTAGGAAGCATTGCATTATCTACAAAGATTGAGGTCGGTTTGGAACAAAAGATTGTCCTCCCACGAGATTCTTACCTTCAGGATTATTTTGATGATCTCGCAGAATATCTACGAATTGGACCACCATTATATTTTGTTGTGAAGGATTATAATTATAGGTATCTGTCTGATTTTCTATCCACGTGTTTCAATTATGAATCTGTGTGA

Protein sequence

MDRVGVDNMCILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGTFVPMPACRVFSMFAALAVLLDFILQLSAFVALLVFDILRAEDHRVDCFPCIKVPPHSDEPNQGSQGRPGLLSRYMKDVHAPLLGLWGVKIAVIVIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQDYFDDLAEYLRIGPPLYFVVKDYNYRYLSDFLSTCFNYESV

Homology

[Close]

BLAST of Lag0009385 vs. NCBI nr
Match: XP_038878641.1 (NPC intracellular cholesterol transporter 1-like [Benincasa hispida])

HSP 1 Score: 380.9 bits (977), Expect = 7.3e-102
Identity = 196/205 (95.61%), Postives = 202/205 (98.54%), Query Frame = 0


Query: 4   VGVDNMCILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGTFVPMPACR 63
           VGVDNMCILVHAVKRQP+ELTLEERISSALVEVGPSITLASLSEILAFAVG FVPMPACR
Sbjct: 713 VGVDNMCILVHAVKRQPYELTLEERISSALVEVGPSITLASLSEILAFAVGAFVPMPACR 772

Query: 64  VFSMFAALAVLLDFILQLSAFVALLVFDILRAEDHRVDCFPCIKVPPHSDEPNQG-SQGR 123
           VFSMFAALAVLLDFILQLSAFVAL+VFDILRAEDHRVDCFPCIKVPPHSDEPNQG +QGR
Sbjct: 773 VFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCIKVPPHSDEPNQGFNQGR 832

Query: 124 PGLLSRYMKDVHAPLLGLWGVKIAVIVIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQ 183
           PGLLSRYMKDVHAPLLGLWGVKI V+VIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQ
Sbjct: 833 PGLLSRYMKDVHAPLLGLWGVKIVVVVIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQ 892

Query: 184 DYFDDLAEYLRIGPPLYFVVKDYNY 208
           DYFD+LAEYLRIGPP+YFVVKDYNY
Sbjct: 893 DYFDELAEYLRIGPPVYFVVKDYNY 917

[Close]

BLAST of Lag0009385 vs. NCBI nr
Match: XP_022140528.1 (Niemann-Pick C1 protein [Momordica charantia])

HSP 1 Score: 376.3 bits (965), Expect = 1.8e-100
Identity = 196/205 (95.61%), Postives = 199/205 (97.07%), Query Frame = 0


Query: 4   VGVDNMCILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGTFVPMPACR 63
           VGVDNMCILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVG FVPMPACR
Sbjct: 712 VGVDNMCILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGAFVPMPACR 771

Query: 64  VFSMFAALAVLLDFILQLSAFVALLVFDILRAEDHRVDCFPCIKVPPHSDEPNQG-SQGR 123
           VFSMFAALAVLLDFILQLSAFVAL+VFDILRAEDHRVDCFPCIKV PHSDEPNQG +QG+
Sbjct: 772 VFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCIKVTPHSDEPNQGFNQGK 831

Query: 124 PGLLSRYMKDVHAPLLGLWGVKIAVIVIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQ 183
           PGLLS YMKDVHAPLLGLWGVKIAVIVIFVG TLG IALSTKIEVGLEQKIVLPRDSYLQ
Sbjct: 832 PGLLSWYMKDVHAPLLGLWGVKIAVIVIFVGFTLGGIALSTKIEVGLEQKIVLPRDSYLQ 891

Query: 184 DYFDDLAEYLRIGPPLYFVVKDYNY 208
           DYFDDLAEYLRIGPPLYFVVKDYNY
Sbjct: 892 DYFDDLAEYLRIGPPLYFVVKDYNY 916

[Close]

BLAST of Lag0009385 vs. NCBI nr
Match: KAA0047847.1 (Niemann-Pick C1 protein-like isoform X2 [Cucumis melo var. makuwa])

HSP 1 Score: 370.9 bits (951), Expect = 7.6e-99
Identity = 192/205 (93.66%), Postives = 199/205 (97.07%), Query Frame = 0


Query: 4   VGVDNMCILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGTFVPMPACR 63
           VGVDNMCILVHAVKRQP+EL+LE+RISSALVEVGPSITLASLSEILAFAVGTFVPMPACR
Sbjct: 694 VGVDNMCILVHAVKRQPYELSLEDRISSALVEVGPSITLASLSEILAFAVGTFVPMPACR 753

Query: 64  VFSMFAALAVLLDFILQLSAFVALLVFDILRAEDHRVDCFPCIKVPPHSDEPNQG-SQGR 123
           VFS+FAALAVLLDFILQLSAFVAL+V DILRAEDHRVDCFPCIKV PHSDEPNQG +QGR
Sbjct: 754 VFSLFAALAVLLDFILQLSAFVALIVLDILRAEDHRVDCFPCIKVHPHSDEPNQGFNQGR 813

Query: 124 PGLLSRYMKDVHAPLLGLWGVKIAVIVIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQ 183
            GLLSRYMKDVHAPLLG WGVKI V+VIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQ
Sbjct: 814 HGLLSRYMKDVHAPLLGFWGVKIVVVVIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQ 873

Query: 184 DYFDDLAEYLRIGPPLYFVVKDYNY 208
           DYFDDLAEYLRIGPPLYFVVKDYNY
Sbjct: 874 DYFDDLAEYLRIGPPLYFVVKDYNY 898

[Close]

BLAST of Lag0009385 vs. NCBI nr
Match: XP_023533350.1 (Niemann-Pick C1 protein-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 370.5 bits (950), Expect = 9.9e-99
Identity = 193/205 (94.15%), Postives = 199/205 (97.07%), Query Frame = 0


Query: 4   VGVDNMCILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGTFVPMPACR 63
           VGVDNMCILVHAVKRQPFELTLEERIS+ALVEVGPSITLASLSEILAFAVGTFVPMPACR
Sbjct: 713 VGVDNMCILVHAVKRQPFELTLEERISNALVEVGPSITLASLSEILAFAVGTFVPMPACR 772

Query: 64  VFSMFAALAVLLDFILQLSAFVALLVFDILRAEDHRVDCFPCIKVPPHSDEPNQG-SQGR 123
           VFSMFAALAV LDFILQLSAFVAL+VFDILRAE+HRVDCFPCIKVPP SDEPNQG +Q R
Sbjct: 773 VFSMFAALAVFLDFILQLSAFVALIVFDILRAENHRVDCFPCIKVPPRSDEPNQGFNQRR 832

Query: 124 PGLLSRYMKDVHAPLLGLWGVKIAVIVIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQ 183
            GLLSRYMKDVHAPLLGLWGVKIAV++IFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQ
Sbjct: 833 LGLLSRYMKDVHAPLLGLWGVKIAVVIIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQ 892

Query: 184 DYFDDLAEYLRIGPPLYFVVKDYNY 208
            YFDDLAEYLRIGPPLYFVVKDYNY
Sbjct: 893 GYFDDLAEYLRIGPPLYFVVKDYNY 917

[Close]

BLAST of Lag0009385 vs. NCBI nr
Match: XP_011656918.1 (NPC intracellular cholesterol transporter 1 isoform X1 [Cucumis sativus])

HSP 1 Score: 369.8 bits (948), Expect = 1.7e-98
Identity = 192/205 (93.66%), Postives = 197/205 (96.10%), Query Frame = 0


Query: 4   VGVDNMCILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGTFVPMPACR 63
           VGVDNMCILVHAVKRQP+EL+LEERIS ALVEVGPSITLASLSEILAFAVGTFVPMPACR
Sbjct: 714 VGVDNMCILVHAVKRQPYELSLEERISCALVEVGPSITLASLSEILAFAVGTFVPMPACR 773

Query: 64  VFSMFAALAVLLDFILQLSAFVALLVFDILRAEDHRVDCFPCIKVPPHSDEPNQG-SQGR 123
           VFSMFAALAVLLDFILQLSAFVAL+V DILRAEDHRVDCFPCIKV PHSDEPNQG +QGR
Sbjct: 774 VFSMFAALAVLLDFILQLSAFVALIVLDILRAEDHRVDCFPCIKVHPHSDEPNQGFNQGR 833

Query: 124 PGLLSRYMKDVHAPLLGLWGVKIAVIVIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQ 183
            GLLSRYMKDVHAP LG WGVKI V+VIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQ
Sbjct: 834 HGLLSRYMKDVHAPFLGFWGVKIVVVVIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQ 893

Query: 184 DYFDDLAEYLRIGPPLYFVVKDYNY 208
           DYFDDLAEYLRIGPPLYFVVKDYNY
Sbjct: 894 DYFDDLAEYLRIGPPLYFVVKDYNY 918

[Close]

BLAST of Lag0009385 vs. ExPASy Swiss-Prot
Match: O35604 (NPC intracellular cholesterol transporter 1 OS=Mus musculus OX=10090 GN=Npc1 PE=1 SV=2)

HSP 1 Score: 149.8 bits (377), Expect = 3.6e-35
Identity = 87/207 (42.03%), Postives = 129/207 (62.32%), Query Frame = 0


Query: 4   VGVDNMCILVHAVKRQP--FELTLEERISSALVEVGPSITLASLSEILAFAVGTFVPMPA 63
           VGVDN+ ILV   +R     E TL++++   L EV P++ L+S SE  AF  G    MPA
Sbjct: 697 VGVDNIFILVQTYQRDERLQEETLDQQLGRILGEVAPTMFLSSFSETSAFFFGALSSMPA 756

Query: 64  CRVFSMFAALAVLLDFILQLSAFVALLVFDILRAEDHRVDCFPCIKVPPHSDEPNQGSQG 123
              FS+FA +AVL+DF+LQ++ FV+LL  DI R E + +D   C++      +  QGS  
Sbjct: 757 VHTFSLFAGMAVLIDFLLQITCFVSLLGLDIKRQEKNHLDILCCVR----GADDGQGSHA 816

Query: 124 RPGLLSRYMKDVHAPLLGLWGVKIAVIVIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYL 183
               L R+ K+  APLL    ++  V+ +FVG+   S+A+  K+++GL+Q + +P DSY+
Sbjct: 817 SESYLFRFFKNYFAPLLLKDWLRPIVVAVFVGVLSFSVAVVNKVDIGLDQSLSMPNDSYV 876

Query: 184 QDYFDDLAEYLRIGPPLYFVVKD-YNY 208
            DYF  LA+YL  GPP+YFV+++ YNY
Sbjct: 877 IDYFKSLAQYLHSGPPVYFVLEEGYNY 899

[Close]

BLAST of Lag0009385 vs. ExPASy Swiss-Prot
Match: P56941 (NPC intracellular cholesterol transporter 1 OS=Sus scrofa OX=9823 GN=NPC1 PE=2 SV=1)

HSP 1 Score: 149.4 bits (376), Expect = 4.7e-35
Identity = 91/217 (41.94%), Postives = 132/217 (60.83%), Query Frame = 0


Query: 4   VGVDNMCILVHAVKRQP--FELTLEERISSALVEVGPSITLASLSEILAFAVGTFVPMPA 63
           VGVDN+ ILV   +R       TL++++   L EV PS+ L+S SE +AF +G    +PA
Sbjct: 697 VGVDNIFILVQTYQRDERLQGETLDQQLGRVLGEVAPSMFLSSFSETVAFFLGGLSVVPA 756

Query: 64  CRVFSMFAALAVLLDFILQLSAFVALLVFDILRAEDHRVDCFPCIKVPPHSDEPNQGSQG 123
              FS+FA +AVL+DF+LQ++ FV+LL  DI R E +R+D   C++      E   G Q 
Sbjct: 757 VHTFSLFAGMAVLIDFLLQITCFVSLLGLDIKRQEKNRLDVVCCVQ----GAEDGAGVQA 816

Query: 124 RPGLLSRYMKDVHAPLLGLWGVKIAVIVIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYL 183
               L R+ K+ +APLL    ++  VI +FVG+   SIA+  K+E+GL+Q + +P DSY+
Sbjct: 817 SESCLFRFFKNSYAPLLLKDWMRPIVIAVFVGVLSFSIAVLNKVEIGLDQSLSMPDDSYV 876

Query: 184 QDYFDDLAEYLRIGPPLYFVVKD-YNYRYLSDFLSTC 218
            DYF  L+ YL  GPP+YFVV++ +NY  L      C
Sbjct: 877 MDYFQSLSRYLHAGPPVYFVVEEGHNYTSLKGQNMVC 909

[Close]

BLAST of Lag0009385 vs. ExPASy Swiss-Prot
Match: O15118 (NPC intracellular cholesterol transporter 1 OS=Homo sapiens OX=9606 GN=NPC1 PE=1 SV=2)

HSP 1 Score: 147.9 bits (372), Expect = 1.4e-34
Identity = 86/203 (42.36%), Postives = 128/203 (63.05%), Query Frame = 0


Query: 4   VGVDNMCILVHAVKRQP--FELTLEERISSALVEVGPSITLASLSEILAFAVGTFVPMPA 63
           VGVDN+ ILV A +R       TL++++   L EV PS+ L+S SE +AF +G    MPA
Sbjct: 697 VGVDNIFILVQAYQRDERLQGETLDQQLGRVLGEVAPSMFLSSFSETVAFFLGALSVMPA 756

Query: 64  CRVFSMFAALAVLLDFILQLSAFVALLVFDILRAEDHRVDCFPCIKVPPHSDEPNQGSQG 123
              FS+FA LAV +DF+LQ++ FV+LL  DI R E +R+D F C++      E     Q 
Sbjct: 757 VHTFSLFAGLAVFIDFLLQITCFVSLLGLDIKRQEKNRLDIFCCVR----GAEDGTSVQA 816

Query: 124 RPGLLSRYMKDVHAPLLGLWGVKIAVIVIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYL 183
               L R+ K+ ++PLL    ++  VI IFVG+   SIA+  K+++GL+Q + +P DSY+
Sbjct: 817 SESCLFRFFKNSYSPLLLKDWMRPIVIAIFVGVLSFSIAVLNKVDIGLDQSLSMPDDSYM 876

Query: 184 QDYFDDLAEYLRIGPPLYFVVKD 205
            DYF  +++YL  GPP+YFV+++
Sbjct: 877 VDYFKSISQYLHAGPPVYFVLEE 895

[Close]

BLAST of Lag0009385 vs. ExPASy Swiss-Prot
Match: Q6T3U3 (NPC1-like intracellular cholesterol transporter 1 OS=Rattus norvegicus OX=10116 GN=Npc1l1 PE=1 SV=1)

HSP 1 Score: 134.4 bits (337), Expect = 1.6e-30
Identity = 88/221 (39.82%), Postives = 128/221 (57.92%), Query Frame = 0


Query: 4   VGVDNMCILVHAVKRQPFELTLEER---ISSALVEVGPSITLASLSEILAFAVGTFVPMP 63
           VG DN+ I V   +R P  +  E+R   I   L  V PS+ L SLSE + F +G   PMP
Sbjct: 709 VGADNIFIFVLEYQRLP-RMPGEQREAHIGRTLGSVAPSMLLCSLSEAICFFLGALTPMP 768

Query: 64  ACRVFSMFAALAVLLDFILQLSAFVALLVFDILRAEDHRVD---CFPCIKVPPHSDEPNQ 123
           A R F++ + LA++LDF+LQ++AFVALL  D  R E  R D   CF   K+PP  +    
Sbjct: 769 AVRTFALTSGLAIILDFLLQMTAFVALLSLDSKRQEASRPDVLCCFSTRKLPPPKE---- 828

Query: 124 GSQGRPGLLSRYMKDVHAPLLGLWGVKIAVIVIFVGLTLGSIALSTKIEVGLEQKIVLPR 183
               + GLL R+ + ++AP L    ++  V+++F+ L   ++ L   I VGL+Q++ LP+
Sbjct: 829 ----KEGLLLRFFRKIYAPFLLHRFIRPVVMLLFLTLFGANLYLMCNINVGLDQELALPK 888

Query: 184 DSYLQDYFDDLAEYLRIGPPLYFV-VKDYNYRYLSDFLSTC 218
           DSYL DYF  L  YL +GPP+YFV    +N+   +   +TC
Sbjct: 889 DSYLIDYFLFLNRYLEVGPPVYFVTTSGFNFSSEAGMNATC 920

[Close]

BLAST of Lag0009385 vs. ExPASy Swiss-Prot
Match: Q9UHC9 (NPC1-like intracellular cholesterol transporter 1 OS=Homo sapiens OX=9606 GN=NPC1L1 PE=1 SV=2)

HSP 1 Score: 132.9 bits (333), Expect = 4.5e-30
Identity = 89/208 (42.79%), Postives = 119/208 (57.21%), Query Frame = 0


Query: 4   VGVDNMCILV---HAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGTFVPMP 63
           VG DN+ I V     + R+P E   E  I  AL  V PS+ L SLSE + F +G   PMP
Sbjct: 709 VGADNIFIFVLEYQRLPRRPGE-PREVHIGRALGRVAPSMLLCSLSEAICFFLGALTPMP 768

Query: 64  ACRVFSMFAALAVLLDFILQLSAFVALLVFDILRAEDHRVDCFPCIKVPPHSDEPNQGSQ 123
           A R F++ + LAV+LDF+LQ+SAFVALL  D  R E  R+D   C+K P     P QG  
Sbjct: 769 AVRTFALTSGLAVILDFLLQMSAFVALLSLDSKRQEASRLDVCCCVK-PQELPPPGQGE- 828

Query: 124 GRPGLLSRYMKDVHAPLLGLWGVKIAVIVIFVGLTLGSIALSTKIEVGLEQKIVLPRDSY 183
              GLL  + +  +AP L  W  +  V+++F+ L   S+     I VGL+Q++ LP+DSY
Sbjct: 829 ---GLLLGFFQKAYAPFLLHWITRGVVLLLFLALFGVSLYSMCHISVGLDQELALPKDSY 888

Query: 184 LQDYFDDLAEYLRIGPPLYFVVK-DYNY 208
           L DYF  L  Y  +G P+YFV    YN+
Sbjct: 889 LLDYFLFLNRYFEVGAPVYFVTTLGYNF 910

[Close]

BLAST of Lag0009385 vs. ExPASy TrEMBL
Match: A0A6J1CI77 (Niemann-Pick C1 protein OS=Momordica charantia OX=3673 GN=LOC111011166 PE=3 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 8.7e-101
Identity = 196/205 (95.61%), Postives = 199/205 (97.07%), Query Frame = 0


Query: 4   VGVDNMCILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGTFVPMPACR 63
           VGVDNMCILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVG FVPMPACR
Sbjct: 712 VGVDNMCILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGAFVPMPACR 771

Query: 64  VFSMFAALAVLLDFILQLSAFVALLVFDILRAEDHRVDCFPCIKVPPHSDEPNQG-SQGR 123
           VFSMFAALAVLLDFILQLSAFVAL+VFDILRAEDHRVDCFPCIKV PHSDEPNQG +QG+
Sbjct: 772 VFSMFAALAVLLDFILQLSAFVALIVFDILRAEDHRVDCFPCIKVTPHSDEPNQGFNQGK 831

Query: 124 PGLLSRYMKDVHAPLLGLWGVKIAVIVIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQ 183
           PGLLS YMKDVHAPLLGLWGVKIAVIVIFVG TLG IALSTKIEVGLEQKIVLPRDSYLQ
Sbjct: 832 PGLLSWYMKDVHAPLLGLWGVKIAVIVIFVGFTLGGIALSTKIEVGLEQKIVLPRDSYLQ 891

Query: 184 DYFDDLAEYLRIGPPLYFVVKDYNY 208
           DYFDDLAEYLRIGPPLYFVVKDYNY
Sbjct: 892 DYFDDLAEYLRIGPPLYFVVKDYNY 916

[Close]

BLAST of Lag0009385 vs. ExPASy TrEMBL
Match: A0A5A7U0V7 (Niemann-Pick C1 protein-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold133G001120 PE=3 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 3.7e-99
Identity = 192/205 (93.66%), Postives = 199/205 (97.07%), Query Frame = 0


Query: 4   VGVDNMCILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGTFVPMPACR 63
           VGVDNMCILVHAVKRQP+EL+LE+RISSALVEVGPSITLASLSEILAFAVGTFVPMPACR
Sbjct: 694 VGVDNMCILVHAVKRQPYELSLEDRISSALVEVGPSITLASLSEILAFAVGTFVPMPACR 753

Query: 64  VFSMFAALAVLLDFILQLSAFVALLVFDILRAEDHRVDCFPCIKVPPHSDEPNQG-SQGR 123
           VFS+FAALAVLLDFILQLSAFVAL+V DILRAEDHRVDCFPCIKV PHSDEPNQG +QGR
Sbjct: 754 VFSLFAALAVLLDFILQLSAFVALIVLDILRAEDHRVDCFPCIKVHPHSDEPNQGFNQGR 813

Query: 124 PGLLSRYMKDVHAPLLGLWGVKIAVIVIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQ 183
            GLLSRYMKDVHAPLLG WGVKI V+VIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQ
Sbjct: 814 HGLLSRYMKDVHAPLLGFWGVKIVVVVIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQ 873

Query: 184 DYFDDLAEYLRIGPPLYFVVKDYNY 208
           DYFDDLAEYLRIGPPLYFVVKDYNY
Sbjct: 874 DYFDDLAEYLRIGPPLYFVVKDYNY 898

[Close]

BLAST of Lag0009385 vs. ExPASy TrEMBL
Match: A0A6J1I473 (Niemann-Pick C1 protein-like OS=Cucurbita maxima OX=3661 GN=LOC111469740 PE=3 SV=1)

HSP 1 Score: 369.0 bits (946), Expect = 1.4e-98
Identity = 192/205 (93.66%), Postives = 198/205 (96.59%), Query Frame = 0


Query: 4   VGVDNMCILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGTFVPMPACR 63
           VGVDNMCILVHAVKRQPFELTLEERIS+ALVEVGPSITLASLSEILAFAVGTFVPMPACR
Sbjct: 713 VGVDNMCILVHAVKRQPFELTLEERISNALVEVGPSITLASLSEILAFAVGTFVPMPACR 772

Query: 64  VFSMFAALAVLLDFILQLSAFVALLVFDILRAEDHRVDCFPCIKVPPHSDEPNQG-SQGR 123
           VFSMFAALAV LDFILQLSAFVAL+VFDILRAE+HRVDCFPCIKVPP SDEPNQG +Q R
Sbjct: 773 VFSMFAALAVFLDFILQLSAFVALIVFDILRAENHRVDCFPCIKVPPRSDEPNQGFNQRR 832

Query: 124 PGLLSRYMKDVHAPLLGLWGVKIAVIVIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQ 183
            GLLSRYMKDVHAPLLGLWGVKIAV++IFVGLTLGSI LSTKIEVGLEQKIVLPRDSYLQ
Sbjct: 833 LGLLSRYMKDVHAPLLGLWGVKIAVVIIFVGLTLGSITLSTKIEVGLEQKIVLPRDSYLQ 892

Query: 184 DYFDDLAEYLRIGPPLYFVVKDYNY 208
            YFDDLAEYLRIGPPLYFVVKDYNY
Sbjct: 893 GYFDDLAEYLRIGPPLYFVVKDYNY 917

[Close]

BLAST of Lag0009385 vs. ExPASy TrEMBL
Match: A0A6J1G6Q4 (Niemann-Pick C1 protein-like OS=Cucurbita moschata OX=3662 GN=LOC111451262 PE=3 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 2.4e-98
Identity = 192/205 (93.66%), Postives = 198/205 (96.59%), Query Frame = 0


Query: 4   VGVDNMCILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGTFVPMPACR 63
           VGVDNMCILVHAVKRQPFELTLEERIS+ALVEVGPSITLASLSEILAFAVGTFVPMPACR
Sbjct: 713 VGVDNMCILVHAVKRQPFELTLEERISNALVEVGPSITLASLSEILAFAVGTFVPMPACR 772

Query: 64  VFSMFAALAVLLDFILQLSAFVALLVFDILRAEDHRVDCFPCIKVPPHSDEPNQG-SQGR 123
           VFSMFAALAV LDFILQLSAFVAL+VFDI RAE+HRVDCFPCIKVPP SDEPNQG +Q R
Sbjct: 773 VFSMFAALAVFLDFILQLSAFVALIVFDIRRAENHRVDCFPCIKVPPRSDEPNQGFNQRR 832

Query: 124 PGLLSRYMKDVHAPLLGLWGVKIAVIVIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQ 183
            GLLSRYMKDVHAPLLGLWGVKIAV++IFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQ
Sbjct: 833 LGLLSRYMKDVHAPLLGLWGVKIAVVIIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQ 892

Query: 184 DYFDDLAEYLRIGPPLYFVVKDYNY 208
            YFDDLAEYLRIGPPLYFVVKDYNY
Sbjct: 893 GYFDDLAEYLRIGPPLYFVVKDYNY 917

[Close]

BLAST of Lag0009385 vs. ExPASy TrEMBL
Match: A0A1S3BJ43 (Niemann-Pick C1 protein-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490460 PE=3 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 2.4e-98
Identity = 191/205 (93.17%), Postives = 198/205 (96.59%), Query Frame = 0


Query: 4   VGVDNMCILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGTFVPMPACR 63
           VGVDNMCILVHAVKRQP+EL+LE+RISSALVEVGPSITLASLSEILAFAVGTFVPMPACR
Sbjct: 713 VGVDNMCILVHAVKRQPYELSLEDRISSALVEVGPSITLASLSEILAFAVGTFVPMPACR 772

Query: 64  VFSMFAALAVLLDFILQLSAFVALLVFDILRAEDHRVDCFPCIKVPPHSDEPNQG-SQGR 123
           VFS+FAALAVLLDFILQLSAFVAL+V DILRAEDHRVDCFPCIKV PHSDEPNQG +QGR
Sbjct: 773 VFSLFAALAVLLDFILQLSAFVALIVLDILRAEDHRVDCFPCIKVHPHSDEPNQGFNQGR 832

Query: 124 PGLLSRYMKDVHAPLLGLWGVKIAVIVIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQ 183
            GLLS YMKDVHAPLLG WGVKI V+VIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQ
Sbjct: 833 HGLLSLYMKDVHAPLLGFWGVKIVVVVIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQ 892

Query: 184 DYFDDLAEYLRIGPPLYFVVKDYNY 208
           DYFDDLAEYLRIGPPLYFVVKDYNY
Sbjct: 893 DYFDDLAEYLRIGPPLYFVVKDYNY 917

[Close]

BLAST of Lag0009385 vs. TAIR 10
Match: AT4G38350.1 (Patched family protein )

HSP 1 Score: 306.6 bits (784), Expect = 1.6e-83
Identity = 152/204 (74.51%), Postives = 178/204 (87.25%), Query Frame = 0


Query: 4   VGVDNMCILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGTFVPMPACR 63
           VGVDNMCILVHAVKRQP E++LE+RISSALVEVGPSITLASLSE+LAFAVG FVPMPACR
Sbjct: 686 VGVDNMCILVHAVKRQPREVSLEQRISSALVEVGPSITLASLSEVLAFAVGAFVPMPACR 745

Query: 64  VFSMFAALAVLLDFILQLSAFVALLVFDILRAEDHRVDCFPCIKVPPHSDEPNQGSQGRP 123
           +FSMFAALA++LDF LQ++AFVAL+VFD  R+ D+R+DCFPCIKVP  S E  +G +  P
Sbjct: 746 IFSMFAALAIMLDFFLQITAFVALIVFDCKRSADNRIDCFPCIKVPSSSRESVEGGR-EP 805

Query: 124 GLLSRYMKDVHAPLLGLWGVKIAVIVIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQD 183
           G L RYMK+VHAP+LGLWGVK+ V+ +F    L SIA+S ++E GLEQKIVLPRDSYLQD
Sbjct: 806 GFLERYMKEVHAPVLGLWGVKMVVVAVFFAFALASIAISPRLETGLEQKIVLPRDSYLQD 865

Query: 184 YFDDLAEYLRIGPPLYFVVKDYNY 208
           YFD L+EYLR+GPPLYFVVK+YNY
Sbjct: 866 YFDSLSEYLRVGPPLYFVVKNYNY 888

[Close]

BLAST of Lag0009385 vs. TAIR 10
Match: AT4G38350.2 (Patched family protein )

HSP 1 Score: 306.6 bits (784), Expect = 1.6e-83
Identity = 152/204 (74.51%), Postives = 178/204 (87.25%), Query Frame = 0


Query: 4   VGVDNMCILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGTFVPMPACR 63
           VGVDNMCILVHAVKRQP E++LE+RISSALVEVGPSITLASLSE+LAFAVG FVPMPACR
Sbjct: 710 VGVDNMCILVHAVKRQPREVSLEQRISSALVEVGPSITLASLSEVLAFAVGAFVPMPACR 769

Query: 64  VFSMFAALAVLLDFILQLSAFVALLVFDILRAEDHRVDCFPCIKVPPHSDEPNQGSQGRP 123
           +FSMFAALA++LDF LQ++AFVAL+VFD  R+ D+R+DCFPCIKVP  S E  +G +  P
Sbjct: 770 IFSMFAALAIMLDFFLQITAFVALIVFDCKRSADNRIDCFPCIKVPSSSRESVEGGR-EP 829

Query: 124 GLLSRYMKDVHAPLLGLWGVKIAVIVIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQD 183
           G L RYMK+VHAP+LGLWGVK+ V+ +F    L SIA+S ++E GLEQKIVLPRDSYLQD
Sbjct: 830 GFLERYMKEVHAPVLGLWGVKMVVVAVFFAFALASIAISPRLETGLEQKIVLPRDSYLQD 889

Query: 184 YFDDLAEYLRIGPPLYFVVKDYNY 208
           YFD L+EYLR+GPPLYFVVK+YNY
Sbjct: 890 YFDSLSEYLRVGPPLYFVVKNYNY 912

[Close]

BLAST of Lag0009385 vs. TAIR 10
Match: AT1G42470.1 (Patched family protein )

HSP 1 Score: 276.2 bits (705), Expect = 2.4e-74
Identity = 146/205 (71.22%), Postives = 169/205 (82.44%), Query Frame = 0


Query: 4   VGVDNMCILVHAVKRQPFELTLEERISSALVEVGPSITLASLSEILAFAVGTFVPMPACR 63
           VGVDNMCILVHAVKRQ  EL LE RIS+AL+EVGPSITLASL+EILAFAVG F+ MPA R
Sbjct: 673 VGVDNMCILVHAVKRQEQELPLERRISNALMEVGPSITLASLAEILAFAVGAFIKMPAVR 732

Query: 64  VFSMFAALAVLLDFILQLSAFVALLVFDILRAEDHRVDCFPCIKVPPHSDEPNQG-SQGR 123
           VFSMFAALAVLLDF+LQ++AFVAL+VFD  R ED RVDCFPCIK    S    +G  Q +
Sbjct: 733 VFSMFAALAVLLDFLLQITAFVALIVFDFRRTEDKRVDCFPCIKTSKSSISAEKGVGQRK 792

Query: 124 PGLLSRYMKDVHAPLLGLWGVKIAVIVIFVGLTLGSIALSTKIEVGLEQKIVLPRDSYLQ 183
            GLL+RYMK+VHAP+L  W VKI VI  F GL +  IALST+IE GLEQ+IVLP+DSYLQ
Sbjct: 793 AGLLTRYMKEVHAPVLSHWIVKIVVIAFFFGLAMAGIALSTRIEPGLEQQIVLPQDSYLQ 852

Query: 184 DYFDDLAEYLRIGPPLYFVVKDYNY 208
            YF++++ YLRIGPPLYFV+K+YNY
Sbjct: 853 GYFNNISTYLRIGPPLYFVLKNYNY 877

The following BLAST results are available for this feature:

BLAST of Lag0009385 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038878641.1	7.3e-102	95.61	NPC intracellular cholesterol transporter 1-like [Benincasa hispida]	[more]
XP_022140528.1	1.8e-100	95.61	Niemann-Pick C1 protein [Momordica charantia]	[more]
KAA0047847.1	7.6e-99	93.66	Niemann-Pick C1 protein-like isoform X2 [Cucumis melo var. makuwa]	[more]
XP_023533350.1	9.9e-99	94.15	Niemann-Pick C1 protein-like [Cucurbita pepo subsp. pepo]	[more]
XP_011656918.1	1.7e-98	93.66	NPC intracellular cholesterol transporter 1 isoform X1 [Cucumis sativus]	[more]

BLAST of Lag0009385 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O35604	3.6e-35	42.03	NPC intracellular cholesterol transporter 1 OS=Mus musculus OX=10090 GN=Npc1 PE=...	[more]
P56941	4.7e-35	41.94	NPC intracellular cholesterol transporter 1 OS=Sus scrofa OX=9823 GN=NPC1 PE=2 S...	[more]
O15118	1.4e-34	42.36	NPC intracellular cholesterol transporter 1 OS=Homo sapiens OX=9606 GN=NPC1 PE=1...	[more]
Q6T3U3	1.6e-30	39.82	NPC1-like intracellular cholesterol transporter 1 OS=Rattus norvegicus OX=10116 ...	[more]
Q9UHC9	4.5e-30	42.79	NPC1-like intracellular cholesterol transporter 1 OS=Homo sapiens OX=9606 GN=NPC...	[more]

BLAST of Lag0009385 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1CI77	8.7e-101	95.61	Niemann-Pick C1 protein OS=Momordica charantia OX=3673 GN=LOC111011166 PE=3 SV=1	[more]
A0A5A7U0V7	3.7e-99	93.66	Niemann-Pick C1 protein-like isoform X2 OS=Cucumis melo var. makuwa OX=1194695 G...	[more]
A0A6J1I473	1.4e-98	93.66	Niemann-Pick C1 protein-like OS=Cucurbita maxima OX=3661 GN=LOC111469740 PE=3 SV...	[more]
A0A6J1G6Q4	2.4e-98	93.66	Niemann-Pick C1 protein-like OS=Cucurbita moschata OX=3662 GN=LOC111451262 PE=3 ...	[more]
A0A1S3BJ43	2.4e-98	93.17	Niemann-Pick C1 protein-like isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490460 ...	[more]

BLAST of Lag0009385 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G38350.1	1.6e-83	74.51	Patched family protein	[more]
AT4G38350.2	1.6e-83	74.51	Patched family protein	[more]
AT1G42470.1	2.4e-74	71.22	Patched family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000731	Sterol-sensing domain	PFAM	PF12349	Sterol-sensing	coord: 4..107 e-value: 4.1E-32 score: 111.1
IPR000731	Sterol-sensing domain	PROSITE	PS50156	SSD	coord: 1..90 score: 23.753395
None	No IPR available	GENE3D	1.20.1640.10	Multidrug efflux transporter AcrB transmembrane domain	coord: 4..96 e-value: 1.8E-6 score: 29.3
None	No IPR available	PANTHER	PTHR45727:SF7	PATCHED FAMILY PROTEIN	coord: 4..209
None	No IPR available	PANTHER	PTHR45727	NPC INTRACELLULAR CHOLESTEROL TRANSPORTER 1	coord: 4..209
None	No IPR available	SUPERFAMILY	82866	Multidrug efflux transporter AcrB transmembrane domain	coord: 4..90

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0009385.1	Lag0009385.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006869	lipid transport
biological_process	GO:0048868	pollen tube development
biological_process	GO:0090153	regulation of sphingolipid biosynthetic process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005319	lipid transporter activity