Lag0009284 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0009284 vs. NCBI nr
Match: XP_008457659.1 (PREDICTED: putative amidase C869.01 isoform X1 [Cucumis melo] >KAA0045712.1 putative amidase isoform X1 [Cucumis melo var. makuwa] >TYJ99568.1 putative amidase isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 802.4 bits (2071), Expect = 2.2e-228
Identity = 425/516 (82.36%), Postives = 452/516 (87.60%), Query Frame = 0

Query: 1   MAAQTLSLYISLLLILLAISSY---GSCSFDTGLSIEEATVKD----------------- 60
           MA Q+  +YIS+LL LLAI S    GSCSFDT  SIEEAT+KD                 
Sbjct: 1   MATQSFPIYISMLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVE 60

Query: 61  ------------LQRVIEVNPDALDQASKADYERRRNALGSLSPLHGIPVLLKDNIATRD 120
                       L+ +IEVNPDAL+QAS+AD +R+R++L SLSPLHGIPVL+KDNIAT+D
Sbjct: 61  FYLEQVRRLNPILKGIIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSNFRSYEPPNGWSARGGH 180
           KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWS FRSYEPPNGWSARGG 
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQ 180

Query: 181 GKNPYTLGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240
           GKNPYT+GEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA
Sbjct: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240

Query: 241 GVVPISPRQDTVGPICRTVSDAAYVLDAIVGADSYDNSTIEASKYIPRGGYGQFLRADGL 300
           GVVPIS RQDTVGPICRTV+DAAYVLDAI GAD YDNSTIEASKYIPRGGYGQFLRA+GL
Sbjct: 241 GVVPISLRQDTVGPICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGL 300

Query: 301 QGKRIGIVRKFFYFGHDDAFYPRAFEKAFKTLKQGGAILVDNLTIDSLHVITGSSSGDWT 360
           +GKRIGIVRK + FGHDD FY  AFEK FKTLKQGGAILVDNLTI+   VITGSSSG+WT
Sbjct: 301 KGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGSSSGEWT 360

Query: 361 ALLAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLKAEATKGIGD 420
           ALLAEFKISLNAYLK+LVASPIRSLSDAIEFNKKNSKLEKLREYGQELFL+AEATKGIG 
Sbjct: 361 ALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGG 420

Query: 421 AEKAALARLAKLSKNGFQRVMIKNKLDAIAAPGRLISPVLAIGGFPGVSVPAGYNPQGVP 480
           AEKAALARLAKLSK GF+R+MIKNKLDAIAAPGRLISP LAIGGFPGVSVPAGYNPQG+P
Sbjct: 421 AEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLP 480

Query: 481 FGIGFGGLKGFEPRLIEIAYGFEHLTKSRKPPPLGR 485
           FGIGFGGLKGF+PRLIEIAYGFEHLT  RK P LGR
Sbjct: 481 FGIGFGGLKGFDPRLIEIAYGFEHLTMGRKSPSLGR 516

BLAST of Lag0009284 vs. NCBI nr
Match: XP_004147024.3 (probable amidase At4g34880 [Cucumis sativus])

HSP 1 Score: 798.5 bits (2061), Expect = 3.2e-227
Identity = 420/514 (81.71%), Postives = 452/514 (87.94%), Query Frame = 0

Query: 1   MAAQTLSLYISLLLILLAI-SSYGSCSFDTGLSIEEATVKDLQ----------------- 60
           MAAQ+  +YIS+LL LLAI SSYGSCSFDT  SIEEAT+KDLQ                 
Sbjct: 1   MAAQSFPIYISMLLGLLAILSSYGSCSFDTNFSIEEATLKDLQLAFYQNKLTSTQLVEFY 60

Query: 61  ------------RVIEVNPDALDQASKADYERRRNALGSLSPLHGIPVLLKDNIATRDKL 120
                        +IEVNPDAL+QAS+AD ER+R++  SLSPLHGIPVL+KDNIAT+D+L
Sbjct: 61  LEQVRRFNPILNGIIEVNPDALNQASQADLERKRSSPRSLSPLHGIPVLVKDNIATKDQL 120

Query: 121 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSNFRSYEPPNGWSARGGHGK 180
           NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWS+FRSYE PNGWSARGG GK
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEQPNGWSARGGQGK 180

Query: 181 NPYTLGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240
           NPYT+GEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV
Sbjct: 181 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 241 VPISPRQDTVGPICRTVSDAAYVLDAIVGADSYDNSTIEASKYIPRGGYGQFLRADGLQG 300
           VPIS RQDTVGPICRTV+DAAYVLDAI G D YDNSTIEASKY+P+GGYGQFL+ DGL+G
Sbjct: 241 VPISLRQDTVGPICRTVADAAYVLDAIAGPDRYDNSTIEASKYVPKGGYGQFLKEDGLKG 300

Query: 301 KRIGIVRKFFYFGHDDAFYPRAFEKAFKTLKQGGAILVDNLTIDSLHVITGSSSGDWTAL 360
           KRIGIVRK + FGHDD FY  AFEK FKTLKQGGAILVDNLTI+S HVITGSSSG+WTA+
Sbjct: 301 KRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINSFHVITGSSSGEWTAV 360

Query: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLKAEATKGIGDAE 420
           LAEFKIS+N YLK+LVASPIRSLSDAIEFN+KNSKLEKL+EYGQELFL+AEATKGIG AE
Sbjct: 361 LAEFKISINVYLKQLVASPIRSLSDAIEFNRKNSKLEKLKEYGQELFLEAEATKGIGGAE 420

Query: 421 KAALARLAKLSKNGFQRVMIKNKLDAIAAPGRLISPVLAIGGFPGVSVPAGYNPQGVPFG 480
           KAALARLAKLSK GF+R+MIKNKLDAIAAPGRLIS  LAIGGFPGVSVPAGYNPQG+PFG
Sbjct: 421 KAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISHFLAIGGFPGVSVPAGYNPQGLPFG 480

Query: 481 IGFGGLKGFEPRLIEIAYGFEHLTKSRKPPPLGR 485
           IGFGGLKGFEPRLIEIAYGFEHLT  RK P LGR
Sbjct: 481 IGFGGLKGFEPRLIEIAYGFEHLTMGRKSPSLGR 514

BLAST of Lag0009284 vs. NCBI nr
Match: ADN34007.1 (amidase [Cucumis melo subsp. melo])

HSP 1 Score: 793.9 bits (2049), Expect = 7.9e-226
Identity = 415/501 (82.83%), Postives = 442/501 (88.22%), Query Frame = 0

Query: 13  LLILLAISSYGSCSFDTGLSIEEATVKD-----------------------------LQR 72
           LL +L++   GSCSFDT  SIEEAT+KD                             L+ 
Sbjct: 5   LLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVEFYLEQVRRLNPILKG 64

Query: 73  VIEVNPDALDQASKADYERRRNALGSLSPLHGIPVLLKDNIATRDKLNTTAGSFALLGSI 132
           +IEVNPDAL+QAS+AD +R+R++L SLSPLHGIPVL+KDNIAT+DKLNTTAGSFALLGSI
Sbjct: 65  IIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKDKLNTTAGSFALLGSI 124

Query: 133 VPRDAGVVTKLRKAGAIIFGKASLSEWSNFRSYEPPNGWSARGGHGKNPYTLGEPCGSSS 192
           VPRDAGVVTKLRKAGAIIFGKASLSEWS FRSYEPPNGWSARGG GKNPYT+GEPCGSSS
Sbjct: 125 VPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQGKNPYTMGEPCGSSS 184

Query: 193 GSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISPRQDTVGPI 252
           GSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPIS RQDTVGPI
Sbjct: 185 GSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISLRQDTVGPI 244

Query: 253 CRTVSDAAYVLDAIVGADSYDNSTIEASKYIPRGGYGQFLRADGLQGKRIGIVRKFFYFG 312
           CRTV+DAAYVLDAI GAD YDNSTIEASKYIPRGGYGQFLRA+GL+GKRIGIVRK + FG
Sbjct: 245 CRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGLKGKRIGIVRKLYDFG 304

Query: 313 HDDAFYPRAFEKAFKTLKQGGAILVDNLTIDSLHVITGSSSGDWTALLAEFKISLNAYLK 372
           HDD FY  AFEK FKTLKQGGAILVDNLTI+   VITGSSSG+WTALLAEFKISLNAYLK
Sbjct: 305 HDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGSSSGEWTALLAEFKISLNAYLK 364

Query: 373 ELVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLKAEATKGIGDAEKAALARLAKLSKN 432
           +LVASPIRSLSDAIEFNKKNSKLEKLREYGQELFL+AEATKGIG AEKAALARLAKLSK 
Sbjct: 365 QLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGGAEKAALARLAKLSKE 424

Query: 433 GFQRVMIKNKLDAIAAPGRLISPVLAIGGFPGVSVPAGYNPQGVPFGIGFGGLKGFEPRL 485
           GF+R+MIKNKLDAIAAPGRLISP LAIGGFPGVSVPAGYNPQG+PFGIGFGGLKGF+PRL
Sbjct: 425 GFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLPFGIGFGGLKGFDPRL 484

BLAST of Lag0009284 vs. NCBI nr
Match: XP_038900912.1 (LOW QUALITY PROTEIN: probable amidase At4g34880 [Benincasa hispida])

HSP 1 Score: 790.0 bits (2039), Expect = 1.1e-224
Identity = 422/514 (82.10%), Postives = 448/514 (87.16%), Query Frame = 0

Query: 1   MAAQTLSLYISLLLILLAI-SSYGSCSFDTGLSIEEATVKDLQ----------------- 60
           MAAQ+  LYIS+LL LLAI SSYGSCSFDT LSIEEATVKDLQ                 
Sbjct: 6   MAAQSFWLYISMLLSLLAILSSYGSCSFDTHLSIEEATVKDLQLAFYQNKLISRQLVKFY 65

Query: 61  ------------RVIEVNPDALDQASKADYERRRNALGSLSPLHGIPVLLKDNIATRDKL 120
                        +IEVNPDALDQAS+AD ER+RN+  SLSPLHGIPVL+KDNIATRDKL
Sbjct: 66  LNXVRRFNPILKGIIEVNPDALDQASQADLERKRNSPSSLSPLHGIPVLVKDNIATRDKL 125

Query: 121 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSNFRSYEPPNGWSARGGHGK 180
           NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLS+WS FRSY  P+GWSARGG GK
Sbjct: 126 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSKWSYFRSYXIPSGWSARGGQGK 185

Query: 181 NPYTLGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240
           NPYT+GEPCGSS GSAIS+AANMV VSLGTETDGSILCPS LNSVVGIKPTVGLTSRAGV
Sbjct: 186 NPYTMGEPCGSSGGSAISIAANMVAVSLGTETDGSILCPSILNSVVGIKPTVGLTSRAGV 245

Query: 241 VPISPRQDTVGPICRTVSDAAYVLDAIVGADSYDNSTIEASKYIPRGGYGQFLRADGLQG 300
           +PIS RQDTVGPICRTVSD AYVL AIVGAD  DNSTIEASKYIPRGGY QFLRADGL+G
Sbjct: 246 IPISLRQDTVGPICRTVSDDAYVLGAIVGADKNDNSTIEASKYIPRGGYAQFLRADGLKG 305

Query: 301 KRIGIVRKFFYFGHDDAFYPRAFEKAFKTLKQGGAILVDNLTIDSLHVITGSSSGDWTAL 360
           KRIGIVRKFF FGHDDAFYPRA+EK FKTL+QGGA+ VDNLTIDSL VI GSSSG+ TAL
Sbjct: 306 KRIGIVRKFFDFGHDDAFYPRAYEKVFKTLRQGGAVFVDNLTIDSLQVIMGSSSGEETAL 365

Query: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLKAEATKGIGDAE 420
           LAEFKISLNAYLK+LVASPIRSLSDAIEFN+KNSKLEKLREYGQ LF++AEATKGIGDAE
Sbjct: 366 LAEFKISLNAYLKQLVASPIRSLSDAIEFNEKNSKLEKLREYGQNLFIEAEATKGIGDAE 425

Query: 421 KAALARLAKLSKNGFQRVMIKNKLDAIAAPGRLISPVLAIGGFPGVSVPAGYNPQGVPFG 480
           KAALARLAKLSK+GF+RVM+KNKLDAIAAPG  IS VLAIGGFPG+SVPAGYN QGVPFG
Sbjct: 426 KAALARLAKLSKDGFERVMLKNKLDAIAAPGWEISSVLAIGGFPGISVPAGYNSQGVPFG 485

Query: 481 IGFGGLKGFEPRLIEIAYGFEHLTKSRKPPPLGR 485
           I FGGLKGFEPRLIEIAYGFEHLTKSRK P + R
Sbjct: 486 ISFGGLKGFEPRLIEIAYGFEHLTKSRKSPSIKR 519

BLAST of Lag0009284 vs. NCBI nr
Match: XP_008457662.1 (PREDICTED: putative amidase C869.01 isoform X2 [Cucumis melo])

HSP 1 Score: 786.9 bits (2031), Expect = 9.6e-224
Identity = 421/516 (81.59%), Postives = 448/516 (86.82%), Query Frame = 0

Query: 1   MAAQTLSLYISLLLILLAISSY---GSCSFDTGLSIEEATVKD----------------- 60
           MA Q+  +YIS+LL LLAI S    GSCSFDT  SIEEAT+KD                 
Sbjct: 1   MATQSFPIYISMLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVE 60

Query: 61  ------------LQRVIEVNPDALDQASKADYERRRNALGSLSPLHGIPVLLKDNIATRD 120
                       L+ +IEVNPDAL+QAS+AD +R+R++L SLSPLHGIPVL+KDNIAT+D
Sbjct: 61  FYLEQVRRLNPILKGIIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSNFRSYEPPNGWSARGGH 180
           KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWS FRSYEPPNGWSARGG 
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQ 180

Query: 181 GKNPYTLGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240
           GKNPYT+GEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA
Sbjct: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240

Query: 241 GVVPISPRQDTVGPICRTVSDAAYVLDAIVGADSYDNSTIEASKYIPRGGYGQFLRADGL 300
           GVVPIS RQDTVG    TV+DAAYVLDAI GAD YDNSTIEASKYIPRGGYGQFLRA+GL
Sbjct: 241 GVVPISLRQDTVG----TVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGL 300

Query: 301 QGKRIGIVRKFFYFGHDDAFYPRAFEKAFKTLKQGGAILVDNLTIDSLHVITGSSSGDWT 360
           +GKRIGIVRK + FGHDD FY  AFEK FKTLKQGGAILVDNLTI+   VITGSSSG+WT
Sbjct: 301 KGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGSSSGEWT 360

Query: 361 ALLAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLKAEATKGIGD 420
           ALLAEFKISLNAYLK+LVASPIRSLSDAIEFNKKNSKLEKLREYGQELFL+AEATKGIG 
Sbjct: 361 ALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGG 420

Query: 421 AEKAALARLAKLSKNGFQRVMIKNKLDAIAAPGRLISPVLAIGGFPGVSVPAGYNPQGVP 480
           AEKAALARLAKLSK GF+R+MIKNKLDAIAAPGRLISP LAIGGFPGVSVPAGYNPQG+P
Sbjct: 421 AEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLP 480

Query: 481 FGIGFGGLKGFEPRLIEIAYGFEHLTKSRKPPPLGR 485
           FGIGFGGLKGF+PRLIEIAYGFEHLT  RK P LGR
Sbjct: 481 FGIGFGGLKGFDPRLIEIAYGFEHLTMGRKSPSLGR 512

BLAST of Lag0009284 vs. ExPASy Swiss-Prot
Match: A0A1P8B760 (Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 1.2e-144
Identity = 289/508 (56.89%), Postives = 363/508 (71.46%), Query Frame = 0

Query: 7   SLYISLLLILLAISSYGSCS---FDTGLSIEEATVKD----------------------- 66
           SL I L+L    I S GS S     +  SI+EAT++D                       
Sbjct: 11  SLLILLILSQALIMSVGSASQIRLSSTFSIQEATIEDIRVAFNEKRLTSKQLVELYLEAI 70

Query: 67  ------LQRVIEVNPDALDQASKADYERRRNALGSLSPLHGIPVLLKDNIATRDKLNTTA 126
                 L  VIE NPDAL QA  AD ER       L  LHG+PVLLKD+I+T+DKLNTTA
Sbjct: 71  SKLNPILHAVIETNPDALIQAEIADRERDLKNTTKLPILHGVPVLLKDSISTKDKLNTTA 130

Query: 127 GSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSNFRSYEPPNGWSARGGHGKNPYT 186
           GSFALLGS+V RDAGVV +LR++GA+I GKASLSEW++FRS+  P+GWSARG  GKNPY 
Sbjct: 131 GSFALLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFSIPDGWSARGLQGKNPYV 190

Query: 187 L-GEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPI 246
           L   P GSSSGSAISV AN+V VSLGTETDGSIL P++ NSVVGIKP+VGLTSRAGVVPI
Sbjct: 191 LSANPSGSSSGSAISVTANLVAVSLGTETDGSILSPASQNSVVGIKPSVGLTSRAGVVPI 250

Query: 247 SPRQDTVGPICRTVSDAAYVLDAIVGADSYDNSTIEASKYIPRGGYGQFLRADGLQGKRI 306
           S RQD++GPICRTVSDA ++LDAIVG D  D +T  AS++IP GGY QFL   GL+GKR+
Sbjct: 251 SLRQDSIGPICRTVSDAVHLLDAIVGYDPLDEATKTASEFIPEGGYKQFLTTSGLKGKRL 310

Query: 307 GIVRKFFYFGHDDAFYPRAFEKAFKTLKQGGAILVDNLTIDSLHVIT-GSSSGDWTALLA 366
           GIV K          +    +   KTL++ GAI+++NLTI ++ VI  G+ SG+  ALLA
Sbjct: 311 GIVMK----------HSSLLDHHIKTLRREGAIVINNLTIPNIEVIVGGTDSGEEIALLA 370

Query: 367 EFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLKAEATKGIGDAEKA 426
           EFK+SLNAYLKELV SP+RSL+D I +N++ ++ EK++E+GQE+FL AEAT G+G+ EK 
Sbjct: 371 EFKMSLNAYLKELVKSPVRSLADVIAYNEEFAEQEKVKEWGQEVFLTAEATSGMGEKEKT 430

Query: 427 ALARLAKLSKNGFQRVMIKNKLDAIAAPGRLISPVLAIGGFPGVSVPAGYNPQGVPFGIG 481
           AL ++ +LS+NG ++++ +NKLDAI   G  +S VLAIGG+PG++VPAGY+  GVP+GI 
Sbjct: 431 ALQKMKELSRNGIEKLIEENKLDAIVTLGSDLSSVLAIGGYPGINVPAGYDSGGVPYGIS 490

BLAST of Lag0009284 vs. ExPASy Swiss-Prot
Match: Q9URY4 (Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC869.01 PE=3 SV=1)

HSP 1 Score: 267.7 bits (683), Expect = 2.6e-70
Identity = 181/468 (38.68%), Postives = 261/468 (55.77%), Query Frame = 0

Query: 44  VIEVNPDALDQASKADYERRRNALGSLSPLHGIPVLLKDNIATRDKLNTTAGSFALLGSI 103
           ++++NPD L  AS+ D ER    +    PLHGIP ++KDN AT+DK++TTAGS+ALLGSI
Sbjct: 106 ILQLNPDVLTIASELDDERANGII--RGPLHGIPFIVKDNFATKDKMDTTAGSYALLGSI 165

Query: 104 VPRDAGVVTKLRKAGAIIFGKASLSEWSNFRSYEPPNGWSARGGHGKNPYTLG-EPCGSS 163
           VPRDA VV +LR+AGA++FG A+LSEW++ RS +   G+SARGG  + P+ L   P GSS
Sbjct: 166 VPRDAYVVKQLREAGAVLFGHATLSEWADMRSNDYSEGYSARGGQSRCPFNLTVNPGGSS 225

Query: 164 SGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISPRQDTVGP 223
           SGSAISVA+NM+  +LGTETDGSI+ P+  N VVG+KPTVGLTSR GV+P S  QDT GP
Sbjct: 226 SGSAISVASNMIAFALGTETDGSIIDPAMRNGVVGLKPTVGLTSRYGVIPESEHQDTTGP 285

Query: 224 ICRTVSDAAYVLDAIVGADSYDNSTIEASKYIPR-GGYGQFL-RADGLQGKRIGIVRKFF 283
           I RTV DA YV  ++ G D  D  T+  +   P  G Y +FL     L+G R G+  K  
Sbjct: 286 IARTVRDAVYVFQSMWGIDENDIYTLNQTGKTPEDGDYMKFLSNKTSLEGARFGLPWKRL 345

Query: 284 YFGHDDAFYPRAFEKAFKTLKQGGAILVDNLTIDSLHVITGS---------SSGDWTALL 343
           +         R  E   K +++ GAI+ +N    +L VI+           +  ++T + 
Sbjct: 346 WQNAKADEIDRLLE-VVKQIEEAGAIVYNNTNFYNLDVISNDGWNWELGSVNESEYTVVK 405

Query: 344 AEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLEKLR-------EYGQELFLKAEATK 403
            +F  ++ +YL E+  + I SL D +E+N K    E  +         GQ+ FL +    
Sbjct: 406 VDFYNNIKSYLSEVKNTEIHSLEDIVEYNNKYMGTEGGKPNIVPAFSSGQDGFLASLEWG 465

Query: 404 GI-GDAEKAALARLAKLSKN-GFQRVM---IKNKLDAIAAPGRLISPVLAI-------GG 463
           G+  +    A+  + + S++ G    +        D+    G L+    +I        G
Sbjct: 466 GVKNETYWQAVEYVRRTSQDEGIDYALNYTDPKTNDSFILNGLLVPSGTSITYQQAAKAG 525

Query: 464 FPGVSVPAGYNPQGVPFGIGFGGLKGFEPRLIEIAYGFEHLTKSRKPP 481
           +P +++P G    G PFG+G       EP+LI+     E L + +  P
Sbjct: 526 YPMITLPIGVKTNGRPFGLGIMHSAWQEPQLIKYGSAIEDLLQYKAKP 570

BLAST of Lag0009284 vs. ExPASy Swiss-Prot
Match: D4B3C8 (Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_02965 PE=1 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 3.5e-67
Identity = 175/459 (38.13%), Postives = 249/459 (54.25%), Query Frame = 0

Query: 41  LQRVIEVNPDALDQASKADYERRRNALGSLSPLHGIPVLLKDNIATRDKLNTTAGSFALL 100
           ++ V E+NPDAL  A + D ER+   L    PLHG+P+++K+NI T DK+++TAGS+A+ 
Sbjct: 123 VRAVTEINPDALTIAKQMDNERKMGKL--RGPLHGLPIVIKNNIFTDDKMSSTAGSYAIF 182

Query: 101 GSIVPRDAGVVTKLRKAGAIIFGKASLSEWSNFRSYEPPNGWSARGGHGKNPYTLG-EPC 160
           G+    DA V TKLR+AG +I GK+  S+W+NFRS    NGWSA GG     Y    +P 
Sbjct: 183 GARTSADATVATKLREAGLVIMGKSGASQWANFRSLNSTNGWSAYGGQVTAAYIKNQDPS 242

Query: 161 GSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISPRQDT 220
           GSSSGS ++    +   +LGTET GSI+ P+  +++VG+KPTVGLTSR  VVPIS RQDT
Sbjct: 243 GSSSGSGVASDLGLAFATLGTETSGSIVSPADKSNIVGLKPTVGLTSRRFVVPISERQDT 302

Query: 221 VGPICRTVSDAAYVLDAIVGADSYDNSTIEASKYIPRGGYGQFLRADGLQGKRIGIVRKF 280
           VGP+ R+V DAAY+L  I G DS DN T  A  +     Y +    + L+GKRIG+ R  
Sbjct: 303 VGPMARSVKDAAYLLQVIAGKDSNDNYT-SAIPFDTIPDYVKACDINALKGKRIGVPRNV 362

Query: 281 FYFGHDDAFYPRAFEKAFKTLKQGGAILVDNLTIDSLHVITGSSSGDWTALLAEFKISLN 340
                        F +A   +K+ GAI+V+N    S      S   D   L A+   +L 
Sbjct: 363 IKIFGSPQTVVDQFNQALAVMKKAGAIIVENTDFTSFAEFAQSPIPD-DILYADSLTNLP 422

Query: 341 AYLKELVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLKAEAT--KGIGDAEKAALARL 400
           A+ K+L  +P  +++D +E  ++ ++  +L EY      + +    KGI + +       
Sbjct: 423 AFFKQLKVNP-HNITD-LESLRRFTQHHRLEEYPSRDTARWDIALQKGIKNTDPKFWPMY 482

Query: 401 AKLSK----NGFQRVMIKNKLDAIAAPGRLISPVLAIGGFPGVSVPAGYNPQ-------- 460
            K  K     G    + ++KLDA   P  L   + A+ G P ++VP G  P         
Sbjct: 483 QKNVKFGNEGGILGALRRHKLDAAVLPTDLSPYIPALIGSPIITVPMGVYPNGTKVNHDR 542

Query: 461 -------GVPFGIGFGGLKGFEPRLIEIAYGFEHLTKSR 478
                  G+P GIGF G    E +LI +AY FE  T +R
Sbjct: 543 ELVTSGPGIPIGIGFMGDLWSEEKLIGLAYAFEQKTHAR 575

BLAST of Lag0009284 vs. ExPASy Swiss-Prot
Match: Q8RC40 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Caldanaerobacter subterraneus subsp. tengcongensis (strain DSM 15242 / JCM 11007 / NBRC 100824 / MB4) OX=273068 GN=gatA PE=3 SV=1)

HSP 1 Score: 166.4 bits (420), Expect = 8.1e-40
Identity = 153/488 (31.35%), Postives = 235/488 (48.16%), Query Frame = 0

Query: 31  LSIEEATVKDLQRVIEVNPD-----------ALDQASKADYERRRNALGSLSPLHGIPVL 90
           +S +E T   L+R+ EV P            AL++A  AD E  +N  G    L GIPV+
Sbjct: 20  VSAQEVTKSYLERIKEVEPKIDALVTITEEFALERAKVAD-EMIKN--GEAKDLTGIPVI 79

Query: 91  LKDNIATRDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSNFRSYEPP 150
           +KDNI+T + + TT  S  L   I P +A VV +L   G +I GK++L E++   S E  
Sbjct: 80  IKDNIST-EGIRTTCSSKMLENYIPPYNATVVERLLNEGVVILGKSNLDEFAMGSSTE-- 139

Query: 151 NGWSARGGHGKNPYTLGE-PCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGI 210
              ++     KNP+ L   P GSS GSA +VAA+    +LG++T GSI  P++L  VVG+
Sbjct: 140 ---NSAFKTTKNPWDLSRVPGGSSGGSAAAVAADEAAFALGSDTGGSIRQPASLCGVVGM 199

Query: 211 KPTVGLTSRAGVVPISPRQDTVGPICRTVSDAAYVLDAIVGADSYDNSTIEASKYIPRGG 270
           KPT GL SR G+V  +   D +GP  + V+D A VL+AI G D  D+++++    I +  
Sbjct: 200 KPTYGLVSRYGLVAFASSLDQIGPFTKDVTDCAIVLNAIAGHDPMDSTSVK----IEKPD 259

Query: 271 YGQFLRADGLQGKRIGIVRKFFYFGHDDAFYPRAFEKAFKTLKQGGAILVDNLTIDSLHV 330
           Y  +L+ D ++G RIG+ ++FF  G ++       EKA K  +  GA ++D     S+  
Sbjct: 260 YTSYLKED-IKGLRIGVAKEFFGAGIEEG-VKETVEKAIKVFEDLGAEIID----ISVPY 319

Query: 331 ITGSSSGDWTALLAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLEKLREYGQELFL 390
           +  +    +    AE   +L  Y         ++  D ++    +      +E  + + L
Sbjct: 320 VEYALPAYYIIASAEASSNLARYDGIRYGHIAKNYEDLVDMYMTSRSEGFGKEVKRRIML 379

Query: 391 KAEA-TKGIGDAEKAALARLAKLSKNGFQRVMIKNKLDAIAAP---------GRLISPVL 450
              A + G  DA      ++  L KN F+R     K D I  P         G   +  L
Sbjct: 380 GTYALSSGYYDAYYKKALKVRTLIKNDFERAF--EKCDVIVGPTSPTVAFKIGERTNDPL 439

Query: 451 A------------IGGFPGVSVPAGYNPQGVPFGIGFGGLKGFEPRLIEIAYGFEHLTK- 484
           A            I G P +S+P G + +G+P G+   G    E R++ +AY FE   K 
Sbjct: 440 AMYLADIYTVSVNIAGLPAISIPCGLS-EGLPVGLQIIGKHFDEGRILNVAYAFEKAYKF 485

BLAST of Lag0009284 vs. ExPASy Swiss-Prot
Match: B0K3S3 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter sp. (strain X514) OX=399726 GN=gatA PE=3 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 6.8e-39
Identity = 150/488 (30.74%), Postives = 235/488 (48.16%), Query Frame = 0

Query: 31  LSIEEATVKDLQRVIEVNPD-----------ALDQASKADYERRRNALGSLSPLHGIPVL 90
           +S  E T   L+R+ EV P            AL +A +AD + ++   G  + L GIPV+
Sbjct: 20  VSALEVTKSYLERIKEVEPKIDALVTITEDFALQKAKEADEKIKK---GEDTALTGIPVI 79

Query: 91  LKDNIATRDKLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSNFRSYEPP 150
           +KDNI+T + + TT  S  L   I P +A VV KL + G II GK++L E++   S E  
Sbjct: 80  IKDNIST-EGIKTTCSSKMLENYIPPYNATVVEKLLEEGVIILGKSNLDEFAMGSSTE-- 139

Query: 151 NGWSARGGHGKNPYTLGE-PCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGI 210
              ++     KNP+ L   P GSS GSA ++AA+    +LG++T GSI  P++L  VVG+
Sbjct: 140 ---NSAFKTTKNPWDLSRVPGGSSGGSAAAIAADEAAFALGSDTGGSIRQPASLCGVVGM 199

Query: 211 KPTVGLTSRAGVVPISPRQDTVGPICRTVSDAAYVLDAIVGADSYDNSTIEASKYIPRGG 270
           KPT GL SR G+V  +   D +GP  + V+D A VL+ I+G D  D+++++    I +  
Sbjct: 200 KPTYGLVSRYGLVAFASSLDQIGPFTKDVTDCAIVLNTIIGHDPKDSTSLK----IDKPD 259

Query: 271 YGQFLRADGLQGKRIGIVRKFFYFGHDDAFYPRAFEKAFKTLKQGGAILVDNLTIDSLHV 330
           Y  +L+ D ++G RIG+ ++FF  G ++       +++ K L+  GA ++D     S+  
Sbjct: 260 YTSYLKED-IKGLRIGVAKEFFGEGIEEG-VKETVQESIKVLQDLGAEIID----ISIPY 319

Query: 331 ITGSSSGDWTALLAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLEKLREYGQELFL 390
           +  +    +    AE   +L  Y             D I+           +E  + + L
Sbjct: 320 VEYALPAYYIIASAEASSNLARYDGIRYGHIAEKYEDLIDMYMVTRSEGFGKEVKRRIML 379

Query: 391 KAEA-TKGIGDAEKAALARLAKLSKNGFQRVMIKNKLDAIAAP---------GRLISPVL 450
              A + G  DA      ++  L KN F++     K D I  P         G   +  L
Sbjct: 380 GTYALSSGYYDAYYKKALKVRTLIKNDFEKAF--EKCDVIIGPTSPTVAFKIGERANDPL 439

Query: 451 A------------IGGFPGVSVPAGYNPQGVPFGIGFGGLKGFEPRLIEIAYGFEHLTK- 484
           A            I G PG+S+P G +  G+P G+   G    E +++ +AY FE   K 
Sbjct: 440 AMYLADIYTVSVNIAGLPGISIPCGLS-DGLPVGLQIIGRHFDEGKILNVAYAFEQANKF 485

BLAST of Lag0009284 vs. ExPASy TrEMBL
Match: A0A5D3BN38 (Putative amidase isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G001380 PE=4 SV=1)

HSP 1 Score: 802.4 bits (2071), Expect = 1.1e-228
Identity = 425/516 (82.36%), Postives = 452/516 (87.60%), Query Frame = 0

Query: 1   MAAQTLSLYISLLLILLAISSY---GSCSFDTGLSIEEATVKD----------------- 60
           MA Q+  +YIS+LL LLAI S    GSCSFDT  SIEEAT+KD                 
Sbjct: 1   MATQSFPIYISMLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVE 60

Query: 61  ------------LQRVIEVNPDALDQASKADYERRRNALGSLSPLHGIPVLLKDNIATRD 120
                       L+ +IEVNPDAL+QAS+AD +R+R++L SLSPLHGIPVL+KDNIAT+D
Sbjct: 61  FYLEQVRRLNPILKGIIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSNFRSYEPPNGWSARGGH 180
           KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWS FRSYEPPNGWSARGG 
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQ 180

Query: 181 GKNPYTLGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240
           GKNPYT+GEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA
Sbjct: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240

Query: 241 GVVPISPRQDTVGPICRTVSDAAYVLDAIVGADSYDNSTIEASKYIPRGGYGQFLRADGL 300
           GVVPIS RQDTVGPICRTV+DAAYVLDAI GAD YDNSTIEASKYIPRGGYGQFLRA+GL
Sbjct: 241 GVVPISLRQDTVGPICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGL 300

Query: 301 QGKRIGIVRKFFYFGHDDAFYPRAFEKAFKTLKQGGAILVDNLTIDSLHVITGSSSGDWT 360
           +GKRIGIVRK + FGHDD FY  AFEK FKTLKQGGAILVDNLTI+   VITGSSSG+WT
Sbjct: 301 KGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGSSSGEWT 360

Query: 361 ALLAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLKAEATKGIGD 420
           ALLAEFKISLNAYLK+LVASPIRSLSDAIEFNKKNSKLEKLREYGQELFL+AEATKGIG 
Sbjct: 361 ALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGG 420

Query: 421 AEKAALARLAKLSKNGFQRVMIKNKLDAIAAPGRLISPVLAIGGFPGVSVPAGYNPQGVP 480
           AEKAALARLAKLSK GF+R+MIKNKLDAIAAPGRLISP LAIGGFPGVSVPAGYNPQG+P
Sbjct: 421 AEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLP 480

Query: 481 FGIGFGGLKGFEPRLIEIAYGFEHLTKSRKPPPLGR 485
           FGIGFGGLKGF+PRLIEIAYGFEHLT  RK P LGR
Sbjct: 481 FGIGFGGLKGFDPRLIEIAYGFEHLTMGRKSPSLGR 516

BLAST of Lag0009284 vs. ExPASy TrEMBL
Match: A0A1S3C7A8 (putative amidase C869.01 isoform X1 OS=Cucumis melo OX=3656 GN=LOC107990287 PE=4 SV=1)

HSP 1 Score: 802.4 bits (2071), Expect = 1.1e-228
Identity = 425/516 (82.36%), Postives = 452/516 (87.60%), Query Frame = 0

Query: 1   MAAQTLSLYISLLLILLAISSY---GSCSFDTGLSIEEATVKD----------------- 60
           MA Q+  +YIS+LL LLAI S    GSCSFDT  SIEEAT+KD                 
Sbjct: 1   MATQSFPIYISMLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVE 60

Query: 61  ------------LQRVIEVNPDALDQASKADYERRRNALGSLSPLHGIPVLLKDNIATRD 120
                       L+ +IEVNPDAL+QAS+AD +R+R++L SLSPLHGIPVL+KDNIAT+D
Sbjct: 61  FYLEQVRRLNPILKGIIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSNFRSYEPPNGWSARGGH 180
           KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWS FRSYEPPNGWSARGG 
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQ 180

Query: 181 GKNPYTLGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240
           GKNPYT+GEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA
Sbjct: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240

Query: 241 GVVPISPRQDTVGPICRTVSDAAYVLDAIVGADSYDNSTIEASKYIPRGGYGQFLRADGL 300
           GVVPIS RQDTVGPICRTV+DAAYVLDAI GAD YDNSTIEASKYIPRGGYGQFLRA+GL
Sbjct: 241 GVVPISLRQDTVGPICRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGL 300

Query: 301 QGKRIGIVRKFFYFGHDDAFYPRAFEKAFKTLKQGGAILVDNLTIDSLHVITGSSSGDWT 360
           +GKRIGIVRK + FGHDD FY  AFEK FKTLKQGGAILVDNLTI+   VITGSSSG+WT
Sbjct: 301 KGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGSSSGEWT 360

Query: 361 ALLAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLKAEATKGIGD 420
           ALLAEFKISLNAYLK+LVASPIRSLSDAIEFNKKNSKLEKLREYGQELFL+AEATKGIG 
Sbjct: 361 ALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGG 420

Query: 421 AEKAALARLAKLSKNGFQRVMIKNKLDAIAAPGRLISPVLAIGGFPGVSVPAGYNPQGVP 480
           AEKAALARLAKLSK GF+R+MIKNKLDAIAAPGRLISP LAIGGFPGVSVPAGYNPQG+P
Sbjct: 421 AEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLP 480

Query: 481 FGIGFGGLKGFEPRLIEIAYGFEHLTKSRKPPPLGR 485
           FGIGFGGLKGF+PRLIEIAYGFEHLT  RK P LGR
Sbjct: 481 FGIGFGGLKGFDPRLIEIAYGFEHLTMGRKSPSLGR 516

BLAST of Lag0009284 vs. ExPASy TrEMBL
Match: A0A0A0LLK0 (Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G264040 PE=4 SV=1)

HSP 1 Score: 797.0 bits (2057), Expect = 4.5e-227
Identity = 419/514 (81.52%), Postives = 451/514 (87.74%), Query Frame = 0

Query: 1   MAAQTLSLYISLLLILLAI-SSYGSCSFDTGLSIEEATVKDLQ----------------- 60
           MAAQ+  +YIS+LL LLAI SSYGSCSFDT  SIEEAT+KDLQ                 
Sbjct: 1   MAAQSFPIYISMLLGLLAILSSYGSCSFDTNFSIEEATLKDLQLAFYQNKLTSTQLVEFY 60

Query: 61  ------------RVIEVNPDALDQASKADYERRRNALGSLSPLHGIPVLLKDNIATRDKL 120
                        +IEVNPDAL+QAS+AD ER+R++  SLSPLHGIPV +KDNIAT+D+L
Sbjct: 61  LEQVRRFNPILNGIIEVNPDALNQASQADLERKRSSPRSLSPLHGIPVFVKDNIATKDQL 120

Query: 121 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSNFRSYEPPNGWSARGGHGK 180
           NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWS+FRSYE PNGWSARGG GK
Sbjct: 121 NTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSDFRSYEQPNGWSARGGQGK 180

Query: 181 NPYTLGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240
           NPYT+GEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV
Sbjct: 181 NPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 240

Query: 241 VPISPRQDTVGPICRTVSDAAYVLDAIVGADSYDNSTIEASKYIPRGGYGQFLRADGLQG 300
           VPIS RQDTVGPICRTV+DAAYVLDAI G D YDNSTIEASKY+P+GGYGQFL+ DGL+G
Sbjct: 241 VPISLRQDTVGPICRTVADAAYVLDAIAGPDRYDNSTIEASKYVPKGGYGQFLKEDGLKG 300

Query: 301 KRIGIVRKFFYFGHDDAFYPRAFEKAFKTLKQGGAILVDNLTIDSLHVITGSSSGDWTAL 360
           KRIGIVRK + FGHDD FY  AFEK FKTLKQGGAILVDNLTI+S HVITGSSSG+WTA+
Sbjct: 301 KRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINSFHVITGSSSGEWTAV 360

Query: 361 LAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLKAEATKGIGDAE 420
           LAEFKIS+N YLK+LVASPIRSLSDAIEFN+KNSKLEKL+EYGQELFL+AEATKGIG AE
Sbjct: 361 LAEFKISINVYLKQLVASPIRSLSDAIEFNRKNSKLEKLKEYGQELFLEAEATKGIGGAE 420

Query: 421 KAALARLAKLSKNGFQRVMIKNKLDAIAAPGRLISPVLAIGGFPGVSVPAGYNPQGVPFG 480
           KAALARLAKLSK GF+R+MIKNKLDAIAAPGRLIS  LAIGGFPGVSVPAGYNPQG+PFG
Sbjct: 421 KAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISHFLAIGGFPGVSVPAGYNPQGLPFG 480

Query: 481 IGFGGLKGFEPRLIEIAYGFEHLTKSRKPPPLGR 485
           IGFGGLKGFEPRLIEIAYGFEHLT  RK P LGR
Sbjct: 481 IGFGGLKGFEPRLIEIAYGFEHLTMGRKSPSLGR 514

BLAST of Lag0009284 vs. ExPASy TrEMBL
Match: E5GC08 (Amidase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 793.9 bits (2049), Expect = 3.8e-226
Identity = 415/501 (82.83%), Postives = 442/501 (88.22%), Query Frame = 0

Query: 13  LLILLAISSYGSCSFDTGLSIEEATVKD-----------------------------LQR 72
           LL +L++   GSCSFDT  SIEEAT+KD                             L+ 
Sbjct: 5   LLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVEFYLEQVRRLNPILKG 64

Query: 73  VIEVNPDALDQASKADYERRRNALGSLSPLHGIPVLLKDNIATRDKLNTTAGSFALLGSI 132
           +IEVNPDAL+QAS+AD +R+R++L SLSPLHGIPVL+KDNIAT+DKLNTTAGSFALLGSI
Sbjct: 65  IIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKDKLNTTAGSFALLGSI 124

Query: 133 VPRDAGVVTKLRKAGAIIFGKASLSEWSNFRSYEPPNGWSARGGHGKNPYTLGEPCGSSS 192
           VPRDAGVVTKLRKAGAIIFGKASLSEWS FRSYEPPNGWSARGG GKNPYT+GEPCGSSS
Sbjct: 125 VPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQGKNPYTMGEPCGSSS 184

Query: 193 GSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISPRQDTVGPI 252
           GSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPIS RQDTVGPI
Sbjct: 185 GSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISLRQDTVGPI 244

Query: 253 CRTVSDAAYVLDAIVGADSYDNSTIEASKYIPRGGYGQFLRADGLQGKRIGIVRKFFYFG 312
           CRTV+DAAYVLDAI GAD YDNSTIEASKYIPRGGYGQFLRA+GL+GKRIGIVRK + FG
Sbjct: 245 CRTVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGLKGKRIGIVRKLYDFG 304

Query: 313 HDDAFYPRAFEKAFKTLKQGGAILVDNLTIDSLHVITGSSSGDWTALLAEFKISLNAYLK 372
           HDD FY  AFEK FKTLKQGGAILVDNLTI+   VITGSSSG+WTALLAEFKISLNAYLK
Sbjct: 305 HDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGSSSGEWTALLAEFKISLNAYLK 364

Query: 373 ELVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLKAEATKGIGDAEKAALARLAKLSKN 432
           +LVASPIRSLSDAIEFNKKNSKLEKLREYGQELFL+AEATKGIG AEKAALARLAKLSK 
Sbjct: 365 QLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGGAEKAALARLAKLSKE 424

Query: 433 GFQRVMIKNKLDAIAAPGRLISPVLAIGGFPGVSVPAGYNPQGVPFGIGFGGLKGFEPRL 485
           GF+R+MIKNKLDAIAAPGRLISP LAIGGFPGVSVPAGYNPQG+PFGIGFGGLKGF+PRL
Sbjct: 425 GFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLPFGIGFGGLKGFDPRL 484

BLAST of Lag0009284 vs. ExPASy TrEMBL
Match: A0A1S3C652 (putative amidase C869.01 isoform X2 OS=Cucumis melo OX=3656 GN=LOC107990287 PE=4 SV=1)

HSP 1 Score: 786.9 bits (2031), Expect = 4.6e-224
Identity = 421/516 (81.59%), Postives = 448/516 (86.82%), Query Frame = 0

Query: 1   MAAQTLSLYISLLLILLAISSY---GSCSFDTGLSIEEATVKD----------------- 60
           MA Q+  +YIS+LL LLAI S    GSCSFDT  SIEEAT+KD                 
Sbjct: 1   MATQSFPIYISMLLGLLAILSLYGSGSCSFDTNFSIEEATLKDFQLAFYQNKLTSRQLVE 60

Query: 61  ------------LQRVIEVNPDALDQASKADYERRRNALGSLSPLHGIPVLLKDNIATRD 120
                       L+ +IEVNPDAL+QAS+AD +R+R++L SLSPLHGIPVL+KDNIAT+D
Sbjct: 61  FYLEQVRRLNPILKGIIEVNPDALNQASQADLKRKRSSLRSLSPLHGIPVLVKDNIATKD 120

Query: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSNFRSYEPPNGWSARGGH 180
           KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWS FRSYEPPNGWSARGG 
Sbjct: 121 KLNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSGFRSYEPPNGWSARGGQ 180

Query: 181 GKNPYTLGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240
           GKNPYT+GEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA
Sbjct: 181 GKNPYTMGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 240

Query: 241 GVVPISPRQDTVGPICRTVSDAAYVLDAIVGADSYDNSTIEASKYIPRGGYGQFLRADGL 300
           GVVPIS RQDTVG    TV+DAAYVLDAI GAD YDNSTIEASKYIPRGGYGQFLRA+GL
Sbjct: 241 GVVPISLRQDTVG----TVADAAYVLDAIAGADRYDNSTIEASKYIPRGGYGQFLRAEGL 300

Query: 301 QGKRIGIVRKFFYFGHDDAFYPRAFEKAFKTLKQGGAILVDNLTIDSLHVITGSSSGDWT 360
           +GKRIGIVRK + FGHDD FY  AFEK FKTLKQGGAILVDNLTI+   VITGSSSG+WT
Sbjct: 301 KGKRIGIVRKLYDFGHDDVFYIGAFEKVFKTLKQGGAILVDNLTINRFDVITGSSSGEWT 360

Query: 361 ALLAEFKISLNAYLKELVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLKAEATKGIGD 420
           ALLAEFKISLNAYLK+LVASPIRSLSDAIEFNKKNSKLEKLREYGQELFL+AEATKGIG 
Sbjct: 361 ALLAEFKISLNAYLKQLVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLEAEATKGIGG 420

Query: 421 AEKAALARLAKLSKNGFQRVMIKNKLDAIAAPGRLISPVLAIGGFPGVSVPAGYNPQGVP 480
           AEKAALARLAKLSK GF+R+MIKNKLDAIAAPGRLISP LAIGGFPGVSVPAGYNPQG+P
Sbjct: 421 AEKAALARLAKLSKEGFERLMIKNKLDAIAAPGRLISPFLAIGGFPGVSVPAGYNPQGLP 480

Query: 481 FGIGFGGLKGFEPRLIEIAYGFEHLTKSRKPPPLGR 485
           FGIGFGGLKGF+PRLIEIAYGFEHLT  RK P LGR
Sbjct: 481 FGIGFGGLKGFDPRLIEIAYGFEHLTMGRKSPSLGR 512

BLAST of Lag0009284 vs. TAIR 10
Match: AT4G34880.1 (Amidase family protein )

HSP 1 Score: 436.0 bits (1120), Expect = 3.9e-122
Identity = 256/507 (50.49%), Postives = 327/507 (64.50%), Query Frame = 0

Query: 7   SLYISLLLILLAISSYGSCS---FDTGLSIEEATVKD----------------------- 66
           SL I L+L    I S GS S     +  SI+EAT++D                       
Sbjct: 11  SLLILLILSQALIMSVGSASQIRLSSTFSIQEATIEDIRVAFNEKRLTSKQLVELYLEAI 70

Query: 67  ------LQRVIEVNPDALDQASKADYERRRNALGSLSPLHGIPVLLKDNIATRDKLNTTA 126
                 L  VIE NPDAL QA  AD ER       L  LHG+PVLLKD+I+T+DKLNTTA
Sbjct: 71  SKLNPILHAVIETNPDALIQAEIADRERDLKNTTKLPILHGVPVLLKDSISTKDKLNTTA 130

Query: 127 GSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSNFRSYEPPNGWSARGGHGKNPYT 186
           GSFALLGS+V RDAGVV +LR++GA+I GKASLSEW++FRS+  P+GWSA          
Sbjct: 131 GSFALLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFSIPDGWSA---------- 190

Query: 187 LGEPCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPIS 246
                                             PS  NSVVGIKP+VGLTSRAGVVPIS
Sbjct: 191 ----------------------------------PSQ-NSVVGIKPSVGLTSRAGVVPIS 250

Query: 247 PRQDTVGPICRTVSDAAYVLDAIVGADSYDNSTIEASKYIPRGGYGQFLRADGLQGKRIG 306
            RQD++GPICRTVSDA ++LDAIVG D  D +T  AS++IP GGY QFL   GL+GKR+G
Sbjct: 251 LRQDSIGPICRTVSDAVHLLDAIVGYDPLDEATKTASEFIPEGGYKQFLTTSGLKGKRLG 310

Query: 307 IVRKFFYFGHDDAFYPRAFEKAFKTLKQGGAILVDNLTIDSLHVIT-GSSSGDWTALLAE 366
           IV K          +    +   KTL++ GAI+++NLTI ++ VI  G+ SG+  ALLAE
Sbjct: 311 IVMK----------HSSLLDHHIKTLRREGAIVINNLTIPNIEVIVGGTDSGEEIALLAE 370

Query: 367 FKISLNAYLKELVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLKAEATKGIGDAEKAA 426
           FK+SLNAYLKELV SP+RSL+D I +N++ ++ EK++E+GQE+FL AEAT G+G+ EK A
Sbjct: 371 FKMSLNAYLKELVKSPVRSLADVIAYNEEFAEQEKVKEWGQEVFLTAEATSGMGEKEKTA 430

Query: 427 LARLAKLSKNGFQRVMIKNKLDAIAAPGRLISPVLAIGGFPGVSVPAGYNPQGVPFGIGF 481
           L ++ +LS+NG ++++ +NKLDAI   G  +S VLAIGG+PG++VPAGY+  GVP+GI F
Sbjct: 431 LQKMKELSRNGIEKLIEENKLDAIVTLGSDLSSVLAIGGYPGINVPAGYDSGGVPYGISF 462

BLAST of Lag0009284 vs. TAIR 10
Match: AT3G25660.1 (Amidase family protein )

HSP 1 Score: 115.2 bits (287), Expect = 1.5e-25
Identity = 140/497 (28.17%), Postives = 221/497 (44.47%), Query Frame = 0

Query: 31  LSIEEATVKDLQRVIEVNPDALDQASKADYERRRNALG-SLSPLHGIPVLLKDNIATRDK 90
           LS    T   L+  + V+ + L  A + D   +R A G  L PL G+ + +KDNI T+  
Sbjct: 68  LSRIRLTEPQLKCFLHVSENVLKDAQEID---QRIAKGEELGPLAGVLIGVKDNICTQG- 127

Query: 91  LNTTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSNFRSYEPPNGWSARGGHG 150
           + +TA S  L     P DA  V K+++ G I+ GK ++ E+    + E     ++     
Sbjct: 128 MPSTAASRILEHYRPPFDATAVKKIKELGGIVVGKTNMDEFGMGSTTE-----ASAFQVT 187

Query: 151 KNPYTLGE-PCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRA 210
            NP+ L   P GSS GSA +VAA    VSLG++T GS+  P++   VVG+KPT G  SR 
Sbjct: 188 ANPWDLSRVPGGSSGGSAAAVAARQCMVSLGSDTGGSVRQPASFCGVVGLKPTYGRVSRF 247

Query: 211 GVVPISPRQDTVGPICRTVSDAAYVLDAIVGADSYDNSTIEASKYIPRGGYGQFLRAD-- 270
           G++  +   D +G    TV+DA  +L AI G D +D++   +SK        QFL  D  
Sbjct: 248 GLMAYASSLDVIGCFGSTVADAGMLLHAISGYDRFDST---SSKQDVPEFQSQFLSVDHF 307

Query: 271 ---GLQGKRIGIVRKFFYFGHDDAFYPRAFEKAFKTLKQGGAILVDNLTIDSLHVITGSS 330
               L G ++GI+R+    G D      A ++A   L+  G IL +              
Sbjct: 308 ESKPLNGVKVGIIRETLEDGVDSG-VRSATQEAASHLEALGCILTE-------------- 367

Query: 331 SGDWTALLAEFKISLNAYLKELVASPIRSLS--DAIEFNKK--NSKLEKLRE------YG 390
                  L  F + L AY     +    +LS  D + +  +    +L KL E      +G
Sbjct: 368 -----VSLPSFSLGLPAYYVIASSESSSNLSRYDGVRYGNQVMAEELNKLYECSRGEGFG 427

Query: 391 QELFLKA-----EATKGIGDAEKAALARLAKLSKNGFQRVMIKNK-LDAIAAP------- 450
            E+ ++        + G  DA      ++  L +  F+  + +N  L + AAP       
Sbjct: 428 GEVKMRILMGTYALSAGYYDAYYKRAQQVRTLIRKDFKAALEQNDILISPAAPSAAYKIG 487

Query: 451 -----------GRLISPVLAIGGFPGVSVPAGY---NPQGVPFGIGFGGLKGFEPRLIEI 483
                      G +++  + + G P + +P G     P G+P G+   G    E +L+++
Sbjct: 488 EKKDDPLAMYAGDIMTVNVNLAGLPAMVLPCGLVEGGPSGLPVGLQMIGAAFDEEKLLKV 532

BLAST of Lag0009284 vs. TAIR 10
Match: AT5G64440.1 (fatty acid amide hydrolase )

HSP 1 Score: 108.6 bits (270), Expect = 1.4e-23
Identity = 121/447 (27.07%), Postives = 186/447 (41.61%), Query Frame = 0

Query: 50  DALDQASKADYERRRNALGS-LSPLHGIPVLLKDNIATRDKLNTTAGSFALLGSIVPRDA 109
           DA +   +A+   RR   G+ +S L GI V +KD+I           ++      V +D+
Sbjct: 173 DANEVIKQAEASTRRFEQGNPISVLDGIFVTIKDDIDCLPHPTNGGTTWLHEDRSVEKDS 232

Query: 110 GVVTKLRKAGAIIFGKASLSEWSNFRSYEPPNGWSARGGHGKNPYTLGEPCGSSSGSAIS 169
            VV+KLR  GAI+ GKA++ E     +    N  + R  H    YT     GSSSGSA  
Sbjct: 233 AVVSKLRSCGAILLGKANMHELGMGTTGNNSNYGTTRNPHDPKRYT----GGSSSGSAAI 292

Query: 170 VAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISPRQDTVGPICRTVS 229
           VAA + + +LGT+  GS+  PS L  + G+K T G T   G +      + +GP+  ++ 
Sbjct: 293 VAAGLCSAALGTDGGGSVRIPSALCGITGLKTTYGRTDMTGSLCEGGTVEIIGPLASSLE 352

Query: 230 DAAYVLDAIVGADSYDNSTIEAS-----KYIPRGGYGQFLRADGLQGKRIGIVRKFFYFG 289
           DA  V  AI+G+ S D   ++ S     K +   G      ++ +   R+G   K+F   
Sbjct: 353 DAFLVYAAILGSSSADRYNLKPSPPCFPKLLSHNG------SNAIGSLRLGKYTKWFNDV 412

Query: 290 HDDAFYPRAFEKAFKTLKQGGAILVDNLTIDSL------HVIT-GSSS-GDWTALLAEFK 349
                  +  E   K L       V  + +  L      HVI+ GS +    T      K
Sbjct: 413 SSSDISDKC-EDILKLLSNNHGCKVVEIVVPELEEMRAAHVISIGSPTLSSLTPYCEAGK 472

Query: 350 ISLNAYLKELVASPIRSLSDAIEFNKKNSKLEKLREYGQELFLKAEATKGIGDAEKAALA 409
            S  +Y      +  RS S A ++        +L EY   +F   +          A + 
Sbjct: 473 NSKLSYDTRTSFAIFRSFS-ASDYIAAQCLRRRLMEYHLNIFKDVDVIVTPTTGMTAPVI 532

Query: 410 RLAKLSKNGFQRVMIKNKLDAIAAPGRLISPVLAIGGFPGVSVPAGYNPQGVPFGIGFGG 469
               L KNG   + +   L        L+       GFP +SVP GY+ +G+P G+   G
Sbjct: 533 PPDAL-KNGETNIQVTTDLMRFVLAANLL-------GFPAISVPVGYDKEGLPIGLQIMG 592

Query: 470 LKGFEPRLIEIAYGFEHLTKSRKPPPL 483
               E  ++ +A   E L    K P +
Sbjct: 593 RPWAEATVLGLAAAVEELAPVTKKPAI 599

BLAST of Lag0009284 vs. TAIR 10
Match: AT5G07360.1 (Amidase family protein )

HSP 1 Score: 100.1 bits (248), Expect = 5.0e-21
Identity = 75/219 (34.25%), Postives = 110/219 (50.23%), Query Frame = 0

Query: 38  VKDLQRVIEVNPDALDQASKADYERRRNALGSLS------PLHGIPVLLKDNIATRDKLN 97
           +K L+R   V    +    +  Y++ + A   LS      PLHGIP  LKD +A      
Sbjct: 227 LKQLKRYNHVLEAVVTYTEELAYKQAKEADDLLSQGTYLGPLHGIPYGLKDIVAV-PGYK 286

Query: 98  TTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSNFRSYEPPNGWSARGGHGKN 157
           TT GS +     +  +A V  +L+ +GA++  K          S    + W   GG  +N
Sbjct: 287 TTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKLVTG------SMAYDDIWF--GGRTRN 346

Query: 158 PYTLGE-PCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 217
           P+ + E   GSS+G A S +A MV  ++G+ET GS+  P+    +  ++PT G   R GV
Sbjct: 347 PWNIEEFSTGSSAGPAASTSAGMVPFAIGSETAGSMTYPAARCGITALRPTFGSVGRTGV 406

Query: 218 VPISPRQDTVGPICRTVSDAAYVLDAIVGADSYDNSTIE 250
           + IS   D +GP CRT +D A +LDAI G D  D S+ E
Sbjct: 407 MSISESLDKLGPFCRTAADCAVILDAIKGKDPDDLSSRE 436

BLAST of Lag0009284 vs. TAIR 10
Match: AT5G07360.2 (Amidase family protein )

HSP 1 Score: 89.7 bits (221), Expect = 6.8e-18
Identity = 73/219 (33.33%), Postives = 106/219 (48.40%), Query Frame = 0

Query: 38  VKDLQRVIEVNPDALDQASKADYERRRNALGSLS------PLHGIPVLLKDNIATRDKLN 97
           +K L+R   V    +    +  Y++ + A   LS      PLHGIP  LKD +A      
Sbjct: 227 LKQLKRYNHVLEAVVTYTEELAYKQAKEADDLLSQGTYLGPLHGIPYGLKDIVAV-PGYK 286

Query: 98  TTAGSFALLGSIVPRDAGVVTKLRKAGAIIFGKASLSEWSNFRSYEPPNGWSARGGHGKN 157
           TT GS +     +  +A V  +L+ +GA++  K          S    + W   GG  +N
Sbjct: 287 TTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKLVTG------SMAYDDIWF--GGRTRN 346

Query: 158 PYTLGE-PCGSSSGSAISVAANMVTVSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGV 217
           P+ + E   GSS+G A S +A       G+ET GS+  P+    +  ++PT G   R GV
Sbjct: 347 PWNIEEFSTGSSAGPAASTSA-------GSETAGSMTYPAARCGITALRPTFGSVGRTGV 406

Query: 218 VPISPRQDTVGPICRTVSDAAYVLDAIVGADSYDNSTIE 250
           + IS   D +GP CRT +D A +LDAI G D  D S+ E
Sbjct: 407 MSISESLDKLGPFCRTAADCAVILDAIKGKDPDDLSSRE 429