Lag0009282 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0009282 vs. NCBI nr
Match: XP_022146229.1 (putative amidase C869.01 [Momordica charantia])

HSP 1 Score: 674.5 bits (1739), Expect = 6.5e-190
Identity = 350/426 (82.16%), Postives = 378/426 (88.73%), Query Frame = 0

Query: 27  NNADRERKVKAPGSRSLLHGIPVLVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTRLR 86
           + ADRERK KAPGS  LLHGIPVLVKDN+ATKDKLNTTAGS ALLGSVVPRDAG V RLR
Sbjct: 86  DKADRERKAKAPGSLPLLHGIPVLVKDNMATKDKLNTTAGSLALLGSVVPRDAGAVMRLR 145

Query: 87  KAGAIILGKASLSEWANFRSYGAPSGWNARAGHGREPYTLGDPCGSSSGSAISVSANMAA 146
           KAGAIILGKAS+SEWA FRS GAP+GWNARAG GREPYTLG PCGSSSGSAISVSANMAA
Sbjct: 146 KAGAIILGKASMSEWAGFRSNGAPTGWNARAGQGREPYTLGLPCGSSSGSAISVSANMAA 205

Query: 147 VTLGTETDGSILCPCSQNSVVGIKPTVGLTSRAGVVPISPRQDTVGPICRTVSDAAYVLD 206
           V LGTETDGSILCP S NSVVGIKPT+GLTS AGVVPISPRQDTVGPICRTVSDAAYVLD
Sbjct: 206 VALGTETDGSILCPSSFNSVVGIKPTLGLTSTAGVVPISPRQDTVGPICRTVSDAAYVLD 265

Query: 207 VIVGTDALDNETYKTSRYIPGGGYGQFLRANGLKGKRLGIVEDLFSVTEPFLIQAFEEIF 266
           VIVGTD LDN TYK SRYIP GGYGQFL+A+GL+GKRLGIVED F V +P LI AFEEIF
Sbjct: 266 VIVGTDPLDNSTYKASRYIPRGGYGQFLKADGLRGKRLGIVEDFFGVVDPSLIPAFEEIF 325

Query: 267 TILRKRGAILVNNLKIDNLGIIY-SAESGEDIALLNEFKVSLNAYLRELISSPVRTLSDV 326
           TIL KRGAILV+NLKIDNLG IY + ESGE  ALLNEFKVSLNAYL+EL+SSP+R+L++ 
Sbjct: 326 TILSKRGAILVDNLKIDNLGAIYNNTESGEVAALLNEFKVSLNAYLKELVSSPIRSLAEA 385

Query: 327 IAFNQKHSKVEKIKEYRQDLFLQAEKTKGIGMEAVLSKLSKLSKDGLEKTITENKLDAIV 386
           IAFNQKHS +EKIKEY QDLFLQAE T G+G+  +LSKL KLSKDGLEKT+ +NKLDAI+
Sbjct: 386 IAFNQKHSSLEKIKEYGQDLFLQAEATNGMGINGLLSKLGKLSKDGLEKTMIKNKLDAIM 445

Query: 387 TPDSSISTLLAIGGFPGITVPAGYLPKLGTPFGIHFGGLKGFEPKLIEIAYDFEQATKRR 446
           TP + IS +LAIGGFPGITVPAGY P  GTPFGI FGGLKG+EPKLIEIAY FEQATKRR
Sbjct: 446 TPSAIISPILAIGGFPGITVPAGYSPS-GTPFGIDFGGLKGYEPKLIEIAYGFEQATKRR 505

Query: 447 KPPPLK 452
           + PPLK
Sbjct: 506 RKPPLK 510

BLAST of Lag0009282 vs. NCBI nr
Match: XP_004147023.1 (probable amidase At4g34880 [Cucumis sativus] >KGN61907.1 hypothetical protein Csa_006678 [Cucumis sativus])

HSP 1 Score: 568.2 bits (1463), Expect = 6.5e-158
Identity = 297/429 (69.23%), Postives = 348/429 (81.12%), Query Frame = 0

Query: 27  NNADRERKVKAPGSRSLLHGIPVLVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTRLR 86
           + AD ERK  +P S S LHGIPVLVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVT+LR
Sbjct: 86  SRADLERKKSSPRSLSPLHGIPVLVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTKLR 145

Query: 87  KAGAIILGKASLSEWANFRSYGAPSGWNARAGHGREPYTLGDPCGSSSGSAISVSANMAA 146
            AGAII GKASLSEW+ FRS   PSGW+AR G G+ PYT+G+PCGSSSGSAISV+ANM  
Sbjct: 146 MAGAIIFGKASLSEWSYFRSNALPSGWSARGGQGKNPYTMGEPCGSSSGSAISVAANMVT 205

Query: 147 VTLGTETDGSILCPCSQNSVVGIKPTVGLTSRAGVVPISPRQDTVGPICRTVSDAAYVLD 206
           V+LGTETDGSILCP + NSVVGIKPTVGLTSRAGVVPIS RQDTVGPICRTVSDAAYVL+
Sbjct: 206 VSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISSRQDTVGPICRTVSDAAYVLE 265

Query: 207 VIVGTDALDNETYKTSRYIPGGGYGQFLRANGLKGKRLGIVEDL--FSVTEPFLIQAFEE 266
            IVG D  DN T + S+YIP GGYGQFLRA GLKGKR+GIV +   F   + F  QA+E+
Sbjct: 266 AIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKGKRIGIVREFYDFGPDDTFYTQAYEK 325

Query: 267 IFTILRKRGAILVNNLKIDNLGIIYSAESGEDIALLNEFKVSLNAYLRELISSPVRTLSD 326
           +   L+K GAILV+NL IDNL  I+   SGE IALL EFK+SLNAYL+EL++SP+R+LSD
Sbjct: 326 VVKTLKKGGAILVDNLMIDNLEQIFDGSSGEQIALLAEFKISLNAYLKELVASPIRSLSD 385

Query: 327 VIAFNQKHSKVEKIKEYRQDLFLQAEKTKGIG--MEAVLSKLSKLSKDGLEKTITENKLD 386
            I FN+K+SK+E + EY Q+ FL+AE T GIG   +A L++L+KLSKDG E+ + +NKLD
Sbjct: 386 AIEFNKKNSKLENL-EYGQEEFLKAEATNGIGDAEKAALARLAKLSKDGFERLMIKNKLD 445

Query: 387 AIVTPDSSISTLLAIGGFPGITVPAGYLPKLGTPFGIHFGGLKGFEPKLIEIAYDFEQAT 446
           A+  P S IS + AIGGFPG++VPAGY P+ G P+GI FGGLKGFEP+LIEIAY FE+ T
Sbjct: 446 AVAAPGSLISPVFAIGGFPGVSVPAGYDPQ-GNPYGICFGGLKGFEPRLIEIAYGFERLT 505

Query: 447 KRRKPPPLK 452
           K RKPP +K
Sbjct: 506 KSRKPPSIK 512

BLAST of Lag0009282 vs. NCBI nr
Match: XP_008457661.1 (PREDICTED: putative amidase C869.01 [Cucumis melo] >ADN34008.1 amidase [Cucumis melo subsp. melo] >KAA0045711.1 putative amidase [Cucumis melo var. makuwa] >TYJ99569.1 putative amidase [Cucumis melo var. makuwa])

HSP 1 Score: 565.1 bits (1455), Expect = 5.5e-157
Identity = 295/428 (68.93%), Postives = 346/428 (80.84%), Query Frame = 0

Query: 27  NNADRERKVKAPGSRSLLHGIPVLVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTRLR 86
           + AD ERK  +P S S LHGIPVLVKDNIATKDKLNTTAGSFALLGS+VPRDAGVVT+LR
Sbjct: 86  SRADIERKKSSPRSLSPLHGIPVLVKDNIATKDKLNTTAGSFALLGSIVPRDAGVVTKLR 145

Query: 87  KAGAIILGKASLSEWANFRSYGAPSGWNARAGHGREPYTLGDPCGSSSGSAISVSANMAA 146
            AGAII GKASLSEW+ FRS   PSGW+AR G G+ PY LG+PCGSSSGSAISV+ANM  
Sbjct: 146 MAGAIIFGKASLSEWSYFRSNELPSGWSARGGQGKNPYILGEPCGSSSGSAISVAANMVT 205

Query: 147 VTLGTETDGSILCPCSQNSVVGIKPTVGLTSRAGVVPISPRQDTVGPICRTVSDAAYVLD 206
           V+LGTETDGSILCP + NSVVGIKPTVGLTSRAGV+PIS RQD+VGPICRTVSDA YVLD
Sbjct: 206 VSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVIPISSRQDSVGPICRTVSDATYVLD 265

Query: 207 VIVGTDALDNETYKTSRYIPGGGYGQFLRANGLKGKRLGIVEDL--FSVTEPFLIQAFEE 266
            IVG D  DN T + S+YIP GGYGQFLRA GLKGKR+GIV +   F   E F  QA+E+
Sbjct: 266 AIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKGKRIGIVREFYDFGHDETFYPQAYEK 325

Query: 267 IFTILRKRGAILVNNLKIDNLGIIYSAESGEDIALLNEFKVSLNAYLRELISSPVRTLSD 326
           +   L+K GAILV+N  IDNL +I+   SGE IALL EFK+SLNAYL+EL++SP+R+LSD
Sbjct: 326 VLKTLKKGGAILVDNRTIDNLQLIFDGSSGEQIALLAEFKISLNAYLKELVASPIRSLSD 385

Query: 327 VIAFNQKHSKVEKIKEYRQDLFLQAEKTKGIG--MEAVLSKLSKLSKDGLEKTITENKLD 386
            I FN+K+SK+E + EY Q+LFL+AE T GIG   +A L++L+KLSKDG E+ + +NKLD
Sbjct: 386 AIEFNKKNSKLENL-EYGQELFLKAEATNGIGDAEKAALARLAKLSKDGFERLMIKNKLD 445

Query: 387 AIVTPDSSISTLLAIGGFPGITVPAGYLPKLGTPFGIHFGGLKGFEPKLIEIAYDFEQAT 446
           AI  P S IS++ AIGGFPG++VPAGY P+ G P+GI FGGLKGFEP+LIEIAY FE  T
Sbjct: 446 AIAAPGSLISSVFAIGGFPGVSVPAGYDPQ-GVPYGITFGGLKGFEPRLIEIAYGFEHLT 505

Query: 447 KRRKPPPL 451
           K RKPP +
Sbjct: 506 KSRKPPSI 511

BLAST of Lag0009282 vs. NCBI nr
Match: XP_038900912.1 (LOW QUALITY PROTEIN: probable amidase At4g34880 [Benincasa hispida])

HSP 1 Score: 564.7 bits (1454), Expect = 7.2e-157
Identity = 290/429 (67.60%), Postives = 349/429 (81.35%), Query Frame = 0

Query: 27  NNADRERKVKAPGSRSLLHGIPVLVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTRLR 86
           + AD ERK  +P S S LHGIPVLVKDNIAT+DKLNTTAGSFALLGS+VPRDAGVVT+LR
Sbjct: 91  SQADLERKRNSPSSLSPLHGIPVLVKDNIATRDKLNTTAGSFALLGSIVPRDAGVVTKLR 150

Query: 87  KAGAIILGKASLSEWANFRSYGAPSGWNARAGHGREPYTLGDPCGSSSGSAISVSANMAA 146
           KAGAII GKASLS+W+ FRSY  PSGW+AR G G+ PYT+G+PCGSS GSAIS++ANM A
Sbjct: 151 KAGAIIFGKASLSKWSYFRSYXIPSGWSARGGQGKNPYTMGEPCGSSGGSAISIAANMVA 210

Query: 147 VTLGTETDGSILCPCSQNSVVGIKPTVGLTSRAGVVPISPRQDTVGPICRTVSDAAYVLD 206
           V+LGTETDGSILCP   NSVVGIKPTVGLTSRAGV+PIS RQDTVGPICRTVSD AYVL 
Sbjct: 211 VSLGTETDGSILCPSILNSVVGIKPTVGLTSRAGVIPISLRQDTVGPICRTVSDDAYVLG 270

Query: 207 VIVGTDALDNETYKTSRYIPGGGYGQFLRANGLKGKRLGIVEDLFSV--TEPFLIQAFEE 266
            IVG D  DN T + S+YIP GGY QFLRA+GLKGKR+GIV   F     + F  +A+E+
Sbjct: 271 AIVGADKNDNSTIEASKYIPRGGYAQFLRADGLKGKRIGIVRKFFDFGHDDAFYPRAYEK 330

Query: 267 IFTILRKRGAILVNNLKIDNLGIIYSAESGEDIALLNEFKVSLNAYLRELISSPVRTLSD 326
           +F  LR+ GA+ V+NL ID+L +I  + SGE+ ALL EFK+SLNAYL++L++SP+R+LSD
Sbjct: 331 VFKTLRQGGAVFVDNLTIDSLQVIMGSSSGEETALLAEFKISLNAYLKQLVASPIRSLSD 390

Query: 327 VIAFNQKHSKVEKIKEYRQDLFLQAEKTKGIG--MEAVLSKLSKLSKDGLEKTITENKLD 386
            I FN+K+SK+EK++EY Q+LF++AE TKGIG   +A L++L+KLSKDG E+ + +NKLD
Sbjct: 391 AIEFNEKNSKLEKLREYGQNLFIEAEATKGIGDAEKAALARLAKLSKDGFERVMLKNKLD 450

Query: 387 AIVTPDSSISTLLAIGGFPGITVPAGYLPKLGTPFGIHFGGLKGFEPKLIEIAYDFEQAT 446
           AI  P   IS++LAIGGFPGI+VPAGY  + G PFGI FGGLKGFEP+LIEIAY FE  T
Sbjct: 451 AIAAPGWEISSVLAIGGFPGISVPAGYNSQ-GVPFGISFGGLKGFEPRLIEIAYGFEHLT 510

Query: 447 KRRKPPPLK 452
           K RK P +K
Sbjct: 511 KSRKSPSIK 518

BLAST of Lag0009282 vs. NCBI nr
Match: KAF3953674.1 (hypothetical protein CMV_020907 [Castanea mollissima])

HSP 1 Score: 563.1 bits (1450), Expect = 2.1e-156
Identity = 298/428 (69.63%), Postives = 348/428 (81.31%), Query Frame = 0

Query: 27  NNADRERKVKAPGSRSLLHGIPVLVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTRLR 86
           + AD ERK K PGS+S LHGIP+L+KDNIATKDKLNTTAGSFALLGSVVPRDAGVV++LR
Sbjct: 379 DKADYERKAKVPGSQSRLHGIPILLKDNIATKDKLNTTAGSFALLGSVVPRDAGVVSKLR 438

Query: 87  KAGAIILGKASLSEWANFRSYGAPSGWNARAGHGREPYTLGDPCGSSSGSAISVSANMAA 146
           KAGAIILGKASL EW++FR+   P  W+AR G G+ PYTLG+PCGSSSGS+ISV+ANM A
Sbjct: 439 KAGAIILGKASLGEWSHFRTARGPLAWSARGGQGKNPYTLGEPCGSSSGSSISVAANMVA 498

Query: 147 VTLGTETDGSILCPCSQNSVVGIKPTVGLTSRAGVVPISPRQDTVGPICRTVSDAAYVLD 206
           V+LGTETDGSI+CP S NSVVGIKPTVGLTSRAGV+PISPRQDTVGPICRTVSDA +VLD
Sbjct: 499 VSLGTETDGSIICPASFNSVVGIKPTVGLTSRAGVIPISPRQDTVGPICRTVSDAVHVLD 558

Query: 207 VIVGTDALDNETYKTSRYIPGGGYGQFLRANGLKGKRLGIVEDLF--SVTEPFLIQAFEE 266
            I G D  DN T+K S YIP GGYGQFL+ +GL+GKRLGIV + F       +L Q FE+
Sbjct: 559 AIAGIDNNDNATFKASSYIPSGGYGQFLKVDGLRGKRLGIVRNPFFDFGNNTYLNQTFEQ 618

Query: 267 IFTILRKRGAILVNNLKIDNLGII--YSAESGEDIALLNEFKVSLNAYLRELISSPVRTL 326
            F  LR+RGAILV++L+I N+G+I  Y A SGE+ ALL EFK+SLNAYL+EL+SSPVRT+
Sbjct: 619 HFDTLRQRGAILVDHLEITNIGMILDYKA-SGEETALLAEFKISLNAYLKELVSSPVRTM 678

Query: 327 SDVIAFNQKHSKVEKIKEYRQDLFLQAEKTKGIGM--EAVLSKLSKLSKDGLEKTITENK 386
           +DVIAFN K+ ++E I E  QD FL AE TKGIG   +A LS L++LSKDG EK +T NK
Sbjct: 679 ADVIAFNNKNPELEMINEIDQDHFLSAEATKGIGKVEKAALSNLARLSKDGFEKLMTNNK 738

Query: 387 LDAIVTPDSSISTLLAIGGFPGITVPAGYLPKLGTPFGIHFGGLKGFEPKLIEIAYDFEQ 446
           LDA+VTP    S++LAIGGFPG++VPAGY    G PFGI FGGLKG EPKLIEIAY FEQ
Sbjct: 739 LDALVTPSYYASSVLAIGGFPGVSVPAGY-DTTGEPFGICFGGLKGSEPKLIEIAYGFEQ 798

Query: 447 ATKRRKPP 449
           A K R+PP
Sbjct: 799 AKKIRRPP 804

BLAST of Lag0009282 vs. ExPASy Swiss-Prot
Match: A0A1P8B760 (Probable amidase At4g34880 OS=Arabidopsis thaliana OX=3702 GN=At4g34880 PE=2 SV=1)

HSP 1 Score: 493.8 bits (1270), Expect = 2.0e-138
Identity = 274/457 (59.96%), Postives = 340/457 (74.40%), Query Frame = 0

Query: 5   ITFYTSKLAKSNYLPTKFALIFNN---------ADRERKVKAPGSRSLLHGIPVLVKDNI 64
           +  Y   ++K N  P   A+I  N         ADRER +K      +LHG+PVL+KD+I
Sbjct: 63  VELYLEAISKLN--PILHAVIETNPDALIQAEIADRERDLKNTTKLPILHGVPVLLKDSI 122

Query: 65  ATKDKLNTTAGSFALLGSVVPRDAGVVTRLRKAGAIILGKASLSEWANFRSYGAPSGWNA 124
           +TKDKLNTTAGSFALLGSVV RDAGVV RLR++GA+ILGKASLSEWA+FRS+  P GW+A
Sbjct: 123 STKDKLNTTAGSFALLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFSIPDGWSA 182

Query: 125 RAGHGREPYTL-GDPCGSSSGSAISVSANMAAVTLGTETDGSILCPCSQNSVVGIKPTVG 184
           R   G+ PY L  +P GSSSGSAISV+AN+ AV+LGTETDGSIL P SQNSVVGIKP+VG
Sbjct: 183 RGLQGKNPYVLSANPSGSSSGSAISVTANLVAVSLGTETDGSILSPASQNSVVGIKPSVG 242

Query: 185 LTSRAGVVPISPRQDTVGPICRTVSDAAYVLDVIVGTDALDNETYKTSRYIPGGGYGQFL 244
           LTSRAGVVPIS RQD++GPICRTVSDA ++LD IVG D LD  T   S +IP GGY QFL
Sbjct: 243 LTSRAGVVPISLRQDSIGPICRTVSDAVHLLDAIVGYDPLDEATKTASEFIPEGGYKQFL 302

Query: 245 RANGLKGKRLGIVEDLFSVTEPFLIQAFEEIFTILRKRGAILVNNLKIDNLGIIY-SAES 304
             +GLKGKRLGIV    S+ +  +          LR+ GAI++NNL I N+ +I    +S
Sbjct: 303 TTSGLKGKRLGIVMKHSSLLDHHI--------KTLRREGAIVINNLTIPNIEVIVGGTDS 362

Query: 305 GEDIALLNEFKVSLNAYLRELISSPVRTLSDVIAFNQKHSKVEKIKEYRQDLFLQAEKTK 364
           GE+IALL EFK+SLNAYL+EL+ SPVR+L+DVIA+N++ ++ EK+KE+ Q++FL AE T 
Sbjct: 363 GEEIALLAEFKMSLNAYLKELVKSPVRSLADVIAYNEEFAEQEKVKEWGQEVFLTAEATS 422

Query: 365 GIG--MEAVLSKLSKLSKDGLEKTITENKLDAIVTPDSSISTLLAIGGFPGITVPAGYLP 424
           G+G   +  L K+ +LS++G+EK I ENKLDAIVT  S +S++LAIGG+PGI VPAGY  
Sbjct: 423 GMGEKEKTALQKMKELSRNGIEKLIEENKLDAIVTLGSDLSSVLAIGGYPGINVPAGY-D 482

Query: 425 KLGTPFGIHFGGLKGFEPKLIEIAYDFEQATKRRKPP 449
             G P+GI FGGL+  EPKLIEIA+ FEQAT  RKPP
Sbjct: 483 SGGVPYGISFGGLRFSEPKLIEIAFAFEQATLIRKPP 508

BLAST of Lag0009282 vs. ExPASy Swiss-Prot
Match: D4B3C8 (Putative amidase ARB_02965 OS=Arthroderma benhamiae (strain ATCC MYA-4681 / CBS 112371) OX=663331 GN=ARB_02965 PE=1 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 8.8e-65
Identity = 174/457 (38.07%), Postives = 247/457 (54.05%), Query Frame = 0

Query: 25  IFNNADRERKVKAPGSRSLLHGIPVLVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTR 84
           I    D ERK+     R  LHG+P+++K+NI T DK+++TAGS+A+ G+    DA V T+
Sbjct: 136 IAKQMDNERKMGK--LRGPLHGLPIVIKNNIFTDDKMSSTAGSYAIFGARTSADATVATK 195

Query: 85  LRKAGAIILGKASLSEWANFRSYGAPSGWNARAGHGREPYTLG-DPCGSSSGSAISVSAN 144
           LR+AG +I+GK+  S+WANFRS  + +GW+A  G     Y    DP GSSSGS ++    
Sbjct: 196 LREAGLVIMGKSGASQWANFRSLNSTNGWSAYGGQVTAAYIKNQDPSGSSSGSGVASDLG 255

Query: 145 MAAVTLGTETDGSILCPCSQNSVVGIKPTVGLTSRAGVVPISPRQDTVGPICRTVSDAAY 204
           +A  TLGTET GSI+ P  ++++VG+KPTVGLTSR  VVPIS RQDTVGP+ R+V DAAY
Sbjct: 256 LAFATLGTETSGSIVSPADKSNIVGLKPTVGLTSRRFVVPISERQDTVGPMARSVKDAAY 315

Query: 205 VLDVIVGTDALDNETYKTSRYIPGGGYGQFLRA---NGLKGKRLGIVEDLFSV--TEPFL 264
           +L VI G D+ DN T      IP      +++A   N LKGKR+G+  ++  +  +   +
Sbjct: 316 LLQVIAGKDSNDNYT----SAIPFDTIPDYVKACDINALKGKRIGVPRNVIKIFGSPQTV 375

Query: 265 IQAFEEIFTILRKRGAILVNNLKIDNLGIIYSAESGEDIALLNEFKVSLNAYLRELISSP 324
           +  F +   +++K GAI+V N    +      +   +DI L  +   +L A+ ++L  +P
Sbjct: 376 VDQFNQALAVMKKAGAIIVENTDFTSFAEFAQSPIPDDI-LYADSLTNLPAFFKQLKVNP 435

Query: 325 --VRTLSDVIAFNQKHSKVEKIKEY------RQDLFLQ-AEKTKGIGMEAVLSKLSKLSK 384
             +  L  +  F Q H    +++EY      R D+ LQ   K        +  K  K   
Sbjct: 436 HNITDLESLRRFTQHH----RLEEYPSRDTARWDIALQKGIKNTDPKFWPMYQKNVKFGN 495

Query: 385 D-GLEKTITENKLDAIVTPDSSISTLLAIGGFPGITVPAGYLPK--------------LG 444
           + G+   +  +KLDA V P      + A+ G P ITVP G  P                G
Sbjct: 496 EGGILGALRRHKLDAAVLPTDLSPYIPALIGSPIITVPMGVYPNGTKVNHDRELVTSGPG 555

Query: 445 TPFGIHFGGLKGFEPKLIEIAYDFEQATKRRKPPPLK 452
            P GI F G    E KLI +AY FEQ T  R  P LK
Sbjct: 556 IPIGIGFMGDLWSEEKLIGLAYAFEQKTHAR--PKLK 579

BLAST of Lag0009282 vs. ExPASy Swiss-Prot
Match: Q9URY4 (Putative amidase C869.01 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAC869.01 PE=3 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 3.3e-64
Identity = 176/445 (39.55%), Postives = 247/445 (55.51%), Query Frame = 0

Query: 41  RSLLHGIPVLVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTRLRKAGAIILGKASLSE 100
           R  LHGIP +VKDN ATKDK++TTAGS+ALLGS+VPRDA VV +LR+AGA++ G A+LSE
Sbjct: 130 RGPLHGIPFIVKDNFATKDKMDTTAGSYALLGSIVPRDAYVVKQLREAGAVLFGHATLSE 189

Query: 101 WANFRSYGAPSGWNARAGHGREPYTLG-DPCGSSSGSAISVSANMAAVTLGTETDGSILC 160
           WA+ RS     G++AR G  R P+ L  +P GSSSGSAISV++NM A  LGTETDGSI+ 
Sbjct: 190 WADMRSNDYSEGYSARGGQSRCPFNLTVNPGGSSSGSAISVASNMIAFALGTETDGSIID 249

Query: 161 PCSQNSVVGIKPTVGLTSRAGVVPISPRQDTVGPICRTVSDAAYVLDVIVGTDALD---- 220
           P  +N VVG+KPTVGLTSR GV+P S  QDT GPI RTV DA YV   + G D  D    
Sbjct: 250 PAMRNGVVGLKPTVGLTSRYGVIPESEHQDTTGPIARTVRDAVYVFQSMWGIDENDIYTL 309

Query: 221 NETYKTSRYIPGGGYGQFL-RANGLKGKRLGIV-EDLFSVTEPFLIQAFEEIFTILRKRG 280
           N+T KT      G Y +FL     L+G R G+  + L+   +   I    E+   + + G
Sbjct: 310 NQTGKTPE---DGDYMKFLSNKTSLEGARFGLPWKRLWQNAKADEIDRLLEVVKQIEEAG 369

Query: 281 AILVNNLKIDNLGIIYSAESGEDIALLNE---------FKVSLNAYLRELISSPVRTLSD 340
           AI+ NN    NL +I +     ++  +NE         F  ++ +YL E+ ++ + +L D
Sbjct: 370 AIVYNNTNFYNLDVISNDGWNWELGSVNESEYTVVKVDFYNNIKSYLSEVKNTEIHSLED 429

Query: 341 VIAFNQKHSKVEKIK-------EYRQDLFLQAEKTKGIGMEA---VLSKLSKLSKD-GLE 400
           ++ +N K+   E  K          QD FL + +  G+  E     +  + + S+D G++
Sbjct: 430 IVEYNNKYMGTEGGKPNIVPAFSSGQDGFLASLEWGGVKNETYWQAVEYVRRTSQDEGID 489

Query: 401 --------KTITENKLDAIVTPDSSISTL--LAIGGFPGITVPAGYLPKLGTPFGIHFGG 449
                   KT     L+ ++ P  +  T    A  G+P IT+P G +   G PFG+    
Sbjct: 490 YALNYTDPKTNDSFILNGLLVPSGTSITYQQAAKAGYPMITLPIG-VKTNGRPFGLGIMH 549

BLAST of Lag0009282 vs. ExPASy Swiss-Prot
Match: B0KBN4 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter pseudethanolicus (strain ATCC 33223 / 39E) OX=340099 GN=gatA PE=3 SV=1)

HSP 1 Score: 147.9 bits (372), Expect = 2.8e-34
Identity = 140/452 (30.97%), Postives = 214/452 (47.35%), Query Frame = 0

Query: 30  DRERKVKAPGSRSLLHGIPVLVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTRLRKAG 89
           + + K+K  G  + L GIPV++KDNI+T + + TT  S  L   + P +A VV +L + G
Sbjct: 57  EADEKIK-NGEDTALTGIPVIIKDNIST-EGIKTTCSSKMLENYIPPYNATVVEKLLEEG 116

Query: 90  AIILGKASLSEWANFRSYGAPSGWNARAGHGREPYTLGD-PCGSSSGSAISVSANMAAVT 149
            IILGK++L E+A        S  N+     + P+ L   P GSS GSA +++A+ AA  
Sbjct: 117 VIILGKSNLDEFAM-----GSSTENSAFKTTKNPWDLSRVPGGSSGGSAAAIAADEAAFA 176

Query: 150 LGTETDGSILCPCSQNSVVGIKPTVGLTSRAGVVPISPRQDTVGPICRTVSDAAYVLDVI 209
           LG++T GSI  P S   VVG+KPT GL SR G+V  +   D +GP  + V+D A VL+ I
Sbjct: 177 LGSDTGGSIRQPASLCGVVGMKPTYGLVSRYGLVAFASSLDQIGPFTKDVTDCAIVLNTI 236

Query: 210 VGTDALDNETYKTSRYIPGGGYGQFLRANGLKGKRLGIVEDLFSV-TEPFLIQAFEEIFT 269
           +G D  D+    TS  I    Y  +L+ + +KG R+G+ ++ F    E  + +  +E   
Sbjct: 237 IGHDPKDS----TSLKIDKPDYTSYLKED-IKGLRIGVAKEFFGEGIEEGVKETVQESIK 296

Query: 270 ILRKRGAILVN------NLKIDNLGIIYSAESGEDIALLNEFKVS-LNAYLRELISSPVR 329
           +L+  GA +++         +    II SAE+  ++A  +  +   +     +LI   + 
Sbjct: 297 VLQDLGAEIIDISIPYVEYALPAYYIIASAEASSNLARYDGIRYGHIAGKYEDLIDMYMV 356

Query: 330 TLSDVIAFNQKHSKVEKIKEYRQDLFLQAEKTKGIGMEAVLSKLSK---LSKDGLEKTIT 389
           T S+              KE ++ + L          +A   K  K   L K+  EK   
Sbjct: 357 TRSEGFG-----------KEVKRRIMLGTYALSSGYYDAYYKKALKVRTLIKNDFEKAF- 416

Query: 390 ENKLDAIVTPDS-------------SISTLLA--------IGGFPGITVPAGYLPKLGTP 449
             K D I+ P S              ++  LA        I G PGI++P G     G P
Sbjct: 417 -EKCDVIIGPTSPTVAFKIGERANDPLAMYLADIYTVSVNIAGLPGISIPCGLSD--GLP 476

BLAST of Lag0009282 vs. ExPASy Swiss-Prot
Match: B0K3S3 (Glutamyl-tRNA(Gln) amidotransferase subunit A OS=Thermoanaerobacter sp. (strain X514) OX=399726 GN=gatA PE=3 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 3.6e-34
Identity = 140/452 (30.97%), Postives = 214/452 (47.35%), Query Frame = 0

Query: 30  DRERKVKAPGSRSLLHGIPVLVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTRLRKAG 89
           + + K+K  G  + L GIPV++KDNI+T + + TT  S  L   + P +A VV +L + G
Sbjct: 57  EADEKIK-KGEDTALTGIPVIIKDNIST-EGIKTTCSSKMLENYIPPYNATVVEKLLEEG 116

Query: 90  AIILGKASLSEWANFRSYGAPSGWNARAGHGREPYTLGD-PCGSSSGSAISVSANMAAVT 149
            IILGK++L E+A        S  N+     + P+ L   P GSS GSA +++A+ AA  
Sbjct: 117 VIILGKSNLDEFAM-----GSSTENSAFKTTKNPWDLSRVPGGSSGGSAAAIAADEAAFA 176

Query: 150 LGTETDGSILCPCSQNSVVGIKPTVGLTSRAGVVPISPRQDTVGPICRTVSDAAYVLDVI 209
           LG++T GSI  P S   VVG+KPT GL SR G+V  +   D +GP  + V+D A VL+ I
Sbjct: 177 LGSDTGGSIRQPASLCGVVGMKPTYGLVSRYGLVAFASSLDQIGPFTKDVTDCAIVLNTI 236

Query: 210 VGTDALDNETYKTSRYIPGGGYGQFLRANGLKGKRLGIVEDLFSV-TEPFLIQAFEEIFT 269
           +G D  D+    TS  I    Y  +L+ + +KG R+G+ ++ F    E  + +  +E   
Sbjct: 237 IGHDPKDS----TSLKIDKPDYTSYLKED-IKGLRIGVAKEFFGEGIEEGVKETVQESIK 296

Query: 270 ILRKRGAILVN------NLKIDNLGIIYSAESGEDIALLNEFKVS-LNAYLRELISSPVR 329
           +L+  GA +++         +    II SAE+  ++A  +  +   +     +LI   + 
Sbjct: 297 VLQDLGAEIIDISIPYVEYALPAYYIIASAEASSNLARYDGIRYGHIAEKYEDLIDMYMV 356

Query: 330 TLSDVIAFNQKHSKVEKIKEYRQDLFLQAEKTKGIGMEAVLSKLSK---LSKDGLEKTIT 389
           T S+              KE ++ + L          +A   K  K   L K+  EK   
Sbjct: 357 TRSEGFG-----------KEVKRRIMLGTYALSSGYYDAYYKKALKVRTLIKNDFEKAF- 416

Query: 390 ENKLDAIVTPDS-------------SISTLLA--------IGGFPGITVPAGYLPKLGTP 449
             K D I+ P S              ++  LA        I G PGI++P G     G P
Sbjct: 417 -EKCDVIIGPTSPTVAFKIGERANDPLAMYLADIYTVSVNIAGLPGISIPCGLSD--GLP 476

BLAST of Lag0009282 vs. ExPASy TrEMBL
Match: A0A6J1CXI8 (putative amidase C869.01 OS=Momordica charantia OX=3673 GN=LOC111015494 PE=4 SV=1)

HSP 1 Score: 674.5 bits (1739), Expect = 3.1e-190
Identity = 350/426 (82.16%), Postives = 378/426 (88.73%), Query Frame = 0

Query: 27  NNADRERKVKAPGSRSLLHGIPVLVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTRLR 86
           + ADRERK KAPGS  LLHGIPVLVKDN+ATKDKLNTTAGS ALLGSVVPRDAG V RLR
Sbjct: 86  DKADRERKAKAPGSLPLLHGIPVLVKDNMATKDKLNTTAGSLALLGSVVPRDAGAVMRLR 145

Query: 87  KAGAIILGKASLSEWANFRSYGAPSGWNARAGHGREPYTLGDPCGSSSGSAISVSANMAA 146
           KAGAIILGKAS+SEWA FRS GAP+GWNARAG GREPYTLG PCGSSSGSAISVSANMAA
Sbjct: 146 KAGAIILGKASMSEWAGFRSNGAPTGWNARAGQGREPYTLGLPCGSSSGSAISVSANMAA 205

Query: 147 VTLGTETDGSILCPCSQNSVVGIKPTVGLTSRAGVVPISPRQDTVGPICRTVSDAAYVLD 206
           V LGTETDGSILCP S NSVVGIKPT+GLTS AGVVPISPRQDTVGPICRTVSDAAYVLD
Sbjct: 206 VALGTETDGSILCPSSFNSVVGIKPTLGLTSTAGVVPISPRQDTVGPICRTVSDAAYVLD 265

Query: 207 VIVGTDALDNETYKTSRYIPGGGYGQFLRANGLKGKRLGIVEDLFSVTEPFLIQAFEEIF 266
           VIVGTD LDN TYK SRYIP GGYGQFL+A+GL+GKRLGIVED F V +P LI AFEEIF
Sbjct: 266 VIVGTDPLDNSTYKASRYIPRGGYGQFLKADGLRGKRLGIVEDFFGVVDPSLIPAFEEIF 325

Query: 267 TILRKRGAILVNNLKIDNLGIIY-SAESGEDIALLNEFKVSLNAYLRELISSPVRTLSDV 326
           TIL KRGAILV+NLKIDNLG IY + ESGE  ALLNEFKVSLNAYL+EL+SSP+R+L++ 
Sbjct: 326 TILSKRGAILVDNLKIDNLGAIYNNTESGEVAALLNEFKVSLNAYLKELVSSPIRSLAEA 385

Query: 327 IAFNQKHSKVEKIKEYRQDLFLQAEKTKGIGMEAVLSKLSKLSKDGLEKTITENKLDAIV 386
           IAFNQKHS +EKIKEY QDLFLQAE T G+G+  +LSKL KLSKDGLEKT+ +NKLDAI+
Sbjct: 386 IAFNQKHSSLEKIKEYGQDLFLQAEATNGMGINGLLSKLGKLSKDGLEKTMIKNKLDAIM 445

Query: 387 TPDSSISTLLAIGGFPGITVPAGYLPKLGTPFGIHFGGLKGFEPKLIEIAYDFEQATKRR 446
           TP + IS +LAIGGFPGITVPAGY P  GTPFGI FGGLKG+EPKLIEIAY FEQATKRR
Sbjct: 446 TPSAIISPILAIGGFPGITVPAGYSPS-GTPFGIDFGGLKGYEPKLIEIAYGFEQATKRR 505

Query: 447 KPPPLK 452
           + PPLK
Sbjct: 506 RKPPLK 510

BLAST of Lag0009282 vs. ExPASy TrEMBL
Match: A0A0A0LM97 (Amidase domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G264020 PE=4 SV=1)

HSP 1 Score: 568.2 bits (1463), Expect = 3.2e-158
Identity = 297/429 (69.23%), Postives = 348/429 (81.12%), Query Frame = 0

Query: 27  NNADRERKVKAPGSRSLLHGIPVLVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTRLR 86
           + AD ERK  +P S S LHGIPVLVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVT+LR
Sbjct: 86  SRADLERKKSSPRSLSPLHGIPVLVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTKLR 145

Query: 87  KAGAIILGKASLSEWANFRSYGAPSGWNARAGHGREPYTLGDPCGSSSGSAISVSANMAA 146
            AGAII GKASLSEW+ FRS   PSGW+AR G G+ PYT+G+PCGSSSGSAISV+ANM  
Sbjct: 146 MAGAIIFGKASLSEWSYFRSNALPSGWSARGGQGKNPYTMGEPCGSSSGSAISVAANMVT 205

Query: 147 VTLGTETDGSILCPCSQNSVVGIKPTVGLTSRAGVVPISPRQDTVGPICRTVSDAAYVLD 206
           V+LGTETDGSILCP + NSVVGIKPTVGLTSRAGVVPIS RQDTVGPICRTVSDAAYVL+
Sbjct: 206 VSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVVPISSRQDTVGPICRTVSDAAYVLE 265

Query: 207 VIVGTDALDNETYKTSRYIPGGGYGQFLRANGLKGKRLGIVEDL--FSVTEPFLIQAFEE 266
            IVG D  DN T + S+YIP GGYGQFLRA GLKGKR+GIV +   F   + F  QA+E+
Sbjct: 266 AIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKGKRIGIVREFYDFGPDDTFYTQAYEK 325

Query: 267 IFTILRKRGAILVNNLKIDNLGIIYSAESGEDIALLNEFKVSLNAYLRELISSPVRTLSD 326
           +   L+K GAILV+NL IDNL  I+   SGE IALL EFK+SLNAYL+EL++SP+R+LSD
Sbjct: 326 VVKTLKKGGAILVDNLMIDNLEQIFDGSSGEQIALLAEFKISLNAYLKELVASPIRSLSD 385

Query: 327 VIAFNQKHSKVEKIKEYRQDLFLQAEKTKGIG--MEAVLSKLSKLSKDGLEKTITENKLD 386
            I FN+K+SK+E + EY Q+ FL+AE T GIG   +A L++L+KLSKDG E+ + +NKLD
Sbjct: 386 AIEFNKKNSKLENL-EYGQEEFLKAEATNGIGDAEKAALARLAKLSKDGFERLMIKNKLD 445

Query: 387 AIVTPDSSISTLLAIGGFPGITVPAGYLPKLGTPFGIHFGGLKGFEPKLIEIAYDFEQAT 446
           A+  P S IS + AIGGFPG++VPAGY P+ G P+GI FGGLKGFEP+LIEIAY FE+ T
Sbjct: 446 AVAAPGSLISPVFAIGGFPGVSVPAGYDPQ-GNPYGICFGGLKGFEPRLIEIAYGFERLT 505

Query: 447 KRRKPPPLK 452
           K RKPP +K
Sbjct: 506 KSRKPPSIK 512

BLAST of Lag0009282 vs. ExPASy TrEMBL
Match: A0A5D3BKT1 (Putative amidase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold123G001390 PE=4 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 2.7e-157
Identity = 295/428 (68.93%), Postives = 346/428 (80.84%), Query Frame = 0

Query: 27  NNADRERKVKAPGSRSLLHGIPVLVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTRLR 86
           + AD ERK  +P S S LHGIPVLVKDNIATKDKLNTTAGSFALLGS+VPRDAGVVT+LR
Sbjct: 86  SRADIERKKSSPRSLSPLHGIPVLVKDNIATKDKLNTTAGSFALLGSIVPRDAGVVTKLR 145

Query: 87  KAGAIILGKASLSEWANFRSYGAPSGWNARAGHGREPYTLGDPCGSSSGSAISVSANMAA 146
            AGAII GKASLSEW+ FRS   PSGW+AR G G+ PY LG+PCGSSSGSAISV+ANM  
Sbjct: 146 MAGAIIFGKASLSEWSYFRSNELPSGWSARGGQGKNPYILGEPCGSSSGSAISVAANMVT 205

Query: 147 VTLGTETDGSILCPCSQNSVVGIKPTVGLTSRAGVVPISPRQDTVGPICRTVSDAAYVLD 206
           V+LGTETDGSILCP + NSVVGIKPTVGLTSRAGV+PIS RQD+VGPICRTVSDA YVLD
Sbjct: 206 VSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVIPISSRQDSVGPICRTVSDATYVLD 265

Query: 207 VIVGTDALDNETYKTSRYIPGGGYGQFLRANGLKGKRLGIVEDL--FSVTEPFLIQAFEE 266
            IVG D  DN T + S+YIP GGYGQFLRA GLKGKR+GIV +   F   E F  QA+E+
Sbjct: 266 AIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKGKRIGIVREFYDFGHDETFYPQAYEK 325

Query: 267 IFTILRKRGAILVNNLKIDNLGIIYSAESGEDIALLNEFKVSLNAYLRELISSPVRTLSD 326
           +   L+K GAILV+N  IDNL +I+   SGE IALL EFK+SLNAYL+EL++SP+R+LSD
Sbjct: 326 VLKTLKKGGAILVDNRTIDNLQLIFDGSSGEQIALLAEFKISLNAYLKELVASPIRSLSD 385

Query: 327 VIAFNQKHSKVEKIKEYRQDLFLQAEKTKGIG--MEAVLSKLSKLSKDGLEKTITENKLD 386
            I FN+K+SK+E + EY Q+LFL+AE T GIG   +A L++L+KLSKDG E+ + +NKLD
Sbjct: 386 AIEFNKKNSKLENL-EYGQELFLKAEATNGIGDAEKAALARLAKLSKDGFERLMIKNKLD 445

Query: 387 AIVTPDSSISTLLAIGGFPGITVPAGYLPKLGTPFGIHFGGLKGFEPKLIEIAYDFEQAT 446
           AI  P S IS++ AIGGFPG++VPAGY P+ G P+GI FGGLKGFEP+LIEIAY FE  T
Sbjct: 446 AIAAPGSLISSVFAIGGFPGVSVPAGYDPQ-GVPYGITFGGLKGFEPRLIEIAYGFEHLT 505

Query: 447 KRRKPPPL 451
           K RKPP +
Sbjct: 506 KSRKPPSI 511

BLAST of Lag0009282 vs. ExPASy TrEMBL
Match: E5GC09 (Amidase OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 2.7e-157
Identity = 295/428 (68.93%), Postives = 346/428 (80.84%), Query Frame = 0

Query: 27  NNADRERKVKAPGSRSLLHGIPVLVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTRLR 86
           + AD ERK  +P S S LHGIPVLVKDNIATKDKLNTTAGSFALLGS+VPRDAGVVT+LR
Sbjct: 86  SRADIERKKSSPRSLSPLHGIPVLVKDNIATKDKLNTTAGSFALLGSIVPRDAGVVTKLR 145

Query: 87  KAGAIILGKASLSEWANFRSYGAPSGWNARAGHGREPYTLGDPCGSSSGSAISVSANMAA 146
            AGAII GKASLSEW+ FRS   PSGW+AR G G+ PY LG+PCGSSSGSAISV+ANM  
Sbjct: 146 MAGAIIFGKASLSEWSYFRSNELPSGWSARGGQGKNPYILGEPCGSSSGSAISVAANMVT 205

Query: 147 VTLGTETDGSILCPCSQNSVVGIKPTVGLTSRAGVVPISPRQDTVGPICRTVSDAAYVLD 206
           V+LGTETDGSILCP + NSVVGIKPTVGLTSRAGV+PIS RQD+VGPICRTVSDA YVLD
Sbjct: 206 VSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVIPISSRQDSVGPICRTVSDATYVLD 265

Query: 207 VIVGTDALDNETYKTSRYIPGGGYGQFLRANGLKGKRLGIVEDL--FSVTEPFLIQAFEE 266
            IVG D  DN T + S+YIP GGYGQFLRA GLKGKR+GIV +   F   E F  QA+E+
Sbjct: 266 AIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKGKRIGIVREFYDFGHDETFYPQAYEK 325

Query: 267 IFTILRKRGAILVNNLKIDNLGIIYSAESGEDIALLNEFKVSLNAYLRELISSPVRTLSD 326
           +   L+K GAILV+N  IDNL +I+   SGE IALL EFK+SLNAYL+EL++SP+R+LSD
Sbjct: 326 VLKTLKKGGAILVDNRTIDNLQLIFDGSSGEQIALLAEFKISLNAYLKELVASPIRSLSD 385

Query: 327 VIAFNQKHSKVEKIKEYRQDLFLQAEKTKGIG--MEAVLSKLSKLSKDGLEKTITENKLD 386
            I FN+K+SK+E + EY Q+LFL+AE T GIG   +A L++L+KLSKDG E+ + +NKLD
Sbjct: 386 AIEFNKKNSKLENL-EYGQELFLKAEATNGIGDAEKAALARLAKLSKDGFERLMIKNKLD 445

Query: 387 AIVTPDSSISTLLAIGGFPGITVPAGYLPKLGTPFGIHFGGLKGFEPKLIEIAYDFEQAT 446
           AI  P S IS++ AIGGFPG++VPAGY P+ G P+GI FGGLKGFEP+LIEIAY FE  T
Sbjct: 446 AIAAPGSLISSVFAIGGFPGVSVPAGYDPQ-GVPYGITFGGLKGFEPRLIEIAYGFEHLT 505

Query: 447 KRRKPPPL 451
           K RKPP +
Sbjct: 506 KSRKPPSI 511

BLAST of Lag0009282 vs. ExPASy TrEMBL
Match: A0A1S3C5K8 (putative amidase C869.01 OS=Cucumis melo OX=3656 GN=LOC103497307 PE=4 SV=1)

HSP 1 Score: 565.1 bits (1455), Expect = 2.7e-157
Identity = 295/428 (68.93%), Postives = 346/428 (80.84%), Query Frame = 0

Query: 27  NNADRERKVKAPGSRSLLHGIPVLVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTRLR 86
           + AD ERK  +P S S LHGIPVLVKDNIATKDKLNTTAGSFALLGS+VPRDAGVVT+LR
Sbjct: 86  SRADIERKKSSPRSLSPLHGIPVLVKDNIATKDKLNTTAGSFALLGSIVPRDAGVVTKLR 145

Query: 87  KAGAIILGKASLSEWANFRSYGAPSGWNARAGHGREPYTLGDPCGSSSGSAISVSANMAA 146
            AGAII GKASLSEW+ FRS   PSGW+AR G G+ PY LG+PCGSSSGSAISV+ANM  
Sbjct: 146 MAGAIIFGKASLSEWSYFRSNELPSGWSARGGQGKNPYILGEPCGSSSGSAISVAANMVT 205

Query: 147 VTLGTETDGSILCPCSQNSVVGIKPTVGLTSRAGVVPISPRQDTVGPICRTVSDAAYVLD 206
           V+LGTETDGSILCP + NSVVGIKPTVGLTSRAGV+PIS RQD+VGPICRTVSDA YVLD
Sbjct: 206 VSLGTETDGSILCPSTLNSVVGIKPTVGLTSRAGVIPISSRQDSVGPICRTVSDATYVLD 265

Query: 207 VIVGTDALDNETYKTSRYIPGGGYGQFLRANGLKGKRLGIVEDL--FSVTEPFLIQAFEE 266
            IVG D  DN T + S+YIP GGYGQFLRA GLKGKR+GIV +   F   E F  QA+E+
Sbjct: 266 AIVGADRYDNSTIEASKYIPKGGYGQFLRAGGLKGKRIGIVREFYDFGHDETFYPQAYEK 325

Query: 267 IFTILRKRGAILVNNLKIDNLGIIYSAESGEDIALLNEFKVSLNAYLRELISSPVRTLSD 326
           +   L+K GAILV+N  IDNL +I+   SGE IALL EFK+SLNAYL+EL++SP+R+LSD
Sbjct: 326 VLKTLKKGGAILVDNRTIDNLQLIFDGSSGEQIALLAEFKISLNAYLKELVASPIRSLSD 385

Query: 327 VIAFNQKHSKVEKIKEYRQDLFLQAEKTKGIG--MEAVLSKLSKLSKDGLEKTITENKLD 386
            I FN+K+SK+E + EY Q+LFL+AE T GIG   +A L++L+KLSKDG E+ + +NKLD
Sbjct: 386 AIEFNKKNSKLENL-EYGQELFLKAEATNGIGDAEKAALARLAKLSKDGFERLMIKNKLD 445

Query: 387 AIVTPDSSISTLLAIGGFPGITVPAGYLPKLGTPFGIHFGGLKGFEPKLIEIAYDFEQAT 446
           AI  P S IS++ AIGGFPG++VPAGY P+ G P+GI FGGLKGFEP+LIEIAY FE  T
Sbjct: 446 AIAAPGSLISSVFAIGGFPGVSVPAGYDPQ-GVPYGITFGGLKGFEPRLIEIAYGFEHLT 505

Query: 447 KRRKPPPL 451
           K RKPP +
Sbjct: 506 KSRKPPSI 511

BLAST of Lag0009282 vs. TAIR 10
Match: AT4G34880.1 (Amidase family protein )

HSP 1 Score: 420.6 bits (1080), Expect = 1.6e-117
Identity = 243/456 (53.29%), Postives = 304/456 (66.67%), Query Frame = 0

Query: 5   ITFYTSKLAKSNYLPTKFALIFNN---------ADRERKVKAPGSRSLLHGIPVLVKDNI 64
           +  Y   ++K N  P   A+I  N         ADRER +K      +LHG+PVL+KD+I
Sbjct: 63  VELYLEAISKLN--PILHAVIETNPDALIQAEIADRERDLKNTTKLPILHGVPVLLKDSI 122

Query: 65  ATKDKLNTTAGSFALLGSVVPRDAGVVTRLRKAGAIILGKASLSEWANFRSYGAPSGWNA 124
           +TKDKLNTTAGSFALLGSVV RDAGVV RLR++GA+ILGKASLSEWA+FRS+  P GW+A
Sbjct: 123 STKDKLNTTAGSFALLGSVVARDAGVVKRLRESGAVILGKASLSEWAHFRSFSIPDGWSA 182

Query: 125 RAGHGREPYTLGDPCGSSSGSAISVSANMAAVTLGTETDGSILCPCSQNSVVGIKPTVGL 184
                                                         SQNSVVGIKP+VGL
Sbjct: 183 P---------------------------------------------SQNSVVGIKPSVGL 242

Query: 185 TSRAGVVPISPRQDTVGPICRTVSDAAYVLDVIVGTDALDNETYKTSRYIPGGGYGQFLR 244
           TSRAGVVPIS RQD++GPICRTVSDA ++LD IVG D LD  T   S +IP GGY QFL 
Sbjct: 243 TSRAGVVPISLRQDSIGPICRTVSDAVHLLDAIVGYDPLDEATKTASEFIPEGGYKQFLT 302

Query: 245 ANGLKGKRLGIVEDLFSVTEPFLIQAFEEIFTILRKRGAILVNNLKIDNLGIIY-SAESG 304
            +GLKGKRLGIV    S+ +  +          LR+ GAI++NNL I N+ +I    +SG
Sbjct: 303 TSGLKGKRLGIVMKHSSLLDHHI--------KTLRREGAIVINNLTIPNIEVIVGGTDSG 362

Query: 305 EDIALLNEFKVSLNAYLRELISSPVRTLSDVIAFNQKHSKVEKIKEYRQDLFLQAEKTKG 364
           E+IALL EFK+SLNAYL+EL+ SPVR+L+DVIA+N++ ++ EK+KE+ Q++FL AE T G
Sbjct: 363 EEIALLAEFKMSLNAYLKELVKSPVRSLADVIAYNEEFAEQEKVKEWGQEVFLTAEATSG 422

Query: 365 IG--MEAVLSKLSKLSKDGLEKTITENKLDAIVTPDSSISTLLAIGGFPGITVPAGYLPK 424
           +G   +  L K+ +LS++G+EK I ENKLDAIVT  S +S++LAIGG+PGI VPAGY   
Sbjct: 423 MGEKEKTALQKMKELSRNGIEKLIEENKLDAIVTLGSDLSSVLAIGGYPGINVPAGY-DS 462

Query: 425 LGTPFGIHFGGLKGFEPKLIEIAYDFEQATKRRKPP 449
            G P+GI FGGL+  EPKLIEIA+ FEQAT  RKPP
Sbjct: 483 GGVPYGISFGGLRFSEPKLIEIAFAFEQATLIRKPP 462

BLAST of Lag0009282 vs. TAIR 10
Match: AT3G25660.1 (Amidase family protein )

HSP 1 Score: 97.8 bits (242), Expect = 2.3e-20
Identity = 121/438 (27.63%), Postives = 193/438 (44.06%), Query Frame = 0

Query: 44  LHGIPVLVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTRLRKAGAIILGKASLSEWAN 103
           L G+ + VKDNI T+  + +TA S  L     P DA  V ++++ G I++GK ++ E   
Sbjct: 108 LAGVLIGVKDNICTQG-MPSTAASRILEHYRPPFDATAVKKIKELGGIVVGKTNMDE--- 167

Query: 104 FRSYGAPSGWNARAGH-GREPYTLGD-PCGSSSGSAISVSANMAAVTLGTETDGSILCPC 163
              +G  S   A A      P+ L   P GSS GSA +V+A    V+LG++T GS+  P 
Sbjct: 168 ---FGMGSTTEASAFQVTANPWDLSRVPGGSSGGSAAAVAARQCMVSLGSDTGGSVRQPA 227

Query: 164 SQNSVVGIKPTVGLTSRAGVVPISPRQDTVGPICRTVSDAAYVLDVIVGTDALDNETYKT 223
           S   VVG+KPT G  SR G++  +   D +G    TV+DA  +L  I G D  D+ + K 
Sbjct: 228 SFCGVVGLKPTYGRVSRFGLMAYASSLDVIGCFGSTVADAGMLLHAISGYDRFDSTSSKQ 287

Query: 224 SRYIPGGGYGQFL-----RANGLKGKRLGIV-EDLFSVTEPFLIQAFEEIFTILRKRGAI 283
              +P     QFL      +  L G ++GI+ E L    +  +  A +E  + L   G I
Sbjct: 288 D--VP-EFQSQFLSVDHFESKPLNGVKVGIIRETLEDGVDSGVRSATQEAASHLEALGCI 347

Query: 284 LVN-NLKIDNLG-----IIYSAESGEDIALLNEFKVSLNAYLRELI-------------S 343
           L   +L   +LG     +I S+ES  +++  +  +        EL               
Sbjct: 348 LTEVSLPSFSLGLPAYYVIASSESSSNLSRYDGVRYGNQVMAEELNKLYECSRGEGFGGE 407

Query: 344 SPVRTLSDVIAFNQKHSKVEKIKEYRQDLFLQAEKTKGIGMEAVLSKLSKLSKDGLEKTI 403
             +R L    A +  +      +  +    ++ +    +    +L  +S  +     K I
Sbjct: 408 VKMRILMGTYALSAGYYDAYYKRAQQVRTLIRKDFKAALEQNDIL--ISPAAPSAAYK-I 467

Query: 404 TENKLDAIVTPDSSISTL-LAIGGFPGITVPAGYLP--KLGTPFGIHFGGLKGFEPKLIE 451
            E K D +      I T+ + + G P + +P G +     G P G+   G    E KL++
Sbjct: 468 GEKKDDPLAMYAGDIMTVNVNLAGLPAMVLPCGLVEGGPSGLPVGLQMIGAAFDEEKLLK 527

BLAST of Lag0009282 vs. TAIR 10
Match: AT5G64440.1 (fatty acid amide hydrolase )

HSP 1 Score: 90.9 bits (224), Expect = 2.8e-18
Identity = 123/465 (26.45%), Postives = 195/465 (41.94%), Query Frame = 0

Query: 19  PTKFALIFNNADRERKVKAPGSR-------SLLHGIPVLVKDNIATKDKLNTTAGSFALL 78
           PT F + F+  +  ++ +A   R       S+L GI V +KD+I           ++   
Sbjct: 165 PTPFLIRFDANEVIKQAEASTRRFEQGNPISVLDGIFVTIKDDIDCLPHPTNGGTTWLHE 224

Query: 79  GSVVPRDAGVVTRLRKAGAIILGKASLSEWANFRSYGAPSGWNARAGHGREPYTLGDP-- 138
              V +D+ VV++LR  GAI+LGKA++ E          +G N+  G  R P+   DP  
Sbjct: 225 DRSVEKDSAVVSKLRSCGAILLGKANMHELGM-----GTTGNNSNYGTTRNPH---DPKR 284

Query: 139 --CGSSSGSAISVSANMAAVTLGTETDGSILCPCSQNSVVGIKPTVGLTSRAGVVPISPR 198
              GSSSGSA  V+A + +  LGT+  GS+  P +   + G+K T G T   G +     
Sbjct: 285 YTGGSSSGSAAIVAAGLCSAALGTDGGGSVRIPSALCGITGLKTTYGRTDMTGSLCEGGT 344

Query: 199 QDTVGPICRTVSDAAYVLDVIVGTDALDNETYKTS-----RYIPGGGYGQFLRANGLKGK 258
            + +GP+  ++ DA  V   I+G+ + D    K S     + +   G      +N +   
Sbjct: 345 VEIIGPLASSLEDAFLVYAAILGSSSADRYNLKPSPPCFPKLLSHNG------SNAIGSL 404

Query: 259 RLGIVEDLFS-VTEPFLIQAFEEIFTILRKRGAILVNNLKIDNLGIIYSAESGEDIALLN 318
           RLG     F+ V+   +    E+I  +L       V  + +  L  + +A     I++ +
Sbjct: 405 RLGKYTKWFNDVSSSDISDKCEDILKLLSNNHGCKVVEIVVPELEEMRAAHV---ISIGS 464

Query: 319 EFKVSLNAYL----RELISSPVRT---------LSDVIAFNQKHSKVEKIKEYRQDLFLQ 378
               SL  Y        +S   RT          SD IA         ++ EY  ++F  
Sbjct: 465 PTLSSLTPYCEAGKNSKLSYDTRTSFAIFRSFSASDYIA---AQCLRRRLMEYHLNIFKD 524

Query: 379 AE--KTKGIGMEAVLSKLSKLSKDGLEKTITENKLDAIVTPDSSISTLLA-IGGFPGITV 438
            +   T   GM A +     +  D L+   T  +    VT D     L A + GFP I+V
Sbjct: 525 VDVIVTPTTGMTAPV-----IPPDALKNGETNIQ----VTTDLMRFVLAANLLGFPAISV 584

Query: 439 PAGYLPKLGTPFGIHFGGLKGFEPKLIEIAYDFEQATKRRKPPPL 451
           P GY  K G P G+   G    E  ++ +A   E+     K P +
Sbjct: 585 PVGY-DKEGLPIGLQIMGRPWAEATVLGLAAAVEELAPVTKKPAI 599

BLAST of Lag0009282 vs. TAIR 10
Match: AT5G07360.2 (Amidase family protein )

HSP 1 Score: 80.5 bits (197), Expect = 3.8e-15
Identity = 60/173 (34.68%), Postives = 85/173 (49.13%), Query Frame = 0

Query: 44  LHGIPVLVKDNIATKDKLNTTAGSFALLGSVVPRDAGVVTRLRKAGAIILGKASLSEWAN 103
           LHGIP  +KD +A      TT GS +     +  +A V  RL+ +GA+++ K        
Sbjct: 268 LHGIPYGLKDIVAVPG-YKTTWGSTSFKDQFLDIEAWVYKRLKASGAVLVAKLVTG---- 327

Query: 104 FRSYGAPSGWNARAGHGREPYTLGD-PCGSSSGSAISVSANMAAVTLGTETDGSILCPCS 163
             S      W    G  R P+ + +   GSS+G A S SA       G+ET GS+  P +
Sbjct: 328 --SMAYDDIW--FGGRTRNPWNIEEFSTGSSAGPAASTSA-------GSETAGSMTYPAA 387

Query: 164 QNSVVGIKPTVGLTSRAGVVPISPRQDTVGPICRTVSDAAYVLDVIVGTDALD 216
           +  +  ++PT G   R GV+ IS   D +GP CRT +D A +LD I G D  D
Sbjct: 388 RCGITALRPTFGSVGRTGVMSISESLDKLGPFCRTAADCAVILDAIKGKDPDD 424

BLAST of Lag0009282 vs. TAIR 10
Match: AT1G08980.1 (amidase 1 )

HSP 1 Score: 65.5 bits (158), Expect = 1.3e-10
Identity = 46/125 (36.80%), Postives = 65/125 (52.00%), Query Frame = 0

Query: 73  SVVPRDAGVVTRLRKAGAIILGKASLSEWANFRSYGAPSGWNARAGHGREPYTLGD-PCG 132
           S     A VV+ L +AGA  LG   + E A      + +G NA  G  R P      P G
Sbjct: 58  SAATSTAPVVSSLLEAGATALGITIMDEMAY-----SINGENAHYGTPRNPIAFDRVPGG 117

Query: 133 SSSGSAISVSANMAAVTLGTETDGSILCPCSQNSVVGIKPTVGLTSRAGVVPISPRQDTV 192
           SSSGSA++V+A +   ++GT+T GS+  P S   + G +P+ G  S  G+ P++   DTV
Sbjct: 118 SSSGSAVAVAARLVDFSIGTDTGGSVRVPASYCGIFGFRPSHGAVSTVGLTPMAQSFDTV 177

Query: 193 GPICR 197
           G   R
Sbjct: 178 GWFAR 177