Lag0009267 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0009267
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionTriosephosphate isomerase
Locationchr9: 37531807 .. 37535595 (-)
RNA-Seq ExpressionLag0009267
SyntenyLag0009267
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGTGGTGTCTACATCTCTTGCATCTCAACTCTCAGCGGTCCGATTGTCCAGTTCCAACTCCGACATTTCTCACACTCTGTTTCAGAACGTCCAATCACAAATCCGCCTTGCATCCTCGCGGAAAGGATCCCGTGGCGTTGTCACCATGGCCGGCTCCGGCAAGGTATTGAACCTTTTTCGTTATTTGTTCTTGATCTGCGCTCGAATTGTTTTCCGCTACCTGAAGAATTTTAGGTTAGTGCGTCGTTTGCAGTAGGGAGAGAGGAGGAGAAGATGTAAGGAAAGTTGTTGCTTATTTTTCGTATTTGAAGGCGTTAGAGAATTCGGAGTAGGCGATGATTCCAAGAACAAATCTGTTTAAGCTATATACAGATTAATTTTGATTTGTCCATTTTTTTTATAGGTCACACTTGTGAACTGGGATTAGGACTCGGTCAGGATTTAATTATTGCTGATAAGTTTGGTGTTTCATATCTCTCAAGCCGTCTAGCGTTTTTGAATAACTATGGAGTATACTTATTGCCACATCTTTGATGATTTTTTCCAATTTGTTTTTTAAACTAATCCTTAAATTTAGAGGGCCATGGTTCTTACGGTATTTTGTTTATTTTAAATCGGTTTTCTATGATCTCTTGTTTGCACATATATATCTTTTCACTTCTAATCATTATCATCCTTTGATGTATTTGCAGTTCTTCGTTGGTGGAAACTGGAAATGCGTAAGTATCTTCATTATCAATAATCTTATAACTAAAGGTCTGTGATGTTTGTTTGTTTGTTTTTTTTTTTTTTTTTTTGAGGGAAAAGGTCTGTGATGTTTGTCTGTTTGTTTTTTTTTTTTTTGAGGGAAAAGGTCTGTGATGATTAGTTTTATATGTCAAGCAAAAAAAAAAAAAAAAGTGTGTATGTACAGTGTAAATGTATGCTGTGATTTCTCTCCGAGTCACTCTATGTTTCTATTCTGTCCATAATCTCACGAGTCTGAAACGCAAGTCTTATCACCATCTCTCTAGAACTTTTATTCTCTGTAAGAACTTGTGTCAAACATTGATGAGAATGTTGTTGATTTTCATCTATTTTTCCAGTATCATTTCTACTGTCTACTATTCATTGTTTTAGTTAGATTTGTATGAAACTGTTGTAAGCTACTAAATGGGAGTATGTACAAATCATTGGAAATAGAAGAAACCTTAGAAAAATAGACGACATTTCTTTTTTGTCTCTATATCTTCATTTTCATAGGCAAAACGGTTTAAGTTTTGAAACTGGTTAGGTTCAAGCTATCCCTTCTTACGAAGGTTCTCCTGCCACTTCTATATGAGATACCACTTTTGAATTATTTTGATTGAAGTCTTTAGAAACTTTCCTATCTTCTAATACTTAGTCATTAGCTTTGTTTTTTTTACTACTCTTAAGTTTTTAAAGTGTACTGGATCCATTGTGGTATCCAAAATGAAGACATCTATTTGCCTGCTAGGGAGGCTTGACTATGATCCAAGATGATTGAACTACTAATATCCCCCCAAAGTACTTCTGATTTGCAATTGACATATTTTGATTCTTCCCTGTTTATTTTAATTTCCAGAATGGTACAAAGGAATCTATAACCAAGCTTGTCTCAGACTTGAACAATGTAAAGTTGGAGGATGATGTTGGTAAGTAACGTTTGATATTGGATGCCTGTTATCAATTGCAATCAATGTATGCAATTCTTGCTAAAAAGAGGTTGAACTTGATACCAGCTTGTTTCTCACATTTTTGTTCTAAATTCCAATAGTCCTTCTCTTCATACTGAAAGCTGTTTTTTTTTTTTCATAATTCTTTTTTCTCTTCACATTTGTTATTCTACTCTTCAAGTACATTCTTCTGAGAAAAGAAAATTTGATATGTGTGGAGAAATGCAGATGTTGTTGTAGCACCTCCATTTGTCTACATTGGTCAAGTGAAGAGTTCATTGGCATCGAGGATTGAGATTTCTGCCCAAAACTCATGGGTGAGCAAGGGTGGTGCTTTTACCGGAGAAATCAGGTTGGCTGGCTAGCCCTTGATGAAACATTTTAATTATTGAGGGCCTCTAAAAATTTATTTATCTTAATTCAACCGTTATTTCTGTTGATTATTCTTTGGACATAAGTTCTCGAAAAATTATTTTTAGTGCATGCCAACTCTTGATTTTACACCATTTGAGGAGGATGAAAACTTTTGTCTGCTTGTTTCATGATCTGCAAATGAGATAATGATAATGTAAAACACAAGTATACTGGCGTTCAATTTGATGATACTTAGAAGCTTGAACTGCTTTAAGTTGTAGTGTTTTGTAAATTTTACTTTTCAAAATTTAAGAGTTTGAGAAATATGACTCCCTAAGAGATTTTGATGTTTGTAAACAACTCTTGCTTAAATGTATATTCTTTCAATTTTATCCTAATGAAAGCGTGGTTTCTTATTTAAAAAAAAAAAAACACTCATAACTTTTTAATTGAAGTAGTTAATAATCCTTTTTTGAACCTTTTCACTTTTTAAAGTGAAGTTTTTTGAAAAGCAATTTTATGATTGAGCTAACTTTTATTTAACATCAGATGCCTACTAAACTTGTACTCGTTTTTTTTTTTCCTTTTGCATAAATGTCATGTGTTTTGTGTTTTTCCACTTACAAATGTATATCAGATTTTATCATTGTTAAAATTAATTTGTATGTGCTGCAGTGTTGAACAATTGAAAGATATAGGCTGCAAGTGGGTTATCCTTGGGCACTCTGAACGGAGACATATCATTGGTGAAGATGACCAGGTGAATGTTTTGAAATTGAGCTTTGAGTGGTTTCCAAGAAATAAGTGTTCAGGCTGATTCTCTCCAACGCTGATGATGCTTTGGCTTTGGCAACTATAGTTTATAGGAAAGAAGGCTGCCTATGCCTTGAGTGAGGGTCTTGGAGTAATAGCTTGCATTGGGGAATTACTAGAGGAAAGGGAAGCTGGGAAAACTTTTGATGTTTGTTTCCAACAATTGAAAGCTTTTGCAGGCAAGTTGCTGGCTCATTCTTTATTTCATTTTCACGATCATATTATTGTCATTGTCACTGATCTGAGAACAAAAAAGTGCTGGAGTTTTCTGTAACTTAGTGATTTAGATTCCCATTGACTGCAGATGCGGTTCCTAGTTGGGATGCTGTTGTTATTGCATACGAGCCTGTATGGGCCATTGGAACTGGCAAAGTGGCTACCCCTGAGCAAGCACAGGAGGTGCATGCAGCAGTTCGTGATTGGCTTAAGAAGAACGTCTCACCAGAAGTTGCATCTAAAACACGAATTATTTATGGAGGTAACTGCCACTTTCATTTGTATGCTGGAATGTGCTACTCACAACTTTTGGCCGATTTAGAAATAAATAAATTAACTTGTGCAAACTTGTTATTCTGAAGTCCATGTTTCTTATCACTGCCATTTTTTTTATAAGAAACTTGTAACATTGCCATTGTGAATAACAATCTAGAGGTGCTCACGTGTAAGGAACGATATTATTGTCAGGATCTGTAAATGGAAGTAATTGTGCTGAGCTTGCAAAAAAAGAGGATATAGATGGATTTCTAGTTGGAGGTGCTTCGTTGAAGGTGCATTTCTCCTCTCCCCTTACGTTTTTCTAATGTTTACTATCTAACAATAAAAGAAAATTAGAGAATCACACTCAAAGTTCCGATCTCAACTTCTGTTCCATGTTGCAATTGCAGGGGCCTGAATTTGGAACAATCGTTAATTCTGTAACTGCCAAGAAAGTCGCTGCTTGA

mRNA sequence

ATGGCGGTGGTGTCTACATCTCTTGCATCTCAACTCTCAGCGGTCCGATTGTCCAGTTCCAACTCCGACATTTCTCACACTCTGTTTCAGAACGTCCAATCACAAATCCGCCTTGCATCCTCGCGGAAAGGATCCCGTGGCGTTGTCACCATGGCCGGCTCCGGCAAGTTCTTCGTTGGTGGAAACTGGAAATGCAATGGTACAAAGGAATCTATAACCAAGCTTGTCTCAGACTTGAACAATGTAAAGTTGGAGGATGATGTTGATGTTGTTGTAGCACCTCCATTTGTCTACATTGGTCAAGTGAAGAGTTCATTGGCATCGAGGATTGAGATTTCTGCCCAAAACTCATGGGTGAGCAAGGGTGGTGCTTTTACCGGAGAAATCAGTGTTGAACAATTGAAAGATATAGGCTGCAAGTGGGTTATCCTTGGGCACTCTGAACGGAGACATATCATTGGTGAAGATGACCAGTTTATAGGAAAGAAGGCTGCCTATGCCTTGAGTGAGGGTCTTGGAGTAATAGCTTGCATTGGGGAATTACTAGAGGAAAGGGAAGCTGGGAAAACTTTTGATGTTTGTTTCCAACAATTGAAAGCTTTTGCAGATGCGGTTCCTAGTTGGGATGCTGTTGTTATTGCATACGAGCCTGTATGGGCCATTGGAACTGGCAAAGTGGCTACCCCTGAGCAAGCACAGGAGGTGCATGCAGCAGTTCGTGATTGGCTTAAGAAGAACGTCTCACCAGAAGTTGCATCTAAAACACGAATTATTTATGGAGGATCTGTAAATGGAAGTAATTGTGCTGAGCTTGCAAAAAAAGAGGATATAGATGGATTTCTAGTTGGAGGTGCTTCGTTGAAGGGGCCTGAATTTGGAACAATCGTTAATTCTGTAACTGCCAAGAAAGTCGCTGCTTGA

Coding sequence (CDS)

ATGGCGGTGGTGTCTACATCTCTTGCATCTCAACTCTCAGCGGTCCGATTGTCCAGTTCCAACTCCGACATTTCTCACACTCTGTTTCAGAACGTCCAATCACAAATCCGCCTTGCATCCTCGCGGAAAGGATCCCGTGGCGTTGTCACCATGGCCGGCTCCGGCAAGTTCTTCGTTGGTGGAAACTGGAAATGCAATGGTACAAAGGAATCTATAACCAAGCTTGTCTCAGACTTGAACAATGTAAAGTTGGAGGATGATGTTGATGTTGTTGTAGCACCTCCATTTGTCTACATTGGTCAAGTGAAGAGTTCATTGGCATCGAGGATTGAGATTTCTGCCCAAAACTCATGGGTGAGCAAGGGTGGTGCTTTTACCGGAGAAATCAGTGTTGAACAATTGAAAGATATAGGCTGCAAGTGGGTTATCCTTGGGCACTCTGAACGGAGACATATCATTGGTGAAGATGACCAGTTTATAGGAAAGAAGGCTGCCTATGCCTTGAGTGAGGGTCTTGGAGTAATAGCTTGCATTGGGGAATTACTAGAGGAAAGGGAAGCTGGGAAAACTTTTGATGTTTGTTTCCAACAATTGAAAGCTTTTGCAGATGCGGTTCCTAGTTGGGATGCTGTTGTTATTGCATACGAGCCTGTATGGGCCATTGGAACTGGCAAAGTGGCTACCCCTGAGCAAGCACAGGAGGTGCATGCAGCAGTTCGTGATTGGCTTAAGAAGAACGTCTCACCAGAAGTTGCATCTAAAACACGAATTATTTATGGAGGATCTGTAAATGGAAGTAATTGTGCTGAGCTTGCAAAAAAAGAGGATATAGATGGATTTCTAGTTGGAGGTGCTTCGTTGAAGGGGCCTGAATTTGGAACAATCGTTAATTCTGTAACTGCCAAGAAAGTCGCTGCTTGA

Protein sequence

MAVVSTSLASQLSAVRLSSSNSDISHTLFQNVQSQIRLASSRKGSRGVVTMAGSGKFFVGGNWKCNGTKESITKLVSDLNNVKLEDDVDVVVAPPFVYIGQVKSSLASRIEISAQNSWVSKGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVIACIGELLEEREAGKTFDVCFQQLKAFADAVPSWDAVVIAYEPVWAIGTGKVATPEQAQEVHAAVRDWLKKNVSPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVTAKKVAA
Homology
BLAST of Lag0009267 vs. NCBI nr
Match: XP_022987010.1 (triosephosphate isomerase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 574.3 bits (1479), Expect = 6.2e-160
Identity = 292/306 (95.42%), Postives = 299/306 (97.71%), Query Frame = 0

Query: 1   MAVVSTSLASQLSAVRLSSSNSDISHTLFQNVQSQIRLASSRKGSRGVVTMAGSGKFFVG 60
           MAVVSTSLASQLSA+RLS+S SDIS TLFQNV SQIRLASSRKGSRGVVTMAGSGKFFVG
Sbjct: 1   MAVVSTSLASQLSAIRLSTSKSDISQTLFQNVHSQIRLASSRKGSRGVVTMAGSGKFFVG 60

Query: 61  GNWKCNGTKESITKLVSDLNNVKLEDDVDVVVAPPFVYIGQVKSSLASRIEISAQNSWVS 120
           GNWKCNGTKESITKLVSDLNNVKLEDDVDVVVAPPF+YI QVKSSLA RIEISAQNSWVS
Sbjct: 61  GNWKCNGTKESITKLVSDLNNVKLEDDVDVVVAPPFLYIDQVKSSLAPRIEISAQNSWVS 120

Query: 121 KGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVIACIGE 180
           KGGAFTGEISVEQLKDIGCKWVILGHSERRH+IGEDDQFIGKKAAYALSEGLGVIACIGE
Sbjct: 121 KGGAFTGEISVEQLKDIGCKWVILGHSERRHVIGEDDQFIGKKAAYALSEGLGVIACIGE 180

Query: 181 LLEEREAGKTFDVCFQQLKAFADAVPSWDAVVIAYEPVWAIGTGKVATPEQAQEVHAAVR 240
           LLEEREAGKTFDVCFQQLKA+ADAVPSWD +VIAYEPVWAIGTGKVATP+QAQEVHAAVR
Sbjct: 181 LLEEREAGKTFDVCFQQLKAYADAVPSWDGIVIAYEPVWAIGTGKVATPDQAQEVHAAVR 240

Query: 241 DWLKKNVSPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVT 300
           DWLKKNVSPEVASKTRIIYGGSVNG NCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVT
Sbjct: 241 DWLKKNVSPEVASKTRIIYGGSVNGGNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVT 300

Query: 301 AKKVAA 307
           AKKVAA
Sbjct: 301 AKKVAA 306

BLAST of Lag0009267 vs. NCBI nr
Match: XP_022146193.1 (triosephosphate isomerase, chloroplastic [Momordica charantia])

HSP 1 Score: 573.9 bits (1478), Expect = 8.1e-160
Identity = 294/306 (96.08%), Postives = 296/306 (96.73%), Query Frame = 0

Query: 1   MAVVSTSLASQLSAVRLSSSNSDISHTLFQNVQSQIRLASSRKGSRGVVTMAGSGKFFVG 60
           MAVVSTSLASQLSAVRLSSS SDIS TLFQNV SQIRLASSRKGSRGV TMAGSGKFFVG
Sbjct: 1   MAVVSTSLASQLSAVRLSSSKSDISQTLFQNVHSQIRLASSRKGSRGVFTMAGSGKFFVG 60

Query: 61  GNWKCNGTKESITKLVSDLNNVKLEDDVDVVVAPPFVYIGQVKSSLASRIEISAQNSWVS 120
           GNWKCNGTKESI KLVSDLNN KLEDDVDVVVAPPFVYI QVKSSLASRIEISAQNSWVS
Sbjct: 61  GNWKCNGTKESIAKLVSDLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVS 120

Query: 121 KGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVIACIGE 180
           KGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVIACIGE
Sbjct: 121 KGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVIACIGE 180

Query: 181 LLEEREAGKTFDVCFQQLKAFADAVPSWDAVVIAYEPVWAIGTGKVATPEQAQEVHAAVR 240
           LLEEREAGKTFDVCFQQLKAFADA+PSWD VVIAYEPVWAIGTGKVATPEQAQEVHAAVR
Sbjct: 181 LLEEREAGKTFDVCFQQLKAFADAIPSWDGVVIAYEPVWAIGTGKVATPEQAQEVHAAVR 240

Query: 241 DWLKKNVSPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVT 300
           DWLKKN+SPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSV 
Sbjct: 241 DWLKKNISPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVA 300

Query: 301 AKKVAA 307
           AKKV A
Sbjct: 301 AKKVVA 306

BLAST of Lag0009267 vs. NCBI nr
Match: KAG6605006.1 (Triosephosphate isomerase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 573.9 bits (1478), Expect = 8.1e-160
Identity = 293/306 (95.75%), Postives = 300/306 (98.04%), Query Frame = 0

Query: 1   MAVVSTSLASQLSAVRLSSSNSDISHTLFQNVQSQIRLASSRKGSRGVVTMAGSGKFFVG 60
           MAVVSTSLASQLSAVRLS SNSDIS TL QNV+SQIRLASSRKGSRGVVTMAGSGKFFVG
Sbjct: 1   MAVVSTSLASQLSAVRLSGSNSDISQTLLQNVRSQIRLASSRKGSRGVVTMAGSGKFFVG 60

Query: 61  GNWKCNGTKESITKLVSDLNNVKLEDDVDVVVAPPFVYIGQVKSSLASRIEISAQNSWVS 120
           GNWKCNGTKESI+KLV+DLNN KLEDDVDVVVAPPFVYI QVKSSLASRIEISAQNSWVS
Sbjct: 61  GNWKCNGTKESISKLVADLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVS 120

Query: 121 KGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVIACIGE 180
           KGGAFTGEISVEQLKD+GCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVIACIGE
Sbjct: 121 KGGAFTGEISVEQLKDLGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVIACIGE 180

Query: 181 LLEEREAGKTFDVCFQQLKAFADAVPSWDAVVIAYEPVWAIGTGKVATPEQAQEVHAAVR 240
           LLEEREAGKTFDVCF+QLKAFADAVPSWD +VIAYEPVWAIGTGKVA+PEQAQEVHAAVR
Sbjct: 181 LLEEREAGKTFDVCFRQLKAFADAVPSWDGIVIAYEPVWAIGTGKVASPEQAQEVHAAVR 240

Query: 241 DWLKKNVSPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVT 300
           DWLKKNVSPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVT
Sbjct: 241 DWLKKNVSPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVT 300

Query: 301 AKKVAA 307
           AKKVAA
Sbjct: 301 AKKVAA 306

BLAST of Lag0009267 vs. NCBI nr
Match: XP_023007543.1 (triosephosphate isomerase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 573.2 bits (1476), Expect = 1.4e-159
Identity = 292/306 (95.42%), Postives = 300/306 (98.04%), Query Frame = 0

Query: 1   MAVVSTSLASQLSAVRLSSSNSDISHTLFQNVQSQIRLASSRKGSRGVVTMAGSGKFFVG 60
           MAVVSTSLASQLSAVRLS SNSDIS TL QNV+SQIRLASSRKGSRGVVTMAGSGKFFVG
Sbjct: 1   MAVVSTSLASQLSAVRLSGSNSDISQTLLQNVRSQIRLASSRKGSRGVVTMAGSGKFFVG 60

Query: 61  GNWKCNGTKESITKLVSDLNNVKLEDDVDVVVAPPFVYIGQVKSSLASRIEISAQNSWVS 120
           GNWKCNGTKESITKLV+DLNN KLEDDVD+VVAPPFVYI QVKSSLASRIEISAQNSWVS
Sbjct: 61  GNWKCNGTKESITKLVADLNNAKLEDDVDIVVAPPFVYIDQVKSSLASRIEISAQNSWVS 120

Query: 121 KGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVIACIGE 180
           KGGAFTGEISVEQLKD+GCKWVILGHSERRHIIGEDDQFIGKKA+YALSEGLGVIACIGE
Sbjct: 121 KGGAFTGEISVEQLKDLGCKWVILGHSERRHIIGEDDQFIGKKASYALSEGLGVIACIGE 180

Query: 181 LLEEREAGKTFDVCFQQLKAFADAVPSWDAVVIAYEPVWAIGTGKVATPEQAQEVHAAVR 240
           LLEEREAGKTFDVCF+QLKAFADAVPSWD +VIAYEPVWAIGTGKVA+PEQAQEVHAAVR
Sbjct: 181 LLEEREAGKTFDVCFRQLKAFADAVPSWDDIVIAYEPVWAIGTGKVASPEQAQEVHAAVR 240

Query: 241 DWLKKNVSPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVT 300
           DWLKKNVSPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVT
Sbjct: 241 DWLKKNVSPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVT 300

Query: 301 AKKVAA 307
           AKKVAA
Sbjct: 301 AKKVAA 306

BLAST of Lag0009267 vs. NCBI nr
Match: XP_022947479.1 (triosephosphate isomerase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 572.8 bits (1475), Expect = 1.8e-159
Identity = 292/306 (95.42%), Postives = 300/306 (98.04%), Query Frame = 0

Query: 1   MAVVSTSLASQLSAVRLSSSNSDISHTLFQNVQSQIRLASSRKGSRGVVTMAGSGKFFVG 60
           MAVVSTSLASQLSAVRLS SNSDIS TL QNV+SQIRLASSRKGSRGVVTMAGSGKFFVG
Sbjct: 1   MAVVSTSLASQLSAVRLSGSNSDISQTLLQNVRSQIRLASSRKGSRGVVTMAGSGKFFVG 60

Query: 61  GNWKCNGTKESITKLVSDLNNVKLEDDVDVVVAPPFVYIGQVKSSLASRIEISAQNSWVS 120
           GNWKCNGTKESI+KLV+DLNN KLEDDVDVVVAPPFVYI QVKSSLASRIEISAQNSWVS
Sbjct: 61  GNWKCNGTKESISKLVADLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVS 120

Query: 121 KGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVIACIGE 180
           KGGAFTGEISVEQLKD+GCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGV+ACIGE
Sbjct: 121 KGGAFTGEISVEQLKDLGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVMACIGE 180

Query: 181 LLEEREAGKTFDVCFQQLKAFADAVPSWDAVVIAYEPVWAIGTGKVATPEQAQEVHAAVR 240
           LLEEREAGKTFDVCF+QLKAFADAVPSWD +VIAYEPVWAIGTGKVA+PEQAQEVHAAVR
Sbjct: 181 LLEEREAGKTFDVCFRQLKAFADAVPSWDGIVIAYEPVWAIGTGKVASPEQAQEVHAAVR 240

Query: 241 DWLKKNVSPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVT 300
           DWLKKNVSPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVT
Sbjct: 241 DWLKKNVSPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVT 300

Query: 301 AKKVAA 307
           AKKVAA
Sbjct: 301 AKKVAA 306

BLAST of Lag0009267 vs. ExPASy Swiss-Prot
Match: P48496 (Triosephosphate isomerase, chloroplastic OS=Spinacia oleracea OX=3562 GN=TPIP1 PE=1 SV=1)

HSP 1 Score: 490.0 bits (1260), Expect = 2.0e-137
Identity = 255/323 (78.95%), Postives = 280/323 (86.69%), Query Frame = 0

Query: 1   MAVVSTSLASQL----------------SAVRLSSSNSDISHTLFQNVQSQIRL-ASSRK 60
           MAVVSTSLASQ+                S ++L +S S  S + FQNV S +RL +SSR+
Sbjct: 1   MAVVSTSLASQITNPNSAVSTQFSGLRRSFLKLENSVSTQS-SFFQNVDSHLRLSSSSRR 60

Query: 61  GSRGVVTMAGSGKFFVGGNWKCNGTKESITKLVSDLNNVKLEDDVDVVVAPPFVYIGQVK 120
             RGVV MAGSGKFFVGGNWKCNGTKESITKLVSDLN+  LE DVDVVVAPPFVYI QVK
Sbjct: 61  CPRGVVAMAGSGKFFVGGNWKCNGTKESITKLVSDLNSATLEADVDVVVAPPFVYIDQVK 120

Query: 121 SSLASRIEISAQNSWVSKGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKK 180
           SSL  R+EISAQN W+ KGGAFTGEISVEQLKD+GC+WVILGHSERRH+IGE ++FIGKK
Sbjct: 121 SSLTGRVEISAQNCWIGKGGAFTGEISVEQLKDLGCQWVILGHSERRHVIGEQNEFIGKK 180

Query: 181 AAYALSEGLGVIACIGELLEEREAGKTFDVCFQQLKAFADAVPSWDAVVIAYEPVWAIGT 240
           AAYAL++G+GVIACIGELLEEREAGKTFDVC+QQLKAFADA+PSWD VV+AYEPVWAIGT
Sbjct: 181 AAYALNQGVGVIACIGELLEEREAGKTFDVCYQQLKAFADALPSWDNVVVAYEPVWAIGT 240

Query: 241 GKVATPEQAQEVHAAVRDWLKKNVSPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVG 300
           GKVA+P+QAQEVH AVRDWLKKNVS EVASKTRIIYGGSVNG NCAELAK+EDIDGFLVG
Sbjct: 241 GKVASPDQAQEVHVAVRDWLKKNVSEEVASKTRIIYGGSVNGGNCAELAKQEDIDGFLVG 300

Query: 301 GASLKGPEFGTIVNSVTAKKVAA 307
           GASLKGPEF TIVNSVTAKKVAA
Sbjct: 301 GASLKGPEFATIVNSVTAKKVAA 322

BLAST of Lag0009267 vs. ExPASy Swiss-Prot
Match: Q9M4S8 (Triosephosphate isomerase, chloroplastic OS=Fragaria ananassa OX=3747 GN=TPI PE=2 SV=1)

HSP 1 Score: 476.9 bits (1226), Expect = 1.8e-133
Identity = 249/317 (78.55%), Postives = 274/317 (86.44%), Query Frame = 0

Query: 1   MAVVSTSLASQL----------SAVRLSSSNSDISH-TLFQNVQSQIRLASSRKGSRGVV 60
           MAV STSLASQL          S +R S    D SH +LFQ++      +SSRK SR VV
Sbjct: 1   MAVASTSLASQLSGPKSLSQPYSGLRRSCPKLDQSHSSLFQHLSLS---SSSRKASRAVV 60

Query: 61  TMAGSGKFFVGGNWKCNGTKESITKLVSDLNNVKLEDDVDVVVAPPFVYIGQVKSSLASR 120
            MAG+GKFFVGGNWKCNGTK+ I+KLVSDLN+ KLE DVDVVVAPPF+Y+ QVKSSL  R
Sbjct: 61  AMAGTGKFFVGGNWKCNGTKDLISKLVSDLNSAKLEPDVDVVVAPPFLYLDQVKSSLTDR 120

Query: 121 IEISAQNSWVSKGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALS 180
           IEIS QNSWV+KGGAFTGEISVEQLKDIG KWVILGHSERRH+IGEDDQFIGKKAAYAL+
Sbjct: 121 IEISGQNSWVAKGGAFTGEISVEQLKDIGRKWVILGHSERRHVIGEDDQFIGKKAAYALN 180

Query: 181 EGLGVIACIGELLEEREAGKTFDVCFQQLKAFADAVPSWDAVVIAYEPVWAIGTGKVATP 240
           EGLGVIACIGE LEEREAGKTFDVC++QLKAFADAVPSW+ +V+AYEPVWAIGTGKVA+P
Sbjct: 181 EGLGVIACIGEKLEEREAGKTFDVCYEQLKAFADAVPSWENIVVAYEPVWAIGTGKVASP 240

Query: 241 EQAQEVHAAVRDWLKKNVSPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKG 300
           +QAQEVH AVR+WLKKNVS EVASKTRIIYGGSVNG N AELAK+EDIDGFLVGGASLKG
Sbjct: 241 QQAQEVHVAVREWLKKNVSAEVASKTRIIYGGSVNGGNSAELAKEEDIDGFLVGGASLKG 300

Query: 301 PEFGTIVNSVTAKKVAA 307
           PEF TIVN+VT+KKVAA
Sbjct: 301 PEFATIVNAVTSKKVAA 314

BLAST of Lag0009267 vs. ExPASy Swiss-Prot
Match: Q9SKP6 (Triosephosphate isomerase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIM PE=1 SV=1)

HSP 1 Score: 467.6 bits (1202), Expect = 1.1e-130
Identity = 242/315 (76.83%), Postives = 260/315 (82.54%), Query Frame = 0

Query: 1   MAVVSTSLASQLSAVRLSSSNSDIS-----HTLFQNVQSQIRLASSRKGS----RGVVTM 60
           MA  S +     S +R  S   D +      + F  V S  RL SS   S    RGVV M
Sbjct: 1   MAATSLTAPPSFSGLRRISPKLDAAAVSSHQSFFHRVNSSTRLVSSSSSSHRSPRGVVAM 60

Query: 61  AGSGKFFVGGNWKCNGTKESITKLVSDLNNVKLEDDVDVVVAPPFVYIGQVKSSLASRIE 120
           AGSGKFFVGGNWKCNGTK+SI KL+SDLN+  LE DVDVVV+PPFVYI QVKSSL  RI+
Sbjct: 61  AGSGKFFVGGNWKCNGTKDSIAKLISDLNSATLEADVDVVVSPPFVYIDQVKSSLTDRID 120

Query: 121 ISAQNSWVSKGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEG 180
           IS QNSWV KGGAFTGEISVEQLKD+GCKWVILGHSERRH+IGE D+FIGKKAAYALSEG
Sbjct: 121 ISGQNSWVGKGGAFTGEISVEQLKDLGCKWVILGHSERRHVIGEKDEFIGKKAAYALSEG 180

Query: 181 LGVIACIGELLEEREAGKTFDVCFQQLKAFADAVPSWDAVVIAYEPVWAIGTGKVATPEQ 240
           LGVIACIGE LEEREAGKTFDVCF QLKAFADAVPSWD +V+AYEPVWAIGTGKVA+P+Q
Sbjct: 181 LGVIACIGEKLEEREAGKTFDVCFAQLKAFADAVPSWDNIVVAYEPVWAIGTGKVASPQQ 240

Query: 241 AQEVHAAVRDWLKKNVSPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPE 300
           AQEVH AVR WLKKNVS EVASKTRIIYGGSVNG N AELAK+EDIDGFLVGGASLKGPE
Sbjct: 241 AQEVHVAVRGWLKKNVSEEVASKTRIIYGGSVNGGNSAELAKEEDIDGFLVGGASLKGPE 300

Query: 301 FGTIVNSVTAKKVAA 307
           F TIVNSVT+KKVAA
Sbjct: 301 FATIVNSVTSKKVAA 315

BLAST of Lag0009267 vs. ExPASy Swiss-Prot
Match: P46225 (Triosephosphate isomerase, chloroplastic OS=Secale cereale OX=4550 PE=1 SV=1)

HSP 1 Score: 440.7 bits (1132), Expect = 1.4e-122
Identity = 214/259 (82.63%), Postives = 233/259 (89.96%), Query Frame = 0

Query: 48  VVTMAGSGKFFVGGNWKCNGTKESITKLVSDLNNVKLEDDVDVVVAPPFVYIGQVKSSLA 107
           +V MAGSGKFFVGGNWKCNGTKESI+KLVSDLN   LE DVDVVVAPPF+YI QVKSSL 
Sbjct: 40  LVAMAGSGKFFVGGNWKCNGTKESISKLVSDLNAATLESDVDVVVAPPFIYIDQVKSSLT 99

Query: 108 SRIEISAQNSWVSKGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYA 167
            RIE+SAQN+W+ KGGAFTGEIS EQL DIGC+WVILGHSERRH+IGEDD+FIGKKAAYA
Sbjct: 100 DRIEVSAQNTWIGKGGAFTGEISAEQLVDIGCQWVILGHSERRHVIGEDDEFIGKKAAYA 159

Query: 168 LSEGLGVIACIGELLEEREAGKTFDVCFQQLKAFADAVPSWDAVVIAYEPVWAIGTGKVA 227
           LS+ L V+ACIGELLEEREAGKTFDVCF+Q+KAFAD +  W  VVIAYEPVWAIGTGKVA
Sbjct: 160 LSQNLKVMACIGELLEEREAGKTFDVCFKQMKAFADNITDWTNVVIAYEPVWAIGTGKVA 219

Query: 228 TPEQAQEVHAAVRDWLKKNVSPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASL 287
           +PEQAQEVHAAVRDWLK NVS +VAS  RIIYGGSVN +NCAELAKKEDIDGFLVGGASL
Sbjct: 220 SPEQAQEVHAAVRDWLKTNVSADVASTVRIIYGGSVNAANCAELAKKEDIDGFLVGGASL 279

Query: 288 KGPEFGTIVNSVTAKKVAA 307
           KGP+F TI NSVT+KKV A
Sbjct: 280 KGPDFATICNSVTSKKVTA 298

BLAST of Lag0009267 vs. ExPASy Swiss-Prot
Match: P48497 (Triosephosphate isomerase, cytosolic OS=Stellaria longipes OX=19744 GN=TPI PE=2 SV=1)

HSP 1 Score: 369.8 bits (948), Expect = 3.0e-101
Identity = 181/254 (71.26%), Postives = 210/254 (82.68%), Query Frame = 0

Query: 56  KFFVGGNWKCNGTKESITKLVSDLN--NVKLEDDVDVVVAPPFVYIGQVKSSLASRIEIS 115
           KFFVGGNWKCNGT+ES++K+V  LN   +   D V VVV+PP+V++    + L   I+++
Sbjct: 4   KFFVGGNWKCNGTQESVSKIVDTLNEPTIAANDVVTVVVSPPYVFLPDENAELKHEIQVA 63

Query: 116 AQNSWVSKGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLG 175
           AQN WV KGGAFTGE+S + L ++G  WVILGHSERR ++GE ++F+GKKAAYA SEGLG
Sbjct: 64  AQNCWVKKGGAFTGEVSAQMLANLGITWVILGHSERRTLLGESNEFVGKKAAYAQSEGLG 123

Query: 176 VIACIGELLEEREAGKTFDVCFQQLKAFAD-AVPSWDAVVIAYEPVWAIGTGKVATPEQA 235
           VIACIGELLEEREAGKTFDVCF+QLK+FA      WD VV+AYEPVWAIGTGKVA+PEQA
Sbjct: 124 VIACIGELLEEREAGKTFDVCFKQLKSFARFPAKPWDNVVVAYEPVWAIGTGKVASPEQA 183

Query: 236 QEVHAAVRDWLKKNVSPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEF 295
           QEVH AVRDWLK NVS EVASKTRIIYGGSVNG N   LA +ED+DGFLVGGASLKGPEF
Sbjct: 184 QEVHVAVRDWLKTNVSEEVASKTRIIYGGSVNGGNSLALAAQEDVDGFLVGGASLKGPEF 243

Query: 296 GTIVNSVTAKKVAA 307
            TI+NSVTAKKVAA
Sbjct: 244 ATIINSVTAKKVAA 257

BLAST of Lag0009267 vs. ExPASy TrEMBL
Match: A0A6J1JFM2 (triosephosphate isomerase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111484585 PE=3 SV=1)

HSP 1 Score: 574.3 bits (1479), Expect = 3.0e-160
Identity = 292/306 (95.42%), Postives = 299/306 (97.71%), Query Frame = 0

Query: 1   MAVVSTSLASQLSAVRLSSSNSDISHTLFQNVQSQIRLASSRKGSRGVVTMAGSGKFFVG 60
           MAVVSTSLASQLSA+RLS+S SDIS TLFQNV SQIRLASSRKGSRGVVTMAGSGKFFVG
Sbjct: 1   MAVVSTSLASQLSAIRLSTSKSDISQTLFQNVHSQIRLASSRKGSRGVVTMAGSGKFFVG 60

Query: 61  GNWKCNGTKESITKLVSDLNNVKLEDDVDVVVAPPFVYIGQVKSSLASRIEISAQNSWVS 120
           GNWKCNGTKESITKLVSDLNNVKLEDDVDVVVAPPF+YI QVKSSLA RIEISAQNSWVS
Sbjct: 61  GNWKCNGTKESITKLVSDLNNVKLEDDVDVVVAPPFLYIDQVKSSLAPRIEISAQNSWVS 120

Query: 121 KGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVIACIGE 180
           KGGAFTGEISVEQLKDIGCKWVILGHSERRH+IGEDDQFIGKKAAYALSEGLGVIACIGE
Sbjct: 121 KGGAFTGEISVEQLKDIGCKWVILGHSERRHVIGEDDQFIGKKAAYALSEGLGVIACIGE 180

Query: 181 LLEEREAGKTFDVCFQQLKAFADAVPSWDAVVIAYEPVWAIGTGKVATPEQAQEVHAAVR 240
           LLEEREAGKTFDVCFQQLKA+ADAVPSWD +VIAYEPVWAIGTGKVATP+QAQEVHAAVR
Sbjct: 181 LLEEREAGKTFDVCFQQLKAYADAVPSWDGIVIAYEPVWAIGTGKVATPDQAQEVHAAVR 240

Query: 241 DWLKKNVSPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVT 300
           DWLKKNVSPEVASKTRIIYGGSVNG NCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVT
Sbjct: 241 DWLKKNVSPEVASKTRIIYGGSVNGGNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVT 300

Query: 301 AKKVAA 307
           AKKVAA
Sbjct: 301 AKKVAA 306

BLAST of Lag0009267 vs. ExPASy TrEMBL
Match: A0A6J1CXY1 (triosephosphate isomerase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111015469 PE=3 SV=1)

HSP 1 Score: 573.9 bits (1478), Expect = 3.9e-160
Identity = 294/306 (96.08%), Postives = 296/306 (96.73%), Query Frame = 0

Query: 1   MAVVSTSLASQLSAVRLSSSNSDISHTLFQNVQSQIRLASSRKGSRGVVTMAGSGKFFVG 60
           MAVVSTSLASQLSAVRLSSS SDIS TLFQNV SQIRLASSRKGSRGV TMAGSGKFFVG
Sbjct: 1   MAVVSTSLASQLSAVRLSSSKSDISQTLFQNVHSQIRLASSRKGSRGVFTMAGSGKFFVG 60

Query: 61  GNWKCNGTKESITKLVSDLNNVKLEDDVDVVVAPPFVYIGQVKSSLASRIEISAQNSWVS 120
           GNWKCNGTKESI KLVSDLNN KLEDDVDVVVAPPFVYI QVKSSLASRIEISAQNSWVS
Sbjct: 61  GNWKCNGTKESIAKLVSDLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVS 120

Query: 121 KGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVIACIGE 180
           KGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVIACIGE
Sbjct: 121 KGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVIACIGE 180

Query: 181 LLEEREAGKTFDVCFQQLKAFADAVPSWDAVVIAYEPVWAIGTGKVATPEQAQEVHAAVR 240
           LLEEREAGKTFDVCFQQLKAFADA+PSWD VVIAYEPVWAIGTGKVATPEQAQEVHAAVR
Sbjct: 181 LLEEREAGKTFDVCFQQLKAFADAIPSWDGVVIAYEPVWAIGTGKVATPEQAQEVHAAVR 240

Query: 241 DWLKKNVSPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVT 300
           DWLKKN+SPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSV 
Sbjct: 241 DWLKKNISPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVA 300

Query: 301 AKKVAA 307
           AKKV A
Sbjct: 301 AKKVVA 306

BLAST of Lag0009267 vs. ExPASy TrEMBL
Match: A0A6J1L7Y2 (triosephosphate isomerase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111500005 PE=3 SV=1)

HSP 1 Score: 573.2 bits (1476), Expect = 6.7e-160
Identity = 292/306 (95.42%), Postives = 300/306 (98.04%), Query Frame = 0

Query: 1   MAVVSTSLASQLSAVRLSSSNSDISHTLFQNVQSQIRLASSRKGSRGVVTMAGSGKFFVG 60
           MAVVSTSLASQLSAVRLS SNSDIS TL QNV+SQIRLASSRKGSRGVVTMAGSGKFFVG
Sbjct: 1   MAVVSTSLASQLSAVRLSGSNSDISQTLLQNVRSQIRLASSRKGSRGVVTMAGSGKFFVG 60

Query: 61  GNWKCNGTKESITKLVSDLNNVKLEDDVDVVVAPPFVYIGQVKSSLASRIEISAQNSWVS 120
           GNWKCNGTKESITKLV+DLNN KLEDDVD+VVAPPFVYI QVKSSLASRIEISAQNSWVS
Sbjct: 61  GNWKCNGTKESITKLVADLNNAKLEDDVDIVVAPPFVYIDQVKSSLASRIEISAQNSWVS 120

Query: 121 KGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVIACIGE 180
           KGGAFTGEISVEQLKD+GCKWVILGHSERRHIIGEDDQFIGKKA+YALSEGLGVIACIGE
Sbjct: 121 KGGAFTGEISVEQLKDLGCKWVILGHSERRHIIGEDDQFIGKKASYALSEGLGVIACIGE 180

Query: 181 LLEEREAGKTFDVCFQQLKAFADAVPSWDAVVIAYEPVWAIGTGKVATPEQAQEVHAAVR 240
           LLEEREAGKTFDVCF+QLKAFADAVPSWD +VIAYEPVWAIGTGKVA+PEQAQEVHAAVR
Sbjct: 181 LLEEREAGKTFDVCFRQLKAFADAVPSWDDIVIAYEPVWAIGTGKVASPEQAQEVHAAVR 240

Query: 241 DWLKKNVSPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVT 300
           DWLKKNVSPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVT
Sbjct: 241 DWLKKNVSPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVT 300

Query: 301 AKKVAA 307
           AKKVAA
Sbjct: 301 AKKVAA 306

BLAST of Lag0009267 vs. ExPASy TrEMBL
Match: A0A6J1G6X6 (triosephosphate isomerase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111451334 PE=3 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 8.7e-160
Identity = 292/306 (95.42%), Postives = 300/306 (98.04%), Query Frame = 0

Query: 1   MAVVSTSLASQLSAVRLSSSNSDISHTLFQNVQSQIRLASSRKGSRGVVTMAGSGKFFVG 60
           MAVVSTSLASQLSAVRLS SNSDIS TL QNV+SQIRLASSRKGSRGVVTMAGSGKFFVG
Sbjct: 1   MAVVSTSLASQLSAVRLSGSNSDISQTLLQNVRSQIRLASSRKGSRGVVTMAGSGKFFVG 60

Query: 61  GNWKCNGTKESITKLVSDLNNVKLEDDVDVVVAPPFVYIGQVKSSLASRIEISAQNSWVS 120
           GNWKCNGTKESI+KLV+DLNN KLEDDVDVVVAPPFVYI QVKSSLASRIEISAQNSWVS
Sbjct: 61  GNWKCNGTKESISKLVADLNNAKLEDDVDVVVAPPFVYIDQVKSSLASRIEISAQNSWVS 120

Query: 121 KGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVIACIGE 180
           KGGAFTGEISVEQLKD+GCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGV+ACIGE
Sbjct: 121 KGGAFTGEISVEQLKDLGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVMACIGE 180

Query: 181 LLEEREAGKTFDVCFQQLKAFADAVPSWDAVVIAYEPVWAIGTGKVATPEQAQEVHAAVR 240
           LLEEREAGKTFDVCF+QLKAFADAVPSWD +VIAYEPVWAIGTGKVA+PEQAQEVHAAVR
Sbjct: 181 LLEEREAGKTFDVCFRQLKAFADAVPSWDGIVIAYEPVWAIGTGKVASPEQAQEVHAAVR 240

Query: 241 DWLKKNVSPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVT 300
           DWLKKNVSPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVT
Sbjct: 241 DWLKKNVSPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVT 300

Query: 301 AKKVAA 307
           AKKVAA
Sbjct: 301 AKKVAA 306

BLAST of Lag0009267 vs. ExPASy TrEMBL
Match: A0A6J1FWW5 (triosephosphate isomerase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111448968 PE=3 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 9.6e-159
Identity = 290/306 (94.77%), Postives = 297/306 (97.06%), Query Frame = 0

Query: 1   MAVVSTSLASQLSAVRLSSSNSDISHTLFQNVQSQIRLASSRKGSRGVVTMAGSGKFFVG 60
           MAVVSTSLASQLSA+RLS+S SDIS TLFQNV SQIRLASSRKG RGVVTMAGSGKFFVG
Sbjct: 1   MAVVSTSLASQLSAIRLSTSKSDISQTLFQNVHSQIRLASSRKGPRGVVTMAGSGKFFVG 60

Query: 61  GNWKCNGTKESITKLVSDLNNVKLEDDVDVVVAPPFVYIGQVKSSLASRIEISAQNSWVS 120
           GNWKCNGTKESITKLVSDLNNVKLEDDVDVVVAPPF+YI QV+SSLA RIEISAQNSWVS
Sbjct: 61  GNWKCNGTKESITKLVSDLNNVKLEDDVDVVVAPPFLYIDQVRSSLAPRIEISAQNSWVS 120

Query: 121 KGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLGVIACIGE 180
           KGGAFTGEISVEQLKDIGCKWVILGHSERRH+IGEDDQFIGKKAAYALSEGLGVIACIGE
Sbjct: 121 KGGAFTGEISVEQLKDIGCKWVILGHSERRHVIGEDDQFIGKKAAYALSEGLGVIACIGE 180

Query: 181 LLEEREAGKTFDVCFQQLKAFADAVPSWDAVVIAYEPVWAIGTGKVATPEQAQEVHAAVR 240
           LLEEREAGKTFDVCFQQLKA+ADAV SWD +VIAYEPVWAIGTGKVATPEQAQEVHAAVR
Sbjct: 181 LLEEREAGKTFDVCFQQLKAYADAVSSWDGIVIAYEPVWAIGTGKVATPEQAQEVHAAVR 240

Query: 241 DWLKKNVSPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVT 300
           DWLKKNVSPEVASKTRIIYGGSVNG NCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVT
Sbjct: 241 DWLKKNVSPEVASKTRIIYGGSVNGGNCAELAKKEDIDGFLVGGASLKGPEFGTIVNSVT 300

Query: 301 AKKVAA 307
           AKKVAA
Sbjct: 301 AKKVAA 306

BLAST of Lag0009267 vs. TAIR 10
Match: AT2G21170.1 (triosephosphate isomerase )

HSP 1 Score: 467.6 bits (1202), Expect = 7.6e-132
Identity = 242/315 (76.83%), Postives = 260/315 (82.54%), Query Frame = 0

Query: 1   MAVVSTSLASQLSAVRLSSSNSDIS-----HTLFQNVQSQIRLASSRKGS----RGVVTM 60
           MA  S +     S +R  S   D +      + F  V S  RL SS   S    RGVV M
Sbjct: 1   MAATSLTAPPSFSGLRRISPKLDAAAVSSHQSFFHRVNSSTRLVSSSSSSHRSPRGVVAM 60

Query: 61  AGSGKFFVGGNWKCNGTKESITKLVSDLNNVKLEDDVDVVVAPPFVYIGQVKSSLASRIE 120
           AGSGKFFVGGNWKCNGTK+SI KL+SDLN+  LE DVDVVV+PPFVYI QVKSSL  RI+
Sbjct: 61  AGSGKFFVGGNWKCNGTKDSIAKLISDLNSATLEADVDVVVSPPFVYIDQVKSSLTDRID 120

Query: 121 ISAQNSWVSKGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEG 180
           IS QNSWV KGGAFTGEISVEQLKD+GCKWVILGHSERRH+IGE D+FIGKKAAYALSEG
Sbjct: 121 ISGQNSWVGKGGAFTGEISVEQLKDLGCKWVILGHSERRHVIGEKDEFIGKKAAYALSEG 180

Query: 181 LGVIACIGELLEEREAGKTFDVCFQQLKAFADAVPSWDAVVIAYEPVWAIGTGKVATPEQ 240
           LGVIACIGE LEEREAGKTFDVCF QLKAFADAVPSWD +V+AYEPVWAIGTGKVA+P+Q
Sbjct: 181 LGVIACIGEKLEEREAGKTFDVCFAQLKAFADAVPSWDNIVVAYEPVWAIGTGKVASPQQ 240

Query: 241 AQEVHAAVRDWLKKNVSPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPE 300
           AQEVH AVR WLKKNVS EVASKTRIIYGGSVNG N AELAK+EDIDGFLVGGASLKGPE
Sbjct: 241 AQEVHVAVRGWLKKNVSEEVASKTRIIYGGSVNGGNSAELAKEEDIDGFLVGGASLKGPE 300

Query: 301 FGTIVNSVTAKKVAA 307
           F TIVNSVT+KKVAA
Sbjct: 301 FATIVNSVTSKKVAA 315

BLAST of Lag0009267 vs. TAIR 10
Match: AT2G21170.2 (triosephosphate isomerase )

HSP 1 Score: 437.6 bits (1124), Expect = 8.4e-123
Identity = 233/315 (73.97%), Postives = 251/315 (79.68%), Query Frame = 0

Query: 1   MAVVSTSLASQLSAVRLSSSNSDIS-----HTLFQNVQSQIRLASSRKGS----RGVVTM 60
           MA  S +     S +R  S   D +      + F  V S  RL SS   S    RGVV M
Sbjct: 1   MAATSLTAPPSFSGLRRISPKLDAAAVSSHQSFFHRVNSSTRLVSSSSSSHRSPRGVVAM 60

Query: 61  AGSGKFFVGGNWKCNGTKESITKLVSDLNNVKLEDDVDVVVAPPFVYIGQVKSSLASRIE 120
           AGSGK         NGTK+SI KL+SDLN+  LE DVDVVV+PPFVYI QVKSSL  RI+
Sbjct: 61  AGSGK---------NGTKDSIAKLISDLNSATLEADVDVVVSPPFVYIDQVKSSLTDRID 120

Query: 121 ISAQNSWVSKGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEG 180
           IS QNSWV KGGAFTGEISVEQLKD+GCKWVILGHSERRH+IGE D+FIGKKAAYALSEG
Sbjct: 121 ISGQNSWVGKGGAFTGEISVEQLKDLGCKWVILGHSERRHVIGEKDEFIGKKAAYALSEG 180

Query: 181 LGVIACIGELLEEREAGKTFDVCFQQLKAFADAVPSWDAVVIAYEPVWAIGTGKVATPEQ 240
           LGVIACIGE LEEREAGKTFDVCF QLKAFADAVPSWD +V+AYEPVWAIGTGKVA+P+Q
Sbjct: 181 LGVIACIGEKLEEREAGKTFDVCFAQLKAFADAVPSWDNIVVAYEPVWAIGTGKVASPQQ 240

Query: 241 AQEVHAAVRDWLKKNVSPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPE 300
           AQEVH AVR WLKKNVS EVASKTRIIYGGSVNG N AELAK+EDIDGFLVGGASLKGPE
Sbjct: 241 AQEVHVAVRGWLKKNVSEEVASKTRIIYGGSVNGGNSAELAKEEDIDGFLVGGASLKGPE 300

Query: 301 FGTIVNSVTAKKVAA 307
           F TIVNSVT+KKVAA
Sbjct: 301 FATIVNSVTSKKVAA 306

BLAST of Lag0009267 vs. TAIR 10
Match: AT3G55440.1 (triosephosphate isomerase )

HSP 1 Score: 317.0 bits (811), Expect = 1.7e-86
Identity = 158/252 (62.70%), Postives = 190/252 (75.40%), Query Frame = 0

Query: 56  KFFVGGNWKCNGTKESITKLVSDLNNVKL--EDDVDVVVAPPFVYIGQVKSSLASRIEIS 115
           KFFVGGNWKCNGT E + K+V+ LN  ++  +D V+VVV+PP+V++  VKS+L S   ++
Sbjct: 4   KFFVGGNWKCNGTAEEVKKIVNTLNEAQVPSQDVVEVVVSPPYVFLPLVKSTLRSDFFVA 63

Query: 116 AQNSWVSKGGAFTGEISVEQLKDIGCKWVILGHSERRHIIGEDDQFIGKKAAYALSEGLG 175
           AQN WV KGGAFTGE+S E L ++   WVILGHSERR I+ E  +F+G K AYAL++GL 
Sbjct: 64  AQNCWVKKGGAFTGEVSAEMLVNLDIPWVILGHSERRAILNESSEFVGDKVAYALAQGLK 123

Query: 176 VIACIGELLEEREAGKTFDVCFQQLKAFADAVPSWDAVVIAYEPVWAIGTGKVATPEQAQ 235
           VIAC+GE LEEREAG T DV   Q KA AD V +W  VVIAYEPVWAIGTGKVA+P QAQ
Sbjct: 124 VIACVGETLEEREAGSTMDVVAAQTKAIADRVTNWSNVVIAYEPVWAIGTGKVASPAQAQ 183

Query: 236 EVHAAVRDWLKKNVSPEVASKTRIIYGGSVNGSNCAELAKKEDIDGFLVGGASLKGPEFG 295
           EVH  +R WL KNVS +VA+ TRIIYGGSVNG NC EL  + D+DGFLVGGASLK PEF 
Sbjct: 184 EVHDELRKWLAKNVSADVAATTRIIYGGSVNGGNCKELGGQADVDGFLVGGASLK-PEFI 243

Query: 296 TIVNSVTAKKVA 306
            I+ +   KK A
Sbjct: 244 DIIKAAEVKKSA 254

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022987010.16.2e-16095.42triosephosphate isomerase, chloroplastic-like [Cucurbita maxima][more]
XP_022146193.18.1e-16096.08triosephosphate isomerase, chloroplastic [Momordica charantia][more]
KAG6605006.18.1e-16095.75Triosephosphate isomerase, chloroplastic, partial [Cucurbita argyrosperma subsp.... [more]
XP_023007543.11.4e-15995.42triosephosphate isomerase, chloroplastic-like [Cucurbita maxima][more]
XP_022947479.11.8e-15995.42triosephosphate isomerase, chloroplastic-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
P484962.0e-13778.95Triosephosphate isomerase, chloroplastic OS=Spinacia oleracea OX=3562 GN=TPIP1 P... [more]
Q9M4S81.8e-13378.55Triosephosphate isomerase, chloroplastic OS=Fragaria ananassa OX=3747 GN=TPI PE=... [more]
Q9SKP61.1e-13076.83Triosephosphate isomerase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIM ... [more]
P462251.4e-12282.63Triosephosphate isomerase, chloroplastic OS=Secale cereale OX=4550 PE=1 SV=1[more]
P484973.0e-10171.26Triosephosphate isomerase, cytosolic OS=Stellaria longipes OX=19744 GN=TPI PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A6J1JFM23.0e-16095.42triosephosphate isomerase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1CXY13.9e-16096.08triosephosphate isomerase, chloroplastic OS=Momordica charantia OX=3673 GN=LOC11... [more]
A0A6J1L7Y26.7e-16095.42triosephosphate isomerase, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A6J1G6X68.7e-16095.42triosephosphate isomerase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1FWW59.6e-15994.77triosephosphate isomerase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=L... [more]
Match NameE-valueIdentityDescription
AT2G21170.17.6e-13276.83triosephosphate isomerase [more]
AT2G21170.28.4e-12373.97triosephosphate isomerase [more]
AT3G55440.11.7e-8662.70triosephosphate isomerase [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000652Triosephosphate isomerasePFAMPF00121TIMcoord: 58..296
e-value: 1.3E-90
score: 303.1
IPR000652Triosephosphate isomeraseTIGRFAMTIGR00419TIGR00419coord: 58..288
e-value: 8.5E-81
score: 269.0
IPR000652Triosephosphate isomerasePANTHERPTHR21139TRIOSEPHOSPHATE ISOMERASEcoord: 4..304
IPR000652Triosephosphate isomerasePROSITEPS51440TIM_2coord: 56..298
score: 82.973427
IPR000652Triosephosphate isomeraseCDDcd00311TIMcoord: 57..297
e-value: 3.85374E-131
score: 370.713
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 35..305
e-value: 4.9E-116
score: 388.6
NoneNo IPR availablePANTHERPTHR21139:SF20TRIOSEPHOSPHATE ISOMERASE, CHLOROPLASTICcoord: 4..304
IPR020861Triosephosphate isomerase, active sitePROSITEPS00171TIM_1coord: 214..224
IPR022896Triosephosphate isomerase, bacterial/eukaryoticHAMAPMF_00147_BTIM_Bcoord: 55..298
score: 58.208252
IPR035990Triosephosphate isomerase superfamilySUPERFAMILY51351Triosephosphate isomerase (TIM)coord: 53..298

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0009267.1Lag0009267.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009658 chloroplast organization
biological_process GO:0006094 gluconeogenesis
biological_process GO:0046166 glyceraldehyde-3-phosphate biosynthetic process
biological_process GO:0019563 glycerol catabolic process
biological_process GO:0006096 glycolytic process
biological_process GO:0032504 multicellular organism reproduction
biological_process GO:0080022 primary root development
biological_process GO:0006642 triglyceride mobilization
cellular_component GO:0009507 chloroplast
cellular_component GO:0005829 cytosol
molecular_function GO:0004807 triose-phosphate isomerase activity
molecular_function GO:0003824 catalytic activity