Lag0009185 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0009185
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionAmylomaltase
Locationchr9: 36543375 .. 36546433 (-)
RNA-Seq ExpressionLag0009185
SyntenyLag0009185
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGCAAATTCTTCCTCTCACCAGCGGTGCAAGTTCTTCCTCTCACCAGCGGTGCAAGTTCTTCCTCTCACCATCTCACGTTGTCGCCGGTCATGGGTTTCTTGTGGGGTCGTCGTCGTTAGTCGTGGTTCATCGCCATGGGTCAACNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNCTCGCCATGGGGCGTCGTCGTGGGTAGTAGATTTGAGCCAGCAAAGGTCGTGGGCAGTAGATCTGAGGTCATGGAGGTCGTGGGTTGTTGCCGTAGGAATAAATTCAAAGCTTATGGTCGGAGTTGTTCGAATCTGATCGGTTCTTCATAGATCTGAGTCGTATCTACTTCAATTTTTCAGTTATGAGTACTTCTATTCATTCGGATGTGAAATTTTCAAATTCGATCTATTTTTTTTTTTTTACTTCAATGTGAGTTTCTTAAACTTTTTTTTAGGGTTCCAATGTTGAGATGTTTGTTTTCTTTTTCTAAGTTAGAAAGCTGAAGATCTGTGACTATTTTATGGCTATAGTGAACATTTAAAAAGTTCAAAGCAGAGGATATGAAAATCAACCGTGAAGAGTTTGCTATTCAAAGTAAGGTTTTCTACAATCCTCAATTATCTTTTTTTTTTTTTTTTTTTTGAAGTTCTATTGTAATAGATTTTGTGTCTTAAAACTTTGTTGATGTTGGAGAAAGGGTTTTTGCATTTTCCTTTTCATGATAAAAGAATAATCCTTTGGGCAACCCATTTTTTCTTACTCTTTGGAGTACTTGGCTGGGACTGGGAGGACTTGGAAGGTTCTTAATTAGATGGCAAGAAAGTCTTGATGTGGAGTTCAACATATTTGAGTACCTTCTAATTGGGTGTTTACAACCAAGCATATTTCTTTTTCATTACCTCTAGATTCTAATGTTGTGGATTAAAATCTATTAGATTTTTCTGATGGTTTTTCATGGAGCACTGAATTGTTCCCTTTTCCTGCGTTGTTCCTACGCTCTATGCGACTATGCCATAGAATTATATAATCAATGTGTCAGTTTTTAGTTCCAACTGATAAGAAATTTTTGTCTCTGGTTAAATCCACCAACAATGTTAACTTCTTATCTGCCTAGCTTAGCTGAATATCAGACTAGTCTGATTGACCTTCATATCACTGAAGATATTGTGATTATTGGTTGTTACAGTGCCGTCTTAGAGGGAAGATTGATTCCAATGGTTGTGTAGCTGCTTTCCTTGAAGAGCAACTAAATATGAGTGTTAATTTCATTCTTTCCGCAGAGGTATTTATCAGTTAGCCTTTTTTCCTTTACTAACAAAACAACATGAATCTCTTCAAGTATCTCTATACTTTTATGTTTTTGAAAATGGAAAATTATGCTTATTTCATTTGCAATTTTTCCCTTCCTTCTAGTTTTGAAATTTAGTTCTGAATTTTAAAAGCACTTCTTTAGAAGTAGATAACTGCACAAAAAAATCATGAGGAAATGAGTTTATTTTGTAGAAACAGAAACCTTTCTCTTCACATCTCCAGCAAGTGGGTTTTCGTGGTGGTCGTGGTTCCAGATCTAACTTCATTCGGGTTCATCTCTGTTTGTGGGTATGAATTAGTGGTGGTCATGGCCGTTTGTGCGTAGATCTGAGGTCGTGGGTAGCAAATCTGACATGGTTGAGTCTGATCTGTTTGATTTCTAAGATTTGAGTGGTAAAATTTTTTTGCTTCTTTTTAATTTTATAAAATGTACACTTAAATTTAAAGGGAGAAGCTATAAGGTTTAGAGGGCGCTCCGAAGCCACTGAATCTTCAAGATACTGCCTTCGTTCTACTATAAACGATAAGCCACTGATCATCTCTCTCCAATTTCTGCCATGGAAGCGCTCCGAAGAAGAATTTCTCTCAGCTTGAATCTTCTGTTTCTCTTTTGCGTCTTATTGGTAACAAGTTTCGGTTCCTATCGCGAGCATTACATTTTCTTTTGGATTTTTTTTGTTCTGTTTCTTTTTGCTAGTTGAAATGTGGCAGTGAATTTGTATATGTTTTTCAAGATCCTTTTCATTCGGTACTTCAGCGATTGTGAGCTGAATTCCTCCGTGACCTCGATGTGGTCTTCAAATTACCTTTTCTAGCGGCCTTTGGAGTAGATTTCTGTAGATTGATTCCGATTGTTGTGATAGTTGTTGGATATTTCTTTTTTACTTATGTATCTGTTAGAGATTTGATTTCGCCTAAGTGAAACGTACCAGACTGTTGACTCTGATTTTAAGTTTGTACTTCTGTTGTGTAGCAAGCTTTGCTCTGCTTGTATCTTTATTTTCATAGTTTATAAGATTTCATTCCAAGATTAGATCGTAGTTTTTGGATATATCAATTTTATCGTCTGTCCTTATACTTTGTTGGCCATATATTAACCTTATTCATATATAATTTTCATGTTTGAATTATAGATGTCAAACTACTTCACAGCTTATAGAATTGATCATATATTGGGTTTCTTTCGAATTTGGGAGCTTCCAGAGCATGTCGTGACTGGTTTAGTTGGAAAATTCCGCCCTTCTATTCCTTTAAGCCAGGTACGTTTGCTTGTTTCAAAATCAAACATTTTTATTTGAAGTGTGAATAGTTGTGAATTTTAAAAGTTATTTGTCCACAGGAATAACTTGAAAGAGAGGGGATATGGGACTTTGATCGCTTGAGTCGTCCATATATCAAGGCTGAATTTTTAAAGGTCGCTACATTATGAATCTAATTGGCAATCCTGGAAAGCATTATTTGATTCTGATGCCCTGAAGTACGTTCTTCCTTTTATTTTTTCAGGATAAATTTGGAGCTGCATGGGGATTTATTGCGTCACACTTTCTTAATGAATACCAGAAGAACTTTTATGAGGTGATACTTAAATTATGATGCATCACATTGATTTGCATAAAATTGTATCTTCAGTTGTCCATGGTATCCCATGAGAACACTATTGTGCTTGTTGAATACTGA

mRNA sequence

ATGACGCAAATTCTTCCTCTCACCAGCGGTGCAAGTTCTTCCTCTCACCAGCGGTGCAAGTTCTTCCTCTCACCATCTCACGTTGTCGCCGGTCATGGGTTTCTTGTGGGGTCGTCGTCGTTAGTCGTGTGCCGTCTTAGAGGGAAGATTGATTCCAATGGTTGTGTAGCTGCTTTCCTTGAAGAGCAACTAAATATGAGTGTTAATTTCATTCTTTCCGCAGAGATGTCAAACTACTTCACAGCTTATAGAATTGATCATATATTGGGTTTCTTTCGAATTTGGGAGCTTCCAGAGCATGTCGTGACTGGTTTAGTTGGAAAATTCCGCCCTTCTATTCCTTTAAGCCAGGATAAATTTGGAGCTGCATGGGGATTTATTGCGTCACACTTTCTTAATGAATACCAGAAGAACTTTTATGAGTTGTCCATGGTATCCCATGAGAACACTATTGTGCTTGTTGAATACTGA

Coding sequence (CDS)

ATGACGCAAATTCTTCCTCTCACCAGCGGTGCAAGTTCTTCCTCTCACCAGCGGTGCAAGTTCTTCCTCTCACCATCTCACGTTGTCGCCGGTCATGGGTTTCTTGTGGGGTCGTCGTCGTTAGTCGTGTGCCGTCTTAGAGGGAAGATTGATTCCAATGGTTGTGTAGCTGCTTTCCTTGAAGAGCAACTAAATATGAGTGTTAATTTCATTCTTTCCGCAGAGATGTCAAACTACTTCACAGCTTATAGAATTGATCATATATTGGGTTTCTTTCGAATTTGGGAGCTTCCAGAGCATGTCGTGACTGGTTTAGTTGGAAAATTCCGCCCTTCTATTCCTTTAAGCCAGGATAAATTTGGAGCTGCATGGGGATTTATTGCGTCACACTTTCTTAATGAATACCAGAAGAACTTTTATGAGTTGTCCATGGTATCCCATGAGAACACTATTGTGCTTGTTGAATACTGA

Protein sequence

MTQILPLTSGASSSSHQRCKFFLSPSHVVAGHGFLVGSSSLVVCRLRGKIDSNGCVAAFLEEQLNMSVNFILSAEMSNYFTAYRIDHILGFFRIWELPEHVVTGLVGKFRPSIPLSQDKFGAAWGFIASHFLNEYQKNFYELSMVSHENTIVLVEY
Homology
[Close]
BLAST of Lag0009185 vs. NCBI nr
Match: XP_004144319.1 (4-alpha-glucanotransferase DPE2 [Cucumis sativus])

HSP 1 Score: 129.8 bits (325), Expect = 2.0e-26
Identity = 64/92 (69.57%), Postives = 66/92 (71.74%), Query Frame = 0 

Query: 75  EMSNYFTAYRIDHILGFFRIWELPEHVVTGLVGKFRPSIPLS------------------ 134
           +MSNYFTAYRIDHILGFFRIWELPEH +TGLVGKFRPSIPLS                  
Sbjct: 549 QMSNYFTAYRIDHILGFFRIWELPEHAMTGLVGKFRPSIPLSQEELEREGIWDFDRLSRP 608

Query: 135 -------QDKFGAAWGFIASHFLNEYQKNFYE 142
                  QDKFGAAWGFIASHFLNEYQKNFYE
Sbjct: 609 YIKAEFLQDKFGAAWGFIASHFLNEYQKNFYE 640

[Close]
BLAST of Lag0009185 vs. NCBI nr
Match: KGN54878.2 (hypothetical protein Csa_012907 [Cucumis sativus])

HSP 1 Score: 129.8 bits (325), Expect = 2.0e-26
Identity = 64/92 (69.57%), Postives = 66/92 (71.74%), Query Frame = 0 

Query: 75   EMSNYFTAYRIDHILGFFRIWELPEHVVTGLVGKFRPSIPLS------------------ 134
            +MSNYFTAYRIDHILGFFRIWELPEH +TGLVGKFRPSIPLS                  
Sbjct: 1052 QMSNYFTAYRIDHILGFFRIWELPEHAMTGLVGKFRPSIPLSQEELEREGIWDFDRLSRP 1111

Query: 135  -------QDKFGAAWGFIASHFLNEYQKNFYE 142
                   QDKFGAAWGFIASHFLNEYQKNFYE
Sbjct: 1112 YIKAEFLQDKFGAAWGFIASHFLNEYQKNFYE 1143

[Close]
BLAST of Lag0009185 vs. NCBI nr
Match: XP_016901815.1 (PREDICTED: 4-alpha-glucanotransferase DPE2 isoform X2 [Cucumis melo])

HSP 1 Score: 129.8 bits (325), Expect = 2.0e-26
Identity = 64/92 (69.57%), Postives = 66/92 (71.74%), Query Frame = 0 

Query: 75  EMSNYFTAYRIDHILGFFRIWELPEHVVTGLVGKFRPSIPLS------------------ 134
           +MSNYFTAYRIDHILGFFRIWELPEH +TGLVGKFRPSIPLS                  
Sbjct: 549 QMSNYFTAYRIDHILGFFRIWELPEHAMTGLVGKFRPSIPLSQEELEREGIWDFDRLSRP 608

Query: 135 -------QDKFGAAWGFIASHFLNEYQKNFYE 142
                  QDKFGAAWGFIASHFLNEYQKNFYE
Sbjct: 609 YIKAEFLQDKFGAAWGFIASHFLNEYQKNFYE 640

[Close]
BLAST of Lag0009185 vs. NCBI nr
Match: XP_008455750.1 (PREDICTED: 4-alpha-glucanotransferase DPE2 isoform X1 [Cucumis melo])

HSP 1 Score: 129.8 bits (325), Expect = 2.0e-26
Identity = 64/92 (69.57%), Postives = 66/92 (71.74%), Query Frame = 0 

Query: 75  EMSNYFTAYRIDHILGFFRIWELPEHVVTGLVGKFRPSIPLS------------------ 134
           +MSNYFTAYRIDHILGFFRIWELPEH +TGLVGKFRPSIPLS                  
Sbjct: 549 QMSNYFTAYRIDHILGFFRIWELPEHAMTGLVGKFRPSIPLSQEELEREGIWDFDRLSRP 608

Query: 135 -------QDKFGAAWGFIASHFLNEYQKNFYE 142
                  QDKFGAAWGFIASHFLNEYQKNFYE
Sbjct: 609 YIKAEFLQDKFGAAWGFIASHFLNEYQKNFYE 640

[Close]
BLAST of Lag0009185 vs. NCBI nr
Match: TYK09640.1 (4-alpha-glucanotransferase DPE2 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 129.8 bits (325), Expect = 2.0e-26
Identity = 64/92 (69.57%), Postives = 66/92 (71.74%), Query Frame = 0 

Query: 75  EMSNYFTAYRIDHILGFFRIWELPEHVVTGLVGKFRPSIPLS------------------ 134
           +MSNYFTAYRIDHILGFFRIWELPEH +TGLVGKFRPSIPLS                  
Sbjct: 286 QMSNYFTAYRIDHILGFFRIWELPEHAMTGLVGKFRPSIPLSQEELEREGIWDFDRLSRP 345

Query: 135 -------QDKFGAAWGFIASHFLNEYQKNFYE 142
                  QDKFGAAWGFIASHFLNEYQKNFYE
Sbjct: 346 YIKAEFLQDKFGAAWGFIASHFLNEYQKNFYE 377

[Close]
BLAST of Lag0009185 vs. ExPASy Swiss-Prot
Match: Q8RXD9 (4-alpha-glucanotransferase DPE2 OS=Arabidopsis thaliana OX=3702 GN=DPE2 PE=1 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 1.6e-21
Identity = 53/92 (57.61%), Postives = 58/92 (63.04%), Query Frame = 0 

Query: 75  EMSNYFTAYRIDHILGFFRIWELPEHVVTGLVGKFRPSIPLSQ----------------- 134
           +M  YFTAYRIDHILGFFRIWELP H +TGLVGKFRPSIPLSQ                 
Sbjct: 552 QMGKYFTAYRIDHILGFFRIWELPAHAMTGLVGKFRPSIPLSQEELEKEGIWDFDRLSKP 611

Query: 135 --------DKFGAAWGFIASHFLNEYQKNFYE 142
                   +KFG  W FIAS+FLNE QK+ YE
Sbjct: 612 YIQKKFLEEKFGDFWPFIASNFLNETQKDMYE 643

[Close]
BLAST of Lag0009185 vs. ExPASy Swiss-Prot
Match: Q69Q02 (4-alpha-glucanotransferase DPE2 OS=Oryza sativa subsp. japonica OX=39947 GN=DPE2 PE=2 SV=1)

HSP 1 Score: 100.5 bits (249), Expect = 1.7e-20
Identity = 50/92 (54.35%), Postives = 58/92 (63.04%), Query Frame = 0 

Query: 75  EMSNYFTAYRIDHILGFFRIWELPEHVVTGLVGKFRPSIPLSQ----------------- 134
           +M+ YFTAYRIDHILGFFRIWELP+H  TGLVGKFRPSI LSQ                 
Sbjct: 546 QMAKYFTAYRIDHILGFFRIWELPDHAATGLVGKFRPSIALSQEELLSEGLWDFDRMSRP 605

Query: 135 --------DKFGAAWGFIASHFLNEYQKNFYE 142
                   +KFG+ W  IA++FLNEY+K  YE
Sbjct: 606 YILQETLEEKFGSFWTVIAANFLNEYKKQHYE 637

[Close]
BLAST of Lag0009185 vs. ExPASy Swiss-Prot
Match: Q9SX55 (Probable mitochondrial import receptor subunit TOM40-2 OS=Arabidopsis thaliana OX=3702 GN=TOM40-2 PE=1 SV=3)

HSP 1 Score: 49.7 bits (117), Expect = 3.5e-05
Identity = 23/40 (57.50%), Postives = 31/40 (77.50%), Query Frame = 0 

Query: 45  RLRGKIDSNGCVAAFLEEQLNMSVNFILSAEMSNYFTAYR 85
           RLRGK+DSNG VAA+LEEQL + + F+LSAE+ +    Y+
Sbjct: 263 RLRGKVDSNGVVAAYLEEQLPIGLRFLLSAEVDHVKKDYK 302

[Close]
BLAST of Lag0009185 vs. ExPASy Swiss-Prot
Match: Q9LHE5 (Mitochondrial import receptor subunit TOM40-1 OS=Arabidopsis thaliana OX=3702 GN=TOM40-1 PE=1 SV=3)

HSP 1 Score: 48.5 bits (114), Expect = 7.9e-05
Identity = 22/40 (55.00%), Postives = 31/40 (77.50%), Query Frame = 0 

Query: 45  RLRGKIDSNGCVAAFLEEQLNMSVNFILSAEMSNYFTAYR 85
           R+RGKIDSNG  +A LEE+L+M +NF+LSAE+ +    Y+
Sbjct: 262 RVRGKIDSNGVASALLEERLSMGLNFLLSAELDHKKKDYK 301

The following BLAST results are available for this feature:
BLAST of Lag0009185 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_004144319.12.0e-2669.574-alpha-glucanotransferase DPE2 [Cucumis sativus][more]
KGN54878.22.0e-2669.57hypothetical protein Csa_012907 [Cucumis sativus][more]
XP_016901815.12.0e-2669.57PREDICTED: 4-alpha-glucanotransferase DPE2 isoform X2 [Cucumis melo][more]
XP_008455750.12.0e-2669.57PREDICTED: 4-alpha-glucanotransferase DPE2 isoform X1 [Cucumis melo][more]
TYK09640.12.0e-2669.574-alpha-glucanotransferase DPE2 isoform X1 [Cucumis melo var. makuwa][more]
BLAST of Lag0009185 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q8RXD91.6e-2157.614-alpha-glucanotransferase DPE2 OS=Arabidopsis thaliana OX=3702 GN=DPE2 PE=1 SV=... [more]
Q69Q021.7e-2054.354-alpha-glucanotransferase DPE2 OS=Oryza sativa subsp. japonica OX=39947 GN=DPE2... [more]
Q9SX553.5e-0557.50Probable mitochondrial import receptor subunit TOM40-2 OS=Arabidopsis thaliana O... [more]
Q9LHE57.9e-0555.00Mitochondrial import receptor subunit TOM40-1 OS=Arabidopsis thaliana OX=3702 GN... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003385Glycoside hydrolase, family 77PFAMPF02446Glyco_hydro_77coord: 75..100
e-value: 7.6E-8
score: 31.6
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 71..123
e-value: 7.2E-7
score: 30.1
NoneNo IPR availablePANTHERPTHR32518FAMILY NOT NAMEDcoord: 75..143
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 76..112

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0009185.1Lag0009185.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005977 glycogen metabolic process
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004134 4-alpha-glucanotransferase activity
molecular_function GO:0102500 beta-maltose 4-alpha-glucanotransferase activity
molecular_function GO:0004175 endopeptidase activity
molecular_function GO:2001070 starch binding