Lag0009171 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0009171
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	AB hydrolase-1 domain-containing protein
Location	chr9: 36417073 .. 36418007 (-)
RNA-Seq Expression	Lag0009171
Synteny	Lag0009171

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTGAACATTTTCCACCTCTACGACCGGGTTCTACGGTGGCTAATGAAGGCGACCGGAATCAGACCGGAAATGGTCCAGATCGAACCGGGAACCACCATGAATTTCTGGGTTCCAACCAAAAACAATAACCCAAATCCTCCCGTGGTTCTTCTCCACGGATTCGCCGCCAACGCGATCTTGACGTGGCAGTTTCAGGTGCTGAGTCTGGCCAAAAACCACGCCGTTTACGTACCGGACTTCATCTTCTTCGGCGACTCCGTGACGGACCGGCCCGAGAGGTCGCCGGAGTTTCAGGCGGAGTGCGTGGCGGAGGGGCTGAGGAAGCTCGGCGTCGAGAGGCCGTGCGTTTTCGTGGGGTTTAGCTATGGGGCCATGGTGGGGTTCAGGCTGGCTCAGATGTACCCCGATTTGGTGGCGGCGATGGTGGTGACGGCGGCGCCGACGGTGCTGACGGCGTCGCTGAGCCGTGGGTTGCTGGAGAAGATTGGGTTTGAGAGTTGGTCGGATTATTTGATTCCGGCGACGGTGAAGGGGGTTCGGTGCATGTTTGAAATCGGTTCATTTAGGGCCCGTTTGGTAACGTTCTTGTTTCTTGTTTCCGTTTCTTCATTTTTTAGAAAGAAAAATAGAATTTCATGACCCTAACACCTATACATTCAATAATAATAAACCTATATTTTTAGTTATGTTTGGAAAAAAATTGGAAACAAGAACAAAAAATGGGAAACTAGTTTTAGTTGTTTCCAAAAGTTCTATACAAATTTTGGGAGTGTTTTTAAAAAATGAGAAACAAAAAATATACATAGTTATTATATGCTTAGATTTCTTAATAAAATTAGAAACAAGAAACAAGAAACGAGAACGTTATCAAACAGGCCCTTAAGTTGCCGGCGGTTCCTGATTGGATTTTCAAACACTATTTGGAGGTATGA

mRNA sequence

ATGGTGAACATTTTCCACCTCTACGACCGGGTTCTACGGTGGCTAATGAAGGCGACCGGAATCAGACCGGAAATGGTCCAGATCGAACCGGGAACCACCATGAATTTCTGGGTTCCAACCAAAAACAATAACCCAAATCCTCCCGTGGTTCTTCTCCACGGATTCGCCGCCAACGCGATCTTGACGTGGCAGTTTCAGGTGCTGAGTCTGGCCAAAAACCACGCCGTTTACGTACCGGACTTCATCTTCTTCGGCGACTCCGTGACGGACCGGCCCGAGAGGTCGCCGGAGTTTCAGGCGGAGTGCGTGGCGGAGGGGCTGAGGAAGCTCGGCGTCGAGAGGCCGTGCGTTTTCGTGGGGTTTAGCTATGGGGCCATGGTGGGGTTCAGGCTGGCTCAGATGTACCCCGATTTGGTGGCGGCGATGGTGGTGACGGCGGCGCCGACGGTGCTGACGGCGTCGCTGAGCCGTGGGTTGCTGGAGAAGATTGGGTTTGAGAGTTGGTCGGATTATTTGATTCCGGCGACGGTGAAGGGGGTTCGGTGCATGTTTGAAATCGGTTCATTTAGGGCCCGTTTGGCCCTTAAGTTGCCGGCGGTTCCTGATTGGATTTTCAAACACTATTTGGAGGTATGA

Coding sequence (CDS)

ATGGTGAACATTTTCCACCTCTACGACCGGGTTCTACGGTGGCTAATGAAGGCGACCGGAATCAGACCGGAAATGGTCCAGATCGAACCGGGAACCACCATGAATTTCTGGGTTCCAACCAAAAACAATAACCCAAATCCTCCCGTGGTTCTTCTCCACGGATTCGCCGCCAACGCGATCTTGACGTGGCAGTTTCAGGTGCTGAGTCTGGCCAAAAACCACGCCGTTTACGTACCGGACTTCATCTTCTTCGGCGACTCCGTGACGGACCGGCCCGAGAGGTCGCCGGAGTTTCAGGCGGAGTGCGTGGCGGAGGGGCTGAGGAAGCTCGGCGTCGAGAGGCCGTGCGTTTTCGTGGGGTTTAGCTATGGGGCCATGGTGGGGTTCAGGCTGGCTCAGATGTACCCCGATTTGGTGGCGGCGATGGTGGTGACGGCGGCGCCGACGGTGCTGACGGCGTCGCTGAGCCGTGGGTTGCTGGAGAAGATTGGGTTTGAGAGTTGGTCGGATTATTTGATTCCGGCGACGGTGAAGGGGGTTCGGTGCATGTTTGAAATCGGTTCATTTAGGGCCCGTTTGGCCCTTAAGTTGCCGGCGGTTCCTGATTGGATTTTCAAACACTATTTGGAGGTATGA

Protein sequence

MVNIFHLYDRVLRWLMKATGIRPEMVQIEPGTTMNFWVPTKNNNPNPPVVLLHGFAANAILTWQFQVLSLAKNHAVYVPDFIFFGDSVTDRPERSPEFQAECVAEGLRKLGVERPCVFVGFSYGAMVGFRLAQMYPDLVAAMVVTAAPTVLTASLSRGLLEKIGFESWSDYLIPATVKGVRCMFEIGSFRARLALKLPAVPDWIFKHYLEV

Homology

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BLAST of Lag0009171 vs. NCBI nr
Match: KAG6605077.1 (Epoxide hydrolase 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 340.1 bits (871), Expect = 1.4e-89
Identity = 160/212 (75.47%), Postives = 183/212 (86.32%), Query Frame = 0


Query: 1   MVNIFHLYDRVLRWLMKATGIRPEMVQIEPGTTMNFWVPTKN--NNPNPPVVLLHGFAAN 60
           M NIFH+Y RVLRWLMK  G+R E+VQIEPGTTMNFWVPT+N   NPNPPVVLLHGFA N
Sbjct: 1   MANIFHVYHRVLRWLMKVAGVRQELVQIEPGTTMNFWVPTRNPIKNPNPPVVLLHGFAGN 60

Query: 61  AILTWQFQVLSLAKNHAVYVPDFIFFGDSVTDRPERSPEFQAECVAEGLRKLGVERPCVF 120
            I+TWQFQV++LA+N+AVYVPDF+FFG+SVTDR ERSPEFQAECV +GLR+LGVERPCV 
Sbjct: 61  GIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAECVVKGLRRLGVERPCVL 120

Query: 121 VGFSYGAMVGFRLAQMYPDLVAAMVVTAAPTVLTASLSRGLLEKIGFESWSDYLIPATVK 180
           VGFSYGAMVGFRLA+MYPDLVAAMVVT+APT LT SLSR  L++IG++SWSDYLIP TV 
Sbjct: 121 VGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTALTESLSRVALQEIGYKSWSDYLIPNTVN 180

Query: 181 GVRCMFEIGSFRARLALKLPAVPDWIFKHYLE 211
           GV+ +FEIGSF        PA+P+WI KHYLE
Sbjct: 181 GVKSLFEIGSF------DFPALPNWIMKHYLE 206

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BLAST of Lag0009171 vs. NCBI nr
Match: XP_023533135.1 (epoxide hydrolase 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 340.1 bits (871), Expect = 1.4e-89
Identity = 160/212 (75.47%), Postives = 183/212 (86.32%), Query Frame = 0


Query: 1   MVNIFHLYDRVLRWLMKATGIRPEMVQIEPGTTMNFWVPTKN--NNPNPPVVLLHGFAAN 60
           M NIFH+Y RVLRWLMK  G+R E+VQI+PGTTMNFWVPT+N   NPNPPVVLLHGFA N
Sbjct: 1   MANIFHVYHRVLRWLMKVAGVRQELVQIQPGTTMNFWVPTRNPKKNPNPPVVLLHGFAGN 60

Query: 61  AILTWQFQVLSLAKNHAVYVPDFIFFGDSVTDRPERSPEFQAECVAEGLRKLGVERPCVF 120
            I+TWQFQV++LA+N+AVYVPDF+FFG+SVTDR ERSPEFQAECV +GLRKLGVERPCV 
Sbjct: 61  GIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAECVVKGLRKLGVERPCVL 120

Query: 121 VGFSYGAMVGFRLAQMYPDLVAAMVVTAAPTVLTASLSRGLLEKIGFESWSDYLIPATVK 180
           VGFSYGAMVGFRLA+MYPDLVAAMVVT+APT LT SLSR  L++IG++SWSDYLIP TV 
Sbjct: 121 VGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTALTESLSRVALQEIGYKSWSDYLIPNTVN 180

Query: 181 GVRCMFEIGSFRARLALKLPAVPDWIFKHYLE 211
           GV+ +FEIGSF        PA+P+WI KHYLE
Sbjct: 181 GVKSLFEIGSF------DFPALPNWIMKHYLE 206

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BLAST of Lag0009171 vs. NCBI nr
Match: KAG7035085.1 (Epoxide hydrolase 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 338.6 bits (867), Expect = 3.9e-89
Identity = 159/212 (75.00%), Postives = 183/212 (86.32%), Query Frame = 0


Query: 1   MVNIFHLYDRVLRWLMKATGIRPEMVQIEPGTTMNFWVPTKN--NNPNPPVVLLHGFAAN 60
           M NIFH+Y RVLRWLMK  G+R E+VQIEPGTTMNFWVPT+N   NPNPPVVLLHGFA N
Sbjct: 6   MANIFHVYHRVLRWLMKVAGVRQELVQIEPGTTMNFWVPTRNPKKNPNPPVVLLHGFAGN 65

Query: 61  AILTWQFQVLSLAKNHAVYVPDFIFFGDSVTDRPERSPEFQAECVAEGLRKLGVERPCVF 120
            I+TWQFQV++LA+N+AVYVPDF+FFG+SVTDR ERSPEFQAECV +GLR+LGVERPCV 
Sbjct: 66  GIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAECVVKGLRRLGVERPCVL 125

Query: 121 VGFSYGAMVGFRLAQMYPDLVAAMVVTAAPTVLTASLSRGLLEKIGFESWSDYLIPATVK 180
           VGFSYGAMVGFRLA+MYPDLVAAMVVT+APT LT SLSR  L++IG++SWSD+LIP TV 
Sbjct: 126 VGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTALTESLSRVALQEIGYKSWSDFLIPNTVN 185

Query: 181 GVRCMFEIGSFRARLALKLPAVPDWIFKHYLE 211
           GV+ +FEIGSF        PA+P+WI KHYLE
Sbjct: 186 GVKSLFEIGSF------DFPALPNWIMKHYLE 211

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BLAST of Lag0009171 vs. NCBI nr
Match: XP_023006837.1 (epoxide hydrolase 4-like [Cucurbita maxima])

HSP 1 Score: 336.7 bits (862), Expect = 1.5e-88
Identity = 158/213 (74.18%), Postives = 185/213 (86.85%), Query Frame = 0


Query: 1   MVNIFHLYDRVLRWLMKATGIRPEMVQIEPGTTMNFWVPTKN--NNPNPPVVLLHGFAAN 60
           M NIFH+Y RVLRWLMK  G+R E+VQI+PGTTMNFWVPT+N   NPNPPVVLLHGFA N
Sbjct: 1   MANIFHVYHRVLRWLMKVAGVRQELVQIQPGTTMNFWVPTRNPKKNPNPPVVLLHGFAGN 60

Query: 61  AILTWQFQVLSLAKNHAVYVPDFIFFGDSVTDRPERSPEFQAECVAEGLRKLGVERPCVF 120
            I+TWQFQV++LA+N+AVYVPDF+FFG+SVTDR ERSPEFQAECV +GLR+LGVERPCV 
Sbjct: 61  GIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAECVVKGLRRLGVERPCVL 120

Query: 121 VGFSYGAMVGFRLAQMYPDLVAAMVVTAAPTVLTASLSRGLLEKIGFESWSDYLIPATVK 180
           VGFSYGAMVGFRLA+MYPDLVAAMVVT+APTVLT SLSR  L++IG++SWSDYLIP TVK
Sbjct: 121 VGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTVLTESLSREALQEIGYKSWSDYLIPNTVK 180

Query: 181 GVRCMFEIGSFRARLALKLPAVPDWIFKHYLEV 212
           GV+ +FEIGSF        PA+P+WI + Y++V
Sbjct: 181 GVKSLFEIGSF------DFPALPNWIMEQYVKV 207

[Close]

BLAST of Lag0009171 vs. NCBI nr
Match: XP_022947943.1 (epoxide hydrolase 4-like [Cucurbita moschata])

HSP 1 Score: 331.3 bits (848), Expect = 6.3e-87
Identity = 159/212 (75.00%), Postives = 180/212 (84.91%), Query Frame = 0


Query: 1   MVNIFHLYDRVLRWLMKATGIRPEMVQIEPGTTMNFWVPTKN--NNPNPPVVLLHGFAAN 60
           M NIFH+Y RVLRWLMK  G+R E+VQIEPGTTMNFWVPT+N   N NPPVVLLHGFA N
Sbjct: 1   MANIFHVYHRVLRWLMKVAGVRQELVQIEPGTTMNFWVPTRNPKKNRNPPVVLLHGFAGN 60

Query: 61  AILTWQFQVLSLAKNHAVYVPDFIFFGDSVTDRPERSPEFQAECVAEGLRKLGVERPCVF 120
            I+TWQFQV++LA+N+AVYVPDF+FFG+SVTDR ERSPEFQAE V +GLRKLGVERPCV 
Sbjct: 61  GIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAEGVVKGLRKLGVERPCVL 120

Query: 121 VGFSYGAMVGFRLAQMYPDLVAAMVVTAAPTVLTASLSRGLLEKIGFESWSDYLIPATVK 180
           VGFSYGAMVGFRLA+MYPDLVAAMVVT+APT LT SLSR  L +IG++SWSDYLIP TV 
Sbjct: 121 VGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTALTESLSRVALLEIGYKSWSDYLIPNTVN 180

Query: 181 GVRCMFEIGSFRARLALKLPAVPDWIFKHYLE 211
           GV+ +FEIGSF        PA+P+WI KHYLE
Sbjct: 181 GVKSLFEIGSF------DFPALPNWIMKHYLE 206

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BLAST of Lag0009171 vs. ExPASy Swiss-Prot
Match: Q9A919 (Haloalkane dehalogenase OS=Caulobacter vibrioides (strain ATCC 19089 / CB15) OX=190650 GN=dhmA PE=1 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 8.2e-05
Identity = 36/145 (24.83%), Postives = 68/145 (46.90%), Query Frame = 0


Query: 31  GTTMNFWVPTKNNNPNPPVVLLHGFAANAILTWQFQVLSLAKNHAVYVPDFIFFGDSVTD 90
           GT +      +      P++L+HG  + A L  +     +AK H V  PD + FG S  D
Sbjct: 31  GTALRIHHVDEGPKDQRPILLMHGEPSWAYLYRKVIAELVAKGHRVVAPDLVGFGRS--D 90

Query: 91  RPERSPEFQAE----CVAEGLRKLGVERPCVFVGFSYGAMVGFRLAQMYPDLVAAMVVTA 150
           +P +  ++  E     ++  L +  ++   +F    +G ++G RL   +P+  +A+VV+ 
Sbjct: 91  KPAKRTDYTYERHVAWMSAWLEQNDLKDIVLFCQ-DWGGLIGLRLVAAFPERFSAVVVS- 150

Query: 151 APTVLTASLSRGLLEKIGFESWSDY 172
                   L  G+ +  GFE+W ++
Sbjct: 151 -----NTGLPIGVGKSEGFEAWLNF 166

[Close]

BLAST of Lag0009171 vs. ExPASy Swiss-Prot
Match: B8H3S9 (Haloalkane dehalogenase OS=Caulobacter vibrioides (strain NA1000 / CB15N) OX=565050 GN=dhmA PE=3 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 8.2e-05
Identity = 36/145 (24.83%), Postives = 68/145 (46.90%), Query Frame = 0


Query: 31  GTTMNFWVPTKNNNPNPPVVLLHGFAANAILTWQFQVLSLAKNHAVYVPDFIFFGDSVTD 90
           GT +      +      P++L+HG  + A L  +     +AK H V  PD + FG S  D
Sbjct: 31  GTALRIHHVDEGPKDQRPILLMHGEPSWAYLYRKVIAELVAKGHRVVAPDLVGFGRS--D 90

Query: 91  RPERSPEFQAE----CVAEGLRKLGVERPCVFVGFSYGAMVGFRLAQMYPDLVAAMVVTA 150
           +P +  ++  E     ++  L +  ++   +F    +G ++G RL   +P+  +A+VV+ 
Sbjct: 91  KPAKRTDYTYERHVAWMSAWLEQNDLKDIVLFCQ-DWGGLIGLRLVAAFPERFSAVVVS- 150

Query: 151 APTVLTASLSRGLLEKIGFESWSDY 172
                   L  G+ +  GFE+W ++
Sbjct: 151 -----NTGLPIGVGKSEGFEAWLNF 166

[Close]

BLAST of Lag0009171 vs. ExPASy Swiss-Prot
Match: P34913 (Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2)

HSP 1 Score: 48.1 bits (113), Expect = 1.4e-04
Identity = 51/191 (26.70%), Postives = 81/191 (42.41%), Query Frame = 0


Query: 9   DRVLRWLMKATGIR------PEMVQIEPGTTMNFWVPTKNN--------NPNPPVVLLHG 68
           D  L+ L K TGI+      P      P    + +V  K             P V L HG
Sbjct: 207 DTALKELEKVTGIQLLNTPAPLPTSCNPSDMSHGYVTVKPRVRLHFVELGSGPAVCLCHG 266

Query: 69  FAANAILTWQFQVLSLAK-NHAVYVPDFIFFGDSVTDRPERSPEFQAECVAEG----LRK 128
           F   +  +W++Q+ +LA+  + V   D   +G+S    P    E+  E + +     L K
Sbjct: 267 F-PESWYSWRYQIPALAQAGYRVLAMDMKGYGES--SAPPEIEEYCMEVLCKEMVTFLDK 326

Query: 129 LGVERPCVFVGFSYGAMVGFRLAQMYPDLVAAMVVTAAPTVLTASLSRGLLEKIGFESWS 181
           LG+ +  VF+G  +G M+ + +A  YP+ V A+     P  + A+ +   LE I      
Sbjct: 327 LGLSQ-AVFIGHDWGGMLVWYMALFYPERVRAVASLNTP-FIPANPNMSPLESIKANPVF 386

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BLAST of Lag0009171 vs. ExPASy Swiss-Prot
Match: B0SY51 (Haloalkane dehalogenase OS=Caulobacter sp. (strain K31) OX=366602 GN=dhmA PE=3 SV=1)

HSP 1 Score: 47.8 bits (112), Expect = 1.8e-04
Identity = 39/133 (29.32%), Postives = 60/133 (45.11%), Query Frame = 0


Query: 48  PVVLLHGFAANAILTWQFQVLSLAKNHAVYVPDFIFFGDSVTDRPERSPEFQAE------ 107
           PV+L+HG  + A L  +      A+ H V  PD I FG S  D+P    ++  E      
Sbjct: 48  PVLLMHGEPSWAYLYRKIIPALAARGHRVIAPDLIGFGRS--DKPAARGDYTYERHVAWM 107

Query: 108 -CVAEGLRKLGVERPCVFVGFSYGAMVGFRLAQMYPDLVAAMVV--TAAPTVLTASLSRG 167
               EGL   G+   C      +G ++G RL   +P+  A +V+  T  PT        G
Sbjct: 108 SAWLEGLDLRGLTLFCQ----DWGGLIGLRLVAAFPERFAGLVIANTGLPT------GAG 166

Query: 168 LLEKIGFESWSDY 172
           + +  GF++W D+
Sbjct: 168 MTD--GFKAWLDF 166

[Close]

BLAST of Lag0009171 vs. ExPASy TrEMBL
Match: A0A6J1KWX2 (epoxide hydrolase 4-like OS=Cucurbita maxima OX=3661 GN=LOC111499509 PE=4 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 7.2e-89
Identity = 158/213 (74.18%), Postives = 185/213 (86.85%), Query Frame = 0


Query: 1   MVNIFHLYDRVLRWLMKATGIRPEMVQIEPGTTMNFWVPTKN--NNPNPPVVLLHGFAAN 60
           M NIFH+Y RVLRWLMK  G+R E+VQI+PGTTMNFWVPT+N   NPNPPVVLLHGFA N
Sbjct: 1   MANIFHVYHRVLRWLMKVAGVRQELVQIQPGTTMNFWVPTRNPKKNPNPPVVLLHGFAGN 60

Query: 61  AILTWQFQVLSLAKNHAVYVPDFIFFGDSVTDRPERSPEFQAECVAEGLRKLGVERPCVF 120
            I+TWQFQV++LA+N+AVYVPDF+FFG+SVTDR ERSPEFQAECV +GLR+LGVERPCV 
Sbjct: 61  GIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAECVVKGLRRLGVERPCVL 120

Query: 121 VGFSYGAMVGFRLAQMYPDLVAAMVVTAAPTVLTASLSRGLLEKIGFESWSDYLIPATVK 180
           VGFSYGAMVGFRLA+MYPDLVAAMVVT+APTVLT SLSR  L++IG++SWSDYLIP TVK
Sbjct: 121 VGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTVLTESLSREALQEIGYKSWSDYLIPNTVK 180

Query: 181 GVRCMFEIGSFRARLALKLPAVPDWIFKHYLEV 212
           GV+ +FEIGSF        PA+P+WI + Y++V
Sbjct: 181 GVKSLFEIGSF------DFPALPNWIMEQYVKV 207

[Close]

BLAST of Lag0009171 vs. ExPASy TrEMBL
Match: A0A6J1G806 (epoxide hydrolase 4-like OS=Cucurbita moschata OX=3662 GN=LOC111451675 PE=4 SV=1)

HSP 1 Score: 331.3 bits (848), Expect = 3.0e-87
Identity = 159/212 (75.00%), Postives = 180/212 (84.91%), Query Frame = 0


Query: 1   MVNIFHLYDRVLRWLMKATGIRPEMVQIEPGTTMNFWVPTKN--NNPNPPVVLLHGFAAN 60
           M NIFH+Y RVLRWLMK  G+R E+VQIEPGTTMNFWVPT+N   N NPPVVLLHGFA N
Sbjct: 1   MANIFHVYHRVLRWLMKVAGVRQELVQIEPGTTMNFWVPTRNPKKNRNPPVVLLHGFAGN 60

Query: 61  AILTWQFQVLSLAKNHAVYVPDFIFFGDSVTDRPERSPEFQAECVAEGLRKLGVERPCVF 120
            I+TWQFQV++LA+N+AVYVPDF+FFG+SVTDR ERSPEFQAE V +GLRKLGVERPCV 
Sbjct: 61  GIMTWQFQVMNLARNYAVYVPDFLFFGESVTDRAERSPEFQAEGVVKGLRKLGVERPCVL 120

Query: 121 VGFSYGAMVGFRLAQMYPDLVAAMVVTAAPTVLTASLSRGLLEKIGFESWSDYLIPATVK 180
           VGFSYGAMVGFRLA+MYPDLVAAMVVT+APT LT SLSR  L +IG++SWSDYLIP TV 
Sbjct: 121 VGFSYGAMVGFRLAEMYPDLVAAMVVTSAPTALTESLSRVALLEIGYKSWSDYLIPNTVN 180

Query: 181 GVRCMFEIGSFRARLALKLPAVPDWIFKHYLE 211
           GV+ +FEIGSF        PA+P+WI KHYLE
Sbjct: 181 GVKSLFEIGSF------DFPALPNWIMKHYLE 206

[Close]

BLAST of Lag0009171 vs. ExPASy TrEMBL
Match: A0A5A7TQB4 (Epoxide hydrolase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold303G00590 PE=4 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 1.0e-82
Identity = 152/214 (71.03%), Postives = 174/214 (81.31%), Query Frame = 0


Query: 1   MVNIFHLYDRVLRWLMKATGIRPEMVQIEPGTTMNFWV----PTKNNNPNPPVVLLHGFA 60
           ++NIFH+YD VLRW+MKA GI P  +QIEPGT+MNFWV    P KN+  +PP++ LHGFA
Sbjct: 2   VINIFHIYDLVLRWVMKAAGIIPVQLQIEPGTSMNFWVPARKPNKNHQNHPPLLFLHGFA 61

Query: 61  ANAILTWQFQVLSLAKNHAVYVPDFIFFGDSVTDRPERSPEFQAECVAEGLRKLGVERPC 120
           ANAI+TWQFQVL  AK HAVYVPDF+FFGDSVTDRP+RSPEFQAECVAEGLRKLGVER  
Sbjct: 62  ANAIMTWQFQVLKFAKTHAVYVPDFMFFGDSVTDRPDRSPEFQAECVAEGLRKLGVERRF 121

Query: 121 VFVGFSYGAMVGFRLAQMYPDLVAAMVVTAAPTVLTASLSRGLLEKIGFESWSDYLIPAT 180
           V VGFSYGAMVGFRLA+ YP+LV AMVVTAAPTVLT  ++   LEKIG++SWS+YLIP T
Sbjct: 122 VLVGFSYGAMVGFRLAEKYPELVEAMVVTAAPTVLTERITEAALEKIGYKSWSEYLIPET 181

Query: 181 VKGVRCMFEIGSFRARLALKLPAVPDWIFKHYLE 211
           VKGV+ M EI SF      + P  P WIFKHYLE
Sbjct: 182 VKGVKSMLEIASF------EFPRFPRWIFKHYLE 209

[Close]

BLAST of Lag0009171 vs. ExPASy TrEMBL
Match: A0A1S4E310 (uncharacterized protein LOC103499739 OS=Cucumis melo OX=3656 GN=LOC103499739 PE=4 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 1.0e-82
Identity = 152/214 (71.03%), Postives = 174/214 (81.31%), Query Frame = 0


Query: 1   MVNIFHLYDRVLRWLMKATGIRPEMVQIEPGTTMNFWV----PTKNNNPNPPVVLLHGFA 60
           ++NIFH+YD VLRW+MKA GI P  +QIEPGT+MNFWV    P KN+  +PP++ LHGFA
Sbjct: 2   VINIFHIYDLVLRWVMKAAGIIPVQLQIEPGTSMNFWVPARKPNKNHQNHPPLLFLHGFA 61

Query: 61  ANAILTWQFQVLSLAKNHAVYVPDFIFFGDSVTDRPERSPEFQAECVAEGLRKLGVERPC 120
           ANAI+TWQFQVL  AK HAVYVPDF+FFGDSVTDRP+RSPEFQAECVAEGLRKLGVER  
Sbjct: 62  ANAIMTWQFQVLKFAKTHAVYVPDFMFFGDSVTDRPDRSPEFQAECVAEGLRKLGVERRF 121

Query: 121 VFVGFSYGAMVGFRLAQMYPDLVAAMVVTAAPTVLTASLSRGLLEKIGFESWSDYLIPAT 180
           V VGFSYGAMVGFRLA+ YP+LV AMVVTAAPTVLT  ++   LEKIG++SWS+YLIP T
Sbjct: 122 VLVGFSYGAMVGFRLAEKYPELVEAMVVTAAPTVLTERITEAALEKIGYKSWSEYLIPET 181

Query: 181 VKGVRCMFEIGSFRARLALKLPAVPDWIFKHYLE 211
           VKGV+ M EI SF      + P  P WIFKHYLE
Sbjct: 182 VKGVKSMLEIASF------EFPRFPRWIFKHYLE 209

[Close]

BLAST of Lag0009171 vs. ExPASy TrEMBL
Match: A0A0A0LLY6 (AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G247580 PE=4 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 3.0e-79
Identity = 145/214 (67.76%), Postives = 171/214 (79.91%), Query Frame = 0


Query: 1   MVNIFHLYDRVLRWLMKATGIRPEMVQIEPGTTMNFWV----PTKNNNPNPPVVLLHGFA 60
           MVNIFH+YD+VLRW+MKA GI+P  +QI+PGT MNFWV    P KN+  +PP++ LHGFA
Sbjct: 1   MVNIFHIYDQVLRWVMKAAGIKPVQLQIKPGTAMNFWVPAGKPNKNHQNHPPLLFLHGFA 60

Query: 61  ANAILTWQFQVLSLAKNHAVYVPDFIFFGDSVTDRPERSPEFQAECVAEGLRKLGVERPC 120
            NAI+TWQFQVL  AKNHAVYVPDF+FFGDSVTDRP+R+ EFQAECV EGLRKLGV+R  
Sbjct: 61  TNAIMTWQFQVLKFAKNHAVYVPDFMFFGDSVTDRPDRTTEFQAECVVEGLRKLGVDRRF 120

Query: 121 VFVGFSYGAMVGFRLAQMYPDLVAAMVVTAAPTVLTASLSRGLLEKIGFESWSDYLIPAT 180
           V VGFSYGAMVGFRLA+MYP++V AMVVTAAPTVLT  ++   +EKIG++SWS+YLIP T
Sbjct: 121 VLVGFSYGAMVGFRLAEMYPEMVEAMVVTAAPTVLTERITGEAMEKIGYKSWSEYLIPET 180

Query: 181 VKGVRCMFEIGSFRARLALKLPAVPDWIFKHYLE 211
           VKG   M +I SF      + P  P WIFK YLE
Sbjct: 181 VKGAISMLQIASF------EFPRFPRWIFKQYLE 208

[Close]

BLAST of Lag0009171 vs. TAIR 10
Match: AT4G36610.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 189.1 bits (479), Expect = 3.6e-48
Identity = 101/226 (44.69%), Postives = 143/226 (63.27%), Query Frame = 0


Query: 1   MVNIFHLYDRVLRWLMKATGIRPEMVQIEPGTTMNFWV-------------PTKNNNPNP 60
           MVN   +   +L  LMK  G+ P  ++IEPGT +NFWV             PTK + P  
Sbjct: 1   MVNFVEVQKPLLYGLMKMAGVVPYTLEIEPGTKINFWVPKETLKKNSGTGKPTKPDKPKK 60

Query: 61  PVVLL-HGFAANAILTWQFQVLSLAKNHAVYVPDFIFFGDSVTDRPERSPEFQAECVAEG 120
           PVVLL HGFA   I+TWQFQV +L+K ++VY+PD +FFG S TD  +RSP FQA+C+ +G
Sbjct: 61  PVVLLIHGFAGEGIVTWQFQVGALSKKYSVYIPDLLFFGGSYTDNSDRSPAFQADCLVKG 120

Query: 121 LRKLGVERPCVFVGFSYGAMVGFRLAQMYPDLVAAMVVTAAPTVLTASLSRGLLEKIGFE 180
           LR LGV++  V VGFSYG MV F++A+ YPD+V A+VV+ +   +T +++   L ++GF 
Sbjct: 121 LRILGVDK-FVPVGFSYGGMVAFKIAEAYPDMVRAIVVSGSIPTMTDTINEASLNRLGFS 180

Query: 181 SWSDYLIPATVKGVRCMFEIGSFRARLALKLPA-VPDWIFKHYLEV 212
           S +D L+P +V G++ +F I       A+  P   P  +FK Y+EV
Sbjct: 181 SSTDLLLPTSVTGLKALFTI-------AVHKPLWFPKRLFKDYIEV 218

[Close]

BLAST of Lag0009171 vs. TAIR 10
Match: AT2G18360.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 186.4 bits (472), Expect = 2.3e-47
Identity = 99/228 (43.42%), Postives = 143/228 (62.72%), Query Frame = 0


Query: 1   MVNIFHLYDRVLRWLMKATGIRPEMVQIEPGTTMNFWVP------TKNNNPN-------- 60
           MVN   L   +L  LMK  G+ P  V++EPGT MNFW+P      TK ++ N        
Sbjct: 1   MVNFVDLQKPLLYRLMKLAGVIPYTVELEPGTKMNFWIPKETLKKTKKSDKNFAVEPQKP 60

Query: 61  --PPVVLLHGFAANAILTWQFQVLSLAKNHAVYVPDFIFFGDSVTDRPERSPEFQAECVA 120
             P ++ +HGFAA  I+TWQFQV SLAK ++VY+PD +FFG S +D  +RSP FQA C+ 
Sbjct: 61  TKPVLLFIHGFAAEGIVTWQFQVGSLAKKYSVYIPDLLFFGGSYSDNADRSPAFQAHCLV 120

Query: 121 EGLRKLGVERPCVFVGFSYGAMVGFRLAQMYPDLVAAMVVTAAPTVLTASLSRGLLEKIG 180
           + LR LG+E+    VGFSYG MV F++A+ YP++V AMVV+ +   +T ++S   L ++G
Sbjct: 121 KSLRILGIEK-FTLVGFSYGGMVAFKIAEEYPEMVQAMVVSGSILAMTDTISESNLNQLG 180

Query: 181 FESWSDYLIPATVKGVRCMFEIGSFRARLALKLPA-VPDWIFKHYLEV 212
           F+S +D L+P +VKG++ +F        LA+  P   P  +FK ++EV
Sbjct: 181 FKSSADLLLPTSVKGLKTLF-------TLAVHKPMWFPKRLFKDFIEV 220

[Close]

BLAST of Lag0009171 vs. TAIR 10
Match: AT1G78210.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 117.9 bits (294), Expect = 1.0e-26
Identity = 68/193 (35.23%), Postives = 104/193 (53.89%), Query Frame = 0


Query: 9   DRVLRWLMKATGIRPEMVQIEPGTTMNFWV-PTK-NNNPNPPVVLLHGFAANAILTWQFQ 68
           +R  +   K +G+RP  + ++ GT +NFWV  TK  + P P ++L+HG  A AI  W   
Sbjct: 12  ERTYKSGFKRSGLRPVTIDLKDGTVVNFWVSKTKPESKPKPNLLLIHGLGATAIWQWYDV 71

Query: 69  VLSLAKNHAVYVPDFIFFGDSVTDRPERSPEFQAECVAEGLRKLGVERPCVFVGFSYGAM 128
              L++   +Y+PD +FFG S T RPERS  FQA+ +   L    V++    VG SYG  
Sbjct: 72  ARRLSRYFNLYIPDLVFFGGSSTTRPERSDIFQAQTLMRALEAQSVKK-FSLVGLSYGGF 131

Query: 129 VGFRLAQMYPDLVAAMVV-TAAPTVLTASLSRGLLEKIGFESWSDYLIPATVKGVRCMFE 188
           VG+R+A MY D V  +V+  AA  V    +  G+ +    +  S  L+P +VK +R +  
Sbjct: 132 VGYRMASMYADAVEKVVICCAAVCVEEKDMKAGVFKVSDLDEASKILVPESVKKLRELMG 191

Query: 189 IGSFRARLALKLP 199
              ++  LA  +P
Sbjct: 192 YIFYKPALARLVP 203

[Close]

BLAST of Lag0009171 vs. TAIR 10
Match: AT5G21950.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 109.0 bits (271), Expect = 4.7e-24
Identity = 62/213 (29.11%), Postives = 109/213 (51.17%), Query Frame = 0


Query: 2   VNIFHLYDRVLRWLMKATGIRPEMVQIEPGTTMNFWVP----TKNNNPNPPVVLLHGFAA 61
           +++    + +LR    + G+  + + I+  TT+ FW P    +  N   P ++LLHGF  
Sbjct: 6   LSVARFVEALLRRRFSSAGLSLQTLSIDSETTIQFWGPPPSSSSENTQKPSLLLLHGFGP 65

Query: 62  NAILTWQFQVLSLAKNHAVYVPDFIFFGDSVTDRPERSPEFQAECVAEGLRKLGVERPCV 121
           +A+  W  QV  L+    +YVPD +FFG S +    RS  FQA C+ + + KL VER  V
Sbjct: 66  SAVWQWSHQVKPLSHFFRLYVPDLVFFGGSSSSGENRSEMFQALCMGKLMEKLEVERFSV 125

Query: 122 FVGFSYGAMVGFRLAQMYPDLVAAMVVTAAPTVLTASLSRGLLEKIGFESWSDYLIPATV 181
            VG SYG  V + +A+M+P+ V  +V+ ++   L  S +   + +       + ++PA+ 
Sbjct: 126 -VGTSYGGFVAYNMAKMFPEKVEKVVLASSGVNLRRSDNEAFIARAKCHRIKEVMLPASA 185

Query: 182 KGVRCMFEIGSFRARLALKLPAVPDWIFKHYLE 211
             +R    + S +     +L  VPD++   + +
Sbjct: 186 TDLRRFSGMVSSK-----RLDYVPDFVLNDFCQ 212

[Close]

BLAST of Lag0009171 vs. TAIR 10
Match: AT4G39955.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 102.4 bits (254), Expect = 4.4e-22
Identity = 61/206 (29.61%), Postives = 97/206 (47.09%), Query Frame = 0


Query: 9   DRVLRWLMKATGIRPEMVQIEPGTTMNFWVPTKNNNPNPPVVLLHGFAANAILTWQFQVL 68
           ++  ++     G+R     +  GT  + W+P  + +  P ++LLHG  ANA+  W   + 
Sbjct: 12  NKCYQYSFSRAGLRSSTSDLGDGTVFHCWIPLTHIHTKPTLLLLHGIGANAMWQWDRFID 71

Query: 69  SLAKNHAVYVPDFIFFGDSVTDRPERSPEFQAECVAEGLRKLGVERPCVFVGFSYGAMVG 128
                  VYVPD IFFGDS T RP+RS  FQA CV + +   GV R     G SYG  V 
Sbjct: 72  RFIPRFNVYVPDLIFFGDSYTTRPDRSESFQATCVMKAMDAYGV-RTMTVAGLSYGGFVA 131

Query: 129 FRLAQMYPDLVAAMVVTAAPTVLTASLSR-GLLEKIGFESWSDYLIPATVKGVRCMFEIG 188
           + LA  + + V  +V+  A   L    S  G+ +    E  +  L P +   +R + ++ 
Sbjct: 132 YSLAAQFKERVDRVVLICAGVALEEKDSEDGMFKVKSPEEAAAVLFPQSPSMLRRLLQLS 191

Query: 189 SFRARLALKLPAVPDWIF---KHYLE 211
            ++  + +      D+I    K YL+
Sbjct: 192 FYKPPIWIPSCFAMDYIHVMCKDYLQ 216

The following BLAST results are available for this feature:

BLAST of Lag0009171 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6605077.1	1.4e-89	75.47	Epoxide hydrolase 3, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023533135.1	1.4e-89	75.47	epoxide hydrolase 4-like [Cucurbita pepo subsp. pepo]	[more]
KAG7035085.1	3.9e-89	75.00	Epoxide hydrolase 4, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023006837.1	1.5e-88	74.18	epoxide hydrolase 4-like [Cucurbita maxima]	[more]
XP_022947943.1	6.3e-87	75.00	epoxide hydrolase 4-like [Cucurbita moschata]	[more]

BLAST of Lag0009171 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9A919	8.2e-05	24.83	Haloalkane dehalogenase OS=Caulobacter vibrioides (strain ATCC 19089 / CB15) OX=...	[more]
B8H3S9	8.2e-05	24.83	Haloalkane dehalogenase OS=Caulobacter vibrioides (strain NA1000 / CB15N) OX=565...	[more]
P34913	1.4e-04	26.70	Bifunctional epoxide hydrolase 2 OS=Homo sapiens OX=9606 GN=EPHX2 PE=1 SV=2	[more]
B0SY51	1.8e-04	29.32	Haloalkane dehalogenase OS=Caulobacter sp. (strain K31) OX=366602 GN=dhmA PE=3 S...	[more]

BLAST of Lag0009171 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KWX2	7.2e-89	74.18	epoxide hydrolase 4-like OS=Cucurbita maxima OX=3661 GN=LOC111499509 PE=4 SV=1	[more]
A0A6J1G806	3.0e-87	75.00	epoxide hydrolase 4-like OS=Cucurbita moschata OX=3662 GN=LOC111451675 PE=4 SV=1	[more]
A0A5A7TQB4	1.0e-82	71.03	Epoxide hydrolase 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffol...	[more]
A0A1S4E310	1.0e-82	71.03	uncharacterized protein LOC103499739 OS=Cucumis melo OX=3656 GN=LOC103499739 PE=...	[more]
A0A0A0LLY6	3.0e-79	67.76	AB hydrolase-1 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G247...	[more]

BLAST of Lag0009171 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G36610.1	3.6e-48	44.69	alpha/beta-Hydrolases superfamily protein	[more]
AT2G18360.1	2.3e-47	43.42	alpha/beta-Hydrolases superfamily protein	[more]
AT1G78210.1	1.0e-26	35.23	alpha/beta-Hydrolases superfamily protein	[more]
AT5G21950.1	4.7e-24	29.11	alpha/beta-Hydrolases superfamily protein	[more]
AT4G39955.1	4.4e-22	29.61	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000073	Alpha/beta hydrolase fold-1	PFAM	PF00561	Abhydrolase_1	coord: 47..149 e-value: 5.1E-14 score: 52.5
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 6..210 e-value: 1.9E-28 score: 101.9
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 20..180
None	No IPR available	PANTHER	PTHR43139:SF22	EPOXIDE HYDROLASE 4-LIKE	coord: 2..211
None	No IPR available	PANTHER	PTHR43139	SI:DKEY-122A22.2	coord: 2..211

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0009171.1	Lag0009171.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016787	hydrolase activity