Lag0009159 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0009159
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Retrovirus-related Pol polyprotein from transposon TNT 1-94
Location	chr9: 36174096 .. 36174611 (-)
RNA-Seq Expression	Lag0009159
Synteny	Lag0009159

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATGCCTCGAAAGATCTGGAACAGAATTTAGATGAGTATAATCGAATTACTCTAGATCTAAATAATATAGGTAAGGCTGTATCTGATGAGAATAAGGTTATAATACTGTTAAATTCCCTTCCAAATTCTTATAATGAGGTGAAAACCACAATCAAATATGGTAGAGATAGTTTAACTATGGAAATTGTACTGAATGCTCTTAGATCCAGAGACCTAGAAATGAAAAAGGCAAAGGAAAAAATAATGAGGCTTTGGATAGCAAGAGGAAGAACTGAAAAGAAATCCTCTAAAGGTACTAGTAGCAGTCGGGGTAGATCTAAGTCCCAAGGGAAAACCAAGAGGTGTTATTATTGTCAAAAGGAGGGGCACATTAGAAGAAACTGTTATGAATTAAAGAATAAGAGGAAGAATGAAGGTAAAAAGGAAGAGGAACCTCACAATGTGAATGTTTCAGAGGGTTATGATTTTGCTGTAGTGCTGATGGTTTCTGAAATTGAAATTAATAGTGATTGA

mRNA sequence

ATGGATGCCTCGAAAGATCTGGAACAGAATTTAGATGAGTATAATCGAATTACTCTAGATCTAAATAATATAGGTAAGGCTGTATCTGATGAGAATAAGGTTATAATACTGTTAAATTCCCTTCCAAATTCTTATAATGAGGTGAAAACCACAATCAAATATGGTAGAGATAGTTTAACTATGGAAATTGTACTGAATGCTCTTAGATCCAGAGACCTAGAAATGAAAAAGGCAAAGGAAAAAATAATGAGGCTTTGGATAGCAAGAGGAAGAACTGAAAAGAAATCCTCTAAAGGTACTAGTAGCAGTCGGGGTAGATCTAAGTCCCAAGGGAAAACCAAGAGGTGTTATTATTGTCAAAAGGAGGGGCACATTAGAAGAAACTGTTATGAATTAAAGAATAAGAGGAAGAATGAAGGTAAAAAGGAAGAGGAACCTCACAATGTGAATGTTTCAGAGGGTTATGATTTTGCTGTAGTGCTGATGGTTTCTGAAATTGAAATTAATAGTGATTGA

Coding sequence (CDS)

ATGGATGCCTCGAAAGATCTGGAACAGAATTTAGATGAGTATAATCGAATTACTCTAGATCTAAATAATATAGGTAAGGCTGTATCTGATGAGAATAAGGTTATAATACTGTTAAATTCCCTTCCAAATTCTTATAATGAGGTGAAAACCACAATCAAATATGGTAGAGATAGTTTAACTATGGAAATTGTACTGAATGCTCTTAGATCCAGAGACCTAGAAATGAAAAAGGCAAAGGAAAAAATAATGAGGCTTTGGATAGCAAGAGGAAGAACTGAAAAGAAATCCTCTAAAGGTACTAGTAGCAGTCGGGGTAGATCTAAGTCCCAAGGGAAAACCAAGAGGTGTTATTATTGTCAAAAGGAGGGGCACATTAGAAGAAACTGTTATGAATTAAAGAATAAGAGGAAGAATGAAGGTAAAAAGGAAGAGGAACCTCACAATGTGAATGTTTCAGAGGGTTATGATTTTGCTGTAGTGCTGATGGTTTCTGAAATTGAAATTAATAGTGATTGA

Protein sequence

MDASKDLEQNLDEYNRITLDLNNIGKAVSDENKVIILLNSLPNSYNEVKTTIKYGRDSLTMEIVLNALRSRDLEMKKAKEKIMRLWIARGRTEKKSSKGTSSSRGRSKSQGKTKRCYYCQKEGHIRRNCYELKNKRKNEGKKEEEPHNVNVSEGYDFAVVLMVSEIEINSD

Homology

[Close]

BLAST of Lag0009159 vs. NCBI nr
Match: TYK25306.1 (putative gag-pol polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 150.2 bits (378), Expect = 1.6e-32
Identity = 91/177 (51.41%), Postives = 125/177 (70.62%), Query Frame = 0


Query: 1   MDASKDLEQNLDEYNRITLDLNNIGKAVSDENKVIILLNSLPNSYNEVKTTIKYGRDSLT 60
           MD SK LE+NLDE+ +I +DLNNIG+ +SDEN+ +ILLNSLP +Y EVK  IKYGRDSLT
Sbjct: 111 MDQSKSLEENLDEFQKIVVDLNNIGEKMSDENQAVILLNSLPETYREVKAAIKYGRDSLT 170

Query: 61  MEIVLNALRSRDLEMKKAKEKIMRLWIARGRTEKKSSKGTSSSRGRSKSQGKTKRCYYCQ 120
           M IVL+AL++R+LE+KK + K   L +ARGR+EKKS KG   S  RSKS+GK+++C+ C 
Sbjct: 171 MSIVLDALKTRNLEIKK-ERKDGELLMARGRSEKKSWKGKERS-FRSKSKGKSRKCFLCH 230

Query: 121 KEGHIRRNCYELKNKR--KNEGKKEEEPHNVNVSEGYD-------FAVVLMVSEIEI 169
           KEGH ++NC   K++    +E    +  ++  +++GYD        A VLMVS  +I
Sbjct: 231 KEGHFKKNCPLNKSREASTSEANVTDGYNSAEITDGYDSAETGYESAEVLMVSHRDI 285

[Close]

BLAST of Lag0009159 vs. NCBI nr
Match: KAA0050719.1 (putative gag-pol polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 150.2 bits (378), Expect = 1.6e-32
Identity = 91/177 (51.41%), Postives = 125/177 (70.62%), Query Frame = 0


Query: 1   MDASKDLEQNLDEYNRITLDLNNIGKAVSDENKVIILLNSLPNSYNEVKTTIKYGRDSLT 60
           MD SK LE+NLDE+ +I +DLNNIG+ +SDEN+ +ILLNSLP +Y EVK  IKYGRDSLT
Sbjct: 111 MDQSKSLEENLDEFQKIVVDLNNIGEKMSDENQAVILLNSLPETYREVKAAIKYGRDSLT 170

Query: 61  MEIVLNALRSRDLEMKKAKEKIMRLWIARGRTEKKSSKGTSSSRGRSKSQGKTKRCYYCQ 120
           M IVL+AL++R+LE+KK + K   L +ARGR+EKKS KG   S  RSKS+GK+++C+ C 
Sbjct: 171 MSIVLDALKTRNLEIKK-ERKDGELLMARGRSEKKSWKGKERS-FRSKSKGKSRKCFLCH 230

Query: 121 KEGHIRRNCYELKNKR--KNEGKKEEEPHNVNVSEGYD-------FAVVLMVSEIEI 169
           KEGH ++NC   K++    +E    +  ++  +++GYD        A VLMVS  +I
Sbjct: 231 KEGHFKKNCPLNKSREASTSEANVTDGYNSAEITDGYDSAETGYESAEVLMVSHRDI 285

[Close]

BLAST of Lag0009159 vs. NCBI nr
Match: KAA0043826.1 (putative gag-pol polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 149.4 bits (376), Expect = 2.7e-32
Identity = 92/178 (51.69%), Postives = 121/178 (67.98%), Query Frame = 0


Query: 1   MDASKDLEQNLDEYNRITLDLNNIGKAVSDENKVIILLNSLPNSYNEVKTTIKYGRDSLT 60
           MD SK LE+NLDE+ +I +DLNNIG+ +SDEN+ +ILLNSLP +Y EVK  IKYGRDSLT
Sbjct: 1   MDQSKSLEENLDEFQKIVVDLNNIGEKMSDENQAVILLNSLPETYREVKAAIKYGRDSLT 60

Query: 61  MEIVLNALRSRDLEMKKAKEKIMRLWIARGRTEKKSSKGTSSSRGRSKSQGKTKRCYYCQ 120
           M IVL+AL++R+LE+KK + K   L +ARGR+EKKS KG   S  RSKS+GK+++C+ C 
Sbjct: 61  MSIVLDALKTRNLEIKK-ERKDGELLMARGRSEKKSWKGKERS-FRSKSKGKSRKCFLCH 120

Query: 121 KEGHIRRNCYELKNKRKNEGKKEEEPHNVNVSE----------GYDFAVVLMVSEIEI 169
           KEGH ++NC  L   R+    +       N +E          GY+ A VLMVS  +I
Sbjct: 121 KEGHFKKNC-PLNKSREASTSEANVTDGYNSAEITNGYDSAETGYESAEVLMVSHRDI 175

[Close]

BLAST of Lag0009159 vs. NCBI nr
Match: KAA0067607.1 (retrotransposon protein, putative, Ty1-copia subclass [Cucumis melo var. makuwa] >TYJ97155.1 retrotransposon protein, putative, Ty1-copia subclass [Cucumis melo var. makuwa])

HSP 1 Score: 146.4 bits (368), Expect = 2.3e-31
Identity = 88/170 (51.76%), Postives = 123/170 (72.35%), Query Frame = 0


Query: 1   MDASKDLEQNLDEYNRITLDLNNIGKAVSDENKVIILLNSLPNSYNEVKTTIKYGRDSLT 60
           MD SK LE+NLDE+ +I +DL+NIG+ +SDEN+ +ILLNSLP +Y EVK  IKYGRDSLT
Sbjct: 107 MDQSKSLEENLDEFQKIIVDLSNIGEKMSDENQAVILLNSLPETYREVKAAIKYGRDSLT 166

Query: 61  MEIVLNALRSRDLEMKKAKEKIMRLWIARGRTEKKSSKGTSSSRGRSKSQGKTKRCYYCQ 120
           M IVL+AL++R+LE+KK + K   L +ARGR+EKKS KG   S  RSKS+GK+++C+ C 
Sbjct: 167 MSIVLDALKTRNLEIKK-ERKDGELLMARGRSEKKSWKGKERS-SRSKSKGKSRKCFLCH 226

Query: 121 KEGHIRRNCYELKNKR--KNEGKKEEEPHNVNVSEGYDFAVVLMVSEIEI 169
           K GH ++NC   K++    +E    +  +   +++GY+ A VLMVS  +I
Sbjct: 227 K-GHFKKNCPLNKSREASTSEANVTDGYNFAEITDGYESAEVLMVSHRDI 273

[Close]

BLAST of Lag0009159 vs. NCBI nr
Match: KAA0047995.1 (retrotransposon protein, putative, Ty1-copia sub-class [Cucumis melo var. makuwa])

HSP 1 Score: 145.2 bits (365), Expect = 5.2e-31
Identity = 91/178 (51.12%), Postives = 120/178 (67.42%), Query Frame = 0


Query: 1   MDASKDLEQNLDEYNRITLDLNNIGKAVSDENKVIILLNSLPNSYNEVKTTIKYGRDSLT 60
           MD SK LE+NLDE+ +I +DLNNIG+ +SDEN+ +ILLNSLP +Y EVK  IKYG DSLT
Sbjct: 111 MDQSKILEENLDEFQKIVVDLNNIGEKMSDENQAVILLNSLPETYREVKAAIKYGWDSLT 170

Query: 61  MEIVLNALRSRDLEMKKAKEKIMRLWIARGRTEKKSSKGTSSSRGRSKSQGKTKRCYYCQ 120
           M IVL+AL++R+LE+KK + K   L +ARGR+EKKS KG   S  RSKS+GK+++C+ C 
Sbjct: 171 MSIVLDALKTRNLEIKK-ERKDGELLMARGRSEKKSWKGKERS-FRSKSKGKSRKCFLCH 230

Query: 121 KEGHIRRNCYELKNKRKNEGKKEEEPHNVNVSE----------GYDFAVVLMVSEIEI 169
           KEGH ++NC  L   R+    +       N +E          GY+ A VLMVS  +I
Sbjct: 231 KEGHFKKNC-PLNKSREASTSEANVTDGYNSAEITDGCDSAETGYESAEVLMVSHRDI 285

[Close]

BLAST of Lag0009159 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 1.9e-12
Identity = 56/174 (32.18%), Postives = 100/174 (57.47%), Query Frame = 0


Query: 1   MDASKDLEQNLDEYNRITLDLNNIGKAVSDENKVIILLNSLPNSYNEVKTTIKYGRDSLT 60
           M    +   +L+ +N +   L N+G  + +E+K I+LLNSLP+SY+ + TTI +G+ ++ 
Sbjct: 112 MSEGTNFLSHLNVFNGLITQLANLGVKIEEEDKAILLLNSLPSSYDNLATTILHGKTTIE 171

Query: 61  MEIVLNALRSRDLEMKKAKEKIMRLWI-ARGRTEKKSSK--GTSSSRGRSKSQGKT--KR 120
           ++ V +AL   +   KK + +   L    RGR+ ++SS   G S +RG+SK++ K+  + 
Sbjct: 172 LKDVTSALLLNEKMRKKPENQGQALITEGRGRSYQRSSNNYGRSGARGKSKNRSKSRVRN 231

Query: 121 CYYCQKEGHIRRNCYELKNKRKNEGKKEEEPHNVNVSE--GYDFAVVLMVSEIE 168
           CY C + GH +R+C    N RK +G+   + ++ N +     +  VVL ++E E
Sbjct: 232 CYNCNQPGHFKRDC---PNPRKGKGETSGQKNDDNTAAMVQNNDNVVLFINEEE 282

[Close]

BLAST of Lag0009159 vs. ExPASy Swiss-Prot
Match: P04146 (Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3)

HSP 1 Score: 45.4 bits (106), Expect = 7.3e-04
Identity = 39/158 (24.68%), Postives = 77/158 (48.73%), Query Frame = 0


Query: 14  YNRITLDLNNIGKAVSDENKVIILLNSLPNSYNEVKTTIK-YGRDSLTMEIVLNALRSRD 73
           ++ +  +L   G  + + +K+  LL +LP+ Y+ + T I+    ++LT+  V N L  ++
Sbjct: 121 FDELISELLAAGAKIEEMDKISHLLITLPSCYDGIITAIETLSEENLTLAFVKNRLLDQE 180

Query: 74  LEMKK----AKEKIMRLWIARGRTEKKS-------SKGTSSSRGRSKSQGKTKRCYYCQK 133
           +++K       +K+M   +       K+       +K     +G SK + K   C++C +
Sbjct: 181 IKIKNDHNDTSKKVMNAIVHNNNNTYKNNLFKNRVTKPKKIFKGNSKYKVK---CHHCGR 240

Query: 134 EGHIRRNCYELKNKRKNEGKKEEEPHNVNVSEGYDFAV 160
           EGHI+++C+  K    N+ K+ E+      S G  F V
Sbjct: 241 EGHIKKDCFHYKRILNNKNKENEKQVQTATSHGIAFMV 275

The following BLAST results are available for this feature:

BLAST of Lag0009159 vs. NCBI nr


Match Name	E-value	Identity	Description
TYK25306.1	1.6e-32	51.41	putative gag-pol polyprotein [Cucumis melo var. makuwa]	[more]
KAA0050719.1	1.6e-32	51.41	putative gag-pol polyprotein [Cucumis melo var. makuwa]	[more]
KAA0043826.1	2.7e-32	51.69	putative gag-pol polyprotein [Cucumis melo var. makuwa]	[more]
KAA0067607.1	2.3e-31	51.76	retrotransposon protein, putative, Ty1-copia subclass [Cucumis melo var. makuwa]...	[more]
KAA0047995.1	5.2e-31	51.12	retrotransposon protein, putative, Ty1-copia sub-class [Cucumis melo var. makuwa...	[more]

BLAST of Lag0009159 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P10978	1.9e-12	32.18	Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum...	[more]
P04146	7.3e-04	24.68	Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001878	Zinc finger, CCHC-type	SMART	SM00343	c2hcfinal6	coord: 115..131 e-value: 0.003 score: 26.7
IPR001878	Zinc finger, CCHC-type	PROSITE	PS50158	ZF_CCHC	coord: 115..129 score: 9.504374
None	No IPR available	GENE3D	4.10.60.10		coord: 87..146 e-value: 6.4E-7 score: 31.1
None	No IPR available	PFAM	PF14223	Retrotran_gag_2	coord: 1..76 e-value: 2.4E-12 score: 46.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 92..113
IPR036875	Zinc finger, CCHC-type superfamily	SUPERFAMILY	57756	Retrovirus zinc finger-like domains	coord: 98..137

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0009159.1	Lag0009159.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0008270	zinc ion binding