Lag0009159 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0009159
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRetrovirus-related Pol polyprotein from transposon TNT 1-94
Locationchr9: 36174096 .. 36174611 (-)
RNA-Seq ExpressionLag0009159
SyntenyLag0009159
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGCCTCGAAAGATCTGGAACAGAATTTAGATGAGTATAATCGAATTACTCTAGATCTAAATAATATAGGTAAGGCTGTATCTGATGAGAATAAGGTTATAATACTGTTAAATTCCCTTCCAAATTCTTATAATGAGGTGAAAACCACAATCAAATATGGTAGAGATAGTTTAACTATGGAAATTGTACTGAATGCTCTTAGATCCAGAGACCTAGAAATGAAAAAGGCAAAGGAAAAAATAATGAGGCTTTGGATAGCAAGAGGAAGAACTGAAAAGAAATCCTCTAAAGGTACTAGTAGCAGTCGGGGTAGATCTAAGTCCCAAGGGAAAACCAAGAGGTGTTATTATTGTCAAAAGGAGGGGCACATTAGAAGAAACTGTTATGAATTAAAGAATAAGAGGAAGAATGAAGGTAAAAAGGAAGAGGAACCTCACAATGTGAATGTTTCAGAGGGTTATGATTTTGCTGTAGTGCTGATGGTTTCTGAAATTGAAATTAATAGTGATTGA

mRNA sequence

ATGGATGCCTCGAAAGATCTGGAACAGAATTTAGATGAGTATAATCGAATTACTCTAGATCTAAATAATATAGGTAAGGCTGTATCTGATGAGAATAAGGTTATAATACTGTTAAATTCCCTTCCAAATTCTTATAATGAGGTGAAAACCACAATCAAATATGGTAGAGATAGTTTAACTATGGAAATTGTACTGAATGCTCTTAGATCCAGAGACCTAGAAATGAAAAAGGCAAAGGAAAAAATAATGAGGCTTTGGATAGCAAGAGGAAGAACTGAAAAGAAATCCTCTAAAGGTACTAGTAGCAGTCGGGGTAGATCTAAGTCCCAAGGGAAAACCAAGAGGTGTTATTATTGTCAAAAGGAGGGGCACATTAGAAGAAACTGTTATGAATTAAAGAATAAGAGGAAGAATGAAGGTAAAAAGGAAGAGGAACCTCACAATGTGAATGTTTCAGAGGGTTATGATTTTGCTGTAGTGCTGATGGTTTCTGAAATTGAAATTAATAGTGATTGA

Coding sequence (CDS)

ATGGATGCCTCGAAAGATCTGGAACAGAATTTAGATGAGTATAATCGAATTACTCTAGATCTAAATAATATAGGTAAGGCTGTATCTGATGAGAATAAGGTTATAATACTGTTAAATTCCCTTCCAAATTCTTATAATGAGGTGAAAACCACAATCAAATATGGTAGAGATAGTTTAACTATGGAAATTGTACTGAATGCTCTTAGATCCAGAGACCTAGAAATGAAAAAGGCAAAGGAAAAAATAATGAGGCTTTGGATAGCAAGAGGAAGAACTGAAAAGAAATCCTCTAAAGGTACTAGTAGCAGTCGGGGTAGATCTAAGTCCCAAGGGAAAACCAAGAGGTGTTATTATTGTCAAAAGGAGGGGCACATTAGAAGAAACTGTTATGAATTAAAGAATAAGAGGAAGAATGAAGGTAAAAAGGAAGAGGAACCTCACAATGTGAATGTTTCAGAGGGTTATGATTTTGCTGTAGTGCTGATGGTTTCTGAAATTGAAATTAATAGTGATTGA

Protein sequence

MDASKDLEQNLDEYNRITLDLNNIGKAVSDENKVIILLNSLPNSYNEVKTTIKYGRDSLTMEIVLNALRSRDLEMKKAKEKIMRLWIARGRTEKKSSKGTSSSRGRSKSQGKTKRCYYCQKEGHIRRNCYELKNKRKNEGKKEEEPHNVNVSEGYDFAVVLMVSEIEINSD
Homology
BLAST of Lag0009159 vs. NCBI nr
Match: TYK25306.1 (putative gag-pol polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 150.2 bits (378), Expect = 1.6e-32
Identity = 91/177 (51.41%), Postives = 125/177 (70.62%), Query Frame = 0

Query: 1   MDASKDLEQNLDEYNRITLDLNNIGKAVSDENKVIILLNSLPNSYNEVKTTIKYGRDSLT 60
           MD SK LE+NLDE+ +I +DLNNIG+ +SDEN+ +ILLNSLP +Y EVK  IKYGRDSLT
Sbjct: 111 MDQSKSLEENLDEFQKIVVDLNNIGEKMSDENQAVILLNSLPETYREVKAAIKYGRDSLT 170

Query: 61  MEIVLNALRSRDLEMKKAKEKIMRLWIARGRTEKKSSKGTSSSRGRSKSQGKTKRCYYCQ 120
           M IVL+AL++R+LE+KK + K   L +ARGR+EKKS KG   S  RSKS+GK+++C+ C 
Sbjct: 171 MSIVLDALKTRNLEIKK-ERKDGELLMARGRSEKKSWKGKERS-FRSKSKGKSRKCFLCH 230

Query: 121 KEGHIRRNCYELKNKR--KNEGKKEEEPHNVNVSEGYD-------FAVVLMVSEIEI 169
           KEGH ++NC   K++    +E    +  ++  +++GYD        A VLMVS  +I
Sbjct: 231 KEGHFKKNCPLNKSREASTSEANVTDGYNSAEITDGYDSAETGYESAEVLMVSHRDI 285

BLAST of Lag0009159 vs. NCBI nr
Match: KAA0050719.1 (putative gag-pol polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 150.2 bits (378), Expect = 1.6e-32
Identity = 91/177 (51.41%), Postives = 125/177 (70.62%), Query Frame = 0

Query: 1   MDASKDLEQNLDEYNRITLDLNNIGKAVSDENKVIILLNSLPNSYNEVKTTIKYGRDSLT 60
           MD SK LE+NLDE+ +I +DLNNIG+ +SDEN+ +ILLNSLP +Y EVK  IKYGRDSLT
Sbjct: 111 MDQSKSLEENLDEFQKIVVDLNNIGEKMSDENQAVILLNSLPETYREVKAAIKYGRDSLT 170

Query: 61  MEIVLNALRSRDLEMKKAKEKIMRLWIARGRTEKKSSKGTSSSRGRSKSQGKTKRCYYCQ 120
           M IVL+AL++R+LE+KK + K   L +ARGR+EKKS KG   S  RSKS+GK+++C+ C 
Sbjct: 171 MSIVLDALKTRNLEIKK-ERKDGELLMARGRSEKKSWKGKERS-FRSKSKGKSRKCFLCH 230

Query: 121 KEGHIRRNCYELKNKR--KNEGKKEEEPHNVNVSEGYD-------FAVVLMVSEIEI 169
           KEGH ++NC   K++    +E    +  ++  +++GYD        A VLMVS  +I
Sbjct: 231 KEGHFKKNCPLNKSREASTSEANVTDGYNSAEITDGYDSAETGYESAEVLMVSHRDI 285

BLAST of Lag0009159 vs. NCBI nr
Match: KAA0043826.1 (putative gag-pol polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 149.4 bits (376), Expect = 2.7e-32
Identity = 92/178 (51.69%), Postives = 121/178 (67.98%), Query Frame = 0

Query: 1   MDASKDLEQNLDEYNRITLDLNNIGKAVSDENKVIILLNSLPNSYNEVKTTIKYGRDSLT 60
           MD SK LE+NLDE+ +I +DLNNIG+ +SDEN+ +ILLNSLP +Y EVK  IKYGRDSLT
Sbjct: 1   MDQSKSLEENLDEFQKIVVDLNNIGEKMSDENQAVILLNSLPETYREVKAAIKYGRDSLT 60

Query: 61  MEIVLNALRSRDLEMKKAKEKIMRLWIARGRTEKKSSKGTSSSRGRSKSQGKTKRCYYCQ 120
           M IVL+AL++R+LE+KK + K   L +ARGR+EKKS KG   S  RSKS+GK+++C+ C 
Sbjct: 61  MSIVLDALKTRNLEIKK-ERKDGELLMARGRSEKKSWKGKERS-FRSKSKGKSRKCFLCH 120

Query: 121 KEGHIRRNCYELKNKRKNEGKKEEEPHNVNVSE----------GYDFAVVLMVSEIEI 169
           KEGH ++NC  L   R+    +       N +E          GY+ A VLMVS  +I
Sbjct: 121 KEGHFKKNC-PLNKSREASTSEANVTDGYNSAEITNGYDSAETGYESAEVLMVSHRDI 175

BLAST of Lag0009159 vs. NCBI nr
Match: KAA0067607.1 (retrotransposon protein, putative, Ty1-copia subclass [Cucumis melo var. makuwa] >TYJ97155.1 retrotransposon protein, putative, Ty1-copia subclass [Cucumis melo var. makuwa])

HSP 1 Score: 146.4 bits (368), Expect = 2.3e-31
Identity = 88/170 (51.76%), Postives = 123/170 (72.35%), Query Frame = 0

Query: 1   MDASKDLEQNLDEYNRITLDLNNIGKAVSDENKVIILLNSLPNSYNEVKTTIKYGRDSLT 60
           MD SK LE+NLDE+ +I +DL+NIG+ +SDEN+ +ILLNSLP +Y EVK  IKYGRDSLT
Sbjct: 107 MDQSKSLEENLDEFQKIIVDLSNIGEKMSDENQAVILLNSLPETYREVKAAIKYGRDSLT 166

Query: 61  MEIVLNALRSRDLEMKKAKEKIMRLWIARGRTEKKSSKGTSSSRGRSKSQGKTKRCYYCQ 120
           M IVL+AL++R+LE+KK + K   L +ARGR+EKKS KG   S  RSKS+GK+++C+ C 
Sbjct: 167 MSIVLDALKTRNLEIKK-ERKDGELLMARGRSEKKSWKGKERS-SRSKSKGKSRKCFLCH 226

Query: 121 KEGHIRRNCYELKNKR--KNEGKKEEEPHNVNVSEGYDFAVVLMVSEIEI 169
           K GH ++NC   K++    +E    +  +   +++GY+ A VLMVS  +I
Sbjct: 227 K-GHFKKNCPLNKSREASTSEANVTDGYNFAEITDGYESAEVLMVSHRDI 273

BLAST of Lag0009159 vs. NCBI nr
Match: KAA0047995.1 (retrotransposon protein, putative, Ty1-copia sub-class [Cucumis melo var. makuwa])

HSP 1 Score: 145.2 bits (365), Expect = 5.2e-31
Identity = 91/178 (51.12%), Postives = 120/178 (67.42%), Query Frame = 0

Query: 1   MDASKDLEQNLDEYNRITLDLNNIGKAVSDENKVIILLNSLPNSYNEVKTTIKYGRDSLT 60
           MD SK LE+NLDE+ +I +DLNNIG+ +SDEN+ +ILLNSLP +Y EVK  IKYG DSLT
Sbjct: 111 MDQSKILEENLDEFQKIVVDLNNIGEKMSDENQAVILLNSLPETYREVKAAIKYGWDSLT 170

Query: 61  MEIVLNALRSRDLEMKKAKEKIMRLWIARGRTEKKSSKGTSSSRGRSKSQGKTKRCYYCQ 120
           M IVL+AL++R+LE+KK + K   L +ARGR+EKKS KG   S  RSKS+GK+++C+ C 
Sbjct: 171 MSIVLDALKTRNLEIKK-ERKDGELLMARGRSEKKSWKGKERS-FRSKSKGKSRKCFLCH 230

Query: 121 KEGHIRRNCYELKNKRKNEGKKEEEPHNVNVSE----------GYDFAVVLMVSEIEI 169
           KEGH ++NC  L   R+    +       N +E          GY+ A VLMVS  +I
Sbjct: 231 KEGHFKKNC-PLNKSREASTSEANVTDGYNSAEITDGCDSAETGYESAEVLMVSHRDI 285

BLAST of Lag0009159 vs. ExPASy Swiss-Prot
Match: P10978 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum OX=4097 PE=2 SV=1)

HSP 1 Score: 73.9 bits (180), Expect = 1.9e-12
Identity = 56/174 (32.18%), Postives = 100/174 (57.47%), Query Frame = 0

Query: 1   MDASKDLEQNLDEYNRITLDLNNIGKAVSDENKVIILLNSLPNSYNEVKTTIKYGRDSLT 60
           M    +   +L+ +N +   L N+G  + +E+K I+LLNSLP+SY+ + TTI +G+ ++ 
Sbjct: 112 MSEGTNFLSHLNVFNGLITQLANLGVKIEEEDKAILLLNSLPSSYDNLATTILHGKTTIE 171

Query: 61  MEIVLNALRSRDLEMKKAKEKIMRLWI-ARGRTEKKSSK--GTSSSRGRSKSQGKT--KR 120
           ++ V +AL   +   KK + +   L    RGR+ ++SS   G S +RG+SK++ K+  + 
Sbjct: 172 LKDVTSALLLNEKMRKKPENQGQALITEGRGRSYQRSSNNYGRSGARGKSKNRSKSRVRN 231

Query: 121 CYYCQKEGHIRRNCYELKNKRKNEGKKEEEPHNVNVSE--GYDFAVVLMVSEIE 168
           CY C + GH +R+C    N RK +G+   + ++ N +     +  VVL ++E E
Sbjct: 232 CYNCNQPGHFKRDC---PNPRKGKGETSGQKNDDNTAAMVQNNDNVVLFINEEE 282

BLAST of Lag0009159 vs. ExPASy Swiss-Prot
Match: P04146 (Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3)

HSP 1 Score: 45.4 bits (106), Expect = 7.3e-04
Identity = 39/158 (24.68%), Postives = 77/158 (48.73%), Query Frame = 0

Query: 14  YNRITLDLNNIGKAVSDENKVIILLNSLPNSYNEVKTTIK-YGRDSLTMEIVLNALRSRD 73
           ++ +  +L   G  + + +K+  LL +LP+ Y+ + T I+    ++LT+  V N L  ++
Sbjct: 121 FDELISELLAAGAKIEEMDKISHLLITLPSCYDGIITAIETLSEENLTLAFVKNRLLDQE 180

Query: 74  LEMKK----AKEKIMRLWIARGRTEKKS-------SKGTSSSRGRSKSQGKTKRCYYCQK 133
           +++K       +K+M   +       K+       +K     +G SK + K   C++C +
Sbjct: 181 IKIKNDHNDTSKKVMNAIVHNNNNTYKNNLFKNRVTKPKKIFKGNSKYKVK---CHHCGR 240

Query: 134 EGHIRRNCYELKNKRKNEGKKEEEPHNVNVSEGYDFAV 160
           EGHI+++C+  K    N+ K+ E+      S G  F V
Sbjct: 241 EGHIKKDCFHYKRILNNKNKENEKQVQTATSHGIAFMV 275

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
TYK25306.11.6e-3251.41putative gag-pol polyprotein [Cucumis melo var. makuwa][more]
KAA0050719.11.6e-3251.41putative gag-pol polyprotein [Cucumis melo var. makuwa][more]
KAA0043826.12.7e-3251.69putative gag-pol polyprotein [Cucumis melo var. makuwa][more]
KAA0067607.12.3e-3151.76retrotransposon protein, putative, Ty1-copia subclass [Cucumis melo var. makuwa]... [more]
KAA0047995.15.2e-3151.12retrotransposon protein, putative, Ty1-copia sub-class [Cucumis melo var. makuwa... [more]
Match NameE-valueIdentityDescription
P109781.9e-1232.18Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Nicotiana tabacum... [more]
P041467.3e-0424.68Copia protein OS=Drosophila melanogaster OX=7227 GN=GIP PE=1 SV=3[more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001878Zinc finger, CCHC-typeSMARTSM00343c2hcfinal6coord: 115..131
e-value: 0.003
score: 26.7
IPR001878Zinc finger, CCHC-typePROSITEPS50158ZF_CCHCcoord: 115..129
score: 9.504374
NoneNo IPR availableGENE3D4.10.60.10coord: 87..146
e-value: 6.4E-7
score: 31.1
NoneNo IPR availablePFAMPF14223Retrotran_gag_2coord: 1..76
e-value: 2.4E-12
score: 46.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 92..113
IPR036875Zinc finger, CCHC-type superfamilySUPERFAMILY57756Retrovirus zinc finger-like domainscoord: 98..137

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0009159.1Lag0009159.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0008270 zinc ion binding