Lag0008809 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0008809
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptiontRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like
Locationchr9: 30518074 .. 30522256 (+)
RNA-Seq ExpressionLag0008809
SyntenyLag0008809
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGATCCCCTTCTACTTCTCAGCTCCATCTCAAATCAATATTCTCTTTCCATAAGTCTCTCATGGCTTCCTCTTTCACCCAAACCCTTAAACCTGATTTCCCTCAAGACCACGACGACGACCTTCTCTGCTCCGATCCGCAGCAACAGCAACAAGAGGAATGCTCGCCTCTTACCAAATCCGACGACTCATCACTTCTCTTGGGGTCGCCGAGTGGATATTTGAATGGCGAGTCGCGAATTGAGCGAGCCTGGGCTCACTGGTCGAAGCTCGGTCGGCCGAAGTTGATTGTTGCTCCGATGGTTGATAATTCGGAGCTACCCTTCCGTATGCTCTGCAGGAAATATGGTGCCGAGGCGGCGTATACGCCTATGCTCCACTCACGGATCTTTACCGAGAACGAGAAGTATCGGAACATGGAATTTACCACTTGCCAGGTACATGAAAAGTTCTAGCTAATTATGGATCAGAAGTTTTTTAATGTTGCTATTCTTTCTGTATTTCTGTTAATCTTTATGCTTCGTTGTTTTTTTAAATTTGGAATGATTTGTAGGAGGACCGCCCATTATTTGTTCAATTTTGTGCCAATGATCCCGATGTTTTGTTGGAAGCTGCTAGAAGAGTGGAGCCATATTGTGATTACGTCGATCTTAACCTCGGGTAATCTCTATGATCCTCTCTCTAATTCGTATCTATTGGGCAATTCATCCGAATGTTTGCATTCCTTATGATCTTATATCCCAAATGCTTCTCGTTGGTTGCTTGAGTGAAAATTTGTTTTCCGAAAGATTTTTTATTGGTTAAGATTGTGGAAACTGTCCCAAGTACTTGCAATTAGAAGCTTGAATTTGTTACATCAATTGAAAGTGACTAGTTATTGTGTTCATAAACTGTTATATGTTTCTTGAGTCCTGTTAAGTTGTTTCACTTAAAGCTGATACCAGAAAAGTGCTTTCTTGGTCACTCAACATAAGGTTAGCAAACCATTAGGGTTTTGGTTCAACTTGGCTCTAAATGTTAACTAATGACACCGGAAACATGCATAAACTAACATCTTGTATGGACTGCAAAGTTGATAGTTGGTTGAGGTTGAGTGACAGTTTCGATATTAAGTGGGAATGATAGCCTATTTGGTTGATAGTTGTTTTGGAGTTAAGACATGTCAATGTTTCAGAGATGTTATTAGTTTAACATAGTTGCAGTCAGGATACCATATTGAGTCAGACACATACATGCTCCCTGTTTTGCTGCAAGGAGCACGTTTTTTGTGGTAGCTGGTACGAAATTTCTATGCTCTTGGTTGAAGGTCCTAGCAAAGTGTAGTTTTGGAGAGCAATTGAAGATAGATTCAGGAGGAAGTTATGAAAATGGAAAATTTTGTGCCTGTTTTAAGGGTGAACTTGAAGAATTGACTCTGTGTACCCTAATTCGAGGAAGCTTACCAACCTATTTAATGTCAAGCTTTCCTATTAGTAAGGAAAGTAACGTTGGATTTTGAAGAAGATGAGGAATTTCTATAGAATCGTAGCATGGAAGGGAAGTTGTCATTTGGTTGTAATTTTAAGGAATCTAGCCTCTTCTGAAAAATGATTATCAATGTAAAAATTTTGGGACAAACTTTTGGAAGGCTTAATCCTAACTAGAATCAACTATAGTTGATCAAGAATTTCTTTGAGGTAAGATGACACCTTGGGAAATGTTATTGTAACAAACAGATCGTTGGAATGACATGACTAGAAATTTTTCAATGAGATTCATGGAGACTTGATGAATTTTTCAAGTTCCTTCTATAATGTCCCTTGGTTTGAGAGTGCTTTTCTCCTTTTGTATCTTTCATTAGCTCTCAAATCACTGCACCTGAGGAAGAATCCTATACTGATGCTGTAGTTAGTATTTTGAATTCACGGATATAATGAGTGATTTGAGATCAATGAAAAGATTTTATTTTTATTATATGAGAAACAAAGATCTTTCATTGAGAAAAGGTTGAAACTATGAAAGAATACACAAGGACACACAAAAAACAAGCCCGCGAACAAAAAAACACCAGCCCCTAATTACAAAAATGGGTCCCAATTCAAAAGAATAAAGCCAAGCTAATAGCTACAAGATACAAAAGGAGGGGAGCAAATAAAATCCCTATGCAAGAGCAAGGAAGCATATATTGGTGATGTTGATATTTTGATTTTATAGATATACGGCTGTTCAAGTTCCTTTCTATAATCTCCTTTGGTTTGAGAGGCACTCCCCTCCTTTTGTACCTTTTCATGGCTCTCAAATCACTACATAAGAGCAACGAAGCATATACTGATGTTGTTGATATTGTGAATTTATAGATATAATGGGTGGAAATTTCAATGTCAGTCAATATCTCAAGAAAAATTGAAATACCGCAAGAACTCATAAAAATTGTTTAAGACCAATAAATAAACTTTGGTATTTATACAATTAAGTTAAAGAAAATACATCTTTAATGGTACTTGTTTCAATAACTTTAACTGGATTATTAGTATCCCATGCTTAATGAATCTATTAGACTTAAGCTAGGATATAAACATCCAATCGGGATGTGGGAATATAATTAATATTAAGATTTCAAGACATTGAAGATCCTTATTATAGTTATATTAAGACAAATCCAAAGTTCTTTGGACAAAGGCATATAGCTCCTTCGGTTTCTTTGGAAGGAGAGAAGTTCCAGATTATTATTAAATAAGTCATCTTCTTTTGAAAACTTTTGTGACAATGCCCACATTATACGGCCCCAATTTGGAGTGCCTTGCACAAGTATTTTTGTAATATCCAGCCCTTCCTCATCAATATAGTCGGCGTTTTGTTTTTGCTCCACCTCATTCAGCCTTTAGGCTGTTGTCCTTTTTCTCATTAATATGATGGATTTTTCTCACAGAAAAATGGTCCTTAATATGATTAAAATTTAATGTAGCTTTTGCATATGAATACTAACTAATCTAAGAATTAAAATTGGAAAACGCTTTTAAAAGAAATTCACGCATCTTATGTTATTCTTTTTGAGCTATTTTCTGCATTGTTCTCCATTTCAGTCATGTGTCTGAAACTAACAAAACTAACAAAAACAACATTAAGGATAAGCTAAACCATCATGGGTTGGTCCTAATGGTCAATAAGGGCCATAGAAAAAATAAAGGGCTTAGAGGGAATGGGTTCAATTCATGGTGGCCACCTAAATCCTACGAGTTTCCTTGGCAACCAAATGTAGTAGGGTCAGACGGTTGCCCCGTGAGATTAGTCGAGGTGCGCGTAAGCTGGTCCGAACACTCACGGATATAAAAAAAACATTAAGGATAAGCTAATGTCACGTGTGAAGCACACGGAAATAGGTTGCATATCAAATATGTTTTATGGTATTGGTGTCCATATCTTTTACTTATGGCTTCTGAACTTGAATTGAGTGCTCTACTAATTTCATGCAGGTGCCCTCAGCGTATTGCTAGGCGAGGAAATTATGGGGCTTTTCTAATGGATAACTTACCTCTTGTAAAAGCCATAGTAGAGAAGCTGGCTTCCAATCTTCAGGTTCCTGTATCATGCAAAATCCGAATCTTCCCAAATTTACAGGATACAATTAATTATGCAAGAATGTTAGAGGATGCTGGTTGCTCTCTTTTGGCTGTCCATGGCCGCACAAGAGATGAGAAAGATGGTAAGAAATTCCGGGCTAATTGGAGTGCTATTAGTGCTGTCAAAAATGCTGTACGAATTCCAGTTCTTGCCAATGGGAATATAAGGCACATGGAGGATGTTAAGAACTGTCTGCAAGAGACTGGAGTCGAGGGTGTTCTTTCAGCGGAAACCCTTCTTGAGAATCCTGCACTTTTTGCTGGGTTTCGAACTGCAGACTGGATAGAGGGCTGTGAAGAAAATACCAGGGATGGAAACCTTGATCAAGCCGATCTGCTGGTGGACTACTTGAAGCTTTGTGAAAAGTACCCTGTCCCGTGGAGAATGATCCGTTCACACGTACACAAGTTGTTGGGAGATTGGTTCAAGATTCATCCACACATTAGAGAAGACTTTAATGCACAATCCAAGCTCACCTTTGAATTTCTTTACAATATGGTGGAGCGGCTGAGAGAGTTGCAGGTAAAAATTCCTCTTTACATCAAGGATTCTCCTGCACCTGCAATTTCATCCAATGGTTGA

mRNA sequence

ATGAGATCCCCTTCTACTTCTCAGCTCCATCTCAAATCAATATTCTCTTTCCATAAGTCTCTCATGGCTTCCTCTTTCACCCAAACCCTTAAACCTGATTTCCCTCAAGACCACGACGACGACCTTCTCTGCTCCGATCCGCAGCAACAGCAACAAGAGGAATGCTCGCCTCTTACCAAATCCGACGACTCATCACTTCTCTTGGGGTCGCCGAGTGGATATTTGAATGGCGAGTCGCGAATTGAGCGAGCCTGGGCTCACTGGTCGAAGCTCGGTCGGCCGAAGTTGATTGTTGCTCCGATGGTTGATAATTCGGAGCTACCCTTCCGTATGCTCTGCAGGAAATATGGTGCCGAGGCGGCGTATACGCCTATGCTCCACTCACGGATCTTTACCGAGAACGAGAAGTATCGGAACATGGAATTTACCACTTGCCAGGAGGACCGCCCATTATTTGTTCAATTTTGTGCCAATGATCCCGATGTTTTGTTGGAAGCTGCTAGAAGAGTGGAGCCATATTGTGATTACGTCGATCTTAACCTCGGGTGCCCTCAGCGTATTGCTAGGCGAGGAAATTATGGGGCTTTTCTAATGGATAACTTACCTCTTGTAAAAGCCATAGTAGAGAAGCTGGCTTCCAATCTTCAGGTTCCTGTATCATGCAAAATCCGAATCTTCCCAAATTTACAGGATACAATTAATTATGCAAGAATGTTAGAGGATGCTGGTTGCTCTCTTTTGGCTGTCCATGGCCGCACAAGAGATGAGAAAGATGGTAAGAAATTCCGGGCTAATTGGAGTGCTATTAGTGCTGTCAAAAATGCTGTACGAATTCCAGTTCTTGCCAATGGGAATATAAGGCACATGGAGGATGTTAAGAACTGTCTGCAAGAGACTGGAGTCGAGGGTGTTCTTTCAGCGGAAACCCTTCTTGAGAATCCTGCACTTTTTGCTGGGTTTCGAACTGCAGACTGGATAGAGGGCTGTGAAGAAAATACCAGGGATGGAAACCTTGATCAAGCCGATCTGCTGGTGGACTACTTGAAGCTTTGTGAAAAGTACCCTGTCCCGTGGAGAATGATCCGTTCACACGTACACAAGTTGTTGGGAGATTGGTTCAAGATTCATCCACACATTAGAGAAGACTTTAATGCACAATCCAAGCTCACCTTTGAATTTCTTTACAATATGGTGGAGCGGCTGAGAGAGTTGCAGGTAAAAATTCCTCTTTACATCAAGGATTCTCCTGCACCTGCAATTTCATCCAATGGTTGA

Coding sequence (CDS)

ATGAGATCCCCTTCTACTTCTCAGCTCCATCTCAAATCAATATTCTCTTTCCATAAGTCTCTCATGGCTTCCTCTTTCACCCAAACCCTTAAACCTGATTTCCCTCAAGACCACGACGACGACCTTCTCTGCTCCGATCCGCAGCAACAGCAACAAGAGGAATGCTCGCCTCTTACCAAATCCGACGACTCATCACTTCTCTTGGGGTCGCCGAGTGGATATTTGAATGGCGAGTCGCGAATTGAGCGAGCCTGGGCTCACTGGTCGAAGCTCGGTCGGCCGAAGTTGATTGTTGCTCCGATGGTTGATAATTCGGAGCTACCCTTCCGTATGCTCTGCAGGAAATATGGTGCCGAGGCGGCGTATACGCCTATGCTCCACTCACGGATCTTTACCGAGAACGAGAAGTATCGGAACATGGAATTTACCACTTGCCAGGAGGACCGCCCATTATTTGTTCAATTTTGTGCCAATGATCCCGATGTTTTGTTGGAAGCTGCTAGAAGAGTGGAGCCATATTGTGATTACGTCGATCTTAACCTCGGGTGCCCTCAGCGTATTGCTAGGCGAGGAAATTATGGGGCTTTTCTAATGGATAACTTACCTCTTGTAAAAGCCATAGTAGAGAAGCTGGCTTCCAATCTTCAGGTTCCTGTATCATGCAAAATCCGAATCTTCCCAAATTTACAGGATACAATTAATTATGCAAGAATGTTAGAGGATGCTGGTTGCTCTCTTTTGGCTGTCCATGGCCGCACAAGAGATGAGAAAGATGGTAAGAAATTCCGGGCTAATTGGAGTGCTATTAGTGCTGTCAAAAATGCTGTACGAATTCCAGTTCTTGCCAATGGGAATATAAGGCACATGGAGGATGTTAAGAACTGTCTGCAAGAGACTGGAGTCGAGGGTGTTCTTTCAGCGGAAACCCTTCTTGAGAATCCTGCACTTTTTGCTGGGTTTCGAACTGCAGACTGGATAGAGGGCTGTGAAGAAAATACCAGGGATGGAAACCTTGATCAAGCCGATCTGCTGGTGGACTACTTGAAGCTTTGTGAAAAGTACCCTGTCCCGTGGAGAATGATCCGTTCACACGTACACAAGTTGTTGGGAGATTGGTTCAAGATTCATCCACACATTAGAGAAGACTTTAATGCACAATCCAAGCTCACCTTTGAATTTCTTTACAATATGGTGGAGCGGCTGAGAGAGTTGCAGGTAAAAATTCCTCTTTACATCAAGGATTCTCCTGCACCTGCAATTTCATCCAATGGTTGA

Protein sequence

MRSPSTSQLHLKSIFSFHKSLMASSFTQTLKPDFPQDHDDDLLCSDPQQQQQEECSPLTKSDDSSLLLGSPSGYLNGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRMLCRKYGAEAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVKNCLQETGVEGVLSAETLLENPALFAGFRTADWIEGCEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPAPAISSNG
Homology
BLAST of Lag0008809 vs. NCBI nr
Match: XP_038901742.1 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Benincasa hispida])

HSP 1 Score: 802.4 bits (2071), Expect = 1.9e-228
Identity = 392/423 (92.67%), Postives = 401/423 (94.80%), Query Frame = 0

Query: 1   MRSPSTSQLHLKSIFSFHKSLMASSFTQTLKPDFPQDHDDDLLCSDPQQQQQEECSPLTK 60
           MRSP  S LH K+IFSF  SLMASSFTQTLKPD PQD  DD LCSDPQ QQQ+ECS  TK
Sbjct: 1   MRSPYNSHLHFKTIFSFRSSLMASSFTQTLKPDLPQDL-DDRLCSDPQNQQQQECSSFTK 60

Query: 61  SDDSSLLLGSPSGYLNGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRMLCRKYGAEA 120
           S+DS+LLL SPSGYL GESRIERAWAHW KLGRPKLIVAPMVDNSELPFR+LCRKYGAEA
Sbjct: 61  SNDSTLLLASPSGYLCGESRIERAWAHWRKLGRPKLIVAPMVDNSELPFRLLCRKYGAEA 120

Query: 121 AYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLN 180
           AYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLN
Sbjct: 121 AYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLN 180

Query: 181 LGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLE 240
           LGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIF NLQDTINYARMLE
Sbjct: 181 LGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFSNLQDTINYARMLE 240

Query: 241 DAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVKNCLQETG 300
           DAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVR+PVLANGNIRHMEDV NCLQETG
Sbjct: 241 DAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRVPVLANGNIRHMEDVYNCLQETG 300

Query: 301 VEGVLSAETLLENPALFAGFRTADWIEGCEENTRDGNLDQADLLVDYLKLCEKYPVPWRM 360
           VEGVLSAETLLENPALFAGFRTA+WI+G EENTRDGNLDQADLLVDYLK CEKYPVPWRM
Sbjct: 301 VEGVLSAETLLENPALFAGFRTAEWIKGHEENTRDGNLDQADLLVDYLKFCEKYPVPWRM 360

Query: 361 IRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPAPAI 420
           IRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDS A AI
Sbjct: 361 IRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSHASAI 420

Query: 421 SSN 424
           +SN
Sbjct: 421 ASN 422

BLAST of Lag0008809 vs. NCBI nr
Match: XP_022944464.1 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Cucurbita moschata])

HSP 1 Score: 801.6 bits (2069), Expect = 3.3e-228
Identity = 392/424 (92.45%), Postives = 403/424 (95.05%), Query Frame = 0

Query: 1   MRSPSTSQLHLKSIFSFHKSLMASSFTQTLKPDFPQDHDDDLLCSDPQQQQQEECSPLTK 60
           MRSPS S LHLKSI S  KS MASSFTQT   DFPQD  DDLLCSDP  Q Q+ECS LTK
Sbjct: 1   MRSPSNSHLHLKSILSLRKSFMASSFTQT---DFPQDL-DDLLCSDPHTQPQDECSSLTK 60

Query: 61  SDDSSLLLGSPSGYLNGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRMLCRKYGAEA 120
           S+DS+LLLGSPSGYL+GESRIERAWAHWSKLGRPKLIVAPMVDNSELPFR+LCRKYGAEA
Sbjct: 61  SNDSTLLLGSPSGYLSGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEA 120

Query: 121 AYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLN 180
           AYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLN
Sbjct: 121 AYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLN 180

Query: 181 LGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLE 240
           LGCPQRIARRGNYGAFLMDNLPLVKAIVEK+ASNLQVPVSCKIR+FPNLQDTINYARMLE
Sbjct: 181 LGCPQRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLE 240

Query: 241 DAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVKNCLQETG 300
           DAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNA+RIPVLANGNIRHMEDV NCLQETG
Sbjct: 241 DAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNALRIPVLANGNIRHMEDVHNCLQETG 300

Query: 301 VEGVLSAETLLENPALFAGFRTADWIEGCEENTRDGNLDQADLLVDYLKLCEKYPVPWRM 360
           VEGVLSAETLLENPALFAGFRTA W  GCEE++RDGNLDQADLLVDYLKLCEKYPVPWRM
Sbjct: 301 VEGVLSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRM 360

Query: 361 IRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPAPAI 420
           IRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVE+LRELQVKIPLYIKDSPA AI
Sbjct: 361 IRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVEQLRELQVKIPLYIKDSPASAI 420

Query: 421 SSNG 425
           SS G
Sbjct: 421 SSIG 420

BLAST of Lag0008809 vs. NCBI nr
Match: KAG7011024.1 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 800.8 bits (2067), Expect = 5.6e-228
Identity = 392/424 (92.45%), Postives = 402/424 (94.81%), Query Frame = 0

Query: 1   MRSPSTSQLHLKSIFSFHKSLMASSFTQTLKPDFPQDHDDDLLCSDPQQQQQEECSPLTK 60
           MRSPS S LHLKSI S  KS MASSFTQT   DFPQD  DDLLCSDP  Q Q+ECS LTK
Sbjct: 1   MRSPSNSHLHLKSILSLRKSFMASSFTQT---DFPQDL-DDLLCSDPHTQPQDECSSLTK 60

Query: 61  SDDSSLLLGSPSGYLNGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRMLCRKYGAEA 120
           S+DS+LLLGSPSGYL+GESRIERAWAHWSKLGRPKLIVAPMVDNSELPFR+LCRKYGAEA
Sbjct: 61  SNDSTLLLGSPSGYLSGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEA 120

Query: 121 AYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLN 180
           AYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLN
Sbjct: 121 AYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLN 180

Query: 181 LGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLE 240
           LGCPQRIARRGNYGAFLMDNLPLVKAIVEK+ASNLQVPVSCKIR+FPNLQDTI YARMLE
Sbjct: 181 LGCPQRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTITYARMLE 240

Query: 241 DAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVKNCLQETG 300
           DAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNA+RIPVLANGNIRHMEDV NCLQETG
Sbjct: 241 DAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNALRIPVLANGNIRHMEDVHNCLQETG 300

Query: 301 VEGVLSAETLLENPALFAGFRTADWIEGCEENTRDGNLDQADLLVDYLKLCEKYPVPWRM 360
           VEGVLSAETLLENPALFAGFRTA W  GCEE++RDGNLDQADLLVDYLKLCEKYPVPWRM
Sbjct: 301 VEGVLSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRM 360

Query: 361 IRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPAPAI 420
           IRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPA AI
Sbjct: 361 IRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPASAI 420

Query: 421 SSNG 425
           SS G
Sbjct: 421 SSIG 420

BLAST of Lag0008809 vs. NCBI nr
Match: XP_022986543.1 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Cucurbita maxima])

HSP 1 Score: 799.3 bits (2063), Expect = 1.6e-227
Identity = 391/424 (92.22%), Postives = 401/424 (94.58%), Query Frame = 0

Query: 1   MRSPSTSQLHLKSIFSFHKSLMASSFTQTLKPDFPQDHDDDLLCSDPQQQQQEECSPLTK 60
           MRSPS S LHLKSI S  KS MASS  QT   DFPQD  DDLLCSDP  Q+Q+ECS LTK
Sbjct: 1   MRSPSNSHLHLKSILSLRKSFMASSCAQT---DFPQDL-DDLLCSDPHTQRQDECSSLTK 60

Query: 61  SDDSSLLLGSPSGYLNGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRMLCRKYGAEA 120
           S+DS+LLLGSPSGYLNGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFR+LCRKYGAEA
Sbjct: 61  SNDSTLLLGSPSGYLNGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEA 120

Query: 121 AYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLN 180
           AYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLN
Sbjct: 121 AYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLN 180

Query: 181 LGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLE 240
           LGCPQRIARRGNYGAFLMDNLPLVKAIVEK+ASNLQVPVSCKIR+FPNLQDTINYARMLE
Sbjct: 181 LGCPQRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLE 240

Query: 241 DAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVKNCLQETG 300
           DAGCSLLAVHGRTRDEKDGKKFRANWSAI AVKNA+RIPVLANGNIRHMEDV NCLQETG
Sbjct: 241 DAGCSLLAVHGRTRDEKDGKKFRANWSAIRAVKNALRIPVLANGNIRHMEDVNNCLQETG 300

Query: 301 VEGVLSAETLLENPALFAGFRTADWIEGCEENTRDGNLDQADLLVDYLKLCEKYPVPWRM 360
           VEGVLSAETLLENPALFAGFRTA W  GCEE++RDGNLDQADLLVDYLKLCEKYPVPWRM
Sbjct: 301 VEGVLSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRM 360

Query: 361 IRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPAPAI 420
           IRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPA AI
Sbjct: 361 IRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPASAI 420

Query: 421 SSNG 425
           SS G
Sbjct: 421 SSIG 420

BLAST of Lag0008809 vs. NCBI nr
Match: XP_023512932.1 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 796.2 bits (2055), Expect = 1.4e-226
Identity = 390/424 (91.98%), Postives = 401/424 (94.58%), Query Frame = 0

Query: 1   MRSPSTSQLHLKSIFSFHKSLMASSFTQTLKPDFPQDHDDDLLCSDPQQQQQEECSPLTK 60
           MRSPS S LHLKSI S  KS MASS  QT   D PQD  DDLLCSDP  Q+Q+ECS LTK
Sbjct: 1   MRSPSNSHLHLKSILSLRKSFMASSRAQT---DLPQDL-DDLLCSDPHTQRQDECSSLTK 60

Query: 61  SDDSSLLLGSPSGYLNGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRMLCRKYGAEA 120
           S+DS+LLLGSPSGYL+GESRIERAWAHWSKLGRPKLIVAPMVDNSELPFR+LCRKYGAEA
Sbjct: 61  SNDSTLLLGSPSGYLSGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEA 120

Query: 121 AYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLN 180
           AYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLN
Sbjct: 121 AYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLN 180

Query: 181 LGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLE 240
           LGCPQRIARRGNYGAFLMDNLPLVKAIVEK+ASNLQVPVSCKIR+FPNLQDTINYARMLE
Sbjct: 181 LGCPQRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLE 240

Query: 241 DAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVKNCLQETG 300
           DAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNA+RIPVLANGNIRHMEDV NCLQETG
Sbjct: 241 DAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNALRIPVLANGNIRHMEDVHNCLQETG 300

Query: 301 VEGVLSAETLLENPALFAGFRTADWIEGCEENTRDGNLDQADLLVDYLKLCEKYPVPWRM 360
           VEGVLSAETLLENPALFAGFRTA W  GCEE++RDGNLDQADLLVDYLKLCEKYPVPWRM
Sbjct: 301 VEGVLSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRM 360

Query: 361 IRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPAPAI 420
           IRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPA AI
Sbjct: 361 IRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPASAI 420

Query: 421 SSNG 425
           SS G
Sbjct: 421 SSIG 420

BLAST of Lag0008809 vs. ExPASy Swiss-Prot
Match: Q8K582 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Rattus norvegicus OX=10116 GN=Dus1l PE=2 SV=1)

HSP 1 Score: 296.6 bits (758), Expect = 4.5e-79
Identity = 142/298 (47.65%), Postives = 195/298 (65.44%), Query Frame = 0

Query: 88  WSK-LGRPKLIVAPMVDNSELPFRMLCRKYGAEAAYTPMLHSRIFTENEKYR--NMEFTT 147
           WS+ LG  + +VAPMVD SEL +R+L R++GA+  YTPMLH+++F  +  YR  N+    
Sbjct: 10  WSRALGGARHVVAPMVDQSELAWRLLSRRHGAQLCYTPMLHAQVFVRDANYRKENLYCDV 69

Query: 148 CQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFLMDNLPLV 207
           C EDRPL VQFCANDP+V ++AA   + YCD +DLNLGCPQ IA+RG YGAFL +   L+
Sbjct: 70  CPEDRPLIVQFCANDPEVFVQAALLAQDYCDAIDLNLGCPQMIAKRGRYGAFLQEEWDLL 129

Query: 208 KAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRA 267
           + ++      L VPV+CKIR+FP +  T+ YA+MLE AGC LL VHGRT+++K      A
Sbjct: 130 QRMILLAHERLSVPVTCKIRVFPEIDKTVRYAQMLEKAGCQLLTVHGRTKEQKGPMAGTA 189

Query: 268 NWSAISAVKNAVRIPVLANGNIRHMEDVKNCLQETGVEGVLSAETLLENPALFAGFRTAD 327
           +W  I AV+ AV IPV ANGNIR ++DV+ C+Q+TGV+GV+SAE  L NPALF G   A 
Sbjct: 190 SWEHIKAVRKAVGIPVFANGNIRCLQDVERCIQDTGVQGVMSAEGNLHNPALFEGRSPAV 249

Query: 328 WIEGCEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPHIRED 383
           W                +L  +YL +  ++P P   +R+H+ KL     ++H  +RE+
Sbjct: 250 W----------------ELADEYLDIVRQHPCPLSYVRAHLFKLWHHTLQVHQQLREE 291

BLAST of Lag0008809 vs. ExPASy Swiss-Prot
Match: Q8C2P3 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Mus musculus OX=10090 GN=Dus1l PE=2 SV=1)

HSP 1 Score: 295.8 bits (756), Expect = 7.7e-79
Identity = 141/298 (47.32%), Postives = 196/298 (65.77%), Query Frame = 0

Query: 88  WSK-LGRPKLIVAPMVDNSELPFRMLCRKYGAEAAYTPMLHSRIFTENEKYR--NMEFTT 147
           WS+ LG  + +VAPMVD SEL +R+L R++GA+  YTPMLH+++F  +  YR  N+    
Sbjct: 10  WSRTLGGARHVVAPMVDQSELAWRLLSRRHGAQLCYTPMLHAQVFVRDANYRKENLYCDV 69

Query: 148 CQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFLMDNLPLV 207
           C EDRPL VQFCANDP+V ++AA   + YCD +DLNLGCPQ IA+RG+YGAFL +   L+
Sbjct: 70  CPEDRPLIVQFCANDPEVFVQAALLAQDYCDAIDLNLGCPQMIAKRGHYGAFLQEEWDLL 129

Query: 208 KAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRA 267
           + ++      L VPV+CKIR+FP +  T+ YA+MLE AGC LL VHGRT+++K      A
Sbjct: 130 QRMILLAHERLSVPVTCKIRVFPEIDKTVRYAQMLEKAGCQLLTVHGRTKEQKGPMAGTA 189

Query: 268 NWSAISAVKNAVRIPVLANGNIRHMEDVKNCLQETGVEGVLSAETLLENPALFAGFRTAD 327
           +W  I AV+ AV IPV ANGNI+ ++DV+ C+Q+TGV+GV+SAE  L NPALF G   A 
Sbjct: 190 SWEHIKAVRKAVGIPVFANGNIQCLQDVERCIQDTGVQGVMSAEGNLHNPALFEGRSPAV 249

Query: 328 WIEGCEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPHIRED 383
           W                +L  +YL +  ++P P   +R+H+ KL     ++H  +RE+
Sbjct: 250 W----------------ELAEEYLDIVRQHPCPLSYVRAHLFKLWHHTLQVHQQLREE 291

BLAST of Lag0008809 vs. ExPASy Swiss-Prot
Match: Q6P1R4 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Homo sapiens OX=9606 GN=DUS1L PE=1 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 2.9e-78
Identity = 141/298 (47.32%), Postives = 195/298 (65.44%), Query Frame = 0

Query: 88  WSK-LGRPKLIVAPMVDNSELPFRMLCRKYGAEAAYTPMLHSRIFTENEKYR--NMEFTT 147
           WS+ L   + +VAPMVD SEL +R+L R++GA+  YTPMLH+++F  +  YR  N+    
Sbjct: 10  WSRTLRGARHVVAPMVDQSELAWRLLSRRHGAQLCYTPMLHAQVFVRDANYRKENLYCEV 69

Query: 148 CQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFLMDNLPLV 207
           C EDRPL VQFCANDP+V ++AA   + YCD +DLNLGCPQ IA+RG+YGAFL D   L+
Sbjct: 70  CPEDRPLIVQFCANDPEVFVQAALLAQDYCDAIDLNLGCPQMIAKRGHYGAFLQDEWDLL 129

Query: 208 KAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRA 267
           + ++      L VPV+CKIR+FP +  T+ YA+MLE AGC LL VHGRT+++K      A
Sbjct: 130 QRMILLAHEKLSVPVTCKIRVFPEIDKTVRYAQMLEKAGCQLLTVHGRTKEQKGPLSGAA 189

Query: 268 NWSAISAVKNAVRIPVLANGNIRHMEDVKNCLQETGVEGVLSAETLLENPALFAGFRTAD 327
           +W  I AV+ AV IPV ANGNI+ ++DV+ CL++TGV+GV+SAE  L NPALF G   A 
Sbjct: 190 SWEHIKAVRKAVAIPVFANGNIQCLQDVERCLRDTGVQGVMSAEGNLHNPALFEGRSPAV 249

Query: 328 WIEGCEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPHIRED 383
           W                +L  +YL +  ++P P   +R+H+ KL     ++H  +RE+
Sbjct: 250 W----------------ELAEEYLDIVREHPCPLSYVRAHLFKLWHHTLQVHQELREE 291

BLAST of Lag0008809 vs. ExPASy Swiss-Prot
Match: Q9HGN6 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)] OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=dus1 PE=3 SV=1)

HSP 1 Score: 263.1 bits (671), Expect = 5.5e-69
Identity = 137/302 (45.36%), Postives = 194/302 (64.24%), Query Frame = 0

Query: 88  WSKLGRPKLIVAPMVDNSELPFRMLCRKYGAEAAYTPMLHSRIFTENEKYRNMEFT--TC 147
           ++K+GRPK I+APMVD SELP+R+L R+ GA+  Y+PM HSR+F E+E YRN  F+  T 
Sbjct: 12  YNKIGRPKRILAPMVDQSELPWRILARRSGADLCYSPMFHSRLFGESEDYRNKVFSTRTI 71

Query: 148 QEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFLMDNLPLVK 207
            E+RPL +QFC NDP+++L+AA+   PYCD VD+NLGCPQ IA++G YG+FL +N  L++
Sbjct: 72  PEERPLIIQFCGNDPEIMLKAAKIAAPYCDAVDVNLGCPQGIAKKGKYGSFLQENWNLIE 131

Query: 208 AIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRAN 267
           +I+ KL + L +PV+ KIRIFP+ Q T++YA+M+  AG S+LAVHGR R++K      A+
Sbjct: 132 SIITKLHTELSIPVTAKIRIFPDPQKTLDYAKMILKAGASILAVHGRLREQKGHFTGIAD 191

Query: 268 WSAISAV-KNAVRIPVL-ANGNIRHMEDVKNCLQETGVEGVLSAETLLENPALFAGFRTA 327
           W  I  + KN     VL ANGNI H +D+  C++ TGV+GVLSAE  L NP +F      
Sbjct: 192 WEQIQMLRKNLPSETVLFANGNILHAQDIDRCIKYTGVDGVLSAEGSLYNPRIFLP---- 251

Query: 328 DWIEGCEENTRDGNLDQADLLVDYLKLCEKYPV-----PWRMIRSHVHKLLGDWFKIHPH 381
                    T    +D  D+  +YL +  ++ +         I+ H+ KL+     IH  
Sbjct: 252 ---PSSPLMTLYPRID--DMCEEYLNIIREFKLESDYSSLSAIKGHLFKLMRPLLSIHTD 304

BLAST of Lag0008809 vs. ExPASy Swiss-Prot
Match: P53759 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)] OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=DUS1 PE=1 SV=1)

HSP 1 Score: 248.4 bits (633), Expect = 1.4e-64
Identity = 130/315 (41.27%), Postives = 189/315 (60.00%), Query Frame = 0

Query: 88  WSKLGRPKLIVAPMVDNSELPFRMLCRKYGAEAAYTPMLHSRIFTENEKYRNMEFTT--- 147
           + K+GRP  IVAPMVD SEL +R+L R+YGA  AYTPMLH+++F  ++KYR   +++   
Sbjct: 23  FDKIGRPTRIVAPMVDQSELAWRILSRRYGATLAYTPMLHAKLFATSKKYREDNWSSLDG 82

Query: 148 CQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFLMDNLPLV 207
              DRPL VQFCANDP+ LL AA+ VE  CD VDLNLGCPQ IA++G+YG+FLM+   L+
Sbjct: 83  SSVDRPLVVQFCANDPEYLLAAAKLVEDKCDAVDLNLGCPQGIAKKGHYGSFLMEEWDLI 142

Query: 208 KAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRA 267
             ++  L  NL+VPV+ KIRIF + + ++NYA+M+ DAG   L VHGR R++K  K   A
Sbjct: 143 HNLINTLHKNLKVPVTAKIRIFDDCEKSLNYAKMVLDAGAQFLTVHGRVREQKGQKTGLA 202

Query: 268 NWSAISAVKNAV--RIPVLANGNIRHMEDVKNCLQETGVEGVLSAETLLENPALFAGFRT 327
           NW  I  +++ +       ANGNI + ED+  C++  G + V+SAE  L NP +F   +T
Sbjct: 203 NWETIKYLRDNLPKETVFFANGNILYPEDISRCMEHIGADAVMSAEGNLYNPGVFNVGQT 262

Query: 328 ADWIEGCEENTRDGNLDQADLLV----DYLKLCEKYPVPWRMIRSHVHKLLGDWFKIHPH 387
                      ++    + D ++      +K C++       ++SH  K+L  +   H  
Sbjct: 263 ---------KNKEKIFPRVDKIIREYFQIVKECQESKASKTAMKSHFFKILRPFLPHHTD 322

Query: 388 IREDF-NAQSKLTFE 393
           IR       +K T+E
Sbjct: 323 IRSTLATMNAKATWE 328

BLAST of Lag0008809 vs. ExPASy TrEMBL
Match: A0A6J1FWR0 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucurbita moschata OX=3662 GN=LOC111448913 PE=4 SV=1)

HSP 1 Score: 801.6 bits (2069), Expect = 1.6e-228
Identity = 392/424 (92.45%), Postives = 403/424 (95.05%), Query Frame = 0

Query: 1   MRSPSTSQLHLKSIFSFHKSLMASSFTQTLKPDFPQDHDDDLLCSDPQQQQQEECSPLTK 60
           MRSPS S LHLKSI S  KS MASSFTQT   DFPQD  DDLLCSDP  Q Q+ECS LTK
Sbjct: 1   MRSPSNSHLHLKSILSLRKSFMASSFTQT---DFPQDL-DDLLCSDPHTQPQDECSSLTK 60

Query: 61  SDDSSLLLGSPSGYLNGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRMLCRKYGAEA 120
           S+DS+LLLGSPSGYL+GESRIERAWAHWSKLGRPKLIVAPMVDNSELPFR+LCRKYGAEA
Sbjct: 61  SNDSTLLLGSPSGYLSGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEA 120

Query: 121 AYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLN 180
           AYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLN
Sbjct: 121 AYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLN 180

Query: 181 LGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLE 240
           LGCPQRIARRGNYGAFLMDNLPLVKAIVEK+ASNLQVPVSCKIR+FPNLQDTINYARMLE
Sbjct: 181 LGCPQRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLE 240

Query: 241 DAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVKNCLQETG 300
           DAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNA+RIPVLANGNIRHMEDV NCLQETG
Sbjct: 241 DAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNALRIPVLANGNIRHMEDVHNCLQETG 300

Query: 301 VEGVLSAETLLENPALFAGFRTADWIEGCEENTRDGNLDQADLLVDYLKLCEKYPVPWRM 360
           VEGVLSAETLLENPALFAGFRTA W  GCEE++RDGNLDQADLLVDYLKLCEKYPVPWRM
Sbjct: 301 VEGVLSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRM 360

Query: 361 IRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPAPAI 420
           IRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVE+LRELQVKIPLYIKDSPA AI
Sbjct: 361 IRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVEQLRELQVKIPLYIKDSPASAI 420

Query: 421 SSNG 425
           SS G
Sbjct: 421 SSIG 420

BLAST of Lag0008809 vs. ExPASy TrEMBL
Match: A0A6J1JGU3 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucurbita maxima OX=3661 GN=LOC111484251 PE=4 SV=1)

HSP 1 Score: 799.3 bits (2063), Expect = 7.9e-228
Identity = 391/424 (92.22%), Postives = 401/424 (94.58%), Query Frame = 0

Query: 1   MRSPSTSQLHLKSIFSFHKSLMASSFTQTLKPDFPQDHDDDLLCSDPQQQQQEECSPLTK 60
           MRSPS S LHLKSI S  KS MASS  QT   DFPQD  DDLLCSDP  Q+Q+ECS LTK
Sbjct: 1   MRSPSNSHLHLKSILSLRKSFMASSCAQT---DFPQDL-DDLLCSDPHTQRQDECSSLTK 60

Query: 61  SDDSSLLLGSPSGYLNGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRMLCRKYGAEA 120
           S+DS+LLLGSPSGYLNGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFR+LCRKYGAEA
Sbjct: 61  SNDSTLLLGSPSGYLNGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEA 120

Query: 121 AYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLN 180
           AYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLN
Sbjct: 121 AYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLN 180

Query: 181 LGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLE 240
           LGCPQRIARRGNYGAFLMDNLPLVKAIVEK+ASNLQVPVSCKIR+FPNLQDTINYARMLE
Sbjct: 181 LGCPQRIARRGNYGAFLMDNLPLVKAIVEKVASNLQVPVSCKIRVFPNLQDTINYARMLE 240

Query: 241 DAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVKNCLQETG 300
           DAGCSLLAVHGRTRDEKDGKKFRANWSAI AVKNA+RIPVLANGNIRHMEDV NCLQETG
Sbjct: 241 DAGCSLLAVHGRTRDEKDGKKFRANWSAIRAVKNALRIPVLANGNIRHMEDVNNCLQETG 300

Query: 301 VEGVLSAETLLENPALFAGFRTADWIEGCEENTRDGNLDQADLLVDYLKLCEKYPVPWRM 360
           VEGVLSAETLLENPALFAGFRTA W  GCEE++RDGNLDQADLLVDYLKLCEKYPVPWRM
Sbjct: 301 VEGVLSAETLLENPALFAGFRTAQWTAGCEESSRDGNLDQADLLVDYLKLCEKYPVPWRM 360

Query: 361 IRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPAPAI 420
           IRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPA AI
Sbjct: 361 IRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPASAI 420

Query: 421 SSNG 425
           SS G
Sbjct: 421 SSIG 420

BLAST of Lag0008809 vs. ExPASy TrEMBL
Match: A0A1S3CHU6 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucumis melo OX=3656 GN=LOC103501114 PE=4 SV=1)

HSP 1 Score: 795.4 bits (2053), Expect = 1.1e-226
Identity = 389/425 (91.53%), Postives = 402/425 (94.59%), Query Frame = 0

Query: 1   MRSPSTSQLHLKSIFSFHKSLMASSFTQTLKPDFPQDHDDDLLCSDPQQQQQEECSPLT- 60
           MRS S S L+LK+IFS   S MASSFTQTLKPD P    DDLLCS PQ+Q+Q+E S LT 
Sbjct: 1   MRSLSNSHLYLKTIFSLRNSFMASSFTQTLKPDLPPQDLDDLLCSHPQKQRQDESSSLTG 60

Query: 61  -KSDDSSLLLGSPSGYLNGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRMLCRKYGA 120
             S+DS LLLGSP+GYL+GESRIERAWAHW+KLGRPKLIVAPMVDNSELPFR+LCRKYGA
Sbjct: 61  SNSNDSKLLLGSPTGYLSGESRIERAWAHWTKLGRPKLIVAPMVDNSELPFRLLCRKYGA 120

Query: 121 EAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180
           +AAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD
Sbjct: 121 DAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVD 180

Query: 181 LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARM 240
           LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARM
Sbjct: 181 LNLGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARM 240

Query: 241 LEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVKNCLQE 300
           LEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDV NCLQE
Sbjct: 241 LEDAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVNNCLQE 300

Query: 301 TGVEGVLSAETLLENPALFAGFRTADWIEGCEENTRDGNLDQADLLVDYLKLCEKYPVPW 360
           TGVEGVLSAETLLENPALFAGFRTA+WIEG EENTRDGNLDQADLLVDYLKLCEKYPVPW
Sbjct: 301 TGVEGVLSAETLLENPALFAGFRTAEWIEGQEENTRDGNLDQADLLVDYLKLCEKYPVPW 360

Query: 361 RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPAP 420
           RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVK+PLYIKDS  P
Sbjct: 361 RMIRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKMPLYIKDSHVP 420

Query: 421 AISSN 424
           AISSN
Sbjct: 421 AISSN 425

BLAST of Lag0008809 vs. ExPASy TrEMBL
Match: A0A6J1D4R6 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Momordica charantia OX=3673 GN=LOC111017278 PE=4 SV=1)

HSP 1 Score: 793.9 bits (2049), Expect = 3.3e-226
Identity = 379/424 (89.39%), Postives = 406/424 (95.75%), Query Frame = 0

Query: 1   MRSPSTSQLHLKSIFSFHKSLMASSFTQTLKPDFPQDHDDDLLCSDPQQQQQEECSPLTK 60
           MRS + S LHLKS+FS H SLMASSF+QT+KPD P +H DDLLCS+ QQQQ+EECS +TK
Sbjct: 1   MRSLAISHLHLKSVFSLHSSLMASSFSQTIKPDLP-EHLDDLLCSNQQQQQREECSSVTK 60

Query: 61  SDDSSLLLGSPSGYLNGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRMLCRKYGAEA 120
           SD+ SLLLGSP+GYL+G+SRIERAWAHWSKLGRPKLIVAPMVDNSELPFR+LCRKYGAEA
Sbjct: 61  SDEPSLLLGSPNGYLSGDSRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEA 120

Query: 121 AYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLN 180
           AYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLN
Sbjct: 121 AYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLN 180

Query: 181 LGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLE 240
           LGCPQRIARRGNYGAFLMDNLPLVKAIV+KL+SNLQVPVSCKIRIFPNLQDT+NYARMLE
Sbjct: 181 LGCPQRIARRGNYGAFLMDNLPLVKAIVQKLSSNLQVPVSCKIRIFPNLQDTLNYARMLE 240

Query: 241 DAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVKNCLQETG 300
           DAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAV+IPVLANGNIRHMEDV NCLQETG
Sbjct: 241 DAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVQIPVLANGNIRHMEDVNNCLQETG 300

Query: 301 VEGVLSAETLLENPALFAGFRTADWIEGCEENTRDGNLDQADLLVDYLKLCEKYPVPWRM 360
           VEGVLSAETLLENPALFAGFRTA+W+EGC+EN RDGNLDQA LLV+YLKLCE+YPVPWR+
Sbjct: 301 VEGVLSAETLLENPALFAGFRTAEWVEGCDENCRDGNLDQAALLVEYLKLCEQYPVPWRI 360

Query: 361 IRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSPAPAI 420
           IRSHVHK+LGDWFKIHPH+REDFNAQ KLTFEFLY MV+RLRELQVKIPLY+KDSPA  I
Sbjct: 361 IRSHVHKMLGDWFKIHPHVREDFNAQFKLTFEFLYIMVDRLRELQVKIPLYLKDSPASEI 420

Query: 421 SSNG 425
           SSNG
Sbjct: 421 SSNG 423

BLAST of Lag0008809 vs. ExPASy TrEMBL
Match: A0A6J1G672 (tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucurbita moschata OX=3662 GN=LOC111451248 PE=4 SV=1)

HSP 1 Score: 783.1 bits (2021), Expect = 5.9e-223
Identity = 385/425 (90.59%), Postives = 404/425 (95.06%), Query Frame = 0

Query: 1   MRSPSTSQLHLKSIFSFHKSLMASSFTQTLKPDFPQDHDDDLLCSDPQQQQQEECSPLTK 60
           MRS STS LHLK+I S +KSLMASS TQTLKPDFPQD  DDLLCSDPQQ QQEE    +K
Sbjct: 1   MRSLSTSNLHLKAIISLYKSLMASSVTQTLKPDFPQDL-DDLLCSDPQQHQQEE----SK 60

Query: 61  SDDSSLLLGSPSGYLNGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRMLCRKYGAEA 120
           SDD SLL+ S SGYLNG SRIERAWAHWSKLGRPKLIVAPMVDNSELPFR+LCRKYGAEA
Sbjct: 61  SDDPSLLVVSRSGYLNGGSRIERAWAHWSKLGRPKLIVAPMVDNSELPFRLLCRKYGAEA 120

Query: 121 AYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLN 180
           AYTPMLHSRIFTEN+KYRN+EFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLN
Sbjct: 121 AYTPMLHSRIFTENDKYRNVEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLN 180

Query: 181 LGCPQRIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLE 240
           LGCPQRIA+RGNYGAFLMDNLPLVKAIVEKLASNL+VPVSCKIRIFPNLQDTINYARMLE
Sbjct: 181 LGCPQRIAKRGNYGAFLMDNLPLVKAIVEKLASNLEVPVSCKIRIFPNLQDTINYARMLE 240

Query: 241 DAGCSLLAVHGRTRDEKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVKNCLQETG 300
           +AGCSLLAVHGRTRDEKDGKKFRANWSAI+AVKNAVRIPVLANGNIRHMEDV +CL+ET 
Sbjct: 241 EAGCSLLAVHGRTRDEKDGKKFRANWSAITAVKNAVRIPVLANGNIRHMEDVYDCLKETR 300

Query: 301 VEGVLSAETLLENPALFAGFRTADWIEGCEENTRDGNLDQADLLVDYLKLCEKYPVPWRM 360
           VEGVLSAETLLENPALFAGFRTA+W+EGCEE+TRDGNLDQADLLVDYLKLCEKYPVPWR+
Sbjct: 301 VEGVLSAETLLENPALFAGFRTAEWVEGCEESTRDGNLDQADLLVDYLKLCEKYPVPWRI 360

Query: 361 IRSHVHKLLGDWFKIHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKD-SPAPA 420
           IRSHVHKLLGDWFK+HPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKD SPA A
Sbjct: 361 IRSHVHKLLGDWFKLHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIKDSSPASA 420

Query: 421 ISSNG 425
           I +NG
Sbjct: 421 ILANG 420

BLAST of Lag0008809 vs. TAIR 10
Match: AT5G67220.1 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 610.5 bits (1573), Expect = 1.0e-174
Identity = 294/399 (73.68%), Postives = 337/399 (84.46%), Query Frame = 0

Query: 19  KSLMASSFTQTLKPDFPQDHD---DDLLCSDPQQQQQEECSPLTKSDDSSLLLGSPSGYL 78
           KSL++ +   T  PD   D     DD+LCS+ +  Q EE       D +   LGSPS  L
Sbjct: 15  KSLISQTRAMTQNPDPKPDPSQVLDDILCSEQRDGQIEE-----TVDTAPASLGSPSRVL 74

Query: 79  NGESRIERAWAHWSKLGRPKLIVAPMVDNSELPFRMLCRKYGAEAAYTPMLHSRIFTENE 138
           + ++R+ERAWAHW KLGRPK IVAPMVDNSELPFR+LC+KYGA+AAYTPMLHSRIFTE E
Sbjct: 75  SIDTRVERAWAHWKKLGRPKYIVAPMVDNSELPFRLLCQKYGAQAAYTPMLHSRIFTETE 134

Query: 139 KYRNMEFTTCQEDRPLFVQFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGA 198
           KYRN EFTTC+EDRPLFVQFCANDPD LLEAA+RVEPYCDYVD+NLGCPQRIARRGNYGA
Sbjct: 135 KYRNQEFTTCKEDRPLFVQFCANDPDTLLEAAKRVEPYCDYVDINLGCPQRIARRGNYGA 194

Query: 199 FLMDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRD 258
           FLMDNLPLVK++VEKLA NL VPVSCKIRIFPNL+DT+ YA+MLEDAGCSLLAVHGRTRD
Sbjct: 195 FLMDNLPLVKSLVEKLAQNLNVPVSCKIRIFPNLEDTLKYAKMLEDAGCSLLAVHGRTRD 254

Query: 259 EKDGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVKNCLQETGVEGVLSAETLLENPA 318
           EKDGKKFRA+WSAI  VKNA+RIPVLANGN+R +EDV NC++ETGVEGVLSAETLLENPA
Sbjct: 255 EKDGKKFRADWSAIKEVKNAMRIPVLANGNVRCIEDVDNCIKETGVEGVLSAETLLENPA 314

Query: 319 LFAGFRTADWI-EGCEENTRDGNLDQADLLVDYLKLCEKYPVPWRMIRSHVHKLLGDWFK 378
            FAGFRTA+W  +  EE   DG LDQ DL+V+YLKLCEK+PVPWRMIRSHVHK+LG+WF+
Sbjct: 315 AFAGFRTAEWAKDNEEEGFVDGGLDQGDLVVEYLKLCEKHPVPWRMIRSHVHKMLGEWFR 374

Query: 379 IHPHIREDFNAQSKLTFEFLYNMVERLRELQVKIPLYIK 414
           IHP +RE  NAQ+ LTFEFLY +V++LREL  ++PLY K
Sbjct: 375 IHPQVREQLNAQNILTFEFLYGLVDQLRELGGRVPLYKK 408

BLAST of Lag0008809 vs. TAIR 10
Match: AT3G49640.1 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 129.8 bits (325), Expect = 5.2e-30
Identity = 80/247 (32.39%), Postives = 121/247 (48.99%), Query Frame = 0

Query: 95  KLIVAPMVDNSELPFRMLCRKYGAEAAYTPMLHSRIFTENEKYRNME------------- 154
           KL++APMV    L FRML  +YGA+  Y   +      + E+  N+              
Sbjct: 6   KLVLAPMVRVGTLSFRMLAAEYGADITYGEEIIDHKLVKCERRLNVASGTSEFVEKGTDN 65

Query: 155 --FTTCQEDRPLFV-QFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFLM 214
             F+TC E++   V Q   +D    L+A+  V      +D+N+GCP+  + +G  GA L+
Sbjct: 66  VVFSTCDEEKSRVVFQMGTSDAVRALKASEIVCNDVATIDINMGCPKAFSIQGGMGAALL 125

Query: 215 DNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKD 274
               L+  I+  L  NL VPV+CKIR+  +  DT+  AR +E  G   LAVHG  R   D
Sbjct: 126 SKPELIHDILATLKRNLDVPVTCKIRLLKSPADTVELARRIEKLGVPALAVHG--RKIAD 185

Query: 275 GKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVKNCLQETGVEGVLSAETLLENPALFA 326
             +  A W  I+ V  A+ IPV+ANG++   +D       TG   V+ A   + N ++F+
Sbjct: 186 RPRDPAKWDEIADVVAALSIPVIANGDVLEYDDFSRIKTATGAASVMVARGAMWNASIFS 245

BLAST of Lag0008809 vs. TAIR 10
Match: AT3G49640.2 (Aldolase-type TIM barrel family protein )

HSP 1 Score: 111.7 bits (278), Expect = 1.5e-24
Identity = 64/188 (34.04%), Postives = 98/188 (52.13%), Query Frame = 0

Query: 139 NMEFTTCQEDRPLFV-QFCANDPDVLLEAARRVEPYCDYVDLNLGCPQRIARRGNYGAFL 198
           N+ F+TC E++   V Q   +D    L+A+  V      +D+N+GCP+  + +G  GA L
Sbjct: 26  NVVFSTCDEEKSRVVFQMGTSDAVRALKASEIVCNDVATIDINMGCPKAFSIQGGMGAAL 85

Query: 199 MDNLPLVKAIVEKLASNLQVPVSCKIRIFPNLQDTINYARMLEDAGCSLLAVHGRTRDEK 258
           +    L+  I+  L  NL VPV+CKIR+  +  DT+  AR +E  G   LAVHG  R   
Sbjct: 86  LSKPELIHDILATLKRNLDVPVTCKIRLLKSPADTVELARRIEKLGVPALAVHG--RKIA 145

Query: 259 DGKKFRANWSAISAVKNAVRIPVLANGNIRHMEDVKNCLQETGVEGVLSAETLLENPALF 318
           D  +  A W  I+ V  A+ IPV+ANG++   +D       TG   V+ A   + N ++F
Sbjct: 146 DRPRDPAKWDEIADVVAALSIPVIANGDVLEYDDFSRIKTATGAASVMVARGAMWNASIF 205

Query: 319 AGFRTADW 326
           +    + W
Sbjct: 206 SPKGKSHW 211

BLAST of Lag0008809 vs. TAIR 10
Match: AT4G38890.1 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 82.0 bits (201), Expect = 1.2e-15
Identity = 52/200 (26.00%), Postives = 93/200 (46.50%), Query Frame = 0

Query: 93  RPKLIVAPMVDNSELPFRMLCRKYGAEAAYTPMLHSRIFTENEKYRNMEFTTCQEDRPLF 152
           R KL +AP+     LPFR LC+  GA+     M       + +            +    
Sbjct: 337 RDKLYLAPLTTVGNLPFRRLCKVLGADVTCGEMAMCTNLLQGQASEWALLRRHSSEDLFG 396

Query: 153 VQFCANDPDVLLEAARRVEPYC--DYVDLNLGCPQRIARRGNYGAFLMDNLPLVKAIVEK 212
           VQ C + PD +      ++  C  D++D+N+GCP  +    + G+ L++    +K IVE 
Sbjct: 397 VQICGSYPDTVSRVVELIDRECTVDFIDINMGCPIDMVVNKSAGSALLNKPLRMKNIVEV 456

Query: 213 LASNLQVPVSCKIR--IFPNLQDTINYARMLEDAGCSLLAVHGRTRDEKDGKKFRANWSA 272
            +S ++ P++ K+R   F       +    + + G + + +HGR+R ++  K   A+W  
Sbjct: 457 SSSIVETPITIKVRTAFFEGKNRIDSLIADIGNWGATAVTIHGRSRQQRYSKS--ADWDY 516

Query: 273 I-SAVKNA-VRIPVLANGNI 287
           I    KNA   + V+ NG++
Sbjct: 517 IYQCTKNATTNLQVIGNGDV 534

BLAST of Lag0008809 vs. TAIR 10
Match: AT3G63510.1 (FMN-linked oxidoreductases superfamily protein )

HSP 1 Score: 62.4 bits (150), Expect = 1.0e-09
Identity = 49/179 (27.37%), Postives = 81/179 (45.25%), Query Frame = 0

Query: 50  QQQEECSPLTKSDDSSLLLGSPSGYLNGESRIERAWAHWSKLGRPKLIVAPMVDNSELPF 109
           Q Q  C P  + + S +   SP   L   +R     + +     P   VAPM+D ++  +
Sbjct: 13  QFQMWCLPSVRPNVSEMFFKSP--MLKPTARFSSTSSPYLP---PSFSVAPMMDWTDNHY 72

Query: 110 RMLCRKYGAEA-AYTPMLHSRIFTENEKYRNMEFTTCQEDRPLFVQFCANDPDVLLEAAR 169
           R L R     A  YT M+ +      +   +       +  P+ +Q   ++ + L +AA+
Sbjct: 73  RTLARLITKHAWLYTEMIAAETLVHQQTNLDRFLAFSPQQHPIVLQLGGSNVENLAKAAK 132

Query: 170 RVEPY-CDYVDLNLGCPQ-RIARRGNYGAFLMDNLPLVKAIVEKLASNLQVPVSCKIRI 226
             + Y  D ++LN GCP  ++A  G +G  LM    LV   +  +A+N  VPV+ K RI
Sbjct: 133 LSDAYGYDEINLNCGCPSPKVAGHGCFGVSLMLKPKLVGEAMSAIAANTNVPVTVKCRI 186

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038901742.11.9e-22892.67tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Benincasa hispida][more]
XP_022944464.13.3e-22892.45tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Cucurbita moschata][more]
KAG7011024.15.6e-22892.45tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like protein [Cucurbita argyrosp... [more]
XP_022986543.11.6e-22792.22tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Cucurbita maxima][more]
XP_023512932.11.4e-22691.98tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like [Cucurbita pepo subsp. pepo... [more]
Match NameE-valueIdentityDescription
Q8K5824.5e-7947.65tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Rattus norvegicus OX=101... [more]
Q8C2P37.7e-7947.32tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Mus musculus OX=10090 GN... [more]
Q6P1R42.9e-7847.32tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Homo sapiens OX=9606 GN=... [more]
Q9HGN65.5e-6945.36tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)] OS=Schizosaccharomyces pombe (st... [more]
P537591.4e-6441.27tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)] OS=Saccharomyces cerevisiae (str... [more]
Match NameE-valueIdentityDescription
A0A6J1FWR01.6e-22892.45tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucurbita moschata OX=36... [more]
A0A6J1JGU37.9e-22892.22tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucurbita maxima OX=3661... [more]
A0A1S3CHU61.1e-22691.53tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucumis melo OX=3656 GN=... [more]
A0A6J1D4R63.3e-22689.39tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Momordica charantia OX=3... [more]
A0A6J1G6725.9e-22390.59tRNA-dihydrouridine(16/17) synthase [NAD(P)(+)]-like OS=Cucurbita moschata OX=36... [more]
Match NameE-valueIdentityDescription
AT5G67220.11.0e-17473.68FMN-linked oxidoreductases superfamily protein [more]
AT3G49640.15.2e-3032.39Aldolase-type TIM barrel family protein [more]
AT3G49640.21.5e-2434.04Aldolase-type TIM barrel family protein [more]
AT4G38890.11.2e-1526.00FMN-linked oxidoreductases superfamily protein [more]
AT3G63510.11.0e-0927.37FMN-linked oxidoreductases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013785Aldolase-type TIM barrelGENE3D3.20.20.70Aldolase class Icoord: 93..325
e-value: 3.6E-80
score: 270.6
IPR035587DUS-like, FMN-binding domainPFAMPF01207Duscoord: 97..388
e-value: 5.9E-74
score: 249.0
IPR035587DUS-like, FMN-binding domainCDDcd02801DUS_like_FMNcoord: 95..321
e-value: 8.87665E-107
score: 313.277
NoneNo IPR availablePANTHERPTHR11082TRNA-DIHYDROURIDINE SYNTHASEcoord: 76..413
NoneNo IPR availablePANTHERPTHR11082:SF35BNAA09G07510D PROTEINcoord: 76..413
NoneNo IPR availableSUPERFAMILY51395FMN-linked oxidoreductasescoord: 94..370
IPR018517tRNA-dihydrouridine synthase, conserved sitePROSITEPS01136UPF0034coord: 177..195

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0008809.1Lag0008809.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0002943 tRNA dihydrouridine synthesis
biological_process GO:0008033 tRNA processing
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0017150 tRNA dihydrouridine synthase activity
molecular_function GO:0003824 catalytic activity