Lag0008665 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0008665
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionATP-dependent (S)-NAD(P)H-hydrate dehydratase
Locationchr9: 27562273 .. 27566104 (+)
RNA-Seq ExpressionLag0008665
SyntenyLag0008665
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTTGGCTTCATCGGCTGTGTTTAGACGACAACAGTTCTTGATAAGGTCTCTTGGAGGTTTTGGTGATTGTACTCATCAGAATAGGCAGCAACAAGTCAAAGCCATGAGTGGGGCTTCTATTGAGGCTGATGCTGAAAACATTCTAAGAGCAATTACACCTAGTCTTGATCCCAACAGATACAAAGGCCAGGCGGGTAAGCTCTAAAGATACTGTGTACTTCTCTGGATTTTCTTTTATATTCTATTTGCAATGCACAATTGGTTGAGAACTAATCAAGTTTTTTGTTGTCATGAATTAGTTTGCAATAACTTTATGGTAAGTCAAAAATTCAAAATAGTGGTTTTGGCTTCTTGGTAGATTTTGCTAACTCAATTTGAATATTGCTCTTTGATGCTAGTTTCCATTTGTCTTCCAATTGTAATTTGTGGTACTTTGTGCTCTATCCAGTGTCGTCCATTGGTTTGGTTCTCAGAAGTCCTCTAACTAACAATCTATATCTATATGTCAGGGAAGATAGCTGTTATTGGTGGATGTCGTGAGTACACAGGTGCTCCCTACTTCGCTGCTATATCAGCACTGAAAATTGTTAGTTCAGACTCTTTTTCTTGGTTGTCTGGGTAGAGAGTTTTATTCTATGTTTTCGATTCAAATATTTCATTTTCCTCTTTTTACTTGCATAGGTGAATAACAGCCTATTAGTTTTTCCTTCCAGGGTGCAGATTTATCTCATGTTTTTTGCACAAAAGATGCTGCTCCTGTCATAAAGAGCTATAGTCCAGAGTTAATTGTGCATCCTATTTTGGAAGAATCGTACAGTGTCAGGTGCTTCTGCTTTCTATATTTTGGTCTGAGTAAAAAAAGTTCAACAGAAATTTTGATATTTCTCTGGTGCGTTCTGATGTTTTAGAGATGAGGAAAAGAAGTTAATCGCAGAGAGGGTTCTTGCTGAGGTTGACAAGTGGCTGGAAAGATTTGATTGTCTTGTCATTGGTCCAGGCCTTGGAAGGGATCCATTTCTTCTGGTGATTGTTATTGCTTTTTGGCCCAATTACATAGTTCCTTCTTGAAATATACACTTGATTTCAATTTTGTCCTTGGAAGTTTTAAAATTTCATCCTTACTTCCAAACTTTCAAACTTTGGTTGACATGATTGATAAAATACGATTTTTCTATAGATTTGGCAAGTGGAATATAGAAAATACCTTGTCCTTTTTTCTTTCTTCGTGCTAACTGCTGAGTGAAAGAAGGCTAGTTCACTTGCCAAATCAAGCCTCATTTTGTTAAGCACGTGAATCAGTTGTTTTAAAGATTTGATTCTGAGTCTCTTAACAAAAAACGGAAGAAATTTGATTTCTGAGTCTCTTTTTTGCCTCTGCAGTCCACTAAAATGGTGAGATGAAGTTAATTCATCAGTTATTGCTTTATCTACGTTGATCTGATAATTTTTTGTAGTTGCTTTGGCTGTTAGCCTATGCCTATGTATTGTTTCAACAAGTTGTCAAACATGAATTATACAGCTTCTCTCAAGGTTGAAAGTGATATTTAAGATCTAACTACCTTCTCCTTGCTTTAGGCTTTCATAATTTCCTTCCCATTTGTCTTACATGTTTCAATCGGGCGTCAAATTTTATGCATGTTTCTTTCTCTTGCCTATGCCATAAAATTTTCTAATGGTTGAAACTGTTCCAGGATTGTGTCAGTGAAATCATAAAGCATGCAAGGCAGACCAATATAACAATGGTCATAGACGGGGTAACCTAACTTGAAAGAAATTGCCTTGTTTATATTATTGTTATCCATGCCATTTTCAACAGGTCTAATAAGTGGCTGCTTACTGCTGATTGTGTTTGTCGCTTTTTTAGGATGGTCTGTTTCTTATTACTGACAATCTTCACCTTGTAAGCAATTACCCCCTGGCAGTCCTGACTCCAAATGTAAATGAGTATAAACGTCTTGTGAAAAAGGTGTTGCTTGTTGAAGTTGATGAACAAGATGCTCCTAACCAATTGCTGTCTCTTGCCAAACGGTAAAGCCTTCATGTTTTTCATTTGAATAGAGTTTCCATATGTCATGATTATGCAATGGTGACATGCTTATTACTCAAGTGAATCTTGTATGGAGATGCTACTTACCACGACCCTGAAATCAGCATATTAGTGATTGCATAATTAAGAAGTTTCTTATTAGTTATTAAGGTACATGTATTTGCAAAAGTTGGCAGATGAAAGGTTGACCTCATCTGATCAGAATTCAGAGTAGATGTTACTACCTATTCAGGGCTCATAACTCTATTGGCACATTCTGAAGTCTCTATCCAAATGGTTCACAATGTCAGGAACGGGGATATTTTGGTTGTGTGCCCAATCGAATAGTCTAATCACTCTTTTGATTCATTGCATACAACTTTTTTGACATATTTGTTTCTGAAGTATGAAGGTCATAACATTAGGATGAAAACTGTAACTTTATCTTATAGAAGGAATTGTTGTGTTGGCTAGCTATAAATAATCATGCCGCAGATATCTTATTGCTCAGTTTAGATGGAGATAGCTTTTCTTATGTGGATTTATGCTTCATTCTATTTTATAATTTCTAACAAATATCATTAAGTGTTATTTCATTCATTTTTCCATTCTTTGGCTCACTAAACTTTTGGACATGATCAGGATTGGTGGCCTAACTATCCTCAGGAAAGGGAGAGCTGACCTCATTAGTGACGGTGAGACAGGTACTGATCACAACTACAAAGTATTAACCATTTTAATGTTATAAAAGTGCTACAAATTCAATGTTCCTTTTCAATGATTTCTAAGTCGTTGTGTCACCACCACCAACCCTTAAGTTCCGCTGTAGCTTACCTTTTGGTCAGATTAGGAAAACAAAAACTATACAATTCTATGTTATGTATGGCTAAGCTGTTATCCTGCCGCCATGGAGGAAGTGAGCCAAATTGAATATGGATTAAAATGTTCTTTCGAATTTCTGAAATTTTCACAGGATTTCTGGTTTGGATCTGTCATAATGTCCATGAAAATTTAGGAATTCGGATTTCTAAGTGTTGTAAATATTAATATGGTGTTCTCTGTTGCTATTGCTAGATTATACTTGTTTCTTCCATTAACTGAAATCATGTGACACCTGATGCAGTCAAATCTGTGAGCATTTATGGTTCTCCTCGCCGTTGTGGTGGCCAAGGTGATATACTTTCAGGAAGGCAAGTATTGCCATGCGTTTTCATTATTCTTGGTGAATATGGTTCTCTCTCTTAAACTGAATATATATATATGTACCTTATTTCATACTCCAAGAGCCAAGAAATCGAAAGTTCATGAATGTCTTTACAATAAGGTCACAAGTCTTTTCTTTCCTCCTGTGGTCCAGTATTGCAGTTTTTGTATCTTGGGCTCGTCGGCAAGGTTTAGTCACAGATGGGAATTTGGCTACCAGGCAAGTTCCAAAAGTTAACCATCATCTGGTCTTTTGATCTCATTTGACCAATACTGATTCCCTATGTTGTCTTTCATGAAACCCTTTTCACATGTAGCCCAAAGAATCCAATAGTGTTAGGGTGTGTTGCTGGATCTGGTTTACTTAGAAGGGCTGCATCACTTGCATTCGAGAATAAGAAAAGATCAACCCTTACTACTGATATCATCGAGTTCTTGTGGAGAAGGTACTGTCAGCTTTAAGAATGGAGATTATTTCAATATAAAAACTAAATCAGCTTCTAATATTGGTTCATTCCTTTCGCAGTCTGGAGGATATTTCTCCAGCCCAATAATCGAATCTGTTGGTCTTGCAACTGTGCTGAAGATACTTGTGACATGA

mRNA sequence

ATGTTGGCTTCATCGGCTGTGTTTAGACGACAACAGTTCTTGATAAGGTCTCTTGGAGGTTTTGGTGATTGTACTCATCAGAATAGGCAGCAACAAGTCAAAGCCATGAGTGGGGCTTCTATTGAGGCTGATGCTGAAAACATTCTAAGAGCAATTACACCTAGTCTTGATCCCAACAGATACAAAGGCCAGGCGGGGAAGATAGCTGTTATTGGTGGATGTCGTGAGTACACAGGTGCTCCCTACTTCGCTGCTATATCAGCACTGAAAATTGGTGCAGATTTATCTCATGTTTTTTGCACAAAAGATGCTGCTCCTGTCATAAAGAGCTATAGTCCAGAGTTAATTGTGCATCCTATTTTGGAAGAATCGTACAGTGTCAGAGATGAGGAAAAGAAGTTAATCGCAGAGAGGGTTCTTGCTGAGGTTGACAAGTGGCTGGAAAGATTTGATTGTCTTGTCATTGGTCCAGGCCTTGGAAGGGATCCATTTCTTCTGGATTGTGTCAGTGAAATCATAAAGCATGCAAGGCAGACCAATATAACAATGGTCATAGACGGGGATGGTCTGTTTCTTATTACTGACAATCTTCACCTTGTAAGCAATTACCCCCTGGCAGTCCTGACTCCAAATGTAAATGAGTATAAACGTCTTGTGAAAAAGGTGTTGCTTGTTGAAGTTGATGAACAAGATGCTCCTAACCAATTGCTGTCTCTTGCCAAACGGATTGGTGGCCTAACTATCCTCAGGAAAGGGAGAGCTGACCTCATTAGTGACGGTGAGACAGTCAAATCTGTGAGCATTTATGGTTCTCCTCGCCGTTGTGGTGGCCAAGGTGATATACTTTCAGGAAGGCAAGTATTGCCATGCGTTTTCATTATTCTTGGTGAATATGTTTTTGTATCTTGGGCTCGTCGGCAAGGTTTAGTCACAGATGGGAATTTGGCTACCAGGCAAGTTCCAAAAGTTAACCATCATCTGTCTGGAGGATATTTCTCCAGCCCAATAATCGAATCTGTTGGTCTTGCAACTGTGCTGAAGATACTTGTGACATGA

Coding sequence (CDS)

ATGTTGGCTTCATCGGCTGTGTTTAGACGACAACAGTTCTTGATAAGGTCTCTTGGAGGTTTTGGTGATTGTACTCATCAGAATAGGCAGCAACAAGTCAAAGCCATGAGTGGGGCTTCTATTGAGGCTGATGCTGAAAACATTCTAAGAGCAATTACACCTAGTCTTGATCCCAACAGATACAAAGGCCAGGCGGGGAAGATAGCTGTTATTGGTGGATGTCGTGAGTACACAGGTGCTCCCTACTTCGCTGCTATATCAGCACTGAAAATTGGTGCAGATTTATCTCATGTTTTTTGCACAAAAGATGCTGCTCCTGTCATAAAGAGCTATAGTCCAGAGTTAATTGTGCATCCTATTTTGGAAGAATCGTACAGTGTCAGAGATGAGGAAAAGAAGTTAATCGCAGAGAGGGTTCTTGCTGAGGTTGACAAGTGGCTGGAAAGATTTGATTGTCTTGTCATTGGTCCAGGCCTTGGAAGGGATCCATTTCTTCTGGATTGTGTCAGTGAAATCATAAAGCATGCAAGGCAGACCAATATAACAATGGTCATAGACGGGGATGGTCTGTTTCTTATTACTGACAATCTTCACCTTGTAAGCAATTACCCCCTGGCAGTCCTGACTCCAAATGTAAATGAGTATAAACGTCTTGTGAAAAAGGTGTTGCTTGTTGAAGTTGATGAACAAGATGCTCCTAACCAATTGCTGTCTCTTGCCAAACGGATTGGTGGCCTAACTATCCTCAGGAAAGGGAGAGCTGACCTCATTAGTGACGGTGAGACAGTCAAATCTGTGAGCATTTATGGTTCTCCTCGCCGTTGTGGTGGCCAAGGTGATATACTTTCAGGAAGGCAAGTATTGCCATGCGTTTTCATTATTCTTGGTGAATATGTTTTTGTATCTTGGGCTCGTCGGCAAGGTTTAGTCACAGATGGGAATTTGGCTACCAGGCAAGTTCCAAAAGTTAACCATCATCTGTCTGGAGGATATTTCTCCAGCCCAATAATCGAATCTGTTGGTCTTGCAACTGTGCTGAAGATACTTGTGACATGA

Protein sequence

MLASSAVFRRQQFLIRSLGGFGDCTHQNRQQQVKAMSGASIEADAENILRAITPSLDPNRYKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPILEESYSVRDEEKKLIAERVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHARQTNITMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKKVLLVEVDEQDAPNQLLSLAKRIGGLTILRKGRADLISDGETVKSVSIYGSPRRCGGQGDILSGRQVLPCVFIILGEYVFVSWARRQGLVTDGNLATRQVPKVNHHLSGGYFSSPIIESVGLATVLKILVT
Homology
BLAST of Lag0008665 vs. NCBI nr
Match: XP_023528365.1 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase [Cucurbita pepo subsp. pepo])

HSP 1 Score: 567.0 bits (1460), Expect = 1.1e-157
Identity = 287/317 (90.54%), Postives = 294/317 (92.74%), Query Frame = 0

Query: 1   MLASSAVFRRQQFLIRSLGGFGDCTHQNRQQQVKAMSGASIEADAENILRAITPSLDPNR 60
           MLASSAVFRRQQFLIRSLGGFGDCTHQNR+QQ+KAMSG S+EADAENILRAITPSLDP R
Sbjct: 1   MLASSAVFRRQQFLIRSLGGFGDCTHQNRRQQIKAMSGTSMEADAENILRAITPSLDPKR 60

Query: 61  YKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPI 120
           YKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHP+
Sbjct: 61  YKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPV 120

Query: 121 LEESYSVRDEEKKLIAERVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHARQTN 180
           LEESYSVRDEEKK  AERVLAEVDKWLERFDCLVIGPGLGRDPFLL+CVSEIIKHARQTN
Sbjct: 121 LEESYSVRDEEKKFTAERVLAEVDKWLERFDCLVIGPGLGRDPFLLECVSEIIKHARQTN 180

Query: 181 ITMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKKVLLVEVDEQDAPNQLLSLA 240
           I M+IDGDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVK VLL EVDEQDAPNQLLSL 
Sbjct: 181 IPMIIDGDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKNVLLAEVDEQDAPNQLLSLT 240

Query: 241 KRIGGLTILRKGRADLISDGETVKSVSIYGSPRRCGGQGDILSGRQVLPCVFIILGEYVF 300
           KRIGGLTILRKGRADLI DGETVKSVSIYGSPRRCGGQGDILSG              VF
Sbjct: 241 KRIGGLTILRKGRADLICDGETVKSVSIYGSPRRCGGQGDILSGSVA-----------VF 300

Query: 301 VSWARRQGLVTDGNLAT 318
           VSWAR+QGLVTDGNLAT
Sbjct: 301 VSWARQQGLVTDGNLAT 306

BLAST of Lag0008665 vs. NCBI nr
Match: XP_022955699.1 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase [Cucurbita moschata])

HSP 1 Score: 566.6 bits (1459), Expect = 1.5e-157
Identity = 287/317 (90.54%), Postives = 294/317 (92.74%), Query Frame = 0

Query: 1   MLASSAVFRRQQFLIRSLGGFGDCTHQNRQQQVKAMSGASIEADAENILRAITPSLDPNR 60
           MLASSAVFRRQQFLIRSLGGFGDC HQNR+QQ+KAMSG S+EADAENILRAITPSLDP R
Sbjct: 1   MLASSAVFRRQQFLIRSLGGFGDCAHQNRRQQIKAMSGTSMEADAENILRAITPSLDPKR 60

Query: 61  YKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPI 120
           YKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHP+
Sbjct: 61  YKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPV 120

Query: 121 LEESYSVRDEEKKLIAERVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHARQTN 180
           LEESYSVRDEEKK  AERVLAEVDKWLERFDCLVIGPGLGRDPFLL+CVSEIIKHARQTN
Sbjct: 121 LEESYSVRDEEKKFTAERVLAEVDKWLERFDCLVIGPGLGRDPFLLECVSEIIKHARQTN 180

Query: 181 ITMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKKVLLVEVDEQDAPNQLLSLA 240
           I M+IDGDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVK VLL EVDEQDAPNQLLSLA
Sbjct: 181 IPMIIDGDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKNVLLAEVDEQDAPNQLLSLA 240

Query: 241 KRIGGLTILRKGRADLISDGETVKSVSIYGSPRRCGGQGDILSGRQVLPCVFIILGEYVF 300
           KRIGGLTILRKGRADLI DGETVKSVSIYGSPRRCGGQGDILSG              VF
Sbjct: 241 KRIGGLTILRKGRADLICDGETVKSVSIYGSPRRCGGQGDILSGSVA-----------VF 300

Query: 301 VSWARRQGLVTDGNLAT 318
           VSWAR+QGLVTDGNLAT
Sbjct: 301 VSWARQQGLVTDGNLAT 306

BLAST of Lag0008665 vs. NCBI nr
Match: XP_038878236.1 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase isoform X1 [Benincasa hispida])

HSP 1 Score: 565.1 bits (1455), Expect = 4.3e-157
Identity = 287/317 (90.54%), Postives = 294/317 (92.74%), Query Frame = 0

Query: 1   MLASSAVFRRQQFLIRSLGGFGDCTHQNRQQQVKAMSGASIEADAENILRAITPSLDPNR 60
           MLASSAVFRRQQFLIRSLGGFGDCT+QN++QQ+KAMSG  IEADA NILRAITPSLDPNR
Sbjct: 1   MLASSAVFRRQQFLIRSLGGFGDCTYQNKRQQIKAMSGGYIEADAANILRAITPSLDPNR 60

Query: 61  YKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPI 120
           YKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHP+
Sbjct: 61  YKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPV 120

Query: 121 LEESYSVRDEEKKLIAERVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHARQTN 180
           LEESYSVRDEEKK IAERVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEII+HARQ N
Sbjct: 121 LEESYSVRDEEKKFIAERVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIRHARQNN 180

Query: 181 ITMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKKVLLVEVDEQDAPNQLLSLA 240
           I MVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVK VLL EVDEQDAPNQLLSLA
Sbjct: 181 IPMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKNVLLAEVDEQDAPNQLLSLA 240

Query: 241 KRIGGLTILRKGRADLISDGETVKSVSIYGSPRRCGGQGDILSGRQVLPCVFIILGEYVF 300
           KRIGGLTILRKGRADLISDGETVKSVSIYGSPRRCGGQGDILSG              VF
Sbjct: 241 KRIGGLTILRKGRADLISDGETVKSVSIYGSPRRCGGQGDILSGSVA-----------VF 300

Query: 301 VSWARRQGLVTDGNLAT 318
           VSWA+RQG VTDGNLAT
Sbjct: 301 VSWAQRQGFVTDGNLAT 306

BLAST of Lag0008665 vs. NCBI nr
Match: XP_022979515.1 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase [Cucurbita maxima])

HSP 1 Score: 564.7 bits (1454), Expect = 5.6e-157
Identity = 287/317 (90.54%), Postives = 294/317 (92.74%), Query Frame = 0

Query: 1   MLASSAVFRRQQFLIRSLGGFGDCTHQNRQQQVKAMSGASIEADAENILRAITPSLDPNR 60
           MLASSAVFRRQQFLIRSLGGFGDCTHQNR+QQ+KAMSG S+EADAENILRAITPSLDP R
Sbjct: 1   MLASSAVFRRQQFLIRSLGGFGDCTHQNRRQQIKAMSGTSMEADAENILRAITPSLDPKR 60

Query: 61  YKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPI 120
           +KGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHP+
Sbjct: 61  HKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPV 120

Query: 121 LEESYSVRDEEKKLIAERVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHARQTN 180
           LEESYSVRDEEKK  AERVLAEVDKWLERFDCLVIGPGLGRDPFLL+CVSEIIKHARQTN
Sbjct: 121 LEESYSVRDEEKKFTAERVLAEVDKWLERFDCLVIGPGLGRDPFLLECVSEIIKHARQTN 180

Query: 181 ITMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKKVLLVEVDEQDAPNQLLSLA 240
           I MVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVK VLL EVDEQDAPNQLLSLA
Sbjct: 181 IPMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKNVLLAEVDEQDAPNQLLSLA 240

Query: 241 KRIGGLTILRKGRADLISDGETVKSVSIYGSPRRCGGQGDILSGRQVLPCVFIILGEYVF 300
           KRIGGLTILRKGRADLI DGETVKSVSIYGSPRRCGGQGDILSG              VF
Sbjct: 241 KRIGGLTILRKGRADLICDGETVKSVSIYGSPRRCGGQGDILSGSVA-----------VF 300

Query: 301 VSWARRQGLVTDGNLAT 318
           VSWAR+QGLV DGNLAT
Sbjct: 301 VSWARQQGLVIDGNLAT 306

BLAST of Lag0008665 vs. NCBI nr
Match: KAG7018682.1 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 564.3 bits (1453), Expect = 7.3e-157
Identity = 292/346 (84.39%), Postives = 303/346 (87.57%), Query Frame = 0

Query: 1   MLASSAVFRRQQFLIRSLGGFGDCTHQNRQQQVKAMSGASIEADAENILRAITPSLDPNR 60
           MLASSAVFRRQQFLIRSLGGFGDCTHQNR+QQ+KAMSG S+EADAENILRAITPSLDP R
Sbjct: 1   MLASSAVFRRQQFLIRSLGGFGDCTHQNRRQQIKAMSGTSMEADAENILRAITPSLDPKR 60

Query: 61  YKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPI 120
           YKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHP+
Sbjct: 61  YKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPV 120

Query: 121 LEESYSVRDEEKKLIAERVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHARQTN 180
           LEESYSVRDEEKK  AERVLAEVDKWLERFDCLVIGPGLGRDPFLL+CVSEIIKHARQTN
Sbjct: 121 LEESYSVRDEEKKFTAERVLAEVDKWLERFDCLVIGPGLGRDPFLLECVSEIIKHARQTN 180

Query: 181 ITMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKKVLLVEVDEQDAPNQLLSLA 240
           I M+IDGDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVK VLL EVDEQDAPNQLLSLA
Sbjct: 181 IPMIIDGDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKNVLLAEVDEQDAPNQLLSLA 240

Query: 241 KRIGGLTILRKGRADLISDGETVKSVSIYGSPRRCGGQGDILSGRQVLPCVFIILGEYVF 300
           KRIGGLTILRKGRADLI DGETVKSVSIYGSPRRCGGQ                    VF
Sbjct: 241 KRIGGLTILRKGRADLICDGETVKSVSIYGSPRRCGGQ--------------------VF 300

Query: 301 VSWARRQGLVTDGNLATRQVPKVNHHLSGGYFSSPIIESVGLATVL 347
           VSWAR+QGLVTDGNLATRQV +  H L   +F S +  +    TVL
Sbjct: 301 VSWARQQGLVTDGNLATRQVLEFKHRL---FFRSHLTNTPKNPTVL 323

BLAST of Lag0008665 vs. ExPASy Swiss-Prot
Match: F6HDM2 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Vitis vinifera OX=29760 GN=VIT_05s0020g02800 PE=3 SV=1)

HSP 1 Score: 448.0 bits (1151), Expect = 1.0e-124
Identity = 233/315 (73.97%), Postives = 265/315 (84.13%), Query Frame = 0

Query: 1   MLASSAVFRRQQFLIRSLGGFGDCTHQNRQQQVKAMSGASIEADAENILRAITPSLDPNR 60
           MLASSAVFRRQ+FLIR LG  G      R+   + M   ++EADAENILRAITP+LD  R
Sbjct: 1   MLASSAVFRRQEFLIRCLGVGGQSQQFYRKSIPRTM---ALEADAENILRAITPTLDLAR 60

Query: 61  YKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPI 120
           +KGQAGKIAVIGGCREYTGAPYF+AISALKIGADLSHVFCTKDAAPVIKSYSPELIVHP+
Sbjct: 61  HKGQAGKIAVIGGCREYTGAPYFSAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPL 120

Query: 121 LEESYSVRDEEKKLIAERVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHARQTN 180
           LEESYSVR+E+KK I+E+VL EV KW+ERFDCLV+GPGLGRDPFLL CVSEI+KHARQ+N
Sbjct: 121 LEESYSVREEDKKAISEKVLTEVVKWMERFDCLVVGPGLGRDPFLLGCVSEIMKHARQSN 180

Query: 181 ITMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKKVLLVEVDEQDAPNQLLSLA 240
           + +VIDGDGLFL+T++L LVS YPLAVLTPNVNEYKRLV+KVL  EV +QDA  QLLSLA
Sbjct: 181 VPIVIDGDGLFLVTNSLDLVSGYPLAVLTPNVNEYKRLVQKVLNCEVGDQDAAEQLLSLA 240

Query: 241 KRIGGLTILRKGRADLISDGETVKSVSIYGSPRRCGGQGDILSGRQVLPCVFIILGEYVF 300
           K IGG+TILRKG++DLISDGETV SV IYGSPRRCGGQGDILSG              VF
Sbjct: 241 KGIGGVTILRKGKSDLISDGETVNSVGIYGSPRRCGGQGDILSGSVA-----------VF 300

Query: 301 VSWARRQGLVTDGNL 316
           +SWA RQ ++ +G+L
Sbjct: 301 LSWA-RQRIIAEGDL 300

BLAST of Lag0008665 vs. ExPASy Swiss-Prot
Match: Q94AF2 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Arabidopsis thaliana OX=3702 GN=At5g19150 PE=1 SV=1)

HSP 1 Score: 410.2 bits (1053), Expect = 2.3e-113
Identity = 213/307 (69.38%), Postives = 255/307 (83.06%), Query Frame = 0

Query: 3   ASSAVFRRQQFLIRSLGGFGDCTHQNRQQQVKAMSGASIEADAENILRAITPSLDPNRYK 62
           +SS+V RRQ+FL+R+L G            ++AMS  S EADAE++LR +TPSLD  R+K
Sbjct: 21  SSSSVLRRQEFLVRTLCG---------SPIIRAMSSTS-EADAESVLRTVTPSLDLKRHK 80

Query: 63  GQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPILE 122
           GQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHP+LE
Sbjct: 81  GQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPVLE 140

Query: 123 ESYSV---RDEEKKLIAERVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHARQT 182
           ESYS+    +E+K+ + ++VL EV KW+ERFDCLVIGPGLGRDPFLL+CVS I+  A+++
Sbjct: 141 ESYSISQLSEEDKREVQDKVLGEVGKWMERFDCLVIGPGLGRDPFLLECVSIIMLLAKKS 200

Query: 183 NITMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKKVLLVEVDEQDAPNQLLSL 242
           N+  VIDGDGLFL+T+++ LV +YPLAVLTPNVNEYKRLV+KVL  EVDEQ+A +QL SL
Sbjct: 201 NVPFVIDGDGLFLVTNSIDLVHSYPLAVLTPNVNEYKRLVQKVLNCEVDEQNAEDQLRSL 260

Query: 243 AKRIGGLTILRKGRADLISDGETVKSVSIYGSPRRCGGQGDILSGRQVLPCVFIILGEYV 302
           AK+IGG+TILRKG++DLIS+GETVKSVSIYGSPRRCGGQGDILSG           G  V
Sbjct: 261 AKQIGGVTILRKGKSDLISNGETVKSVSIYGSPRRCGGQGDILSG-----------GVAV 306

Query: 303 FVSWARR 307
           F+SWA++
Sbjct: 321 FLSWAQQ 306

BLAST of Lag0008665 vs. ExPASy Swiss-Prot
Match: C5Y210 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Sorghum bicolor OX=4558 GN=Sb05g010020 PE=3 SV=1)

HSP 1 Score: 385.2 bits (988), Expect = 8.0e-106
Identity = 203/322 (63.04%), Postives = 241/322 (74.84%), Query Frame = 0

Query: 1   MLASSAVFRRQQFLIRSL----------GGF----GDCTHQNRQQQVKAM---SGASIEA 60
           M A+S  FRRQ  L+RSL           GF      C+   R +   AM   +G   EA
Sbjct: 23  MWAASPAFRRQLVLLRSLLPSPPAPSSVAGFPPSCPSCSSFLRVRTNHAMAASAGTVYEA 82

Query: 61  DAENILRAITPSLDPNRYKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKD 120
           DAE ++R ITP LD  R+KGQAGKIAVIGGCREYTGAPYFAAISALK+GADLSHVFCTKD
Sbjct: 83  DAEAVVRRITPPLDRARHKGQAGKIAVIGGCREYTGAPYFAAISALKVGADLSHVFCTKD 142

Query: 121 AAPVIKSYSPELIVHPILEESYSVRDEEKKLIAERVLAEVDKWLERFDCLVIGPGLGRDP 180
           AA VIKSYSPELIVHPILEESYSVRD+E+  ++ ++L EV KW+ERFDC+V+GPGLGRD 
Sbjct: 143 AATVIKSYSPELIVHPILEESYSVRDDERASVSSKILTEVGKWMERFDCIVVGPGLGRDS 202

Query: 181 FLLDCVSEIIKHARQTNITMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKKVL 240
           FLLDCVS I++HARQ NI  V+DGDGLFL+T+NL LV   PLA+LTPNV EYKRLV+KVL
Sbjct: 203 FLLDCVSNIMRHARQANIPTVVDGDGLFLVTNNLSLVEGNPLAILTPNVYEYKRLVQKVL 262

Query: 241 LVEVDEQDAPNQLLSLAKRIGGLTILRKGRADLISDGETVKSVSIYGSPRRCGGQGDILS 300
             +VDE+ A  QL++L ++IG +TI++KG+AD+ISDG+TV  VS +GSPRRCGGQGDILS
Sbjct: 263 NCDVDEETASEQLITLCQKIGDVTIMQKGKADVISDGKTVTQVSTFGSPRRCGGQGDILS 322

Query: 301 GRQVLPCVFIILGEYVFVSWAR 306
           G              VF SWAR
Sbjct: 323 GSVA-----------VFASWAR 333

BLAST of Lag0008665 vs. ExPASy Swiss-Prot
Match: A9RY03 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Physcomitrium patens OX=3218 GN=PHYPADRAFT_121160 PE=3 SV=1)

HSP 1 Score: 323.6 bits (828), Expect = 2.9e-87
Identity = 167/269 (62.08%), Postives = 198/269 (73.61%), Query Frame = 0

Query: 49  LRAITPSLDPNRYKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVI 108
           L  + P L P R+KGQAGKIAVIGGCREYTGAPYFAAISALK+GADL+HVFCT  AA VI
Sbjct: 15  LEKVVPVLAPGRHKGQAGKIAVIGGCREYTGAPYFAAISALKMGADLAHVFCTSGAATVI 74

Query: 109 KSYSPELIVHPILEESYSV---RDEEKKLIAERVLAEVDKWLERFDCLVIGPGLGRDPFL 168
           KSYSPELIVHP+L ESY V    +EE   + ++VLAEV KWL+RFDC+VIGPGLGRDP L
Sbjct: 75  KSYSPELIVHPVLHESYDVGEIGEEEISGLKDKVLAEVGKWLQRFDCIVIGPGLGRDPIL 134

Query: 169 LDCVSEIIKHARQTNITMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKKVL-- 228
           LDCV+ II+ A+  NI +V+DGDGLFL+T+   L+  YPLA+LTPNV E+KRLV K++  
Sbjct: 135 LDCVAAIIEEAKFKNIPLVLDGDGLFLVTNQPELIIGYPLAILTPNVMEHKRLVAKIVGE 194

Query: 229 -------LVEVDEQDAPNQLLSLAKRIGGLTILRKGRADLISDGETVKSVSIYGSPRRCG 288
                    EV  +D P QL  LAKR+ G+TIL+KG+ D ISDG+TV S   YGSPRRCG
Sbjct: 195 RDQNVPQNPEVSNEDLPGQLQDLAKRMEGVTILQKGKTDYISDGKTVLSSDYYGSPRRCG 254

Query: 289 GQGDILSGRQVLPCVFIILGEYVFVSWAR 306
           GQGD+LSG              VFVSWA+
Sbjct: 255 GQGDVLSGSTA-----------VFVSWAK 272

BLAST of Lag0008665 vs. ExPASy Swiss-Prot
Match: E1BNQ4 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Bos taurus OX=9913 GN=NAXD PE=3 SV=1)

HSP 1 Score: 249.2 bits (635), Expect = 6.8e-65
Identity = 137/268 (51.12%), Postives = 176/268 (65.67%), Query Frame = 0

Query: 44  DAENIL---RAITPSLDPNRYKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFC 103
           D E+IL   R++ P+L   ++KGQ G+I V+GGCREYTGAPYFAAISALK+GADLSHVFC
Sbjct: 31  DMESILQLVRSVVPALTTKKHKGQDGRIGVVGGCREYTGAPYFAAISALKVGADLSHVFC 90

Query: 104 TKDAAPVIKSYSPELIVHPILEESYSVRDEEKKLIAERVLAEVDKWLERFDCLVIGPGLG 163
           T++AAPVIK+YSPELIVHP+L+   +VRD             V++WL R   LV+GPGLG
Sbjct: 91  TQEAAPVIKAYSPELIVHPVLDSPEAVRD-------------VEQWLPRLHALVVGPGLG 150

Query: 164 RDPFLLDCVSEIIKHARQTNITMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVK 223
           RD  LL+ V  I++ ++   I +VID DGL+LI     L+  Y  AVLTPN  E+ RL +
Sbjct: 151 RDDALLENVKGILEASKARGIPVVIDADGLWLIAQQPALIQGYRKAVLTPNHVEFGRLSE 210

Query: 224 KVLLVEVDEQDAPNQLLSLAKRIGGLTILRKGRADLISDGETVKSVSIYGSPRRCGGQGD 283
            VL V +D  D    +L L++ +G +T+++KG  D+ISDGE V   S  GS RRCGGQGD
Sbjct: 211 AVLGVPLDGGDRHGAVLRLSQALGNVTVVQKGEQDVISDGEQVLECSQEGSGRRCGGQGD 270

Query: 284 ILSGRQVLPCVFIILGEYVFVSWARRQG 309
           +LSG          LG  V   WA R G
Sbjct: 271 LLSGS---------LG--VLAHWALRAG 274

BLAST of Lag0008665 vs. ExPASy TrEMBL
Match: A0A6J1GX13 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Cucurbita moschata OX=3662 GN=LOC111457620 PE=3 SV=1)

HSP 1 Score: 566.6 bits (1459), Expect = 7.1e-158
Identity = 287/317 (90.54%), Postives = 294/317 (92.74%), Query Frame = 0

Query: 1   MLASSAVFRRQQFLIRSLGGFGDCTHQNRQQQVKAMSGASIEADAENILRAITPSLDPNR 60
           MLASSAVFRRQQFLIRSLGGFGDC HQNR+QQ+KAMSG S+EADAENILRAITPSLDP R
Sbjct: 1   MLASSAVFRRQQFLIRSLGGFGDCAHQNRRQQIKAMSGTSMEADAENILRAITPSLDPKR 60

Query: 61  YKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPI 120
           YKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHP+
Sbjct: 61  YKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPV 120

Query: 121 LEESYSVRDEEKKLIAERVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHARQTN 180
           LEESYSVRDEEKK  AERVLAEVDKWLERFDCLVIGPGLGRDPFLL+CVSEIIKHARQTN
Sbjct: 121 LEESYSVRDEEKKFTAERVLAEVDKWLERFDCLVIGPGLGRDPFLLECVSEIIKHARQTN 180

Query: 181 ITMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKKVLLVEVDEQDAPNQLLSLA 240
           I M+IDGDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVK VLL EVDEQDAPNQLLSLA
Sbjct: 181 IPMIIDGDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKNVLLAEVDEQDAPNQLLSLA 240

Query: 241 KRIGGLTILRKGRADLISDGETVKSVSIYGSPRRCGGQGDILSGRQVLPCVFIILGEYVF 300
           KRIGGLTILRKGRADLI DGETVKSVSIYGSPRRCGGQGDILSG              VF
Sbjct: 241 KRIGGLTILRKGRADLICDGETVKSVSIYGSPRRCGGQGDILSGSVA-----------VF 300

Query: 301 VSWARRQGLVTDGNLAT 318
           VSWAR+QGLVTDGNLAT
Sbjct: 301 VSWARQQGLVTDGNLAT 306

BLAST of Lag0008665 vs. ExPASy TrEMBL
Match: A0A6J1ITH2 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Cucurbita maxima OX=3661 GN=LOC111479195 PE=3 SV=1)

HSP 1 Score: 564.7 bits (1454), Expect = 2.7e-157
Identity = 287/317 (90.54%), Postives = 294/317 (92.74%), Query Frame = 0

Query: 1   MLASSAVFRRQQFLIRSLGGFGDCTHQNRQQQVKAMSGASIEADAENILRAITPSLDPNR 60
           MLASSAVFRRQQFLIRSLGGFGDCTHQNR+QQ+KAMSG S+EADAENILRAITPSLDP R
Sbjct: 1   MLASSAVFRRQQFLIRSLGGFGDCTHQNRRQQIKAMSGTSMEADAENILRAITPSLDPKR 60

Query: 61  YKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPI 120
           +KGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHP+
Sbjct: 61  HKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPV 120

Query: 121 LEESYSVRDEEKKLIAERVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHARQTN 180
           LEESYSVRDEEKK  AERVLAEVDKWLERFDCLVIGPGLGRDPFLL+CVSEIIKHARQTN
Sbjct: 121 LEESYSVRDEEKKFTAERVLAEVDKWLERFDCLVIGPGLGRDPFLLECVSEIIKHARQTN 180

Query: 181 ITMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKKVLLVEVDEQDAPNQLLSLA 240
           I MVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVK VLL EVDEQDAPNQLLSLA
Sbjct: 181 IPMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKNVLLAEVDEQDAPNQLLSLA 240

Query: 241 KRIGGLTILRKGRADLISDGETVKSVSIYGSPRRCGGQGDILSGRQVLPCVFIILGEYVF 300
           KRIGGLTILRKGRADLI DGETVKSVSIYGSPRRCGGQGDILSG              VF
Sbjct: 241 KRIGGLTILRKGRADLICDGETVKSVSIYGSPRRCGGQGDILSGSVA-----------VF 300

Query: 301 VSWARRQGLVTDGNLAT 318
           VSWAR+QGLV DGNLAT
Sbjct: 301 VSWARQQGLVIDGNLAT 306

BLAST of Lag0008665 vs. ExPASy TrEMBL
Match: A0A5A7U2C1 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold171G006330 PE=3 SV=1)

HSP 1 Score: 563.1 bits (1450), Expect = 7.9e-157
Identity = 283/317 (89.27%), Postives = 297/317 (93.69%), Query Frame = 0

Query: 1   MLASSAVFRRQQFLIRSLGGFGDCTHQNRQQQVKAMSGASIEADAENILRAITPSLDPNR 60
           MLAS AVFRRQQFL+R LGGFGDCT+QNR+QQ+KAMSG SIEADAE+ILRAITP LDPNR
Sbjct: 1   MLASPAVFRRQQFLLRCLGGFGDCTYQNRRQQIKAMSGTSIEADAEHILRAITPCLDPNR 60

Query: 61  YKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPI 120
           YKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHP+
Sbjct: 61  YKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPV 120

Query: 121 LEESYSVRDEEKKLIAERVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHARQTN 180
           LEESYSVRDEEKK I+ERVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHARQTN
Sbjct: 121 LEESYSVRDEEKKFISERVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHARQTN 180

Query: 181 ITMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKKVLLVEVDEQDAPNQLLSLA 240
           I MVIDGDGLFLIT NLHLVSNYPLAVLTPNVNEYKRLV+ VLL EVDEQDAPNQLLSLA
Sbjct: 181 IPMVIDGDGLFLITGNLHLVSNYPLAVLTPNVNEYKRLVENVLLAEVDEQDAPNQLLSLA 240

Query: 241 KRIGGLTILRKGRADLISDGETVKSVSIYGSPRRCGGQGDILSGRQVLPCVFIILGEYVF 300
           KRIGG+TILRKGRADLISDGETVKSVSIYGSPRRCGGQGDILSGRQV+  V       VF
Sbjct: 241 KRIGGITILRKGRADLISDGETVKSVSIYGSPRRCGGQGDILSGRQVIGIV------AVF 300

Query: 301 VSWARRQGLVTDGNLAT 318
           +SWA+RQGLVTD N+ +
Sbjct: 301 ISWAQRQGLVTDDNMTS 311

BLAST of Lag0008665 vs. ExPASy TrEMBL
Match: A0A5D3CYW0 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold434G00150 PE=3 SV=1)

HSP 1 Score: 553.9 bits (1426), Expect = 4.8e-154
Identity = 279/317 (88.01%), Postives = 292/317 (92.11%), Query Frame = 0

Query: 1   MLASSAVFRRQQFLIRSLGGFGDCTHQNRQQQVKAMSGASIEADAENILRAITPSLDPNR 60
           MLAS AVFRRQQFL+R LGGFGDCT+QNR+QQ+KAMSG SIEADAE+ILRAITP LDPNR
Sbjct: 1   MLASPAVFRRQQFLLRCLGGFGDCTYQNRRQQIKAMSGTSIEADAEHILRAITPCLDPNR 60

Query: 61  YKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPI 120
           YKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHP+
Sbjct: 61  YKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPV 120

Query: 121 LEESYSVRDEEKKLIAERVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHARQTN 180
           LEESYSVRDEEKK I+ERVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHARQTN
Sbjct: 121 LEESYSVRDEEKKFISERVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHARQTN 180

Query: 181 ITMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKKVLLVEVDEQDAPNQLLSLA 240
           I MVIDGDGLFLIT NLHLVSNYPLAVLTPNVNEYKRLV+ VLL EVDEQDAPNQLLSLA
Sbjct: 181 IPMVIDGDGLFLITGNLHLVSNYPLAVLTPNVNEYKRLVENVLLAEVDEQDAPNQLLSLA 240

Query: 241 KRIGGLTILRKGRADLISDGETVKSVSIYGSPRRCGGQGDILSGRQVLPCVFIILGEYVF 300
           KRIGG+TILRKGRADLISDGETVKSVSIYGSPRRCGGQGDILSG              VF
Sbjct: 241 KRIGGITILRKGRADLISDGETVKSVSIYGSPRRCGGQGDILSGSVA-----------VF 300

Query: 301 VSWARRQGLVTDGNLAT 318
           +SWA+RQGLVTD N+ +
Sbjct: 301 ISWAQRQGLVTDDNMTS 306

BLAST of Lag0008665 vs. ExPASy TrEMBL
Match: A0A1S3AX65 (ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Cucumis melo OX=3656 GN=LOC103483736 PE=3 SV=1)

HSP 1 Score: 553.5 bits (1425), Expect = 6.3e-154
Identity = 279/317 (88.01%), Postives = 292/317 (92.11%), Query Frame = 0

Query: 1   MLASSAVFRRQQFLIRSLGGFGDCTHQNRQQQVKAMSGASIEADAENILRAITPSLDPNR 60
           MLAS AVFRRQQFL+R LGGFGDCT+QNR+QQ+KAMSG SIEADAE+ILRAITP LDPNR
Sbjct: 1   MLASPAVFRRQQFLLRCLGGFGDCTYQNRRQQIKAMSGTSIEADAEHILRAITPCLDPNR 60

Query: 61  YKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPI 120
           YKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHP+
Sbjct: 61  YKGQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPV 120

Query: 121 LEESYSVRDEEKKLIAERVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHARQTN 180
           LEESYSVRDEE+K I+ERVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHARQTN
Sbjct: 121 LEESYSVRDEERKFISERVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHARQTN 180

Query: 181 ITMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKKVLLVEVDEQDAPNQLLSLA 240
           I MVIDGDGLFLIT NLHLVSNYPLAVLTPNVNEYKRLV+ VLL EVDEQDAPNQLLSLA
Sbjct: 181 IPMVIDGDGLFLITGNLHLVSNYPLAVLTPNVNEYKRLVENVLLAEVDEQDAPNQLLSLA 240

Query: 241 KRIGGLTILRKGRADLISDGETVKSVSIYGSPRRCGGQGDILSGRQVLPCVFIILGEYVF 300
           KRIGGLTILRKGRADLISDGETVKSVSIYGSPRRCGGQGDILSG              VF
Sbjct: 241 KRIGGLTILRKGRADLISDGETVKSVSIYGSPRRCGGQGDILSGSVA-----------VF 300

Query: 301 VSWARRQGLVTDGNLAT 318
           +SWA+RQGLVTD N+ +
Sbjct: 301 ISWAQRQGLVTDDNMTS 306

BLAST of Lag0008665 vs. TAIR 10
Match: AT5G19150.1 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 410.2 bits (1053), Expect = 1.6e-114
Identity = 213/307 (69.38%), Postives = 255/307 (83.06%), Query Frame = 0

Query: 3   ASSAVFRRQQFLIRSLGGFGDCTHQNRQQQVKAMSGASIEADAENILRAITPSLDPNRYK 62
           +SS+V RRQ+FL+R+L G            ++AMS  S EADAE++LR +TPSLD  R+K
Sbjct: 21  SSSSVLRRQEFLVRTLCG---------SPIIRAMSSTS-EADAESVLRTVTPSLDLKRHK 80

Query: 63  GQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPILE 122
           GQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHP+LE
Sbjct: 81  GQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPVLE 140

Query: 123 ESYSV---RDEEKKLIAERVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHARQT 182
           ESYS+    +E+K+ + ++VL EV KW+ERFDCLVIGPGLGRDPFLL+CVS I+  A+++
Sbjct: 141 ESYSISQLSEEDKREVQDKVLGEVGKWMERFDCLVIGPGLGRDPFLLECVSIIMLLAKKS 200

Query: 183 NITMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKKVLLVEVDEQDAPNQLLSL 242
           N+  VIDGDGLFL+T+++ LV +YPLAVLTPNVNEYKRLV+KVL  EVDEQ+A +QL SL
Sbjct: 201 NVPFVIDGDGLFLVTNSIDLVHSYPLAVLTPNVNEYKRLVQKVLNCEVDEQNAEDQLRSL 260

Query: 243 AKRIGGLTILRKGRADLISDGETVKSVSIYGSPRRCGGQGDILSGRQVLPCVFIILGEYV 302
           AK+IGG+TILRKG++DLIS+GETVKSVSIYGSPRRCGGQGDILSG           G  V
Sbjct: 261 AKQIGGVTILRKGKSDLISNGETVKSVSIYGSPRRCGGQGDILSG-----------GVAV 306

Query: 303 FVSWARR 307
           F+SWA++
Sbjct: 321 FLSWAQQ 306

BLAST of Lag0008665 vs. TAIR 10
Match: AT5G19150.2 (pfkB-like carbohydrate kinase family protein )

HSP 1 Score: 410.2 bits (1053), Expect = 1.6e-114
Identity = 213/307 (69.38%), Postives = 255/307 (83.06%), Query Frame = 0

Query: 3   ASSAVFRRQQFLIRSLGGFGDCTHQNRQQQVKAMSGASIEADAENILRAITPSLDPNRYK 62
           +SS+V RRQ+FL+R+L G            ++AMS  S EADAE++LR +TPSLD  R+K
Sbjct: 21  SSSSVLRRQEFLVRTLCG---------SPIIRAMSSTS-EADAESVLRTVTPSLDLKRHK 80

Query: 63  GQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPILE 122
           GQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHP+LE
Sbjct: 81  GQAGKIAVIGGCREYTGAPYFAAISALKIGADLSHVFCTKDAAPVIKSYSPELIVHPVLE 140

Query: 123 ESYSV---RDEEKKLIAERVLAEVDKWLERFDCLVIGPGLGRDPFLLDCVSEIIKHARQT 182
           ESYS+    +E+K+ + ++VL EV KW+ERFDCLVIGPGLGRDPFLL+CVS I+  A+++
Sbjct: 141 ESYSISQLSEEDKREVQDKVLGEVGKWMERFDCLVIGPGLGRDPFLLECVSIIMLLAKKS 200

Query: 183 NITMVIDGDGLFLITDNLHLVSNYPLAVLTPNVNEYKRLVKKVLLVEVDEQDAPNQLLSL 242
           N+  VIDGDGLFL+T+++ LV +YPLAVLTPNVNEYKRLV+KVL  EVDEQ+A +QL SL
Sbjct: 201 NVPFVIDGDGLFLVTNSIDLVHSYPLAVLTPNVNEYKRLVQKVLNCEVDEQNAEDQLRSL 260

Query: 243 AKRIGGLTILRKGRADLISDGETVKSVSIYGSPRRCGGQGDILSGRQVLPCVFIILGEYV 302
           AK+IGG+TILRKG++DLIS+GETVKSVSIYGSPRRCGGQGDILSG           G  V
Sbjct: 261 AKQIGGVTILRKGKSDLISNGETVKSVSIYGSPRRCGGQGDILSG-----------GVAV 306

Query: 303 FVSWARR 307
           F+SWA++
Sbjct: 321 FLSWAQQ 306

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023528365.11.1e-15790.54ATP-dependent (S)-NAD(P)H-hydrate dehydratase [Cucurbita pepo subsp. pepo][more]
XP_022955699.11.5e-15790.54ATP-dependent (S)-NAD(P)H-hydrate dehydratase [Cucurbita moschata][more]
XP_038878236.14.3e-15790.54ATP-dependent (S)-NAD(P)H-hydrate dehydratase isoform X1 [Benincasa hispida][more]
XP_022979515.15.6e-15790.54ATP-dependent (S)-NAD(P)H-hydrate dehydratase [Cucurbita maxima][more]
KAG7018682.17.3e-15784.39ATP-dependent (S)-NAD(P)H-hydrate dehydratase [Cucurbita argyrosperma subsp. arg... [more]
Match NameE-valueIdentityDescription
F6HDM21.0e-12473.97ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Vitis vinifera OX=29760 GN=VIT_... [more]
Q94AF22.3e-11369.38ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Arabidopsis thaliana OX=3702 GN... [more]
C5Y2108.0e-10663.04ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Sorghum bicolor OX=4558 GN=Sb05... [more]
A9RY032.9e-8762.08ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Physcomitrium patens OX=3218 GN... [more]
E1BNQ46.8e-6551.12ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Bos taurus OX=9913 GN=NAXD PE=3... [more]
Match NameE-valueIdentityDescription
A0A6J1GX137.1e-15890.54ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Cucurbita moschata OX=3662 GN=L... [more]
A0A6J1ITH22.7e-15790.54ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Cucurbita maxima OX=3661 GN=LOC... [more]
A0A5A7U2C17.9e-15789.27ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Cucumis melo var. makuwa OX=119... [more]
A0A5D3CYW04.8e-15488.01ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Cucumis melo var. makuwa OX=119... [more]
A0A1S3AX656.3e-15488.01ATP-dependent (S)-NAD(P)H-hydrate dehydratase OS=Cucumis melo OX=3656 GN=LOC1034... [more]
Match NameE-valueIdentityDescription
AT5G19150.11.6e-11469.38pfkB-like carbohydrate kinase family protein [more]
AT5G19150.21.6e-11469.38pfkB-like carbohydrate kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000631ATP-dependent (S)-NAD(P)H-hydrate dehydrataseTIGRFAMTIGR00196TIGR00196coord: 51..284
e-value: 2.8E-46
score: 156.0
IPR000631ATP-dependent (S)-NAD(P)H-hydrate dehydratasePFAMPF01256Carb_kinasecoord: 69..284
e-value: 1.6E-43
score: 149.0
IPR000631ATP-dependent (S)-NAD(P)H-hydrate dehydrataseHAMAPMF_01965NADHX_dehydratasecoord: 45..349
score: 24.683163
IPR000631ATP-dependent (S)-NAD(P)H-hydrate dehydratasePROSITEPS51383YJEF_C_3coord: 45..351
score: 64.586182
IPR000631ATP-dependent (S)-NAD(P)H-hydrate dehydrataseCDDcd01171YXKO-relatedcoord: 58..284
e-value: 3.22704E-74
score: 228.266
IPR029056Ribokinase-likeGENE3D3.40.1190.20coord: 44..286
e-value: 5.4E-69
score: 234.6
IPR029056Ribokinase-likeSUPERFAMILY53613Ribokinase-likecoord: 47..284
NoneNo IPR availablePANTHERPTHR12592:SF1ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASEcoord: 3..286
NoneNo IPR availablePANTHERPTHR12592ATP-DEPENDENT (S)-NAD(P)H-HYDRATE DEHYDRATASE FAMILY MEMBERcoord: 3..286

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0008665.1Lag0008665.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0110051 metabolite repair
biological_process GO:0046496 nicotinamide nucleotide metabolic process
molecular_function GO:0052855 ADP-dependent NAD(P)H-hydrate dehydratase activity
molecular_function GO:0005524 ATP binding
molecular_function GO:0047453 ATP-dependent NAD(P)H-hydrate dehydratase activity