Lag0008623 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0008623
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionWRKY protein
Locationchr9: 26643106 .. 26644213 (+)
RNA-Seq ExpressionLag0008623
SyntenyLag0008623
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGATCATCATCGTCAGAGATAACAGTGAACCAGTTGGTGTTGCCGAGTCCATTGCAACCCCAACCTGCCGCCGACGCCTCATCCCCCTCTCCGATAATTCAACAATCTCTTCCTAGGTTCCTCTTTATATTTATTTCGTTTTTTTTGTTTTATACTTATACTCTCCGTTCAACTGGTTAAGATATTATACATCTAATTTCTATAGGGATAATGTTGGGGAGAGCGGCGGCAACGATAACGGTGACCGTGACGTGTGTAAGCAGCCGTTGAGGGTGTCGACGAGGGAGATGAAGGTGAGGAAGAGAGAAGGGAGGAAGAAAGTGGCTGAGCCAAAAATCTACGTCATAATCAAGAGCGATGTGGATGTTCTTGAAGATGGTTTCAAATGGAGAAAGTATGGCCAAAAAGTTGCTAAGAACTCCCTCCATCCCAGGTTTGTTTCACATTTCACCTTTTTCGAAAAATTTCTACTACTAGGGTAGGAAAATTTAAGATCCGTCACAATGCTCTAGGACAGATAAATTTGAAGAGAGGAAAATTTAAGATCCGACACAATGCTCTAGGACAGAAAATTAACTGAAGCGTTAAAAAATTTCACACATTTTTCTACCTTTCTCTTTGTTCATTCTGATGATAATTTTGGAGTTTCTAATTATTTTTTTCATTATTGACTACTTTTTGAGAATTATTCGGAAATAATTGAAAAAAAAAAAATTCAGATTTGTACAAGTGTGATTCAATGGGGTTTTAATCTGGACTTTGGACTTTGACAAACCGATATATGATATCTGAGTTTCTCTATATCAAAATAATGCTATATATTAGTGAAGAATAAGCTTCTTTTATTCTATTAGAGTAGGAAAATACTACATACATTGGTATTCGCTATGGTATTTTAATTTAGACTTTAACCAATACAAACATGTGCAGGAATTACTATCGTTGTACACAAGAGAATTGTAAGGTAAAAAAGCGAGTAACAAGATTGGATAAAGATCCAAGAATGGTATCGACACTCTATCGAGGAAGACATATTCATTCAATTCCATCTCAAGAGACAATGGATCAGCCACGGTCGGGCCATCCTAACAATTTCTTCTGCTAA

mRNA sequence

ATGGGATCATCATCGTCAGAGATAACAGTGAACCAGTTGGTGTTGCCGAGTCCATTGCAACCCCAACCTGCCGCCGACGCCTCATCCCCCTCTCCGATAATTCAACAATCTCTTCCTAGGGATAATGTTGGGGAGAGCGGCGGCAACGATAACGGTGACCGTGACGTGTGTAAGCAGCCGTTGAGGGTGTCGACGAGGGAGATGAAGGTGAGGAAGAGAGAAGGGAGGAAGAAAGTGGCTGAGCCAAAAATCTACGTCATAATCAAGAGCGATGTGGATGTTCTTGAAGATGGTTTCAAATGGAGAAAGTATGGCCAAAAAGTTGCTAAGAACTCCCTCCATCCCAGGAATTACTATCGTTGTACACAAGAGAATTGTAAGGTAAAAAAGCGAGTAACAAGATTGGATAAAGATCCAAGAATGGTATCGACACTCTATCGAGGAAGACATATTCATTCAATTCCATCTCAAGAGACAATGGATCAGCCACGGTCGGGCCATCCTAACAATTTCTTCTGCTAA

Coding sequence (CDS)

ATGGGATCATCATCGTCAGAGATAACAGTGAACCAGTTGGTGTTGCCGAGTCCATTGCAACCCCAACCTGCCGCCGACGCCTCATCCCCCTCTCCGATAATTCAACAATCTCTTCCTAGGGATAATGTTGGGGAGAGCGGCGGCAACGATAACGGTGACCGTGACGTGTGTAAGCAGCCGTTGAGGGTGTCGACGAGGGAGATGAAGGTGAGGAAGAGAGAAGGGAGGAAGAAAGTGGCTGAGCCAAAAATCTACGTCATAATCAAGAGCGATGTGGATGTTCTTGAAGATGGTTTCAAATGGAGAAAGTATGGCCAAAAAGTTGCTAAGAACTCCCTCCATCCCAGGAATTACTATCGTTGTACACAAGAGAATTGTAAGGTAAAAAAGCGAGTAACAAGATTGGATAAAGATCCAAGAATGGTATCGACACTCTATCGAGGAAGACATATTCATTCAATTCCATCTCAAGAGACAATGGATCAGCCACGGTCGGGCCATCCTAACAATTTCTTCTGCTAA

Protein sequence

MGSSSSEITVNQLVLPSPLQPQPAADASSPSPIIQQSLPRDNVGESGGNDNGDRDVCKQPLRVSTREMKVRKREGRKKVAEPKIYVIIKSDVDVLEDGFKWRKYGQKVAKNSLHPRNYYRCTQENCKVKKRVTRLDKDPRMVSTLYRGRHIHSIPSQETMDQPRSGHPNNFFC
Homology
[Close]
BLAST of Lag0008623 vs. NCBI nr
Match: XP_023544832.1 (probable WRKY transcription factor 12 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 150.2 bits (378), Expect = 1.6e-32
Identity = 86/162 (53.09%), Postives = 109/162 (67.28%), Query Frame = 0 

Query: 12  QLVLPSPLQPQPAADASSPSPIIQQSLPRDNVGESGGNDNGDRDVCKQPLRVSTREMKVR 71
           Q V P P  P P+  A++ S ++  S  RD  GESG + + D D  K  LRV  R +K++
Sbjct: 65  QSVFPFPF-PFPSTTATATSHLL--SSLRD-AGESGNHGDCDGDNRKGGLRV--RTVKMK 124

Query: 72  KREGRKKVAEPKIYVIIKSDVDVLEDGFKWRKYGQKVAKNSLHPRNYYRCTQENCKVKKR 131
           + +GR+KV EP+      +DVDVL+DG+KWRKYGQKV KN+LHPR+YYRCT+ENCKVKKR
Sbjct: 125 RMKGRRKVREPRFCFKTMTDVDVLDDGYKWRKYGQKVVKNTLHPRSYYRCTEENCKVKKR 184

Query: 132 VTRLDKDPRMVSTLYRGRHIHSIPSQETMDQPRSGHPNNFFC 174
           V RL +DPRMV T Y GRH HS PS   ++    G  N FFC
Sbjct: 185 VERLAEDPRMVITTYEGRHAHS-PSDRNLEDTNMGCLNTFFC 219

[Close]
BLAST of Lag0008623 vs. NCBI nr
Match: XP_022977891.1 (probable WRKY transcription factor 12 [Cucurbita maxima])

HSP 1 Score: 150.2 bits (378), Expect = 1.6e-32
Identity = 81/167 (48.50%), Postives = 107/167 (64.07%), Query Frame = 0 

Query: 12  QLVLPSPLQPQPAADASSPSPIIQQSLPRD-----NVGESGGNDNGDRDVCKQPLRVSTR 71
           Q V P P  P P+  A++ S ++   L        + GESG + +GD D   +   +  R
Sbjct: 64  QSVFPFPF-PFPSTTATATSHLLSSLLRSSPSSLRDAGESGNHGDGDGDGDNRKGGLRVR 123

Query: 72  EMKVRKREGRKKVAEPKIYVIIKSDVDVLEDGFKWRKYGQKVAKNSLHPRNYYRCTQENC 131
            +K+++ +GR+KV EP+      +DVDVL+DG+KWRKYGQKV KN+LHPR+YYRCT+ENC
Sbjct: 124 TVKMKRMKGRRKVREPRFCFKTMTDVDVLDDGYKWRKYGQKVVKNTLHPRSYYRCTEENC 183

Query: 132 KVKKRVTRLDKDPRMVSTLYRGRHIHSIPSQETMDQPRSGHPNNFFC 174
           KVKKRV RL +DPRMV T Y GRH HS PS   ++    G  N FFC
Sbjct: 184 KVKKRVERLAEDPRMVITTYEGRHAHS-PSDRNLEDTNMGCLNTFFC 228

[Close]
BLAST of Lag0008623 vs. NCBI nr
Match: XP_016899208.1 (PREDICTED: probable WRKY transcription factor 12 isoform X1 [Cucumis melo])

HSP 1 Score: 148.7 bits (374), Expect = 4.7e-32
Identity = 79/139 (56.83%), Postives = 98/139 (70.50%), Query Frame = 0 

Query: 40  RDNVGESGGNDNGDRDVCKQPLRVSTREMKVRKREGRKKVAEPKIYVIIKSDVDVLEDGF 99
           R+ +G  GG+D+ + D  K  LRVST  MK+++ +GRKKV EP+      +DVDVL+DG+
Sbjct: 113 RNAIGNYGGDDDKEND-GKPRLRVST--MKMKRIKGRKKVREPRFSFKTMTDVDVLDDGY 172

Query: 100 KWRKYGQKVAKNSLHPRNYYRCTQENCKVKKRVTRLDKDPRMVSTLYRGRHIHSIPSQET 159
           KWRKYGQKV KN+LHPR+YYRCT+ENCKVKKRV RL  DPRMV T Y GRH HS PS   
Sbjct: 173 KWRKYGQKVVKNTLHPRSYYRCTEENCKVKKRVERLADDPRMVITTYEGRHAHS-PSDHN 232

Query: 160 MDQP-----RSGHPNNFFC 174
           ++ P      S H  +FFC
Sbjct: 233 LEDPIMGHLPSSHLTSFFC 247

[Close]
BLAST of Lag0008623 vs. NCBI nr
Match: XP_008440122.1 (PREDICTED: probable WRKY transcription factor 12 isoform X2 [Cucumis melo])

HSP 1 Score: 148.7 bits (374), Expect = 4.7e-32
Identity = 79/139 (56.83%), Postives = 98/139 (70.50%), Query Frame = 0 

Query: 40  RDNVGESGGNDNGDRDVCKQPLRVSTREMKVRKREGRKKVAEPKIYVIIKSDVDVLEDGF 99
           R+ +G  GG+D+ + D  K  LRVST  MK+++ +GRKKV EP+      +DVDVL+DG+
Sbjct: 103 RNAIGNYGGDDDKEND-GKPRLRVST--MKMKRIKGRKKVREPRFSFKTMTDVDVLDDGY 162

Query: 100 KWRKYGQKVAKNSLHPRNYYRCTQENCKVKKRVTRLDKDPRMVSTLYRGRHIHSIPSQET 159
           KWRKYGQKV KN+LHPR+YYRCT+ENCKVKKRV RL  DPRMV T Y GRH HS PS   
Sbjct: 163 KWRKYGQKVVKNTLHPRSYYRCTEENCKVKKRVERLADDPRMVITTYEGRHAHS-PSDHN 222

Query: 160 MDQP-----RSGHPNNFFC 174
           ++ P      S H  +FFC
Sbjct: 223 LEDPIMGHLPSSHLTSFFC 237

[Close]
BLAST of Lag0008623 vs. NCBI nr
Match: XP_038883389.1 (probable WRKY transcription factor 13 [Benincasa hispida])

HSP 1 Score: 146.7 bits (369), Expect = 1.8e-31
Identity = 77/139 (55.40%), Postives = 95/139 (68.35%), Query Frame = 0 

Query: 40  RDNVGESGGNDNGDRDVCKQPLRVSTREMKVRKREGRKKVAEPKIYVIIKSDVDVLEDGF 99
           R+ +G  GG D  D D  K  LR+ST  +K+++ +GRKKV EP+      +DVDVL+DG+
Sbjct: 109 RNGIGNYGG-DGDDNDDRKPQLRIST--LKMKRIKGRKKVREPRFSFKTMTDVDVLDDGY 168

Query: 100 KWRKYGQKVAKNSLHPRNYYRCTQENCKVKKRVTRLDKDPRMVSTLYRGRHIHSIPSQET 159
           KWRKYGQK+ KN+LHPR+YYRCT+ENCKVKKRV RL  DPRMV T Y GRH HS PS   
Sbjct: 169 KWRKYGQKIVKNTLHPRSYYRCTEENCKVKKRVERLADDPRMVITTYEGRHAHS-PSDHN 228

Query: 160 MDQP-----RSGHPNNFFC 174
           ++        S H  NFFC
Sbjct: 229 VEDSIMCHLPSSHLTNFFC 243

[Close]
BLAST of Lag0008623 vs. ExPASy Swiss-Prot
Match: Q9SVB7 (Probable WRKY transcription factor 13 OS=Arabidopsis thaliana OX=3702 GN=WRKY13 PE=1 SV=1)

HSP 1 Score: 122.9 bits (307), Expect = 3.6e-27
Identity = 72/149 (48.32%), Postives = 90/149 (60.40%), Query Frame = 0 

Query: 41  DNVGESGG---NDNGDRDVCKQPLRVS---------TREMKVRKREGRKKVAEPKIYVII 100
           D VG+ GG   +DNG       P R              +K++K + R+KV EP+     
Sbjct: 156 DEVGDGGGINDDDNGRHHHHDTPSRHDKHNTASLGVVSSLKMKKLKTRRKVREPRFCFKT 215

Query: 101 KSDVDVLEDGFKWRKYGQKVAKNSLHPRNYYRCTQENCKVKKRVTRLDKDPRMVSTLYRG 160
            S+VDVL+DG++WRKYGQKV KN+ HPR+YYRCTQ+ C+VKKRV RL  DPRMV T Y G
Sbjct: 216 LSEVDVLDDGYRWRKYGQKVVKNTQHPRSYYRCTQDKCRVKKRVERLADDPRMVITTYEG 275

Query: 161 RHIHSIPSQETMDQPRSG---HP--NNFF 173
           RH+HS PS    D   S    HP  +NFF
Sbjct: 276 RHLHS-PSNHLDDDSLSTSHLHPPLSNFF 303

[Close]
BLAST of Lag0008623 vs. ExPASy Swiss-Prot
Match: Q93WY4 (Probable WRKY transcription factor 12 OS=Arabidopsis thaliana OX=3702 GN=WRKY12 PE=1 SV=1)

HSP 1 Score: 116.3 bits (290), Expect = 3.4e-25
Identity = 64/128 (50.00%), Postives = 81/128 (63.28%), Query Frame = 0 

Query: 32  PIIQQSLPRDNVGESGGNDNGDRDVCKQPLRVSTREMKVRKREGRKKVAEPKIYVIIKSD 91
           P+  + +  D     GG +N             + +MK  K + R+K+ EP+     KSD
Sbjct: 82  PLGSKVVNDDQENFGGGTNNDAHSNSWWRSNSGSGDMK-NKVKIRRKLREPRFCFQTKSD 141

Query: 92  VDVLEDGFKWRKYGQKVAKNSLHPRNYYRCTQENCKVKKRVTRLDKDPRMVSTLYRGRHI 151
           VDVL+DG+KWRKYGQKV KNSLHPR+YYRCT  NC+VKKRV RL +D RMV T Y GRH 
Sbjct: 142 VDVLDDGYKWRKYGQKVVKNSLHPRSYYRCTHNNCRVKKRVERLSEDCRMVITTYEGRHN 201

Query: 152 HSIPSQET 160
           H IPS ++
Sbjct: 202 H-IPSDDS 207

[Close]
BLAST of Lag0008623 vs. ExPASy Swiss-Prot
Match: Q8VWJ2 (WRKY transcription factor 28 OS=Arabidopsis thaliana OX=3702 GN=WRKY28 PE=2 SV=1)

HSP 1 Score: 112.1 bits (279), Expect = 6.4e-24
Identity = 58/126 (46.03%), Postives = 79/126 (62.70%), Query Frame = 0 

Query: 31  SPIIQQSLPRDNVGESGGNDNGDRDVCKQPLRVSTREMKVRKREGRKKVAEPKIYVIIKS 90
           SP    S   D+ GE  G     R++  +  ++S +  K +K E  KK  EP++  + KS
Sbjct: 108 SPSNSSSSEADHPGEDSGKSRRKRELVGEEDQISKKVGKTKKTE-VKKQREPRVSFMTKS 167

Query: 91  DVDVLEDGFKWRKYGQKVAKNSLHPRNYYRCTQENCKVKKRVTRLDKDPRMVSTLYRGRH 150
           +VD LEDG++WRKYGQK  KNS +PR+YYRCT + C VKKRV R  +DP +V T Y G+H
Sbjct: 168 EVDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTTQKCNVKKRVERSFQDPTVVITTYEGQH 227

Query: 151 IHSIPS 157
            H IP+
Sbjct: 228 NHPIPT 232

[Close]
BLAST of Lag0008623 vs. ExPASy Swiss-Prot
Match: Q93WV4 (WRKY transcription factor 71 OS=Arabidopsis thaliana OX=3702 GN=WRKY71 PE=2 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 1.2e-22
Identity = 62/147 (42.18%), Postives = 87/147 (59.18%), Query Frame = 0 

Query: 17  SPLQPQPAADASSPSPIIQQSLPRDNVGESGGNDNGDRDVCK------QPLRVSTREM-K 76
           SP    P  + +S SP++  S    N GE   N N   D  +        +  S++++ K
Sbjct: 53  SPSVNNPYLNLTSNSPVVSSS---SNEGEPKENTNDKSDQMEDNEGDLHGVGESSKQLTK 112

Query: 77  VRKREGRKKVAEPKIYVIIKSDVDVLEDGFKWRKYGQKVAKNSLHPRNYYRCTQENCKVK 136
             K++G KK  E ++  + KS++D LEDG++WRKYGQK  KNS +PR+YYRCT + C VK
Sbjct: 113 QGKKKGEKKEREVRVAFMTKSEIDHLEDGYRWRKYGQKAVKNSPYPRSYYRCTTQKCNVK 172

Query: 137 KRVTRLDKDPRMVSTLYRGRHIHSIPS 157
           KRV R  +DP +V T Y G+H H IPS
Sbjct: 173 KRVERSFQDPSIVITTYEGKHNHPIPS 196

[Close]
BLAST of Lag0008623 vs. ExPASy Swiss-Prot
Match: O22900 (WRKY transcription factor 23 OS=Arabidopsis thaliana OX=3702 GN=WRKY23 PE=2 SV=1)

HSP 1 Score: 104.4 bits (259), Expect = 1.3e-21
Identity = 57/154 (37.01%), Postives = 87/154 (56.49%), Query Frame = 0 

Query: 3   SSSSEITVNQLVLPSPLQPQPAADASSPSPIIQQSLPR-DNVGESGGNDNGDRDVCKQPL 62
           S    +  + L++  P  P  ++ +S+ S  + +  P+ ++  E GG D  ++   K+ L
Sbjct: 88  SKVESLCSDHLLINPPATPNSSSISSASSEALNEEKPKTEDNEEEGGEDQQEKSHTKKQL 147

Query: 63  RVSTREMKVRKREGRKKVAEPKIYVIIKSDVDVLEDGFKWRKYGQKVAKNSLHPRNYYRC 122
           +         K+  +K+  E ++  + KS+VD LEDG++WRKYGQK  KNS  PR+YYRC
Sbjct: 148 KA--------KKNNQKRQREARVAFMTKSEVDHLEDGYRWRKYGQKAVKNSPFPRSYYRC 207

Query: 123 TQENCKVKKRVTRLDKDPRMVSTLYRGRHIHSIP 156
           T  +C VKKRV R  +DP  V T Y G+H H  P
Sbjct: 208 TTASCNVKKRVERSFRDPSTVVTTYEGQHTHISP 233

The following BLAST results are available for this feature:
BLAST of Lag0008623 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_023544832.11.6e-3253.09probable WRKY transcription factor 12 [Cucurbita pepo subsp. pepo][more]
XP_022977891.11.6e-3248.50probable WRKY transcription factor 12 [Cucurbita maxima][more]
XP_016899208.14.7e-3256.83PREDICTED: probable WRKY transcription factor 12 isoform X1 [Cucumis melo][more]
XP_008440122.14.7e-3256.83PREDICTED: probable WRKY transcription factor 12 isoform X2 [Cucumis melo][more]
XP_038883389.11.8e-3155.40probable WRKY transcription factor 13 [Benincasa hispida][more]
BLAST of Lag0008623 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q9SVB73.6e-2748.32Probable WRKY transcription factor 13 OS=Arabidopsis thaliana OX=3702 GN=WRKY13 ... [more]
Q93WY43.4e-2550.00Probable WRKY transcription factor 12 OS=Arabidopsis thaliana OX=3702 GN=WRKY12 ... [more]
Q8VWJ26.4e-2446.03WRKY transcription factor 28 OS=Arabidopsis thaliana OX=3702 GN=WRKY28 PE=2 SV=1[more]
Q93WV41.2e-2242.18WRKY transcription factor 71 OS=Arabidopsis thaliana OX=3702 GN=WRKY71 PE=2 SV=1[more]
O229001.3e-2137.01WRKY transcription factor 23 OS=Arabidopsis thaliana OX=3702 GN=WRKY23 PE=2 SV=1[more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003657WRKY domainSMARTSM00774WRKY_clscoord: 95..154
e-value: 4.0E-32
score: 122.7
IPR003657WRKY domainPFAMPF03106WRKYcoord: 96..152
e-value: 6.9E-23
score: 80.7
IPR003657WRKY domainPROSITEPS50811WRKYcoord: 90..155
score: 26.876688
IPR036576WRKY domain superfamilyGENE3D2.20.25.80WRKY domaincoord: 80..156
e-value: 5.3E-29
score: 101.8
IPR036576WRKY domain superfamilySUPERFAMILY118290WRKY DNA-binding domaincoord: 88..155
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..57
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..44
NoneNo IPR availablePANTHERPTHR31221:SF17WRKY TRANSCRIPTION FACTOR 13-RELATEDcoord: 39..162
IPR044810WRKY transcription factor, plantPANTHERPTHR31221WRKY TRANSCRIPTION FACTOR PROTEIN 1-RELATEDcoord: 39..162

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0008623.1Lag0008623.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
molecular_function GO:0003700 DNA-binding transcription factor activity
molecular_function GO:0043565 sequence-specific DNA binding