Lag0008476 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0008476
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRetrovirus-related Pol polyprotein from transposon TNT 1-94
Locationchr9: 22946503 .. 22947362 (-)
RNA-Seq ExpressionLag0008476
SyntenyLag0008476
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTCTTCGATTCACACTGAAGTTGCAACCACTGAAGAAATCGAAAGTTCGGTGACCTCCGTTGTAAATCTAGGTAACAAGATTTCTACAGTCAAGCTAGGAAATGATAATTTCCTGATGTGGAAGGTGCAAATCGAATTTGCCCTAGAAGGCCATGAATTGGAGAATTTTATCTTGGAAGATACTGAACCTCTGCCAAAGCAAATCAAAGCGTCTGATGATTCTTTGAAATCAAATCCAGCCTATGCAAAGTGGAAGAAACAAGATCGACTGATCTCTTCTTGGCTTCTTGGCTCCATGACTGACAGTATCTTGGAGCAAGTTATTCATTGCAAGACGTCCCGTGCCATATGGAAATGCCTCATGCAGATCTTAACTCTCGTAATCGTGCACAAATCATGCGTTTGAAATCTAGGCTTCAAACCATTCAGAAAGGTAACATGACACTCAACGAATACTTCTCGCATATCAAGAGATGTGTTGATGCCCTTGCAGCAATCGAAAAGGTAGTAGATGTCGAAGATCACATTACGTACATTCTGACAGGACTTGGTTCGGAATACGAATCAATGATATCAGTCATCACTGCTCAAACTGATAACATAGGAGTTCAAGAAATGATGGCTTTACTTTTAACACATGAGAATCAGATTGAGAGCAAAGCTAAATCTGTGAATACTGATGGATCTGTTCCTTCAGCAAACTTAATGACACAGAGCTCCCATCCTACAGAATCCGATACTCCGAAACAGAACAATCAATTAAGTGCTCCTTCAAACTTCAACAATCGAGGCAGAGGTGGTGGTAGATCTAATCGCAGAGGGCGTTCTTGGAACAATAACAGAAACAAGCCTTAG

mRNA sequence

ATGGATTCTTCGATTCACACTGAAGTTGCAACCACTGAAGAAATCGAAAGTTCGGTGACCTCCGTTGTAAATCTAGGTAACAAGATTTCTACAGTCAAGCTAGGAAATGATAATTTCCTGATGTGGAAGGTGCAAATCGAATTTGCCCTAGAAGGCCATGAATTGGAGAATTTTATCTTGGAAGATACTGAACCTCTGCCAAAGCAAATCAAAGCGTCTGATGATTCTTTGAAATCAAATCCAGCCTATGCAAAGTGGAAGAAACAAGATCGACTGATCTCTTCTTGGCTTCTTGGCTCCATGACTGACAATCTTAACTCTCGTAATCGTGCACAAATCATGCGTTTGAAATCTAGGCTTCAAACCATTCAGAAAGGTAACATGACACTCAACGAATACTTCTCGCATATCAAGAGATGTGTTGATGCCCTTGCAGCAATCGAAAAGGTAGTAGATGTCGAAGATCACATTACGTACATTCTGACAGGACTTGGTTCGGAATACGAATCAATGATATCAGTCATCACTGCTCAAACTGATAACATAGGAGTTCAAGAAATGATGGCTTTACTTTTAACACATGAGAATCAGATTGAGAGCAAAGCTAAATCTGTGAATACTGATGGATCTGTTCCTTCAGCAAACTTAATGACACAGAGCTCCCATCCTACAGAATCCGATACTCCGAAACAGAACAATCAATTAAGTGCTCCTTCAAACTTCAACAATCGAGGCAGAGGTGGTGGTAGATCTAATCGCAGAGGGCGTTCTTGGAACAATAACAGAAACAAGCCTTAG

Coding sequence (CDS)

ATGGATTCTTCGATTCACACTGAAGTTGCAACCACTGAAGAAATCGAAAGTTCGGTGACCTCCGTTGTAAATCTAGGTAACAAGATTTCTACAGTCAAGCTAGGAAATGATAATTTCCTGATGTGGAAGGTGCAAATCGAATTTGCCCTAGAAGGCCATGAATTGGAGAATTTTATCTTGGAAGATACTGAACCTCTGCCAAAGCAAATCAAAGCGTCTGATGATTCTTTGAAATCAAATCCAGCCTATGCAAAGTGGAAGAAACAAGATCGACTGATCTCTTCTTGGCTTCTTGGCTCCATGACTGACAATCTTAACTCTCGTAATCGTGCACAAATCATGCGTTTGAAATCTAGGCTTCAAACCATTCAGAAAGGTAACATGACACTCAACGAATACTTCTCGCATATCAAGAGATGTGTTGATGCCCTTGCAGCAATCGAAAAGGTAGTAGATGTCGAAGATCACATTACGTACATTCTGACAGGACTTGGTTCGGAATACGAATCAATGATATCAGTCATCACTGCTCAAACTGATAACATAGGAGTTCAAGAAATGATGGCTTTACTTTTAACACATGAGAATCAGATTGAGAGCAAAGCTAAATCTGTGAATACTGATGGATCTGTTCCTTCAGCAAACTTAATGACACAGAGCTCCCATCCTACAGAATCCGATACTCCGAAACAGAACAATCAATTAAGTGCTCCTTCAAACTTCAACAATCGAGGCAGAGGTGGTGGTAGATCTAATCGCAGAGGGCGTTCTTGGAACAATAACAGAAACAAGCCTTAG

Protein sequence

MDSSIHTEVATTEEIESSVTSVVNLGNKISTVKLGNDNFLMWKVQIEFALEGHELENFILEDTEPLPKQIKASDDSLKSNPAYAKWKKQDRLISSWLLGSMTDNLNSRNRAQIMRLKSRLQTIQKGNMTLNEYFSHIKRCVDALAAIEKVVDVEDHITYILTGLGSEYESMISVITAQTDNIGVQEMMALLLTHENQIESKAKSVNTDGSVPSANLMTQSSHPTESDTPKQNNQLSAPSNFNNRGRGGGRSNRRGRSWNNNRNKP
Homology
BLAST of Lag0008476 vs. NCBI nr
Match: KAA0048297.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. makuwa])

HSP 1 Score: 198.4 bits (503), Expect = 8.0e-47
Identity = 131/280 (46.79%), Postives = 174/280 (62.14%), Query Frame = 0

Query: 14  EIESSVTSVVNLGNKISTVKLGNDNFLMWKVQIEFALEGHELENFILEDTEPLPKQIKAS 73
           E  S +  +   GNKIS VKL +D FL+WK QI  ALE ++LENF+  ++EP  K + ++
Sbjct: 14  EASSPINQIFGSGNKISLVKLNDDTFLLWKFQILTALEAYDLENFLESESEPPSKYLIST 73

Query: 74  DDSLKS-----NPAYAKWKKQDRLISSWLLGSMTDNL---------------------NS 133
           + S  S     NPAY  WK+QDRLISSWLLGSM++ +                     +S
Sbjct: 74  ESSSASATGTPNPAYKVWKRQDRLISSWLLGSMSEEILNQMLHCKSAKEIWETLQGIFSS 133

Query: 134 RNRAQIMRLKSRLQTIQKGNMTLNEYFSHIKRCVDALAAIEKVVDVEDHITYILTGLGSE 193
           R  AQ M+ K++L  I+KG+M L EYF  I +CVDALA+I K V  +DHI YIL GLGS+
Sbjct: 134 RYLAQAMQFKNKLHNIKKGSMPLKEYFLKILQCVDALASINKPVSSDDHILYILAGLGSD 193

Query: 194 YESMISVITAQTDNIGVQEMMALLLTHENQIESKAKSVNTDGSVPSANLMTQSSHP-TES 253
           Y+SMISVI+A+TD+  VQE+M+LLLT E+Q ESK  S   + ++PS N++TQ++    ES
Sbjct: 194 YQSMISVISARTDSPSVQEVMSLLLTQESQNESKLIS---ETALPSVNIVTQTTEKGAES 253

Query: 254 DTPKQNNQLSAPSNFNNR-GRGGGRSNRRGRSWNNNRNKP 266
                 N      ++N R GRG GRSNR  R    NRNKP
Sbjct: 254 YIRTNQNNYHNNHSYNQRGGRGNGRSNRGRR---GNRNKP 287

BLAST of Lag0008476 vs. NCBI nr
Match: TYK10642.1 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. makuwa])

HSP 1 Score: 198.4 bits (503), Expect = 8.0e-47
Identity = 131/280 (46.79%), Postives = 174/280 (62.14%), Query Frame = 0

Query: 14  EIESSVTSVVNLGNKISTVKLGNDNFLMWKVQIEFALEGHELENFILEDTEPLPKQIKAS 73
           E  S +  +   GNKIS VKL +D FL+WK QI  ALE ++LENF+  ++EP  K + ++
Sbjct: 14  EASSPINQIFGSGNKISLVKLNDDTFLLWKFQILTALEAYDLENFLESESEPPSKYLIST 73

Query: 74  DDSLKS-----NPAYAKWKKQDRLISSWLLGSMTDNL---------------------NS 133
           + S  S     NPAY  WK+QDRLISSWLLGSM++ +                     +S
Sbjct: 74  ESSSASATGTPNPAYKVWKRQDRLISSWLLGSMSEEILNQMLHCKSAKEIWETLQGIFSS 133

Query: 134 RNRAQIMRLKSRLQTIQKGNMTLNEYFSHIKRCVDALAAIEKVVDVEDHITYILTGLGSE 193
           R  AQ M+ K++L  I+KG+M L EYF  I +CVDALA+I K V  +DHI YIL GLGS+
Sbjct: 134 RYLAQAMQFKNKLHNIKKGSMPLKEYFLKILQCVDALASINKPVSSDDHILYILAGLGSD 193

Query: 194 YESMISVITAQTDNIGVQEMMALLLTHENQIESKAKSVNTDGSVPSANLMTQSSHP-TES 253
           Y+SMISVI+A+TD+  VQE+M+LLLT E+Q ESK  S   + ++PS N++TQ++    ES
Sbjct: 194 YQSMISVISARTDSPSVQEVMSLLLTQESQNESKLIS---ETALPSVNIVTQTTEKGAES 253

Query: 254 DTPKQNNQLSAPSNFNNR-GRGGGRSNRRGRSWNNNRNKP 266
                 N      ++N R GRG GRSNR  R    NRNKP
Sbjct: 254 YIRTNQNNYHNNHSYNQRGGRGNGRSNRGRR---GNRNKP 287

BLAST of Lag0008476 vs. NCBI nr
Match: KAA0053143.1 (keratin, type II cytoskeletal 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 180.3 bits (456), Expect = 2.2e-41
Identity = 104/233 (44.64%), Postives = 149/233 (63.95%), Query Frame = 0

Query: 15  IESSVTSVVNLGNKISTVKLGNDNFLMWKVQIEFALEGHELENFILEDTEPLPKQIKASD 74
           +E++  S+   GNKIS VKL +DNFL+WK QI  ALE ++LENF   + EP  K + ++ 
Sbjct: 10  VENTEVSIFGSGNKISLVKLSDDNFLLWKFQILTALEAYDLENFFESELEPPSKYLTSTG 69

Query: 75  DSLKS-----NPAYAKWKKQDRLISSWLLGSMTDNL---------------------NSR 134
            S  S     NP Y  WK+ +RLIS WLLGSM++ +                     +SR
Sbjct: 70  SSSTSATRTPNPEYKVWKRHNRLISPWLLGSMSEEILNQMVHCKSAKEIWGTLQGIFSSR 129

Query: 135 NRAQIMRLKSRLQTIQKGNMTLNEYFSHIKRCVDALAAIEKVVDVEDHITYILTGLGSEY 194
             AQ M+ K++L  I+KG+M+L EYF  I++CVDALA+I K V  +DHI YIL GLG +Y
Sbjct: 130 YLAQAMQFKNKLHNIKKGSMSLKEYFLKIQQCVDALASINKPVSSDDHILYILVGLGYDY 189

Query: 195 ESMISVITAQTDNIGVQEMMALLLTHENQIESKAKSVNTDGSVPSANLMTQSS 222
           +SMIS+I+A+TD+  +QE+M+LLLT E+Q ESK  S   + ++P   ++TQ++
Sbjct: 190 QSMISIISARTDSPSIQEVMSLLLTQESQNESKLIS---ETALPYVKIVTQTT 239

BLAST of Lag0008476 vs. NCBI nr
Match: XP_022154487.1 (uncharacterized protein LOC111021757 [Momordica charantia])

HSP 1 Score: 163.3 bits (412), Expect = 2.8e-36
Identity = 110/279 (39.43%), Postives = 163/279 (58.42%), Query Frame = 0

Query: 20  TSVVNLGNKISTVKLGNDNFLMWKVQIEFALEGHELENFILEDTEPLPKQI------KAS 79
           +  +N G+K+S V+L +DN L+WK QI  AL+G+ LE++I +  E  P Q       ++S
Sbjct: 19  SKTINPGSKVSIVRLNDDNXLLWKFQIRTALQGNGLESYI-DSNEDTPAQFVQTTEDESS 78

Query: 80  DDSLKSNPAYAKWKKQDRLISSWLLGSMTDNL---------------------NSRNRAQ 139
             SL+ NPAY +W KQD+LIS+WLLGSM +++                      SR  A+
Sbjct: 79  SSSLQQNPAYFEWIKQDKLISAWLLGSMNEDILSQMLDCKSAREIWTVLECMFASRTLAR 138

Query: 140 IMRLKSRLQTIQKGNMTLNEYFSHIKRCVDALAAIEKVVDVEDHITYILTGLGSEYESMI 199
           +M+LK +L+  +KGN++L +YF  IK  VD+LA   K +  EDHI +IL GLG E++++I
Sbjct: 139 VMQLKLKLENFKKGNLSLKDYFLKIKNLVDSLAIAGKKLSTEDHIMHILAGLGPEFDAII 198

Query: 200 SVITAQTDNIGVQEMMALLLTHENQIESKAKSVNTDGSVPSANLMTQSSHPTESDTPKQN 259
           SVITA+     +QE+ +LLL  E + E     +N+DGS+PS NL       T +D+ K+N
Sbjct: 199 SVITARNMPQTLQEVCSLLLQQEGRNERNL--INSDGSLPSVNL-------TLNDSSKKN 258

Query: 260 NQLSA------PSNFNNRGRGGGRSNRRGRSWNNNRNKP 266
           N   +       SN++ RGRG    +   R+W  N NKP
Sbjct: 259 NLHQSKCFNPHQSNYSQRGRGTNNRSSNRRNWTGN-NKP 286

BLAST of Lag0008476 vs. NCBI nr
Match: KAA0067213.1 (keratin, type II cytoskeletal 1-like [Cucumis melo var. makuwa])

HSP 1 Score: 152.1 bits (383), Expect = 6.6e-33
Identity = 89/197 (45.18%), Postives = 119/197 (60.41%), Query Frame = 0

Query: 14  EIESSVTSVVNLGNKISTVKLGNDNFLMWKVQIEFALEGHELENFILEDTEPLPKQIKAS 73
           E  S +  +    NKIS VKL +DNFL+WK QI  ALE ++LENF+  ++EP  K + ++
Sbjct: 14  EASSPINQIFGSSNKISLVKLSDDNFLLWKFQILTALEAYDLENFLESESEPPSKYLIST 73

Query: 74  DDSLKS-----NPAYAKWKKQDRLISSWLLGSMTDNL---------------------NS 133
             S  S     NP Y  WK+QDRLISSWLLGSM++ +                     +S
Sbjct: 74  GSSSASATRTPNPTYKVWKRQDRLISSWLLGSMSEEILNQMLHCKSAKEIWGTLQGIFSS 133

Query: 134 RNRAQIMRLKSRLQTIQKGNMTLNEYFSHIKRCVDALAAIEKVVDVEDHITYILTGLGSE 185
           R  AQ M+ K++L  I+K +M L EYF  I+  VDALA+I K V  +DHI YIL GLGS+
Sbjct: 134 RYLAQAMKFKNKLHNIKKESMPLKEYFLKIQHRVDALASINKPVSSDDHILYILAGLGSD 193

BLAST of Lag0008476 vs. ExPASy Swiss-Prot
Match: Q94HW2 (Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana OX=3702 GN=RE1 PE=2 SV=1)

HSP 1 Score: 81.6 bits (200), Expect = 1.4e-14
Identity = 76/278 (27.34%), Postives = 134/278 (48.20%), Query Frame = 0

Query: 10  ATTEEIESSVTSVVNLGNKISTVKLGNDNFLMWKVQIEFALEGHELENFILEDTEPLPKQ 69
           A  EE+  + TS++N+ N  +  KL + N+LMW  Q+    +G+EL  F L+ +  +P  
Sbjct: 3   AHAEELVLNNTSILNV-NMSNVTKLTSTNYLMWSRQVHALFDGYELAGF-LDGSTTMPPA 62

Query: 70  IKASDDSLKSNPAYAKWKKQDRLISSWLLGSMTDNLN---------------------SR 129
              +D + + NP Y +WK+QD+LI S +LG+++ ++                      + 
Sbjct: 63  TIGTDAAPRVNPDYTRWKRQDKLIYSAVLGAISMSVQPAVSRATTAAQIWETLRKIYANP 122

Query: 130 NRAQIMRLKSRLQTIQKGNMTLNEYFSHIKRCVDALAAIEKVVDVEDHITYILTGLGSEY 189
           +   + +L+++L+   KG  T+++Y   +    D LA + K +D ++ +  +L  L  EY
Sbjct: 123 SYGHVTQLRTQLKQWTKGTKTIDDYMQGLVTRFDQLALLGKPMDHDEQVERVLENLPEEY 182

Query: 190 ESMISVITAQTDNIGVQEMMALLLTHENQIESKAKSVNTDGSVP-SANLMTQSSHPTESD 249
           + +I  I A+     + E+   LL H    ESK  +V++   +P +AN +   SH   + 
Sbjct: 183 KPVIDQIAAKDTPPTLTEIHERLLNH----ESKILAVSSATVIPITANAV---SHRNTTT 242

Query: 250 TPKQNNQLSAPSNFNNRGRGGGRSNRRGRSWNNNRNKP 266
           T   NN              G R+NR     NNN +KP
Sbjct: 243 TNNNNN--------------GNRNNRYDNRNNNNNSKP 257

BLAST of Lag0008476 vs. ExPASy Swiss-Prot
Match: Q9ZT94 (Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana OX=3702 GN=RE2 PE=4 SV=1)

HSP 1 Score: 72.8 bits (177), Expect = 6.6e-12
Identity = 70/257 (27.24%), Postives = 128/257 (49.81%), Query Frame = 0

Query: 13  EEIESSVTSVVNLGNKISTVKLGNDNFLMWKVQIEFALEGHELENFILEDTEPLPKQIKA 72
           EEI    T+++N+ N  +  KL + N+LMW  Q+    +G+EL  F L+ + P+P     
Sbjct: 6   EEIVLVNTNILNV-NMSNVTKLTSTNYLMWSRQVHALFDGYELAGF-LDGSTPMPPATIG 65

Query: 73  SDDSLKSNPAYAKWKKQDRLISSWLLGSMTDNL-----NSRNRAQIMRLKSRLQTIQKGN 132
           +D   + NP Y +W++QD+LI S +LG+++ ++      +   AQI       +T++K  
Sbjct: 66  TDAVPRVNPDYTRWRRQDKLIYSAILGAISMSVQPAVSRATTAAQIW------ETLRK-- 125

Query: 133 MTLNEYFSHIKRC-----VDALAAIEKVVDVEDHITYILTGLGSEYESMISVITAQTDNI 192
           +  N  + H+ +       D LA + K +D ++ +  +L  L  +Y+ +I  I A+    
Sbjct: 126 IYANPSYGHVTQLRFITRFDQLALLGKPMDHDEQVERVLENLPDDYKPVIDQIAAKDTPP 185

Query: 193 GVQEMMALLLTHENQIESKAKSVNTDGSVP-SANLMTQSSHPTESDTPKQNNQLSAPSNF 252
            + E+   L+      ESK  ++N+   VP +AN++T  +    ++T +  N      N+
Sbjct: 186 SLTEIHERLINR----ESKLLALNSAEVVPITANVVTHRN----TNTNRNQNNRGDNRNY 237

Query: 253 NNRGRGGGRSNRRGRSW 259
           NN       +N R  SW
Sbjct: 246 NN-------NNNRSNSW 237

BLAST of Lag0008476 vs. ExPASy TrEMBL
Match: A0A5A7U233 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold264G00060 PE=4 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 3.9e-47
Identity = 131/280 (46.79%), Postives = 174/280 (62.14%), Query Frame = 0

Query: 14  EIESSVTSVVNLGNKISTVKLGNDNFLMWKVQIEFALEGHELENFILEDTEPLPKQIKAS 73
           E  S +  +   GNKIS VKL +D FL+WK QI  ALE ++LENF+  ++EP  K + ++
Sbjct: 14  EASSPINQIFGSGNKISLVKLNDDTFLLWKFQILTALEAYDLENFLESESEPPSKYLIST 73

Query: 74  DDSLKS-----NPAYAKWKKQDRLISSWLLGSMTDNL---------------------NS 133
           + S  S     NPAY  WK+QDRLISSWLLGSM++ +                     +S
Sbjct: 74  ESSSASATGTPNPAYKVWKRQDRLISSWLLGSMSEEILNQMLHCKSAKEIWETLQGIFSS 133

Query: 134 RNRAQIMRLKSRLQTIQKGNMTLNEYFSHIKRCVDALAAIEKVVDVEDHITYILTGLGSE 193
           R  AQ M+ K++L  I+KG+M L EYF  I +CVDALA+I K V  +DHI YIL GLGS+
Sbjct: 134 RYLAQAMQFKNKLHNIKKGSMPLKEYFLKILQCVDALASINKPVSSDDHILYILAGLGSD 193

Query: 194 YESMISVITAQTDNIGVQEMMALLLTHENQIESKAKSVNTDGSVPSANLMTQSSHP-TES 253
           Y+SMISVI+A+TD+  VQE+M+LLLT E+Q ESK  S   + ++PS N++TQ++    ES
Sbjct: 194 YQSMISVISARTDSPSVQEVMSLLLTQESQNESKLIS---ETALPSVNIVTQTTEKGAES 253

Query: 254 DTPKQNNQLSAPSNFNNR-GRGGGRSNRRGRSWNNNRNKP 266
                 N      ++N R GRG GRSNR  R    NRNKP
Sbjct: 254 YIRTNQNNYHNNHSYNQRGGRGNGRSNRGRR---GNRNKP 287

BLAST of Lag0008476 vs. ExPASy TrEMBL
Match: A0A5D3CH97 (Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold25G00040 PE=4 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 3.9e-47
Identity = 131/280 (46.79%), Postives = 174/280 (62.14%), Query Frame = 0

Query: 14  EIESSVTSVVNLGNKISTVKLGNDNFLMWKVQIEFALEGHELENFILEDTEPLPKQIKAS 73
           E  S +  +   GNKIS VKL +D FL+WK QI  ALE ++LENF+  ++EP  K + ++
Sbjct: 14  EASSPINQIFGSGNKISLVKLNDDTFLLWKFQILTALEAYDLENFLESESEPPSKYLIST 73

Query: 74  DDSLKS-----NPAYAKWKKQDRLISSWLLGSMTDNL---------------------NS 133
           + S  S     NPAY  WK+QDRLISSWLLGSM++ +                     +S
Sbjct: 74  ESSSASATGTPNPAYKVWKRQDRLISSWLLGSMSEEILNQMLHCKSAKEIWETLQGIFSS 133

Query: 134 RNRAQIMRLKSRLQTIQKGNMTLNEYFSHIKRCVDALAAIEKVVDVEDHITYILTGLGSE 193
           R  AQ M+ K++L  I+KG+M L EYF  I +CVDALA+I K V  +DHI YIL GLGS+
Sbjct: 134 RYLAQAMQFKNKLHNIKKGSMPLKEYFLKILQCVDALASINKPVSSDDHILYILAGLGSD 193

Query: 194 YESMISVITAQTDNIGVQEMMALLLTHENQIESKAKSVNTDGSVPSANLMTQSSHP-TES 253
           Y+SMISVI+A+TD+  VQE+M+LLLT E+Q ESK  S   + ++PS N++TQ++    ES
Sbjct: 194 YQSMISVISARTDSPSVQEVMSLLLTQESQNESKLIS---ETALPSVNIVTQTTEKGAES 253

Query: 254 DTPKQNNQLSAPSNFNNR-GRGGGRSNRRGRSWNNNRNKP 266
                 N      ++N R GRG GRSNR  R    NRNKP
Sbjct: 254 YIRTNQNNYHNNHSYNQRGGRGNGRSNRGRR---GNRNKP 287

BLAST of Lag0008476 vs. ExPASy TrEMBL
Match: A0A5A7UB21 (Keratin, type II cytoskeletal 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1486G00150 PE=4 SV=1)

HSP 1 Score: 180.3 bits (456), Expect = 1.1e-41
Identity = 104/233 (44.64%), Postives = 149/233 (63.95%), Query Frame = 0

Query: 15  IESSVTSVVNLGNKISTVKLGNDNFLMWKVQIEFALEGHELENFILEDTEPLPKQIKASD 74
           +E++  S+   GNKIS VKL +DNFL+WK QI  ALE ++LENF   + EP  K + ++ 
Sbjct: 10  VENTEVSIFGSGNKISLVKLSDDNFLLWKFQILTALEAYDLENFFESELEPPSKYLTSTG 69

Query: 75  DSLKS-----NPAYAKWKKQDRLISSWLLGSMTDNL---------------------NSR 134
            S  S     NP Y  WK+ +RLIS WLLGSM++ +                     +SR
Sbjct: 70  SSSTSATRTPNPEYKVWKRHNRLISPWLLGSMSEEILNQMVHCKSAKEIWGTLQGIFSSR 129

Query: 135 NRAQIMRLKSRLQTIQKGNMTLNEYFSHIKRCVDALAAIEKVVDVEDHITYILTGLGSEY 194
             AQ M+ K++L  I+KG+M+L EYF  I++CVDALA+I K V  +DHI YIL GLG +Y
Sbjct: 130 YLAQAMQFKNKLHNIKKGSMSLKEYFLKIQQCVDALASINKPVSSDDHILYILVGLGYDY 189

Query: 195 ESMISVITAQTDNIGVQEMMALLLTHENQIESKAKSVNTDGSVPSANLMTQSS 222
           +SMIS+I+A+TD+  +QE+M+LLLT E+Q ESK  S   + ++P   ++TQ++
Sbjct: 190 QSMISIISARTDSPSIQEVMSLLLTQESQNESKLIS---ETALPYVKIVTQTT 239

BLAST of Lag0008476 vs. ExPASy TrEMBL
Match: A0A6J1DLT9 (uncharacterized protein LOC111021757 OS=Momordica charantia OX=3673 GN=LOC111021757 PE=4 SV=1)

HSP 1 Score: 163.3 bits (412), Expect = 1.4e-36
Identity = 110/279 (39.43%), Postives = 163/279 (58.42%), Query Frame = 0

Query: 20  TSVVNLGNKISTVKLGNDNFLMWKVQIEFALEGHELENFILEDTEPLPKQI------KAS 79
           +  +N G+K+S V+L +DN L+WK QI  AL+G+ LE++I +  E  P Q       ++S
Sbjct: 19  SKTINPGSKVSIVRLNDDNXLLWKFQIRTALQGNGLESYI-DSNEDTPAQFVQTTEDESS 78

Query: 80  DDSLKSNPAYAKWKKQDRLISSWLLGSMTDNL---------------------NSRNRAQ 139
             SL+ NPAY +W KQD+LIS+WLLGSM +++                      SR  A+
Sbjct: 79  SSSLQQNPAYFEWIKQDKLISAWLLGSMNEDILSQMLDCKSAREIWTVLECMFASRTLAR 138

Query: 140 IMRLKSRLQTIQKGNMTLNEYFSHIKRCVDALAAIEKVVDVEDHITYILTGLGSEYESMI 199
           +M+LK +L+  +KGN++L +YF  IK  VD+LA   K +  EDHI +IL GLG E++++I
Sbjct: 139 VMQLKLKLENFKKGNLSLKDYFLKIKNLVDSLAIAGKKLSTEDHIMHILAGLGPEFDAII 198

Query: 200 SVITAQTDNIGVQEMMALLLTHENQIESKAKSVNTDGSVPSANLMTQSSHPTESDTPKQN 259
           SVITA+     +QE+ +LLL  E + E     +N+DGS+PS NL       T +D+ K+N
Sbjct: 199 SVITARNMPQTLQEVCSLLLQQEGRNERNL--INSDGSLPSVNL-------TLNDSSKKN 258

Query: 260 NQLSA------PSNFNNRGRGGGRSNRRGRSWNNNRNKP 266
           N   +       SN++ RGRG    +   R+W  N NKP
Sbjct: 259 NLHQSKCFNPHQSNYSQRGRGTNNRSSNRRNWTGN-NKP 286

BLAST of Lag0008476 vs. ExPASy TrEMBL
Match: A0A5A7VGJ8 (Keratin, type II cytoskeletal 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold418G00160 PE=4 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 3.2e-33
Identity = 89/197 (45.18%), Postives = 119/197 (60.41%), Query Frame = 0

Query: 14  EIESSVTSVVNLGNKISTVKLGNDNFLMWKVQIEFALEGHELENFILEDTEPLPKQIKAS 73
           E  S +  +    NKIS VKL +DNFL+WK QI  ALE ++LENF+  ++EP  K + ++
Sbjct: 14  EASSPINQIFGSSNKISLVKLSDDNFLLWKFQILTALEAYDLENFLESESEPPSKYLIST 73

Query: 74  DDSLKS-----NPAYAKWKKQDRLISSWLLGSMTDNL---------------------NS 133
             S  S     NP Y  WK+QDRLISSWLLGSM++ +                     +S
Sbjct: 74  GSSSASATRTPNPTYKVWKRQDRLISSWLLGSMSEEILNQMLHCKSAKEIWGTLQGIFSS 133

Query: 134 RNRAQIMRLKSRLQTIQKGNMTLNEYFSHIKRCVDALAAIEKVVDVEDHITYILTGLGSE 185
           R  AQ M+ K++L  I+K +M L EYF  I+  VDALA+I K V  +DHI YIL GLGS+
Sbjct: 134 RYLAQAMKFKNKLHNIKKESMPLKEYFLKIQHRVDALASINKPVSSDDHILYILAGLGSD 193

BLAST of Lag0008476 vs. TAIR 10
Match: AT5G48050.1 (CONTAINS InterPro DOMAIN/s: Retrotransposon gag protein (InterPro:IPR005162); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G34070.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 51.6 bits (122), Expect = 1.1e-06
Identity = 46/212 (21.70%), Postives = 98/212 (46.23%), Query Frame = 0

Query: 85  KWKKQDRLISSWLLGSMTDNL----------------------NSRNRAQIMRLKSRLQT 144
           +WK++D L+  W+ G++TD+L                           A+ ++ ++ L+T
Sbjct: 66  RWKERDGLVKMWIYGTITDSLLDTIIKVGCTARDLWLSLENLFRDNKEARALQFENELRT 125

Query: 145 IQKGNMTLNEYFSHIKRCVDALAAIEKVVDVEDHITYILTGLGSEYESMISVITAQTDNI 204
               +++++EY   +K   D L  ++  +     + ++L GL  +Y+ +++VI  ++   
Sbjct: 126 TTIDDLSVHEYCQKLKSLSDLLTNVDSPISDRVLVMHLLNGLTEKYDYILNVIKHKSPFP 185

Query: 205 GVQEMMALLLTHENQIESKAKSVNTDGSVPSANLMTQSSHPTESD----TPKQNNQLSAP 264
              E  ++LL  E+++ +K+KS            ++ ++HP+ S+     P+Q  +    
Sbjct: 186 SFTEARSMLLMEESRLSNKSKS-----------SLSHTNHPSLSNVLFTVPRQQERYPQE 245

BLAST of Lag0008476 vs. TAIR 10
Match: AT1G34070.1 (CONTAINS InterPro DOMAIN/s: Retrotransposon gag protein (InterPro:IPR005162); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G48050.1); Has 648 Blast hits to 647 proteins in 29 species: Archae - 0; Bacteria - 0; Metazoa - 16; Fungi - 25; Plants - 607; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 45.1 bits (105), Expect = 1.1e-04
Identity = 46/202 (22.77%), Postives = 88/202 (43.56%), Query Frame = 0

Query: 77  LKSNPAYAKWKKQDRLISSWLLGSMT----------------------DNLNSRNRAQIM 136
           L +N     W+K+D ++   L G++T                      +   +   A+ +
Sbjct: 56  LPTNANDVNWQKRDGIVKLSLYGTLTPKQFQGSFVTSSTSRDIWLRIKNQFRNNKDARAL 115

Query: 137 RLKSRLQTIQKGNMTLNEYFSHIKRCVDALAAIEKVVDVEDHITYILTGLGSEYESMISV 196
           RL S L+T   G+M + +Y+  +K+  D+L  ++  V   + + Y+L GL  +++++I+V
Sbjct: 116 RLDSELRTKDIGDMRVADYYRKMKKLADSLRNVDVPVTDRNLVMYVLNGLNPKFDNIINV 175

Query: 197 ITAQTDNIGVQEMMALLLTHENQIESKAKSVNTDGSVPSANLMTQSSHPTESDTPKQNNQ 256
           I  +       +   +L   E++++   K   T     S++ +   S       P  N Q
Sbjct: 176 IKHRQPFPSFDDAATMLQEEEDRLKRAIKPNPTHVDHSSSSTVLACSEA----PPVTNFQ 235

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0048297.18.0e-4746.79Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. m... [more]
TYK10642.18.0e-4746.79Retrovirus-related Pol polyprotein from transposon TNT 1-94 [Cucumis melo var. m... [more]
KAA0053143.12.2e-4144.64keratin, type II cytoskeletal 1-like [Cucumis melo var. makuwa][more]
XP_022154487.12.8e-3639.43uncharacterized protein LOC111021757 [Momordica charantia][more]
KAA0067213.16.6e-3345.18keratin, type II cytoskeletal 1-like [Cucumis melo var. makuwa][more]
Match NameE-valueIdentityDescription
Q94HW21.4e-1427.34Retrovirus-related Pol polyprotein from transposon RE1 OS=Arabidopsis thaliana O... [more]
Q9ZT946.6e-1227.24Retrovirus-related Pol polyprotein from transposon RE2 OS=Arabidopsis thaliana O... [more]
Match NameE-valueIdentityDescription
A0A5A7U2333.9e-4746.79Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var.... [more]
A0A5D3CH973.9e-4746.79Retrovirus-related Pol polyprotein from transposon TNT 1-94 OS=Cucumis melo var.... [more]
A0A5A7UB211.1e-4144.64Keratin, type II cytoskeletal 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A6J1DLT91.4e-3639.43uncharacterized protein LOC111021757 OS=Momordica charantia OX=3673 GN=LOC111021... [more]
A0A5A7VGJ83.2e-3345.18Keratin, type II cytoskeletal 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
Match NameE-valueIdentityDescription
AT5G48050.11.1e-0621.70CONTAINS InterPro DOMAIN/s: Retrotransposon gag protein (InterPro:IPR005162); BE... [more]
AT1G34070.11.1e-0422.77CONTAINS InterPro DOMAIN/s: Retrotransposon gag protein (InterPro:IPR005162); BE... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF14223Retrotran_gag_2coord: 108..197
e-value: 2.4E-9
score: 37.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 202..265
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 202..256
NoneNo IPR availablePANTHERPTHR34222FAMILY NOT NAMEDcoord: 30..254
NoneNo IPR availablePANTHERPTHR34222:SF48SUBFAMILY NOT NAMEDcoord: 30..254

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0008476.1Lag0008476.1mRNA