Lag0008221 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0008221 vs. NCBI nr
Match: XP_022158222.1 (uncharacterized membrane protein At3g27390-like isoform X1 [Momordica charantia] >XP_022158223.1 uncharacterized membrane protein At3g27390-like isoform X1 [Momordica charantia])

HSP 1 Score: 759.2 bits (1959), Expect = 2.0e-215
Identity = 363/434 (83.64%), Postives = 399/434 (91.94%), Query Frame = 0

Query: 1   MFRPFTKCLMILVLPVPIALWIVIGVIGSVVMGIGYAFVWPFMETFRAISREGVPVYMRL 60
           MF PFTK L+IL+LP+PIA+W VIGV+GSV+MGIGY FVWPFMETFRAISREGVP+YMRL
Sbjct: 71  MFGPFTKFLLILILPIPIAIWTVIGVVGSVIMGIGYGFVWPFMETFRAISREGVPIYMRL 130

Query: 61  IRCFTDGTWSNVWGACTTVRDFADLSFHSYFSIMEGMVEWKGEESIDIKVYEIAGCILVA 120
           IRCFTDGTWSNVWGACT VRDFAD SFHSYFSIM+ MVEWKGEE +DIKVYE+AGC+LVA
Sbjct: 131 IRCFTDGTWSNVWGACTIVRDFADFSFHSYFSIMDQMVEWKGEEPVDIKVYEVAGCVLVA 190

Query: 121 IVGILIDFPIITLIVLYKVPIMLVKGWHRLIQDLIGREGPFLETVCVPFAGLWILLWPAV 180
           IVGI++DFPII L+VLYK PIMLVKGWHRLIQDL+GREGPFLETVCVPFAGLWILLWP V
Sbjct: 191 IVGIVVDFPIIPLLVLYKAPIMLVKGWHRLIQDLVGREGPFLETVCVPFAGLWILLWPFV 250

Query: 181 VLIAAFAGITSSIGYGCYAAVVAYQESSLKRGLLYVVASISLFDEYTNDLLYLREGSCFP 240
           VL+A+FAGITS IG+GCYAAVVA+QE+SLKRGLLYV+ASISLFDEYTNDLLYLREGSCFP
Sbjct: 251 VLLASFAGITSIIGFGCYAAVVAFQENSLKRGLLYVIASISLFDEYTNDLLYLREGSCFP 310

Query: 241 RPKYREKMDTSSSMLPVQEFRKHLEAIHTGEPLIRNMAEHKSVVKAVVILDGFYKACEDI 300
           RPKYREKMD+SS ++P +   +HL+AIHT EPLI   AE KS VKAVVI D F+KAC DI
Sbjct: 311 RPKYREKMDSSSLLVPGKGLHEHLQAIHTREPLIMTTAEQKSAVKAVVIWDSFFKACVDI 370

Query: 301 GKDLIRDEAIGLADLEAWRHAENKIVNIGIPSHAFLQCFLRSIKSGSTGFLMRDNIEITS 360
           GK+LIRDEAIGLADLEAWRHA+NKIVN+GIPSH FLQCFLRSIK GS GFLMRD++EIT 
Sbjct: 371 GKELIRDEAIGLADLEAWRHAKNKIVNVGIPSHVFLQCFLRSIKRGSVGFLMRDDVEITH 430

Query: 361 VNRPEGRIFDWLYEPMSIMKEQIRRLNLQESEELYLYKLCLYSGDMTRVESWHNGGVPPP 420
           VNRPEGRIFDWLYEPMSIMKEQIRRLNLQE+E+LYLYK CLYSGD  R+ESW NGGVP P
Sbjct: 431 VNRPEGRIFDWLYEPMSIMKEQIRRLNLQENEQLYLYKFCLYSGDTKRIESWENGGVPSP 490

Query: 421 DEIRRAQLEGISRR 435
           DEIRRAQLEGISRR
Sbjct: 491 DEIRRAQLEGISRR 504

BLAST of Lag0008221 vs. NCBI nr
Match: XP_022158226.1 (uncharacterized membrane protein At3g27390-like isoform X1 [Momordica charantia] >XP_022158227.1 uncharacterized membrane protein At3g27390-like isoform X1 [Momordica charantia])

HSP 1 Score: 724.2 bits (1868), Expect = 7.1e-205
Identity = 351/435 (80.69%), Postives = 386/435 (88.74%), Query Frame = 0

Query: 1   MFRPFTKCLMILVLPVPIALWIVIGVIGSVVMGIGYAFVWPFMETFRAISREGVPVYMRL 60
           MF PF KCL+IL+LP+PIA+W VIGV+GSV+MGIGY FVWP MET RAISREG+P++MRL
Sbjct: 71  MFGPFMKCLLILILPIPIAVWTVIGVVGSVIMGIGYGFVWPVMETLRAISREGIPIHMRL 130

Query: 61  IRCFTDGTWSNVWGACTTVRDFADLSFHSYFSIMEGMVEWKGEESIDIKVYEIAGCILVA 120
           IRCFTDGTWSNVWGACT VRDFAD SFHSYFSIM+GMVEW+GEE IDIKV+E+A CILVA
Sbjct: 131 IRCFTDGTWSNVWGACTIVRDFADFSFHSYFSIMDGMVEWQGEEQIDIKVHELASCILVA 190

Query: 121 IVGILIDFPIITLIVLYKVPIMLVKGWHRLIQDLIGREGPFLETVCVPFAGLWILLWPAV 180
           +VGI++DFPIITLIVLYK PIML KGWHRLIQDLIGREGPFLETVCVPFAGLWILLWP  
Sbjct: 191 LVGIMVDFPIITLIVLYKAPIMLFKGWHRLIQDLIGREGPFLETVCVPFAGLWILLWPVA 250

Query: 181 VLIAAFAGITSSIGYGCYAAVVAYQESSLKRGLLYVVASISLFDEYTNDLLYLREGSCFP 240
           V++A FAGI SSIG G Y  VV YQESSLKRGLLYV+ASISLFDEYTNDLLYLREGSCFP
Sbjct: 251 VIMATFAGIASSIGLGLYGVVVTYQESSLKRGLLYVIASISLFDEYTNDLLYLREGSCFP 310

Query: 241 RPKYREKMD-TSSSMLPVQEFRKHLEAIHTGEPLIRNMAEHKSVVKAVVILDGFYKACED 300
           RPKY EKMD +SSS++PV+   +HLE I T EPL+   AE KS VKAVVI D F+KACE+
Sbjct: 311 RPKYSEKMDYSSSSLVPVKGLHEHLERIRTSEPLM-TTAEQKSAVKAVVIWDSFFKACEE 370

Query: 301 IGKDLIRDEAIGLADLEAWRHAENKIVNIGIPSHAFLQCFLRSIKSGSTGFLMRDNIEIT 360
           IGK+LIRDEAIG+ADLEAWR A NKI+++GI SHAFLQCFLRSIKSGS GFLM D I IT
Sbjct: 371 IGKELIRDEAIGVADLEAWRQARNKIIHVGISSHAFLQCFLRSIKSGSAGFLMSDKIVIT 430

Query: 361 SVNRPEGRIFDWLYEPMSIMKEQIRRLNLQESEELYLYKLCLYSGDMTRVESWHNGGVPP 420
           SVNRPEGRIFDWLYEP+SIMKEQIR LNLQESEELYLYK CLYSGD  R+ESW NGG+PP
Sbjct: 431 SVNRPEGRIFDWLYEPLSIMKEQIRCLNLQESEELYLYKFCLYSGDTARIESWENGGIPP 490

Query: 421 PDEIRRAQLEGISRR 435
           PDEIRRAQLEGI RR
Sbjct: 491 PDEIRRAQLEGIGRR 504

BLAST of Lag0008221 vs. NCBI nr
Match: XP_022158225.1 (uncharacterized membrane protein At3g27390-like isoform X3 [Momordica charantia])

HSP 1 Score: 716.8 bits (1849), Expect = 1.1e-202
Identity = 341/403 (84.62%), Postives = 371/403 (92.06%), Query Frame = 0

Query: 32  MGIGYAFVWPFMETFRAISREGVPVYMRLIRCFTDGTWSNVWGACTTVRDFADLSFHSYF 91
           MGIGY FVWPFMETFRAISREGVP+YMRLIRCFTDGTWSNVWGACT VRDFAD SFHSYF
Sbjct: 1   MGIGYGFVWPFMETFRAISREGVPIYMRLIRCFTDGTWSNVWGACTIVRDFADFSFHSYF 60

Query: 92  SIMEGMVEWKGEESIDIKVYEIAGCILVAIVGILIDFPIITLIVLYKVPIMLVKGWHRLI 151
           SIM+ MVEWKGEE +DIKVYE+AGC+LVAIVGI++DFPII L+VLYK PIMLVKGWHRLI
Sbjct: 61  SIMDQMVEWKGEEPVDIKVYEVAGCVLVAIVGIVVDFPIIPLLVLYKAPIMLVKGWHRLI 120

Query: 152 QDLIGREGPFLETVCVPFAGLWILLWPAVVLIAAFAGITSSIGYGCYAAVVAYQESSLKR 211
           QDL+GREGPFLETVCVPFAGLWILLWP VVL+A+FAGITS IG+GCYAAVVA+QE+SLKR
Sbjct: 121 QDLVGREGPFLETVCVPFAGLWILLWPFVVLLASFAGITSIIGFGCYAAVVAFQENSLKR 180

Query: 212 GLLYVVASISLFDEYTNDLLYLREGSCFPRPKYREKMDTSSSMLPVQEFRKHLEAIHTGE 271
           GLLYV+ASISLFDEYTNDLLYLREGSCFPRPKYREKMD+SS ++P +   +HL+AIHT E
Sbjct: 181 GLLYVIASISLFDEYTNDLLYLREGSCFPRPKYREKMDSSSLLVPGKGLHEHLQAIHTRE 240

Query: 272 PLIRNMAEHKSVVKAVVILDGFYKACEDIGKDLIRDEAIGLADLEAWRHAENKIVNIGIP 331
           PLI   AE KS VKAVVI D F+KAC DIGK+LIRDEAIGLADLEAWRHA+NKIVN+GIP
Sbjct: 241 PLIMTTAEQKSAVKAVVIWDSFFKACVDIGKELIRDEAIGLADLEAWRHAKNKIVNVGIP 300

Query: 332 SHAFLQCFLRSIKSGSTGFLMRDNIEITSVNRPEGRIFDWLYEPMSIMKEQIRRLNLQES 391
           SH FLQCFLRSIK GS GFLMRD++EIT VNRPEGRIFDWLYEPMSIMKEQIRRLNLQE+
Sbjct: 301 SHVFLQCFLRSIKRGSVGFLMRDDVEITHVNRPEGRIFDWLYEPMSIMKEQIRRLNLQEN 360

Query: 392 EELYLYKLCLYSGDMTRVESWHNGGVPPPDEIRRAQLEGISRR 435
           E+LYLYK CLYSGD  R+ESW NGGVP PDEIRRAQLEGISRR
Sbjct: 361 EQLYLYKFCLYSGDTKRIESWENGGVPSPDEIRRAQLEGISRR 403

BLAST of Lag0008221 vs. NCBI nr
Match: XP_022158228.1 (uncharacterized membrane protein At3g27390-like isoform X2 [Momordica charantia])

HSP 1 Score: 680.2 bits (1754), Expect = 1.2e-191
Identity = 329/404 (81.44%), Postives = 358/404 (88.61%), Query Frame = 0

Query: 32  MGIGYAFVWPFMETFRAISREGVPVYMRLIRCFTDGTWSNVWGACTTVRDFADLSFHSYF 91
           MGIGY FVWP MET RAISREG+P++MRLIRCFTDGTWSNVWGACT VRDFAD SFHSYF
Sbjct: 1   MGIGYGFVWPVMETLRAISREGIPIHMRLIRCFTDGTWSNVWGACTIVRDFADFSFHSYF 60

Query: 92  SIMEGMVEWKGEESIDIKVYEIAGCILVAIVGILIDFPIITLIVLYKVPIMLVKGWHRLI 151
           SIM+GMVEW+GEE IDIKV+E+A CILVA+VGI++DFPIITLIVLYK PIML KGWHRLI
Sbjct: 61  SIMDGMVEWQGEEQIDIKVHELASCILVALVGIMVDFPIITLIVLYKAPIMLFKGWHRLI 120

Query: 152 QDLIGREGPFLETVCVPFAGLWILLWPAVVLIAAFAGITSSIGYGCYAAVVAYQESSLKR 211
           QDLIGREGPFLETVCVPFAGLWILLWP  V++A FAGI SSIG G Y  VV YQESSLKR
Sbjct: 121 QDLIGREGPFLETVCVPFAGLWILLWPVAVIMATFAGIASSIGLGLYGVVVTYQESSLKR 180

Query: 212 GLLYVVASISLFDEYTNDLLYLREGSCFPRPKYREKMD-TSSSMLPVQEFRKHLEAIHTG 271
           GLLYV+ASISLFDEYTNDLLYLREGSCFPRPKY EKMD +SSS++PV+   +HLE I T 
Sbjct: 181 GLLYVIASISLFDEYTNDLLYLREGSCFPRPKYSEKMDYSSSSLVPVKGLHEHLERIRTS 240

Query: 272 EPLIRNMAEHKSVVKAVVILDGFYKACEDIGKDLIRDEAIGLADLEAWRHAENKIVNIGI 331
           EPL+   AE KS VKAVVI D F+KACE+IGK+LIRDEAIG+ADLEAWR A NKI+++GI
Sbjct: 241 EPLM-TTAEQKSAVKAVVIWDSFFKACEEIGKELIRDEAIGVADLEAWRQARNKIIHVGI 300

Query: 332 PSHAFLQCFLRSIKSGSTGFLMRDNIEITSVNRPEGRIFDWLYEPMSIMKEQIRRLNLQE 391
            SHAFLQCFLRSIKSGS GFLM D I ITSVNRPEGRIFDWLYEP+SIMKEQIR LNLQE
Sbjct: 301 SSHAFLQCFLRSIKSGSAGFLMSDKIVITSVNRPEGRIFDWLYEPLSIMKEQIRCLNLQE 360

Query: 392 SEELYLYKLCLYSGDMTRVESWHNGGVPPPDEIRRAQLEGISRR 435
           SEELYLYK CLYSGD  R+ESW NGG+PPPDEIRRAQLEGI RR
Sbjct: 361 SEELYLYKFCLYSGDTARIESWENGGIPPPDEIRRAQLEGIGRR 403

BLAST of Lag0008221 vs. NCBI nr
Match: KAF3952950.1 (hypothetical protein CMV_021554 [Castanea mollissima])

HSP 1 Score: 670.2 bits (1728), Expect = 1.2e-188
Identity = 316/433 (72.98%), Postives = 375/433 (86.61%), Query Frame = 0

Query: 2   FRPFTKCLMILVLPVPIALWIVIGVIGSVVMGIGYAFVWPFMETFRAISREGVPVYMRLI 61
           F PF KCL+IL++P+PIALW V+GV+GS +MGIGY +VWP +ETFRAIS EGVP++M+L 
Sbjct: 65  FGPFMKCLLILIVPIPIALWTVVGVVGSAIMGIGYGYVWPVLETFRAISIEGVPLHMKLF 124

Query: 62  RCFTDGTWSNVWGACTTVRDFADLSFHSYFSIMEGMVEWKGEESIDIKVYEIAGCILVAI 121
           RCFTDGTWSNVWGACT V DFAD SFHSYFS+M+G++E KGEE I++KV +I GCI+ AI
Sbjct: 125 RCFTDGTWSNVWGACTIVGDFADFSFHSYFSVMDGLLESKGEEPIELKVTQIPGCIVAAI 184

Query: 122 VGILIDFPIITLIVLYKVPIMLVKGWHRLIQDLIGREGPFLETVCVPFAGLWILLWPAVV 181
           +GIL+D P+ITLIVLYK PI+L KGWHRLIQDL+GREGPFLETVCVPFAG+WILLWP VV
Sbjct: 185 LGILVDVPLITLIVLYKTPILLFKGWHRLIQDLVGREGPFLETVCVPFAGIWILLWPIVV 244

Query: 182 LIAAFAGITSSIGYGCYAAVVAYQESSLKRGLLYVVASISLFDEYTNDLLYLREGSCFPR 241
           L+A   G  SS+G+GCYAAVVAYQE+S KRGLLYV+AS+S+FDEYTNDLLYL+EGSCFPR
Sbjct: 245 LMATLLGTLSSLGFGCYAAVVAYQENSTKRGLLYVIASLSIFDEYTNDLLYLQEGSCFPR 304

Query: 242 PKYREKMDTSSSMLPVQEFRKHLEAIHTGEPLIRNMAEHKSVVKAVVILDGFYKACEDIG 301
           P+YRE++ +SSS+LPV+   + +EAI T EPLIR     K+ +KA VI D F++ACEDIG
Sbjct: 305 PRYREQISSSSSLLPVKGLHEQVEAIRTNEPLIRT-PSIKNTLKAAVIWDSFFQACEDIG 364

Query: 302 KDLIRDEAIGLADLEAWRHAENKIVNIGIPSHAFLQCFLRSIKSGSTGFLMRDNIEITSV 361
           K+LIRD AIGL DL+AWRH++NKIVNIGIP++ FL CFLRSIKSGS GFLMR NIEITSV
Sbjct: 365 KELIRDGAIGLQDLDAWRHSKNKIVNIGIPAYVFLDCFLRSIKSGSIGFLMRVNIEITSV 424

Query: 362 NRPEGRIFDWLYEPMSIMKEQIRRLNLQESEELYLYKLCLYSGDMTRVESWHNGGVPPPD 421
           NRPEGR FDWLYEPM I+K+QIR LNLQE+EELY YK CLY G + R+ESW NGG+PP D
Sbjct: 425 NRPEGRAFDWLYEPMCIIKDQIRSLNLQETEELYFYKHCLYGGGVVRIESWQNGGIPPHD 484

Query: 422 EIRRAQLEGISRR 435
           EI+RAQLEGISRR
Sbjct: 485 EIKRAQLEGISRR 496

BLAST of Lag0008221 vs. ExPASy Swiss-Prot
Match: Q8GUM4 (Uncharacterized membrane protein At3g27390 OS=Arabidopsis thaliana OX=3702 GN=At3g27390 PE=1 SV=2)

HSP 1 Score: 327.4 bits (838), Expect = 2.5e-88
Identity = 184/438 (42.01%), Postives = 268/438 (61.19%), Query Frame = 0

Query: 4   PFTKCLMILVLPVPIALWIVIGVIGSVVMGIGYAFVWPFMETFRAISREGVPVYMRLIRC 63
           P  K  + L LP  I LW ++G++GSV+ G  Y F  P   TF A+  EG P   +   C
Sbjct: 74  PILKIFLCLCLPAAIILWPIVGILGSVLGGALYGFFSPIFATFDAVG-EGKP--YQFFHC 133

Query: 64  FTDGTWSNVWGACTTVRDFADLSFHSYFSIMEGMVE-WKGEESIDIKVYEIAGCILVAIV 123
           F DGTWS +  + T VRDF D+ FHSYFS+M+ + +     +  +I++ ++ G ++V+++
Sbjct: 134 FYDGTWSTMQRSFTVVRDFKDVCFHSYFSLMDELKQSCPDRKYYEIRLLQLPGALVVSVL 193

Query: 124 GILIDFPIITLIVLYKVPIMLVKGWHRLIQDLIGREGPFLETVCVPFAGLWILLWPAVVL 183
           GIL+D P+I+L+ + K P ML KGWHRL  DLIGREGPFLET+CVP AGL ILLWP  V 
Sbjct: 194 GILVDPPVISLVAICKSPYMLFKGWHRLFHDLIGREGPFLETMCVPIAGLAILLWPLAVT 253

Query: 184 IAAFAGITSSIGYGCYAAVVAYQESSLKRGLLYVVASISLFDEYTNDLLYLREGSCFPRP 243
            A    + SSI  G YA VV+YQESS   GL Y+VAS+S++DEY+ D+L L EGSCFPRP
Sbjct: 254 GAVIGSVISSIFLGAYAGVVSYQESSFYYGLCYIVASVSIYDEYSTDILDLPEGSCFPRP 313

Query: 244 KYREKMDTS---SSMLPVQEFRKHLEAIHTGEPLIRNMAEHKSVVKAVVILDGFYKACED 303
           KYR K +     S  +P     K+  ++  G   +  +      +K + +L+  +  C  
Sbjct: 314 KYRRKDEEPTPFSGPVPRLGSVKNASSMRGGSVRVPMID-----IKPLDLLNELFVECRR 373

Query: 304 IGKDLIRDEAIGLADLEAWRHAE-NKIVNIGIPSHAFLQCFLRSIKSGSTGFLMRDNI-E 363
            G+ L     I   D+E  R ++ ++++++G+P++  L   LRS+K+ S+G L+ D + E
Sbjct: 374 YGEVLATKGLINSKDIEEARSSKGSQVISVGLPAYGLLYEILRSVKANSSGLLLSDGVTE 433

Query: 364 ITSVNRPEGRIFDWLYEPMSIMKEQIRRLNLQESEELYLYKLCLYSGDMTRVESWHN-GG 423
           IT++NRP+   FDW   P  I+KEQ++  NL E EE YL +L L  GD  R++S +    
Sbjct: 434 ITTMNRPKDVFFDWFLNPFLILKEQMKATNLSEEEEEYLGRLVLLFGDPERLKSSNAISA 493

Query: 424 VPPPDEIRRAQLEGISRR 435
            PP  E +RA+L+  +RR
Sbjct: 494 SPPLTERKRAELDAFARR 503

BLAST of Lag0008221 vs. ExPASy TrEMBL
Match: A0A6J1DVH5 (uncharacterized membrane protein At3g27390-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111024758 PE=4 SV=1)

HSP 1 Score: 759.2 bits (1959), Expect = 9.6e-216
Identity = 363/434 (83.64%), Postives = 399/434 (91.94%), Query Frame = 0

Query: 1   MFRPFTKCLMILVLPVPIALWIVIGVIGSVVMGIGYAFVWPFMETFRAISREGVPVYMRL 60
           MF PFTK L+IL+LP+PIA+W VIGV+GSV+MGIGY FVWPFMETFRAISREGVP+YMRL
Sbjct: 71  MFGPFTKFLLILILPIPIAIWTVIGVVGSVIMGIGYGFVWPFMETFRAISREGVPIYMRL 130

Query: 61  IRCFTDGTWSNVWGACTTVRDFADLSFHSYFSIMEGMVEWKGEESIDIKVYEIAGCILVA 120
           IRCFTDGTWSNVWGACT VRDFAD SFHSYFSIM+ MVEWKGEE +DIKVYE+AGC+LVA
Sbjct: 131 IRCFTDGTWSNVWGACTIVRDFADFSFHSYFSIMDQMVEWKGEEPVDIKVYEVAGCVLVA 190

Query: 121 IVGILIDFPIITLIVLYKVPIMLVKGWHRLIQDLIGREGPFLETVCVPFAGLWILLWPAV 180
           IVGI++DFPII L+VLYK PIMLVKGWHRLIQDL+GREGPFLETVCVPFAGLWILLWP V
Sbjct: 191 IVGIVVDFPIIPLLVLYKAPIMLVKGWHRLIQDLVGREGPFLETVCVPFAGLWILLWPFV 250

Query: 181 VLIAAFAGITSSIGYGCYAAVVAYQESSLKRGLLYVVASISLFDEYTNDLLYLREGSCFP 240
           VL+A+FAGITS IG+GCYAAVVA+QE+SLKRGLLYV+ASISLFDEYTNDLLYLREGSCFP
Sbjct: 251 VLLASFAGITSIIGFGCYAAVVAFQENSLKRGLLYVIASISLFDEYTNDLLYLREGSCFP 310

Query: 241 RPKYREKMDTSSSMLPVQEFRKHLEAIHTGEPLIRNMAEHKSVVKAVVILDGFYKACEDI 300
           RPKYREKMD+SS ++P +   +HL+AIHT EPLI   AE KS VKAVVI D F+KAC DI
Sbjct: 311 RPKYREKMDSSSLLVPGKGLHEHLQAIHTREPLIMTTAEQKSAVKAVVIWDSFFKACVDI 370

Query: 301 GKDLIRDEAIGLADLEAWRHAENKIVNIGIPSHAFLQCFLRSIKSGSTGFLMRDNIEITS 360
           GK+LIRDEAIGLADLEAWRHA+NKIVN+GIPSH FLQCFLRSIK GS GFLMRD++EIT 
Sbjct: 371 GKELIRDEAIGLADLEAWRHAKNKIVNVGIPSHVFLQCFLRSIKRGSVGFLMRDDVEITH 430

Query: 361 VNRPEGRIFDWLYEPMSIMKEQIRRLNLQESEELYLYKLCLYSGDMTRVESWHNGGVPPP 420
           VNRPEGRIFDWLYEPMSIMKEQIRRLNLQE+E+LYLYK CLYSGD  R+ESW NGGVP P
Sbjct: 431 VNRPEGRIFDWLYEPMSIMKEQIRRLNLQENEQLYLYKFCLYSGDTKRIESWENGGVPSP 490

Query: 421 DEIRRAQLEGISRR 435
           DEIRRAQLEGISRR
Sbjct: 491 DEIRRAQLEGISRR 504

BLAST of Lag0008221 vs. ExPASy TrEMBL
Match: A0A6J1E0C4 (uncharacterized membrane protein At3g27390-like isoform X1 OS=Momordica charantia OX=3673 GN=LOC111024761 PE=4 SV=1)

HSP 1 Score: 724.2 bits (1868), Expect = 3.4e-205
Identity = 351/435 (80.69%), Postives = 386/435 (88.74%), Query Frame = 0

Query: 1   MFRPFTKCLMILVLPVPIALWIVIGVIGSVVMGIGYAFVWPFMETFRAISREGVPVYMRL 60
           MF PF KCL+IL+LP+PIA+W VIGV+GSV+MGIGY FVWP MET RAISREG+P++MRL
Sbjct: 71  MFGPFMKCLLILILPIPIAVWTVIGVVGSVIMGIGYGFVWPVMETLRAISREGIPIHMRL 130

Query: 61  IRCFTDGTWSNVWGACTTVRDFADLSFHSYFSIMEGMVEWKGEESIDIKVYEIAGCILVA 120
           IRCFTDGTWSNVWGACT VRDFAD SFHSYFSIM+GMVEW+GEE IDIKV+E+A CILVA
Sbjct: 131 IRCFTDGTWSNVWGACTIVRDFADFSFHSYFSIMDGMVEWQGEEQIDIKVHELASCILVA 190

Query: 121 IVGILIDFPIITLIVLYKVPIMLVKGWHRLIQDLIGREGPFLETVCVPFAGLWILLWPAV 180
           +VGI++DFPIITLIVLYK PIML KGWHRLIQDLIGREGPFLETVCVPFAGLWILLWP  
Sbjct: 191 LVGIMVDFPIITLIVLYKAPIMLFKGWHRLIQDLIGREGPFLETVCVPFAGLWILLWPVA 250

Query: 181 VLIAAFAGITSSIGYGCYAAVVAYQESSLKRGLLYVVASISLFDEYTNDLLYLREGSCFP 240
           V++A FAGI SSIG G Y  VV YQESSLKRGLLYV+ASISLFDEYTNDLLYLREGSCFP
Sbjct: 251 VIMATFAGIASSIGLGLYGVVVTYQESSLKRGLLYVIASISLFDEYTNDLLYLREGSCFP 310

Query: 241 RPKYREKMD-TSSSMLPVQEFRKHLEAIHTGEPLIRNMAEHKSVVKAVVILDGFYKACED 300
           RPKY EKMD +SSS++PV+   +HLE I T EPL+   AE KS VKAVVI D F+KACE+
Sbjct: 311 RPKYSEKMDYSSSSLVPVKGLHEHLERIRTSEPLM-TTAEQKSAVKAVVIWDSFFKACEE 370

Query: 301 IGKDLIRDEAIGLADLEAWRHAENKIVNIGIPSHAFLQCFLRSIKSGSTGFLMRDNIEIT 360
           IGK+LIRDEAIG+ADLEAWR A NKI+++GI SHAFLQCFLRSIKSGS GFLM D I IT
Sbjct: 371 IGKELIRDEAIGVADLEAWRQARNKIIHVGISSHAFLQCFLRSIKSGSAGFLMSDKIVIT 430

Query: 361 SVNRPEGRIFDWLYEPMSIMKEQIRRLNLQESEELYLYKLCLYSGDMTRVESWHNGGVPP 420
           SVNRPEGRIFDWLYEP+SIMKEQIR LNLQESEELYLYK CLYSGD  R+ESW NGG+PP
Sbjct: 431 SVNRPEGRIFDWLYEPLSIMKEQIRCLNLQESEELYLYKFCLYSGDTARIESWENGGIPP 490

Query: 421 PDEIRRAQLEGISRR 435
           PDEIRRAQLEGI RR
Sbjct: 491 PDEIRRAQLEGIGRR 504

BLAST of Lag0008221 vs. ExPASy TrEMBL
Match: A0A6J1DV88 (uncharacterized membrane protein At3g27390-like isoform X3 OS=Momordica charantia OX=3673 GN=LOC111024758 PE=4 SV=1)

HSP 1 Score: 716.8 bits (1849), Expect = 5.5e-203
Identity = 341/403 (84.62%), Postives = 371/403 (92.06%), Query Frame = 0

Query: 32  MGIGYAFVWPFMETFRAISREGVPVYMRLIRCFTDGTWSNVWGACTTVRDFADLSFHSYF 91
           MGIGY FVWPFMETFRAISREGVP+YMRLIRCFTDGTWSNVWGACT VRDFAD SFHSYF
Sbjct: 1   MGIGYGFVWPFMETFRAISREGVPIYMRLIRCFTDGTWSNVWGACTIVRDFADFSFHSYF 60

Query: 92  SIMEGMVEWKGEESIDIKVYEIAGCILVAIVGILIDFPIITLIVLYKVPIMLVKGWHRLI 151
           SIM+ MVEWKGEE +DIKVYE+AGC+LVAIVGI++DFPII L+VLYK PIMLVKGWHRLI
Sbjct: 61  SIMDQMVEWKGEEPVDIKVYEVAGCVLVAIVGIVVDFPIIPLLVLYKAPIMLVKGWHRLI 120

Query: 152 QDLIGREGPFLETVCVPFAGLWILLWPAVVLIAAFAGITSSIGYGCYAAVVAYQESSLKR 211
           QDL+GREGPFLETVCVPFAGLWILLWP VVL+A+FAGITS IG+GCYAAVVA+QE+SLKR
Sbjct: 121 QDLVGREGPFLETVCVPFAGLWILLWPFVVLLASFAGITSIIGFGCYAAVVAFQENSLKR 180

Query: 212 GLLYVVASISLFDEYTNDLLYLREGSCFPRPKYREKMDTSSSMLPVQEFRKHLEAIHTGE 271
           GLLYV+ASISLFDEYTNDLLYLREGSCFPRPKYREKMD+SS ++P +   +HL+AIHT E
Sbjct: 181 GLLYVIASISLFDEYTNDLLYLREGSCFPRPKYREKMDSSSLLVPGKGLHEHLQAIHTRE 240

Query: 272 PLIRNMAEHKSVVKAVVILDGFYKACEDIGKDLIRDEAIGLADLEAWRHAENKIVNIGIP 331
           PLI   AE KS VKAVVI D F+KAC DIGK+LIRDEAIGLADLEAWRHA+NKIVN+GIP
Sbjct: 241 PLIMTTAEQKSAVKAVVIWDSFFKACVDIGKELIRDEAIGLADLEAWRHAKNKIVNVGIP 300

Query: 332 SHAFLQCFLRSIKSGSTGFLMRDNIEITSVNRPEGRIFDWLYEPMSIMKEQIRRLNLQES 391
           SH FLQCFLRSIK GS GFLMRD++EIT VNRPEGRIFDWLYEPMSIMKEQIRRLNLQE+
Sbjct: 301 SHVFLQCFLRSIKRGSVGFLMRDDVEITHVNRPEGRIFDWLYEPMSIMKEQIRRLNLQEN 360

Query: 392 EELYLYKLCLYSGDMTRVESWHNGGVPPPDEIRRAQLEGISRR 435
           E+LYLYK CLYSGD  R+ESW NGGVP PDEIRRAQLEGISRR
Sbjct: 361 EQLYLYKFCLYSGDTKRIESWENGGVPSPDEIRRAQLEGISRR 403

BLAST of Lag0008221 vs. ExPASy TrEMBL
Match: A0A2N9J9T7 (Uncharacterized protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS61988 PE=4 SV=1)

HSP 1 Score: 687.2 bits (1772), Expect = 4.6e-194
Identity = 323/433 (74.60%), Postives = 380/433 (87.76%), Query Frame = 0

Query: 2   FRPFTKCLMILVLPVPIALWIVIGVIGSVVMGIGYAFVWPFMETFRAISREGVPVYMRLI 61
           F P+ KCL+IL++P+PIALW V+GV GS +MGIGY FVWP +ETFRAIS+EGVP++M+LI
Sbjct: 44  FGPYMKCLVILIVPIPIALWTVVGVAGSAIMGIGYGFVWPVLETFRAISKEGVPIHMKLI 103

Query: 62  RCFTDGTWSNVWGACTTVRDFADLSFHSYFSIMEGMVEWKGEESIDIKVYEIAGCILVAI 121
           RCFTDGTWSNVWGACT VRDFAD SFHSYFS+M+GM+E KGEE I++KV +I GCIL A 
Sbjct: 104 RCFTDGTWSNVWGACTIVRDFADFSFHSYFSVMDGMLESKGEEPIELKVTQIPGCILAAT 163

Query: 122 VGILIDFPIITLIVLYKVPIMLVKGWHRLIQDLIGREGPFLETVCVPFAGLWILLWPAVV 181
           +G+L+D PIITLIVLYK P++L KGWHRLIQDLIGREGPFLETVCVPFA +WILLWP VV
Sbjct: 164 LGVLVDVPIITLIVLYKTPVLLYKGWHRLIQDLIGREGPFLETVCVPFAAIWILLWPIVV 223

Query: 182 LIAAFAGITSSIGYGCYAAVVAYQESSLKRGLLYVVASISLFDEYTNDLLYLREGSCFPR 241
           L+A   GI SS+G+GCYAAVVAYQE+  KRGLLYV+AS+S+FDEYTNDLLY+REGSCFPR
Sbjct: 224 LLATLVGILSSLGFGCYAAVVAYQENMTKRGLLYVIASLSIFDEYTNDLLYMREGSCFPR 283

Query: 242 PKYREKMDTSSSMLPVQEFRKHLEAIHTGEPLIRNMAEHKSVVKAVVILDGFYKACEDIG 301
           P+YRE++ +SSS+LPV+   + +EAI T EPLIR     K+ V+AVVI DGF+KACEDIG
Sbjct: 284 PRYRERVTSSSSLLPVKGLHEQVEAIRTNEPLIRT-PNTKNTVQAVVIWDGFFKACEDIG 343

Query: 302 KDLIRDEAIGLADLEAWRHAENKIVNIGIPSHAFLQCFLRSIKSGSTGFLMRDNIEITSV 361
           K+L+RD AIGL DL+AWRH++NKI NIGIP++AFL CFLRSIKSGS GFLMRD+IEITSV
Sbjct: 344 KELLRDGAIGLQDLDAWRHSKNKIANIGIPAYAFLDCFLRSIKSGSIGFLMRDSIEITSV 403

Query: 362 NRPEGRIFDWLYEPMSIMKEQIRRLNLQESEELYLYKLCLYSGDMTRVESWHNGGVPPPD 421
           NRPEGR+FDWLYEPM I+KEQIR LNLQE+EELY YK CLYSGD  R+ESW NGG+PP D
Sbjct: 404 NRPEGRVFDWLYEPMCIIKEQIRSLNLQETEELYFYKHCLYSGDAARIESWKNGGIPPHD 463

Query: 422 EIRRAQLEGISRR 435
           EI+RAQLEGISRR
Sbjct: 464 EIKRAQLEGISRR 475

BLAST of Lag0008221 vs. ExPASy TrEMBL
Match: A0A6J1DVI0 (uncharacterized membrane protein At3g27390-like isoform X2 OS=Momordica charantia OX=3673 GN=LOC111024761 PE=4 SV=1)

HSP 1 Score: 680.2 bits (1754), Expect = 5.7e-192
Identity = 329/404 (81.44%), Postives = 358/404 (88.61%), Query Frame = 0

Query: 32  MGIGYAFVWPFMETFRAISREGVPVYMRLIRCFTDGTWSNVWGACTTVRDFADLSFHSYF 91
           MGIGY FVWP MET RAISREG+P++MRLIRCFTDGTWSNVWGACT VRDFAD SFHSYF
Sbjct: 1   MGIGYGFVWPVMETLRAISREGIPIHMRLIRCFTDGTWSNVWGACTIVRDFADFSFHSYF 60

Query: 92  SIMEGMVEWKGEESIDIKVYEIAGCILVAIVGILIDFPIITLIVLYKVPIMLVKGWHRLI 151
           SIM+GMVEW+GEE IDIKV+E+A CILVA+VGI++DFPIITLIVLYK PIML KGWHRLI
Sbjct: 61  SIMDGMVEWQGEEQIDIKVHELASCILVALVGIMVDFPIITLIVLYKAPIMLFKGWHRLI 120

Query: 152 QDLIGREGPFLETVCVPFAGLWILLWPAVVLIAAFAGITSSIGYGCYAAVVAYQESSLKR 211
           QDLIGREGPFLETVCVPFAGLWILLWP  V++A FAGI SSIG G Y  VV YQESSLKR
Sbjct: 121 QDLIGREGPFLETVCVPFAGLWILLWPVAVIMATFAGIASSIGLGLYGVVVTYQESSLKR 180

Query: 212 GLLYVVASISLFDEYTNDLLYLREGSCFPRPKYREKMD-TSSSMLPVQEFRKHLEAIHTG 271
           GLLYV+ASISLFDEYTNDLLYLREGSCFPRPKY EKMD +SSS++PV+   +HLE I T 
Sbjct: 181 GLLYVIASISLFDEYTNDLLYLREGSCFPRPKYSEKMDYSSSSLVPVKGLHEHLERIRTS 240

Query: 272 EPLIRNMAEHKSVVKAVVILDGFYKACEDIGKDLIRDEAIGLADLEAWRHAENKIVNIGI 331
           EPL+   AE KS VKAVVI D F+KACE+IGK+LIRDEAIG+ADLEAWR A NKI+++GI
Sbjct: 241 EPLM-TTAEQKSAVKAVVIWDSFFKACEEIGKELIRDEAIGVADLEAWRQARNKIIHVGI 300

Query: 332 PSHAFLQCFLRSIKSGSTGFLMRDNIEITSVNRPEGRIFDWLYEPMSIMKEQIRRLNLQE 391
            SHAFLQCFLRSIKSGS GFLM D I ITSVNRPEGRIFDWLYEP+SIMKEQIR LNLQE
Sbjct: 301 SSHAFLQCFLRSIKSGSAGFLMSDKIVITSVNRPEGRIFDWLYEPLSIMKEQIRCLNLQE 360

Query: 392 SEELYLYKLCLYSGDMTRVESWHNGGVPPPDEIRRAQLEGISRR 435
           SEELYLYK CLYSGD  R+ESW NGG+PPPDEIRRAQLEGI RR
Sbjct: 361 SEELYLYKFCLYSGDTARIESWENGGIPPPDEIRRAQLEGIGRR 403

BLAST of Lag0008221 vs. TAIR 10
Match: AT4G12680.1 (unknown protein; INVOLVED IN: vegetative to reproductive phase transition of meristem; LOCATED IN: endomembrane system; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G40640.1); Has 103 Blast hits to 103 proteins in 14 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 103; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 345.5 bits (885), Expect = 6.4e-95
Identity = 186/439 (42.37%), Postives = 267/439 (60.82%), Query Frame = 0

Query: 7   KCLMILVLPVPIALWIVIGVIGSVVMGIGYAFVWPFMETFRAISREGVPVYMRLIRCFTD 66
           K L +++ P+P+ LW V G++GS+  GI Y F  P M TF A+   G  V  +   CF D
Sbjct: 77  KTLALVLFPLPLLLWPVAGIVGSLFGGIAYGFFTPLMATFEAV---GESVTSKCYHCFVD 136

Query: 67  GTWSNVWGACTTVRDFADLSFHSYFSIMEGMVEWKGE--ESIDIKVYEIAGCILVAIVGI 126
           G++S + G+CT V DF D  FHSYFS M+ + E      E ++IK+  +  C+L +++G+
Sbjct: 137 GSFSTIKGSCTVVTDFTDFCFHSYFSYMDELREMVSADVEPLEIKLSRLPSCLLASLIGV 196

Query: 127 LIDFPIITLIVLYKVPIMLVKGWHRLIQDLIGREGPFLETVCVPFAGLWILLWPAVVLIA 186
           ++D  +IT + +YK P ML+KGW RL++DL+GREGPFLE+VCVPFAGL ILLWP  V  A
Sbjct: 197 MVDVLLITAVAVYKSPYMLLKGWKRLLEDLVGREGPFLESVCVPFAGLAILLWPLAVAGA 256

Query: 187 AFAGITSSIGYGCYAAVVAYQESSLKRGLLYVVASISLFDEYTNDLLYLREGSCFPRPKY 246
             A + SS   G Y+ V+ +QE S + GL Y++A++SLFDEY NDLLYLREG+  PRP Y
Sbjct: 257 VIASVLSSFFLGLYSGVIVHQEDSFRMGLNYIIAAVSLFDEYVNDLLYLREGTSLPRPCY 316

Query: 247 REKMDTSSSMLPVQEFR----KHLEAIHTGEPLI----RNMAEHKSVVKAVVILDGFYKA 306
           R K +T      + E +    K   +   G  L+    R + +  ++ K V + +  +K+
Sbjct: 317 RTKTETVHGKRILGESKNVDLKSKRSSSLGSKLVSEQSRTLKKAITLYKPVQVWEWLFKS 376

Query: 307 CEDIGKDLIRDEAIGLADL-EAWRHAENKIVNIGIPSHAFLQCFLRSIKSGSTGFLMRDN 366
           CE  G+ L+RD  I + D+ E      +K + I +P+   LQC L S KS S+G ++ D 
Sbjct: 377 CEVNGRILLRDGLIDVKDVEECLVKGNSKKLYIKLPAWTVLQCLLASAKSNSSGLVITDG 436

Query: 367 IEITSVNRPEGRIFDWLYEPMSIMKEQIRRLNLQESEELYLYKLCLYSGDMTRVESWHNG 426
           +E+T +N P  ++F WL  P+ IMKEQI+ L L E EE  L KL +   +  R E W N 
Sbjct: 437 VELTELNSPRDKVFVWLVGPLLIMKEQIKNLKLTEDEEFCLRKLVMVCKN-ERTEDWDNT 496

Query: 427 GVPPPDEIRRAQLEGISRR 435
           G P  D +R+AQL+ I RR
Sbjct: 497 GFPSSDTVRKAQLQAIIRR 511

BLAST of Lag0008221 vs. TAIR 10
Match: AT3G27390.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: plasma membrane; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G40640.1); Has 101 Blast hits to 99 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 101; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 327.4 bits (838), Expect = 1.8e-89
Identity = 184/438 (42.01%), Postives = 268/438 (61.19%), Query Frame = 0

Query: 4   PFTKCLMILVLPVPIALWIVIGVIGSVVMGIGYAFVWPFMETFRAISREGVPVYMRLIRC 63
           P  K  + L LP  I LW ++G++GSV+ G  Y F  P   TF A+  EG P   +   C
Sbjct: 74  PILKIFLCLCLPAAIILWPIVGILGSVLGGALYGFFSPIFATFDAVG-EGKP--YQFFHC 133

Query: 64  FTDGTWSNVWGACTTVRDFADLSFHSYFSIMEGMVE-WKGEESIDIKVYEIAGCILVAIV 123
           F DGTWS +  + T VRDF D+ FHSYFS+M+ + +     +  +I++ ++ G ++V+++
Sbjct: 134 FYDGTWSTMQRSFTVVRDFKDVCFHSYFSLMDELKQSCPDRKYYEIRLLQLPGALVVSVL 193

Query: 124 GILIDFPIITLIVLYKVPIMLVKGWHRLIQDLIGREGPFLETVCVPFAGLWILLWPAVVL 183
           GIL+D P+I+L+ + K P ML KGWHRL  DLIGREGPFLET+CVP AGL ILLWP  V 
Sbjct: 194 GILVDPPVISLVAICKSPYMLFKGWHRLFHDLIGREGPFLETMCVPIAGLAILLWPLAVT 253

Query: 184 IAAFAGITSSIGYGCYAAVVAYQESSLKRGLLYVVASISLFDEYTNDLLYLREGSCFPRP 243
            A    + SSI  G YA VV+YQESS   GL Y+VAS+S++DEY+ D+L L EGSCFPRP
Sbjct: 254 GAVIGSVISSIFLGAYAGVVSYQESSFYYGLCYIVASVSIYDEYSTDILDLPEGSCFPRP 313

Query: 244 KYREKMDTS---SSMLPVQEFRKHLEAIHTGEPLIRNMAEHKSVVKAVVILDGFYKACED 303
           KYR K +     S  +P     K+  ++  G   +  +      +K + +L+  +  C  
Sbjct: 314 KYRRKDEEPTPFSGPVPRLGSVKNASSMRGGSVRVPMID-----IKPLDLLNELFVECRR 373

Query: 304 IGKDLIRDEAIGLADLEAWRHAE-NKIVNIGIPSHAFLQCFLRSIKSGSTGFLMRDNI-E 363
            G+ L     I   D+E  R ++ ++++++G+P++  L   LRS+K+ S+G L+ D + E
Sbjct: 374 YGEVLATKGLINSKDIEEARSSKGSQVISVGLPAYGLLYEILRSVKANSSGLLLSDGVTE 433

Query: 364 ITSVNRPEGRIFDWLYEPMSIMKEQIRRLNLQESEELYLYKLCLYSGDMTRVESWHN-GG 423
           IT++NRP+   FDW   P  I+KEQ++  NL E EE YL +L L  GD  R++S +    
Sbjct: 434 ITTMNRPKDVFFDWFLNPFLILKEQMKATNLSEEEEEYLGRLVLLFGDPERLKSSNAISA 493

Query: 424 VPPPDEIRRAQLEGISRR 435
            PP  E +RA+L+  +RR
Sbjct: 494 SPPLTERKRAELDAFARR 503

BLAST of Lag0008221 vs. TAIR 10
Match: AT5G40640.1 (unknown protein; LOCATED IN: plasma membrane; EXPRESSED IN: 19 plant structures; EXPRESSED DURING: 7 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT3G27390.1); Has 104 Blast hits to 102 proteins in 14 species: Archae - 0; Bacteria - 0; Metazoa - 2; Fungi - 0; Plants - 101; Viruses - 0; Other Eukaryotes - 1 (source: NCBI BLink). )

HSP 1 Score: 325.9 bits (834), Expect = 5.2e-89
Identity = 184/438 (42.01%), Postives = 263/438 (60.05%), Query Frame = 0

Query: 4   PFTKCLMILVLPVPIALWIVIGVIGSVVMGIGYAFVWPFMETFRAISREGVPVYMRLIRC 63
           P  K  + L +P+ + LW+V+ ++GSV+ G  Y F+ P   TF A+   G         C
Sbjct: 74  PILKIFLCLCVPLGVILWLVVSILGSVLGGAIYGFLSPIFATFDAV---GEGKSNPFFHC 133

Query: 64  FTDGTWSNVWGACTTVRDFADLSFHSYFSIMEGM-VEWKGEESIDIKVYEIAGCILVAIV 123
           F DGTWS V G+ T V DF D+ FHSYFS M+ +          +I++ +I G ++VA++
Sbjct: 134 FYDGTWSTVQGSFTVVCDFKDVCFHSYFSFMDDLRTSTANRHYYEIRLLQIPGAVIVAVL 193

Query: 124 GILIDFPIITLIVLYKVPIMLVKGWHRLIQDLIGREGPFLETVCVPFAGLWILLWPAVVL 183
           GIL+DFP+I+L+ L K P ML KGWHRL  DLIGREGPFLET+CVP AGL ILLWP  V+
Sbjct: 194 GILVDFPVISLLALCKSPYMLFKGWHRLFHDLIGREGPFLETMCVPIAGLVILLWPLAVV 253

Query: 184 IAAFAGITSSIGYGCYAAVVAYQESSLKRGLLYVVASISLFDEYTNDLLYLREGSCFPRP 243
            A    + SS+  G Y  VV+YQESS   GL YVVAS+S++DEY+ND+L + EGSCFPRP
Sbjct: 254 GAVLGSVVSSVFLGAYGGVVSYQESSFFFGLCYVVASVSIYDEYSNDVLDMPEGSCFPRP 313

Query: 244 KYREKMDTSSSMLPVQEFRKHLEAIHTGEPLIRNMAEHKSV---VKAVVILDGFYKACED 303
            YR   + +S+      F   L   ++ +        +K     +K + +L+  +  C  
Sbjct: 314 IYRRNEEGAST-----AFSGGLSRPNSFKTTPSRGGSNKGPMIDLKPLDLLEALFVECRR 373

Query: 304 IGKDLIRDEAIGLADLEAWRHAE-NKIVNIGIPSHAFLQCFLRSIKSGSTGFLMRDNI-E 363
            G+ ++    I   D+E  + ++ +++++ G+P+++ L   LRSIKS STG L+ D + E
Sbjct: 374 HGEIMVTKGIINSKDIEEAKSSKGSQVISFGLPAYSLLHELLRSIKSNSTGLLLGDGVTE 433

Query: 364 ITSVNRPEGRIFDWLYEPMSIMKEQIRRLNLQESEELYLYKLCLYSGDMTRVE-SWHNGG 423
           IT+ NRP+   FDW   P  I+K+QI   NL E EE YL KL L  GD  R++ S     
Sbjct: 434 ITTRNRPKDAFFDWFLNPFLILKDQIEAANLSEEEEEYLGKLVLLFGDSERLKSSIVESE 493

Query: 424 VPPPDEIRRAQLEGISRR 435
            PP  E+R+A+L+  +RR
Sbjct: 494 SPPLTELRKAELDAFARR 503

BLAST of Lag0008221 vs. TAIR 10
Match: AT4G37030.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT4G12680.1); Has 101 Blast hits to 99 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 101; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 314.3 bits (804), Expect = 1.6e-85
Identity = 172/445 (38.65%), Postives = 261/445 (58.65%), Query Frame = 0

Query: 7   KCLMILVLPVPIALWIVIGVIGSVVMGIGYAFVWPFMETFRAISREGVPVYMRLIRCFTD 66
           K  +++ LP    +W+ + +  SV++G+GY F  P++  F A  ++      +   C  D
Sbjct: 77  KVAILVALPALFGIWLGLSLAISVLVGVGYGFFTPWISAFEAFRQD--TESNKFFHCLVD 136

Query: 67  GTWSNVWGACTTVRDFADLSFHSYFSIMEGMVEWK-GEESIDIKVYEIAGCILVAIVGIL 126
           GTW  + G+C  V DFAD  +HSY   ++ + E    +E   +++  + GCI+V I+G++
Sbjct: 137 GTWGTIKGSCIVVTDFADFCYHSYPLYLKELRESPVSDELQTLRLIHVPGCIIVGILGLV 196

Query: 127 IDFPIITLIVLYKVPIMLVKGWHRLIQDLIGREGPFLETVCVPFAGLWILLWPAVVLIAA 186
           ID P+ T I + K P +L+KGW+RL QD I REGPFLE  C+P AGL +LLWP VV+   
Sbjct: 197 IDIPLFTAIAVIKSPYLLLKGWYRLAQDAINREGPFLEIACIPVAGLTVLLWPIVVIGFI 256

Query: 187 FAGITSSIGYGCYAAVVAYQESSLKRGLLYVVASISLFDEYTNDLLYLREGSCFPRPKYR 246
              I SSI  G Y AVV +QE S +RG+ YV+A +  FDEYTND LYLREG+ FP+P+YR
Sbjct: 257 LVTIFSSIFVGLYGAVVVFQERSFRRGVSYVIAVVGEFDEYTNDWLYLREGTIFPKPRYR 316

Query: 247 EKMDTSSSMLPVQEFRKHLEAIHTG----------EPLIRNMAEHKSV--VKAVVILDGF 306
               + SS + V      +  +++             L+ +++  +++  V+ V I +  
Sbjct: 317 MGRGSFSSEVSVIVHPSDVTRVNSSGSVDAPAMLVPSLVHSVSVREAIQEVRMVQIWEHM 376

Query: 307 YKACEDIGKDLIRDEAIGLADLE---AWRHA-ENKIVNIGIPSHAFLQCFLRSIKSGSTG 366
               E  GK+L+  E +   DL      RH  E+ I+N+G+PS+A L   L SIK+G  G
Sbjct: 377 MGWFEMQGKELLDAEVLTPTDLYESLKGRHGNESSIINVGLPSYALLHTLLSSIKAGVHG 436

Query: 367 FLMRDNIEITSVNRPEGRIFDWLYEPMSIMKEQIRRLNLQESEELYLYKLCLYSGDMTRV 426
            L+ D  E+T +NRP+ +  DW++ P+ ++K+QIR L L ESE  YL K+ L+     R+
Sbjct: 437 VLLLDGSEVTHLNRPQDKFLDWVFNPIMVLKDQIRALKLGESEVKYLEKVVLFGNHEQRM 496

Query: 427 ESWHNGGVPPPDEIRRAQLEGISRR 435
           E+W N   PP + +R AQ++GISRR
Sbjct: 497 EAWDNHSNPPQENLRTAQIQGISRR 519