Lag0008219 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0008219 vs. NCBI nr
Match: KAF4391976.1 (hypothetical protein F8388_004305 [Cannabis sativa])

HSP 1 Score: 102.4 bits (254), Expect = 6.2e-18
Identity = 68/274 (24.82%), Postives = 130/274 (47.45%), Query Frame = 0

Query: 10  SQSDNSKQA-LFWKSLWKSNVLPRSKVCAWKIVNDIIPSKANILKKGVDLNPLCGICGKK 69
           ++S N  Q   +WK  W  N+ PR K+  WK+  + +P+K N++ +G+ +NP+C  CG+ 
Sbjct: 112 TRSSNMDQTHAWWKMWWGLNLPPRMKLFGWKMCRNWLPAKTNLVHRGMKINPICTNCGRF 171

Query: 70  LESTTHPIWECKIPREGWTRCLPSSLNLFSLCRDNWTTKDSWSRMMINLSKEDLDKGITI 129
            E+ +H +W C+  ++ W   L  S N+    R N +  D        L+KE+ +  + +
Sbjct: 172 DETLSHALWNCEKVKKVWK--LMPSYNIIKDSRGN-SMMDLLVEFKHKLAKEEFEDVVKV 231

Query: 130 MWKIWNFHNHNGDHNQI----RAIEYLVHDI------QTSITECEASYLKTQ-------- 189
           +W IW   N    +  I    R ++++ +        Q ++T+   +  K Q        
Sbjct: 232 LWAIWENRNRQWTNQHIMNGARLLDWVFNSYPREIHGQENLTKMHPAAEKNQWQTPPVGT 291

Query: 190 ------ASKRPGNQG-GLRWIVRDSNGSLVYFGMQKITRKSPIKILEAKEILAGLKHIVG 249
                 A+ +P  +G G+ ++ +D  G+++  GM+       + I EA+ + A L     
Sbjct: 292 YCVHCDAALKPNQEGVGIGYVWQDWKGNILSAGMKYQPIFCNVNIAEAQAVAAALNDKPK 351

Query: 250 TCNQRSIPLEVESDALEVVNVIVEVAEDLSDLKP 258
           + +    P E+ +D+  +V+ IV     L D+KP
Sbjct: 352 SVHH---PFEIRTDSKLLVDAIVHNGNSLDDMKP 379

BLAST of Lag0008219 vs. NCBI nr
Match: KAF4384439.1 (hypothetical protein G4B88_028513 [Cannabis sativa] >KAF4392273.1 hypothetical protein F8388_012729 [Cannabis sativa])

HSP 1 Score: 100.9 bits (250), Expect = 1.8e-17
Identity = 68/274 (24.82%), Postives = 125/274 (45.62%), Query Frame = 0

Query: 10  SQSDNSKQA-LFWKSLWKSNVLPRSKVCAWKIVNDIIPSKANILKKGVDLNPLCGICGKK 69
           ++S N  Q   +WK  W  N+ PR K+  WK+  + +P+K N++ +G+ +N +C  CG+ 
Sbjct: 138 TRSSNMDQTHAWWKMWWGLNLPPRMKLFGWKMCRNWLPAKTNLVHRGMKINSICTNCGRF 197

Query: 70  LESTTHPIWECKIPREGWTRCLPSSLNLFSLCRDNWTTKDSWSRMMINLSKEDLDKGITI 129
            E+ +H +W C+  ++ W   L  S N+    R N +  D        L KE+ +  + +
Sbjct: 198 DETLSHALWNCEKVKKVWK--LMPSYNIIKDSRGN-SMMDLLVEFKHKLVKEEFEDVVKV 257

Query: 130 MWKIWNFHNHNGDH----NQIRAIEYLVHDIQTSI-------------------TECEAS 189
           +W IW   N    +    N  R +E++ +     I                   T    +
Sbjct: 258 LWAIWENRNRQWTNQHTMNGARLLEWVFNSYPREIHGQENMAKIHPAAETDQWQTPSVGT 317

Query: 190 Y-LKTQASKRPGNQG-GLRWIVRDSNGSLVYFGMQKITRKSPIKILEAKEILAGLKHIVG 249
           Y +   A+ +P  +G GL ++ +D  G+++  GM+       +K+ EAK + A L     
Sbjct: 318 YCVHCDAALKPNQEGVGLGYVWQDWKGNIISAGMKYQPIFCNVKVAEAKAVAAALNDKPK 377

Query: 250 TCNQRSIPLEVESDALEVVNVIVEVAEDLSDLKP 258
             +    P E+ +D+ +++  IV     L D+KP
Sbjct: 378 NIHH---PFEIRTDSKQLMEAIVHNGNSLDDMKP 405

BLAST of Lag0008219 vs. NCBI nr
Match: XP_021847414.1 (uncharacterized protein LOC110787151 [Spinacia oleracea])

HSP 1 Score: 97.8 bits (242), Expect = 1.5e-16
Identity = 67/275 (24.36%), Postives = 112/275 (40.73%), Query Frame = 0

Query: 14   NSKQALFWKSLWKSNVLPRSKVCAWKIVNDIIPSKANILKKGVDLNPLCGICGKKLESTT 73
            +S  +  WK +WK NVLPR KV AW+  ++ +P++  + K+    + +CG+C  + ES  
Sbjct: 857  SSNPSSLWKQIWKLNVLPRVKVFAWRACHEALPTRKGLSKRVAGYDAVCGVCHCEDESVI 916

Query: 74   HPIWECKIPREGWTRCLPSSLNLFSLCRDNWTTKDSWSRMMINLSKEDLDKGITIMWKIW 133
            H + ECK+ R  W       +  +  C    +  D W R    L  E +++ IT+ W +W
Sbjct: 917  HAVRECKLARAIWCESRFEHMLSWQCC----SLVDWWDRGFKELDSEGMEEFITLCWAVW 976

Query: 134  NFHNHNGDHNQIRAIEYLVHD----IQTSITECEASYLKTQASKRPGNQGGLRW------ 193
               N          +E L  D    ++ + T C     +     R  + GG  W      
Sbjct: 977  GARNK-------AVMEGLTSDPTSTVKYATTVCNELRQEGVKGSRGSSGGGAEWAVEWSK 1036

Query: 194  ------------------------IVRDSNGSLVYFGMQKITRKSPIKILEAKEILAGLK 253
                                    ++RD  G +V   +++   K    + EAK +L G++
Sbjct: 1037 PEEGWYKVNVDAGLLGEVGSGMGAVIRDDEGGVVSCAVRQGCEKWATNVAEAKAVLFGME 1096

Query: 254  HIVGTCNQRSIPLEVESDALEVVNVIVEVAEDLSD 255
                   QR +   VESD L V+  +   A   S+
Sbjct: 1097 LCAELGMQRVV---VESDCLGVIQALRREASGCSE 1117

BLAST of Lag0008219 vs. NCBI nr
Match: KAF4399153.1 (hypothetical protein G4B88_023747 [Cannabis sativa])

HSP 1 Score: 93.6 bits (231), Expect = 2.9e-15
Identity = 82/306 (26.80%), Postives = 139/306 (45.42%), Query Frame = 0

Query: 2   KINAH--HSSSQSDNSKQALFWKSLWKSNVLPRSKVCAWKIVNDIIPSKANILKKGVDLN 61
           +IN H   SS+  D  K   +WK LW  ++ PR K+  W++ ++ +P+K N+  +G+D+N
Sbjct: 82  EINLHPTRSSNMEDIHK---WWKMLWSMSLPPRMKLFGWRVCHNWLPAKINLAHRGMDVN 141

Query: 62  PLCGICGKKLESTTHPIWECKIPREGWTRCLPSSLNLFSLCR--DNWTTKDSWSRMMINL 121
             C +CG + E+ TH +W C   +  W + +P     +  C    N +  D    +  +L
Sbjct: 142 LSCDLCGHQAETLTHALWGCAKVKTIW-KLVP----WYHKCAHFKNGSMFDIMVTLKDHL 201

Query: 122 SKEDLDKGITIMWKIWNFHNHNGDH----NQIRAIEYL---VHDIQTSITECEASYLKTQ 181
            K + ++ I IMW IW   N   +     N I+ ++++     D + S  +     +K Q
Sbjct: 202 HKSEFEEAIKIMWAIWENRNRYWNKLPVMNGIQLLDWIFTAYPDSRNSKEQTMNIDMKHQ 261

Query: 182 ASK---RP---------------GNQG-GLRWIVRDSNGSLVYFGMQKITRKSPIKILEA 241
             K   RP               G  G G  +I RD  G+L+  GM        +++ EA
Sbjct: 262 QPKKWIRPPTRHISVNCDAAMTNGTAGVGTGFIWRDWEGNLLLAGMVYHQSCCSVEMAEA 321

Query: 242 KEILAGLKHIVGTCNQRSIPLEVESDALEVV-------NVIVEVAEDLSDLKPNFYTSCI 271
             IL  LKH   T N   + +E++SD  ++V       N +  V+  L  ++    +S  
Sbjct: 322 WAILEALKHQPHTAN---MAIEIQSDCKKIVDEIQHRDNKLSAVSTILHQIQDRMDSSNC 376

BLAST of Lag0008219 vs. NCBI nr
Match: KAF4366980.1 (hypothetical protein F8388_022768 [Cannabis sativa])

HSP 1 Score: 93.6 bits (231), Expect = 2.9e-15
Identity = 61/262 (23.28%), Postives = 109/262 (41.60%), Query Frame = 0

Query: 20  FWKSLWKSNVLPRSKVCAWKIVNDIIPSKANILKKGVDLNPLCGICGKKLESTTHPIWEC 79
           +WK  WK  + PR K+  W++ N+ +P+K N+  +G+++NP+C +CG  +E+  H +W C
Sbjct: 435 WWKKFWKLQLPPRMKMFGWRVCNNWLPAKTNLCHRGMNINPVCDLCGYHVETLPHALWSC 494

Query: 80  KIPREGWTRCLPSSLNLFSLCRDNWTTKDSWSRMMINLSKEDLDKGITIMWKIWNFHNHN 139
              ++ W +  P       L     +  D  + +   LS+ + +  + IMW IW   N  
Sbjct: 495 DKVKQVW-KLTPWYKKCGKL--GEGSMFDILTSLREKLSRNEFEDALKIMWAIWENRNRK 554

Query: 140 GDHNQIRAIEYLVHDIQTSITECEASYLKTQASKRPGNQGGLRW---------------- 199
            +   +     L+  + T+     ++ ++ +  K       + W                
Sbjct: 555 WNKLHVMNGIQLLDWVFTTYPPASSNPMEEETKKVASPSNPMHWQAPHVGKICVNCDAAM 614

Query: 200 -----------IVRDSNGSLVYFGMQKITRKSPIKILEAKEILAGLKHIVGTCNQRSIPL 255
                      I RD NG+++  GM  I     I + EA EI   LK         + P+
Sbjct: 615 IPNQIGVGVGFIWRDWNGNVLNVGMIYIHSSCSINVAEAWEIHEALKK---HPRSETRPV 674

BLAST of Lag0008219 vs. ExPASy TrEMBL
Match: A0A7J6H9M1 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_004305 PE=4 SV=1)

HSP 1 Score: 102.4 bits (254), Expect = 3.0e-18
Identity = 68/274 (24.82%), Postives = 130/274 (47.45%), Query Frame = 0

Query: 10  SQSDNSKQA-LFWKSLWKSNVLPRSKVCAWKIVNDIIPSKANILKKGVDLNPLCGICGKK 69
           ++S N  Q   +WK  W  N+ PR K+  WK+  + +P+K N++ +G+ +NP+C  CG+ 
Sbjct: 112 TRSSNMDQTHAWWKMWWGLNLPPRMKLFGWKMCRNWLPAKTNLVHRGMKINPICTNCGRF 171

Query: 70  LESTTHPIWECKIPREGWTRCLPSSLNLFSLCRDNWTTKDSWSRMMINLSKEDLDKGITI 129
            E+ +H +W C+  ++ W   L  S N+    R N +  D        L+KE+ +  + +
Sbjct: 172 DETLSHALWNCEKVKKVWK--LMPSYNIIKDSRGN-SMMDLLVEFKHKLAKEEFEDVVKV 231

Query: 130 MWKIWNFHNHNGDHNQI----RAIEYLVHDI------QTSITECEASYLKTQ-------- 189
           +W IW   N    +  I    R ++++ +        Q ++T+   +  K Q        
Sbjct: 232 LWAIWENRNRQWTNQHIMNGARLLDWVFNSYPREIHGQENLTKMHPAAEKNQWQTPPVGT 291

Query: 190 ------ASKRPGNQG-GLRWIVRDSNGSLVYFGMQKITRKSPIKILEAKEILAGLKHIVG 249
                 A+ +P  +G G+ ++ +D  G+++  GM+       + I EA+ + A L     
Sbjct: 292 YCVHCDAALKPNQEGVGIGYVWQDWKGNILSAGMKYQPIFCNVNIAEAQAVAAALNDKPK 351

Query: 250 TCNQRSIPLEVESDALEVVNVIVEVAEDLSDLKP 258
           + +    P E+ +D+  +V+ IV     L D+KP
Sbjct: 352 SVHH---PFEIRTDSKLLVDAIVHNGNSLDDMKP 379

BLAST of Lag0008219 vs. ExPASy TrEMBL
Match: A0A7J6HAE9 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_012729 PE=4 SV=1)

HSP 1 Score: 100.9 bits (250), Expect = 8.8e-18
Identity = 68/274 (24.82%), Postives = 125/274 (45.62%), Query Frame = 0

Query: 10  SQSDNSKQA-LFWKSLWKSNVLPRSKVCAWKIVNDIIPSKANILKKGVDLNPLCGICGKK 69
           ++S N  Q   +WK  W  N+ PR K+  WK+  + +P+K N++ +G+ +N +C  CG+ 
Sbjct: 138 TRSSNMDQTHAWWKMWWGLNLPPRMKLFGWKMCRNWLPAKTNLVHRGMKINSICTNCGRF 197

Query: 70  LESTTHPIWECKIPREGWTRCLPSSLNLFSLCRDNWTTKDSWSRMMINLSKEDLDKGITI 129
            E+ +H +W C+  ++ W   L  S N+    R N +  D        L KE+ +  + +
Sbjct: 198 DETLSHALWNCEKVKKVWK--LMPSYNIIKDSRGN-SMMDLLVEFKHKLVKEEFEDVVKV 257

Query: 130 MWKIWNFHNHNGDH----NQIRAIEYLVHDIQTSI-------------------TECEAS 189
           +W IW   N    +    N  R +E++ +     I                   T    +
Sbjct: 258 LWAIWENRNRQWTNQHTMNGARLLEWVFNSYPREIHGQENMAKIHPAAETDQWQTPSVGT 317

Query: 190 Y-LKTQASKRPGNQG-GLRWIVRDSNGSLVYFGMQKITRKSPIKILEAKEILAGLKHIVG 249
           Y +   A+ +P  +G GL ++ +D  G+++  GM+       +K+ EAK + A L     
Sbjct: 318 YCVHCDAALKPNQEGVGLGYVWQDWKGNIISAGMKYQPIFCNVKVAEAKAVAAALNDKPK 377

Query: 250 TCNQRSIPLEVESDALEVVNVIVEVAEDLSDLKP 258
             +    P E+ +D+ +++  IV     L D+KP
Sbjct: 378 NIHH---PFEIRTDSKQLMEAIVHNGNSLDDMKP 405

BLAST of Lag0008219 vs. ExPASy TrEMBL
Match: A0A7J6FB29 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=F8388_022768 PE=4 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 1.4e-15
Identity = 61/262 (23.28%), Postives = 109/262 (41.60%), Query Frame = 0

Query: 20  FWKSLWKSNVLPRSKVCAWKIVNDIIPSKANILKKGVDLNPLCGICGKKLESTTHPIWEC 79
           +WK  WK  + PR K+  W++ N+ +P+K N+  +G+++NP+C +CG  +E+  H +W C
Sbjct: 435 WWKKFWKLQLPPRMKMFGWRVCNNWLPAKTNLCHRGMNINPVCDLCGYHVETLPHALWSC 494

Query: 80  KIPREGWTRCLPSSLNLFSLCRDNWTTKDSWSRMMINLSKEDLDKGITIMWKIWNFHNHN 139
              ++ W +  P       L     +  D  + +   LS+ + +  + IMW IW   N  
Sbjct: 495 DKVKQVW-KLTPWYKKCGKL--GEGSMFDILTSLREKLSRNEFEDALKIMWAIWENRNRK 554

Query: 140 GDHNQIRAIEYLVHDIQTSITECEASYLKTQASKRPGNQGGLRW---------------- 199
            +   +     L+  + T+     ++ ++ +  K       + W                
Sbjct: 555 WNKLHVMNGIQLLDWVFTTYPPASSNPMEEETKKVASPSNPMHWQAPHVGKICVNCDAAM 614

Query: 200 -----------IVRDSNGSLVYFGMQKITRKSPIKILEAKEILAGLKHIVGTCNQRSIPL 255
                      I RD NG+++  GM  I     I + EA EI   LK         + P+
Sbjct: 615 IPNQIGVGVGFIWRDWNGNVLNVGMIYIHSSCSINVAEAWEIHEALKK---HPRSETRPV 674

BLAST of Lag0008219 vs. ExPASy TrEMBL
Match: A0A7J6HVA4 (Uncharacterized protein OS=Cannabis sativa OX=3483 GN=G4B88_023747 PE=4 SV=1)

HSP 1 Score: 93.6 bits (231), Expect = 1.4e-15
Identity = 82/306 (26.80%), Postives = 139/306 (45.42%), Query Frame = 0

Query: 2   KINAH--HSSSQSDNSKQALFWKSLWKSNVLPRSKVCAWKIVNDIIPSKANILKKGVDLN 61
           +IN H   SS+  D  K   +WK LW  ++ PR K+  W++ ++ +P+K N+  +G+D+N
Sbjct: 82  EINLHPTRSSNMEDIHK---WWKMLWSMSLPPRMKLFGWRVCHNWLPAKINLAHRGMDVN 141

Query: 62  PLCGICGKKLESTTHPIWECKIPREGWTRCLPSSLNLFSLCR--DNWTTKDSWSRMMINL 121
             C +CG + E+ TH +W C   +  W + +P     +  C    N +  D    +  +L
Sbjct: 142 LSCDLCGHQAETLTHALWGCAKVKTIW-KLVP----WYHKCAHFKNGSMFDIMVTLKDHL 201

Query: 122 SKEDLDKGITIMWKIWNFHNHNGDH----NQIRAIEYL---VHDIQTSITECEASYLKTQ 181
            K + ++ I IMW IW   N   +     N I+ ++++     D + S  +     +K Q
Sbjct: 202 HKSEFEEAIKIMWAIWENRNRYWNKLPVMNGIQLLDWIFTAYPDSRNSKEQTMNIDMKHQ 261

Query: 182 ASK---RP---------------GNQG-GLRWIVRDSNGSLVYFGMQKITRKSPIKILEA 241
             K   RP               G  G G  +I RD  G+L+  GM        +++ EA
Sbjct: 262 QPKKWIRPPTRHISVNCDAAMTNGTAGVGTGFIWRDWEGNLLLAGMVYHQSCCSVEMAEA 321

Query: 242 KEILAGLKHIVGTCNQRSIPLEVESDALEVV-------NVIVEVAEDLSDLKPNFYTSCI 271
             IL  LKH   T N   + +E++SD  ++V       N +  V+  L  ++    +S  
Sbjct: 322 WAILEALKHQPHTAN---MAIEIQSDCKKIVDEIQHRDNKLSAVSTILHQIQDRMDSSNC 376

BLAST of Lag0008219 vs. ExPASy TrEMBL
Match: A0A6J1DL64 (uncharacterized protein LOC111022134 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111022134 PE=4 SV=1)

HSP 1 Score: 92.8 bits (229), Expect = 2.4e-15
Identity = 61/213 (28.64%), Postives = 98/213 (46.01%), Query Frame = 0

Query: 67  KKLESTTHPIWECKIPREGWTRCLPSSLNLFSLCRDNWTTKDSWSRMMINLSKEDLDKGI 126
           KK E+T H +WECK+ ++ W  C P   N F + R NWTTK+ W  +M    +E+  + +
Sbjct: 132 KKEETTQHILWECKVIKDIWINCTPIEANFFYIDRTNWTTKEYWEWLMDKAGEEERRRSM 191

Query: 127 TIMWKIWNFHNHN---GDHNQIRAIE-----YLV----------------HDIQTSITEC 186
            I  +IW   N +   G H++ R I+     Y++                H I+      
Sbjct: 192 IIACQIWEMRNKSIFKGVHSETRDIQLAIDRYIINSAGQDTNLKRKSKDFHPIRRIGDNT 251

Query: 187 EASY---------LKTQASKRPG-NQGGLRWIVRDSNGSLVYFGMQKITRKSPIKILEAK 246
            A +         L T A+ R   N  G+ WI+RD  G ++  G + I  +  I  LE  
Sbjct: 252 RARWKPPTSNSWKLNTDAAWRADTNTDGIGWILRDEKGEVIKTGCRIIRAERNITYLEVM 311

BLAST of Lag0008219 vs. TAIR 10
Match: AT3G26855.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 45.8 bits (107), Expect = 6.5e-05
Identity = 16/56 (28.57%), Postives = 31/56 (55.36%), Query Frame = 0

Query: 24 LWKSNVLPRSKVCAWKIVNDIIPSKANILKKGVDLNPLCGICGKKLESTTHPIWEC 80
          +W   + P+ K+  WK +N+ +P  A +L + + + P C  C +  E+ TH ++ C
Sbjct: 9  IWSLKISPKIKLLIWKALNNALPVGAQLLSRNISIEPFCTRC-RDFETITHILFNC 63