Lag0008018 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0008018
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	4-alpha-glucanotransferase
Location	chr9: 10078198 .. 10081023 (+)
RNA-Seq Expression	Lag0008018
Synteny	Lag0008018

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGCAAGAAGCCTCGATGGAATATCATGAAACTGAAAGGCCACATGGAGTACTTGGTAAGACCCAATTTTATTTATGCACTGTTTGATTCAAAACAAGGGAGACTTTTGAGAGCTCCCACTCTCCCAAGACACTCAAACCTGGGCCCTACATTTCAGACCAAAAATATACCTCGCCTCACTCCCAAAACATTCCTCTAAACCCAGAAAACGTCCCCCTAACTAACAATACTCCTTGGGAACCACAAGATCACAGAAACACATCTGCCACGTATTCCCTATGAGGCCATCCTCTACCAAATTTCTCACGTGTATTTTATCAGAATTTTGTGGTAATCAAGAATGGGAATGGAAGGACTTCATTTTTGGAATGGTAAATGGGTTTGAGACCAGCCACTATCATTTAGGTTCTCTGCAGATTTGATGCAATCATGGGATAGGAAAATGGTACTGTTAGCCAAGTTTGGAAGTATTACTTCGTGGAGAATTACTGCAAGGGAAAATTTACCATGAGAAGATGGAAAAGTTAAAGGAGCTTTTACAAGAGATTAATGGGGTACATATTGGAGGAAGCGGGAAGGGACAGAAAATTCTGGAAATTTTAAGTGTTTTTTCAGTGAGGTCTTTGATCGATCGTGGTGACAGATTTAAGAGTATGGCTAAAATTCTGTGCATTAAGGATATAAAAGCAATCATTTTCAGAATCTGGTGTGAAAGAATGCAGAGATTTTCTGAAAGATAGTGCCTAATTTGGGAAAAAGGTGTTGATAGATCAATTAGATACTACGCATCTTCTTGCTGAGCACTTTCGAGTCTGTTTTGTAACTATACTTCCAATGTAATTAATATTAATTGTGGTTGCCTTTTCTGGCAACATTTTTCTTTTTTTTTTGGGGGGGGGGGGGGGGGTTGGTAATTTTTTGCTAATTCTATGGAAATAGTAAAGAAATAAAGCCCATAGAAGAGTAAAATAAGGTTTGACATGTCTCGGTTGTTACAGGTACTGAAGTATCTTTCAATTAATGAGAAAGATGATATCCCCTGGGCTCTCATCCGGGCAGGATTATCTTCTGTGGCACAAACTGCCATAATACCTTTGCAAGATGTTCTTGGGTTAGGAACTTCTGCAAGGATGAACATTCCAGCAACACAGGTAAGTCATACCTTCTCAGATCTCCAATCAGTCTGGTTAACTGTCATAAATCTCAACATTAAGCATTTGAGTTGAATGCCATTCCAACATTTCATTGCTTGCATGACTAAAATCTGCACAAAAAATGATATGTTAAAAAATTTGTGTGATTTACAAATAATTTCAGTAGCTCATGAGTGCACACTGGTGTTTTCTTTAGTACCAGAAGATAACTATATTTTTTCCAACCACCCACAAAATCCCCCACCTTTTGATTCTTTAATTTTATACCATCTCAATGTGTAGTGTAGTGCATGAACAAAAACCATCAACCATTCATTCAGTAGACACCCCTTTATTTTGAAGAAAAATGTGAATAATTTTAGATTCTCTAAGAATGCAATATGATATCTATGGATTTGATTGTTATGATGGTTCAGTGTGTAAGATATATTTGTACTTGTCACAGTTTGGAAACTGGAAATGGAGAATACCTAGTTCCATAAGCTTTGAGAGTTTGGAGACAGAAGCTACTAAATTGAAGGATATGGTTTCATTGTATGGGCGGCTGTAAGTGCAAATGCTGGGCTTAATCACAAATTGTTCTTTTAGACCTTTAGTCTCATAAAGTAAATAGTTGTCTGAGCTTTAAGATTGTCTAATTTAATAAGGTCCCCCACCAAAAGTCTCGATGTGGTTATGTGCGATCCACAGGAGAAATTACAAGTTTGTGAAGATTCACAAACATTAGTCGAAGTAATAAATACAGACTCAACATTTCTTTCGCTATATACCATCTTTATCAACGAGTTCTAAATTATCATTATTAGCGTGTGTATCAATATGGTTGATTCTTCATCAATTTTTTCTTTCATGCCATCTTGAAAAAATGTTTTGTTCTTTCATATTATGTGGAAATATCGAGATGAAAATTAGTTTAAATACTATTTTGGTGTAGGTAATTTCAACCTTAGTTTATTACCCGTATAGTACAGTTAAAATATTCCTTTTGATCCTTATACTTTCAACTTTTATTTACTTTACTTCCTCTACTTTCAAAAAGTATGTTTTAGTTTCTATACTTTTAAAAAGTGACTATTTTAGTTGTTTAAAAATTCTCACTAGTTCTATACACAATGGAAACACTTTAATATATAGTTTTGGCCATATTTCAAGAAATTTATTCATGTATGTTATGCTGAAATTTTTAACAGAAAAAATACGATAAAAAATAAAATGAAGGTATCAAAGCGGTCCTTTTTAAAGCAAATATATGGACCAAAATGGACTCCTTGAAAGTAAGAAGACTAGAATGATCATTTTGAAGATTATAATCGTTATAATGTGTAAATGCACACCGCCTTTAAAGATTGTAGTCTTTCTATCTAGTTATTGGTTGAAGATCTTTTCTTAATCCACCTATGGTGAATGAGCATATCCCTTCCTCTTTTGTATGTCTCATTCATCAATGAAATTAAATCAGCTTCTCTAAAATGGATGTATTGAAAGTATAAAAATTTAAATAAACTAAAAGTAGAAAAACTAAAATGGTGTTTAAACCATTAAAAATCTACCAATGTTCTAACTAATTCAACACCTTTAAATTCAGTTTGATTGATGGATATGCTACACTTGCAAATCGATTGCCAGAAAGCAAATTATACTGCACCAATGATGAGTCAAAATATTTATAA

mRNA sequence

ATGTGCAAGAAGCCTCGATGGAATATCATGAAACTGAAAGGCCACATGGAGTACTTGGTACTGAAGTATCTTTCAATTAATGAGAAAGATGATATCCCCTGGGCTCTCATCCGGGCAGGATTATCTTCTGTGGCACAAACTGCCATAATACCTTTGCAAGATGTTCTTGGGTTAGGAACTTCTGCAAGGATGAACATTCCAGCAACACAGTTTGGAAACTGGAAATGGAGAATACCTAGTTCCATAAGCTTTGAGAGTTTGGAGACAGAAGCTACTAAATTGAAGGATATGGTTTCATTGTATGGGCGGCTTTTGATTGATGGATATGCTACACTTGCAAATCGATTGCCAGAAAGCAAATTATACTGCACCAATGATGAGTCAAAATATTTATAA

Coding sequence (CDS)

ATGTGCAAGAAGCCTCGATGGAATATCATGAAACTGAAAGGCCACATGGAGTACTTGGTACTGAAGTATCTTTCAATTAATGAGAAAGATGATATCCCCTGGGCTCTCATCCGGGCAGGATTATCTTCTGTGGCACAAACTGCCATAATACCTTTGCAAGATGTTCTTGGGTTAGGAACTTCTGCAAGGATGAACATTCCAGCAACACAGTTTGGAAACTGGAAATGGAGAATACCTAGTTCCATAAGCTTTGAGAGTTTGGAGACAGAAGCTACTAAATTGAAGGATATGGTTTCATTGTATGGGCGGCTTTTGATTGATGGATATGCTACACTTGCAAATCGATTGCCAGAAAGCAAATTATACTGCACCAATGATGAGTCAAAATATTTATAA

Protein sequence

MCKKPRWNIMKLKGHMEYLVLKYLSINEKDDIPWALIRAGLSSVAQTAIIPLQDVLGLGTSARMNIPATQFGNWKWRIPSSISFESLETEATKLKDMVSLYGRLLIDGYATLANRLPESKLYCTNDESKYL

Homology

[Close]

BLAST of Lag0008018 vs. NCBI nr
Match: XP_038901900.1 (4-alpha-glucanotransferase, chloroplastic/amyloplastic isoform X4 [Benincasa hispida])

HSP 1 Score: 162.2 bits (409), Expect = 3.1e-36
Identity = 78/85 (91.76%), Postives = 83/85 (97.65%), Query Frame = 0


Query: 20  VLKYLSINEKDDIPWALIRAGLSSVAQTAIIPLQDVLGLGTSARMNIPATQFGNWKWRIP 79
           VLKYLS+ EKDDIPWALIRA LSSVAQTAIIPLQDVLGLG+SARMNIPATQFGNWKWRIP
Sbjct: 494 VLKYLSVTEKDDIPWALIRAALSSVAQTAIIPLQDVLGLGSSARMNIPATQFGNWKWRIP 553

Query: 80  SSISFESLETEATKLKDMVSLYGRL 105
           SS+SFESLETEA+KLKDM+SLYGRL
Sbjct: 554 SSVSFESLETEASKLKDMISLYGRL 578

[Close]

BLAST of Lag0008018 vs. NCBI nr
Match: XP_038901901.1 (4-alpha-glucanotransferase, chloroplastic/amyloplastic isoform X5 [Benincasa hispida])

HSP 1 Score: 162.2 bits (409), Expect = 3.1e-36
Identity = 78/85 (91.76%), Postives = 83/85 (97.65%), Query Frame = 0


Query: 20  VLKYLSINEKDDIPWALIRAGLSSVAQTAIIPLQDVLGLGTSARMNIPATQFGNWKWRIP 79
           VLKYLS+ EKDDIPWALIRA LSSVAQTAIIPLQDVLGLG+SARMNIPATQFGNWKWRIP
Sbjct: 493 VLKYLSVTEKDDIPWALIRAALSSVAQTAIIPLQDVLGLGSSARMNIPATQFGNWKWRIP 552

Query: 80  SSISFESLETEATKLKDMVSLYGRL 105
           SS+SFESLETEA+KLKDM+SLYGRL
Sbjct: 553 SSVSFESLETEASKLKDMISLYGRL 577

[Close]

BLAST of Lag0008018 vs. NCBI nr
Match: XP_038901897.1 (4-alpha-glucanotransferase, chloroplastic/amyloplastic isoform X1 [Benincasa hispida])

HSP 1 Score: 162.2 bits (409), Expect = 3.1e-36
Identity = 78/85 (91.76%), Postives = 83/85 (97.65%), Query Frame = 0


Query: 20  VLKYLSINEKDDIPWALIRAGLSSVAQTAIIPLQDVLGLGTSARMNIPATQFGNWKWRIP 79
           VLKYLS+ EKDDIPWALIRA LSSVAQTAIIPLQDVLGLG+SARMNIPATQFGNWKWRIP
Sbjct: 562 VLKYLSVTEKDDIPWALIRAALSSVAQTAIIPLQDVLGLGSSARMNIPATQFGNWKWRIP 621

Query: 80  SSISFESLETEATKLKDMVSLYGRL 105
           SS+SFESLETEA+KLKDM+SLYGRL
Sbjct: 622 SSVSFESLETEASKLKDMISLYGRL 646

[Close]

BLAST of Lag0008018 vs. NCBI nr
Match: XP_038901898.1 (4-alpha-glucanotransferase, chloroplastic/amyloplastic isoform X2 [Benincasa hispida])

HSP 1 Score: 162.2 bits (409), Expect = 3.1e-36
Identity = 78/85 (91.76%), Postives = 83/85 (97.65%), Query Frame = 0


Query: 20  VLKYLSINEKDDIPWALIRAGLSSVAQTAIIPLQDVLGLGTSARMNIPATQFGNWKWRIP 79
           VLKYLS+ EKDDIPWALIRA LSSVAQTAIIPLQDVLGLG+SARMNIPATQFGNWKWRIP
Sbjct: 561 VLKYLSVTEKDDIPWALIRAALSSVAQTAIIPLQDVLGLGSSARMNIPATQFGNWKWRIP 620

Query: 80  SSISFESLETEATKLKDMVSLYGRL 105
           SS+SFESLETEA+KLKDM+SLYGRL
Sbjct: 621 SSVSFESLETEASKLKDMISLYGRL 645

[Close]

BLAST of Lag0008018 vs. NCBI nr
Match: XP_022154165.1 (4-alpha-glucanotransferase, chloroplastic/amyloplastic isoform X2 [Momordica charantia])

HSP 1 Score: 158.3 bits (399), Expect = 4.5e-35
Identity = 75/85 (88.24%), Postives = 83/85 (97.65%), Query Frame = 0


Query: 20  VLKYLSINEKDDIPWALIRAGLSSVAQTAIIPLQDVLGLGTSARMNIPATQFGNWKWRIP 79
           VLKYLSI E+DDIPWAL+R GLSSVAQTAIIPLQDVLGLG+SARMNIPATQFGNW+WRIP
Sbjct: 498 VLKYLSITEEDDIPWALVRTGLSSVAQTAIIPLQDVLGLGSSARMNIPATQFGNWRWRIP 557

Query: 80  SSISFESLETEATKLKDMVSLYGRL 105
           +S+SFESLETEATKLKD++SLYGRL
Sbjct: 558 NSLSFESLETEATKLKDLLSLYGRL 582

[Close]

BLAST of Lag0008018 vs. ExPASy Swiss-Prot
Match: Q9LV91 (4-alpha-glucanotransferase DPE1, chloroplastic/amyloplastic OS=Arabidopsis thaliana OX=3702 GN=DPE1 PE=1 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 4.3e-28
Identity = 57/84 (67.86%), Postives = 73/84 (86.90%), Query Frame = 0


Query: 21  LKYLSINEKDDIPWALIRAGLSSVAQTAIIPLQDVLGLGTSARMNIPATQFGNWKWRIPS 80
           +KYLSI  +DDI W++I+A  SS AQTAIIP+QD+LGLG+SARMN PAT+ GNW WRIPS
Sbjct: 493 MKYLSIAGEDDISWSVIQAAFSSTAQTAIIPMQDILGLGSSARMNTPATEVGNWGWRIPS 552

Query: 81  SISFESLETEATKLKDMVSLYGRL 105
           S SF++LETE+ +L+D++SLYGRL
Sbjct: 553 STSFDNLETESDRLRDLLSLYGRL 576

[Close]

BLAST of Lag0008018 vs. ExPASy Swiss-Prot
Match: Q06801 (4-alpha-glucanotransferase, chloroplastic/amyloplastic OS=Solanum tuberosum OX=4113 GN=DPEP PE=1 SV=1)

HSP 1 Score: 115.9 bits (289), Expect = 3.4e-25
Identity = 55/85 (64.71%), Postives = 70/85 (82.35%), Query Frame = 0


Query: 20  VLKYLSINEKDDIPWALIRAGLSSVAQTAIIPLQDVLGLGTSARMNIPATQFGNWKWRIP 79
           VLKYLS  E+++I   LI   +SSVA+ AIIP+QDVLGLG+ +RMNIPATQFGNW WRIP
Sbjct: 492 VLKYLSNIEEEEISRGLIEGAVSSVARIAIIPMQDVLGLGSDSRMNIPATQFGNWSWRIP 551

Query: 80  SSISFESLETEATKLKDMVSLYGRL 105
           SS SF++L+ EA KL+D+++ YGRL
Sbjct: 552 SSTSFDNLDAEAKKLRDILATYGRL 576

[Close]

BLAST of Lag0008018 vs. ExPASy Swiss-Prot
Match: Q8LI30 (4-alpha-glucanotransferase DPE1, chloroplastic/amyloplastic OS=Oryza sativa subsp. japonica OX=39947 GN=DPE1 PE=2 SV=2)

HSP 1 Score: 110.9 bits (276), Expect = 1.1e-23
Identity = 51/85 (60.00%), Postives = 67/85 (78.82%), Query Frame = 0


Query: 20  VLKYLSINEKDDIPWALIRAGLSSVAQTAIIPLQDVLGLGTSARMNIPATQFGNWKWRIP 79
           V KYL    + +I WALI A LSSVA+T+++ +QD+LGL +SARMN PATQ GNW+WR+P
Sbjct: 510 VFKYLPEANRTEISWALITAALSSVARTSMVTMQDILGLDSSARMNTPATQKGNWRWRMP 569

Query: 80  SSISFESLETEATKLKDMVSLYGRL 105
           SS+SF+SL  EA KLK+++ LY RL
Sbjct: 570 SSVSFDSLSPEAAKLKELLGLYNRL 594

[Close]

BLAST of Lag0008018 vs. ExPASy Swiss-Prot
Match: O87172 (4-alpha-glucanotransferase OS=Thermus thermophilus OX=274 GN=malQ PE=1 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 1.2e-11
Identity = 32/69 (46.38%), Postives = 46/69 (66.67%), Query Frame = 0


Query: 18  YLVLKYLSINEKDDIPWALIRAGLSSVAQTAIIPLQDVLGLGTSARMNIPATQFGNWKWR 77
           YL    ++  E++++PWAL+  G+ SVA+ A+ P+QDVL LG+ ARMN P    GNW WR
Sbjct: 417 YLADWGITFREEEEVPWALMHLGMKSVARLAVYPVQDVLALGSEARMNYPGRPSGNWAWR 476

Query: 78  -IPSSISFE 86
            +P  +S E
Sbjct: 477 LLPGELSPE 485

[Close]

BLAST of Lag0008018 vs. ExPASy Swiss-Prot
Match: P72785 (4-alpha-glucanotransferase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=malQ PE=3 SV=1)

HSP 1 Score: 70.1 bits (170), Expect = 2.1e-11
Identity = 38/85 (44.71%), Postives = 49/85 (57.65%), Query Frame = 0


Query: 20  VLKYLSINEKDDIPWALIRAGLSSVAQTAIIPLQDVLGLGTSARMNIPATQFGNWKWRI- 79
           V+ YL     + I W+LIR   SSVA  AI PLQD+LGLG+  RMN+P T  GNW WR  
Sbjct: 419 VINYLGCVCNEGIHWSLIRLASSSVAALAIFPLQDILGLGSDCRMNLPGTAAGNWGWRYH 478

Query: 80  PSSISFESLETEATKLKDMVSLYGR 104
           P  ++    +  +  L  +  LYGR
Sbjct: 479 PDQLN----DWLSGHLSFITELYGR 499

The following BLAST results are available for this feature:

BLAST of Lag0008018 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038901900.1	3.1e-36	91.76	4-alpha-glucanotransferase, chloroplastic/amyloplastic isoform X4 [Benincasa his...	[more]
XP_038901901.1	3.1e-36	91.76	4-alpha-glucanotransferase, chloroplastic/amyloplastic isoform X5 [Benincasa his...	[more]
XP_038901897.1	3.1e-36	91.76	4-alpha-glucanotransferase, chloroplastic/amyloplastic isoform X1 [Benincasa his...	[more]
XP_038901898.1	3.1e-36	91.76	4-alpha-glucanotransferase, chloroplastic/amyloplastic isoform X2 [Benincasa his...	[more]
XP_022154165.1	4.5e-35	88.24	4-alpha-glucanotransferase, chloroplastic/amyloplastic isoform X2 [Momordica cha...	[more]

BLAST of Lag0008018 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LV91	4.3e-28	67.86	4-alpha-glucanotransferase DPE1, chloroplastic/amyloplastic OS=Arabidopsis thali...	[more]
Q06801	3.4e-25	64.71	4-alpha-glucanotransferase, chloroplastic/amyloplastic OS=Solanum tuberosum OX=4...	[more]
Q8LI30	1.1e-23	60.00	4-alpha-glucanotransferase DPE1, chloroplastic/amyloplastic OS=Oryza sativa subs...	[more]
O87172	1.2e-11	46.38	4-alpha-glucanotransferase OS=Thermus thermophilus OX=274 GN=malQ PE=1 SV=1	[more]
P72785	2.1e-11	44.71	4-alpha-glucanotransferase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=11...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003385	Glycoside hydrolase, family 77	PFAM	PF02446	Glyco_hydro_77	coord: 21..79 e-value: 1.3E-13 score: 50.6
IPR003385	Glycoside hydrolase, family 77	PANTHER	PTHR32438	4-ALPHA-GLUCANOTRANSFERASE DPE1, CHLOROPLASTIC/AMYLOPLASTIC	coord: 12..104
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 7..104 e-value: 4.1E-23 score: 83.7
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 17..103

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0008018.1	Lag0008018.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005977	glycogen metabolic process
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004134	4-alpha-glucanotransferase activity
molecular_function	GO:0102500	beta-maltose 4-alpha-glucanotransferase activity