Lag0008017 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0008017
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	4-alpha-glucanotransferase
Location	chr9: 10058171 .. 10063923 (+)
RNA-Seq Expression	Lag0008017
Synteny	Lag0008017

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGATCTCAGCCTCACTCCTCCCCTTTCCTCTGACTCCCAAGCTTCGGAATCCCCATTGTCGCCGCCCTGCCTCTCAACGCCACTACTACGTCCCTGTCCACCAGGAGATTCCAGCTCTTGTCATCTTCTTCCTCTACCCACAGTCTGAAAGTTGGCACCATTTTCGATACAGTTACTGTGGGGGAGGATTTTCCGGTCGACTATGGCGAATGGTTTCCGGAGACGGATCCTGACTCTCGGCGAAGAGCAGGTGTTCTGCTACATCCTACGTCGTTTCGAGGACCTTATGGCATTGGTGATCTTGGAGATGAGGCGTTTAGATTTATCGACTGGCTTCATGATGCGGGATTCTCCGTTTGGCAGGTTTGATTCTTACCACTCGTCTCTTGATTGCATTGGAAGTGGGAAAGTTTTGATGTTCTATTTCCCGTTGTTATAGGCTTGTAGTTGATTATTTGAATAAGTAATGGGGCCATTGAAGCAATTTTTGCAATGCATAAGTACTGCAATTCTTGAATTTATGAAGCAGTGCTCCGTAGATGGTTATATTTATGAATTTCCACTCGAGTGGCATTTCCCCTGTTCCTCTTCATGAATTTCCTATCTGATTGAATTCCCCATAAGTTCAGTCGTCCAGCGAGGAGCAAGAACAATACATTATGGCTTGCATGTCTTTCTTGCTTTGGTTGTTGGGAGTCCTGAGAAAAATGGAAATGAATGTAAACGGAGCCGAGACTTACCGGATTCCGTAGTGTTGGAATTAAAAGTCTAGTGCAATGGGGAAAATGCAAAGAAATTTGTTTATGTTATAGTTATATAGAATAAAGATGTGGAAATTTTGGAATCTGATCAATTTTGGTTGACTGATGCAACTGTGCAGGTTCTTCCTCTTGTTCCTCCAGGAAGGAAGGCTAATGAAGAGGGATCGCCCTACTCAGGCCAGGTGATGACTTTTCTGGTTAACCAATCTTTTAATTCTCCTTCTTCTCTCCTCCTCTCCTCTATCTTCTGTGTTTTCTTTCATCTTTCCTATGTTTGAGATGGGGATGGAACCGTGTGACTCATGAGGAAGTAAAGTTTACCACAATGTAGAGAATAGAGAATACACATTCAAAAATAAATTTACTTGGACGTCATCTCTATGAATACCAGGTTTGACCATTTGGGAACTGGTTGATGCATTTGGTTATTCGTATTCCCATCCCAATTATGTAGCCCCATTGGGTGCTCCATTGGCTAAGCCCGAACTCAGAGGTCGTGTGTTTGAATCTTCGGCTGAGCTGAAAACACTTGATGTCTCTGAAGTCTAGGCCTTACGGCAGGTGTGAATTTTTTTAAAAAGTATGTATTGGCTCGAATTCGGCATTCATTACCAGCATACTGAAAGGAAGGTGTGGCAAGACTCATATTACCCCTGGATTGCCTACTCATTGGAATTGCCACTTCTTACCCCATTTTTTCTGCAATTTTGAAATATTTTATTTATTAAAGATGAACTTCATATCACATCTAGTATTATCTGAATTAAAAAAATGAATGCATCAGAGATTTCTACTCATTGTTTAGAATTATAATGCCCCTTGAGATGGTTATTTCCTACACTAACCTCCAGCCATAAATGAAAGCATCAGAGATTTCTACTCATTATTTACAAAGTCCAGCTATGGCTGTATTTGAAGTTCCTTTTATTCGTCATGTATGATTTTCCTCTCCACAAATTGTAAAAGCAGAAATGGGGATAGATGCATTCAGGTGGTGGGGCTCATACGGCGGATCAAGCATAAAACCTGCCAATAGAAAGGTTGTTTGCTGGAGGACCTTCTATACATAATATAGGTCTTTTACTCCCAAATTAGGAGGTGAACATGAATAATTGTGATTAGTGGTCAATGGTGTGTTTTTAGGGGGTTTGTGCTAGTATATATTGTTCAGATCCTGTTAATGATTGTTTATACTCCTTGGACATTCGTCATTCTGAAAATAGCATGGTTGTTTCCTTCATTCCCTCATTAAGCTGACTTTCTGTGCTTGTTATGTTGCCTGTTTATTTTTATGAGACTCTTGGTGTGTCTGGTTGTAATAGATCATGTCGCTCAATGATGGAGGTGAGTGAACTCAAAAGAAAAAATAGATCTTTTGGTTGACTGTCAGCCCTTTGGATAGGGTGGCTATCTGAATGTGTCCCATGACCAGTTTTTGGTAATGGGTACACTCATGGTGTTGTATCACCCACCATTCTGAGCCAAGTGAGAGATCCTGTGGTGCGCAATCTTTTTCCTCCTATATCATGGAATATTTGACTCAGAAAAAATTGGAGAATTTTTAGAGGGGTTAACCATTGGAGGAAGGATGATTTATGGCTAGGTTTCTTGTCTCTTTTTGGGTGTTTATTTCTAAGAGTTTTTGTAGCTATTTCTTATCTCTTATGCCTAGCAGTGGAAGCCTCTTTTTGTGATCTTTGGGTTTATTTTATTTTATTTATTTTTTTATTTTGTACAAGCTCCTTGGGCTCCTTTTTGTGGGTTTCTACTTTTGACAAATTACTAAATTTTTTTATTGCCCAATTTGTATTCATTTATCTTAATGGAAGCTTGGTTTTAGATTAGAAAAATGTTTATTTATACGATCAATTTCAACAATTCTATGATGTGTTTACTTTTACCTCATTTGCATTGTTTTTTTATTGTTTCTGGTGAAAACTTTCAGGATGCAAATTGCGGAAACACTCTCTTGATCTCTCTTGATGAACTTGTCAAGGATGGTTTGCTAACAAAAGACGAGTTGCCAAAACCAGTGTAAGAAACTTTACTTAGATTAATTGTTAGCATCCCTTTATAATCTCCCGTTAAGGGACACTGGAGTAATCCTGCCCCTGAATGGGTTTATTTGCAGCGACCCTGACCATGTAAACTTCTCTGCTGTTGCTGATATCAAGGATCCCTTGATAGCAAAGGTTAGTAATTAATCTTTCCCCTATCTGCTCTCATGAATGGCTGCATGTATTCAAGTATTGCTTATATGAGTGACTTGAAAATAAATTTCAACTTCGAATCTGTCTCCTAAATTTCCTCCATTTTTGAAAGTTACCATTATTAGTTATGGACAGGAAATTGTGTGTGTGTGTGTTAGAAGTTAGATTGTATTTAAAAAGAAAGGAGAAACAACCTAATGGTTAGGGGTACCCTCTATGAGAAAACAGCTATTACAGAAAACCTCTCCAATCCAAATTAATCGTAGAAAATATGATGTGATTACAGTGAAGCTAATTGTGAAGGGAAGTTCAACAAGAGGCTGTATACTATGCAAGCTCACCACGAAGAACCTATGGTTTCTTTCAAGCCATAGAACCCACAGAAAAGATCAAACTATACATTCCTCCATAGCACTTTAGCTTTCTCTTTCTGCCACAAATACGTTAAAGCTTTGTGAAGCCAAATATCTCTCGTTTTTTGGAGAGCAGTGGACATTTAAAGGTGGGGGGAGGGGGAGGGGGGGGGGGGGGTTTCCCTCATCCCCAGCCCTTAGGTTGTATGTGATTATTTTTTTGAATTCAGCCATTCCCTTGTTTCTTATCAAAAAAGAAAAAAAAAAGTGGACATTTAAAGGTGTCATAAATAAGACCATCCTCCAAAAGCAAAAGAATAGTTTGAGAAGAAATGGTGAAGGGATTCAGAATTTTAAAACCTATGTTTGAAGTTTTACACAGTTGTTAAAGTTCAAGTGTGGACTTACCTAAGCTGACAAAATAAACTTTTAGAGGTGCCACCTTGTTTCACTTTCATCATTGGGGGAAACAATGTGCTATCAATGCAAAATGTAAAGAGAAGTAAAACACATATAGTGCGTTAGATTGAGAGGGAACTATCTTATGTTAACATTCCAGTGTATATTGCTTCATGATCCCAATACAGATTTGCTAGCACTCTGATAACTTTCTATTCTGTAGTTGTTTTTTTTGGGGGTATTAATATGACTTGTGAATGTCTTTAATGTATTTGTTAATGTGGACAAATGGTTCCTTTGGTTCTGTCTTAGGCTGCAGAAAGGTTAATTCAAAGTGACGGTGAACTGAAAAGACAGCTTGAAGAATTTCGTAGAGACCCTGATATATCAAGTTAGTTTTTAGTATAATTGAAATGATTATATTCTTTTTTTTGGATCATCTCGTTGATTGTTAAAGAATTATACATGCTGTATTATCTTGGCCACCTATTTTATCCTTTCTACAAACTTACTAGGGCATCAAGTTGGCGAAAGAATTGTTTTGGTTGTGTGTGTGTGAGACAATAAATCACTTTTACTTTAATTTTTTTCTAAGATCTGTCAGTTTATTTAAAGTCCCAATTACTTCAAGAAAAGGCGTTCAAATTAGACTGGTAACCTCCTCAGTGACTGGATCTGAAAAGTGGTTTTACATCTACACCAATCCATAGCATGAAAGATAATAAAATCCATAAAACTATCAAAAGTAGGAAAGAGTCCCTGAAATTTCTTCCATTGATTATAGACTTACTGGTTACTACGTTGATTTCAAATTGAAGATCTTTCTTGTAATTCACCTTTAGCTGTTGAGAGTTTTTACTCCGTTATTTCATTCATTAACGAAATTTTTTCTCTTCAAAAAAATGACGGGATCTGGTCGGCTGTAGATTTGGTATCTGCTACCAAAATTCTGTTATGCTGAAGCTGAGCTGCCAAATTTACTCCAGCATGAATCCTTTTATGAATCTGTTTGTTAATACATATTTAATTGTCAAGTGTTTCCTCTAGTATAGGCCAACATAGATACTTCAAAAGCTTTCATTTATAAATTTTCTTCGTCACTGTTGGATGTAGAAGAAGAAAAAGAGAAAAAAGAGAATCAAAGACTATGAGATGGTTGTCCAAAATGATTCTAGTTGGTACCAATACTTACTTGGGTAGTCATCCCCTAGTTTTCTGTTTTACTGAATGGCAGCTCAAATATACTTGTGTTAGATTGACTATCCAATAAAGTCTGCTGGTTGATTAATTAATCATCTAACTTACCAGTTTAGAACAGCTCTTTGTATTTCCTTTCACTCTTCCTCTCGTTCTTCTTGTTCAATTTTTCAGGCTGGCTTGAAGATGCAGCTTATTTTGCTGCTATTGACAATAGACTAAATTATTTCAGTTGGTATGAATGGCCTGAACCCTTAAAAAACCGGCACCTTTCAGCTTTAGAAGATGTTTACCAAAGGGAAAGAGATTTCGTAAGATTTGGGTGAATTCTTTAGTTCAACTTTTGAGCTTATAGTTTCTGTTGTCTGTAACCTAATTGCTCATGAGTACTTTTGTTTTTGCAGATAAATATATTCATTGCTCAACAGTTTTTATTCCAGAGACAATGGCAAAGAGTTCGCAGCTACGCAAATATGAAGGGTATTACTATAATGGGAGACATGCCTATTTATGTTGGCTATCAGAGTGCAGATGTTTGGGCTAATAAGAAGCAATTCTTGCTGGTATATATTGCTTCTGAATGAGCAGAGACGGAAGAAGATATACTATGTAATGTTTTCAGTACGTTGACTGCTATTGCTTCTTTTGGCAGAACAAGAAAGGTTTTCCTGTGTTAGTGAGTGGTGTGCCTCCAGATGCATTCAGTGAAACTGGTCAACTATGGGGAAGGTGTGTACTGTACTTATCTCATTCAAATGGTGGATTGCACATTCTGTTTTCTGATGAAATATGTTTTCTATTTTTCCGTAGTTATTACATAAGGTCATAG

mRNA sequence

ATGGCGATCTCAGCCTCACTCCTCCCCTTTCCTCTGACTCCCAAGCTTCGGAATCCCCATTGTCGCCGCCCTGCCTCTCAACGCCACTACTACGTCCCTGTCCACCAGGAGATTCCAGCTCTTGTCATCTTCTTCCTCTACCCACAGTCTGAAAGTTGGCACCATTTTCGATACAGTGTTCTGCTACATCCTACGTCGTTTCGAGGACCTTATGGCATTGGTGATCTTGGAGATGAGGCGTTTAGATTTATCGACTGGCTTCATGATGCGGGATTCTCCGTTTGGCAGGTTCTTCCTCTTGTTCCTCCAGGAAGGAAGGCTAATGAAGAGGGATCGCCCTACTCAGGCCAGGATGCAAATTGCGGAAACACTCTCTTGATCTCTCTTGATGAACTTGTCAAGGATGGTTTGCTAACAAAAGACGAGTTGCCAAAACCAGTCGACCCTGACCATGTAAACTTCTCTGCTGTTGCTGATATCAAGGATCCCTTGATAGCAAAGGCTGCAGAAAGGTTAATTCAAAGTGACGGTGAACTGAAAAGACAGCTTGAAGAATTTCGTAGAGACCCTGATATATCAAGCTGGCTTGAAGATGCAGCTTATTTTGCTGCTATTGACAATAGACTAAATTATTTCAGTTGGTATGAATGGCCTGAACCCTTAAAAAACCGGCACCTTTCAGCTTTAGAAGATGTTTACCAAAGGGAAAGAGATTTCATAAATATATTCATTGCTCAACAGTTTTTATTCCAGAGACAATGGCAAAGAGTTCGCAGCTACGCAAATATGAAGGGTATTACTATAATGGGAGACATGCCTATTTATGTTGGCTATCAGAGTGCAGATGTTTGGGCTAATAAGAAGCAATTCTTGCTGAACAAGAAAGGTTTTCCTGTGTTAGTGAGTGGTGTGCCTCCAGATGCATTCAGTGAAACTGGTCAACTATGGGGAAGTTATTACATAAGGTCATAG

Coding sequence (CDS)

ATGGCGATCTCAGCCTCACTCCTCCCCTTTCCTCTGACTCCCAAGCTTCGGAATCCCCATTGTCGCCGCCCTGCCTCTCAACGCCACTACTACGTCCCTGTCCACCAGGAGATTCCAGCTCTTGTCATCTTCTTCCTCTACCCACAGTCTGAAAGTTGGCACCATTTTCGATACAGTGTTCTGCTACATCCTACGTCGTTTCGAGGACCTTATGGCATTGGTGATCTTGGAGATGAGGCGTTTAGATTTATCGACTGGCTTCATGATGCGGGATTCTCCGTTTGGCAGGTTCTTCCTCTTGTTCCTCCAGGAAGGAAGGCTAATGAAGAGGGATCGCCCTACTCAGGCCAGGATGCAAATTGCGGAAACACTCTCTTGATCTCTCTTGATGAACTTGTCAAGGATGGTTTGCTAACAAAAGACGAGTTGCCAAAACCAGTCGACCCTGACCATGTAAACTTCTCTGCTGTTGCTGATATCAAGGATCCCTTGATAGCAAAGGCTGCAGAAAGGTTAATTCAAAGTGACGGTGAACTGAAAAGACAGCTTGAAGAATTTCGTAGAGACCCTGATATATCAAGCTGGCTTGAAGATGCAGCTTATTTTGCTGCTATTGACAATAGACTAAATTATTTCAGTTGGTATGAATGGCCTGAACCCTTAAAAAACCGGCACCTTTCAGCTTTAGAAGATGTTTACCAAAGGGAAAGAGATTTCATAAATATATTCATTGCTCAACAGTTTTTATTCCAGAGACAATGGCAAAGAGTTCGCAGCTACGCAAATATGAAGGGTATTACTATAATGGGAGACATGCCTATTTATGTTGGCTATCAGAGTGCAGATGTTTGGGCTAATAAGAAGCAATTCTTGCTGAACAAGAAAGGTTTTCCTGTGTTAGTGAGTGGTGTGCCTCCAGATGCATTCAGTGAAACTGGTCAACTATGGGGAAGTTATTACATAAGGTCATAG

Protein sequence

MAISASLLPFPLTPKLRNPHCRRPASQRHYYVPVHQEIPALVIFFLYPQSESWHHFRYSVLLHPTSFRGPYGIGDLGDEAFRFIDWLHDAGFSVWQVLPLVPPGRKANEEGSPYSGQDANCGNTLLISLDELVKDGLLTKDELPKPVDPDHVNFSAVADIKDPLIAKAAERLIQSDGELKRQLEEFRRDPDISSWLEDAAYFAAIDNRLNYFSWYEWPEPLKNRHLSALEDVYQRERDFINIFIAQQFLFQRQWQRVRSYANMKGITIMGDMPIYVGYQSADVWANKKQFLLNKKGFPVLVSGVPPDAFSETGQLWGSYYIRS

Homology

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BLAST of Lag0008017 vs. NCBI nr
Match: XP_022154165.1 (4-alpha-glucanotransferase, chloroplastic/amyloplastic isoform X2 [Momordica charantia])

HSP 1 Score: 545.0 bits (1403), Expect = 4.2e-151
Identity = 275/346 (79.48%), Postives = 287/346 (82.95%), Query Frame = 0


Query: 1   MAISASLLPFPLTPKLRNPHCRRP--------------------ASQRHYYVPVHQEIPA 60
           MAIS SL PFPLTPKLRNP    P                    +S   + + VH    +
Sbjct: 1   MAISTSLFPFPLTPKLRNPPLSPPPLPLNASTATLSTRRFGPLSSSSSTHNLTVHAIFDS 60

Query: 61  LVI--------FFLYPQSESWHHFRYSVLLHPTSFRGPYGIGDLGDEAFRFIDWLHDAGF 120
           + +           +P++      R  VLLHPTSFRGPYGIGDLGDEAFRF+DWLH+AGF
Sbjct: 61  VGVGEDFPPHYGEWFPETNPDVRRRAGVLLHPTSFRGPYGIGDLGDEAFRFVDWLHEAGF 120

Query: 121 SVWQVLPLVPPGRKANEEGSPYSGQDANCGNTLLISLDELVKDGLLTKDELPKPVDPDHV 180
           SVWQVLPLVPPGRKANEEGSPYSGQDANCGNTLLISLDELVKDGLLTKDELPKPV+ DHV
Sbjct: 121 SVWQVLPLVPPGRKANEEGSPYSGQDANCGNTLLISLDELVKDGLLTKDELPKPVNHDHV 180

Query: 181 NFSAVADIKDPLIAKAAERLIQSDGELKRQLEEFRRDPDISSWLEDAAYFAAIDNRLNYF 240
           NFSAVADIKDPLIAKAAERLIQSDGELK QLEEF RDPDISSWLEDAAYFAAIDNRLN F
Sbjct: 181 NFSAVADIKDPLIAKAAERLIQSDGELKCQLEEFCRDPDISSWLEDAAYFAAIDNRLNCF 240

Query: 241 SWYEWPEPLKNRHLSALEDVYQRERDFINIFIAQQFLFQRQWQRVRSYANMKGITIMGDM 300
           SWYEWPEPLKNRHLSALEDVYQRERDFINIFIAQQFLFQRQWQRVRSYANMKGITIMGDM
Sbjct: 241 SWYEWPEPLKNRHLSALEDVYQRERDFINIFIAQQFLFQRQWQRVRSYANMKGITIMGDM 300

Query: 301 PIYVGYQSADVWANKKQFLLNKKGFPVLVSGVPPDAFSETGQLWGS 319
           PIYVGYQSADVWANKKQFLLNKKGFPVLVSGVPPDAFSETGQLWGS
Sbjct: 301 PIYVGYQSADVWANKKQFLLNKKGFPVLVSGVPPDAFSETGQLWGS 346

[Close]

BLAST of Lag0008017 vs. NCBI nr
Match: XP_022154164.1 (4-alpha-glucanotransferase, chloroplastic/amyloplastic isoform X1 [Momordica charantia])

HSP 1 Score: 545.0 bits (1403), Expect = 4.2e-151
Identity = 275/346 (79.48%), Postives = 287/346 (82.95%), Query Frame = 0


Query: 1   MAISASLLPFPLTPKLRNPHCRRP--------------------ASQRHYYVPVHQEIPA 60
           MAIS SL PFPLTPKLRNP    P                    +S   + + VH    +
Sbjct: 1   MAISTSLFPFPLTPKLRNPPLSPPPLPLNASTATLSTRRFGPLSSSSSTHNLTVHAIFDS 60

Query: 61  LVI--------FFLYPQSESWHHFRYSVLLHPTSFRGPYGIGDLGDEAFRFIDWLHDAGF 120
           + +           +P++      R  VLLHPTSFRGPYGIGDLGDEAFRF+DWLH+AGF
Sbjct: 61  VGVGEDFPPHYGEWFPETNPDVRRRAGVLLHPTSFRGPYGIGDLGDEAFRFVDWLHEAGF 120

Query: 121 SVWQVLPLVPPGRKANEEGSPYSGQDANCGNTLLISLDELVKDGLLTKDELPKPVDPDHV 180
           SVWQVLPLVPPGRKANEEGSPYSGQDANCGNTLLISLDELVKDGLLTKDELPKPV+ DHV
Sbjct: 121 SVWQVLPLVPPGRKANEEGSPYSGQDANCGNTLLISLDELVKDGLLTKDELPKPVNHDHV 180

Query: 181 NFSAVADIKDPLIAKAAERLIQSDGELKRQLEEFRRDPDISSWLEDAAYFAAIDNRLNYF 240
           NFSAVADIKDPLIAKAAERLIQSDGELK QLEEF RDPDISSWLEDAAYFAAIDNRLN F
Sbjct: 181 NFSAVADIKDPLIAKAAERLIQSDGELKCQLEEFCRDPDISSWLEDAAYFAAIDNRLNCF 240

Query: 241 SWYEWPEPLKNRHLSALEDVYQRERDFINIFIAQQFLFQRQWQRVRSYANMKGITIMGDM 300
           SWYEWPEPLKNRHLSALEDVYQRERDFINIFIAQQFLFQRQWQRVRSYANMKGITIMGDM
Sbjct: 241 SWYEWPEPLKNRHLSALEDVYQRERDFINIFIAQQFLFQRQWQRVRSYANMKGITIMGDM 300

Query: 301 PIYVGYQSADVWANKKQFLLNKKGFPVLVSGVPPDAFSETGQLWGS 319
           PIYVGYQSADVWANKKQFLLNKKGFPVLVSGVPPDAFSETGQLWGS
Sbjct: 301 PIYVGYQSADVWANKKQFLLNKKGFPVLVSGVPPDAFSETGQLWGS 346

[Close]

BLAST of Lag0008017 vs. NCBI nr
Match: XP_038901901.1 (4-alpha-glucanotransferase, chloroplastic/amyloplastic isoform X5 [Benincasa hispida])

HSP 1 Score: 541.2 bits (1393), Expect = 6.1e-150
Identity = 277/342 (80.99%), Postives = 286/342 (83.63%), Query Frame = 0


Query: 1   MAISASLLPFPLTPKLRNP-----------HCRR-----PASQRHYY--------VPVHQ 60
           MAIS S+ PF LTPKLRNP             RR      +S  H          V V +
Sbjct: 1   MAISTSVFPFHLTPKLRNPPLPLYATTTFLSTRRFQHSSSSSSTHTLKVSTIFDTVTVGE 60

Query: 61  EIPALVIFFLYPQSESWHHFRYSVLLHPTSFRGPYGIGDLGDEAFRFIDWLHDAGFSVWQ 120
           + P     + +P S+     R  VLLHPTSFRGPYGIGDLGDEAFRFIDWLH+AGFSVWQ
Sbjct: 61  DFPVHYGEW-FPDSDPDIRRRAGVLLHPTSFRGPYGIGDLGDEAFRFIDWLHEAGFSVWQ 120

Query: 121 VLPLVPPGRKANEEGSPYSGQDANCGNTLLISLDELVKDGLLTKDELPKPVDPDHVNFSA 180
           VLPLVPPGRKANEEGSPYSGQDANCGNTLLISLDELVKDGLLTKDELPKPVD DHVNFS 
Sbjct: 121 VLPLVPPGRKANEEGSPYSGQDANCGNTLLISLDELVKDGLLTKDELPKPVDHDHVNFSV 180

Query: 181 VADIKDPLIAKAAERLIQSDGELKRQLEEFRRDPDISSWLEDAAYFAAIDNRLNYFSWYE 240
           VADIKDPLIAKAAERLIQSDGELKRQLEEF RDPDISSWLEDAAYFAAIDNRLN FSWYE
Sbjct: 181 VADIKDPLIAKAAERLIQSDGELKRQLEEFCRDPDISSWLEDAAYFAAIDNRLNCFSWYE 240

Query: 241 WPEPLKNRHLSALEDVYQRERDFINIFIAQQFLFQRQWQRVRSYANMKGITIMGDMPIYV 300
           WPEPLKNRHLSALEDVYQRERDFINIFIAQQFLFQRQW+RVRSYAN KGITIMGDMPIYV
Sbjct: 241 WPEPLKNRHLSALEDVYQRERDFINIFIAQQFLFQRQWRRVRSYANTKGITIMGDMPIYV 300

Query: 301 GYQSADVWANKKQFLLNKKGFPVLVSGVPPDAFSETGQLWGS 319
           GYQSADVWANKKQFLLNKKGFPVLVSGVPPDAFSETGQLWGS
Sbjct: 301 GYQSADVWANKKQFLLNKKGFPVLVSGVPPDAFSETGQLWGS 341

[Close]

BLAST of Lag0008017 vs. NCBI nr
Match: XP_038901898.1 (4-alpha-glucanotransferase, chloroplastic/amyloplastic isoform X2 [Benincasa hispida])

HSP 1 Score: 541.2 bits (1393), Expect = 6.1e-150
Identity = 277/342 (80.99%), Postives = 286/342 (83.63%), Query Frame = 0


Query: 1   MAISASLLPFPLTPKLRNP-----------HCRR-----PASQRHYY--------VPVHQ 60
           MAIS S+ PF LTPKLRNP             RR      +S  H          V V +
Sbjct: 1   MAISTSVFPFHLTPKLRNPPLPLYATTTFLSTRRFQHSSSSSSTHTLKVSTIFDTVTVGE 60

Query: 61  EIPALVIFFLYPQSESWHHFRYSVLLHPTSFRGPYGIGDLGDEAFRFIDWLHDAGFSVWQ 120
           + P     + +P S+     R  VLLHPTSFRGPYGIGDLGDEAFRFIDWLH+AGFSVWQ
Sbjct: 61  DFPVHYGEW-FPDSDPDIRRRAGVLLHPTSFRGPYGIGDLGDEAFRFIDWLHEAGFSVWQ 120

Query: 121 VLPLVPPGRKANEEGSPYSGQDANCGNTLLISLDELVKDGLLTKDELPKPVDPDHVNFSA 180
           VLPLVPPGRKANEEGSPYSGQDANCGNTLLISLDELVKDGLLTKDELPKPVD DHVNFS 
Sbjct: 121 VLPLVPPGRKANEEGSPYSGQDANCGNTLLISLDELVKDGLLTKDELPKPVDHDHVNFSV 180

Query: 181 VADIKDPLIAKAAERLIQSDGELKRQLEEFRRDPDISSWLEDAAYFAAIDNRLNYFSWYE 240
           VADIKDPLIAKAAERLIQSDGELKRQLEEF RDPDISSWLEDAAYFAAIDNRLN FSWYE
Sbjct: 181 VADIKDPLIAKAAERLIQSDGELKRQLEEFCRDPDISSWLEDAAYFAAIDNRLNCFSWYE 240

Query: 241 WPEPLKNRHLSALEDVYQRERDFINIFIAQQFLFQRQWQRVRSYANMKGITIMGDMPIYV 300
           WPEPLKNRHLSALEDVYQRERDFINIFIAQQFLFQRQW+RVRSYAN KGITIMGDMPIYV
Sbjct: 241 WPEPLKNRHLSALEDVYQRERDFINIFIAQQFLFQRQWRRVRSYANTKGITIMGDMPIYV 300

Query: 301 GYQSADVWANKKQFLLNKKGFPVLVSGVPPDAFSETGQLWGS 319
           GYQSADVWANKKQFLLNKKGFPVLVSGVPPDAFSETGQLWGS
Sbjct: 301 GYQSADVWANKKQFLLNKKGFPVLVSGVPPDAFSETGQLWGS 341

[Close]

BLAST of Lag0008017 vs. NCBI nr
Match: XP_038901900.1 (4-alpha-glucanotransferase, chloroplastic/amyloplastic isoform X4 [Benincasa hispida])

HSP 1 Score: 536.6 bits (1381), Expect = 1.5e-148
Identity = 277/343 (80.76%), Postives = 286/343 (83.38%), Query Frame = 0


Query: 1   MAISASLLPFPLTPKLRNP-----------HCRR-----PASQRHYY--------VPVHQ 60
           MAIS S+ PF LTPKLRNP             RR      +S  H          V V +
Sbjct: 1   MAISTSVFPFHLTPKLRNPPLPLYATTTFLSTRRFQHSSSSSSTHTLKVSTIFDTVTVGE 60

Query: 61  EIPALVIFFLYPQSESWHHFRYSVLLHPTSFRGPYGIGDLGDEAFRFIDWLHDAGFSVWQ 120
           + P     + +P S+     R  VLLHPTSFRGPYGIGDLGDEAFRFIDWLH+AGFSVWQ
Sbjct: 61  DFPVHYGEW-FPDSDPDIRRRAGVLLHPTSFRGPYGIGDLGDEAFRFIDWLHEAGFSVWQ 120

Query: 121 VLPLVPPGRKANEEGSPYSGQDANCGNTLLISLDELVKDGLLTKDELPKPV-DPDHVNFS 180
           VLPLVPPGRKANEEGSPYSGQDANCGNTLLISLDELVKDGLLTKDELPKPV D DHVNFS
Sbjct: 121 VLPLVPPGRKANEEGSPYSGQDANCGNTLLISLDELVKDGLLTKDELPKPVSDHDHVNFS 180

Query: 181 AVADIKDPLIAKAAERLIQSDGELKRQLEEFRRDPDISSWLEDAAYFAAIDNRLNYFSWY 240
            VADIKDPLIAKAAERLIQSDGELKRQLEEF RDPDISSWLEDAAYFAAIDNRLN FSWY
Sbjct: 181 VVADIKDPLIAKAAERLIQSDGELKRQLEEFCRDPDISSWLEDAAYFAAIDNRLNCFSWY 240

Query: 241 EWPEPLKNRHLSALEDVYQRERDFINIFIAQQFLFQRQWQRVRSYANMKGITIMGDMPIY 300
           EWPEPLKNRHLSALEDVYQRERDFINIFIAQQFLFQRQW+RVRSYAN KGITIMGDMPIY
Sbjct: 241 EWPEPLKNRHLSALEDVYQRERDFINIFIAQQFLFQRQWRRVRSYANTKGITIMGDMPIY 300

Query: 301 VGYQSADVWANKKQFLLNKKGFPVLVSGVPPDAFSETGQLWGS 319
           VGYQSADVWANKKQFLLNKKGFPVLVSGVPPDAFSETGQLWGS
Sbjct: 301 VGYQSADVWANKKQFLLNKKGFPVLVSGVPPDAFSETGQLWGS 342

[Close]

BLAST of Lag0008017 vs. ExPASy Swiss-Prot
Match: Q06801 (4-alpha-glucanotransferase, chloroplastic/amyloplastic OS=Solanum tuberosum OX=4113 GN=DPEP PE=1 SV=1)

HSP 1 Score: 449.5 bits (1155), Expect = 3.2e-125
Identity = 204/262 (77.86%), Postives = 236/262 (90.08%), Query Frame = 0


Query: 57  RYSVLLHPTSFRGPYGIGDLGDEAFRFIDWLHDAGFSVWQVLPLVPPGRKANEEGSPYSG 116
           R  +LLHPTSF GPYGIGDLG +AF+F+DWLH AG S+WQVLPLVPPG++ NE+GSPYSG
Sbjct: 79  RAGILLHPTSFPGPYGIGDLGPQAFKFLDWLHLAGCSLWQVLPLVPPGKRGNEDGSPYSG 138

Query: 117 QDANCGNTLLISLDELVKDGLLTKDELPKPVDPDHVNFSAVADIKDPLIAKAAERLIQSD 176
           QDANCGNTLLISL+ELV DGLL  +ELP+P+  D VN+S +++IKDPLI KAA+RL+ S+
Sbjct: 139 QDANCGNTLLISLEELVDDGLLKMEELPEPLPTDRVNYSTISEIKDPLITKAAKRLLSSE 198

Query: 177 GELKRQLEEFRRDPDISSWLEDAAYFAAIDNRLNYFSWYEWPEPLKNRHLSALEDVYQRE 236
           GELK QLE FRRDP+ISSWLEDAAYFAAIDN +N  SWY+WPEPLKNRHL+ALE+VYQ E
Sbjct: 199 GELKDQLENFRRDPNISSWLEDAAYFAAIDNSVNTISWYDWPEPLKNRHLAALEEVYQSE 258

Query: 237 RDFINIFIAQQFLFQRQWQRVRSYANMKGITIMGDMPIYVGYQSADVWANKKQFLLNKKG 296
           +DFI+IFIAQQFLFQRQW++VR YA  KGI+IMGDMPIYVGY SADVWANKKQFLLN+KG
Sbjct: 259 KDFIDIFIAQQFLFQRQWKKVRDYARSKGISIMGDMPIYVGYHSADVWANKKQFLLNRKG 318

Query: 297 FPVLVSGVPPDAFSETGQLWGS 319
           FP++VSGVPPDAFSETGQLWGS
Sbjct: 319 FPLIVSGVPPDAFSETGQLWGS 340

[Close]

BLAST of Lag0008017 vs. ExPASy Swiss-Prot
Match: Q9LV91 (4-alpha-glucanotransferase DPE1, chloroplastic/amyloplastic OS=Arabidopsis thaliana OX=3702 GN=DPE1 PE=1 SV=1)

HSP 1 Score: 427.6 bits (1098), Expect = 1.3e-118
Identity = 198/262 (75.57%), Postives = 224/262 (85.50%), Query Frame = 0


Query: 57  RYSVLLHPTSFRGPYGIGDLGDEAFRFIDWLHDAGFSVWQVLPLVPPGRKANEEGSPYSG 116
           R  VLLHPTSFRGP+GIGDLG+EAFRFIDWLH  G SVWQVLPLVPP    +E GSPY+G
Sbjct: 83  RAGVLLHPTSFRGPHGIGDLGEEAFRFIDWLHSTGCSVWQVLPLVPP----DEGGSPYAG 142

Query: 117 QDANCGNTLLISLDELVKDGLLTKDELPKPVDPDHVNFSAVADIKDPLIAKAAERLIQSD 176
           QDANCGNTLLISLDELVKDGLL KDELP+P+D D VN+     +K PLI KAA+RLI  +
Sbjct: 143 QDANCGNTLLISLDELVKDGLLIKDELPQPIDADSVNYQTANKLKSPLITKAAKRLIDGN 202

Query: 177 GELKRQLEEFRRDPDISSWLEDAAYFAAIDNRLNYFSWYEWPEPLKNRHLSALEDVYQRE 236
           GELK +L +FR DP IS WLEDAAYFAAIDN LN +SW+EWPEPLKNRHLSALE +Y+ +
Sbjct: 203 GELKSKLLDFRNDPSISCWLEDAAYFAAIDNTLNAYSWFEWPEPLKNRHLSALEAIYESQ 262

Query: 237 RDFINIFIAQQFLFQRQWQRVRSYANMKGITIMGDMPIYVGYQSADVWANKKQFLLNKKG 296
           ++FI++FIA+QFLFQRQWQ+VR YA  +G+ IMGDMPIYVGY SADVWANKK FLLNKKG
Sbjct: 263 KEFIDLFIAKQFLFQRQWQKVREYARRQGVDIMGDMPIYVGYHSADVWANKKHFLLNKKG 322

Query: 297 FPVLVSGVPPDAFSETGQLWGS 319
           FP+LVSGVPPD FSETGQLWGS
Sbjct: 323 FPLLVSGVPPDLFSETGQLWGS 340

[Close]

BLAST of Lag0008017 vs. ExPASy Swiss-Prot
Match: Q8LI30 (4-alpha-glucanotransferase DPE1, chloroplastic/amyloplastic OS=Oryza sativa subsp. japonica OX=39947 GN=DPE1 PE=2 SV=2)

HSP 1 Score: 419.1 bits (1076), Expect = 4.6e-116
Identity = 193/273 (70.70%), Postives = 227/273 (83.15%), Query Frame = 0


Query: 46  LYPQSESWHHFRYSVLLHPTSFRGPYGIGDLGDEAFRFIDWLHDAGFSVWQVLPLVPPGR 105
           + P  E     R  VLLHPTS RGP+GIGDLGDEA  F+ WL DAG ++WQVLPLVPPGR
Sbjct: 86  MMPAVEEARRRRAGVLLHPTSLRGPHGIGDLGDEAVAFLAWLRDAGCTLWQVLPLVPPGR 145

Query: 106 KANEEGSPYSGQDANCGNTLLISLDELVKDGLLTKDELPKPVDPDHVNFSAVADIKDPLI 165
           K+ E+GSPYSGQDANCGNTLLISL+ELVKDGLL ++ELP P+D ++V F  VA++K+PLI
Sbjct: 146 KSGEDGSPYSGQDANCGNTLLISLEELVKDGLLMENELPDPLDMEYVEFDTVANLKEPLI 205

Query: 166 AKAAERLIQSDGELKRQLEEFRRDPDISSWLEDAAYFAAIDNRLNYFSWYEWPEPLKNRH 225
           AKAAERL+ S GEL+ Q + F+++P+IS WLEDAA FAAID  ++  SWYEWPEPLKNRH
Sbjct: 206 AKAAERLLLSRGELRTQYDCFKKNPNISGWLEDAALFAAIDRSIDALSWYEWPEPLKNRH 265

Query: 226 LSALEDVYQRERDFINIFIAQQFLFQRQWQRVRSYANMKGITIMGDMPIYVGYQSADVWA 285
           L ALED+YQ+++DFI IF+AQQFLFQRQWQR+R YA   GI+IMGDMPIYVGY SADVWA
Sbjct: 266 LRALEDIYQKQKDFIEIFMAQQFLFQRQWQRIRKYAKKLGISIMGDMPIYVGYHSADVWA 325

Query: 286 NKKQFLLNKKGFPVLVSGVPPDAFSETGQLWGS 319
           N+K FLL+K GFP  VSGVPPDAFSETGQLW S
Sbjct: 326 NRKSFLLDKNGFPTFVSGVPPDAFSETGQLWNS 358

[Close]

BLAST of Lag0008017 vs. ExPASy Swiss-Prot
Match: O66937 (4-alpha-glucanotransferase OS=Aquifex aeolicus (strain VF5) OX=224324 GN=malQ PE=1 SV=1)

HSP 1 Score: 200.3 bits (508), Expect = 3.4e-50
Identity = 108/259 (41.70%), Postives = 153/259 (59.07%), Query Frame = 0


Query: 60  VLLHPTSFRGPYGIGDLGDEAFRFIDWLHDAGFSVWQVLPLVPPGRKANEEGSPYSGQDA 119
           +LLH TS   PYGIGDLG EA+RF+D+L + GFS+WQVLPL P   +A    SPYS    
Sbjct: 6   ILLHVTSLPSPYGIGDLGKEAYRFLDFLKECGFSLWQVLPLNPTSLEAG--NSPYSSNSL 65

Query: 120 NCGNTLLISLDELVKDGLLTKDELPKPVDPDHVNFSAVADIKDPLIAKAAERLIQSDGEL 179
             GN +LI  +EL+++ L+ + +L K        +  V + K  L+ KA +   + +   
Sbjct: 66  FAGNYVLIDPEELLEEDLIKERDL-KRFPLGEALYEVVYEYKKELLEKAFKNFRRFE--- 125

Query: 180 KRQLEEFRRDPDISSWLEDAAYFAAIDNRLNYFSWYEWPEPLKNRHLSALEDVYQRERDF 239
              LE+F ++   S WL D A + AI        WYEW E LK R   AL+ V  + +  
Sbjct: 126 --LLEDFLKEH--SYWLRDYALYMAIKEEEGK-EWYEWDEELKRREKEALKRVLNKLKGR 185

Query: 240 INIFIAQQFLFQRQWQRVRSYANMKGITIMGDMPIYVGYQSADVWANKKQFLLNKKGFPV 299
               +  QF+F +QW+++R YA  +GI+I+GD+P+Y  Y SADVW N + F L+    P+
Sbjct: 186 FYFHVFVQFVFFKQWEKLRRYARERGISIVGDLPMYPSYSSADVWTNPELFKLDGDLKPL 245

Query: 300 LVSGVPPDAFSETGQLWGS 319
            V+GVPPD FS+TGQLWG+
Sbjct: 246 FVAGVPPDFFSKTGQLWGN 253

[Close]

BLAST of Lag0008017 vs. ExPASy Swiss-Prot
Match: P72785 (4-alpha-glucanotransferase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=1111708 GN=malQ PE=3 SV=1)

HSP 1 Score: 182.6 bits (462), Expect = 7.2e-45
Identity = 109/264 (41.29%), Postives = 149/264 (56.44%), Query Frame = 0


Query: 60  VLLHPTSFRGPYGIGDLGDEAFRFIDWLHDAGFSVWQVLPLVPPGRKANEEGSPYSGQDA 119
           +LLHPTS    +GIGDLGD AF+FID+L DA  SVWQ+LPL P G       SPY    A
Sbjct: 9   ILLHPTSLPSRFGIGDLGDGAFQFIDFLADADQSVWQILPLGPTG----FGNSPYLCYSA 68

Query: 120 NCGNTLLISLDELVKDGLLTK---DELPKPVDPDHVNFSAVADIKDPLIAKA-AERLIQS 179
              N  LISLD L ++G L     D+ P   +P  V++      K  ++ +A A+     
Sbjct: 69  LAINPWLISLDRLAEEGFLPPSLLDQAPPFTNP-RVDYDQAIAYKSQVLKQAFAQFRTNI 128

Query: 180 DGELKRQLEEFRRDPDISSWLEDAAYFAAIDNRLNYFSWYEWPEPLKNRHLSALEDVYQR 239
           +  ++++  EF +    S WL D A F AI    N   W++W + +  R   AL+    R
Sbjct: 129 ELAIEQEFAEFCQAQ--SDWLADYALFMAIKEAHNGAGWHQWDKDIAWREPEALKIWGDR 188

Query: 240 ERDFINIFIAQQFLFQRQWQRVRSYANMKGITIMGDMPIYVGYQSADVWANKKQFLLN-K 299
            +  +      QFL  RQWQ V++YAN + I I GD+PIYV + SADVWAN + F L+ +
Sbjct: 189 LKTEVLYHQFLQFLGFRQWQEVKAYANQRHIAIFGDLPIYVAHDSADVWANPENFCLDPE 248

Query: 300 KGFPVLVSGVPPDAFSETGQLWGS 319
            G   +++GVPPD FS TGQLWG+
Sbjct: 249 TGEAAMMAGVPPDYFSATGQLWGN 265

[Close]

BLAST of Lag0008017 vs. ExPASy TrEMBL
Match: A0A6J1DL91 (4-alpha-glucanotransferase OS=Momordica charantia OX=3673 GN=LOC111021480 PE=3 SV=1)

HSP 1 Score: 545.0 bits (1403), Expect = 2.0e-151
Identity = 275/346 (79.48%), Postives = 287/346 (82.95%), Query Frame = 0


Query: 1   MAISASLLPFPLTPKLRNPHCRRP--------------------ASQRHYYVPVHQEIPA 60
           MAIS SL PFPLTPKLRNP    P                    +S   + + VH    +
Sbjct: 1   MAISTSLFPFPLTPKLRNPPLSPPPLPLNASTATLSTRRFGPLSSSSSTHNLTVHAIFDS 60

Query: 61  LVI--------FFLYPQSESWHHFRYSVLLHPTSFRGPYGIGDLGDEAFRFIDWLHDAGF 120
           + +           +P++      R  VLLHPTSFRGPYGIGDLGDEAFRF+DWLH+AGF
Sbjct: 61  VGVGEDFPPHYGEWFPETNPDVRRRAGVLLHPTSFRGPYGIGDLGDEAFRFVDWLHEAGF 120

Query: 121 SVWQVLPLVPPGRKANEEGSPYSGQDANCGNTLLISLDELVKDGLLTKDELPKPVDPDHV 180
           SVWQVLPLVPPGRKANEEGSPYSGQDANCGNTLLISLDELVKDGLLTKDELPKPV+ DHV
Sbjct: 121 SVWQVLPLVPPGRKANEEGSPYSGQDANCGNTLLISLDELVKDGLLTKDELPKPVNHDHV 180

Query: 181 NFSAVADIKDPLIAKAAERLIQSDGELKRQLEEFRRDPDISSWLEDAAYFAAIDNRLNYF 240
           NFSAVADIKDPLIAKAAERLIQSDGELK QLEEF RDPDISSWLEDAAYFAAIDNRLN F
Sbjct: 181 NFSAVADIKDPLIAKAAERLIQSDGELKCQLEEFCRDPDISSWLEDAAYFAAIDNRLNCF 240

Query: 241 SWYEWPEPLKNRHLSALEDVYQRERDFINIFIAQQFLFQRQWQRVRSYANMKGITIMGDM 300
           SWYEWPEPLKNRHLSALEDVYQRERDFINIFIAQQFLFQRQWQRVRSYANMKGITIMGDM
Sbjct: 241 SWYEWPEPLKNRHLSALEDVYQRERDFINIFIAQQFLFQRQWQRVRSYANMKGITIMGDM 300

Query: 301 PIYVGYQSADVWANKKQFLLNKKGFPVLVSGVPPDAFSETGQLWGS 319
           PIYVGYQSADVWANKKQFLLNKKGFPVLVSGVPPDAFSETGQLWGS
Sbjct: 301 PIYVGYQSADVWANKKQFLLNKKGFPVLVSGVPPDAFSETGQLWGS 346

[Close]

BLAST of Lag0008017 vs. ExPASy TrEMBL
Match: A0A6J1DIV2 (4-alpha-glucanotransferase OS=Momordica charantia OX=3673 GN=LOC111021480 PE=3 SV=1)

HSP 1 Score: 545.0 bits (1403), Expect = 2.0e-151
Identity = 275/346 (79.48%), Postives = 287/346 (82.95%), Query Frame = 0


Query: 1   MAISASLLPFPLTPKLRNPHCRRP--------------------ASQRHYYVPVHQEIPA 60
           MAIS SL PFPLTPKLRNP    P                    +S   + + VH    +
Sbjct: 1   MAISTSLFPFPLTPKLRNPPLSPPPLPLNASTATLSTRRFGPLSSSSSTHNLTVHAIFDS 60

Query: 61  LVI--------FFLYPQSESWHHFRYSVLLHPTSFRGPYGIGDLGDEAFRFIDWLHDAGF 120
           + +           +P++      R  VLLHPTSFRGPYGIGDLGDEAFRF+DWLH+AGF
Sbjct: 61  VGVGEDFPPHYGEWFPETNPDVRRRAGVLLHPTSFRGPYGIGDLGDEAFRFVDWLHEAGF 120

Query: 121 SVWQVLPLVPPGRKANEEGSPYSGQDANCGNTLLISLDELVKDGLLTKDELPKPVDPDHV 180
           SVWQVLPLVPPGRKANEEGSPYSGQDANCGNTLLISLDELVKDGLLTKDELPKPV+ DHV
Sbjct: 121 SVWQVLPLVPPGRKANEEGSPYSGQDANCGNTLLISLDELVKDGLLTKDELPKPVNHDHV 180

Query: 181 NFSAVADIKDPLIAKAAERLIQSDGELKRQLEEFRRDPDISSWLEDAAYFAAIDNRLNYF 240
           NFSAVADIKDPLIAKAAERLIQSDGELK QLEEF RDPDISSWLEDAAYFAAIDNRLN F
Sbjct: 181 NFSAVADIKDPLIAKAAERLIQSDGELKCQLEEFCRDPDISSWLEDAAYFAAIDNRLNCF 240

Query: 241 SWYEWPEPLKNRHLSALEDVYQRERDFINIFIAQQFLFQRQWQRVRSYANMKGITIMGDM 300
           SWYEWPEPLKNRHLSALEDVYQRERDFINIFIAQQFLFQRQWQRVRSYANMKGITIMGDM
Sbjct: 241 SWYEWPEPLKNRHLSALEDVYQRERDFINIFIAQQFLFQRQWQRVRSYANMKGITIMGDM 300

Query: 301 PIYVGYQSADVWANKKQFLLNKKGFPVLVSGVPPDAFSETGQLWGS 319
           PIYVGYQSADVWANKKQFLLNKKGFPVLVSGVPPDAFSETGQLWGS
Sbjct: 301 PIYVGYQSADVWANKKQFLLNKKGFPVLVSGVPPDAFSETGQLWGS 346

[Close]

BLAST of Lag0008017 vs. ExPASy TrEMBL
Match: A0A5D3CP83 (4-alpha-glucanotransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G002270 PE=3 SV=1)

HSP 1 Score: 533.1 bits (1372), Expect = 8.1e-148
Identity = 270/341 (79.18%), Postives = 280/341 (82.11%), Query Frame = 0


Query: 1   MAISASLLPFPLTPKLRNP---------HCRRPASQRHYYVPVHQEIPALVIFFLYPQSE 60
           MAIS SL PFPL PKLRNP               S R + +          IF      E
Sbjct: 1   MAISTSLFPFPLNPKLRNPPLPLFTTTTTTTTSLSTRRFRLFSSSSSKVSTIFDTVTVGE 60

Query: 61  SW-HHF-------------RYSVLLHPTSFRGPYGIGDLGDEAFRFIDWLHDAGFSVWQV 120
            +  H+             R  VLLHPTSFRGPYGIGDLGDEAFRF+DWLH+AGFSVWQV
Sbjct: 61  DFPTHYGEGFLESDPDIRRRAGVLLHPTSFRGPYGIGDLGDEAFRFVDWLHEAGFSVWQV 120

Query: 121 LPLVPPGRKANEEGSPYSGQDANCGNTLLISLDELVKDGLLTKDELPKPVDPDHVNFSAV 180
           LPLVPPGRKANEEGSPYSGQDANCGNTLLISLDELVKDGLLTKDELPKPVD DHV FSAV
Sbjct: 121 LPLVPPGRKANEEGSPYSGQDANCGNTLLISLDELVKDGLLTKDELPKPVDHDHVVFSAV 180

Query: 181 ADIKDPLIAKAAERLIQSDGELKRQLEEFRRDPDISSWLEDAAYFAAIDNRLNYFSWYEW 240
           ADIKDPLIAKAAERLIQSDG+LKRQLEEF RDPDISSWLEDAAYFAAIDN+LN FSWYEW
Sbjct: 181 ADIKDPLIAKAAERLIQSDGDLKRQLEEFCRDPDISSWLEDAAYFAAIDNKLNSFSWYEW 240

Query: 241 PEPLKNRHLSALEDVYQRERDFINIFIAQQFLFQRQWQRVRSYANMKGITIMGDMPIYVG 300
           PEPLKNRHLSAL +VYQ ERDFINIFIAQQFLFQRQWQRVRSYANMKGITIMGDMPIYVG
Sbjct: 241 PEPLKNRHLSALVEVYQTERDFINIFIAQQFLFQRQWQRVRSYANMKGITIMGDMPIYVG 300

Query: 301 YQSADVWANKKQFLLNKKGFPVLVSGVPPDAFSETGQLWGS 319
           YQSADVWANKKQFLLNKKGFP+LVSGVPPDAFSETGQLWGS
Sbjct: 301 YQSADVWANKKQFLLNKKGFPLLVSGVPPDAFSETGQLWGS 341

[Close]

BLAST of Lag0008017 vs. ExPASy TrEMBL
Match: A0A1S3C639 (4-alpha-glucanotransferase OS=Cucumis melo OX=3656 GN=LOC103497466 PE=3 SV=1)

HSP 1 Score: 533.1 bits (1372), Expect = 8.1e-148
Identity = 270/341 (79.18%), Postives = 280/341 (82.11%), Query Frame = 0


Query: 1   MAISASLLPFPLTPKLRNP---------HCRRPASQRHYYVPVHQEIPALVIFFLYPQSE 60
           MAIS SL PFPL PKLRNP               S R + +          IF      E
Sbjct: 1   MAISTSLFPFPLNPKLRNPPLPLFTTTTTTTTSLSTRRFRLFSSSSSKVSTIFDTVTVGE 60

Query: 61  SW-HHF-------------RYSVLLHPTSFRGPYGIGDLGDEAFRFIDWLHDAGFSVWQV 120
            +  H+             R  VLLHPTSFRGPYGIGDLGDEAFRF+DWLH+AGFSVWQV
Sbjct: 61  DFPTHYGEGFLESDPDIRRRAGVLLHPTSFRGPYGIGDLGDEAFRFVDWLHEAGFSVWQV 120

Query: 121 LPLVPPGRKANEEGSPYSGQDANCGNTLLISLDELVKDGLLTKDELPKPVDPDHVNFSAV 180
           LPLVPPGRKANEEGSPYSGQDANCGNTLLISLDELVKDGLLTKDELPKPVD DHV FSAV
Sbjct: 121 LPLVPPGRKANEEGSPYSGQDANCGNTLLISLDELVKDGLLTKDELPKPVDHDHVVFSAV 180

Query: 181 ADIKDPLIAKAAERLIQSDGELKRQLEEFRRDPDISSWLEDAAYFAAIDNRLNYFSWYEW 240
           ADIKDPLIAKAAERLIQSDG+LKRQLEEF RDPDISSWLEDAAYFAAIDN+LN FSWYEW
Sbjct: 181 ADIKDPLIAKAAERLIQSDGDLKRQLEEFCRDPDISSWLEDAAYFAAIDNKLNSFSWYEW 240

Query: 241 PEPLKNRHLSALEDVYQRERDFINIFIAQQFLFQRQWQRVRSYANMKGITIMGDMPIYVG 300
           PEPLKNRHLSAL +VYQ ERDFINIFIAQQFLFQRQWQRVRSYANMKGITIMGDMPIYVG
Sbjct: 241 PEPLKNRHLSALVEVYQTERDFINIFIAQQFLFQRQWQRVRSYANMKGITIMGDMPIYVG 300

Query: 301 YQSADVWANKKQFLLNKKGFPVLVSGVPPDAFSETGQLWGS 319
           YQSADVWANKKQFLLNKKGFP+LVSGVPPDAFSETGQLWGS
Sbjct: 301 YQSADVWANKKQFLLNKKGFPLLVSGVPPDAFSETGQLWGS 341

[Close]

BLAST of Lag0008017 vs. ExPASy TrEMBL
Match: A0A0A0LQ32 (4-alpha-glucanotransferase OS=Cucumis sativus OX=3659 GN=Csa_2G296010 PE=3 SV=1)

HSP 1 Score: 531.6 bits (1368), Expect = 2.3e-147
Identity = 265/337 (78.64%), Postives = 281/337 (83.38%), Query Frame = 0


Query: 1   MAISASLLPFPLTPKLRNP-----HCRRPASQRHYYVPVHQEIPALVIFFL--------- 60
           MAIS SL  FPL PKLRNP           S R +++ +        IF           
Sbjct: 1   MAISTSLFSFPLAPKLRNPSLPLFSATTSLSTRRFHLFLSSSSKVSTIFDTVTVGEDFPT 60

Query: 61  -----YPQSESWHHFRYSVLLHPTSFRGPYGIGDLGDEAFRFIDWLHDAGFSVWQVLPLV 120
                +P+S+     R  VLLHPTSFRGPYGIGDLGDEAFRF+DWLH+AGFSVWQVLPLV
Sbjct: 61  QYGEWFPESDPDIRRRAGVLLHPTSFRGPYGIGDLGDEAFRFVDWLHEAGFSVWQVLPLV 120

Query: 121 PPGRKANEEGSPYSGQDANCGNTLLISLDELVKDGLLTKDELPKPVDPDHVNFSAVADIK 180
           PPGRKANEEGSPYSGQDANCGNTLLISLDELVKDGLLTKDELP+PVD DHV FS VADIK
Sbjct: 121 PPGRKANEEGSPYSGQDANCGNTLLISLDELVKDGLLTKDELPEPVDHDHVVFSTVADIK 180

Query: 181 DPLIAKAAERLIQSDGELKRQLEEFRRDPDISSWLEDAAYFAAIDNRLNYFSWYEWPEPL 240
           DPLIAKAAERLIQSDG+LKRQLEEF +DP+ISSWLEDAAYFAAIDNRLN FSWYEWPEPL
Sbjct: 181 DPLIAKAAERLIQSDGDLKRQLEEFCKDPEISSWLEDAAYFAAIDNRLNSFSWYEWPEPL 240

Query: 241 KNRHLSALEDVYQRERDFINIFIAQQFLFQRQWQRVRSYANMKGITIMGDMPIYVGYQSA 300
           KNRHLSAL +V+Q ERDFINIFIAQQFLFQRQWQRVRSYANMKGITIMGDMPIYVGYQSA
Sbjct: 241 KNRHLSALVEVFQTERDFINIFIAQQFLFQRQWQRVRSYANMKGITIMGDMPIYVGYQSA 300

Query: 301 DVWANKKQFLLNKKGFPVLVSGVPPDAFSETGQLWGS 319
           DVWANKKQFLLNKKGFP+LVSGVPPDAFSETGQLWGS
Sbjct: 301 DVWANKKQFLLNKKGFPLLVSGVPPDAFSETGQLWGS 337

[Close]

BLAST of Lag0008017 vs. TAIR 10
Match: AT5G64860.1 (disproportionating enzyme )

HSP 1 Score: 427.6 bits (1098), Expect = 9.2e-120
Identity = 198/262 (75.57%), Postives = 224/262 (85.50%), Query Frame = 0


Query: 57  RYSVLLHPTSFRGPYGIGDLGDEAFRFIDWLHDAGFSVWQVLPLVPPGRKANEEGSPYSG 116
           R  VLLHPTSFRGP+GIGDLG+EAFRFIDWLH  G SVWQVLPLVPP    +E GSPY+G
Sbjct: 83  RAGVLLHPTSFRGPHGIGDLGEEAFRFIDWLHSTGCSVWQVLPLVPP----DEGGSPYAG 142

Query: 117 QDANCGNTLLISLDELVKDGLLTKDELPKPVDPDHVNFSAVADIKDPLIAKAAERLIQSD 176
           QDANCGNTLLISLDELVKDGLL KDELP+P+D D VN+     +K PLI KAA+RLI  +
Sbjct: 143 QDANCGNTLLISLDELVKDGLLIKDELPQPIDADSVNYQTANKLKSPLITKAAKRLIDGN 202

Query: 177 GELKRQLEEFRRDPDISSWLEDAAYFAAIDNRLNYFSWYEWPEPLKNRHLSALEDVYQRE 236
           GELK +L +FR DP IS WLEDAAYFAAIDN LN +SW+EWPEPLKNRHLSALE +Y+ +
Sbjct: 203 GELKSKLLDFRNDPSISCWLEDAAYFAAIDNTLNAYSWFEWPEPLKNRHLSALEAIYESQ 262

Query: 237 RDFINIFIAQQFLFQRQWQRVRSYANMKGITIMGDMPIYVGYQSADVWANKKQFLLNKKG 296
           ++FI++FIA+QFLFQRQWQ+VR YA  +G+ IMGDMPIYVGY SADVWANKK FLLNKKG
Sbjct: 263 KEFIDLFIAKQFLFQRQWQKVREYARRQGVDIMGDMPIYVGYHSADVWANKKHFLLNKKG 322

Query: 297 FPVLVSGVPPDAFSETGQLWGS 319
           FP+LVSGVPPD FSETGQLWGS
Sbjct: 323 FPLLVSGVPPDLFSETGQLWGS 340

[Close]

BLAST of Lag0008017 vs. TAIR 10
Match: AT2G40840.1 (disproportionating enzyme 2 )

HSP 1 Score: 65.9 bits (159), Expect = 7.0e-11
Identity = 62/257 (24.12%), Postives = 106/257 (41.25%), Query Frame = 0


Query: 72  GIGDLGDEAFRFIDWLHDAGFSVWQVLPLVPPG-RKANEEGSPYSGQDANCGNTLLI--- 131
           G+G+  D     +DW  D+G  + Q+LP+      K   +  PYS       + L +   
Sbjct: 287 GVGEFLDLKL-LVDWAVDSGLHLVQLLPVNDTSVHKMWWDSYPYSSLSVFALHPLYLRVQ 346

Query: 132 SLDELVKDGLLTKDELPK---PVDPDHVNFSAVADIKDPLIAKAAERLIQSDGELKRQ-L 191
           +L E + + +  K+E+ K    +D + V++ A  + K  +  K  +  I+ D  L     
Sbjct: 347 ALSERLPEDI--KEEIQKAKNQLDKNDVDYEATMETKLSIAKKIFD--IEKDQTLNSSTF 406

Query: 192 EEFRRDPDISSWLEDAAYFAAIDNRLNYFSWYEWPEPLKNRHLSALEDVYQRERDFINIF 251
           ++F  + +   WL+  A F  + +        +W     +     LE +  ++    N  
Sbjct: 407 QKFFSENE--GWLKPYAAFCFLRDFFETSDHSQW-GTFSDYTDDKLEKLISKDNLHYNTI 466

Query: 252 IAQ---QFLFQRQWQRVRSYANMKGITIMGDMPIYVGYQSADVWANKKQFLLNKKGFPVL 311
                 Q+    Q      YA  KG+ + GD+PI V   S D W  +  F +N       
Sbjct: 467 CFHYYIQYHLHVQLSAAAEYARKKGVVLKGDLPIGVDRNSVDTWVYRNLFRMNTS----- 526

Query: 312 VSGVPPDAFSETGQLWG 318
            +G PPD F + GQ WG
Sbjct: 527 -TGAPPDYFDKNGQNWG 529

The following BLAST results are available for this feature:

BLAST of Lag0008017 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022154165.1	4.2e-151	79.48	4-alpha-glucanotransferase, chloroplastic/amyloplastic isoform X2 [Momordica cha...	[more]
XP_022154164.1	4.2e-151	79.48	4-alpha-glucanotransferase, chloroplastic/amyloplastic isoform X1 [Momordica cha...	[more]
XP_038901901.1	6.1e-150	80.99	4-alpha-glucanotransferase, chloroplastic/amyloplastic isoform X5 [Benincasa his...	[more]
XP_038901898.1	6.1e-150	80.99	4-alpha-glucanotransferase, chloroplastic/amyloplastic isoform X2 [Benincasa his...	[more]
XP_038901900.1	1.5e-148	80.76	4-alpha-glucanotransferase, chloroplastic/amyloplastic isoform X4 [Benincasa his...	[more]

BLAST of Lag0008017 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q06801	3.2e-125	77.86	4-alpha-glucanotransferase, chloroplastic/amyloplastic OS=Solanum tuberosum OX=4...	[more]
Q9LV91	1.3e-118	75.57	4-alpha-glucanotransferase DPE1, chloroplastic/amyloplastic OS=Arabidopsis thali...	[more]
Q8LI30	4.6e-116	70.70	4-alpha-glucanotransferase DPE1, chloroplastic/amyloplastic OS=Oryza sativa subs...	[more]
O66937	3.4e-50	41.70	4-alpha-glucanotransferase OS=Aquifex aeolicus (strain VF5) OX=224324 GN=malQ PE...	[more]
P72785	7.2e-45	41.29	4-alpha-glucanotransferase OS=Synechocystis sp. (strain PCC 6803 / Kazusa) OX=11...	[more]

BLAST of Lag0008017 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1DL91	2.0e-151	79.48	4-alpha-glucanotransferase OS=Momordica charantia OX=3673 GN=LOC111021480 PE=3 S...	[more]
A0A6J1DIV2	2.0e-151	79.48	4-alpha-glucanotransferase OS=Momordica charantia OX=3673 GN=LOC111021480 PE=3 S...	[more]
A0A5D3CP83	8.1e-148	79.18	4-alpha-glucanotransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A1S3C639	8.1e-148	79.18	4-alpha-glucanotransferase OS=Cucumis melo OX=3656 GN=LOC103497466 PE=3 SV=1	[more]
A0A0A0LQ32	2.3e-147	78.64	4-alpha-glucanotransferase OS=Cucumis sativus OX=3659 GN=Csa_2G296010 PE=3 SV=1	[more]

BLAST of Lag0008017 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G64860.1	9.2e-120	75.57	disproportionating enzyme	[more]
AT2G40840.1	7.0e-11	24.12	disproportionating enzyme 2	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

Zoom :

x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003385	Glycoside hydrolase, family 77	PFAM	PF02446	Glyco_hydro_77	coord: 62..318 e-value: 6.2E-69 score: 233.1
IPR003385	Glycoside hydrolase, family 77	PANTHER	PTHR32438	4-ALPHA-GLUCANOTRANSFERASE DPE1, CHLOROPLASTIC/AMYLOPLASTIC	coord: 55..318
None	No IPR available	GENE3D	3.20.20.80	Glycosidases	coord: 57..322 e-value: 4.3E-86 score: 291.5
IPR017853	Glycoside hydrolase superfamily	SUPERFAMILY	51445	(Trans)glycosidases	coord: 53..319

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0008017.1	Lag0008017.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006006	glucose metabolic process
biological_process	GO:0005977	glycogen metabolic process
biological_process	GO:0000025	maltose catabolic process
biological_process	GO:0005983	starch catabolic process
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004134	4-alpha-glucanotransferase activity
molecular_function	GO:0102500	beta-maltose 4-alpha-glucanotransferase activity