Lag0007992 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0007992 vs. NCBI nr
Match: XP_038902853.1 (probable pectinesterase/pectinesterase inhibitor 20 [Benincasa hispida])

HSP 1 Score: 912.1 bits (2356), Expect = 2.3e-261
Identity = 459/562 (81.67%), Postives = 496/562 (88.26%), Query Frame = 0

Query: 1   MASSNSIPPLTILTLLLFLFSSSSSAVPVPGPVPTEEICRSTTSPFFCKSFLPNSPATVH 60
           M SSNSI  LT LT  LFL  SS SAV     VPT EICRSTT P FC +FLPNSPA+VH
Sbjct: 1   MVSSNSISLLTFLT-FLFLLPSSFSAV-----VPTTEICRSTTFPSFCNAFLPNSPASVH 60

Query: 61  SHCRYTLRHALVHARTFLALVDTHITLFPFLSALQDCRSLAAANLDFISNSFHTVNTTSQ 120
           SHCR+TL HAL HA+TFLALVD H++LFPF SAL DCRSLA ANLD+I+ +F  VN+TS 
Sbjct: 61  SHCRFTLHHALTHAQTFLALVDAHLSLFPFPSALHDCRSLAEANLDYITQTFSIVNSTSV 120

Query: 121 ALPFYQADEMLSLLSAVITNEDTCYEGLVGLGVGLGLVDKVLKAISFDRKLYSLYLSLFK 180
           ALP+YQA EM+SL+SA+ITNE+TCYEGL GLG  +GLVDKVL+AISFDRKLYSLYLSLFK
Sbjct: 121 ALPYYQAHEMMSLMSAMITNENTCYEGLAGLGSAIGLVDKVLEAISFDRKLYSLYLSLFK 180

Query: 181 LGWVSDDVKAPELPKTNRFGLGNGALKLKMSPKDRAYYERLVHRKKP-AARRLLQTNNYE 240
           +GWVS ++KAP LPK N FG+G G LKLKMSPKDRAYYERLVH KKP AARRLLQT NYE
Sbjct: 181 MGWVSKNMKAPILPKMNHFGVGKGQLKLKMSPKDRAYYERLVHSKKPAAARRLLQT-NYE 240

Query: 241 NDGIVINGIVGVDQNGTYDFTTINAAIAAAPNNSVAAKGYYLIFVTAGIYNETVLVPKNK 300
           NDGI++NG+V VDQNGTYDFT I+AAIAAAP+ +   KGY+LIFVTAGIYNETVLVPKNK
Sbjct: 241 NDGILVNGVVAVDQNGTYDFTNISAAIAAAPDKTTVTKGYFLIFVTAGIYNETVLVPKNK 300

Query: 301 KYILMIGEGNNQTIITGNKNVVDGSTTFNSATVAVEGAGFFGVNLTITNTAGAAKHQAVA 360
           +Y+L+IGEGNNQTII GNKNVVDGSTTFNSATVAVEG GFFGVNLTI+NTAG AKHQAVA
Sbjct: 301 RYVLLIGEGNNQTIINGNKNVVDGSTTFNSATVAVEGTGFFGVNLTISNTAGPAKHQAVA 360

Query: 361 LRVSADMTTFYTCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQTCNIYARL 420
           LRVSAD  T Y+CIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVV Q CNIYARL
Sbjct: 361 LRVSADNATLYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARL 420

Query: 421 PLSGQFNALTAQGRTDPNQNTGTSIHNCTLKPTAELAASPTTKSYLGRPWKQYSRTVYMQ 480
           PLSGQFNALTAQGRTDPNQNTGTSIHNCT+K T ELAA+P TKSYLGRPWK+YSRTVYMQ
Sbjct: 421 PLSGQFNALTAQGRTDPNQNTGTSIHNCTIKATPELAANPATKSYLGRPWKEYSRTVYMQ 480

Query: 481 SFIDAFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSHRVQWAVGAINATVANNFTVS 540
           SFIDAFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTS R  W VG INATVAN+FTVS
Sbjct: 481 SFIDAFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSRRAPWVVGVINATVANSFTVS 540

Query: 541 NFLAGDQWLPPTTVPYTGSLIS 562
            FLAGDQWLPPT VPYTG LIS
Sbjct: 541 QFLAGDQWLPPTAVPYTGGLIS 555

BLAST of Lag0007992 vs. NCBI nr
Match: XP_022928019.1 (pectinesterase-like [Cucurbita moschata])

HSP 1 Score: 903.7 bits (2334), Expect = 8.1e-259
Identity = 453/561 (80.75%), Postives = 494/561 (88.06%), Query Frame = 0

Query: 1   MASSNSIPPLTILTLLLFLFSSSSSAVPVPGPVPTEEICRSTTSPFFCKSFLPNSPATVH 60
           MASS++   L +   LLFLF+SSS+       VPT E+C+S TSP FCKSFLP+SPA+VH
Sbjct: 1   MASSSNPISLLLFFSLLFLFTSSSA-------VPTVEVCQSATSPSFCKSFLPSSPASVH 60

Query: 61  SHCRYTLRHALVHARTFLALVDTHITLFPFLSALQDCRSLAAANLDFISNSFHTVNTTSQ 120
           SHCRYTL HALV ARTF+ LVD  +TLFP LSAL DCRSLA ANLDFIS++  TVNTT+ 
Sbjct: 61  SHCRYTLHHALVQARTFVTLVDLQLTLFPSLSALHDCRSLAVANLDFISSTVDTVNTTTN 120

Query: 121 ALPFYQADEMLSLLSAVITNEDTCYEGLVGLGVGLGLVDKVLKAISFDRKLYSLYLSLFK 180
           ALPFYQADEM+SLLSAVITN DTC++GL G G+GLG VDKV+KAISFDR+LYSLYLSLFK
Sbjct: 121 ALPFYQADEMISLLSAVITNIDTCHDGLAGSGLGLGFVDKVVKAISFDRQLYSLYLSLFK 180

Query: 181 LGWVSDDVKAPELPKTNRFGLGNGALKLKMSPKDRAYYERLVHRKKPAARRLLQTNNYEN 240
           +GWVS ++KAP+LPKTN FG G G LKLKMSPKDRAYYERLVH KKPAARRLLQT NY +
Sbjct: 181 MGWVSKNMKAPKLPKTNHFGFGKGPLKLKMSPKDRAYYERLVHGKKPAARRLLQT-NYAD 240

Query: 241 DGIVINGIVGVDQNGTYDFTTINAAIAAAPNNSVAAKGYYLIFVTAGIYNETVLVPKNKK 300
           DGI++NG+V VDQNG YDFTTIN AIAAAPN S AA GY+LIFVTAGIY E+V+VPK+KK
Sbjct: 241 DGILVNGVVAVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVVVPKSKK 300

Query: 301 YILMIGEGNNQTIITGNKNVVDGSTTFNSATVAVEGAGFFGVNLTITNTAGAAKHQAVAL 360
           Y+LMIGEGNN+TIITGNKNVVDGSTTFNSATV VEG GFFG NLTITNTAGA KHQAVAL
Sbjct: 301 YVLMIGEGNNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIKHQAVAL 360

Query: 361 RVSADMTTFYTCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQTCNIYARLP 420
           RVSADMTTFY+CIFEGYQDTLY HSLRQFYRECDIYGTVDFIFGNAAVV Q CNIYARLP
Sbjct: 361 RVSADMTTFYSCIFEGYQDTLYAHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLP 420

Query: 421 LSGQFNALTAQGRTDPNQNTGTSIHNCTLKPTAELAASPTTKSYLGRPWKQYSRTVYMQS 480
           LSGQFNALTAQGRTD NQNTG  IH CT+KPT +LAASPTTKSYLGRPWK++SRTVYMQS
Sbjct: 421 LSGQFNALTAQGRTDQNQNTGIFIHKCTIKPTPDLAASPTTKSYLGRPWKEFSRTVYMQS 480

Query: 481 FIDAFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSHRVQWAVGAINATVANNFTVSN 540
           FIDAFIDPAGWKEWDGTLNLNTSYYAEF+NSGPGSDTS R QWAVG INATVAN FTVS 
Sbjct: 481 FIDAFIDPAGWKEWDGTLNLNTSYYAEFDNSGPGSDTSQRAQWAVGVINATVANIFTVSE 540

Query: 541 FLAGDQWLPPTTVPYTGSLIS 562
            LAGDQWLPPT VPY+G LIS
Sbjct: 541 ILAGDQWLPPTAVPYSGGLIS 553

BLAST of Lag0007992 vs. NCBI nr
Match: XP_023007148.1 (probable pectinesterase/pectinesterase inhibitor 20 [Cucurbita maxima])

HSP 1 Score: 902.5 bits (2331), Expect = 1.8e-258
Identity = 452/565 (80.00%), Postives = 495/565 (87.61%), Query Frame = 0

Query: 1   MASSNSIPPLTILTLLLFLFSSSSSAVPVPGPVPTEEICRSTTSPFFCKSFLPNSPATVH 60
           M SS++IP LT LT LL+LFS SSSA  +  PVP  +IC STTSP FCK+FLPNS ATVH
Sbjct: 1   MGSSSAIPLLTFLT-LLYLFSFSSSA--IHSPVPAADICHSTTSPSFCKAFLPNSLATVH 60

Query: 61  SHCRYTLRHALVHARTFLALVDTHITLFPFLSALQDCRSLAAANLDFISNSFHTVN---- 120
           SHCR+TLRHAL HARTFLALVDTH+TLFP L+AL DCRSLAAANLDF+SN+F TVN    
Sbjct: 61  SHCRFTLRHALAHARTFLALVDTHLTLFPSLAALHDCRSLAAANLDFLSNTFPTVNTTTT 120

Query: 121 TTSQALPFYQADEMLSLLSAVITNEDTCYEGLVGLGVGLGLVDKVLKAISFDRKLYSLYL 180
           TT+ ALPFY+A+EM+SLLSAVITNE+TCYEGL+  G   GLVDKVLKAISFD KLYSLYL
Sbjct: 121 TTTNALPFYKAEEMVSLLSAVITNENTCYEGLMSSGSASGLVDKVLKAISFDTKLYSLYL 180

Query: 181 SLFKLGWVSDDVKAPELPKTNRFGLGNGALKLKMSPKDRAYYERLVHRKKPAARRLLQTN 240
           SLFK+GWVS+DVKAP +PK   FG G G LKL MSPK+RAYYERL+HR KP ARRLLQTN
Sbjct: 181 SLFKMGWVSNDVKAPHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRSKPPARRLLQTN 240

Query: 241 NYENDGIVINGIVGVDQNGTYDFTTINAAIAAAPNNSVAAKGYYLIFVTAGIYNETVLVP 300
           + E+DGI++NGIV VDQNGTYDFTTINAAI AAPNNSVAA GY+LI VTAG+YNE+V+VP
Sbjct: 241 SNEDDGILVNGIVAVDQNGTYDFTTINAAIQAAPNNSVAADGYFLIIVTAGVYNESVMVP 300

Query: 301 KNKKYILMIGEGNNQTIITGNKNVVDGSTTFNSATVAVEGAGFFGVNLTITNTAGAAKHQ 360
           K+KKY+LMIGEGNNQTIITGNKNVVDGSTTFNS T  VEG GFFGVNLTITNTAG  K Q
Sbjct: 301 KHKKYVLMIGEGNNQTIITGNKNVVDGSTTFNSGTFIVEGTGFFGVNLTITNTAGPNKFQ 360

Query: 361 AVALRVSADMTTFYTCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQTCNIY 420
           AVALRVSADMTTFY+CIFEGYQDTLY HSLRQFYRECDIYGT+DFI G+AAVVFQ CNIY
Sbjct: 361 AVALRVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAAVVFQNCNIY 420

Query: 421 ARLPLSGQFNALTAQGRTDPNQNTGTSIHNCTLKPTAELAASPTTKSYLGRPWKQYSRTV 480
           ARLP+ GQ+  +TAQGRTD NQNTG SIHNCT+KPTA+LAASPT KSY GRPWK+Y+RTV
Sbjct: 421 ARLPMKGQYIVITAQGRTDRNQNTGISIHNCTIKPTAQLAASPTIKSYFGRPWKEYARTV 480

Query: 481 YMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSHRVQWAVGAINATVANNF 540
           YMQSFIDAFIDP GW  WDGTLNLNTSYYAEFNNSGPGSDTS R +WAVG INATVANNF
Sbjct: 481 YMQSFIDAFIDPEGWNVWDGTLNLNTSYYAEFNNSGPGSDTSRRAKWAVGLINATVANNF 540

Query: 541 TVSNFLAGDQWLPPTTVPYTGSLIS 562
           TVS FL GDQWLPPTTVPYTG LIS
Sbjct: 541 TVSPFLGGDQWLPPTTVPYTGGLIS 562

BLAST of Lag0007992 vs. NCBI nr
Match: KAG6571391.1 (putative pectinesterase/pectinesterase inhibitor 41, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 901.4 bits (2328), Expect = 4.0e-258
Identity = 455/563 (80.82%), Postives = 495/563 (87.92%), Query Frame = 0

Query: 1   MASSNSIPPLTILTLLLFLFSSSSSAVPVPGPVPTEEICRSTTSPFFCKSFLPNSPATVH 60
           MASS++   L +   LLFLF+SSS+       VPT E+C+S TSP FCKSFLP+SPA+VH
Sbjct: 1   MASSSNPISLLLFFSLLFLFTSSSA-------VPTVEVCQSATSPSFCKSFLPSSPASVH 60

Query: 61  SHCRYTLRHALVHARTFLALVDTHITLFPFLSALQDCRSLAAANLDFISNSFHTVNTTSQ 120
           SHCRYTL HALV ARTF+ LVD  +TLFP LSAL DCRSLA ANLDFIS++  TVNTT+ 
Sbjct: 61  SHCRYTLHHALVQARTFVTLVDLQLTLFPSLSALHDCRSLAEANLDFISSTVDTVNTTTN 120

Query: 121 ALPFYQADEMLSLLSAVITNEDTCYEGLV--GLGVGLGLVDKVLKAISFDRKLYSLYLSL 180
           ALPFYQADEM+SLLSAVITN DTC++GL   G G GLGLVDKV+KAISFDR+LYSLYLSL
Sbjct: 121 ALPFYQADEMISLLSAVITNVDTCHDGLAGSGSGSGLGLVDKVVKAISFDRQLYSLYLSL 180

Query: 181 FKLGWVSDDVKAPELPKTNRFGLGNGALKLKMSPKDRAYYERLVHRKKPAARRLLQTNNY 240
           FK+GWVS ++KAP+LPKTN FG GNG LKLKMSPKDRAYYERLVH KKPAARRLLQT NY
Sbjct: 181 FKMGWVSKNMKAPKLPKTNHFGFGNGPLKLKMSPKDRAYYERLVHGKKPAARRLLQT-NY 240

Query: 241 ENDGIVINGIVGVDQNGTYDFTTINAAIAAAPNNSVAAKGYYLIFVTAGIYNETVLVPKN 300
            +DGI++NG+V VDQNG YDFTTIN AIAAAPN S AA GY+LIFVTAGIY E+V+VPK+
Sbjct: 241 ADDGILVNGVVAVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVVVPKS 300

Query: 301 KKYILMIGEGNNQTIITGNKNVVDGSTTFNSATVAVEGAGFFGVNLTITNTAGAAKHQAV 360
           KKY+LMIGEGNN+TIITGNKNVVDGSTTFNSATV VEG GFFG NLTITNTAGA KHQAV
Sbjct: 301 KKYVLMIGEGNNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIKHQAV 360

Query: 361 ALRVSADMTTFYTCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQTCNIYAR 420
           ALRVSADMTTFY+CIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVV Q CNIYAR
Sbjct: 361 ALRVSADMTTFYSCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYAR 420

Query: 421 LPLSGQFNALTAQGRTDPNQNTGTSIHNCTLKPTAELAASPTTKSYLGRPWKQYSRTVYM 480
           LPLSGQFNALTAQGRTD NQNTG  IH CT+KPT +LAASP TKSYLGRPWK++SRTVYM
Sbjct: 421 LPLSGQFNALTAQGRTDQNQNTGIFIHKCTIKPTPDLAASPATKSYLGRPWKEFSRTVYM 480

Query: 481 QSFIDAFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSHRVQWAVGAINATVANNFTV 540
           QSFIDAFIDPAGWKEWDGTLNLNTSYYAEF+NSGPGSDTS R QWAVG INATVAN FTV
Sbjct: 481 QSFIDAFIDPAGWKEWDGTLNLNTSYYAEFDNSGPGSDTSRRAQWAVGVINATVANIFTV 540

Query: 541 SNFLAGDQWLPPTTVPYTGSLIS 562
           S  LAGDQWLPPT VPY+G LIS
Sbjct: 541 SEILAGDQWLPPTAVPYSGGLIS 555

BLAST of Lag0007992 vs. NCBI nr
Match: XP_008457895.1 (PREDICTED: pectinesterase-like [Cucumis melo] >KAA0045890.1 pectinesterase-like [Cucumis melo var. makuwa] >TYK13697.1 pectinesterase-like [Cucumis melo var. makuwa])

HSP 1 Score: 900.2 bits (2325), Expect = 9.0e-258
Identity = 455/563 (80.82%), Postives = 489/563 (86.86%), Query Frame = 0

Query: 1   MASSNSIPPLTILTLLLFLFSSSSSAVPVPGPVPTEEICRSTTSPFFCKSFLPNSPATVH 60
           M SSN I  LT LTLL FL  SSS+       VPT EIC+STTSP FC  FLPNSPA+VH
Sbjct: 1   MVSSNPISVLTFLTLLFFLSPSSSA-------VPTAEICQSTTSPPFCNFFLPNSPASVH 60

Query: 61  SHCRYTLRHALVHARTFLALVDTHITLFPFLSALQDCRSLAAANLDFISNSFHTVN-TTS 120
           SHCR+TL HALVHARTFL+LV+ H+ LFP LS L DCRSLA ANLDF+S +F  VN TT+
Sbjct: 61  SHCRFTLHHALVHARTFLSLVNAHLNLFPSLSPLHDCRSLAEANLDFLSQTFSIVNSTTT 120

Query: 121 QALPFYQADEMLSLLSAVITNEDTCYEGLVGLGVGLGLVDKVLKAISFDRKLYSLYLSLF 180
             LP+Y A E L+L+SAVITNEDTCYEGL GL   +GLVDKVL+AISFD+KLYSLYLSLF
Sbjct: 121 TLLPYYDAHEKLTLMSAVITNEDTCYEGLAGLDSAVGLVDKVLEAISFDKKLYSLYLSLF 180

Query: 181 KLGWVSDDVKAPELPKTNRFGLGNGALKLKMSPKDRAYYERLVHRKK-PAARRLLQTNNY 240
           K+GWVS D+KAP LPK   FG+G G LKLKMSPKDRAYYERLVHR K P ARRLLQT NY
Sbjct: 181 KMGWVSKDLKAPTLPKMKHFGVGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQT-NY 240

Query: 241 ENDGIVINGIVGVDQNGTYDFTTINAAIAAAPNNSVAAKGYYLIFVTAGIYNETVLVPKN 300
           ++DGIVINGIVGVDQNGTYDFT I AAIAAAPN +  AKGY+LIFV AGIYNETVL+PK 
Sbjct: 241 QDDGIVINGIVGVDQNGTYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLIPKE 300

Query: 301 KKYILMIGEGNNQTIITGNKNVVDGSTTFNSATVAVEGAGFFGVNLTITNTAGAAKHQAV 360
           K+Y+L+IGEGNNQTIITGNKNVVDGSTTFNSATVAVEG GF GVNLTI+NTAGAAKHQAV
Sbjct: 301 KRYVLLIGEGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTISNTAGAAKHQAV 360

Query: 361 ALRVSADMTTFYTCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQTCNIYAR 420
           ALRVSAD  T Y CIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVV Q CNIYAR
Sbjct: 361 ALRVSADNATLYNCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYAR 420

Query: 421 LPLSGQFNALTAQGRTDPNQNTGTSIHNCTLKPTAELAASPTTKSYLGRPWKQYSRTVYM 480
           LPLSGQFNA+TAQGRTDPNQNTGTSIHNCT+K T ELAASP TKSYLGRPWKQYSRTVYM
Sbjct: 421 LPLSGQFNAVTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYM 480

Query: 481 QSFIDAFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSHRVQWAVGAINATVANNFTV 540
           QSFID+FIDPAGWKEWDGT+NLNTSYYAEFNNSGPGSDTS RV WAVG INATVAN+FTV
Sbjct: 481 QSFIDSFIDPAGWKEWDGTMNLNTSYYAEFNNSGPGSDTSQRVSWAVGVINATVANSFTV 540

Query: 541 SNFLAGDQWLPPTTVPYTGSLIS 562
           S FLAGD+WLPPT VPYTG LIS
Sbjct: 541 SQFLAGDRWLPPTDVPYTGGLIS 555

BLAST of Lag0007992 vs. ExPASy Swiss-Prot
Match: O22256 (Probable pectinesterase/pectinesterase inhibitor 20 OS=Arabidopsis thaliana OX=3702 GN=PME20 PE=2 SV=2)

HSP 1 Score: 534.6 bits (1376), Expect = 1.3e-150
Identity = 288/570 (50.53%), Postives = 378/570 (66.32%), Query Frame = 0

Query: 16  LLFLFSSS----------SSAVPVPGPVPT-EEICRSTTSPFFCKSFLPNSPATVHSHCR 75
           L+FLF+ +          S+ +P  G   +   ICR    P +C+S LPN P  ++S+ R
Sbjct: 5   LMFLFTLACLSSLPSPFISAQIPAIGNATSPSNICRFAPDPSYCRSVLPNQPGDIYSYGR 64

Query: 76  YTLRHALVHARTFLALVDTH------ITLFPFLSALQDCRSLAAANLDFISNSFHTVNTT 135
            +LR +L  AR F++++D        +     + AL+DC+ LA+  +D++ +S  T ++T
Sbjct: 65  LSLRRSLSRARRFISMIDAELDRKGKVAAKSTVGALEDCKFLASLTMDYLLSSSQTADST 124

Query: 136 SQALPFYQADEMLSLLSAVITNEDTCYEGLVGLGVGLGLVDKVLKAISFDRKLYSLYLSL 195
            + L   +A+++ + LSA ITNE TC EGL       GL   +      D KLY + L+L
Sbjct: 125 -KTLSLSRAEDVHTFLSAAITNEQTCLEGLKSTASENGLSGDLFN----DTKLYGVSLAL 184

Query: 196 FKLGWV-----SDDVKAPELPKTNRFGLGNGALKLKMSPKDRAYYERLVHRKKPAARRLL 255
           F  GWV     S  +  P+      FG  NG L LKM+ + RA Y  +  RK      LL
Sbjct: 185 FSKGWVPRRQRSRPIWQPQARFKKFFGFRNGKLPLKMTERARAVYNTVTRRK------LL 244

Query: 256 QTNNYENDGIVINGIVGVDQNGTYDFTTINAAIAAAPNNSVAAKGYYLIFVTAGIYNETV 315
           Q+   + D + ++ IV V QNGT +FTTINAAIAAAPN +  + GY+LI+VTAG+Y E V
Sbjct: 245 QS---DADAVQVSDIVTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYV 304

Query: 316 LVPKNKKYILMIGEGNNQTIITGNKNVVDGSTTFNSATVAVEGAGFFGVNLTITNTAGAA 375
            VPKNK+Y++MIG+G NQT+ITGN++VVDG TTFNSAT  + G  F GVN+TI NTAG  
Sbjct: 305 EVPKNKRYVMMIGDGINQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPT 364

Query: 376 KHQAVALRVSADMTTFYTCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQTC 435
           K QAVALR   D++ FY+C FE YQDTLYTHSLRQFYRECD+YGTVDFIFGNAAVV Q C
Sbjct: 365 KGQAVALRSGGDLSVFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNC 424

Query: 436 NIYARLPLSGQFNALTAQGRTDPNQNTGTSIHNCTLKPTAELAASP-TTKSYLGRPWKQY 495
           N+Y R P  GQ N +TAQGRTDPNQNTGT+IH CT++P  +LA S  T K+YLGRPWK+Y
Sbjct: 425 NLYPRQPRKGQSNEVTAQGRTDPNQNTGTAIHGCTIRPADDLATSNYTVKTYLGRPWKEY 484

Query: 496 SRTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSHRVQW-AVGAINAT 555
           SRTV MQ++ID F++P+GW  W G   L+T YYAE+NN+GPGSDT++RV W     INAT
Sbjct: 485 SRTVVMQTYIDGFLEPSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINAT 544

Query: 556 VANNFTVSNFLAGDQWLPPTTVPYTGSLIS 562
            A+NFTV+NFL G+ W+  T VP+ G LI+
Sbjct: 545 DASNFTVTNFLVGEGWIGQTGVPFVGGLIA 560

BLAST of Lag0007992 vs. ExPASy Swiss-Prot
Match: Q9SRX4 (Probable pectinesterase/pectinesterase inhibitor 7 OS=Arabidopsis thaliana OX=3702 GN=PME7 PE=2 SV=1)

HSP 1 Score: 523.5 bits (1347), Expect = 3.0e-147
Identity = 297/591 (50.25%), Postives = 380/591 (64.30%), Query Frame = 0

Query: 8   PPLTILTLLLFLFSSSSSAVPVPGPVPTEEICRSTTSPFFCKSFLPNSPATVHSHCRYTL 67
           P   ++TL  FL S  +S+  +     +  IC++T  P +CKS  P+S   V  +  +++
Sbjct: 4   PIFILITLSFFLQSVLASSQTLSN---SSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSI 63

Query: 68  RHALVHARTFLALVDTHITLFPFLS------ALQDCRSLAAANLDFISNSFHTVNTTS-- 127
           R +L  +R F+  VD +I     LS      ALQDCR LA   +D++  SF TVN TS  
Sbjct: 64  RKSLSQSRKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAK 123

Query: 128 ---QALPFYQADEMLSLLSAVITNEDTCYEGL-----------VGLGVGLGLVDKVLKAI 187
              + L F +AD++ +LLSA +TNE TC EGL           V  GV L LV+      
Sbjct: 124 TSFKPLSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVN------ 183

Query: 188 SFDRKLYSLYLSLFKLGWVSDDVK----------APELPKTNRFGL-GNGALKLKMSPKD 247
             D KL  + L+LF  GWV    K          +     T  F L  NGAL LKM+ K 
Sbjct: 184 --DTKLLGVSLALFTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKT 243

Query: 248 RAYYERLVHRKKPAARRLLQTNNYENDG--IVINGIVGVDQNGTYDFTTINAAIAAAPNN 307
           +A YE L  RK         +N   +DG  ++I+ IV V Q+GT +FT I AA+AAAPNN
Sbjct: 244 KAVYESLSRRKLADG----DSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNN 303

Query: 308 SVAAKGYYLIFVTAGIYNETVLVPKNKKYILMIGEGNNQTIITGNKNVVDGSTTFNSATV 367
           +  + G++LI+VTAGIY E + + KNK+Y++MIG+G NQT++TGN++VVDG TTFNSAT 
Sbjct: 304 TDGSAGFFLIYVTAGIYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATF 363

Query: 368 AVEGAGFFGVNLTITNTAGAAKHQAVALRVSADMTTFYTCIFEGYQDTLYTHSLRQFYRE 427
           AV    F  VN+T  NTAG  KHQAVALR  AD + FY+C FE YQDTLYTHSLRQFYRE
Sbjct: 364 AVTAPNFVAVNITFRNTAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRE 423

Query: 428 CDIYGTVDFIFGNAAVVFQTCNIYARLPLSGQFNALTAQGRTDPNQNTGTSIHNCTLKPT 487
           CD+YGTVDFIFGNAAVVFQ CN+Y R P+  QFNA+TAQGR+DPNQNTGTSI NCT+KP 
Sbjct: 424 CDVYGTVDFIFGNAAVVFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPA 483

Query: 488 AELAASP-TTKSYLGRPWKQYSRTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFNNS 547
            +L +S  T K+YLGRPWK+YSRTVYMQS+ID F++P GW+EW+G   L+T YYAE+NN+
Sbjct: 484 DDLVSSNYTVKTYLGRPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNT 543

Query: 548 GPGSDTSHRVQW-AVGAINATVANNFTVSNFLAGDQWLPPTTVPYTGSLIS 562
           GPGS+T++RV W     IN+T A NFTV+       W+  T VPYT  LIS
Sbjct: 544 GPGSNTTNRVTWPGYHVINSTDAANFTVTGLFIEADWIWKTGVPYTSGLIS 579

BLAST of Lag0007992 vs. ExPASy Swiss-Prot
Match: Q8RXK7 (Probable pectinesterase/pectinesterase inhibitor 41 OS=Arabidopsis thaliana OX=3702 GN=PME41 PE=2 SV=2)

HSP 1 Score: 522.7 bits (1345), Expect = 5.1e-147
Identity = 291/583 (49.91%), Postives = 381/583 (65.35%), Query Frame = 0

Query: 10  LTILTLLLFLFSSSSSAVPVPGPVPTEEICRSTTSPFFCKSFLP-NSPATVHSHCRYTLR 69
           +T+   L  LF +S + +P      +  IC++T  P FCKS  P  S   V  + R++LR
Sbjct: 9   VTLFLSLQTLFIASQTLLPSNS---SSTICKTTPDPKFCKSVFPQTSQGDVREYGRFSLR 68

Query: 70  HALVHARTFLALVDTHITLFPFL------SALQDCRSLAAANLDFISNSFHTVN--TTSQ 129
            +L  +R F   +D ++     L       ALQDCR LA+   D++  SF TVN  T+S+
Sbjct: 69  KSLTQSRKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSK 128

Query: 130 ALPFYQADEMLSLLSAVITNEDTCYEGL---------VGLGVGLGLVDKVLKAISFDRKL 189
            L F +ADE+ +LLSA +TNE TC +G+         +  GV L L++        D KL
Sbjct: 129 TLSFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLIN--------DTKL 188

Query: 190 YSLYLSLFKLGWVSDDVKAPEL-----PK-----TNRF-GLGNGALKLKMSPKDRAYYER 249
           +S+ L+LF  GWV    K         PK     T  F    NGAL LKM+   RA YE 
Sbjct: 189 FSVSLALFTKGWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYES 248

Query: 250 LVHRKKPAARRLLQTNNYENDGIVINGIVGVDQNGTYDFTTINAAIAAAPNNSVAAKGYY 309
           L       +RR L  ++ + + ++++ IV V+QNGT +FTTI  A+ +APN +    GY+
Sbjct: 249 L-------SRRKLADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYF 308

Query: 310 LIFVTAGIYNETVLVPKNKKYILMIGEGNNQTIITGNKNVVDGSTTFNSATVAVEGAGFF 369
           +I+VT+G+Y E V++ KNK+Y++MIG+G N+T++TGN+NVVDG TTFNSAT AV    F 
Sbjct: 309 VIYVTSGVYEENVVIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFV 368

Query: 370 GVNLTITNTAGAAKHQAVALRVSADMTTFYTCIFEGYQDTLYTHSLRQFYRECDIYGTVD 429
            VN+T  NTAG  KHQAVA+R SAD++ FY+C FE YQDTLYTHSLRQFYRECDIYGTVD
Sbjct: 369 AVNMTFRNTAGPEKHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVD 428

Query: 430 FIFGNAAVVFQTCNIYARLPLSGQFNALTAQGRTDPNQNTGTSIHNCTLKPTAELAASP- 489
           FIFGNAAVVFQ CN+Y R P+  QFNA+TAQGRTDPNQNTG SIHNCT+KP  +L +S  
Sbjct: 429 FIFGNAAVVFQDCNLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVSSNY 488

Query: 490 TTKSYLGRPWKQYSRTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSH 549
           T K+YLGRPWK+YSRTV+MQS+ID  ++P GW+EW+G   L+T YYAE+NN+G GS T+ 
Sbjct: 489 TVKTYLGRPWKEYSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTD 548

Query: 550 RVQW-AVGAINATVANNFTVSNFLAGDQWLPPTTVPYTGSLIS 562
           RV W     IN+T ANNFTV NFL GD W+  + VPY   L+S
Sbjct: 549 RVVWPGYHVINSTDANNFTVENFLLGDGWMVQSGVPYISGLLS 573

BLAST of Lag0007992 vs. ExPASy Swiss-Prot
Match: Q9FF77 (Probable pectinesterase/pectinesterase inhibitor 47 OS=Arabidopsis thaliana OX=3702 GN=PME47 PE=2 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 2.6e-114
Identity = 231/547 (42.23%), Postives = 328/547 (59.96%), Query Frame = 0

Query: 30  PGPVPTEEICRSTTSPFFCKSFL---PNSPATVHSHCRYTLRHALVHARTFLALV----- 89
           P P+P    C+ST  P  C++ L    +SP+  + + ++T++  L  AR    ++     
Sbjct: 79  PSPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQ 138

Query: 90  ----DTHITLFPFLSALQDCRSLAAANLDFISNSFHTVNTTSQALPFYQADEMLSLLSAV 149
               D   +    +SA+ DC  LA  +++++      +   ++ +     D + SLL  V
Sbjct: 139 RVEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELK-AAELMTAALVDRVTSLLGGV 198

Query: 150 ITNEDTCYEGLVGLGVGLGLVDKVLKAISFDRKLYSLYLSLFKLGWVSDDVKAPELPKTN 209
           +TN+ TC +GLV      G    +   +    +LYS+ L L     ++ ++K  +  K  
Sbjct: 199 VTNQQTCLDGLV--DAKSGFATAIGTPLGNLTRLYSVSLGLVSHA-LNRNLKRYKGSKGK 258

Query: 210 RFGLGNGALKLKMSPKDRAYYERLVHRK--KPAARRLLQTNNYENDGIVINGIVGVDQNG 269
            FG GN  ++  +    +   +     K  + A R L +        I++   V V    
Sbjct: 259 IFGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVTVGPYE 318

Query: 270 TYDFTTINAAIAAAPNNSVAAKGYYLIFVTAGIYNETVLVPKNKKYILMIGEGNNQTIIT 329
           T +F TI  A+AAAPN++   +GY++I+  AG+Y E V++   K+ I++IG+G N+TII+
Sbjct: 319 TDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTIIS 378

Query: 330 GNKNVVDGSTTFNSATVAVEGAGFFGVNLTITNTAGAAKHQAVALRVSADMTTFYTCIFE 389
           GN + +DG TT+NS+T AV G  F  V++T  NTAG  KHQAVA+R +AD +TFY C FE
Sbjct: 379 GNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSFE 438

Query: 390 GYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQTCNIYARLPLSGQFNALTAQGRTD 449
           GYQDTLY HSLRQFYRECDIYGT+DFIFGNAA +FQ CNIYAR P++ Q NA+TA GRTD
Sbjct: 439 GYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRTD 498

Query: 450 PNQNTGTSIHNCTLKPTAELAASP-TTKSYLGRPWKQYSRTVYMQSFIDAFIDPAGWKEW 509
           PNQ TG SI NCT+    +LAA P +T ++LGRPWK YSRTVY+QS+I   + P GW EW
Sbjct: 499 PNQKTGISIINCTIGAAPDLAADPKSTMTFLGRPWKPYSRTVYIQSYISDVVQPVGWLEW 558

Query: 510 DGTLNLNTSYYAEFNNSGPGSDTSHRVQWA-VGAINATVANNFTVSNFLAGDQWLPPTTV 561
           +GT  L+T  Y E++N GPG+DTS RVQW+    +N   A NFTV NF  GD WLP T +
Sbjct: 559 NGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTWLPQTDI 618

BLAST of Lag0007992 vs. ExPASy Swiss-Prot
Match: Q94CB1 (Probable pectinesterase/pectinesterase inhibitor 25 OS=Arabidopsis thaliana OX=3702 GN=PME25 PE=2 SV=1)

HSP 1 Score: 409.8 bits (1052), Expect = 4.8e-113
Identity = 232/561 (41.35%), Postives = 335/561 (59.71%), Query Frame = 0

Query: 17  LFLFSSSSSAVPVPGPVPTEEICRSTTSPFFCKSFL---PNSPATVHSHCRYTLRHALVH 76
           +F+  S   ++P          C+ST  P  C++ L    +SP+  + + ++T++  L  
Sbjct: 61  IFVPPSQPPSLPPSQSQSPSLACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQ 120

Query: 77  ARTFLALVDTHI---------TLFPFLSALQDCRSLAAANLDFISNSFHTVNTTSQALPF 136
           A     ++ ++               + A+ DC  L+  +++++  +  T   T+Q +  
Sbjct: 121 ASRLSKVITSYARRVESKPGSATAEEIGAVADCGELSELSVNYL-ETVTTELKTAQVMTA 180

Query: 137 YQADEMLSLLSAVITNEDTCYEGLVGLGVGLGLVDKVLKAISFDRKLYSLYLSLFKLGWV 196
              + + SLLS V+TN+ TC +GLV    G      +   +    +LYS+ L L     +
Sbjct: 181 ALVEHVNSLLSGVVTNQQTCLDGLVEAKSGFAAA--IGSPMGNLTRLYSISLGLVSHA-L 240

Query: 197 SDDVKAPELPKTNRFGLGNGALKLKMSPKDRAYYERLVHRK--KPAARRLLQTNNYENDG 256
           + ++K  +  K    G GN   +  +    +   +   + K  +  +R L +        
Sbjct: 241 NRNLKRFKASKGKILGGGNSTYREPLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGS 300

Query: 257 IVINGIVGVDQNGTYDFTTINAAIAAAPNNSVAAKGYYLIFVTAGIYNETVLVPKNKKYI 316
           I+++  V V    + +FTTI  AIAAAPNN+    GY++I+   G+Y E ++VP NKK +
Sbjct: 301 ILVSKAVIVGPFKSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNL 360

Query: 317 LMIGEGNNQTIITGNKNVVDGSTTFNSATVAVEGAGFFGVNLTITNTAGAAKHQAVALRV 376
           +++G+G N+TIITGN NVVDG TT+N ++ AV G  F  V++T  NTAG  KHQAVALR 
Sbjct: 361 MLMGDGINKTIITGNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRN 420

Query: 377 SADMTTFYTCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQTCNIYARLPLS 436
           +A+ ++FY C FEGYQDTLY HSLRQFYRECDIYGTVDFIFGNAA +FQ CNIYAR P++
Sbjct: 421 NAEGSSFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMA 480

Query: 437 GQFNALTAQGRTDPNQNTGTSIHNCTLKPTAELAASP-TTKSYLGRPWKQYSRTVYMQSF 496
            Q NA+TA GR DPNQNTG SI NCT+K   +LAA P +  ++LGRPWK YSRTV+MQS+
Sbjct: 481 KQKNAITAHGRLDPNQNTGISIINCTIKAAPDLAAEPKSAMTFLGRPWKPYSRTVFMQSY 540

Query: 497 IDAFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSHRVQW-AVGAINATVANNFTVSN 556
           I   + P GW EW+GT+ L+T YY E++N GPG++T+ RVQW     +N   A NFTV N
Sbjct: 541 ISDIVQPVGWLEWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYN 600

Query: 557 FLAGDQWLPPTTVPYTGSLIS 562
           F  GD WLP T +P+ G L+S
Sbjct: 601 FTMGDTWLPQTDIPFYGGLLS 617

BLAST of Lag0007992 vs. ExPASy TrEMBL
Match: A0A6J1EJM8 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111434929 PE=3 SV=1)

HSP 1 Score: 903.7 bits (2334), Expect = 3.9e-259
Identity = 453/561 (80.75%), Postives = 494/561 (88.06%), Query Frame = 0

Query: 1   MASSNSIPPLTILTLLLFLFSSSSSAVPVPGPVPTEEICRSTTSPFFCKSFLPNSPATVH 60
           MASS++   L +   LLFLF+SSS+       VPT E+C+S TSP FCKSFLP+SPA+VH
Sbjct: 1   MASSSNPISLLLFFSLLFLFTSSSA-------VPTVEVCQSATSPSFCKSFLPSSPASVH 60

Query: 61  SHCRYTLRHALVHARTFLALVDTHITLFPFLSALQDCRSLAAANLDFISNSFHTVNTTSQ 120
           SHCRYTL HALV ARTF+ LVD  +TLFP LSAL DCRSLA ANLDFIS++  TVNTT+ 
Sbjct: 61  SHCRYTLHHALVQARTFVTLVDLQLTLFPSLSALHDCRSLAVANLDFISSTVDTVNTTTN 120

Query: 121 ALPFYQADEMLSLLSAVITNEDTCYEGLVGLGVGLGLVDKVLKAISFDRKLYSLYLSLFK 180
           ALPFYQADEM+SLLSAVITN DTC++GL G G+GLG VDKV+KAISFDR+LYSLYLSLFK
Sbjct: 121 ALPFYQADEMISLLSAVITNIDTCHDGLAGSGLGLGFVDKVVKAISFDRQLYSLYLSLFK 180

Query: 181 LGWVSDDVKAPELPKTNRFGLGNGALKLKMSPKDRAYYERLVHRKKPAARRLLQTNNYEN 240
           +GWVS ++KAP+LPKTN FG G G LKLKMSPKDRAYYERLVH KKPAARRLLQT NY +
Sbjct: 181 MGWVSKNMKAPKLPKTNHFGFGKGPLKLKMSPKDRAYYERLVHGKKPAARRLLQT-NYAD 240

Query: 241 DGIVINGIVGVDQNGTYDFTTINAAIAAAPNNSVAAKGYYLIFVTAGIYNETVLVPKNKK 300
           DGI++NG+V VDQNG YDFTTIN AIAAAPN S AA GY+LIFVTAGIY E+V+VPK+KK
Sbjct: 241 DGILVNGVVAVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVVVPKSKK 300

Query: 301 YILMIGEGNNQTIITGNKNVVDGSTTFNSATVAVEGAGFFGVNLTITNTAGAAKHQAVAL 360
           Y+LMIGEGNN+TIITGNKNVVDGSTTFNSATV VEG GFFG NLTITNTAGA KHQAVAL
Sbjct: 301 YVLMIGEGNNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIKHQAVAL 360

Query: 361 RVSADMTTFYTCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQTCNIYARLP 420
           RVSADMTTFY+CIFEGYQDTLY HSLRQFYRECDIYGTVDFIFGNAAVV Q CNIYARLP
Sbjct: 361 RVSADMTTFYSCIFEGYQDTLYAHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLP 420

Query: 421 LSGQFNALTAQGRTDPNQNTGTSIHNCTLKPTAELAASPTTKSYLGRPWKQYSRTVYMQS 480
           LSGQFNALTAQGRTD NQNTG  IH CT+KPT +LAASPTTKSYLGRPWK++SRTVYMQS
Sbjct: 421 LSGQFNALTAQGRTDQNQNTGIFIHKCTIKPTPDLAASPTTKSYLGRPWKEFSRTVYMQS 480

Query: 481 FIDAFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSHRVQWAVGAINATVANNFTVSN 540
           FIDAFIDPAGWKEWDGTLNLNTSYYAEF+NSGPGSDTS R QWAVG INATVAN FTVS 
Sbjct: 481 FIDAFIDPAGWKEWDGTLNLNTSYYAEFDNSGPGSDTSQRAQWAVGVINATVANIFTVSE 540

Query: 541 FLAGDQWLPPTTVPYTGSLIS 562
            LAGDQWLPPT VPY+G LIS
Sbjct: 541 ILAGDQWLPPTAVPYSGGLIS 553

BLAST of Lag0007992 vs. ExPASy TrEMBL
Match: A0A6J1L6X0 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111499728 PE=3 SV=1)

HSP 1 Score: 902.5 bits (2331), Expect = 8.8e-259
Identity = 452/565 (80.00%), Postives = 495/565 (87.61%), Query Frame = 0

Query: 1   MASSNSIPPLTILTLLLFLFSSSSSAVPVPGPVPTEEICRSTTSPFFCKSFLPNSPATVH 60
           M SS++IP LT LT LL+LFS SSSA  +  PVP  +IC STTSP FCK+FLPNS ATVH
Sbjct: 1   MGSSSAIPLLTFLT-LLYLFSFSSSA--IHSPVPAADICHSTTSPSFCKAFLPNSLATVH 60

Query: 61  SHCRYTLRHALVHARTFLALVDTHITLFPFLSALQDCRSLAAANLDFISNSFHTVN---- 120
           SHCR+TLRHAL HARTFLALVDTH+TLFP L+AL DCRSLAAANLDF+SN+F TVN    
Sbjct: 61  SHCRFTLRHALAHARTFLALVDTHLTLFPSLAALHDCRSLAAANLDFLSNTFPTVNTTTT 120

Query: 121 TTSQALPFYQADEMLSLLSAVITNEDTCYEGLVGLGVGLGLVDKVLKAISFDRKLYSLYL 180
           TT+ ALPFY+A+EM+SLLSAVITNE+TCYEGL+  G   GLVDKVLKAISFD KLYSLYL
Sbjct: 121 TTTNALPFYKAEEMVSLLSAVITNENTCYEGLMSSGSASGLVDKVLKAISFDTKLYSLYL 180

Query: 181 SLFKLGWVSDDVKAPELPKTNRFGLGNGALKLKMSPKDRAYYERLVHRKKPAARRLLQTN 240
           SLFK+GWVS+DVKAP +PK   FG G G LKL MSPK+RAYYERL+HR KP ARRLLQTN
Sbjct: 181 SLFKMGWVSNDVKAPHVPKMKHFGRGKGQLKLNMSPKNRAYYERLMHRSKPPARRLLQTN 240

Query: 241 NYENDGIVINGIVGVDQNGTYDFTTINAAIAAAPNNSVAAKGYYLIFVTAGIYNETVLVP 300
           + E+DGI++NGIV VDQNGTYDFTTINAAI AAPNNSVAA GY+LI VTAG+YNE+V+VP
Sbjct: 241 SNEDDGILVNGIVAVDQNGTYDFTTINAAIQAAPNNSVAADGYFLIIVTAGVYNESVMVP 300

Query: 301 KNKKYILMIGEGNNQTIITGNKNVVDGSTTFNSATVAVEGAGFFGVNLTITNTAGAAKHQ 360
           K+KKY+LMIGEGNNQTIITGNKNVVDGSTTFNS T  VEG GFFGVNLTITNTAG  K Q
Sbjct: 301 KHKKYVLMIGEGNNQTIITGNKNVVDGSTTFNSGTFIVEGTGFFGVNLTITNTAGPNKFQ 360

Query: 361 AVALRVSADMTTFYTCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQTCNIY 420
           AVALRVSADMTTFY+CIFEGYQDTLY HSLRQFYRECDIYGT+DFI G+AAVVFQ CNIY
Sbjct: 361 AVALRVSADMTTFYSCIFEGYQDTLYPHSLRQFYRECDIYGTIDFILGDAAVVFQNCNIY 420

Query: 421 ARLPLSGQFNALTAQGRTDPNQNTGTSIHNCTLKPTAELAASPTTKSYLGRPWKQYSRTV 480
           ARLP+ GQ+  +TAQGRTD NQNTG SIHNCT+KPTA+LAASPT KSY GRPWK+Y+RTV
Sbjct: 421 ARLPMKGQYIVITAQGRTDRNQNTGISIHNCTIKPTAQLAASPTIKSYFGRPWKEYARTV 480

Query: 481 YMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSHRVQWAVGAINATVANNF 540
           YMQSFIDAFIDP GW  WDGTLNLNTSYYAEFNNSGPGSDTS R +WAVG INATVANNF
Sbjct: 481 YMQSFIDAFIDPEGWNVWDGTLNLNTSYYAEFNNSGPGSDTSRRAKWAVGLINATVANNF 540

Query: 541 TVSNFLAGDQWLPPTTVPYTGSLIS 562
           TVS FL GDQWLPPTTVPYTG LIS
Sbjct: 541 TVSPFLGGDQWLPPTTVPYTGGLIS 562

BLAST of Lag0007992 vs. ExPASy TrEMBL
Match: A0A5A7TRW1 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold299G002210 PE=3 SV=1)

HSP 1 Score: 900.2 bits (2325), Expect = 4.4e-258
Identity = 455/563 (80.82%), Postives = 489/563 (86.86%), Query Frame = 0

Query: 1   MASSNSIPPLTILTLLLFLFSSSSSAVPVPGPVPTEEICRSTTSPFFCKSFLPNSPATVH 60
           M SSN I  LT LTLL FL  SSS+       VPT EIC+STTSP FC  FLPNSPA+VH
Sbjct: 1   MVSSNPISVLTFLTLLFFLSPSSSA-------VPTAEICQSTTSPPFCNFFLPNSPASVH 60

Query: 61  SHCRYTLRHALVHARTFLALVDTHITLFPFLSALQDCRSLAAANLDFISNSFHTVN-TTS 120
           SHCR+TL HALVHARTFL+LV+ H+ LFP LS L DCRSLA ANLDF+S +F  VN TT+
Sbjct: 61  SHCRFTLHHALVHARTFLSLVNAHLNLFPSLSPLHDCRSLAEANLDFLSQTFSIVNSTTT 120

Query: 121 QALPFYQADEMLSLLSAVITNEDTCYEGLVGLGVGLGLVDKVLKAISFDRKLYSLYLSLF 180
             LP+Y A E L+L+SAVITNEDTCYEGL GL   +GLVDKVL+AISFD+KLYSLYLSLF
Sbjct: 121 TLLPYYDAHEKLTLMSAVITNEDTCYEGLAGLDSAVGLVDKVLEAISFDKKLYSLYLSLF 180

Query: 181 KLGWVSDDVKAPELPKTNRFGLGNGALKLKMSPKDRAYYERLVHRKK-PAARRLLQTNNY 240
           K+GWVS D+KAP LPK   FG+G G LKLKMSPKDRAYYERLVHR K P ARRLLQT NY
Sbjct: 181 KMGWVSKDLKAPTLPKMKHFGVGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQT-NY 240

Query: 241 ENDGIVINGIVGVDQNGTYDFTTINAAIAAAPNNSVAAKGYYLIFVTAGIYNETVLVPKN 300
           ++DGIVINGIVGVDQNGTYDFT I AAIAAAPN +  AKGY+LIFV AGIYNETVL+PK 
Sbjct: 241 QDDGIVINGIVGVDQNGTYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLIPKE 300

Query: 301 KKYILMIGEGNNQTIITGNKNVVDGSTTFNSATVAVEGAGFFGVNLTITNTAGAAKHQAV 360
           K+Y+L+IGEGNNQTIITGNKNVVDGSTTFNSATVAVEG GF GVNLTI+NTAGAAKHQAV
Sbjct: 301 KRYVLLIGEGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTISNTAGAAKHQAV 360

Query: 361 ALRVSADMTTFYTCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQTCNIYAR 420
           ALRVSAD  T Y CIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVV Q CNIYAR
Sbjct: 361 ALRVSADNATLYNCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYAR 420

Query: 421 LPLSGQFNALTAQGRTDPNQNTGTSIHNCTLKPTAELAASPTTKSYLGRPWKQYSRTVYM 480
           LPLSGQFNA+TAQGRTDPNQNTGTSIHNCT+K T ELAASP TKSYLGRPWKQYSRTVYM
Sbjct: 421 LPLSGQFNAVTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYM 480

Query: 481 QSFIDAFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSHRVQWAVGAINATVANNFTV 540
           QSFID+FIDPAGWKEWDGT+NLNTSYYAEFNNSGPGSDTS RV WAVG INATVAN+FTV
Sbjct: 481 QSFIDSFIDPAGWKEWDGTMNLNTSYYAEFNNSGPGSDTSQRVSWAVGVINATVANSFTV 540

Query: 541 SNFLAGDQWLPPTTVPYTGSLIS 562
           S FLAGD+WLPPT VPYTG LIS
Sbjct: 541 SQFLAGDRWLPPTDVPYTGGLIS 555

BLAST of Lag0007992 vs. ExPASy TrEMBL
Match: A0A1S3C644 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103497470 PE=3 SV=1)

HSP 1 Score: 900.2 bits (2325), Expect = 4.4e-258
Identity = 455/563 (80.82%), Postives = 489/563 (86.86%), Query Frame = 0

Query: 1   MASSNSIPPLTILTLLLFLFSSSSSAVPVPGPVPTEEICRSTTSPFFCKSFLPNSPATVH 60
           M SSN I  LT LTLL FL  SSS+       VPT EIC+STTSP FC  FLPNSPA+VH
Sbjct: 1   MVSSNPISVLTFLTLLFFLSPSSSA-------VPTAEICQSTTSPPFCNFFLPNSPASVH 60

Query: 61  SHCRYTLRHALVHARTFLALVDTHITLFPFLSALQDCRSLAAANLDFISNSFHTVN-TTS 120
           SHCR+TL HALVHARTFL+LV+ H+ LFP LS L DCRSLA ANLDF+S +F  VN TT+
Sbjct: 61  SHCRFTLHHALVHARTFLSLVNAHLNLFPSLSPLHDCRSLAEANLDFLSQTFSIVNSTTT 120

Query: 121 QALPFYQADEMLSLLSAVITNEDTCYEGLVGLGVGLGLVDKVLKAISFDRKLYSLYLSLF 180
             LP+Y A E L+L+SAVITNEDTCYEGL GL   +GLVDKVL+AISFD+KLYSLYLSLF
Sbjct: 121 TLLPYYDAHEKLTLMSAVITNEDTCYEGLAGLDSAVGLVDKVLEAISFDKKLYSLYLSLF 180

Query: 181 KLGWVSDDVKAPELPKTNRFGLGNGALKLKMSPKDRAYYERLVHRKK-PAARRLLQTNNY 240
           K+GWVS D+KAP LPK   FG+G G LKLKMSPKDRAYYERLVHR K P ARRLLQT NY
Sbjct: 181 KMGWVSKDLKAPTLPKMKHFGVGKGQLKLKMSPKDRAYYERLVHRNKPPGARRLLQT-NY 240

Query: 241 ENDGIVINGIVGVDQNGTYDFTTINAAIAAAPNNSVAAKGYYLIFVTAGIYNETVLVPKN 300
           ++DGIVINGIVGVDQNGTYDFT I AAIAAAPN +  AKGY+LIFV AGIYNETVL+PK 
Sbjct: 241 QDDGIVINGIVGVDQNGTYDFTNITAAIAAAPNKTTVAKGYFLIFVAAGIYNETVLIPKE 300

Query: 301 KKYILMIGEGNNQTIITGNKNVVDGSTTFNSATVAVEGAGFFGVNLTITNTAGAAKHQAV 360
           K+Y+L+IGEGNNQTIITGNKNVVDGSTTFNSATVAVEG GF GVNLTI+NTAGAAKHQAV
Sbjct: 301 KRYVLLIGEGNNQTIITGNKNVVDGSTTFNSATVAVEGTGFLGVNLTISNTAGAAKHQAV 360

Query: 361 ALRVSADMTTFYTCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQTCNIYAR 420
           ALRVSAD  T Y CIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVV Q CNIYAR
Sbjct: 361 ALRVSADNATLYNCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYAR 420

Query: 421 LPLSGQFNALTAQGRTDPNQNTGTSIHNCTLKPTAELAASPTTKSYLGRPWKQYSRTVYM 480
           LPLSGQFNA+TAQGRTDPNQNTGTSIHNCT+K T ELAASP TKSYLGRPWKQYSRTVYM
Sbjct: 421 LPLSGQFNAVTAQGRTDPNQNTGTSIHNCTIKATPELAASPATKSYLGRPWKQYSRTVYM 480

Query: 481 QSFIDAFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSHRVQWAVGAINATVANNFTV 540
           QSFID+FIDPAGWKEWDGT+NLNTSYYAEFNNSGPGSDTS RV WAVG INATVAN+FTV
Sbjct: 481 QSFIDSFIDPAGWKEWDGTMNLNTSYYAEFNNSGPGSDTSQRVSWAVGVINATVANSFTV 540

Query: 541 SNFLAGDQWLPPTTVPYTGSLIS 562
           S FLAGD+WLPPT VPYTG LIS
Sbjct: 541 SQFLAGDRWLPPTDVPYTGGLIS 555

BLAST of Lag0007992 vs. ExPASy TrEMBL
Match: A0A6J1I6B8 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111470332 PE=3 SV=1)

HSP 1 Score: 896.3 bits (2315), Expect = 6.3e-257
Identity = 453/561 (80.75%), Postives = 493/561 (87.88%), Query Frame = 0

Query: 2   ASSNSIPPLTILTLL-LFLFSSSSSAVPVPGPVPTEEICRSTTSPFFCKSFLPNSPATVH 61
           +SSN I  LT  +LL LFLF+SSS+       VPT E+C+S TSP FCKSFLP+SPA+VH
Sbjct: 3   SSSNPISLLTFFSLLFLFLFTSSSA-------VPTVEVCQSATSPSFCKSFLPSSPASVH 62

Query: 62  SHCRYTLRHALVHARTFLALVDTHITLFPFLSALQDCRSLAAANLDFISNSFHTVNTTSQ 121
           SHCRYTL HALV ARTF+ LVD  +TL P  SAL DCRSLA ANLDFIS++ +TVNTT+ 
Sbjct: 63  SHCRYTLHHALVQARTFITLVDLQLTLLPSFSALHDCRSLAMANLDFISSTVNTVNTTTN 122

Query: 122 ALPFYQADEMLSLLSAVITNEDTCYEGLVGLGVGLGLVDKVLKAISFDRKLYSLYLSLFK 181
           ALPFYQADEM+SLLSAVITN DTC++GL   G GLGLVDKV+KAISFDR+LYSLYLSLFK
Sbjct: 123 ALPFYQADEMISLLSAVITNVDTCHDGLA--GSGLGLVDKVVKAISFDRQLYSLYLSLFK 182

Query: 182 LGWVSDDVKAPELPKTNRFGLGNGALKLKMSPKDRAYYERLVHRKKPAARRLLQTNNYEN 241
           +GWVS  +KAP+LPKTN FG GNG LKLKMSPKDRAYYERL+H KKPAARRLLQTNN  +
Sbjct: 183 MGWVSKTMKAPKLPKTNHFGFGNGPLKLKMSPKDRAYYERLMHGKKPAARRLLQTNN-AD 242

Query: 242 DGIVINGIVGVDQNGTYDFTTINAAIAAAPNNSVAAKGYYLIFVTAGIYNETVLVPKNKK 301
           DGI++NG+V VDQNG YDFTTIN AIAAAPN S AA GY+LIFVTAGIY E+V+VPK+KK
Sbjct: 243 DGILVNGVVAVDQNGAYDFTTINDAIAAAPNKSTAANGYFLIFVTAGIYMESVVVPKSKK 302

Query: 302 YILMIGEGNNQTIITGNKNVVDGSTTFNSATVAVEGAGFFGVNLTITNTAGAAKHQAVAL 361
           Y+LMIGEGNN+TIITGNKNVVDGSTTFNSATV VEG GFFG NLTITNTAGA KHQAVAL
Sbjct: 303 YVLMIGEGNNRTIITGNKNVVDGSTTFNSATVIVEGTGFFGANLTITNTAGAIKHQAVAL 362

Query: 362 RVSADMTTFYTCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQTCNIYARLP 421
           RVSADMTTFY+CIFEGYQDTLY HSLRQFYRECDIYGTVDFIFGNAAVV Q CNIYARLP
Sbjct: 363 RVSADMTTFYSCIFEGYQDTLYAHSLRQFYRECDIYGTVDFIFGNAAVVLQNCNIYARLP 422

Query: 422 LSGQFNALTAQGRTDPNQNTGTSIHNCTLKPTAELAASPTTKSYLGRPWKQYSRTVYMQS 481
           LSGQFNALTAQGRTD NQNTG  IH CT+KPT +LAASPTTKSYLGRPWK++SRTVYMQS
Sbjct: 423 LSGQFNALTAQGRTDQNQNTGIFIHKCTIKPTPDLAASPTTKSYLGRPWKEFSRTVYMQS 482

Query: 482 FIDAFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSHRVQWAVGAINATVANNFTVSN 541
           FIDAFIDPAGWKEWDGTLNLNTSYYAEF+NSGPGSDTS R QWAVG INATVAN FTVS 
Sbjct: 483 FIDAFIDPAGWKEWDGTLNLNTSYYAEFDNSGPGSDTSRRAQWAVGVINATVANIFTVSE 542

Query: 542 FLAGDQWLPPTTVPYTGSLIS 562
            LAGDQWLPPT VPY+G LIS
Sbjct: 543 ILAGDQWLPPTAVPYSGGLIS 553

BLAST of Lag0007992 vs. TAIR 10
Match: AT2G47550.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 534.6 bits (1376), Expect = 9.3e-152
Identity = 288/570 (50.53%), Postives = 378/570 (66.32%), Query Frame = 0

Query: 16  LLFLFSSS----------SSAVPVPGPVPT-EEICRSTTSPFFCKSFLPNSPATVHSHCR 75
           L+FLF+ +          S+ +P  G   +   ICR    P +C+S LPN P  ++S+ R
Sbjct: 5   LMFLFTLACLSSLPSPFISAQIPAIGNATSPSNICRFAPDPSYCRSVLPNQPGDIYSYGR 64

Query: 76  YTLRHALVHARTFLALVDTH------ITLFPFLSALQDCRSLAAANLDFISNSFHTVNTT 135
            +LR +L  AR F++++D        +     + AL+DC+ LA+  +D++ +S  T ++T
Sbjct: 65  LSLRRSLSRARRFISMIDAELDRKGKVAAKSTVGALEDCKFLASLTMDYLLSSSQTADST 124

Query: 136 SQALPFYQADEMLSLLSAVITNEDTCYEGLVGLGVGLGLVDKVLKAISFDRKLYSLYLSL 195
            + L   +A+++ + LSA ITNE TC EGL       GL   +      D KLY + L+L
Sbjct: 125 -KTLSLSRAEDVHTFLSAAITNEQTCLEGLKSTASENGLSGDLFN----DTKLYGVSLAL 184

Query: 196 FKLGWV-----SDDVKAPELPKTNRFGLGNGALKLKMSPKDRAYYERLVHRKKPAARRLL 255
           F  GWV     S  +  P+      FG  NG L LKM+ + RA Y  +  RK      LL
Sbjct: 185 FSKGWVPRRQRSRPIWQPQARFKKFFGFRNGKLPLKMTERARAVYNTVTRRK------LL 244

Query: 256 QTNNYENDGIVINGIVGVDQNGTYDFTTINAAIAAAPNNSVAAKGYYLIFVTAGIYNETV 315
           Q+   + D + ++ IV V QNGT +FTTINAAIAAAPN +  + GY+LI+VTAG+Y E V
Sbjct: 245 QS---DADAVQVSDIVTVIQNGTGNFTTINAAIAAAPNKTDGSNGYFLIYVTAGLYEEYV 304

Query: 316 LVPKNKKYILMIGEGNNQTIITGNKNVVDGSTTFNSATVAVEGAGFFGVNLTITNTAGAA 375
            VPKNK+Y++MIG+G NQT+ITGN++VVDG TTFNSAT  + G  F GVN+TI NTAG  
Sbjct: 305 EVPKNKRYVMMIGDGINQTVITGNRSVVDGWTTFNSATFILSGPNFIGVNITIRNTAGPT 364

Query: 376 KHQAVALRVSADMTTFYTCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQTC 435
           K QAVALR   D++ FY+C FE YQDTLYTHSLRQFYRECD+YGTVDFIFGNAAVV Q C
Sbjct: 365 KGQAVALRSGGDLSVFYSCSFEAYQDTLYTHSLRQFYRECDVYGTVDFIFGNAAVVLQNC 424

Query: 436 NIYARLPLSGQFNALTAQGRTDPNQNTGTSIHNCTLKPTAELAASP-TTKSYLGRPWKQY 495
           N+Y R P  GQ N +TAQGRTDPNQNTGT+IH CT++P  +LA S  T K+YLGRPWK+Y
Sbjct: 425 NLYPRQPRKGQSNEVTAQGRTDPNQNTGTAIHGCTIRPADDLATSNYTVKTYLGRPWKEY 484

Query: 496 SRTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSHRVQW-AVGAINAT 555
           SRTV MQ++ID F++P+GW  W G   L+T YYAE+NN+GPGSDT++RV W     INAT
Sbjct: 485 SRTVVMQTYIDGFLEPSGWNAWSGDFALSTLYYAEYNNTGPGSDTTNRVTWPGYHVINAT 544

Query: 556 VANNFTVSNFLAGDQWLPPTTVPYTGSLIS 562
            A+NFTV+NFL G+ W+  T VP+ G LI+
Sbjct: 545 DASNFTVTNFLVGEGWIGQTGVPFVGGLIA 560

BLAST of Lag0007992 vs. TAIR 10
Match: AT1G02810.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 523.5 bits (1347), Expect = 2.1e-148
Identity = 297/591 (50.25%), Postives = 380/591 (64.30%), Query Frame = 0

Query: 8   PPLTILTLLLFLFSSSSSAVPVPGPVPTEEICRSTTSPFFCKSFLPNSPATVHSHCRYTL 67
           P   ++TL  FL S  +S+  +     +  IC++T  P +CKS  P+S   V  +  +++
Sbjct: 4   PIFILITLSFFLQSVLASSQTLSN---SSTICKTTPDPKYCKSVFPHSQGNVQQYGCFSI 63

Query: 68  RHALVHARTFLALVDTHITLFPFLS------ALQDCRSLAAANLDFISNSFHTVNTTS-- 127
           R +L  +R F+  VD +I     LS      ALQDCR LA   +D++  SF TVN TS  
Sbjct: 64  RKSLSQSRKFIRTVDRYIKRNAHLSQPAVIRALQDCRFLAGLTMDYLLTSFETVNDTSAK 123

Query: 128 ---QALPFYQADEMLSLLSAVITNEDTCYEGL-----------VGLGVGLGLVDKVLKAI 187
              + L F +AD++ +LLSA +TNE TC EGL           V  GV L LV+      
Sbjct: 124 TSFKPLSFPKADDIQTLLSAALTNEQTCLEGLTTAASYSATWTVRTGVALPLVN------ 183

Query: 188 SFDRKLYSLYLSLFKLGWVSDDVK----------APELPKTNRFGL-GNGALKLKMSPKD 247
             D KL  + L+LF  GWV    K          +     T  F L  NGAL LKM+ K 
Sbjct: 184 --DTKLLGVSLALFTKGWVPKKKKRAGFAWAQPRSGSSTHTKPFRLFRNGALPLKMTEKT 243

Query: 248 RAYYERLVHRKKPAARRLLQTNNYENDG--IVINGIVGVDQNGTYDFTTINAAIAAAPNN 307
           +A YE L  RK         +N   +DG  ++I+ IV V Q+GT +FT I AA+AAAPNN
Sbjct: 244 KAVYESLSRRKLADG----DSNGDGDDGSMVLISDIVTVSQDGTGNFTNITAAVAAAPNN 303

Query: 308 SVAAKGYYLIFVTAGIYNETVLVPKNKKYILMIGEGNNQTIITGNKNVVDGSTTFNSATV 367
           +  + G++LI+VTAGIY E + + KNK+Y++MIG+G NQT++TGN++VVDG TTFNSAT 
Sbjct: 304 TDGSAGFFLIYVTAGIYEEYISIAKNKRYMMMIGDGINQTVVTGNRSVVDGWTTFNSATF 363

Query: 368 AVEGAGFFGVNLTITNTAGAAKHQAVALRVSADMTTFYTCIFEGYQDTLYTHSLRQFYRE 427
           AV    F  VN+T  NTAG  KHQAVALR  AD + FY+C FE YQDTLYTHSLRQFYRE
Sbjct: 364 AVTAPNFVAVNITFRNTAGPEKHQAVALRSGADFSIFYSCSFEAYQDTLYTHSLRQFYRE 423

Query: 428 CDIYGTVDFIFGNAAVVFQTCNIYARLPLSGQFNALTAQGRTDPNQNTGTSIHNCTLKPT 487
           CD+YGTVDFIFGNAAVVFQ CN+Y R P+  QFNA+TAQGR+DPNQNTGTSI NCT+KP 
Sbjct: 424 CDVYGTVDFIFGNAAVVFQNCNLYPRKPMPNQFNAITAQGRSDPNQNTGTSIQNCTIKPA 483

Query: 488 AELAASP-TTKSYLGRPWKQYSRTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFNNS 547
            +L +S  T K+YLGRPWK+YSRTVYMQS+ID F++P GW+EW+G   L+T YYAE+NN+
Sbjct: 484 DDLVSSNYTVKTYLGRPWKEYSRTVYMQSYIDGFVEPVGWREWNGDFALSTLYYAEYNNT 543

Query: 548 GPGSDTSHRVQW-AVGAINATVANNFTVSNFLAGDQWLPPTTVPYTGSLIS 562
           GPGS+T++RV W     IN+T A NFTV+       W+  T VPYT  LIS
Sbjct: 544 GPGSNTTNRVTWPGYHVINSTDAANFTVTGLFIEADWIWKTGVPYTSGLIS 579

BLAST of Lag0007992 vs. TAIR 10
Match: AT4G02330.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 522.7 bits (1345), Expect = 3.6e-148
Identity = 291/583 (49.91%), Postives = 381/583 (65.35%), Query Frame = 0

Query: 10  LTILTLLLFLFSSSSSAVPVPGPVPTEEICRSTTSPFFCKSFLP-NSPATVHSHCRYTLR 69
           +T+   L  LF +S + +P      +  IC++T  P FCKS  P  S   V  + R++LR
Sbjct: 9   VTLFLSLQTLFIASQTLLPSNS---SSTICKTTPDPKFCKSVFPQTSQGDVREYGRFSLR 68

Query: 70  HALVHARTFLALVDTHITLFPFL------SALQDCRSLAAANLDFISNSFHTVN--TTSQ 129
            +L  +R F   +D ++     L       ALQDCR LA+   D++  SF TVN  T+S+
Sbjct: 69  KSLTQSRKFTRTIDRYLKRNNALLSQSAVGALQDCRYLASLTTDYLITSFETVNITTSSK 128

Query: 130 ALPFYQADEMLSLLSAVITNEDTCYEGL---------VGLGVGLGLVDKVLKAISFDRKL 189
            L F +ADE+ +LLSA +TNE TC +G+         +  GV L L++        D KL
Sbjct: 129 TLSFSKADEIQTLLSAALTNEQTCLDGINTAASSSWTIRNGVALPLIN--------DTKL 188

Query: 190 YSLYLSLFKLGWVSDDVKAPEL-----PK-----TNRF-GLGNGALKLKMSPKDRAYYER 249
           +S+ L+LF  GWV    K         PK     T  F    NGAL LKM+   RA YE 
Sbjct: 189 FSVSLALFTKGWVPKKKKQVASYSWAHPKNTHSHTKPFRHFRNGALPLKMTEHTRAVYES 248

Query: 250 LVHRKKPAARRLLQTNNYENDGIVINGIVGVDQNGTYDFTTINAAIAAAPNNSVAAKGYY 309
           L       +RR L  ++ + + ++++ IV V+QNGT +FTTI  A+ +APN +    GY+
Sbjct: 249 L-------SRRKLADDDNDVNTVLVSDIVTVNQNGTGNFTTITEAVNSAPNKTDGTAGYF 308

Query: 310 LIFVTAGIYNETVLVPKNKKYILMIGEGNNQTIITGNKNVVDGSTTFNSATVAVEGAGFF 369
           +I+VT+G+Y E V++ KNK+Y++MIG+G N+T++TGN+NVVDG TTFNSAT AV    F 
Sbjct: 309 VIYVTSGVYEENVVIAKNKRYLMMIGDGINRTVVTGNRNVVDGWTTFNSATFAVTSPNFV 368

Query: 370 GVNLTITNTAGAAKHQAVALRVSADMTTFYTCIFEGYQDTLYTHSLRQFYRECDIYGTVD 429
            VN+T  NTAG  KHQAVA+R SAD++ FY+C FE YQDTLYTHSLRQFYRECDIYGTVD
Sbjct: 369 AVNMTFRNTAGPEKHQAVAMRSSADLSIFYSCSFEAYQDTLYTHSLRQFYRECDIYGTVD 428

Query: 430 FIFGNAAVVFQTCNIYARLPLSGQFNALTAQGRTDPNQNTGTSIHNCTLKPTAELAASP- 489
           FIFGNAAVVFQ CN+Y R P+  QFNA+TAQGRTDPNQNTG SIHNCT+KP  +L +S  
Sbjct: 429 FIFGNAAVVFQDCNLYPRQPMQNQFNAITAQGRTDPNQNTGISIHNCTIKPADDLVSSNY 488

Query: 490 TTKSYLGRPWKQYSRTVYMQSFIDAFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSH 549
           T K+YLGRPWK+YSRTV+MQS+ID  ++P GW+EW+G   L+T YYAE+NN+G GS T+ 
Sbjct: 489 TVKTYLGRPWKEYSRTVFMQSYIDEVVEPVGWREWNGDFALSTLYYAEYNNTGSGSSTTD 548

Query: 550 RVQW-AVGAINATVANNFTVSNFLAGDQWLPPTTVPYTGSLIS 562
           RV W     IN+T ANNFTV NFL GD W+  + VPY   L+S
Sbjct: 549 RVVWPGYHVINSTDANNFTVENFLLGDGWMVQSGVPYISGLLS 573

BLAST of Lag0007992 vs. TAIR 10
Match: AT5G04970.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 414.1 bits (1063), Expect = 1.8e-115
Identity = 231/547 (42.23%), Postives = 328/547 (59.96%), Query Frame = 0

Query: 30  PGPVPTEEICRSTTSPFFCKSFL---PNSPATVHSHCRYTLRHALVHARTFLALV----- 89
           P P+P    C+ST  P  C++ L    +SP+  + + ++T++  L  AR    ++     
Sbjct: 79  PSPLPPNIACKSTPYPKLCRTILSAVKSSPSDPYHYGKFTMKQCLKQARRLSKVINRFAQ 138

Query: 90  ----DTHITLFPFLSALQDCRSLAAANLDFISNSFHTVNTTSQALPFYQADEMLSLLSAV 149
               D   +    +SA+ DC  LA  +++++      +   ++ +     D + SLL  V
Sbjct: 139 RVEADPGTSTVEEVSAVADCGELAELSVEYLETVTEELK-AAELMTAALVDRVTSLLGGV 198

Query: 150 ITNEDTCYEGLVGLGVGLGLVDKVLKAISFDRKLYSLYLSLFKLGWVSDDVKAPELPKTN 209
           +TN+ TC +GLV      G    +   +    +LYS+ L L     ++ ++K  +  K  
Sbjct: 199 VTNQQTCLDGLV--DAKSGFATAIGTPLGNLTRLYSVSLGLVSHA-LNRNLKRYKGSKGK 258

Query: 210 RFGLGNGALKLKMSPKDRAYYERLVHRK--KPAARRLLQTNNYENDGIVINGIVGVDQNG 269
            FG GN  ++  +    +   +     K  + A R L +        I++   V V    
Sbjct: 259 IFGGGNKPVREPLETLIKVLRKTCDKGKDCRKANRNLGELGETSGGSILVREAVTVGPYE 318

Query: 270 TYDFTTINAAIAAAPNNSVAAKGYYLIFVTAGIYNETVLVPKNKKYILMIGEGNNQTIIT 329
           T +F TI  A+AAAPN++   +GY++I+  AG+Y E V++   K+ I++IG+G N+TII+
Sbjct: 319 TDNFPTITEAVAAAPNHTFPEQGYFVIYARAGLYEEYVVISNKKRNIMLIGDGINKTIIS 378

Query: 330 GNKNVVDGSTTFNSATVAVEGAGFFGVNLTITNTAGAAKHQAVALRVSADMTTFYTCIFE 389
           GN + +DG TT+NS+T AV G  F  V++T  NTAG  KHQAVA+R +AD +TFY C FE
Sbjct: 379 GNHSFIDGWTTYNSSTFAVVGDRFVAVDVTFRNTAGPEKHQAVAVRNNADGSTFYRCSFE 438

Query: 390 GYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQTCNIYARLPLSGQFNALTAQGRTD 449
           GYQDTLY HSLRQFYRECDIYGT+DFIFGNAA +FQ CNIYAR P++ Q NA+TA GRTD
Sbjct: 439 GYQDTLYVHSLRQFYRECDIYGTIDFIFGNAAAIFQNCNIYARKPMANQKNAVTAHGRTD 498

Query: 450 PNQNTGTSIHNCTLKPTAELAASP-TTKSYLGRPWKQYSRTVYMQSFIDAFIDPAGWKEW 509
           PNQ TG SI NCT+    +LAA P +T ++LGRPWK YSRTVY+QS+I   + P GW EW
Sbjct: 499 PNQKTGISIINCTIGAAPDLAADPKSTMTFLGRPWKPYSRTVYIQSYISDVVQPVGWLEW 558

Query: 510 DGTLNLNTSYYAEFNNSGPGSDTSHRVQWA-VGAINATVANNFTVSNFLAGDQWLPPTTV 561
           +GT  L+T  Y E++N GPG+DTS RVQW+    +N   A NFTV NF  GD WLP T +
Sbjct: 559 NGTTGLDTISYGEYDNFGPGADTSKRVQWSGYSLLNLVQAMNFTVYNFTLGDTWLPQTDI 618

BLAST of Lag0007992 vs. TAIR 10
Match: AT3G10720.2 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 409.8 bits (1052), Expect = 3.4e-114
Identity = 232/561 (41.35%), Postives = 335/561 (59.71%), Query Frame = 0

Query: 17  LFLFSSSSSAVPVPGPVPTEEICRSTTSPFFCKSFL---PNSPATVHSHCRYTLRHALVH 76
           +F+  S   ++P          C+ST  P  C++ L    +SP+  + + ++T++  L  
Sbjct: 61  IFVPPSQPPSLPPSQSQSPSLACKSTPYPKLCRTILNAVKSSPSDPYRYGKFTIKQCLKQ 120

Query: 77  ARTFLALVDTHI---------TLFPFLSALQDCRSLAAANLDFISNSFHTVNTTSQALPF 136
           A     ++ ++               + A+ DC  L+  +++++  +  T   T+Q +  
Sbjct: 121 ASRLSKVITSYARRVESKPGSATAEEIGAVADCGELSELSVNYL-ETVTTELKTAQVMTA 180

Query: 137 YQADEMLSLLSAVITNEDTCYEGLVGLGVGLGLVDKVLKAISFDRKLYSLYLSLFKLGWV 196
              + + SLLS V+TN+ TC +GLV    G      +   +    +LYS+ L L     +
Sbjct: 181 ALVEHVNSLLSGVVTNQQTCLDGLVEAKSGFAAA--IGSPMGNLTRLYSISLGLVSHA-L 240

Query: 197 SDDVKAPELPKTNRFGLGNGALKLKMSPKDRAYYERLVHRK--KPAARRLLQTNNYENDG 256
           + ++K  +  K    G GN   +  +    +   +   + K  +  +R L +        
Sbjct: 241 NRNLKRFKASKGKILGGGNSTYREPLETLIKGLRKTCDNDKDCRKTSRNLGELGETSGGS 300

Query: 257 IVINGIVGVDQNGTYDFTTINAAIAAAPNNSVAAKGYYLIFVTAGIYNETVLVPKNKKYI 316
           I+++  V V    + +FTTI  AIAAAPNN+    GY++I+   G+Y E ++VP NKK +
Sbjct: 301 ILVSKAVIVGPFKSDNFTTITDAIAAAPNNTRPEDGYFVIYAREGVYEEYIVVPINKKNL 360

Query: 317 LMIGEGNNQTIITGNKNVVDGSTTFNSATVAVEGAGFFGVNLTITNTAGAAKHQAVALRV 376
           +++G+G N+TIITGN NVVDG TT+N ++ AV G  F  V++T  NTAG  KHQAVALR 
Sbjct: 361 MLMGDGINKTIITGNHNVVDGWTTYNCSSFAVVGERFMAVDVTFRNTAGPEKHQAVALRN 420

Query: 377 SADMTTFYTCIFEGYQDTLYTHSLRQFYRECDIYGTVDFIFGNAAVVFQTCNIYARLPLS 436
           +A+ ++FY C FEGYQDTLY HSLRQFYRECDIYGTVDFIFGNAA +FQ CNIYAR P++
Sbjct: 421 NAEGSSFYRCSFEGYQDTLYVHSLRQFYRECDIYGTVDFIFGNAAAIFQNCNIYARKPMA 480

Query: 437 GQFNALTAQGRTDPNQNTGTSIHNCTLKPTAELAASP-TTKSYLGRPWKQYSRTVYMQSF 496
            Q NA+TA GR DPNQNTG SI NCT+K   +LAA P +  ++LGRPWK YSRTV+MQS+
Sbjct: 481 KQKNAITAHGRLDPNQNTGISIINCTIKAAPDLAAEPKSAMTFLGRPWKPYSRTVFMQSY 540

Query: 497 IDAFIDPAGWKEWDGTLNLNTSYYAEFNNSGPGSDTSHRVQW-AVGAINATVANNFTVSN 556
           I   + P GW EW+GT+ L+T YY E++N GPG++T+ RVQW     +N   A NFTV N
Sbjct: 541 ISDIVQPVGWLEWNGTIGLDTIYYGEYSNFGPGANTNQRVQWLGYNLLNLAEAMNFTVYN 600

Query: 557 FLAGDQWLPPTTVPYTGSLIS 562
           F  GD WLP T +P+ G L+S
Sbjct: 601 FTMGDTWLPQTDIPFYGGLLS 617