Lag0007738 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0007738
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Retrotransposon protein
Location	chr9: 4017961 .. 4019636 (-)
RNA-Seq Expression	Lag0007738
Synteny	Lag0007738

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGGGGGAAGTTCTATGTCGTGTTCGTCGGGCGCATGCCTGGTGCATATATGACATGGGACGACTGTTCAAGCCAAGTAAACGGCTTTAACGGGGCGGTATATCAATCATATGACACGCTTGATGAAGCTGAGGATGCTTACAACACGTACATAGGTGGTGTAGAGGATCGTTTCATAGATCGAGGACAAGTACTCCTAGTCCGAAGCAGGCGTACACATTCTGTATGTAGGAACATAGTTAGTCCATCCCTTAGGGCCTATACTGGAACATACCACATTTTCCTCTTCGTACTAGGGTTCATACTTGGTGTAGTGGTTGTTGTACTAGTAAGTCGTTTGTAGGGAGTCAATAGGACTGATGTGTACTGGCAACTTGACTTTCAGGACTTTCGAAAATCGTACAAACCATGGATGATCATGAGATTGAAACCCAGGAGTTGATTGTAATTATGACTGTCATTTGTGCCACCCAGTACCAGTTCATAGCAGTTGTACTGGGAATCTTACAAACCAATCAGTCAACCAATTCTCGATCTCCTCCACACGTACGACATCAGATCAGGCAACCTAACTTCTTCCGCCTGATTCATGAAGATGATCTTGCATGTCGCGAAAATACACGAATGGATCGGAGAACTTTCACGATATTATGTACCTTTGTATGAACGACAGAGCGGTTGGAAGCCACGAACTACGTCGACGTGGAGGAGATGGTTGCAATGTTCCTCCACATCGTCGCACATGATGTGAAGAACCGCATAAACCGACGACAGTTTGTGCGGTCAGAAGAGACCGTGTCCAGGCATTTCAATGCTGTGTTGGATGCGATCCTCCAACGGCATACAACCCTGTTGAAAAAACCGGAGCCTGTGACGAGGACTTGCACGGACGATAGGTGGAGGTGGTTCGAGGTAATTAATTGTTGTCACATTGTCTTTGTTACGACAAGGCAACTGAACACCAATACTCGACCACTAAAACGTTATCGCTCGTGATTCACAGAACTATTTAAGTGCGTTAGATGGGACGCACATCAAGGTGAACGTGAGTGCAGCCGATCGACCCAGGTACCGAACAAGAAAGGGCGAGATCGCGACAAACGTGCTTGTCATCTGTTCGCAGAACAACGAGTTCATATTCGTCTTCCCTGGTTGGGAAGGCTCGGCCTCCGACTCAAGGGTTCTTCGCGATGCAGTGTCCCGTCCGACAGGGCTTAAGGTTCCGAGGGGATATTACTACCTGTGCGACACTGGTTATCCAAACGCTGAGGGATTTCTGGCACTCTATCGCAGCACACGGTATCACCTCACTAAGTGGCGAGGTCGGAACCCACCGACAACCCCAAAAGAGCTGTTCAACATGAGGCATTCGTCTGCGTGAAATGTAATAGAAAGAGCGTTTGGTGTTTTGAAGGGTCAGTGGGCTATTCTACGGGGGAAGTCATACTATCCTGTCGACGTACAGGTAAAAATGATAACAGCAAGTTGCCTCCTCCACAACCTGATCATTCGTGAAATGGGCCACGACTCGAGTTTCGAGACTGCACATCCAGAGACCAAGATTCAAATGGAATGAATACTGACAACATCACGTTTGTTGAGTCCACATCAGCGTGGAGTGAATGGCAGGATTCTATGGCCGAGCAGATGTTCGCGAAATGGAACAACAGTTGA

mRNA sequence

ATGGGGGGGAAGTTCTATGTCGTGTTCGTCGGGCGCATGCCTGGTGCATATATGACATGGGACGACTGTTCAAGCCAAGTAAACGGCTTTAACGGGGCGGTATATCAATCATATGACACGCTTGATGAAGCTGAGGATGCTTACAACACGTACATAGGTGGTGTAGAGGATCGTTTCATAGATCGAGGACAAGTACTCCTAGTCCGAAGCAGGCGTACACATTCTGTATGTAGGAACATAGTTAGTCCATCCCTTAGGGCCTATACTGGAACATACCACATTTTCCTCTTCGTACTAGGGTTCATACTTGGTGTAGTGGTTGTTGTACTAGTAAGTCTACCAGTTCATAGCAGTTGTACTGGGAATCTTACAAACCAATCAGTCAACCAATTCTCGATCTCCTCCACACAGCGGTTGGAAGCCACGAACTACGTCGACGTGGAGGAGATGGTTGCAATGTTCCTCCACATCGTCGCACATGATGTGAAGAACCGCATAAACCGACGACAGTTTGTGCGGTCAGAAGAGACCGTGTCCAGGCATTTCAATGCTGTGTTGGATGCGATCCTCCAACGGCATACAACCCTGTTGAAAAAACCGGAGCCTGTGACGAGGACTTGCACGGACGATAGGTGGAGGTGGTTCGAGAACTATTTAAGTGCGTTAGATGGGACGCACATCAAGGTGAACGTGAGTGCAGCCGATCGACCCAGGTACCGAACAAGAAAGGGCGAGATCGCGACAAACGTGCTTGTCATCTGTTCGCAGAACAACGAGTTCATATTCGTCTTCCCTGGTTGGGAAGGCTCGGCCTCCGACTCAAGGGTTCTTCGCGATGCAGTGTCCCGTCCGACAGGGCTTAAGGTTCCGAGGGGATATTACTACCTGTGCGACACTGGTTATCCAAACGCTGAGGGATTTCTGGCACTCTATCGCAGCACACGGTATCACCTCACTAAGTGGCGAGGTCGGAACCCACCGACAACCCCAAAAGAGCTGTTCAACATGAGGCATTCAGACCAAGATTCAAATGGAATGAATACTGACAACATCACGTTTGTTGAGTCCACATCAGCGTGGAGTGAATGGCAGGATTCTATGGCCGAGCAGATGTTCGCGAAATGGAACAACAGTTGA

Coding sequence (CDS)

ATGGGGGGGAAGTTCTATGTCGTGTTCGTCGGGCGCATGCCTGGTGCATATATGACATGGGACGACTGTTCAAGCCAAGTAAACGGCTTTAACGGGGCGGTATATCAATCATATGACACGCTTGATGAAGCTGAGGATGCTTACAACACGTACATAGGTGGTGTAGAGGATCGTTTCATAGATCGAGGACAAGTACTCCTAGTCCGAAGCAGGCGTACACATTCTGTATGTAGGAACATAGTTAGTCCATCCCTTAGGGCCTATACTGGAACATACCACATTTTCCTCTTCGTACTAGGGTTCATACTTGGTGTAGTGGTTGTTGTACTAGTAAGTCTACCAGTTCATAGCAGTTGTACTGGGAATCTTACAAACCAATCAGTCAACCAATTCTCGATCTCCTCCACACAGCGGTTGGAAGCCACGAACTACGTCGACGTGGAGGAGATGGTTGCAATGTTCCTCCACATCGTCGCACATGATGTGAAGAACCGCATAAACCGACGACAGTTTGTGCGGTCAGAAGAGACCGTGTCCAGGCATTTCAATGCTGTGTTGGATGCGATCCTCCAACGGCATACAACCCTGTTGAAAAAACCGGAGCCTGTGACGAGGACTTGCACGGACGATAGGTGGAGGTGGTTCGAGAACTATTTAAGTGCGTTAGATGGGACGCACATCAAGGTGAACGTGAGTGCAGCCGATCGACCCAGGTACCGAACAAGAAAGGGCGAGATCGCGACAAACGTGCTTGTCATCTGTTCGCAGAACAACGAGTTCATATTCGTCTTCCCTGGTTGGGAAGGCTCGGCCTCCGACTCAAGGGTTCTTCGCGATGCAGTGTCCCGTCCGACAGGGCTTAAGGTTCCGAGGGGATATTACTACCTGTGCGACACTGGTTATCCAAACGCTGAGGGATTTCTGGCACTCTATCGCAGCACACGGTATCACCTCACTAAGTGGCGAGGTCGGAACCCACCGACAACCCCAAAAGAGCTGTTCAACATGAGGCATTCAGACCAAGATTCAAATGGAATGAATACTGACAACATCACGTTTGTTGAGTCCACATCAGCGTGGAGTGAATGGCAGGATTCTATGGCCGAGCAGATGTTCGCGAAATGGAACAACAGTTGA

Protein sequence

MGGKFYVVFVGRMPGAYMTWDDCSSQVNGFNGAVYQSYDTLDEAEDAYNTYIGGVEDRFIDRGQVLLVRSRRTHSVCRNIVSPSLRAYTGTYHIFLFVLGFILGVVVVVLVSLPVHSSCTGNLTNQSVNQFSISSTQRLEATNYVDVEEMVAMFLHIVAHDVKNRINRRQFVRSEETVSRHFNAVLDAILQRHTTLLKKPEPVTRTCTDDRWRWFENYLSALDGTHIKVNVSAADRPRYRTRKGEIATNVLVICSQNNEFIFVFPGWEGSASDSRVLRDAVSRPTGLKVPRGYYYLCDTGYPNAEGFLALYRSTRYHLTKWRGRNPPTTPKELFNMRHSDQDSNGMNTDNITFVESTSAWSEWQDSMAEQMFAKWNNS

Homology

[Close]

BLAST of Lag0007738 vs. NCBI nr
Match: KAA0065306.1 (retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 346.7 bits (888), Expect = 2.6e-91
Identity = 174/300 (58.00%), Postives = 200/300 (66.67%), Query Frame = 0


Query: 139 LEATNYVDVEEMVAMFLHIVAHDVKNRINRRQFVRSEETVSRHFNAVLDAILQRHTTLLK 198
           LEAT YVDVEEM+A+FLHIVAHDVKNR+ RR F RS ETVSRHFNAV    L+ H  LLK
Sbjct: 19  LEATQYVDVEEMIAIFLHIVAHDVKNRVTRRHFARSGETVSRHFNAV----LRLHEILLK 78

Query: 199 KPEPVTRTCTDDRWRWFENYLSALDGTHIKVNVSAADRPRYRTRKGEIATNVLVICSQNN 258
           +P+PVT +C+ ++WRWF+  L ALDGTHIKVNVS +DRPRYR+RKG+I TNVL +C QN 
Sbjct: 79  QPDPVTYSCSHEKWRWFQKCLGALDGTHIKVNVSMSDRPRYRSRKGDITTNVLGVCLQNG 138

Query: 259 EFIFVFPGWEGSASDSRVLRDAVSRPTGLKVPRGYYYLCDTGYPNAEGFLALYRSTRYHL 318
           EFIFV PGWEGSASDSRVLRDAVSR TGLKVP+GYYYLCD GYPNAEGFLA YR  RYHL
Sbjct: 139 EFIFVMPGWEGSASDSRVLRDAVSRLTGLKVPKGYYYLCDAGYPNAEGFLAPYRGQRYHL 198

Query: 319 TKWRGRNPPTTPKELFNMRHS--------------------------------------- 378
           T+WRG NPP  PKELFNMRHS                                       
Sbjct: 199 TEWRGGNPPKCPKELFNMRHSFARNVIERAFGSLNCRWTILQGRSYYLVDIQCKIITACC 258

[Close]

BLAST of Lag0007738 vs. NCBI nr
Match: KAA0048191.1 (retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 332.0 bits (850), Expect = 6.6e-87
Identity = 167/309 (54.05%), Postives = 196/309 (63.43%), Query Frame = 0


Query: 139 LEATNYVDVEEMVAMFLHIVAHDVKNRINRRQFVRSEETVSRHFNAVLDAILQRHTTLLK 198
           LEAT YVDVEEM A+FLHIVAHDVKNR+ RR F RS+ TVSRHFN VL+A+L+ H  LLK
Sbjct: 19  LEATQYVDVEEMTAIFLHIVAHDVKNRVARRHFARSDATVSRHFNVVLNAVLRIHEILLK 78

Query: 199 KPEPVTRTCTDDRWRWFENY---------LSALDGTHIKVNVSAADRPRYRTRKGEIATN 258
           +P+ VT +C+ ++WRWF+ Y         + ALDGTHIKVNVS +D PRYR+RK +I TN
Sbjct: 79  QPDQVTHSCSHEKWRWFQVYNWPATTLQSICALDGTHIKVNVSMSDHPRYRSRKSDITTN 138

Query: 259 VLVICSQNNEFIFVFPGWEGSASDSRVLRDAVSRPTGLKVPRGYYYLCDTGYPNAEGFLA 318
           VL +CSQN EFIFV PGWEGSASDSRVLRD VSRP GLKVP+GYYYLCD  Y N EGFLA
Sbjct: 139 VLGLCSQNGEFIFVMPGWEGSASDSRVLRDVVSRPIGLKVPKGYYYLCDASYLNVEGFLA 198

Query: 319 LYRSTRYHLTKWRGRNPPTTPKELFNMRHS------------------------------ 378
            YR  RYHL +WRG NPP  PKELFNMRHS                              
Sbjct: 199 PYRGQRYHLIEWRGGNPPKCPKELFNMRHSSARNVIERAFGSLKSRWAILRGRSYYPVDI 258

[Close]

BLAST of Lag0007738 vs. NCBI nr
Match: XP_008455792.1 (PREDICTED: putative nuclease HARBI1 [Cucumis melo])

HSP 1 Score: 314.7 bits (805), Expect = 1.1e-81
Identity = 156/289 (53.98%), Postives = 190/289 (65.74%), Query Frame = 0


Query: 133 ISSTQRLEATNYVDVEEMVAMFLHIVAHDVKNRINRRQFVRSEETVSRHFNAVLDAILQR 192
           + +T  L  T  +DVEEMVAMFLHI+AHDVKNR+ +R+FVRS ETVSRHFN VL A  + 
Sbjct: 13  LRTTAGLVETEVIDVEEMVAMFLHILAHDVKNRMIQREFVRSGETVSRHFNIVLLAGFRL 72

Query: 193 HTTLLKKPEPVTRTCTDDRWRWFENYLSALDGTHIKVNVSAADRPRYRTRKGEIATNVLV 252
           H  LLKKP+PVT +CTD RW+WFEN L ALDGT+IKVNVSA DRPRYRTRKGE+ATNVL 
Sbjct: 73  HDELLKKPQPVTNSCTDPRWKWFENCLGALDGTYIKVNVSATDRPRYRTRKGEVATNVLG 132

Query: 253 ICSQNNEFIFVFPGWEGSASDSRVLRDAVSRPTGLKVPRGYYYLCDTGYPNAEGFLALYR 312
            C    +F+FV  GWEGSA+DSR+LRDA+SR  GLKVP+GYYYLCD GYPNAEGFLA YR
Sbjct: 133 ACDTKGDFVFVLFGWEGSAADSRILRDAISRHNGLKVPKGYYYLCDAGYPNAEGFLAPYR 192

Query: 313 STRYHLTKWRGR-NPPTTPKELFNMRHS-------------------------------- 372
             RYHL++WRG  N PTT +E FNM+HS                                
Sbjct: 193 GERYHLSEWRGESNAPTTAREFFNMKHSSARNVIERAFGLLKGRWAILRGKSYYPVDVQC 252

Query: 373 -------------DQDSNGMNTDNITFVESTSAWSEWQDSMAEQMFAKW 376
                        +++        I ++E+++ WSEW+D +A  MF+ W
Sbjct: 253 RTIMACCLLHNLINREMTNSEIIEINYIEASNEWSEWRDQLAHTMFSDW 301

[Close]

BLAST of Lag0007738 vs. NCBI nr
Match: KAA0055632.1 (retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 313.9 bits (803), Expect = 1.9e-81
Identity = 146/243 (60.08%), Postives = 183/243 (75.31%), Query Frame = 0


Query: 139 LEATNYVDVEEMVAMFLHIVAHDVKNRINRRQFVRSEETVSRHFNAVLDAILQRHTTLLK 198
           L +T  VDVEEMVAMFLH++ HDVKNR+ +R+FVRS ETVSRHFN +L A+L+ +  L+K
Sbjct: 19  LSSTKIVDVEEMVAMFLHVLVHDVKNRVIQREFVRSGETVSRHFNIILLAVLRLYEELIK 78

Query: 199 KPEPVTRTCTDDRWRWFENYLSALDGTHIKVNVSAADRPRYRTRKGEIATNVLVICSQNN 258
           +P PV   C D RW+ FEN L ALDGT+IKVNV A DRP +RTRKGEIATNVL IC    
Sbjct: 79  RPVPVISNCNDQRWKCFENCLGALDGTYIKVNVPAGDRPTFRTRKGEIATNVLGICDTKG 138

Query: 259 EFIFVFPGWEGSASDSRVLRDAVSRPTGLKVPRGYYYLCDTGYPNAEGFLALYRSTRYHL 318
           +F++V  GWEGSA+DSR+LRDA+SR  GL+VP GYYYLCD GYPNAEGFL  YR  RYHL
Sbjct: 139 DFVYVLAGWEGSAADSRILRDAISRENGLEVPNGYYYLCDAGYPNAEGFLTPYRGQRYHL 198

Query: 319 TKWRG-RNPPTTPKELFNMRHSD-----QDSNGMNTDNITFVESTSAWSEWQDSMAEQMF 376
            +WRG  N PT  KE FNM+HS      + + G+  ++I ++E+T+ WS+W+D +AE MF
Sbjct: 199 QEWRGTANAPTNAKEYFNMKHSSVRNVIERAFGVLKEDIQYIETTNEWSQWRDDLAESMF 258

[Close]

BLAST of Lag0007738 vs. NCBI nr
Match: TYK08067.1 (retrotransposon protein [Cucumis melo var. makuwa])

HSP 1 Score: 313.2 bits (801), Expect = 3.2e-81
Identity = 162/300 (54.00%), Postives = 186/300 (62.00%), Query Frame = 0


Query: 139 LEATNYVDVEEMVAMFLHIVAHDVKNRINRRQFVRSEETVSRHFNAVLDAILQRHTTLLK 198
           LEAT YVDVEEM A+FLHIVAHDVKNR+ RR F RS+ TVSRHFN VL+A+L+ H  LLK
Sbjct: 19  LEATQYVDVEEMTAIFLHIVAHDVKNRVARRHFARSDATVSRHFNVVLNAVLRIHEILLK 78

Query: 199 KPEPVTRTCTDDRWRWFENYLSALDGTHIKVNVSAADRPRYRTRKGEIATNVLVICSQNN 258
           +P+ VT +C             ALDGTHIKVNVS +D PRYR+RK +I TNVL +CSQN 
Sbjct: 79  QPDQVTHSC-------------ALDGTHIKVNVSMSDHPRYRSRKSDITTNVLGLCSQNG 138

Query: 259 EFIFVFPGWEGSASDSRVLRDAVSRPTGLKVPRGYYYLCDTGYPNAEGFLALYRSTRYHL 318
           EFIFV PGWEGSASDSRVLRD VSRP GLKVP+GYYYLCD  Y N EGFLA YR  RYHL
Sbjct: 139 EFIFVMPGWEGSASDSRVLRDVVSRPIGLKVPKGYYYLCDASYLNVEGFLAPYRGQRYHL 198

Query: 319 TKWRGRNPPTTPKELFNMRHS--------------------------------------- 378
            +WRG NPP  PKELFNMRHS                                       
Sbjct: 199 IEWRGGNPPKCPKELFNMRHSSARNVIERAFGSLKSRWAILRGRSYYPVDIQCKIITACC 258

[Close]

BLAST of Lag0007738 vs. ExPASy Swiss-Prot
Match: Q07762 (Ribonuclease H OS=Crithidia fasciculata OX=5656 GN=RNH1 PE=3 SV=1)

HSP 1 Score: 61.6 bits (148), Expect = 2.2e-08
Identity = 31/50 (62.00%), Postives = 34/50 (68.00%), Query Frame = 0


Query: 1   MGGKFYVVFVGRMPGAYMTWDDCSSQVNGFNGAVYQSYDTLDEAEDAYNT 51
           M   FYVV VGR  G Y TWD CS QV GF+GAVY+S+ TL EA  AY T
Sbjct: 152 MKPSFYVVAVGRQRGIYSTWDQCSEQVKGFSGAVYKSFRTLSEAR-AYLT 200

[Close]

BLAST of Lag0007738 vs. ExPASy Swiss-Prot
Match: Q9KEI9 (Ribonuclease H OS=Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 7344 / JCM 9153 / C-125) OX=272558 GN=rnhA PE=1 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 2.7e-06
Identity = 22/45 (48.89%), Postives = 32/45 (71.11%), Query Frame = 0


Query: 4  KFYVVFVGRMPGAYMTWDDCSSQVNGFNGAVYQSYDTLDEAEDAY 49
          K+YVV+ GR PG Y +W  C +QV G+ GA ++SY + +EAE A+
Sbjct: 5  KYYVVWNGRKPGIYTSWSACEAQVKGYTGAKFKSYPSKEEAEAAF 49

[Close]

BLAST of Lag0007738 vs. ExPASy Swiss-Prot
Match: Q04740 (Ribonuclease H OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=RNH1 PE=1 SV=2)

HSP 1 Score: 50.4 bits (119), Expect = 5.0e-05
Identity = 18/43 (41.86%), Postives = 31/43 (72.09%), Query Frame = 0


Query: 3  GKFYVVFVGRMPGAYMTWDDCSSQVNGFNGAVYQSYDTLDEAE 46
          G FY V  GR  G Y TW++C +QV+G+ GA+Y+ +++ ++A+
Sbjct: 5  GNFYAVRKGRETGIYNTWNECKNQVDGYGGAIYKKFNSYEQAK 47

[Close]

BLAST of Lag0007738 vs. ExPASy TrEMBL
Match: A0A5A7VG45 (Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold1023G00220 PE=3 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 1.3e-91
Identity = 174/300 (58.00%), Postives = 200/300 (66.67%), Query Frame = 0


Query: 139 LEATNYVDVEEMVAMFLHIVAHDVKNRINRRQFVRSEETVSRHFNAVLDAILQRHTTLLK 198
           LEAT YVDVEEM+A+FLHIVAHDVKNR+ RR F RS ETVSRHFNAV    L+ H  LLK
Sbjct: 19  LEATQYVDVEEMIAIFLHIVAHDVKNRVTRRHFARSGETVSRHFNAV----LRLHEILLK 78

Query: 199 KPEPVTRTCTDDRWRWFENYLSALDGTHIKVNVSAADRPRYRTRKGEIATNVLVICSQNN 258
           +P+PVT +C+ ++WRWF+  L ALDGTHIKVNVS +DRPRYR+RKG+I TNVL +C QN 
Sbjct: 79  QPDPVTYSCSHEKWRWFQKCLGALDGTHIKVNVSMSDRPRYRSRKGDITTNVLGVCLQNG 138

Query: 259 EFIFVFPGWEGSASDSRVLRDAVSRPTGLKVPRGYYYLCDTGYPNAEGFLALYRSTRYHL 318
           EFIFV PGWEGSASDSRVLRDAVSR TGLKVP+GYYYLCD GYPNAEGFLA YR  RYHL
Sbjct: 139 EFIFVMPGWEGSASDSRVLRDAVSRLTGLKVPKGYYYLCDAGYPNAEGFLAPYRGQRYHL 198

Query: 319 TKWRGRNPPTTPKELFNMRHS--------------------------------------- 378
           T+WRG NPP  PKELFNMRHS                                       
Sbjct: 199 TEWRGGNPPKCPKELFNMRHSFARNVIERAFGSLNCRWTILQGRSYYLVDIQCKIITACC 258

[Close]

BLAST of Lag0007738 vs. ExPASy TrEMBL
Match: A0A5A7U3R2 (Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold63G001010 PE=3 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 3.2e-87
Identity = 167/309 (54.05%), Postives = 196/309 (63.43%), Query Frame = 0


Query: 139 LEATNYVDVEEMVAMFLHIVAHDVKNRINRRQFVRSEETVSRHFNAVLDAILQRHTTLLK 198
           LEAT YVDVEEM A+FLHIVAHDVKNR+ RR F RS+ TVSRHFN VL+A+L+ H  LLK
Sbjct: 19  LEATQYVDVEEMTAIFLHIVAHDVKNRVARRHFARSDATVSRHFNVVLNAVLRIHEILLK 78

Query: 199 KPEPVTRTCTDDRWRWFENY---------LSALDGTHIKVNVSAADRPRYRTRKGEIATN 258
           +P+ VT +C+ ++WRWF+ Y         + ALDGTHIKVNVS +D PRYR+RK +I TN
Sbjct: 79  QPDQVTHSCSHEKWRWFQVYNWPATTLQSICALDGTHIKVNVSMSDHPRYRSRKSDITTN 138

Query: 259 VLVICSQNNEFIFVFPGWEGSASDSRVLRDAVSRPTGLKVPRGYYYLCDTGYPNAEGFLA 318
           VL +CSQN EFIFV PGWEGSASDSRVLRD VSRP GLKVP+GYYYLCD  Y N EGFLA
Sbjct: 139 VLGLCSQNGEFIFVMPGWEGSASDSRVLRDVVSRPIGLKVPKGYYYLCDASYLNVEGFLA 198

Query: 319 LYRSTRYHLTKWRGRNPPTTPKELFNMRHS------------------------------ 378
            YR  RYHL +WRG NPP  PKELFNMRHS                              
Sbjct: 199 PYRGQRYHLIEWRGGNPPKCPKELFNMRHSSARNVIERAFGSLKSRWAILRGRSYYPVDI 258

[Close]

BLAST of Lag0007738 vs. ExPASy TrEMBL
Match: A0A1S3C1U8 (putative nuclease HARBI1 OS=Cucumis melo OX=3656 GN=LOC103495887 PE=3 SV=1)

HSP 1 Score: 314.7 bits (805), Expect = 5.3e-82
Identity = 156/289 (53.98%), Postives = 190/289 (65.74%), Query Frame = 0


Query: 133 ISSTQRLEATNYVDVEEMVAMFLHIVAHDVKNRINRRQFVRSEETVSRHFNAVLDAILQR 192
           + +T  L  T  +DVEEMVAMFLHI+AHDVKNR+ +R+FVRS ETVSRHFN VL A  + 
Sbjct: 13  LRTTAGLVETEVIDVEEMVAMFLHILAHDVKNRMIQREFVRSGETVSRHFNIVLLAGFRL 72

Query: 193 HTTLLKKPEPVTRTCTDDRWRWFENYLSALDGTHIKVNVSAADRPRYRTRKGEIATNVLV 252
           H  LLKKP+PVT +CTD RW+WFEN L ALDGT+IKVNVSA DRPRYRTRKGE+ATNVL 
Sbjct: 73  HDELLKKPQPVTNSCTDPRWKWFENCLGALDGTYIKVNVSATDRPRYRTRKGEVATNVLG 132

Query: 253 ICSQNNEFIFVFPGWEGSASDSRVLRDAVSRPTGLKVPRGYYYLCDTGYPNAEGFLALYR 312
            C    +F+FV  GWEGSA+DSR+LRDA+SR  GLKVP+GYYYLCD GYPNAEGFLA YR
Sbjct: 133 ACDTKGDFVFVLFGWEGSAADSRILRDAISRHNGLKVPKGYYYLCDAGYPNAEGFLAPYR 192

Query: 313 STRYHLTKWRGR-NPPTTPKELFNMRHS-------------------------------- 372
             RYHL++WRG  N PTT +E FNM+HS                                
Sbjct: 193 GERYHLSEWRGESNAPTTAREFFNMKHSSARNVIERAFGLLKGRWAILRGKSYYPVDVQC 252

Query: 373 -------------DQDSNGMNTDNITFVESTSAWSEWQDSMAEQMFAKW 376
                        +++        I ++E+++ WSEW+D +A  MF+ W
Sbjct: 253 RTIMACCLLHNLINREMTNSEIIEINYIEASNEWSEWRDQLAHTMFSDW 301

[Close]

BLAST of Lag0007738 vs. ExPASy TrEMBL
Match: A0A5A7UIG4 (Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold222G001240 PE=3 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 9.0e-82
Identity = 146/243 (60.08%), Postives = 183/243 (75.31%), Query Frame = 0


Query: 139 LEATNYVDVEEMVAMFLHIVAHDVKNRINRRQFVRSEETVSRHFNAVLDAILQRHTTLLK 198
           L +T  VDVEEMVAMFLH++ HDVKNR+ +R+FVRS ETVSRHFN +L A+L+ +  L+K
Sbjct: 19  LSSTKIVDVEEMVAMFLHVLVHDVKNRVIQREFVRSGETVSRHFNIILLAVLRLYEELIK 78

Query: 199 KPEPVTRTCTDDRWRWFENYLSALDGTHIKVNVSAADRPRYRTRKGEIATNVLVICSQNN 258
           +P PV   C D RW+ FEN L ALDGT+IKVNV A DRP +RTRKGEIATNVL IC    
Sbjct: 79  RPVPVISNCNDQRWKCFENCLGALDGTYIKVNVPAGDRPTFRTRKGEIATNVLGICDTKG 138

Query: 259 EFIFVFPGWEGSASDSRVLRDAVSRPTGLKVPRGYYYLCDTGYPNAEGFLALYRSTRYHL 318
           +F++V  GWEGSA+DSR+LRDA+SR  GL+VP GYYYLCD GYPNAEGFL  YR  RYHL
Sbjct: 139 DFVYVLAGWEGSAADSRILRDAISRENGLEVPNGYYYLCDAGYPNAEGFLTPYRGQRYHL 198

Query: 319 TKWRG-RNPPTTPKELFNMRHSD-----QDSNGMNTDNITFVESTSAWSEWQDSMAEQMF 376
            +WRG  N PT  KE FNM+HS      + + G+  ++I ++E+T+ WS+W+D +AE MF
Sbjct: 199 QEWRGTANAPTNAKEYFNMKHSSVRNVIERAFGVLKEDIQYIETTNEWSQWRDDLAESMF 258

[Close]

BLAST of Lag0007738 vs. ExPASy TrEMBL
Match: A0A5D3C7X6 (Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold265G002160 PE=3 SV=1)

HSP 1 Score: 313.2 bits (801), Expect = 1.5e-81
Identity = 162/300 (54.00%), Postives = 186/300 (62.00%), Query Frame = 0


Query: 139 LEATNYVDVEEMVAMFLHIVAHDVKNRINRRQFVRSEETVSRHFNAVLDAILQRHTTLLK 198
           LEAT YVDVEEM A+FLHIVAHDVKNR+ RR F RS+ TVSRHFN VL+A+L+ H  LLK
Sbjct: 19  LEATQYVDVEEMTAIFLHIVAHDVKNRVARRHFARSDATVSRHFNVVLNAVLRIHEILLK 78

Query: 199 KPEPVTRTCTDDRWRWFENYLSALDGTHIKVNVSAADRPRYRTRKGEIATNVLVICSQNN 258
           +P+ VT +C             ALDGTHIKVNVS +D PRYR+RK +I TNVL +CSQN 
Sbjct: 79  QPDQVTHSC-------------ALDGTHIKVNVSMSDHPRYRSRKSDITTNVLGLCSQNG 138

Query: 259 EFIFVFPGWEGSASDSRVLRDAVSRPTGLKVPRGYYYLCDTGYPNAEGFLALYRSTRYHL 318
           EFIFV PGWEGSASDSRVLRD VSRP GLKVP+GYYYLCD  Y N EGFLA YR  RYHL
Sbjct: 139 EFIFVMPGWEGSASDSRVLRDVVSRPIGLKVPKGYYYLCDASYLNVEGFLAPYRGQRYHL 198

Query: 319 TKWRGRNPPTTPKELFNMRHS--------------------------------------- 378
            +WRG NPP  PKELFNMRHS                                       
Sbjct: 199 IEWRGGNPPKCPKELFNMRHSSARNVIERAFGSLKSRWAILRGRSYYPVDIQCKIITACC 258

[Close]

BLAST of Lag0007738 vs. TAIR 10
Match: AT5G41980.1 (CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (InterPro:IPR006912); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G43722.1); Has 1807 Blast hits to 1807 proteins in 277 species: Archae - 0; Bacteria - 0; Metazoa - 736; Fungi - 347; Plants - 385; Viruses - 0; Other Eukaryotes - 339 (source: NCBI BLink). )

HSP 1 Score: 128.3 bits (321), Expect = 1.3e-29
Identity = 72/200 (36.00%), Postives = 107/200 (53.50%), Query Frame = 0


Query: 139 LEATNYVDVEEMVAMFLHIVAHDVKNRINRRQFVRSEETVSRHFNAVLDAILQRHTTLLK 198
           L  TN + +E  +A+FL I+ H+++ R  +  F  S ET+SRHFN VL+A++        
Sbjct: 66  LRHTNRIKIEAQLAIFLFIIGHNLRTRAVQELFCYSGETISRHFNNVLNAVIAISKDFF- 125

Query: 199 KPEPVTRTCTDDRWRWFENYLSALDGTHIKVNVSAADRPRYRTRKGEIATNVLVICSQNN 258
           +P   + T  +D   +F++ +  +D  HI V V   ++  +R   G +  NVL   S + 
Sbjct: 126 QPNSNSDTLENDD-PYFKDCVGVVDSFHIPVMVGVDEQGPFRNGNGLLTQNVLAASSFDL 185

Query: 259 EFIFVFPGWEGSASDSRVLRDAVSRPTGLKVPRGYYYLCDTGYPNAEGFLALYRSTRYHL 318
            F +V  GWEGSASD +VL  A++R   L+VP+G YY+ D  YPN  GF+A Y       
Sbjct: 186 RFNYVLAGWEGSASDQQVLNAALTRRNKLQVPQGKYYIVDNKYPNLPGFIAPYHGV---- 245

Query: 319 TKWRGRNPPTTPKELFNMRH 339
                 N     KE+FN RH
Sbjct: 246 ----STNSREEAKEMFNERH 255

[Close]

BLAST of Lag0007738 vs. TAIR 10
Match: AT1G43722.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G28730.1); Has 924 Blast hits to 912 proteins in 109 species: Archae - 0; Bacteria - 0; Metazoa - 222; Fungi - 31; Plants - 661; Viruses - 0; Other Eukaryotes - 10 (source: NCBI BLink). )

HSP 1 Score: 112.5 bits (280), Expect = 7.6e-25
Identity = 72/214 (33.64%), Postives = 105/214 (49.07%), Query Frame = 0


Query: 139 LEATNYVDVEEMVAMFLHIVAHDVKNRINRRQFVRSEETVSRHFNAVLDAILQRHTTLLK 198
           L+ T  + +EE VAMFL I  H+   R    +F R++ETV R F  VL A        ++
Sbjct: 90  LQPTLNISIEESVAMFLRICGHNEVYRDVGLRFGRNQETVQRKFREVLTATELLACDYIR 149

Query: 199 KP------EPVTRTCTDDR-WRWFENYLSALDGTHIKVNVSAADRPRYRTRKGEIATNVL 258
            P          R   D R W +F  ++ A+DGTH+ V V    +  Y  R    + N++
Sbjct: 150 TPTRQELYRIPERLQVDQRYWPYFSGFVGAMDGTHVCVKVKPDLQGMYWNRHDNASLNIM 209

Query: 259 VICSQNNEFIFVFPGWEGSASDSRVLRDAVSRPTGLKVPRG-YYYLCDTGYPNAEGFLAL 318
            IC     F +++ G  GS  D+ VL+ A    +   +P    YYL D+GYPN +G LA 
Sbjct: 210 AICDLKMLFTYIWNGAPGSCYDTAVLQIAQQSDSEFPLPPSEKYYLVDSGYPNKQGLLAP 269

Query: 319 YRST-----RYHLTKWRGRNPPTTPKELFNMRHS 340
           YRS+     RYH++++     P    ELFN  H+
Sbjct: 270 YRSSRNRVVRYHMSQFYYGPRPRNKHELFNQCHT 303

[Close]

BLAST of Lag0007738 vs. TAIR 10
Match: AT5G35695.1 (CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (InterPro:IPR006912); BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G41980.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 78.2 bits (191), Expect = 1.6e-14
Identity = 40/80 (50.00%), Postives = 49/80 (61.25%), Query Frame = 0


Query: 260 FIFVFPGWEGSASDSRVLRDAVSRPTGLKVPRGYYYLCDTGYPNAEGFLALYRSTRYHLT 319
           FI+V  GWEGSA DSRVL DA+ +          +YL D G+ N   FLA +R  RYHL 
Sbjct: 25  FIYVLSGWEGSAHDSRVLSDALRK----------FYLVDCGFANRLNFLAPFRGVRYHLQ 84

Query: 320 KWRG-RNPPTTPKELFNMRH 339
           ++ G R  P TP ELFN+RH
Sbjct: 85  EFAGQRRDPETPHELFNLRH 94

[Close]

BLAST of Lag0007738 vs. TAIR 10
Match: AT5G28950.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G41980.1); Has 448 Blast hits to 446 proteins in 74 species: Archae - 0; Bacteria - 0; Metazoa - 31; Fungi - 21; Plants - 396; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 78.2 bits (191), Expect = 1.6e-14
Identity = 36/78 (46.15%), Postives = 53/78 (67.95%), Query Frame = 0


Query: 214 WFENYLSALDGTHIKVNVSAADRPRYRTRKGEIATNVLVICSQNNEFIFVFPGWEGSASD 273
           +F++ + A+D THI   VS    P +R RKG+I+ N+L  C+ + EF++V  GWEGSA D
Sbjct: 21  YFKDCVGAIDDTHIFAMVSQKKMPSFRNRKGDISQNMLAACNFDVEFMYVLSGWEGSAHD 80

Query: 274 SRVLRDAVSRPTG-LKVP 291
           S+VL DA++R +  L VP
Sbjct: 81  SKVLNDALTRNSNRLPVP 98

[Close]

BLAST of Lag0007738 vs. TAIR 10
Match: AT5G28730.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G43722.1); Has 496 Blast hits to 496 proteins in 68 species: Archae - 0; Bacteria - 0; Metazoa - 3; Fungi - 23; Plants - 470; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 69.3 bits (168), Expect = 7.4e-12
Identity = 57/175 (32.57%), Postives = 81/175 (46.29%), Query Frame = 0


Query: 139 LEATNYVDVEEMVAMFLHIVAHDVKNRINRRQFVRSEETVSRHFNAVLDAILQRHTTLLK 198
           L+++  + ++E VA+FL I A +   R    +F  ++ET+ R F+ VL A ++R      
Sbjct: 48  LQSSTNISLDESVAIFLIICASNDTQRDIALRFGHAQETIWRKFHDVLKA-MERLAVEYI 107

Query: 199 KPEPVTRTCTDDRWRWFENYLSALDGTHIKVNVSAADRPRYRTRKGEIATNVLVICSQNN 258
           +P  V      +  R   N L   D T           P      G  + NVL IC  + 
Sbjct: 108 RPRKV------EELRAISNRLQ--DDTRY--------WPFLMDLLGIASFNVLAICDLDM 167

Query: 259 EFIFVFPGWEGSASDSRVLRDAVSRPTGLKV-PRGYYYLCDTGYPNAEGFLALYR 313
            F + F G  GS  D+RVL  A+S      V P   YYL D+GY N  G+LA YR
Sbjct: 168 LFTYCFVGMAGSTHDARVLSAAISDDPLFHVPPDSKYYLVDSGYANKRGYLAPYR 205

The following BLAST results are available for this feature:

BLAST of Lag0007738 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0065306.1	2.6e-91	58.00	retrotransposon protein [Cucumis melo var. makuwa]	[more]
KAA0048191.1	6.6e-87	54.05	retrotransposon protein [Cucumis melo var. makuwa]	[more]
XP_008455792.1	1.1e-81	53.98	PREDICTED: putative nuclease HARBI1 [Cucumis melo]	[more]
KAA0055632.1	1.9e-81	60.08	retrotransposon protein [Cucumis melo var. makuwa]	[more]
TYK08067.1	3.2e-81	54.00	retrotransposon protein [Cucumis melo var. makuwa]	[more]

BLAST of Lag0007738 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q07762	2.2e-08	62.00	Ribonuclease H OS=Crithidia fasciculata OX=5656 GN=RNH1 PE=3 SV=1	[more]
Q9KEI9	2.7e-06	48.89	Ribonuclease H OS=Bacillus halodurans (strain ATCC BAA-125 / DSM 18197 / FERM 73...	[more]
Q04740	5.0e-05	41.86	Ribonuclease H OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=55929...	[more]

BLAST of Lag0007738 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7VG45	1.3e-91	58.00	Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold...	[more]
A0A5A7U3R2	3.2e-87	54.05	Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold...	[more]
A0A1S3C1U8	5.3e-82	53.98	putative nuclease HARBI1 OS=Cucumis melo OX=3656 GN=LOC103495887 PE=3 SV=1	[more]
A0A5A7UIG4	9.0e-82	60.08	Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold...	[more]
A0A5D3C7X6	1.5e-81	54.00	Retrotransposon protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]

BLAST of Lag0007738 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G41980.1	1.3e-29	36.00	CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (Int...	[more]
AT1G43722.1	7.6e-25	33.64	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT5G35695.1	1.6e-14	50.00	CONTAINS InterPro DOMAIN/s: Putative harbinger transposase-derived nuclease (Int...	[more]
AT5G28950.1	1.6e-14	46.15	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT5G28730.1	7.4e-12	32.57	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01

Position :

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x 1


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR027806	Harbinger transposase-derived nuclease domain	PFAM	PF13359	DDE_Tnp_4	coord: 222..339 e-value: 3.5E-13 score: 49.5
IPR037056	Ribonuclease H1, N-terminal domain superfamily	GENE3D	3.40.970.10		coord: 3..47 e-value: 2.7E-16 score: 61.7
IPR011320	Ribonuclease H1, N-terminal	PFAM	PF01693	Cauli_VI	coord: 4..46 e-value: 3.0E-16 score: 59.4
None	No IPR available	PANTHER	PTHR22930:SF201	NUCLEASE HARBI1-LIKE PROTEIN	coord: 141..339
None	No IPR available	PANTHER	PTHR22930	UNCHARACTERIZED	coord: 141..339
IPR009027	Ribosomal protein L9/RNase H1, N-terminal	SUPERFAMILY	55658	L9 N-domain-like	coord: 4..48

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0007738.1	Lag0007738.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046872	metal ion binding