Lag0007032 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0007032
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Description1-aminocyclopropane-1-carboxylate oxidase 1
Locationchr6: 48223137 .. 48225359 (+)
RNA-Seq ExpressionLag0007032
SyntenyLag0007032
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGATTCCTACCATTGATTTCAATCAGCTCACTGGAGACAACAGGACCAAAACCCTGTCTGTCCTTCATGATGCTTGCAAAGATTGGGGCTTCTTTTTGGTACATTATTTCTCTTTTCTCTTTACAATTCTGATCGGTATTCGTTAAAATATCCCGTCATCTTTACATCTCTTTCGGGTAATATTTTTATATTTGTTTCGTCTAAAAAACCTCGAGTTAATGAAGATGTTATCTTTTTTACTCATATCTAACCACTTTCACCACTCATGTGAGGTTGTGTTTACGTTCTCAACCATCTTTGTCTGTCTCGTATAAAAGGAGACTAGTTGGGAAGTCTCCCCTTAAATAGTGATATCTGATCTATTTGTTCTTTTCCTAAGTGTATCGAGAGTTTGATCTTATCCAATCAAATTTTCTTACTTGAAGTATGGCCACCACTAGATTACTTTAAAACTCGAGTCAATAGATTCAAGTATATTTAATATTTTCTAATGAAAACCCAATGACTAAAAAGGGATCGGGAGAAATCCAACTCAAGTAGGTTTATTTATTTACTTTTTTGAGTTCAACACCATGTGGAGGTGAGAGGATCTATGTCTTTGATCTCCTAGTCAGTGGTACATATCTTAACCAGTTGAACTATGCTCGTTTTTTTTTATTTGGGTGTAGTTAAATGGAATTGTTCATGGTTAAGTTTGGTTTTTATGGGTTCTTTATTTTCTTTTCTTGGGAAATGTTAATGGTTTGTTTGAGTGGTATTGGTGTGCAGGTAGAGAACCATGGGATTGAAGAGGAAGTATTGGAGAAAGTGAAGCTGTTGGTAAACAGACATTATGATGAGAATATGAAAGAAAAATTTTATAATTCAGATGTGGCCAAAAACTTGGGCAAAATGGCAGTATCTGATGTGGATTGGGAAAGCACTTTCTTCATTTGGCACAAACCAAACTCTAACATCTCTCATTTTACCAATGTCTCACAAGATCTTTGGTAAGTTTCTCTATTTAAGTATTTAGGTCACCTTTTGAGTTTTGACAATATTTTTAATTCTTTGCTTTTATTTCATAATGGTGTACCACATCAGCACAACAAATTTTAAATTTTAAAATAAAAGTTAAGAGGGTAACTAACTTCATGGTTTTGCATTTTTATAAATATTTGGAAAACAAAAGTTGAGAACTTTACCAAGTGGGTGATTAGAAATTTAGATTATAGGTTTAAATCTATTTTAATTCTAAGCTTTTAGAATCTCTAAACTTTTTAAATATCTATTTCCATAACTTTTTCGTTAAAAAATTATTTTAATAAGGATCGTCTTGGTCTCTTCAATATTATTCTACCACTCAATATCTACTTTAGTCAATCTACATGGCTCCTATATATATTTATGTCAACGTGTTAGTTTACACATTTTGAATCACATATACTTTTCGAGATTGGAGACTTAAATCCAAACAAATAATAATAAATAAATAATGACATTACTATATTTTTTAAGTTTAGAAACTAAAGTAGACATTTTGAGAATTTAAACGCTAAAAATAAAATCGCGTAGAACAAATTTTAAAAATCAATGAAAAAAAAGTTTGAACTTCTAGTCAAAATCATATTTTGCAAATTAACAAAAAAAAATTGTATAAAAAATACAGCAAAGCCATGGAAGAAGAGTACATACCTCAAGTGATAAAGATAGCAGAGAAGCTTGCAGAGCTAATGTGCGAGAATTTGGGCCTAGAAAAAGCCCACATAAAGCAGCTTTTCAACGGAACCAAAGGCCCATCCGTTGGAACAAAAGTGGCGAAATACCCAAAATGCCCTCGGCCGGATCTGGTGAGAGGGCTCCGGGAGCACACCGACGCTGGCGGAATAATCCTCCTGCTGCAGGACGACCAAGTCCCAGGCCTGGAGTTCCTAAACGACGGCGTATGGTTCAAAATCCCGCCGTCGAAGAACAACGCCATATTCGTCAACATCGGCGATCAGATCGAGGTTCTGAGCAACGGCAAATACAAGAGCATTTTGCACCGCGTTCTGGCCGAGAAGGACGGCAGCAGGCTCTCCATTGCCACGTTTTATAATCCGGCCGCCGACGCCGTCATTTCGCCGGCGGCGGAGCTTCTGTATCCCGGCGGGTACGGGTTTGAGGACTATTTGAAGTTTTATAACACTACAAAATTTGGGGATAAAGAGAAGAGGTTTCAATCGATGATGAGAATGTGA

mRNA sequence

ATGAAGATTCCTACCATTGATTTCAATCAGCTCACTGGAGACAACAGGACCAAAACCCTGTCTGTCCTTCATGATGCTTGCAAAGATTGGGGCTTCTTTTTGGTAGAGAACCATGGGATTGAAGAGGAAGTATTGGAGAAAGTGAAGCTGTTGGTAAACAGACATTATGATGAGAATATGAAAGAAAAATTTTATAATTCAGATGTGGCCAAAAACTTGGGCAAAATGGCAGTATCTGATGTGGATTGGGAAAGCACTTTCTTCATTTGGCACAAACCAAACTCTAACATCTCTCATTTTACCAATGTCTCACAAGATCTTTGCAAAGCCATGGAAGAAGAGTACATACCTCAAGTGATAAAGATAGCAGAGAAGCTTGCAGAGCTAATGTGCGAGAATTTGGGCCTAGAAAAAGCCCACATAAAGCAGCTTTTCAACGGAACCAAAGGCCCATCCGTTGGAACAAAAGTGGCGAAATACCCAAAATGCCCTCGGCCGGATCTGGTGAGAGGGCTCCGGGAGCACACCGACGCTGGCGGAATAATCCTCCTGCTGCAGGACGACCAAGTCCCAGGCCTGGAGTTCCTAAACGACGGCGTATGGTTCAAAATCCCGCCGTCGAAGAACAACGCCATATTCGTCAACATCGGCGATCAGATCGAGGTTCTGAGCAACGGCAAATACAAGAGCATTTTGCACCGCGTTCTGGCCGAGAAGGACGGCAGCAGGCTCTCCATTGCCACGTTTTATAATCCGGCCGCCGACGCCGTCATTTCGCCGGCGGCGGAGCTTCTGTATCCCGGCGGGTACGGGTTTGAGGACTATTTGAAGTTTTATAACACTACAAAATTTGGGGATAAAGAGAAGAGGTTTCAATCGATGATGAGAATGTGA

Coding sequence (CDS)

ATGAAGATTCCTACCATTGATTTCAATCAGCTCACTGGAGACAACAGGACCAAAACCCTGTCTGTCCTTCATGATGCTTGCAAAGATTGGGGCTTCTTTTTGGTAGAGAACCATGGGATTGAAGAGGAAGTATTGGAGAAAGTGAAGCTGTTGGTAAACAGACATTATGATGAGAATATGAAAGAAAAATTTTATAATTCAGATGTGGCCAAAAACTTGGGCAAAATGGCAGTATCTGATGTGGATTGGGAAAGCACTTTCTTCATTTGGCACAAACCAAACTCTAACATCTCTCATTTTACCAATGTCTCACAAGATCTTTGCAAAGCCATGGAAGAAGAGTACATACCTCAAGTGATAAAGATAGCAGAGAAGCTTGCAGAGCTAATGTGCGAGAATTTGGGCCTAGAAAAAGCCCACATAAAGCAGCTTTTCAACGGAACCAAAGGCCCATCCGTTGGAACAAAAGTGGCGAAATACCCAAAATGCCCTCGGCCGGATCTGGTGAGAGGGCTCCGGGAGCACACCGACGCTGGCGGAATAATCCTCCTGCTGCAGGACGACCAAGTCCCAGGCCTGGAGTTCCTAAACGACGGCGTATGGTTCAAAATCCCGCCGTCGAAGAACAACGCCATATTCGTCAACATCGGCGATCAGATCGAGGTTCTGAGCAACGGCAAATACAAGAGCATTTTGCACCGCGTTCTGGCCGAGAAGGACGGCAGCAGGCTCTCCATTGCCACGTTTTATAATCCGGCCGCCGACGCCGTCATTTCGCCGGCGGCGGAGCTTCTGTATCCCGGCGGGTACGGGTTTGAGGACTATTTGAAGTTTTATAACACTACAAAATTTGGGGATAAAGAGAAGAGGTTTCAATCGATGATGAGAATGTGA

Protein sequence

MKIPTIDFNQLTGDNRTKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHYDENMKEKFYNSDVAKNLGKMAVSDVDWESTFFIWHKPNSNISHFTNVSQDLCKAMEEEYIPQVIKIAEKLAELMCENLGLEKAHIKQLFNGTKGPSVGTKVAKYPKCPRPDLVRGLREHTDAGGIILLLQDDQVPGLEFLNDGVWFKIPPSKNNAIFVNIGDQIEVLSNGKYKSILHRVLAEKDGSRLSIATFYNPAADAVISPAAELLYPGGYGFEDYLKFYNTTKFGDKEKRFQSMMRM
Homology
BLAST of Lag0007032 vs. NCBI nr
Match: XP_038890644.1 (1-aminocyclopropane-1-carboxylate oxidase 1 [Benincasa hispida])

HSP 1 Score: 540.4 bits (1391), Expect = 9.6e-150
Identity = 257/296 (86.82%), Postives = 276/296 (93.24%), Query Frame = 0

Query: 1   MKIPTIDFNQLTGDNRTKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHYDENM 60
           MKIPTIDFNQLTGDN+   LSVLH+AC++WGFFLVENHGIEE VLEKVK LVN+HYD+NM
Sbjct: 3   MKIPTIDFNQLTGDNKANALSVLHEACQNWGFFLVENHGIEERVLEKVKFLVNKHYDDNM 62

Query: 61  KEKFYNSDVAKNLGKMAVSDVDWESTFFIWHKPNSNISHFTNVSQDLCKAMEEEYIPQVI 120
           KE+FYNSDVAKNLGKMA+++VDWESTFFI HKP SN+SHFTN+SQ LCKAMEEEYIP+VI
Sbjct: 63  KEEFYNSDVAKNLGKMALTNVDWESTFFICHKPTSNLSHFTNISQHLCKAMEEEYIPEVI 122

Query: 121 KIAEKLAELMCENLGLEKAHIKQLFNGTKGPSVGTKVAKYPKCPRPDLVRGLREHTDAGG 180
           KIAEKLAELM ENLGL+KAHIKQLFNGT GPSVGTKVAKYP+CPRP+LVRGLREHTDAGG
Sbjct: 123 KIAEKLAELMSENLGLQKAHIKQLFNGTNGPSVGTKVAKYPECPRPELVRGLREHTDAGG 182

Query: 181 IILLLQDDQVPGLEFLNDGVWFKIPPSKNNAIFVNIGDQIEVLSNGKYKSILHRVLAEKD 240
           IILLLQDDQVPGLEF  DG WFKIPPSKNNAIFVNIGDQIEVLSNGKYKSILHRVLAEKD
Sbjct: 183 IILLLQDDQVPGLEFQIDGGWFKIPPSKNNAIFVNIGDQIEVLSNGKYKSILHRVLAEKD 242

Query: 241 GSRLSIATFYNPAADAVISPAAELLYPGGYGFEDYLKFYNTTKFGDKEKRFQSMMR 297
           GSRLSIATFYNPA DA+ISPA ELLYPGGY F DYLKFYNTTKFGDKE+RF   M+
Sbjct: 243 GSRLSIATFYNPATDALISPAPELLYPGGYRFGDYLKFYNTTKFGDKERRFSDFMK 298

BLAST of Lag0007032 vs. NCBI nr
Match: XP_022138962.1 (1-aminocyclopropane-1-carboxylate oxidase 1 [Momordica charantia])

HSP 1 Score: 532.7 bits (1371), Expect = 2.0e-147
Identity = 253/297 (85.19%), Postives = 276/297 (92.93%), Query Frame = 0

Query: 1   MKIPTIDFNQLTGDNRTKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHYDENM 60
           M+IPTIDFNQL GDNR KTLS+LH+AC++WGFF+VENHGIEEEVLEKVK LVNRHYDENM
Sbjct: 1   MEIPTIDFNQLNGDNRAKTLSLLHEACQNWGFFMVENHGIEEEVLEKVKELVNRHYDENM 60

Query: 61  KEKFYNSDVAKNLGKMAVSDVDWESTFFIWHKPNSNISHFTNVSQDLCKAMEEEYIPQVI 120
           KE FY+S+VAKNLGKM +SDVDWESTFFI H+P SNIS  TN+SQ LC+AMEE+YIPQ+I
Sbjct: 61  KESFYSSEVAKNLGKMPLSDVDWESTFFICHRPKSNISQITNISQHLCEAMEEDYIPQLI 120

Query: 121 KIAEKLAELMCENLGLEKAHIKQLFNGTKGPSVGTKVAKYPKCPRPDLVRGLREHTDAGG 180
           KIAE LAELMCENLGLEKAHIKQLF+GT GPSVGTKVAKYP+CPRP+LVRGLREHTDAGG
Sbjct: 121 KIAETLAELMCENLGLEKAHIKQLFSGTNGPSVGTKVAKYPECPRPELVRGLREHTDAGG 180

Query: 181 IILLLQDDQVPGLEFLNDGVWFKIPPSKNNAIFVNIGDQIEVLSNGKYKSILHRVLAEKD 240
           IILLLQDDQVPGLEF NDGVW KIPPSK NAIFVNIGDQIEV+SNG+YKSILHRVLAEK+
Sbjct: 181 IILLLQDDQVPGLEFQNDGVWLKIPPSKKNAIFVNIGDQIEVVSNGRYKSILHRVLAEKE 240

Query: 241 GSRLSIATFYNPAADAVISPAAELLYPGGYGFEDYLKFYNTTKFGDKEKRFQSMMRM 298
           GSR+SIATFYNPA DAVISPA ELLYPG Y FEDYLKFYNTTKFGDK +RFQSM +M
Sbjct: 241 GSRISIATFYNPATDAVISPAPELLYPGEYRFEDYLKFYNTTKFGDKGRRFQSMKKM 297

BLAST of Lag0007032 vs. NCBI nr
Match: XP_022965527.1 (1-aminocyclopropane-1-carboxylate oxidase 1 [Cucurbita maxima])

HSP 1 Score: 524.6 bits (1350), Expect = 5.4e-145
Identity = 249/297 (83.84%), Postives = 271/297 (91.25%), Query Frame = 0

Query: 1   MKIPTIDFNQLTGDNRTKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHYDENM 60
           MKIPTIDF+++ GDNR + LSVLHDAC +WGFF++ENHGIEE VLEKVKLLVN HYDE M
Sbjct: 1   MKIPTIDFSEVNGDNRARALSVLHDACLNWGFFMIENHGIEEAVLEKVKLLVNTHYDETM 60

Query: 61  KEKFYNSDVAKNLGKMAVSDVDWESTFFIWHKPNSNISHFTNVSQDLCKAMEEEYIPQVI 120
           K+KFY+S+VAKNLGK+ + DVDWESTFFI HKP SNIS F N+S  LCKAMEEEYIPQ++
Sbjct: 61  KDKFYDSEVAKNLGKIQLFDVDWESTFFISHKPTSNISRFPNISDHLCKAMEEEYIPQLV 120

Query: 121 KIAEKLAELMCENLGLEKAHIKQLFNGTKGPSVGTKVAKYPKCPRPDLVRGLREHTDAGG 180
            IAEKLAELMCENLGL+K +IKQLFNGTKGPSVGTKVAKYP+CPRP+LVRGLREHTDAGG
Sbjct: 121 MIAEKLAELMCENLGLQKTYIKQLFNGTKGPSVGTKVAKYPECPRPELVRGLREHTDAGG 180

Query: 181 IILLLQDDQVPGLEFLNDGVWFKIPPSKNNAIFVNIGDQIEVLSNGKYKSILHRVLAEKD 240
           IILLLQDD+VPGLEF  DG WFKIPPSKNNAIFVNIGDQIEVL+NGKYKSILHRVLAEK+
Sbjct: 181 IILLLQDDKVPGLEFQKDGEWFKIPPSKNNAIFVNIGDQIEVLTNGKYKSILHRVLAEKE 240

Query: 241 GSRLSIATFYNPAADAVISPAAELLYPGGYGFEDYLKFYNTTKFGDKEKRFQSMMRM 298
           GSRLSIATFYNPA DAVISPAAE LYPGGY FEDYLKFYNTTKFGDKEKRF SM  M
Sbjct: 241 GSRLSIATFYNPATDAVISPAAEALYPGGYRFEDYLKFYNTTKFGDKEKRFGSMKNM 297

BLAST of Lag0007032 vs. NCBI nr
Match: TYK10774.1 (1-aminocyclopropane-1-carboxylate oxidase 1 [Cucumis melo var. makuwa])

HSP 1 Score: 523.1 bits (1346), Expect = 1.6e-144
Identity = 251/300 (83.67%), Postives = 274/300 (91.33%), Query Frame = 0

Query: 1   MKIPTIDFNQLTGDNRTKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHYDENM 60
           MKIPTIDFNQL  DN+  TLS+LH+AC++WGFFLVENHGIEE VLEKVKLLVN+HYDENM
Sbjct: 1   MKIPTIDFNQLNEDNKGNTLSILHEACQNWGFFLVENHGIEERVLEKVKLLVNKHYDENM 60

Query: 61  KEKFYNSDVAKNLGKMAVSDVDWESTFFIWHKPNSNISHFTNVSQDLCKAMEEEYIPQVI 120
           KE+FYNSDVAKNLGK+ +S+VDWESTFFI HKP SN+S F N+SQ LCKAMEEEYIP++I
Sbjct: 61  KEEFYNSDVAKNLGKITLSNVDWESTFFISHKPTSNVSQFCNISQPLCKAMEEEYIPEII 120

Query: 121 KIAEKLAELMCENLGLEKAHIKQLFNGTKGPSVGTKVAKYPKCPRPDLVRGLREHTDAGG 180
           KIAEKL+ELMCENLGL+K+HIKQLFNGT GPSVGTKVAKYP+CP+PDLVRGLREHTDAGG
Sbjct: 121 KIAEKLSELMCENLGLQKSHIKQLFNGTNGPSVGTKVAKYPECPQPDLVRGLREHTDAGG 180

Query: 181 IILLLQDDQVPGLEFLNDGVWFKIPPSKNNAIFVNIGDQIEVLSNGKYKSILHRVLAEKD 240
           IILLLQDDQVPGLEF  DG WFKIPPSKNNAIFVNIGDQIEVL+NGKYKSILHRVLAEK 
Sbjct: 181 IILLLQDDQVPGLEFQIDGRWFKIPPSKNNAIFVNIGDQIEVLTNGKYKSILHRVLAEKH 240

Query: 241 GSRLSIATFYNPAADAVISPAAELLYPGGYGFEDYLKFYNTTKFGDKEKR---FQSMMRM 298
           GSRLSIATFYNPA DA+I PA EL YPGGY F DYLKFYNTTKFGDKEKR   FQS+ +M
Sbjct: 241 GSRLSIATFYNPATDALIFPAPELSYPGGYRFGDYLKFYNTTKFGDKEKRFSDFQSINKM 300

BLAST of Lag0007032 vs. NCBI nr
Match: KAG7021341.1 (1-aminocyclopropane-1-carboxylate oxidase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 522.3 bits (1344), Expect = 2.7e-144
Identity = 246/294 (83.67%), Postives = 270/294 (91.84%), Query Frame = 0

Query: 1   MKIPTIDFNQLTGDNRTKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHYDENM 60
           MKIPTIDF+++ GD R + LSVLHDAC +WGFF++ENHGIEE VLEKVKLLVN HYDE M
Sbjct: 3   MKIPTIDFSEVNGDKRARALSVLHDACLNWGFFMIENHGIEEAVLEKVKLLVNTHYDETM 62

Query: 61  KEKFYNSDVAKNLGKMAVSDVDWESTFFIWHKPNSNISHFTNVSQDLCKAMEEEYIPQVI 120
           K+KFY+S++AKNLGK+ +SDVDWES FFI HKP SNIS F N+S  LCKAMEEEYIPQ++
Sbjct: 63  KDKFYDSEMAKNLGKIPLSDVDWESMFFISHKPTSNISRFPNISDHLCKAMEEEYIPQLV 122

Query: 121 KIAEKLAELMCENLGLEKAHIKQLFNGTKGPSVGTKVAKYPKCPRPDLVRGLREHTDAGG 180
            IAEKLAELMCENLGL+K HIKQLFNGTKGPSVGTKVAKYP+CPRP+LVRGLREHTDAGG
Sbjct: 123 MIAEKLAELMCENLGLQKTHIKQLFNGTKGPSVGTKVAKYPECPRPELVRGLREHTDAGG 182

Query: 181 IILLLQDDQVPGLEFLNDGVWFKIPPSKNNAIFVNIGDQIEVLSNGKYKSILHRVLAEKD 240
           IILLLQDD+VPGLEF  DG WFKIPPSKNNAIFVNIGDQIEVL+NGKYKS+LHRVLAEK+
Sbjct: 183 IILLLQDDKVPGLEFQKDGEWFKIPPSKNNAIFVNIGDQIEVLTNGKYKSVLHRVLAEKE 242

Query: 241 GSRLSIATFYNPAADAVISPAAELLYPGGYGFEDYLKFYNTTKFGDKEKRFQSM 295
           GSRLSIATFYNPA+DAVISPAAE LYPGGY FEDYLKFYNTTKFGDKEKRF SM
Sbjct: 243 GSRLSIATFYNPASDAVISPAAEALYPGGYTFEDYLKFYNTTKFGDKEKRFGSM 296

BLAST of Lag0007032 vs. ExPASy Swiss-Prot
Match: Q9ZUN4 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=ACO1 PE=2 SV=1)

HSP 1 Score: 417.9 bits (1073), Expect = 9.4e-116
Identity = 191/297 (64.31%), Postives = 241/297 (81.14%), Query Frame = 0

Query: 1   MKIPTIDFNQLTGDNRTKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHYDENM 60
           M+IP IDF +L G+ R+KT+S+L  AC  WGFF+V+NHGI++E++EKVK ++N HY+E++
Sbjct: 9   MEIPVIDFAELDGEKRSKTMSLLDHACDKWGFFMVDNHGIDKELMEKVKKMINSHYEEHL 68

Query: 61  KEKFYNSDVAKNLGKMAVSDVDWESTFFIWHKPNSNISHFTNVSQDLCKAMEEEYIPQVI 120
           KEKFY S++ K L +   SD DWES+FFI HKP SNI    N+S++L K M +EY+ Q+ 
Sbjct: 69  KEKFYQSEMVKALSEGKTSDADWESSFFISHKPTSNICQIPNISEELSKTM-DEYVCQLH 128

Query: 121 KIAEKLAELMCENLGLEKAHIKQLFNGTKGPSVGTKVAKYPKCPRPDLVRGLREHTDAGG 180
           K AE+L++LMCENLGL++  I   F+G KGP+ GTKVAKYP+CPRP+L+RGLREHTDAGG
Sbjct: 129 KFAERLSKLMCENLGLDQEDIMNAFSGPKGPAFGTKVAKYPECPRPELMRGLREHTDAGG 188

Query: 181 IILLLQDDQVPGLEFLNDGVWFKIPPSKNNAIFVNIGDQIEVLSNGKYKSILHRVLAEKD 240
           IILLLQDDQVPGLEF  DG W  IPPSKNN IFVN GDQ+E+LSNG+YKS++HRV+  K 
Sbjct: 189 IILLLQDDQVPGLEFFKDGKWVPIPPSKNNTIFVNTGDQLEILSNGRYKSVVHRVMTVKH 248

Query: 241 GSRLSIATFYNPAADAVISPAAELLYPGGYGFEDYLKFYNTTKFGDKEKRFQSMMRM 298
           GSRLSIATFYNPA DA+ISPA +LLYP GY F+DYLK Y+TTKFGDK  R ++M +M
Sbjct: 249 GSRLSIATFYNPAGDAIISPAPKLLYPSGYRFQDYLKLYSTTKFGDKGPRLETMKKM 304

BLAST of Lag0007032 vs. ExPASy Swiss-Prot
Match: P31237 (1-aminocyclopropane-1-carboxylate oxidase OS=Actinidia deliciosa OX=3627 GN=ACO PE=2 SV=1)

HSP 1 Score: 314.3 bits (804), Expect = 1.5e-84
Identity = 151/306 (49.35%), Postives = 216/306 (70.59%), Query Frame = 0

Query: 4   PTIDFNQLTGDNRTKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHYDENMKEK 63
           P ID  +L G+ R  T+  + DAC++WGFF + NHGI  E+++ V+ L   HY++ M+++
Sbjct: 5   PVIDMEKLNGEERAPTMEKIKDACENWGFFELVNHGISHELMDTVERLTKEHYNKCMEQR 64

Query: 64  FYNSDVAKNLG--KMAVSDVDWESTFFIWHKPNSNISHFTNVSQDLCKAMEEEYIPQVIK 123
           F      K L   +  ++D+DWESTFF+ H P SNIS   ++ QD  KAM +E+  ++ K
Sbjct: 65  FKEMVATKGLEAVQSEINDLDWESTFFLRHLPVSNISEIPDLEQDHRKAM-KEFAEKLEK 124

Query: 124 IAEKLAELMCENLGLEKAHIKQLFNGTKGPSVGTKVAKYPKCPRPDLVRGLREHTDAGGI 183
           +AE+L +L+CEN+GLEK ++K+ F G+KGP+ GTKV+ YP CPRP+L++GLR HTDAGGI
Sbjct: 125 LAEQLLDLLCENVGLEKGYLKKAFYGSKGPTFGTKVSNYPPCPRPELIKGLRAHTDAGGI 184

Query: 184 ILLLQDDQVPGLEFLNDGVWFKIPPSKNNAIFVNIGDQIEVLSNGKYKSILHRVLAEKDG 243
           ILL QD++V GL+ L DG W  +PP K ++I +NIGDQ+EV++NGKYKS++HRV+A+ DG
Sbjct: 185 ILLFQDNKVSGLQLLKDGEWIDVPPMK-HSIVINIGDQLEVITNGKYKSVMHRVIAQPDG 244

Query: 244 SRLSIATFYNPAADAVISPAAEL----------LYPGGYGFEDYLKFYNTTKFGDKEKRF 298
           +R+SIA+FYNP +DAV+ PA  L          +YP  + FEDY+K Y   KF  KE RF
Sbjct: 245 NRMSIASFYNPGSDAVMYPAPALVDKEEDQQKQVYP-KFVFEDYMKLYAGLKFQAKEPRF 304

BLAST of Lag0007032 vs. ExPASy Swiss-Prot
Match: O48882 (1-aminocyclopropane-1-carboxylate oxidase 2 OS=Malus domestica OX=3750 GN=ACO2 PE=2 SV=1)

HSP 1 Score: 313.5 bits (802), Expect = 2.5e-84
Identity = 151/302 (50.00%), Postives = 214/302 (70.86%), Query Frame = 0

Query: 4   PTIDFNQLTGDNRTKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHYDENMKEK 63
           P +D + + G+ R  TL  ++DAC++WGFF + NHGI  E+L+ V+ +   HY + M+++
Sbjct: 5   PVVDMDLINGEERAATLEKINDACENWGFFELVNHGISTELLDTVEKMNKDHYKKTMEQR 64

Query: 64  FYNSDVAKNLG--KMAVSDVDWESTFFIWHKPNSNISHFTNVSQDLCKAMEEEYIPQVIK 123
           F     AK L   +  +  +DWESTFF+ H P+SNIS   ++ +D  K M +E+  ++ K
Sbjct: 65  FKEMVAAKGLEAVQSEIHYLDWESTFFLRHLPSSNISEIPDLEEDYRKTM-KEFAVELEK 124

Query: 124 IAEKLAELMCENLGLEKAHIKQLFNGTKGPSVGTKVAKYPKCPRPDLVRGLREHTDAGGI 183
           +AEKL +L+CENLGLEK ++K+ F G+KGP+ GTKV+ YP CP+PDL++GLR HTDAGGI
Sbjct: 125 LAEKLLDLLCENLGLEKGYLKKAFYGSKGPNFGTKVSNYPPCPKPDLIKGLRAHTDAGGI 184

Query: 184 ILLLQDDQVPGLEFLNDGVWFKIPPSKNNAIFVNIGDQIEVLSNGKYKSILHRVLAEKDG 243
           ILL QDD+V GL+ L DG W  +PP  +++I +N+GDQIEV++NGKYKSI+HRV+A+ DG
Sbjct: 185 ILLFQDDKVSGLQLLKDGEWMDVPP-VHHSIVINLGDQIEVITNGKYKSIMHRVIAQSDG 244

Query: 244 SRLSIATFYNPAADAVISPAAELL---------YPGGYGFEDYLKFYNTTKFGDKEKRFQ 295
           +R+SIA+FYNP  DA ISPA  LL         YP  + F+DY+K Y+  KF  KE RF+
Sbjct: 245 TRMSIASFYNPGDDAFISPAPALLEEKSEVSPTYP-KFLFDDYMKLYSGLKFQAKEPRFE 303

BLAST of Lag0007032 vs. ExPASy Swiss-Prot
Match: Q41931 (1-aminocyclopropane-1-carboxylate oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=ACO2 PE=1 SV=2)

HSP 1 Score: 312.8 bits (800), Expect = 4.3e-84
Identity = 141/301 (46.84%), Postives = 220/301 (73.09%), Query Frame = 0

Query: 1   MKIPTIDFNQLTGDNRTKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHYDENM 60
           MK P +D ++L G+ R +T++++++AC++WGFF + NHG+  ++++K++ +   HY    
Sbjct: 5   MKFPVVDLSKLNGEERDQTMALINEACENWGFFEIVNHGLPHDLMDKIEKMTKDHYKTCQ 64

Query: 61  KEKFYNSDVAKNLGKM--AVSDVDWESTFFIWHKPNSNISHFTNVSQDLCKAMEEEYIPQ 120
           ++KF +   +K L  +   V DVDWESTF++ H P SN++  ++VS +   AM +++  +
Sbjct: 65  EQKFNDMLKSKGLDNLETEVEDVDWESTFYVRHLPQSNLNDISDVSDEYRTAM-KDFGKR 124

Query: 121 VIKIAEKLAELMCENLGLEKAHIKQLFNGTKGPSVGTKVAKYPKCPRPDLVRGLREHTDA 180
           +  +AE L +L+CENLGLEK ++K++F+GTKGP+ GTKV+ YP CP+P++++GLR HTDA
Sbjct: 125 LENLAEDLLDLLCENLGLEKGYLKKVFHGTKGPTFGTKVSNYPPCPKPEMIKGLRAHTDA 184

Query: 181 GGIILLLQDDQVPGLEFLNDGVWFKIPPSKNNAIFVNIGDQIEVLSNGKYKSILHRVLAE 240
           GGIILL QDD+V GL+ L DG W  +PP  N++I +N+GDQ+EV++NGKYKS+LHRV+ +
Sbjct: 185 GGIILLFQDDKVSGLQLLKDGDWIDVPP-LNHSIVINLGDQLEVITNGKYKSVLHRVVTQ 244

Query: 241 KDGSRLSIATFYNPAADAVISPAAELL-----YPGGYGFEDYLKFYNTTKFGDKEKRFQS 295
           ++G+R+S+A+FYNP +DA ISPA  L+     YP  + F+DY+K Y   KF  KE RF +
Sbjct: 245 QEGNRMSVASFYNPGSDAEISPATSLVEKDSEYP-SFVFDDYMKLYAGVKFQPKEPRFAA 302

BLAST of Lag0007032 vs. ExPASy Swiss-Prot
Match: Q00985 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Malus domestica OX=3750 PE=1 SV=1)

HSP 1 Score: 312.4 bits (799), Expect = 5.6e-84
Identity = 146/302 (48.34%), Postives = 216/302 (71.52%), Query Frame = 0

Query: 4   PTIDFNQLTGDNRTKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHYDENMKEK 63
           P +D + + G+ R  TL  ++DAC++WGFF + NHG+  E+L+ V+ +   HY + M+++
Sbjct: 5   PVVDLSLVNGEERAATLEKINDACENWGFFELVNHGMSTELLDTVEKMTKDHYKKTMEQR 64

Query: 64  FYNSDVAKNLG--KMAVSDVDWESTFFIWHKPNSNISHFTNVSQDLCKAMEEEYIPQVIK 123
           F     AK L   +  + D+DWESTFF+ H P+SNIS   ++ ++  K M +E+  ++ K
Sbjct: 65  FKEMVAAKGLDDVQSEIHDLDWESTFFLRHLPSSNISEIPDLEEEYRKTM-KEFAVELEK 124

Query: 124 IAEKLAELMCENLGLEKAHIKQLFNGTKGPSVGTKVAKYPKCPRPDLVRGLREHTDAGGI 183
           +AEKL +L+CENLGLEK ++K++F G+KGP+ GTKV+ YP CP+PDL++GLR H+DAGGI
Sbjct: 125 LAEKLLDLLCENLGLEKGYLKKVFYGSKGPNFGTKVSNYPPCPKPDLIKGLRAHSDAGGI 184

Query: 184 ILLLQDDQVPGLEFLNDGVWFKIPPSKNNAIFVNIGDQIEVLSNGKYKSILHRVLAEKDG 243
           ILL QDD+V GL+ L DG W  +PP  +++I +N+GDQIEV++NGKYKS++HRV+A+ DG
Sbjct: 185 ILLFQDDKVSGLQLLKDGEWVDVPP-MHHSIVINLGDQIEVITNGKYKSVMHRVIAQSDG 244

Query: 244 SRLSIATFYNPAADAVISPAAELL---------YPGGYGFEDYLKFYNTTKFGDKEKRFQ 295
           +R+SIA+FYNP  D+ ISPA  +L         YP  + F+DY+K Y+  KF  KE RF+
Sbjct: 245 TRMSIASFYNPGNDSFISPAPAVLEKKTEDAPTYP-KFVFDDYMKLYSGLKFQAKEPRFE 303

BLAST of Lag0007032 vs. ExPASy TrEMBL
Match: A0A6J1CCP2 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Momordica charantia OX=3673 GN=LOC111010009 PE=3 SV=1)

HSP 1 Score: 532.7 bits (1371), Expect = 9.7e-148
Identity = 253/297 (85.19%), Postives = 276/297 (92.93%), Query Frame = 0

Query: 1   MKIPTIDFNQLTGDNRTKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHYDENM 60
           M+IPTIDFNQL GDNR KTLS+LH+AC++WGFF+VENHGIEEEVLEKVK LVNRHYDENM
Sbjct: 1   MEIPTIDFNQLNGDNRAKTLSLLHEACQNWGFFMVENHGIEEEVLEKVKELVNRHYDENM 60

Query: 61  KEKFYNSDVAKNLGKMAVSDVDWESTFFIWHKPNSNISHFTNVSQDLCKAMEEEYIPQVI 120
           KE FY+S+VAKNLGKM +SDVDWESTFFI H+P SNIS  TN+SQ LC+AMEE+YIPQ+I
Sbjct: 61  KESFYSSEVAKNLGKMPLSDVDWESTFFICHRPKSNISQITNISQHLCEAMEEDYIPQLI 120

Query: 121 KIAEKLAELMCENLGLEKAHIKQLFNGTKGPSVGTKVAKYPKCPRPDLVRGLREHTDAGG 180
           KIAE LAELMCENLGLEKAHIKQLF+GT GPSVGTKVAKYP+CPRP+LVRGLREHTDAGG
Sbjct: 121 KIAETLAELMCENLGLEKAHIKQLFSGTNGPSVGTKVAKYPECPRPELVRGLREHTDAGG 180

Query: 181 IILLLQDDQVPGLEFLNDGVWFKIPPSKNNAIFVNIGDQIEVLSNGKYKSILHRVLAEKD 240
           IILLLQDDQVPGLEF NDGVW KIPPSK NAIFVNIGDQIEV+SNG+YKSILHRVLAEK+
Sbjct: 181 IILLLQDDQVPGLEFQNDGVWLKIPPSKKNAIFVNIGDQIEVVSNGRYKSILHRVLAEKE 240

Query: 241 GSRLSIATFYNPAADAVISPAAELLYPGGYGFEDYLKFYNTTKFGDKEKRFQSMMRM 298
           GSR+SIATFYNPA DAVISPA ELLYPG Y FEDYLKFYNTTKFGDK +RFQSM +M
Sbjct: 241 GSRISIATFYNPATDAVISPAPELLYPGEYRFEDYLKFYNTTKFGDKGRRFQSMKKM 297

BLAST of Lag0007032 vs. ExPASy TrEMBL
Match: A0A6J1HKJ9 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Cucurbita maxima OX=3661 GN=LOC111465402 PE=3 SV=1)

HSP 1 Score: 524.6 bits (1350), Expect = 2.6e-145
Identity = 249/297 (83.84%), Postives = 271/297 (91.25%), Query Frame = 0

Query: 1   MKIPTIDFNQLTGDNRTKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHYDENM 60
           MKIPTIDF+++ GDNR + LSVLHDAC +WGFF++ENHGIEE VLEKVKLLVN HYDE M
Sbjct: 1   MKIPTIDFSEVNGDNRARALSVLHDACLNWGFFMIENHGIEEAVLEKVKLLVNTHYDETM 60

Query: 61  KEKFYNSDVAKNLGKMAVSDVDWESTFFIWHKPNSNISHFTNVSQDLCKAMEEEYIPQVI 120
           K+KFY+S+VAKNLGK+ + DVDWESTFFI HKP SNIS F N+S  LCKAMEEEYIPQ++
Sbjct: 61  KDKFYDSEVAKNLGKIQLFDVDWESTFFISHKPTSNISRFPNISDHLCKAMEEEYIPQLV 120

Query: 121 KIAEKLAELMCENLGLEKAHIKQLFNGTKGPSVGTKVAKYPKCPRPDLVRGLREHTDAGG 180
            IAEKLAELMCENLGL+K +IKQLFNGTKGPSVGTKVAKYP+CPRP+LVRGLREHTDAGG
Sbjct: 121 MIAEKLAELMCENLGLQKTYIKQLFNGTKGPSVGTKVAKYPECPRPELVRGLREHTDAGG 180

Query: 181 IILLLQDDQVPGLEFLNDGVWFKIPPSKNNAIFVNIGDQIEVLSNGKYKSILHRVLAEKD 240
           IILLLQDD+VPGLEF  DG WFKIPPSKNNAIFVNIGDQIEVL+NGKYKSILHRVLAEK+
Sbjct: 181 IILLLQDDKVPGLEFQKDGEWFKIPPSKNNAIFVNIGDQIEVLTNGKYKSILHRVLAEKE 240

Query: 241 GSRLSIATFYNPAADAVISPAAELLYPGGYGFEDYLKFYNTTKFGDKEKRFQSMMRM 298
           GSRLSIATFYNPA DAVISPAAE LYPGGY FEDYLKFYNTTKFGDKEKRF SM  M
Sbjct: 241 GSRLSIATFYNPATDAVISPAAEALYPGGYRFEDYLKFYNTTKFGDKEKRFGSMKNM 297

BLAST of Lag0007032 vs. ExPASy TrEMBL
Match: A0A5D3CHV3 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G00870 PE=3 SV=1)

HSP 1 Score: 523.1 bits (1346), Expect = 7.7e-145
Identity = 251/300 (83.67%), Postives = 274/300 (91.33%), Query Frame = 0

Query: 1   MKIPTIDFNQLTGDNRTKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHYDENM 60
           MKIPTIDFNQL  DN+  TLS+LH+AC++WGFFLVENHGIEE VLEKVKLLVN+HYDENM
Sbjct: 1   MKIPTIDFNQLNEDNKGNTLSILHEACQNWGFFLVENHGIEERVLEKVKLLVNKHYDENM 60

Query: 61  KEKFYNSDVAKNLGKMAVSDVDWESTFFIWHKPNSNISHFTNVSQDLCKAMEEEYIPQVI 120
           KE+FYNSDVAKNLGK+ +S+VDWESTFFI HKP SN+S F N+SQ LCKAMEEEYIP++I
Sbjct: 61  KEEFYNSDVAKNLGKITLSNVDWESTFFISHKPTSNVSQFCNISQPLCKAMEEEYIPEII 120

Query: 121 KIAEKLAELMCENLGLEKAHIKQLFNGTKGPSVGTKVAKYPKCPRPDLVRGLREHTDAGG 180
           KIAEKL+ELMCENLGL+K+HIKQLFNGT GPSVGTKVAKYP+CP+PDLVRGLREHTDAGG
Sbjct: 121 KIAEKLSELMCENLGLQKSHIKQLFNGTNGPSVGTKVAKYPECPQPDLVRGLREHTDAGG 180

Query: 181 IILLLQDDQVPGLEFLNDGVWFKIPPSKNNAIFVNIGDQIEVLSNGKYKSILHRVLAEKD 240
           IILLLQDDQVPGLEF  DG WFKIPPSKNNAIFVNIGDQIEVL+NGKYKSILHRVLAEK 
Sbjct: 181 IILLLQDDQVPGLEFQIDGRWFKIPPSKNNAIFVNIGDQIEVLTNGKYKSILHRVLAEKH 240

Query: 241 GSRLSIATFYNPAADAVISPAAELLYPGGYGFEDYLKFYNTTKFGDKEKR---FQSMMRM 298
           GSRLSIATFYNPA DA+I PA EL YPGGY F DYLKFYNTTKFGDKEKR   FQS+ +M
Sbjct: 241 GSRLSIATFYNPATDALIFPAPELSYPGGYRFGDYLKFYNTTKFGDKEKRFSDFQSINKM 300

BLAST of Lag0007032 vs. ExPASy TrEMBL
Match: A0A1S3CB09 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Cucumis melo OX=3656 GN=LOC103498449 PE=3 SV=1)

HSP 1 Score: 521.9 bits (1343), Expect = 1.7e-144
Identity = 250/300 (83.33%), Postives = 274/300 (91.33%), Query Frame = 0

Query: 1   MKIPTIDFNQLTGDNRTKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHYDENM 60
           MKIPTIDFNQL  DN+  TLS+LH+AC++WGFFLVENHGIEE VLEKVKLLVN+HYDENM
Sbjct: 1   MKIPTIDFNQLNEDNKGNTLSILHEACQNWGFFLVENHGIEERVLEKVKLLVNKHYDENM 60

Query: 61  KEKFYNSDVAKNLGKMAVSDVDWESTFFIWHKPNSNISHFTNVSQDLCKAMEEEYIPQVI 120
           KE+FYNSDVAKNLGK+ +S++DWESTFFI HKP SN+S F N+SQ LCKAMEEEYIP++I
Sbjct: 61  KEEFYNSDVAKNLGKITLSNMDWESTFFISHKPTSNVSQFCNISQPLCKAMEEEYIPEII 120

Query: 121 KIAEKLAELMCENLGLEKAHIKQLFNGTKGPSVGTKVAKYPKCPRPDLVRGLREHTDAGG 180
           KIAEKL+ELMCENLGL+K+HIKQLFNGT GPSVGTKVAKYP+CP+PDLVRGLREHTDAGG
Sbjct: 121 KIAEKLSELMCENLGLQKSHIKQLFNGTNGPSVGTKVAKYPECPQPDLVRGLREHTDAGG 180

Query: 181 IILLLQDDQVPGLEFLNDGVWFKIPPSKNNAIFVNIGDQIEVLSNGKYKSILHRVLAEKD 240
           IILLLQDDQVPGLEF  DG WFKIPPSKNNAIFVNIGDQIEVL+NGKYKSILHRVLAEK 
Sbjct: 181 IILLLQDDQVPGLEFQIDGRWFKIPPSKNNAIFVNIGDQIEVLTNGKYKSILHRVLAEKH 240

Query: 241 GSRLSIATFYNPAADAVISPAAELLYPGGYGFEDYLKFYNTTKFGDKEKR---FQSMMRM 298
           GSRLSIATFYNPA DA+I PA EL YPGGY F DYLKFYNTTKFGDKEKR   FQS+ +M
Sbjct: 241 GSRLSIATFYNPATDALIFPAPELSYPGGYRFGDYLKFYNTTKFGDKEKRFSDFQSINKM 300

BLAST of Lag0007032 vs. ExPASy TrEMBL
Match: A0A6J1FD26 (1-aminocyclopropane-1-carboxylate oxidase 1 OS=Cucurbita moschata OX=3662 GN=LOC111444278 PE=3 SV=1)

HSP 1 Score: 520.8 bits (1340), Expect = 3.8e-144
Identity = 246/297 (82.83%), Postives = 270/297 (90.91%), Query Frame = 0

Query: 1   MKIPTIDFNQLTGDNRTKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHYDENM 60
           MKIPTIDF+++ GDNR + LSVLHDAC +WGFF++ENHGIEE VLEKVKLLVN HYDE M
Sbjct: 3   MKIPTIDFSEVNGDNRARALSVLHDACLNWGFFMIENHGIEEAVLEKVKLLVNAHYDETM 62

Query: 61  KEKFYNSDVAKNLGKMAVSDVDWESTFFIWHKPNSNISHFTNVSQDLCKAMEEEYIPQVI 120
           K+KFY+S++AKNLGK+ +SDVDWES FFI HKP SNIS F N+S  LCKAMEEEYIPQ++
Sbjct: 63  KDKFYDSEMAKNLGKIPLSDVDWESMFFISHKPTSNISRFPNISDHLCKAMEEEYIPQLV 122

Query: 121 KIAEKLAELMCENLGLEKAHIKQLFNGTKGPSVGTKVAKYPKCPRPDLVRGLREHTDAGG 180
            IAEKLAELMCENLGL+K HIKQLFNGTKGPSVGTKVAKYP+CP P+LVRGLREHTDAGG
Sbjct: 123 MIAEKLAELMCENLGLQKTHIKQLFNGTKGPSVGTKVAKYPECPLPELVRGLREHTDAGG 182

Query: 181 IILLLQDDQVPGLEFLNDGVWFKIPPSKNNAIFVNIGDQIEVLSNGKYKSILHRVLAEKD 240
           IILLLQDD+VPGLEF  DG WFKIPPSKNNAIFVNIGDQIEVL+NGKYKS+LHRVLAEK+
Sbjct: 183 IILLLQDDKVPGLEFQKDGEWFKIPPSKNNAIFVNIGDQIEVLTNGKYKSVLHRVLAEKE 242

Query: 241 GSRLSIATFYNPAADAVISPAAELLYPGGYGFEDYLKFYNTTKFGDKEKRFQSMMRM 298
           GSRLSIATFYNPA+DAVISPAAE LYPGGY FEDYLKFYNT KFGDKEKRF SM  M
Sbjct: 243 GSRLSIATFYNPASDAVISPAAEALYPGGYTFEDYLKFYNTRKFGDKEKRFGSMKNM 299

BLAST of Lag0007032 vs. TAIR 10
Match: AT2G19590.1 (ACC oxidase 1 )

HSP 1 Score: 417.9 bits (1073), Expect = 6.7e-117
Identity = 191/297 (64.31%), Postives = 241/297 (81.14%), Query Frame = 0

Query: 1   MKIPTIDFNQLTGDNRTKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHYDENM 60
           M+IP IDF +L G+ R+KT+S+L  AC  WGFF+V+NHGI++E++EKVK ++N HY+E++
Sbjct: 9   MEIPVIDFAELDGEKRSKTMSLLDHACDKWGFFMVDNHGIDKELMEKVKKMINSHYEEHL 68

Query: 61  KEKFYNSDVAKNLGKMAVSDVDWESTFFIWHKPNSNISHFTNVSQDLCKAMEEEYIPQVI 120
           KEKFY S++ K L +   SD DWES+FFI HKP SNI    N+S++L K M +EY+ Q+ 
Sbjct: 69  KEKFYQSEMVKALSEGKTSDADWESSFFISHKPTSNICQIPNISEELSKTM-DEYVCQLH 128

Query: 121 KIAEKLAELMCENLGLEKAHIKQLFNGTKGPSVGTKVAKYPKCPRPDLVRGLREHTDAGG 180
           K AE+L++LMCENLGL++  I   F+G KGP+ GTKVAKYP+CPRP+L+RGLREHTDAGG
Sbjct: 129 KFAERLSKLMCENLGLDQEDIMNAFSGPKGPAFGTKVAKYPECPRPELMRGLREHTDAGG 188

Query: 181 IILLLQDDQVPGLEFLNDGVWFKIPPSKNNAIFVNIGDQIEVLSNGKYKSILHRVLAEKD 240
           IILLLQDDQVPGLEF  DG W  IPPSKNN IFVN GDQ+E+LSNG+YKS++HRV+  K 
Sbjct: 189 IILLLQDDQVPGLEFFKDGKWVPIPPSKNNTIFVNTGDQLEILSNGRYKSVVHRVMTVKH 248

Query: 241 GSRLSIATFYNPAADAVISPAAELLYPGGYGFEDYLKFYNTTKFGDKEKRFQSMMRM 298
           GSRLSIATFYNPA DA+ISPA +LLYP GY F+DYLK Y+TTKFGDK  R ++M +M
Sbjct: 249 GSRLSIATFYNPAGDAIISPAPKLLYPSGYRFQDYLKLYSTTKFGDKGPRLETMKKM 304

BLAST of Lag0007032 vs. TAIR 10
Match: AT1G62380.1 (ACC oxidase 2 )

HSP 1 Score: 312.8 bits (800), Expect = 3.0e-85
Identity = 141/301 (46.84%), Postives = 220/301 (73.09%), Query Frame = 0

Query: 1   MKIPTIDFNQLTGDNRTKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHYDENM 60
           MK P +D ++L G+ R +T++++++AC++WGFF + NHG+  ++++K++ +   HY    
Sbjct: 5   MKFPVVDLSKLNGEERDQTMALINEACENWGFFEIVNHGLPHDLMDKIEKMTKDHYKTCQ 64

Query: 61  KEKFYNSDVAKNLGKM--AVSDVDWESTFFIWHKPNSNISHFTNVSQDLCKAMEEEYIPQ 120
           ++KF +   +K L  +   V DVDWESTF++ H P SN++  ++VS +   AM +++  +
Sbjct: 65  EQKFNDMLKSKGLDNLETEVEDVDWESTFYVRHLPQSNLNDISDVSDEYRTAM-KDFGKR 124

Query: 121 VIKIAEKLAELMCENLGLEKAHIKQLFNGTKGPSVGTKVAKYPKCPRPDLVRGLREHTDA 180
           +  +AE L +L+CENLGLEK ++K++F+GTKGP+ GTKV+ YP CP+P++++GLR HTDA
Sbjct: 125 LENLAEDLLDLLCENLGLEKGYLKKVFHGTKGPTFGTKVSNYPPCPKPEMIKGLRAHTDA 184

Query: 181 GGIILLLQDDQVPGLEFLNDGVWFKIPPSKNNAIFVNIGDQIEVLSNGKYKSILHRVLAE 240
           GGIILL QDD+V GL+ L DG W  +PP  N++I +N+GDQ+EV++NGKYKS+LHRV+ +
Sbjct: 185 GGIILLFQDDKVSGLQLLKDGDWIDVPP-LNHSIVINLGDQLEVITNGKYKSVLHRVVTQ 244

Query: 241 KDGSRLSIATFYNPAADAVISPAAELL-----YPGGYGFEDYLKFYNTTKFGDKEKRFQS 295
           ++G+R+S+A+FYNP +DA ISPA  L+     YP  + F+DY+K Y   KF  KE RF +
Sbjct: 245 QEGNRMSVASFYNPGSDAEISPATSLVEKDSEYP-SFVFDDYMKLYAGVKFQPKEPRFAA 302

BLAST of Lag0007032 vs. TAIR 10
Match: AT1G12010.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 303.5 bits (776), Expect = 1.8e-82
Identity = 138/301 (45.85%), Postives = 218/301 (72.43%), Query Frame = 0

Query: 1   MKIPTIDFNQLTGDNRTKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHYDENM 60
           +K P ID ++L G+ R +T++++ DAC++WGFF + NHG+  ++++ ++ +   HY ++M
Sbjct: 5   IKFPVIDLSKLNGEERDQTMALIDDACQNWGFFELVNHGLPYDLMDNIERMTKEHYKKHM 64

Query: 61  KEKFYNSDVAKNLGKM--AVSDVDWESTFFIWHKPNSNISHFTNVSQDLCKAMEEEYIPQ 120
           ++KF     +K L  +   V DVDWESTF++ H P SN+    ++S +   AM +++  +
Sbjct: 65  EQKFKEMLRSKGLDTLETEVEDVDWESTFYLHHLPQSNLYDIPDMSNEYRLAM-KDFGKR 124

Query: 121 VIKIAEKLAELMCENLGLEKAHIKQLFNGTKGPSVGTKVAKYPKCPRPDLVRGLREHTDA 180
           +  +AE+L +L+CENLGLEK ++K++F+GT GP+  TK++ YP CP+P++++GLR HTDA
Sbjct: 125 LEILAEELLDLLCENLGLEKGYLKKVFHGTTGPTFATKLSNYPPCPKPEMIKGLRAHTDA 184

Query: 181 GGIILLLQDDQVPGLEFLNDGVWFKIPPSKNNAIFVNIGDQIEVLSNGKYKSILHRVLAE 240
           GG+ILL QDD+V GL+ L DG W  +PP K ++I +N+GDQ+EV++NGKYKS++HRV+ +
Sbjct: 185 GGLILLFQDDKVSGLQLLKDGDWVDVPPLK-HSIVINLGDQLEVITNGKYKSVMHRVMTQ 244

Query: 241 KDGSRLSIATFYNPAADAVISPAAELL-----YPGGYGFEDYLKFYNTTKFGDKEKRFQS 295
           K+G+R+SIA+FYNP +DA ISPA  L+     YP  + F+DY+K Y   KF  KE RF++
Sbjct: 245 KEGNRMSIASFYNPGSDAEISPATSLVDKDSKYP-SFVFDDYMKLYAGLKFQAKEPRFEA 302

BLAST of Lag0007032 vs. TAIR 10
Match: AT1G05010.1 (ethylene-forming enzyme )

HSP 1 Score: 302.0 bits (772), Expect = 5.3e-82
Identity = 152/310 (49.03%), Postives = 214/310 (69.03%), Query Frame = 0

Query: 4   PTIDFNQLTGDNRTKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHYDENMKEK 63
           P I+  +L G+ R  T+  + DAC++WGFF   NHGI  E+L+KV+ +   HY + M+E+
Sbjct: 5   PIINLEKLNGEERAITMEKIKDACENWGFFECVNHGISLELLDKVEKMTKEHYKKCMEER 64

Query: 64  FYNSDVAKNLG----KMAVSDVDWESTFFIWHKPNSNISHFTNVSQDLCKAMEEEYIPQV 123
           F  S   KN G    +  V+DVDWESTF++ H P SNIS   ++  D  + + +++  ++
Sbjct: 65  FKES--IKNRGLDSLRSEVNDVDWESTFYLKHLPVSNISDVPDLDDDY-RTLMKDFAGKI 124

Query: 124 IKIAEKLAELMCENLGLEKAHIKQLFNGTKGPSVGTKVAKYPKCPRPDLVRGLREHTDAG 183
            K++E+L +L+CENLGLEK ++K++F G+K P+ GTKV+ YP CP PDLV+GLR HTDAG
Sbjct: 125 EKLSEELLDLLCENLGLEKGYLKKVFYGSKRPTFGTKVSNYPPCPNPDLVKGLRAHTDAG 184

Query: 184 GIILLLQDDQVPGLEFLNDGVWFKIPPSKNNAIFVNIGDQIEVLSNGKYKSILHRVLAEK 243
           GIILL QDD+V GL+ L DG W  +PP K ++I VN+GDQ+EV++NGKYKS+ HRVL++ 
Sbjct: 185 GIILLFQDDKVSGLQLLKDGEWVDVPPVK-HSIVVNLGDQLEVITNGKYKSVEHRVLSQT 244

Query: 244 DG-SRLSIATFYNPAADAVISPAAELL-----------YPGGYGFEDYLKFYNTTKFGDK 298
           DG  R+SIA+FYNP +D+VI PA EL+           YP  + FEDY+K Y+  KF  K
Sbjct: 245 DGEGRMSIASFYNPGSDSVIFPAPELIGKEAEKEKKENYP-RFVFEDYMKLYSAVKFQAK 304

BLAST of Lag0007032 vs. TAIR 10
Match: AT1G77330.1 (2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein )

HSP 1 Score: 252.7 bits (644), Expect = 3.7e-67
Identity = 143/310 (46.13%), Postives = 183/310 (59.03%), Query Frame = 0

Query: 1   MKIPTIDFNQLTGDNRTKTLSVLHDACKDWGFFLVENHGIEEEVLEKVKLLVNRHYDENM 60
           M IP IDF++L G+ R KTLS +  AC++WGFF + NHGI  E+L KVK L +  Y    
Sbjct: 1   MAIPVIDFSKLNGEEREKTLSEIARACEEWGFFQLVNHGIPLELLNKVKKLSSDCYKTER 60

Query: 61  KEKFYNSDVAKNLGKMA-------VSDVDWESTFFIWHKPNSNISHFTNVSQDLCKAMEE 120
           +E F  S+  K L ++        + +VDWE  F +       + H  N      K    
Sbjct: 61  EEAFKTSNPVKLLNELVQKNSGEKLENVDWEDVFTL-------LDHNQNEWPSNIKETMG 120

Query: 121 EYIPQVIKIAEKLAELMCENLGLEKAHIKQLFN-----GTKGPSVGTKVAKYPKCPRPDL 180
           EY  +V K+A K+ E+M ENLGL K +IK+ FN     G +    GTKV+ YP CP P+L
Sbjct: 121 EYREEVRKLASKMMEVMDENLGLPKGYIKKAFNEGMEDGEETAFFGTKVSHYPPCPHPEL 180

Query: 181 VRGLREHTDAGGIILLLQDDQVPGLEFLNDGVWFKIPPSKNNAIFVNIGDQIEVLSNGKY 240
           V GLR HTDAGG++LL QDD+  GL+ L DG W  + P   NAI +N GDQIEVLSNG+Y
Sbjct: 181 VNGLRAHTDAGGVVLLFQDDEYDGLQVLKDGEWIDVQPLP-NAIVINTGDQIEVLSNGRY 240

Query: 241 KSILHRVLAEKDGSRLSIATFYNPAADAVISPAA-------ELLYPGGYGFEDYLKFYNT 292
           KS  HRVLA ++G+R SIA+FYNP+  A I PAA       E  YP  + F DY+  Y  
Sbjct: 241 KSAWHRVLAREEGNRRSIASFYNPSYKAAIGPAAVAEEEGSEKKYP-KFVFGDYMDVYAN 300

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038890644.19.6e-15086.821-aminocyclopropane-1-carboxylate oxidase 1 [Benincasa hispida][more]
XP_022138962.12.0e-14785.191-aminocyclopropane-1-carboxylate oxidase 1 [Momordica charantia][more]
XP_022965527.15.4e-14583.841-aminocyclopropane-1-carboxylate oxidase 1 [Cucurbita maxima][more]
TYK10774.11.6e-14483.671-aminocyclopropane-1-carboxylate oxidase 1 [Cucumis melo var. makuwa][more]
KAG7021341.12.7e-14483.671-aminocyclopropane-1-carboxylate oxidase 1, partial [Cucurbita argyrosperma sub... [more]
Match NameE-valueIdentityDescription
Q9ZUN49.4e-11664.311-aminocyclopropane-1-carboxylate oxidase 1 OS=Arabidopsis thaliana OX=3702 GN=A... [more]
P312371.5e-8449.351-aminocyclopropane-1-carboxylate oxidase OS=Actinidia deliciosa OX=3627 GN=ACO ... [more]
O488822.5e-8450.001-aminocyclopropane-1-carboxylate oxidase 2 OS=Malus domestica OX=3750 GN=ACO2 P... [more]
Q419314.3e-8446.841-aminocyclopropane-1-carboxylate oxidase 2 OS=Arabidopsis thaliana OX=3702 GN=A... [more]
Q009855.6e-8448.341-aminocyclopropane-1-carboxylate oxidase 1 OS=Malus domestica OX=3750 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1CCP29.7e-14885.191-aminocyclopropane-1-carboxylate oxidase 1 OS=Momordica charantia OX=3673 GN=LO... [more]
A0A6J1HKJ92.6e-14583.841-aminocyclopropane-1-carboxylate oxidase 1 OS=Cucurbita maxima OX=3661 GN=LOC11... [more]
A0A5D3CHV37.7e-14583.671-aminocyclopropane-1-carboxylate oxidase 1 OS=Cucumis melo var. makuwa OX=11946... [more]
A0A1S3CB091.7e-14483.331-aminocyclopropane-1-carboxylate oxidase 1 OS=Cucumis melo OX=3656 GN=LOC103498... [more]
A0A6J1FD263.8e-14482.831-aminocyclopropane-1-carboxylate oxidase 1 OS=Cucurbita moschata OX=3662 GN=LOC... [more]
Match NameE-valueIdentityDescription
AT2G19590.16.7e-11764.31ACC oxidase 1 [more]
AT1G62380.13.0e-8546.84ACC oxidase 2 [more]
AT1G12010.11.8e-8245.852-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
AT1G05010.15.3e-8249.03ethylene-forming enzyme [more]
AT1G77330.13.7e-6746.132-oxoglutarate (2OG) and Fe(II)-dependent oxygenase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR026992Non-haem dioxygenase N-terminal domainPFAMPF14226DIOX_Ncoord: 3..69
e-value: 3.4E-14
score: 53.6
IPR044861Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domainPFAMPF031712OG-FeII_Oxycoord: 158..252
e-value: 1.7E-23
score: 83.0
IPR027443Isopenicillin N synthase-like superfamilyGENE3D2.60.120.330coord: 1..297
e-value: 6.9E-79
score: 267.4
NoneNo IPR availablePANTHERPTHR47991:SF28BNACNNG33000D PROTEINcoord: 146..293
NoneNo IPR availablePANTHERPTHR47991OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASEcoord: 146..293
NoneNo IPR availablePANTHERPTHR47991:SF28BNACNNG33000D PROTEINcoord: 1..121
NoneNo IPR availablePANTHERPTHR47991OXOGLUTARATE/IRON-DEPENDENT DIOXYGENASEcoord: 1..121
NoneNo IPR availableSUPERFAMILY51197Clavaminate synthase-likecoord: 1..293
IPR005123Oxoglutarate/iron-dependent dioxygenasePROSITEPS51471FE2OG_OXYcoord: 149..252
score: 13.518946

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0007032.1Lag0007032.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071398 cellular response to fatty acid
biological_process GO:0071732 cellular response to nitric oxide
biological_process GO:0051365 cellular response to potassium ion starvation
biological_process GO:1901576 organic substance biosynthetic process
molecular_function GO:0051213 dioxygenase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen