Lag0006877 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0006877
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	calmodulin-like protein 7
Location	chr6: 46706960 .. 46707640 (+)
RNA-Seq Expression	Lag0006877
Synteny	Lag0006877

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAACTCCCCATCCTCAATCTCCTCTTACTCGCCGTTTTGTTCCTTGCCGGTTTGCTCAACTTCCTCTTCTATTTCCCCACCAAGCGATTCTCCTCCTGGCTCCAATCCCTAAAACCCACCTCCCAAATCCCCCATTTCAAATCCTCCCCCGCCGTCGCCGCCGTTTCCGCCTCTCCCGCCGCCGACGCGAAGGCGGAGATGAAGAGGGTGTTCGGGACCTTCGACAAGAACAGCGACGGGTTCATAACGAAGAAGGAGCTGATGGAGTCGCTGAAGAACATCAGGATGTTGGTGACAGAGGGGGAAGCAGATGAGATGTTCAGAGGGGTGGATGAGAATGGAGATGGGCTGATTGATTTTGATGAGTTCTGTGTTTTGGGGGAGAAATTGGAGATGAGGAGAGGGGTTAATGGAGAGGATGGAGATGAGGAAGATGAAGAAAAGGAAGTGCAATTGAGAGAGGCTTTTGGAGTGTTTGATAAGGATGAAGATGGGTTGATCACTGTTGAGGAATTGAGTTTGGTGCTGAATTGTTTGGGATTGAAGGAAGGGAAGAAGGTTGAGAATTGCAAGGAGATGATAAGGAAAGTGGATTTGGATGGAGATGGGATGGTGAATTTTGATGAGTTCAAGAGGATGATGAGAAGTGGAGGAGGTCTTGTTTGGGCAAAATCTTGA

mRNA sequence

ATGGAACTCCCCATCCTCAATCTCCTCTTACTCGCCGTTTTGTTCCTTGCCGGTTTGCTCAACTTCCTCTTCTATTTCCCCACCAAGCGATTCTCCTCCTGGCTCCAATCCCTAAAACCCACCTCCCAAATCCCCCATTTCAAATCCTCCCCCGCCGTCGCCGCCGTTTCCGCCTCTCCCGCCGCCGACGCGAAGGCGGAGATGAAGAGGGTGTTCGGGACCTTCGACAAGAACAGCGACGGGTTCATAACGAAGAAGGAGCTGATGGAGTCGCTGAAGAACATCAGGATGTTGGTGACAGAGGGGGAAGCAGATGAGATGTTCAGAGGGGTGGATGAGAATGGAGATGGGCTGATTGATTTTGATGAGTTCTGTGTTTTGGGGGAGAAATTGGAGATGAGGAGAGGGGTTAATGGAGAGGATGGAGATGAGGAAGATGAAGAAAAGGAAGTGCAATTGAGAGAGGCTTTTGGAGTGTTTGATAAGGATGAAGATGGGTTGATCACTGTTGAGGAATTGAGTTTGGTGCTGAATTGTTTGGGATTGAAGGAAGGGAAGAAGGTTGAGAATTGCAAGGAGATGATAAGGAAAGTGGATTTGGATGGAGATGGGATGGTGAATTTTGATGAGTTCAAGAGGATGATGAGAAGTGGAGGAGGTCTTGTTTGGGCAAAATCTTGA

Coding sequence (CDS)

ATGGAACTCCCCATCCTCAATCTCCTCTTACTCGCCGTTTTGTTCCTTGCCGGTTTGCTCAACTTCCTCTTCTATTTCCCCACCAAGCGATTCTCCTCCTGGCTCCAATCCCTAAAACCCACCTCCCAAATCCCCCATTTCAAATCCTCCCCCGCCGTCGCCGCCGTTTCCGCCTCTCCCGCCGCCGACGCGAAGGCGGAGATGAAGAGGGTGTTCGGGACCTTCGACAAGAACAGCGACGGGTTCATAACGAAGAAGGAGCTGATGGAGTCGCTGAAGAACATCAGGATGTTGGTGACAGAGGGGGAAGCAGATGAGATGTTCAGAGGGGTGGATGAGAATGGAGATGGGCTGATTGATTTTGATGAGTTCTGTGTTTTGGGGGAGAAATTGGAGATGAGGAGAGGGGTTAATGGAGAGGATGGAGATGAGGAAGATGAAGAAAAGGAAGTGCAATTGAGAGAGGCTTTTGGAGTGTTTGATAAGGATGAAGATGGGTTGATCACTGTTGAGGAATTGAGTTTGGTGCTGAATTGTTTGGGATTGAAGGAAGGGAAGAAGGTTGAGAATTGCAAGGAGATGATAAGGAAAGTGGATTTGGATGGAGATGGGATGGTGAATTTTGATGAGTTCAAGAGGATGATGAGAAGTGGAGGAGGTCTTGTTTGGGCAAAATCTTGA

Protein sequence

MELPILNLLLLAVLFLAGLLNFLFYFPTKRFSSWLQSLKPTSQIPHFKSSPAVAAVSASPAADAKAEMKRVFGTFDKNSDGFITKKELMESLKNIRMLVTEGEADEMFRGVDENGDGLIDFDEFCVLGEKLEMRRGVNGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLVLNCLGLKEGKKVENCKEMIRKVDLDGDGMVNFDEFKRMMRSGGGLVWAKS

Homology

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BLAST of Lag0006877 vs. NCBI nr
Match: KAG6599380.1 (Calmodulin-like protein 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 290.8 bits (743), Expect = 1.0e-74
Identity = 155/224 (69.20%), Postives = 186/224 (83.04%), Query Frame = 0


Query: 5   ILNLLLLAVLFLAGLLNFLFYFPTKRFSSWLQSLKPTSQIPHFKSSPAVAAVSASPAADA 64
           ++NLL LAVLF+AG +NFL YFPTKRF++W QS+KP+ Q  HFK + AV  +S +P    
Sbjct: 4   VMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPT-AVPPLSPAP-EKK 63

Query: 65  KAEMKRVFGTFDKNSDGFITKKELMESLKNIRMLVTEGEADEMFRGVDENGDGLIDFDEF 124
           K+EMK+VFGTFDKN DGFITKKELMESLKN+ M++TE EA+EM +GVDENGDGLIDF+EF
Sbjct: 64  KSEMKKVFGTFDKNKDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEEF 123

Query: 125 CVLGEKL-----EMRRGVNGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLVLNC 184
           CVLGEKL     E +R V  ED DE+++E    LREAFGVFDKD+DG+I+VEEL LVL+C
Sbjct: 124 CVLGEKLVMGFEEKQRRVEDEDEDEDEDE----LREAFGVFDKDKDGMISVEELGLVLSC 183

Query: 185 LGLKEGKKVENCKEMIRKVDLDGDGMVNFDEFKRMMRSGGGLVW 224
           LG+ EGKK+ENCKEMI+KVDLDGDGMVNFDEFKRMMRSG  LV+
Sbjct: 184 LGMNEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVF 221

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BLAST of Lag0006877 vs. NCBI nr
Match: XP_022946838.1 (calmodulin-like protein 7 [Cucurbita moschata])

HSP 1 Score: 290.4 bits (742), Expect = 1.3e-74
Identity = 155/224 (69.20%), Postives = 186/224 (83.04%), Query Frame = 0


Query: 5   ILNLLLLAVLFLAGLLNFLFYFPTKRFSSWLQSLKPTSQIPHFKSSPAVAAVSASPAADA 64
           ++NLL LAVLF+AG +NFL YFPTKRF++W QS+KP+ Q  HFK + AV  +S +P    
Sbjct: 4   VMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPT-AVPPLSPAP-EKK 63

Query: 65  KAEMKRVFGTFDKNSDGFITKKELMESLKNIRMLVTEGEADEMFRGVDENGDGLIDFDEF 124
           K+EMK+VFGTFDKN DGFITKKELMESLKN+ M++TE EA+EM +GVDENGDGLIDF+EF
Sbjct: 64  KSEMKKVFGTFDKNKDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEEF 123

Query: 125 CVLGEKL-----EMRRGVNGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLVLNC 184
           CVLGEKL     E +R V  ED DE+++E    LREAFGVFDKD+DG+I+VEEL LVL+C
Sbjct: 124 CVLGEKLVMGFEEKQRRVEDEDEDEDEDE----LREAFGVFDKDKDGVISVEELGLVLSC 183

Query: 185 LGLKEGKKVENCKEMIRKVDLDGDGMVNFDEFKRMMRSGGGLVW 224
           LG+ EGKK+ENCKEMI+KVDLDGDGMVNFDEFKRMMRSG  LV+
Sbjct: 184 LGMNEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVF 221

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BLAST of Lag0006877 vs. NCBI nr
Match: XP_038889113.1 (calmodulin-like protein 3 [Benincasa hispida])

HSP 1 Score: 289.7 bits (740), Expect = 2.2e-74
Identity = 156/223 (69.96%), Postives = 182/223 (81.61%), Query Frame = 0


Query: 4   PILNLLLLAVLFLAGLLNFLFYFPTKRFSSWLQSLKPTSQIPHFKSSPAVAAVSASPAAD 63
           PI+N LLL VL +AG +NFL YFPTKRFS+W QS+KP+ QI HFKS  ++      P + 
Sbjct: 3   PIMNFLLLVVLVVAGFVNFLLYFPTKRFSAWFQSIKPSPQISHFKSL-SLQPPPPPPPSP 62

Query: 64  AKAEMKRVFGTFDKNSDGFITKKELMESLKNIRMLVTEGEADEMFRGVDENGDGLIDFDE 123
              E+K+VFGTFDKN+DGFITKKELMESLKN+RML+TE +A+EM +GVDENGDGLIDF+E
Sbjct: 63  PSPELKKVFGTFDKNNDGFITKKELMESLKNMRMLITEKDAEEMLKGVDENGDGLIDFEE 122

Query: 124 FCVLGEKLEMRRGVNGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLVLNCLGLK 183
           FCVLGEKL M  G   +    EDEE E +LREAFGVFDKD+DG+I+VEELSLVL  LG+ 
Sbjct: 123 FCVLGEKLVMGFG-EKKKICVEDEEDEDELREAFGVFDKDKDGMISVEELSLVLCSLGMN 182

Query: 184 EGKKVENCKEMIRKVDLDGDGMVNFDEFKRMMRSGGGLVWAKS 227
           EGKKVENCKEMI+KVDLDGDGMVNFDEFKRMMRSG  LV A+S
Sbjct: 183 EGKKVENCKEMIKKVDLDGDGMVNFDEFKRMMRSGVSLVLAQS 223

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BLAST of Lag0006877 vs. NCBI nr
Match: XP_023545557.1 (calmodulin-like protein 7 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 288.9 bits (738), Expect = 3.8e-74
Identity = 152/219 (69.41%), Postives = 186/219 (84.93%), Query Frame = 0


Query: 5   ILNLLLLAVLFLAGLLNFLFYFPTKRFSSWLQSLKPTSQIPHFKSSPAVAAVSASPAADA 64
           ++NLL LAVLF+AG +NFL YFPTKRF++W QS+KP+ Q  HFK + AV  +S +P    
Sbjct: 4   VMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPT-AVPPLSPAP-EKK 63

Query: 65  KAEMKRVFGTFDKNSDGFITKKELMESLKNIRMLVTEGEADEMFRGVDENGDGLIDFDEF 124
           K+EMK+VFGTFDKN+DGFITKKELMESLKN+ M++TE EA+EM +GVDENGDGLIDF+EF
Sbjct: 64  KSEMKKVFGTFDKNNDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEEF 123

Query: 125 CVLGEKLEMRRGVNGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLVLNCLGLKE 184
           CVLGEKL M   V     ++EDE+++ +LREAFGVFDKD+DG+I+VEEL LVL+CLG+ E
Sbjct: 124 CVLGEKLVMGFEVKQRRVEDEDEDED-ELREAFGVFDKDKDGVISVEELGLVLSCLGMNE 183

Query: 185 GKKVENCKEMIRKVDLDGDGMVNFDEFKRMMRSGGGLVW 224
           GKK+ENCKEMI+KVDLDGDGMVNFDEFKRMMRSG  LV+
Sbjct: 184 GKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVF 219

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BLAST of Lag0006877 vs. NCBI nr
Match: XP_022999398.1 (calmodulin-like protein 7 [Cucurbita maxima])

HSP 1 Score: 285.8 bits (730), Expect = 3.2e-73
Identity = 153/225 (68.00%), Postives = 182/225 (80.89%), Query Frame = 0


Query: 5   ILNLLLLAVLFLAGLLNFLFYFPTKRFSSWLQSLKPTSQIPHFKSSPAVAAVSASPAAD- 64
           ++NLL LAVLF+AG +NFL YFPTKRF++W QS+KP+ Q  HFK +   A    SPA + 
Sbjct: 4   VMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPT---AVPPPSPAPEK 63

Query: 65  AKAEMKRVFGTFDKNSDGFITKKELMESLKNIRMLVTEGEADEMFRGVDENGDGLIDFDE 124
            K+EMK+VFGTFDKN+DGFITKKELMESLKN+ M++TE EA+EM +GVDENGDGLIDF+E
Sbjct: 64  KKSEMKKVFGTFDKNNDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE 123

Query: 125 FCVLGEKL-----EMRRGVNGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLVLN 184
           FCVLGEKL     E +R +  ED DE        LREAFGVFDKD+DG+I+VEEL LVL+
Sbjct: 124 FCVLGEKLVVGFEEKQRRIEDEDEDE--------LREAFGVFDKDKDGVISVEELGLVLS 183

Query: 185 CLGLKEGKKVENCKEMIRKVDLDGDGMVNFDEFKRMMRSGGGLVW 224
           CLG+ EGKK+ENCKEMI+KVDLDGDGMVNFDEFKRMMRSG  LV+
Sbjct: 184 CLGMNEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVF 217

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BLAST of Lag0006877 vs. ExPASy Swiss-Prot
Match: Q9LNE7 (Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 1.1e-34
Identity = 77/153 (50.33%), Postives = 108/153 (70.59%), Query Frame = 0


Query: 67  EMKRVFGTFDKNSDGFITKKELMESLKNIRMLVTEGEADEMFRGVDENGDGLIDFDEFCV 126
           E+KRVF  FDKN DG IT KEL E+L+++ + + + E  +M   +D NGDG +D DEF  
Sbjct: 5   ELKRVFQMFDKNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDIDEFGE 64

Query: 127 LGEKLEMRRGVNGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLVLNCLGLKEGK 186
           L + +           DEEDEE+E  ++EAF VFD++ DG ITV+EL  VL+ LGLK+GK
Sbjct: 65  LYKTIM----------DEEDEEEE-DMKEAFNVFDQNGDGFITVDELKAVLSSLGLKQGK 124

Query: 187 KVENCKEMIRKVDLDGDGMVNFDEFKRMMRSGG 220
            +++CK+MI+KVD+DGDG VN+ EF++MM+ GG
Sbjct: 125 TLDDCKKMIKKVDVDGDGRVNYKEFRQMMKGGG 146

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BLAST of Lag0006877 vs. ExPASy Swiss-Prot
Match: Q9SU00 (Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1)

HSP 1 Score: 145.2 bits (365), Expect = 9.0e-34
Identity = 77/155 (49.68%), Postives = 104/155 (67.10%), Query Frame = 0


Query: 65  KAEMKRVFGTFDKNSDGFITKKELMESLKNIRMLVTEGEADEMFRGVDENGDGLIDFDEF 124
           + E+ RVF  FDKN DG I K EL +  K++ ++V E E +EM   +D NGDG +D DEF
Sbjct: 3   RGELSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEF 62

Query: 125 CVLGEKLEMRRGVNGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLVLNCLGLKE 184
             L +++             E++E+E  +REAF VFD++ DG IT EEL  VL  +GLK+
Sbjct: 63  GSLYQEM------------VEEKEEEEDMREAFRVFDQNGDGFITDEELRSVLASMGLKQ 122

Query: 185 GKKVENCKEMIRKVDLDGDGMVNFDEFKRMMRSGG 220
           G+ +E+CK+MI KVD+DGDGMVNF EFK+MMR GG
Sbjct: 123 GRTLEDCKKMISKVDVDGDGMVNFKEFKQMMRGGG 145

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BLAST of Lag0006877 vs. ExPASy Swiss-Prot
Match: Q9SRR7 (Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1)

HSP 1 Score: 143.7 bits (361), Expect = 2.6e-33
Identity = 76/155 (49.03%), Postives = 107/155 (69.03%), Query Frame = 0


Query: 65  KAEMKRVFGTFDKNSDGFITKKELMESLKNIRMLVTEGEADEMFRGVDENGDGLIDFDEF 124
           +AE+ R+F  FD+N DG ITK+EL +SL+N+ + + + +  +M   +D NGDG +D +EF
Sbjct: 3   QAELARIFQMFDRNGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEF 62

Query: 125 CVLGEKLEMRRGVNGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLVLNCLGLKE 184
             L + +           +E DEE++  +REAF VFD++ DG ITVEEL  VL  LGLK+
Sbjct: 63  GGLYQTIM----------EERDEEED--MREAFNVFDQNRDGFITVEELRSVLASLGLKQ 122

Query: 185 GKKVENCKEMIRKVDLDGDGMVNFDEFKRMMRSGG 220
           G+ +E+CK MI KVD+DGDGMVNF EFK+MM+ GG
Sbjct: 123 GRTLEDCKRMISKVDVDGDGMVNFKEFKQMMKGGG 145

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BLAST of Lag0006877 vs. ExPASy Swiss-Prot
Match: Q9ZR02 (Calmodulin-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=CML6 PE=2 SV=1)

HSP 1 Score: 139.8 bits (351), Expect = 3.8e-32
Identity = 71/152 (46.71%), Postives = 104/152 (68.42%), Query Frame = 0


Query: 67  EMKRVFGTFDKNSDGFITKKELMESLKNIRMLVTEGEADEMFRGVDENGDGLIDFDEFCV 126
           E+ RVF  FDK+ DG IT KEL ES KN+ +++ E E  ++ + +D NGDG +D +EF  
Sbjct: 5   ELNRVFQMFDKDGDGKITTKELNESFKNLGIIIPEDELTQIIQKIDVNGDGCVDIEEFGE 64

Query: 127 LGEKLEMRRGVNGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLVLNCLGLKEGK 186
           L + + +         ++EDE  E  ++EAF VFD++ DG ITV+EL  VL+ LGLK+GK
Sbjct: 65  LYKTIMV---------EDEDEVGEEDMKEAFNVFDRNGDGFITVDELKAVLSSLGLKQGK 124

Query: 187 KVENCKEMIRKVDLDGDGMVNFDEFKRMMRSG 219
            +E C++MI +VD+DGDG VN+ EF++MM+ G
Sbjct: 125 TLEECRKMIMQVDVDGDGRVNYMEFRQMMKKG 147

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BLAST of Lag0006877 vs. ExPASy Swiss-Prot
Match: Q2QVI1 (Probable calcium-binding protein CML28 OS=Oryza sativa subsp. japonica OX=39947 GN=CML28 PE=2 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 6.4e-32
Identity = 77/164 (46.95%), Postives = 103/164 (62.80%), Query Frame = 0


Query: 67  EMKRVFGTFDKNSDGFITKKELMESLKNIRMLVTEGEADEMFRGVDENGDGLIDFDEF-- 126
           E+++VF  FDKN DG ITKKEL ES KN  + + + E D     +D NGDG +D +EF  
Sbjct: 5   ELRKVFKMFDKNGDGRITKKELGESFKNFGIFIPDDELDATMDKIDANGDGCVDVEEFGL 64

Query: 127 ---CVLGEKLEMR------RGVNGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSL 186
               +LG+    R        + GE G  +DE++   +REAF VFD++ DG ITV+EL  
Sbjct: 65  LYRSILGDDAAGRAPRTAAAAIGGEGGAPDDEDE--GMREAFNVFDQNGDGFITVDELRS 124

Query: 187 VLNCLGLKEGKKVENCKEMIRKVDLDGDGMVNFDEFKRMMRSGG 220
           VL+ LGLK G+  ++C+ MI  VD DGDG V+F EFK+MMR GG
Sbjct: 125 VLSSLGLKHGRTADDCRRMISMVDADGDGRVDFKEFKQMMRGGG 166

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BLAST of Lag0006877 vs. ExPASy TrEMBL
Match: A0A6J1G4Z1 (calmodulin-like protein 7 OS=Cucurbita moschata OX=3662 GN=LOC111450782 PE=4 SV=1)

HSP 1 Score: 290.4 bits (742), Expect = 6.4e-75
Identity = 155/224 (69.20%), Postives = 186/224 (83.04%), Query Frame = 0


Query: 5   ILNLLLLAVLFLAGLLNFLFYFPTKRFSSWLQSLKPTSQIPHFKSSPAVAAVSASPAADA 64
           ++NLL LAVLF+AG +NFL YFPTKRF++W QS+KP+ Q  HFK + AV  +S +P    
Sbjct: 4   VMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPT-AVPPLSPAP-EKK 63

Query: 65  KAEMKRVFGTFDKNSDGFITKKELMESLKNIRMLVTEGEADEMFRGVDENGDGLIDFDEF 124
           K+EMK+VFGTFDKN DGFITKKELMESLKN+ M++TE EA+EM +GVDENGDGLIDF+EF
Sbjct: 64  KSEMKKVFGTFDKNKDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEEF 123

Query: 125 CVLGEKL-----EMRRGVNGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLVLNC 184
           CVLGEKL     E +R V  ED DE+++E    LREAFGVFDKD+DG+I+VEEL LVL+C
Sbjct: 124 CVLGEKLVMGFEEKQRRVEDEDEDEDEDE----LREAFGVFDKDKDGVISVEELGLVLSC 183

Query: 185 LGLKEGKKVENCKEMIRKVDLDGDGMVNFDEFKRMMRSGGGLVW 224
           LG+ EGKK+ENCKEMI+KVDLDGDGMVNFDEFKRMMRSG  LV+
Sbjct: 184 LGMNEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVF 221

[Close]

BLAST of Lag0006877 vs. ExPASy TrEMBL
Match: A0A6J1KJL5 (calmodulin-like protein 7 OS=Cucurbita maxima OX=3661 GN=LOC111493780 PE=4 SV=1)

HSP 1 Score: 285.8 bits (730), Expect = 1.6e-73
Identity = 153/225 (68.00%), Postives = 182/225 (80.89%), Query Frame = 0


Query: 5   ILNLLLLAVLFLAGLLNFLFYFPTKRFSSWLQSLKPTSQIPHFKSSPAVAAVSASPAAD- 64
           ++NLL LAVLF+AG +NFL YFPTKRF++W QS+KP+ Q  HFK +   A    SPA + 
Sbjct: 4   VMNLLFLAVLFVAGFVNFLMYFPTKRFTAWFQSIKPSPQSVHFKPT---AVPPPSPAPEK 63

Query: 65  AKAEMKRVFGTFDKNSDGFITKKELMESLKNIRMLVTEGEADEMFRGVDENGDGLIDFDE 124
            K+EMK+VFGTFDKN+DGFITKKELMESLKN+ M++TE EA+EM +GVDENGDGLIDF+E
Sbjct: 64  KKSEMKKVFGTFDKNNDGFITKKELMESLKNMSMMITEREAEEMVKGVDENGDGLIDFEE 123

Query: 125 FCVLGEKL-----EMRRGVNGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLVLN 184
           FCVLGEKL     E +R +  ED DE        LREAFGVFDKD+DG+I+VEEL LVL+
Sbjct: 124 FCVLGEKLVVGFEEKQRRIEDEDEDE--------LREAFGVFDKDKDGVISVEELGLVLS 183

Query: 185 CLGLKEGKKVENCKEMIRKVDLDGDGMVNFDEFKRMMRSGGGLVW 224
           CLG+ EGKK+ENCKEMI+KVDLDGDGMVNFDEFKRMMRSG  LV+
Sbjct: 184 CLGMNEGKKLENCKEMIKKVDLDGDGMVNFDEFKRMMRSGTTLVF 217

[Close]

BLAST of Lag0006877 vs. ExPASy TrEMBL
Match: A0A0A0LL19 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G007990 PE=4 SV=1)

HSP 1 Score: 279.3 bits (713), Expect = 1.5e-71
Identity = 148/221 (66.97%), Postives = 178/221 (80.54%), Query Frame = 0


Query: 4   PILNLLLLAVLFLAGLLNFLFYFPTKRFSSWLQSLKPTSQIPHFKSS------PAVAAVS 63
           PI N LLL+VLF+AG +NFL YFP+KRFS+W QS+KP+SQI HFKS+      P   + S
Sbjct: 3   PIFNFLLLSVLFVAGFINFLLYFPSKRFSAWFQSIKPSSQITHFKSTPLQPPPPPSPSPS 62

Query: 64  ASPAADAKAEMKRVFGTFDKNSDGFITKKELMESLKNIRMLVTEGEADEMFRGVDENGDG 123
            SP   +  EMK+VFGTFDKN DGFITKKELMESLK++RM++TE +A+EM +GVDENGDG
Sbjct: 63  PSPPPPSAMEMKKVFGTFDKNDDGFITKKELMESLKSMRMMITEKDAEEMLKGVDENGDG 122

Query: 124 LIDFDEFCVLGEKLEMRRGVNGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLVL 183
           LIDF+EFCVLG KL M    N +   E++E+   +L++AFGVFDKD DGLI+VEELSLVL
Sbjct: 123 LIDFEEFCVLGGKLMMGFEENKKTSVEDEED---ELKDAFGVFDKDSDGLISVEELSLVL 182

Query: 184 NCLGLKEGKKVENCKEMIRKVDLDGDGMVNFDEFKRMMRSG 219
             LG+ EGK VENCKEMIRKVDLDGDGMVNFDEFK+MMR+G
Sbjct: 183 CSLGMNEGKIVENCKEMIRKVDLDGDGMVNFDEFKKMMRNG 220

[Close]

BLAST of Lag0006877 vs. ExPASy TrEMBL
Match: A0A1S3C5X4 (calmodulin-like protein 7 OS=Cucumis melo OX=3656 GN=LOC103496824 PE=4 SV=1)

HSP 1 Score: 275.0 bits (702), Expect = 2.8e-70
Identity = 147/219 (67.12%), Postives = 174/219 (79.45%), Query Frame = 0


Query: 4   PILNLLLLAVLFLAGLLNFLFYFPTKRFSSWLQSLKPTSQIPHFKSS----PAVAAVSAS 63
           PI N LLL+VLF+AG +NFL YFPTKRF++W QS+KP+SQIPHFKS+    P        
Sbjct: 3   PIFNFLLLSVLFVAGFVNFLLYFPTKRFTAWFQSIKPSSQIPHFKSTPLQPPPPPPPPPP 62

Query: 64  PAADAKAEMKRVFGTFDKNSDGFITKKELMESLKNIRMLVTEGEADEMFRGVDENGDGLI 123
           P   +  E+K+VFGTFDKN DGFITKKELMESLK++RM++TE +A+EM + VDENGDGLI
Sbjct: 63  PPPPSAMELKKVFGTFDKNDDGFITKKELMESLKSMRMMITEKDAEEMLKEVDENGDGLI 122

Query: 124 DFDEFCVLGEKLEMRRGVNGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLVLNC 183
           DF+EFCVLGEKL M    N +    +DEE    L++AFGVFDKD DGLI+VEELSLVL  
Sbjct: 123 DFEEFCVLGEKLLMGFEENKKTSVGDDEE---GLKDAFGVFDKDSDGLISVEELSLVLCS 182

Query: 184 LGLKEGKKVENCKEMIRKVDLDGDGMVNFDEFKRMMRSG 219
           LG+ EGK VENCKEMIRKVDLDGDGMVNFDEFK+MMR+G
Sbjct: 183 LGMNEGKIVENCKEMIRKVDLDGDGMVNFDEFKKMMRNG 218

[Close]

BLAST of Lag0006877 vs. ExPASy TrEMBL
Match: A0A6J1CMI5 (calmodulin-like protein 7 OS=Momordica charantia OX=3673 GN=LOC111012994 PE=4 SV=1)

HSP 1 Score: 246.1 bits (627), Expect = 1.4e-61
Identity = 133/215 (61.86%), Postives = 168/215 (78.14%), Query Frame = 0


Query: 5   ILNLLLLAVLFLAGLLNFLFYFPTKRFSSWLQSLKPTSQIPHFKSSPAVAAVSASPAADA 64
           ++N+LLLAVLF+AGL+NFL YFPTKRFS+WL+   PT         PA  A + +P    
Sbjct: 69  MMNILLLAVLFVAGLVNFLVYFPTKRFSAWLKQ-SPT--------KPAAVAAAVAP-PQP 128

Query: 65  KAEMKRVFGTFDKNSDGFITKKELMESLKNIRMLVTEGEADEMFRGVDENGDGLIDFDEF 124
             E++++F TFDKN+DG+IT+KEL ESL+N    + E EA+E+F GVDENGDGLIDF+EF
Sbjct: 129 PTELRKIFRTFDKNNDGYITRKELTESLRN---WIAEREAEELFEGVDENGDGLIDFEEF 188

Query: 125 CVLGEKLEMRRGVNGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLVLNCLGLKE 184
           CVL EKL        ++ +EE+E+ + +LREAFGVFDKD+DG+I+VEEL LVL+CLGLKE
Sbjct: 189 CVLAEKLR-------KEKEEEEEDDDEELREAFGVFDKDKDGVISVEELGLVLSCLGLKE 248

Query: 185 GKKVENCKEMIRKVDLDGDGMVNFDEFKRMMRSGG 220
           GK  ENC+EMIRKVDLDGDGMVNF+EFKRMMR+GG
Sbjct: 249 GKMAENCREMIRKVDLDGDGMVNFEEFKRMMRNGG 263

[Close]

BLAST of Lag0006877 vs. TAIR 10
Match: AT1G05990.1 (EF hand calcium-binding protein family )

HSP 1 Score: 148.3 bits (373), Expect = 7.5e-36
Identity = 77/153 (50.33%), Postives = 108/153 (70.59%), Query Frame = 0


Query: 67  EMKRVFGTFDKNSDGFITKKELMESLKNIRMLVTEGEADEMFRGVDENGDGLIDFDEFCV 126
           E+KRVF  FDKN DG IT KEL E+L+++ + + + E  +M   +D NGDG +D DEF  
Sbjct: 5   ELKRVFQMFDKNGDGTITGKELSETLRSLGIYIPDKELTQMIEKIDVNGDGCVDIDEFGE 64

Query: 127 LGEKLEMRRGVNGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLVLNCLGLKEGK 186
           L + +           DEEDEE+E  ++EAF VFD++ DG ITV+EL  VL+ LGLK+GK
Sbjct: 65  LYKTIM----------DEEDEEEE-DMKEAFNVFDQNGDGFITVDELKAVLSSLGLKQGK 124

Query: 187 KVENCKEMIRKVDLDGDGMVNFDEFKRMMRSGG 220
            +++CK+MI+KVD+DGDG VN+ EF++MM+ GG
Sbjct: 125 TLDDCKKMIKKVDVDGDGRVNYKEFRQMMKGGG 146

[Close]

BLAST of Lag0006877 vs. TAIR 10
Match: AT4G12860.1 (EF hand calcium-binding protein family )

HSP 1 Score: 145.2 bits (365), Expect = 6.4e-35
Identity = 77/155 (49.68%), Postives = 104/155 (67.10%), Query Frame = 0


Query: 65  KAEMKRVFGTFDKNSDGFITKKELMESLKNIRMLVTEGEADEMFRGVDENGDGLIDFDEF 124
           + E+ RVF  FDKN DG I K EL +  K++ ++V E E +EM   +D NGDG +D DEF
Sbjct: 3   RGELSRVFQMFDKNGDGKIAKNELKDFFKSVGIMVPENEINEMIAKMDVNGDGAMDIDEF 62

Query: 125 CVLGEKLEMRRGVNGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLVLNCLGLKE 184
             L +++             E++E+E  +REAF VFD++ DG IT EEL  VL  +GLK+
Sbjct: 63  GSLYQEM------------VEEKEEEEDMREAFRVFDQNGDGFITDEELRSVLASMGLKQ 122

Query: 185 GKKVENCKEMIRKVDLDGDGMVNFDEFKRMMRSGG 220
           G+ +E+CK+MI KVD+DGDGMVNF EFK+MMR GG
Sbjct: 123 GRTLEDCKKMISKVDVDGDGMVNFKEFKQMMRGGG 145

[Close]

BLAST of Lag0006877 vs. TAIR 10
Match: AT3G07490.1 (ARF-GAP domain 11 )

HSP 1 Score: 143.7 bits (361), Expect = 1.9e-34
Identity = 76/155 (49.03%), Postives = 107/155 (69.03%), Query Frame = 0


Query: 65  KAEMKRVFGTFDKNSDGFITKKELMESLKNIRMLVTEGEADEMFRGVDENGDGLIDFDEF 124
           +AE+ R+F  FD+N DG ITK+EL +SL+N+ + + + +  +M   +D NGDG +D +EF
Sbjct: 3   QAELARIFQMFDRNGDGKITKQELNDSLENLGIYIPDKDLVQMIEKIDLNGDGYVDIEEF 62

Query: 125 CVLGEKLEMRRGVNGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLVLNCLGLKE 184
             L + +           +E DEE++  +REAF VFD++ DG ITVEEL  VL  LGLK+
Sbjct: 63  GGLYQTIM----------EERDEEED--MREAFNVFDQNRDGFITVEELRSVLASLGLKQ 122

Query: 185 GKKVENCKEMIRKVDLDGDGMVNFDEFKRMMRSGG 220
           G+ +E+CK MI KVD+DGDGMVNF EFK+MM+ GG
Sbjct: 123 GRTLEDCKRMISKVDVDGDGMVNFKEFKQMMKGGG 145

[Close]

BLAST of Lag0006877 vs. TAIR 10
Match: AT4G03290.1 (EF hand calcium-binding protein family )

HSP 1 Score: 139.8 bits (351), Expect = 2.7e-33
Identity = 71/152 (46.71%), Postives = 104/152 (68.42%), Query Frame = 0


Query: 67  EMKRVFGTFDKNSDGFITKKELMESLKNIRMLVTEGEADEMFRGVDENGDGLIDFDEFCV 126
           E+ RVF  FDK+ DG IT KEL ES KN+ +++ E E  ++ + +D NGDG +D +EF  
Sbjct: 5   ELNRVFQMFDKDGDGKITTKELNESFKNLGIIIPEDELTQIIQKIDVNGDGCVDIEEFGE 64

Query: 127 LGEKLEMRRGVNGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLVLNCLGLKEGK 186
           L + + +         ++EDE  E  ++EAF VFD++ DG ITV+EL  VL+ LGLK+GK
Sbjct: 65  LYKTIMV---------EDEDEVGEEDMKEAFNVFDRNGDGFITVDELKAVLSSLGLKQGK 124

Query: 187 KVENCKEMIRKVDLDGDGMVNFDEFKRMMRSG 219
            +E C++MI +VD+DGDG VN+ EF++MM+ G
Sbjct: 125 TLEECRKMIMQVDVDGDGRVNYMEFRQMMKKG 147

[Close]

BLAST of Lag0006877 vs. TAIR 10
Match: AT2G43290.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 137.5 bits (345), Expect = 1.3e-32
Identity = 89/221 (40.27%), Postives = 133/221 (60.18%), Query Frame = 0


Query: 10  LLAVLFLAGLLN-FLFYFPTKRFSSWLQSLKPTS---QIPHFKSSPAVAAVSASPAADA- 69
           ++ +  L  +LN FL     K+    L++L P S   +  H  S P+ + +  SP++ + 
Sbjct: 1   MVRIFLLYNILNSFLLSLVPKK----LRTLFPLSWFDKTLHKNSPPSPSTMLPSPSSSSA 60

Query: 70  ------KAEMKRVFGTFDKNSDGFITKKELMESLKNIRMLVTEGEADEMFRGVDENGDGL 129
                  +E+KRVF  FDKN DG ITK+EL +SL+N+ + + + +  +M   +D NGDG 
Sbjct: 61  PTKRIDPSELKRVFQMFDKNGDGRITKEELNDSLENLGIYIPDKDLTQMIHKIDANGDGC 120

Query: 130 IDFDEFCVLGEKLEMRRGVNGEDGDEEDEEKEVQLREAFGVFDKDEDGLITVEELSLVLN 189
           +D DEF  L   +     V+    D E EE++  +++AF VFD+D DG ITVEEL  V+ 
Sbjct: 121 VDIDEFESLYSSI-----VDEHHNDGETEEED--MKDAFNVFDQDGDGFITVEELKSVMA 180

Query: 190 CLGLKEGKKVENCKEMIRKVDLDGDGMVNFDEFKRMMRSGG 220
            LGLK+GK ++ CK+MI +VD DGDG VN+ EF +MM+ GG
Sbjct: 181 SLGLKQGKTLDGCKKMIMQVDADGDGRVNYKEFLQMMKGGG 210

The following BLAST results are available for this feature:

BLAST of Lag0006877 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6599380.1	1.0e-74	69.20	Calmodulin-like protein 4, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022946838.1	1.3e-74	69.20	calmodulin-like protein 7 [Cucurbita moschata]	[more]
XP_038889113.1	2.2e-74	69.96	calmodulin-like protein 3 [Benincasa hispida]	[more]
XP_023545557.1	3.8e-74	69.41	calmodulin-like protein 7 [Cucurbita pepo subsp. pepo]	[more]
XP_022999398.1	3.2e-73	68.00	calmodulin-like protein 7 [Cucurbita maxima]	[more]

BLAST of Lag0006877 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LNE7	1.1e-34	50.33	Calmodulin-like protein 7 OS=Arabidopsis thaliana OX=3702 GN=CML7 PE=2 SV=1	[more]
Q9SU00	9.0e-34	49.68	Calmodulin-like protein 2 OS=Arabidopsis thaliana OX=3702 GN=CML2 PE=2 SV=1	[more]
Q9SRR7	2.6e-33	49.03	Calmodulin-like protein 3 OS=Arabidopsis thaliana OX=3702 GN=CML3 PE=2 SV=1	[more]
Q9ZR02	3.8e-32	46.71	Calmodulin-like protein 6 OS=Arabidopsis thaliana OX=3702 GN=CML6 PE=2 SV=1	[more]
Q2QVI1	6.4e-32	46.95	Probable calcium-binding protein CML28 OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of Lag0006877 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G4Z1	6.4e-75	69.20	calmodulin-like protein 7 OS=Cucurbita moschata OX=3662 GN=LOC111450782 PE=4 SV=...	[more]
A0A6J1KJL5	1.6e-73	68.00	calmodulin-like protein 7 OS=Cucurbita maxima OX=3661 GN=LOC111493780 PE=4 SV=1	[more]
A0A0A0LL19	1.5e-71	66.97	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G007990 PE=4 SV=1	[more]
A0A1S3C5X4	2.8e-70	67.12	calmodulin-like protein 7 OS=Cucumis melo OX=3656 GN=LOC103496824 PE=4 SV=1	[more]
A0A6J1CMI5	1.4e-61	61.86	calmodulin-like protein 7 OS=Momordica charantia OX=3673 GN=LOC111012994 PE=4 SV...	[more]

BLAST of Lag0006877 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G05990.1	7.5e-36	50.33	EF hand calcium-binding protein family	[more]
AT4G12860.1	6.4e-35	49.68	EF hand calcium-binding protein family	[more]
AT3G07490.1	1.9e-34	49.03	ARF-GAP domain 11	[more]
AT4G03290.1	2.7e-33	46.71	EF hand calcium-binding protein family	[more]
AT2G43290.1	1.3e-32	40.27	Calcium-binding EF-hand family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 103..131 e-value: 1.5E-5 score: 34.5 coord: 152..180 e-value: 0.0058 score: 25.8 coord: 67..95 e-value: 8.5E-4 score: 28.6 coord: 190..218 e-value: 3.3E-6 score: 36.6
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 152..216 e-value: 1.5E-12 score: 47.8
IPR002048	EF-hand domain	PFAM	PF13833	EF-hand_8	coord: 79..127 e-value: 2.0E-12 score: 46.7
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 99..134 score: 12.588623
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 148..183 score: 13.843916
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 186..221 score: 14.931837
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 63..98 score: 12.588623
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 67..125 e-value: 3.2159E-15 score: 65.6469
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 152..216 e-value: 1.90533E-16 score: 69.1137
None	No IPR available	GENE3D	1.10.238.10		coord: 149..217 e-value: 1.2E-20 score: 75.5
None	No IPR available	GENE3D	1.10.238.10		coord: 30..131 e-value: 1.2E-19 score: 72.1
None	No IPR available	PANTHER	PTHR10891	EF-HAND CALCIUM-BINDING DOMAIN CONTAINING PROTEIN	coord: 6..222
None	No IPR available	PANTHER	PTHR10891:SF796	CALCIUM-BINDING PROTEIN CML30-RELATED	coord: 6..222
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 161..173
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 112..124
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 199..211
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 76..88
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 64..217

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0006877.1	Lag0006877.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0005509	calcium ion binding