Lag0006652 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0006652
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionDisease resistance protein RPP4-like
Locationchr6: 44549768 .. 44551644 (+)
RNA-Seq ExpressionLag0006652
SyntenyLag0006652
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAATCCAAGGGACAGTTAGTTTTACCAGTCTTCTACAAGGTGGATCCATCCGAAGTACGAAAACAAACTGGTAGATTTGGTGAAGCATTGGCCAAACATGAAGCTAAGTTTATGAGCACCAAGAAAATTCAAACATGGAAGGAAGCTTTGACTATTGCTGCTGATTTGTCTGGTTGGAATTTAGGAACTAGGTATGTTTTTGTGGAGATTTTAGTTAATGTCTTTTCCTTTCTAGATTTCATTTTCGTCGGTTCACCATTTTTTTTTCATATTAACGAGCCCACCAATAATAAAAATGACTAATTCCAATTTTTCTTGTATGATGTACTTAACAACAGAAAGGAGGCTGATCTAATTGAAGATCTTGTTAAAGAAGTGTTGTCGGTATTAAATCGCACTAGCACACCATTATATGTAGCTAAGTATCCAGTTGGACTTGACTCTCAATTATTAGGACAGTTGAATTTTCACTCCCAATATGGGACACAACATTTGTCTACTGGTGTTTACATGTTGGGGATTTATGGCATTGGAGGTATTGGCAAGACAACTTTCGCCAAAGCTTTATTCAACAAAATTGCTCATCAATTTGAAGGTTCTTGTTTTCTATTTAATGTTAGAGAAACTTCAAAGCAGTTCAATGGCCTAATTCAACTACAAGAAAAGCTACTCTATGAAATCTTAAAGGATGATTCGAAGATTGTCAATCTCGACGAAGGAATTAGTATCATAAGTAATAGACTGTGTTCAAAGAAAGTTCTTATAGTTCTTGATGATGTGGATAAGCTTGGACAATTACAAGCATTGGTTGGTGGTCGTGATTGGTTTGGTCGAGATAGTAAAATCATTGTGACAACAAGGTATAAACTAATAATGTATATGGTTTTGGTATTTGTTAGTTTGTTATTAATTAGTTTTACTTTAGTTTCTCATTCTCCAGTGTACTTCTCCTTGTATAGGGTATAAATACCCAAGTCTGTTTTCATTAATGATATGAGGAGATTTACCATATTGTTGTAAATCTTTATAAACATTTACTTTCTAGCCATGGATTTGATCAAATGCATGATATTCAAGGATTGAATCCTAAAAACGCTTTGGAGTTGTTTAGTTGGCATGCTTTTAAGAAAAGTCAACCATTAAGTAATTATTTAGACCTTTCAAAATGTGCTACGACTTATTGTAAAGGTCTTCCTTTGGCTCTTGTTGTTTTGGGTTCTTTCCTTTGTAGTAGAGAGCGATCAAAATGGGAAAGCATATTAGATGAATTTGAAAACTCCCAGAGCAAAGATATTAAAGATATTCTTCGGATAAGCTTTGATGGGCTTGAAGAAAAAGTAAAGGATATTTTCCTTGACATTTCTTGTCTGTTCGGGAGAGAGAAAGTTAATTACACGAAGAAGATGTTAAGTGCATGTCATCTCAATCTAGATTTTGGAATTATAGTTCTCATGGATTATTCACTCATTACGATTGTAAACGACGAGGTGCAAATGCATGATTTAATACGACAGATGGGTCATAAAATAGTTAATGGTGAATCTTCTGAACCTGGAAAGAGGAGTAGGTTGTGGTTGGATTCAGTGAGTAACTCATACCCATAAAGTATATTTATTTACTTATTTGCAAGACTTTGTGATGAAAAATGTTAGTCAAAGTAAGTTGTCAATTACAATAATAATGTTAAATTGATGACTTCGCAGGGAACAGATGCAGTTAAAGCCATAAAGTTGGACTTGCCTAAACCCACAAGGCTAAACGTTGATCCAAAAGCATTTAGAAACATGAAAAATCTTCGATTGCTTATCGTTCGAAATGCAACATTTTGTAAGAAGGTTGAGTATCTGCCTATTAACTTAAAGTGGATTGAGTAG

mRNA sequence

ATGAAATCCAAGGGACAGTTAGTTTTACCAGTCTTCTACAAGGTGGATCCATCCGAAGTACGAAAACAAACTGGTAGATTTGGTGAAGCATTGGCCAAACATGAAGCTAAGTTTATGAGCACCAAGAAAATTCAAACATGGAAGGAAGCTTTGACTATTGCTGCTGATTTGTCTGGTTGGAATTTAGGAACTAGAAAGGAGGCTGATCTAATTGAAGATCTTGTTAAAGAAGTGTTGTCGGTATTAAATCGCACTAGCACACCATTATATGTAGCTAAGTATCCAGTTGGACTTGACTCTCAATTATTAGGACAGTTGAATTTTCACTCCCAATATGGGACACAACATTTGTCTACTGGTGTTTACATGTTGGGGATTTATGGCATTGGAGGTATTGGCAAGACAACTTTCGCCAAAGCTTTATTCAACAAAATTGCTCATCAATTTGAAGGTTCTTGTTTTCTATTTAATGTTAGAGAAACTTCAAAGCAGTTCAATGGCCTAATTCAACTACAAGAAAAGCTACTCTATGAAATCTTAAAGGATGATTCGAAGATTGTCAATCTCGACGAAGGAATTAGTATCATAAGTAATAGACTGTGTTCAAAGAAAGTTCTTATAGTTCTTGATGATGTGGATAAGCTTGGACAATTACAAGCATTGGTTGGTGGTCGTGATTGGTTTGGTCGAGATAGTAAAATCATTGTGACAACAAGCCATGGATTTGATCAAATGCATGATATTCAAGGATTGAATCCTAAAAACGCTTTGGAGTTGTTTAGTTGGCATGCTTTTAAGAAAAGTCAACCATTAAGTAATTATTTAGACCTTTCAAAATGTGCTACGACTTATTGTAAAGGTCTTCCTTTGGCTCTTGTTGTTTTGGGTTCTTTCCTTTGTAGTAGAGAGCGATCAAAATGGGAAAGCATATTAGATGAATTTGAAAACTCCCAGAGCAAAGATATTAAAGATATTCTTCGGATAAGCTTTGATGGGCTTGAAGAAAAAGTAAAGGATATTTTCCTTGACATTTCTTGTCTGTTCGGGAGAGAGAAAGTTAATTACACGAAGAAGATGTTAAGTGCATGTCATCTCAATCTAGATTTTGGAATTATAGTTCTCATGGATTATTCACTCATTACGATTGTAAACGACGAGGTGCAAATGCATGATTTAATACGACAGATGGGTCATAAAATAGTTAATGGTGAATCTTCTGAACCTGGAAAGAGGAGTAGGTTGTGGTTGGATTCAGGAACAGATGCAGTTAAAGCCATAAAGTTGGACTTGCCTAAACCCACAAGGCTAAACGTTGATCCAAAAGCATTTAGAAACATGAAAAATCTTCGATTGCTTATCGTTCGAAATGCAACATTTTGTAAGAAGGTTGAGTATCTGCCTATTAACTTAAAGTGGATTGAGTAG

Coding sequence (CDS)

ATGAAATCCAAGGGACAGTTAGTTTTACCAGTCTTCTACAAGGTGGATCCATCCGAAGTACGAAAACAAACTGGTAGATTTGGTGAAGCATTGGCCAAACATGAAGCTAAGTTTATGAGCACCAAGAAAATTCAAACATGGAAGGAAGCTTTGACTATTGCTGCTGATTTGTCTGGTTGGAATTTAGGAACTAGAAAGGAGGCTGATCTAATTGAAGATCTTGTTAAAGAAGTGTTGTCGGTATTAAATCGCACTAGCACACCATTATATGTAGCTAAGTATCCAGTTGGACTTGACTCTCAATTATTAGGACAGTTGAATTTTCACTCCCAATATGGGACACAACATTTGTCTACTGGTGTTTACATGTTGGGGATTTATGGCATTGGAGGTATTGGCAAGACAACTTTCGCCAAAGCTTTATTCAACAAAATTGCTCATCAATTTGAAGGTTCTTGTTTTCTATTTAATGTTAGAGAAACTTCAAAGCAGTTCAATGGCCTAATTCAACTACAAGAAAAGCTACTCTATGAAATCTTAAAGGATGATTCGAAGATTGTCAATCTCGACGAAGGAATTAGTATCATAAGTAATAGACTGTGTTCAAAGAAAGTTCTTATAGTTCTTGATGATGTGGATAAGCTTGGACAATTACAAGCATTGGTTGGTGGTCGTGATTGGTTTGGTCGAGATAGTAAAATCATTGTGACAACAAGCCATGGATTTGATCAAATGCATGATATTCAAGGATTGAATCCTAAAAACGCTTTGGAGTTGTTTAGTTGGCATGCTTTTAAGAAAAGTCAACCATTAAGTAATTATTTAGACCTTTCAAAATGTGCTACGACTTATTGTAAAGGTCTTCCTTTGGCTCTTGTTGTTTTGGGTTCTTTCCTTTGTAGTAGAGAGCGATCAAAATGGGAAAGCATATTAGATGAATTTGAAAACTCCCAGAGCAAAGATATTAAAGATATTCTTCGGATAAGCTTTGATGGGCTTGAAGAAAAAGTAAAGGATATTTTCCTTGACATTTCTTGTCTGTTCGGGAGAGAGAAAGTTAATTACACGAAGAAGATGTTAAGTGCATGTCATCTCAATCTAGATTTTGGAATTATAGTTCTCATGGATTATTCACTCATTACGATTGTAAACGACGAGGTGCAAATGCATGATTTAATACGACAGATGGGTCATAAAATAGTTAATGGTGAATCTTCTGAACCTGGAAAGAGGAGTAGGTTGTGGTTGGATTCAGGAACAGATGCAGTTAAAGCCATAAAGTTGGACTTGCCTAAACCCACAAGGCTAAACGTTGATCCAAAAGCATTTAGAAACATGAAAAATCTTCGATTGCTTATCGTTCGAAATGCAACATTTTGTAAGAAGGTTGAGTATCTGCCTATTAACTTAAAGTGGATTGAGTAG

Protein sequence

MKSKGQLVLPVFYKVDPSEVRKQTGRFGEALAKHEAKFMSTKKIQTWKEALTIAADLSGWNLGTRKEADLIEDLVKEVLSVLNRTSTPLYVAKYPVGLDSQLLGQLNFHSQYGTQHLSTGVYMLGIYGIGGIGKTTFAKALFNKIAHQFEGSCFLFNVRETSKQFNGLIQLQEKLLYEILKDDSKIVNLDEGISIISNRLCSKKVLIVLDDVDKLGQLQALVGGRDWFGRDSKIIVTTSHGFDQMHDIQGLNPKNALELFSWHAFKKSQPLSNYLDLSKCATTYCKGLPLALVVLGSFLCSRERSKWESILDEFENSQSKDIKDILRISFDGLEEKVKDIFLDISCLFGREKVNYTKKMLSACHLNLDFGIIVLMDYSLITIVNDEVQMHDLIRQMGHKIVNGESSEPGKRSRLWLDSGTDAVKAIKLDLPKPTRLNVDPKAFRNMKNLRLLIVRNATFCKKVEYLPINLKWIE
Homology
BLAST of Lag0006652 vs. NCBI nr
Match: XP_038877735.1 (disease resistance protein RPS6-like, partial [Benincasa hispida])

HSP 1 Score: 701.8 bits (1810), Expect = 4.0e-198
Identity = 364/489 (74.44%), Postives = 410/489 (83.84%), Query Frame = 0

Query: 2   KSKGQLVLPVFYKVDPSEVRKQTGRFGEALAKHEAKFMSTKKIQTWKEALTIAADLSGWN 61
           KSKGQ+VLP+FYKV+PS++RKQTG FGEALAKH+AKFM  +K Q W+EALT AA+LSGW+
Sbjct: 33  KSKGQIVLPIFYKVNPSDIRKQTGSFGEALAKHQAKFM--EKTQIWREALTTAANLSGWD 92

Query: 62  LGTRKEADLIEDLVKEVLSVLNRTSTPLYVAKYPVGLDSQLLGQLNFHSQYGTQHLSTGV 121
           LGTRKEAD I+DLVKEVLS LN  +  LYVAKYPVG+DS+ L  +  HS +  QH+S GV
Sbjct: 93  LGTRKEADFIQDLVKEVLSRLNCANRQLYVAKYPVGIDSK-LEDMKLHS-HRIQHVSDGV 152

Query: 122 YMLGIYGIGGIGKTTFAKALFNKIAHQFEGSCFLFNVRETSKQFNGLIQLQEKLLYEILK 181
           YM+GIYGIGGIGKTT AKAL+NKIAHQFEG CFL NVRE SKQFNGL+Q QEKLLYEILK
Sbjct: 153 YMMGIYGIGGIGKTTLAKALYNKIAHQFEGFCFLSNVREASKQFNGLVQQQEKLLYEILK 212

Query: 182 DDSKIVNLDEGISIISNRLCSKKVLIVLDDVDKLGQLQALVGGRDWFGRDSKIIVTT--- 241
           DD K+ NLDEGI+II +RL SKKVL+VLDDVDKL QL+ALVGGRDWFG  SKIIVTT   
Sbjct: 213 DDLKVGNLDEGINIIRSRLRSKKVLVVLDDVDKLEQLEALVGGRDWFGPGSKIIVTTRNS 272

Query: 242 ----SHGFDQMHDIQGLNPKNALELFSWHAFKKSQPLSNYLDLSKCATTYCKGLPLALVV 301
               SH FD+ + IQGL+   ALELFSWHAFKKS P SNYLDLS+ AT+YCKG PLALVV
Sbjct: 273 HLLSSHEFDETYGIQGLSRDYALELFSWHAFKKSHPSSNYLDLSERATSYCKGHPLALVV 332

Query: 302 LGSFLCSRERSKWESILDEFENSQSKDIKDILRISFDGLEEKVKDIFLDISCLFGREKVN 361
           LGSFLCSR+++KW++ILDEFENS SKDIKDIL+ISFDGLEEKVK+IFLDISCL   EKVN
Sbjct: 333 LGSFLCSRDQTKWKTILDEFENSLSKDIKDILQISFDGLEEKVKEIFLDISCLLVGEKVN 392

Query: 362 YTKKMLSACHLNLDFGIIVLMDYSLITIVNDEVQMHDLIRQMGHKIVNGESSEPGKRSRL 421
           Y K +L+ CH NLDFGI+VLMD SLIT  NDEVQMHDLIRQMG KIVNGESSEPG+RSRL
Sbjct: 393 YVKHILNTCHFNLDFGIVVLMDLSLITFENDEVQMHDLIRQMGRKIVNGESSEPGERSRL 452

Query: 422 WL----------DSGTDAVKAIKLDLPKPTRLNVDPKAFRNMKNLRLLIVRNATFCKKVE 474
           WL          +SGT AVKAIKLDLP PTRL+VDP+AF NM+NLRLLIVRNATF   +E
Sbjct: 453 WLVHDVLKVFIDNSGTSAVKAIKLDLPNPTRLDVDPRAFSNMQNLRLLIVRNATFSTNLE 512

BLAST of Lag0006652 vs. NCBI nr
Match: TYK18296.1 (disease resistance protein RPP4-like [Cucumis melo var. makuwa])

HSP 1 Score: 686.8 bits (1771), Expect = 1.3e-193
Identity = 358/502 (71.31%), Postives = 408/502 (81.27%), Query Frame = 0

Query: 2   KSKGQLVLPVFYKVDPSEVRKQTGRFGEALAKHEAKFMSTKKIQTWKEALTIAADLSGWN 61
           KSKGQ+VLPVFYKVDPS++RKQ+G FGEALAKH+AKF +  KIQ W+EALTIA +LSGW+
Sbjct: 99  KSKGQIVLPVFYKVDPSDIRKQSGSFGEALAKHQAKFQT--KIQIWREALTIAGNLSGWD 158

Query: 62  LGTRKEADLIEDLVKEVLSVLNRTSTPLYVAKYPVGLDSQL-LGQLNFHSQYG------- 121
           LGTRKEADLI DLVK+VLS LNRT TPLYVAKYPVG+DS L   +L  H+ +G       
Sbjct: 159 LGTRKEADLIGDLVKKVLSTLNRTCTPLYVAKYPVGIDSNLEYMKLRSHNLFGKSNKLHY 218

Query: 122 -TQH---LSTGVYMLGIYGIGGIGKTTFAKALFNKIAHQFEGSCFLFNVRETSKQFNGLI 181
            TQH     T VYM+GIYGIGGIGKTT AKAL+NKIA QFEG CFL NVRE SKQFNGL 
Sbjct: 219 ETQHEYEFDTSVYMVGIYGIGGIGKTTLAKALYNKIASQFEGCCFLSNVREASKQFNGLA 278

Query: 182 QLQEKLLYEILKDDSKIVNLDEGISIISNRLCSKKVLIVLDDVDKLGQLQALVGGRDWFG 241
           QLQE LLYEIL    K++NLD GI+II NRLCSKKVLIVLDDVD+L QL+ALVGGRDWFG
Sbjct: 279 QLQESLLYEILMVHLKVINLDRGINIIRNRLCSKKVLIVLDDVDRLEQLEALVGGRDWFG 338

Query: 242 RDSKIIVTT-------SHGFDQMHDIQGLNPKNALELFSWHAFKKSQPLSNYLDLSKCAT 301
           + S+IIVTT       +HGFD++H+I GLN   A+ELFSWHAFKK+ P SNYLDLSK AT
Sbjct: 339 KGSRIIVTTRNKHLLFTHGFDEIHNILGLNEDKAIELFSWHAFKKNSPSSNYLDLSKRAT 398

Query: 302 TYCKGLPLALVVLGSFLCSRERSKWESILDEFENSQSKDIKDILRISFDGLEEKVKDIFL 361
            YC+G PLAL+VLGSFLC+R++ +W SILDEFENS +KDIKDIL++SFDGLEEK+KDIFL
Sbjct: 399 IYCEGHPLALIVLGSFLCTRDQVEWCSILDEFENSLNKDIKDILQLSFDGLEEKIKDIFL 458

Query: 362 DISCLFGREKVNYTKKMLSACHLNLDFGIIVLMDYSLITIVNDEVQMHDLIRQMGHKIVN 421
           DISCL   EKV Y K MLSACH NLDFGIIVLMD SL+TI ND+VQMHDLI+QMGHKIV 
Sbjct: 459 DISCLLVGEKVKYVKNMLSACHANLDFGIIVLMDLSLMTIENDKVQMHDLIKQMGHKIVC 518

Query: 422 GESSEPGKRSRLWL----------DSGTDAVKAIKLDLPKPTRLNVDPKAFRNMKNLRLL 475
           GES E GKRSRLWL          +SG DAVKAIKLD P PTRL+VD +AFR MKNLRLL
Sbjct: 519 GESLELGKRSRLWLVQDVWDVLVNNSGIDAVKAIKLDFPNPTRLDVDLQAFRKMKNLRLL 578

BLAST of Lag0006652 vs. NCBI nr
Match: XP_016901470.1 (PREDICTED: LOW QUALITY PROTEIN: disease resistance protein RPP4-like [Cucumis melo])

HSP 1 Score: 686.4 bits (1770), Expect = 1.7e-193
Identity = 357/502 (71.12%), Postives = 409/502 (81.47%), Query Frame = 0

Query: 2   KSKGQLVLPVFYKVDPSEVRKQTGRFGEALAKHEAKFMSTKKIQTWKEALTIAADLSGWN 61
           KSKGQ+VLP+FYKVDPS++RKQ+G FGEALAKH+AKF +  K Q W+EALT AA+LSGW+
Sbjct: 121 KSKGQIVLPIFYKVDPSDIRKQSGTFGEALAKHQAKFQT--KTQIWREALTTAANLSGWD 180

Query: 62  LGTRKEADLIEDLVKEVLSVLNRTSTPLYVAKYPVGLDSQL-LGQLNFHS--------QY 121
           LGTRKEADLI DLVK VLS LNRT TPLYVAKYPVG+DS+L   +L  H+         Y
Sbjct: 181 LGTRKEADLIGDLVKNVLSTLNRTCTPLYVAKYPVGIDSKLEYMKLQSHNLFEKSNKFHY 240

Query: 122 GTQH---LSTGVYMLGIYGIGGIGKTTFAKALFNKIAHQFEGSCFLFNVRETSKQFNGLI 181
            TQH     TGVY++GIYGIGGIGKTT AKAL+NKIA QFEG CFL NVRE SKQFNGL 
Sbjct: 241 QTQHEYEFDTGVYLVGIYGIGGIGKTTLAKALYNKIASQFEGCCFLSNVREASKQFNGLA 300

Query: 182 QLQEKLLYEILKDDSKIVNLDEGISIISNRLCSKKVLIVLDDVDKLGQLQALVGGRDWFG 241
           QLQE LLYEIL  D K+VN+D GI+II NRLCSKKVLIVLDDVDKL QL+ LVGGRDWFG
Sbjct: 301 QLQESLLYEILTVDLKVVNIDRGINIIRNRLCSKKVLIVLDDVDKLEQLETLVGGRDWFG 360

Query: 242 RDSKIIVTT-------SHGFDQMHDIQGLNPKNALELFSWHAFKKSQPLSNYLDLSKCAT 301
           + S+I+VTT       SHGFD++H+I GLN   A+ELFSWHAFKK+ P SNY DLSK AT
Sbjct: 361 QGSRIMVTTRNKHLLSSHGFDEIHNILGLNEDKAIELFSWHAFKKNSPSSNYXDLSKRAT 420

Query: 302 TYCKGLPLALVVLGSFLCSRERSKWESILDEFENSQSKDIKDILRISFDGLEEKVKDIFL 361
           +YCKG PLALVVLGSFLC+R++ +W SILDEFENS +KDIKDIL++SFDGLE+K+KDIFL
Sbjct: 421 SYCKGHPLALVVLGSFLCTRDQVEWCSILDEFENSLNKDIKDILQLSFDGLEDKIKDIFL 480

Query: 362 DISCLFGREKVNYTKKMLSACHLNLDFGIIVLMDYSLITIVNDEVQMHDLIRQMGHKIVN 421
           DISCL   EKV Y K MLSACH+NLDFGIIVLMD SL+TI ND+VQM+DLI+QMGHKIV 
Sbjct: 481 DISCLLVGEKVKYVKNMLSACHVNLDFGIIVLMDLSLMTIENDKVQMYDLIKQMGHKIVC 540

Query: 422 GESSEPGKRSRLWL----------DSGTDAVKAIKLDLPKPTRLNVDPKAFRNMKNLRLL 475
           GES E GKRSRLWL          +SGTDAVKAIKLD P PT+L+VD +AFR MKNLRLL
Sbjct: 541 GESLELGKRSRLWLVRDVWEVLVNNSGTDAVKAIKLDFPNPTKLDVDLQAFRKMKNLRLL 600

BLAST of Lag0006652 vs. NCBI nr
Match: KAA0047973.1 (TIR-NBS-LRR disease resistance protein [Cucumis melo var. makuwa])

HSP 1 Score: 683.3 bits (1762), Expect = 1.5e-192
Identity = 357/502 (71.12%), Postives = 407/502 (81.08%), Query Frame = 0

Query: 2   KSKGQLVLPVFYKVDPSEVRKQTGRFGEALAKHEAKFMSTKKIQTWKEALTIAADLSGWN 61
           KSK Q+VLPVFYKVDPS++RKQ+G FGEALAKH+AKF +  KIQ W+EALT AA+LSGW+
Sbjct: 99  KSKDQIVLPVFYKVDPSDIRKQSGSFGEALAKHQAKFKT--KIQIWREALTTAANLSGWD 158

Query: 62  LGTRKEADLIEDLVKEVLSVLNRTSTPLYVAKYPVGLDSQL---------LGQLNFHSQY 121
           LGTRKEADLI D+VK+VLS LNRT  PLYVAKYPVG+DS+L         L + N    Y
Sbjct: 159 LGTRKEADLIGDIVKKVLSTLNRTCMPLYVAKYPVGIDSKLEYIKLRSHNLFEKNNKFHY 218

Query: 122 GTQH---LSTGVYMLGIYGIGGIGKTTFAKALFNKIAHQFEGSCFLFNVRETSKQFNGLI 181
            TQH     TG+YM+GIYGIGGIGKTT AKAL+NKIA QFEG CFL NVRE SKQFNGL 
Sbjct: 219 RTQHEYEFDTGIYMVGIYGIGGIGKTTLAKALYNKIASQFEGCCFLSNVREASKQFNGLA 278

Query: 182 QLQEKLLYEILKDDSKIVNLDEGISIISNRLCSKKVLIVLDDVDKLGQLQALVGGRDWFG 241
           QLQE LLYEIL  D K+VNLD GI+II NRLCSKKVLIVLDDVDKL QL+ALVGG DWFG
Sbjct: 279 QLQESLLYEILMVDLKVVNLDRGINIIRNRLCSKKVLIVLDDVDKLEQLEALVGGCDWFG 338

Query: 242 RDSKIIVTT-------SHGFDQMHDIQGLNPKNALELFSWHAFKKSQPLSNYLDLSKCAT 301
           + S+IIVTT       SHGFD++H+I GLN   A+ELFSWHAFKK++P SNYLDLSK AT
Sbjct: 339 KGSRIIVTTRNKHLLFSHGFDEIHNILGLNEDKAIELFSWHAFKKNRPSSNYLDLSKRAT 398

Query: 302 TYCKGLPLALVVLGSFLCSRERSKWESILDEFENSQSKDIKDILRISFDGLEEKVKDIFL 361
           +YCKG PLALVVLGSFLC R++++W SILDEFENS +KDIKDIL++SFDGLE+K+KDIFL
Sbjct: 399 SYCKGHPLALVVLGSFLCIRDQAEWCSILDEFENSLNKDIKDILQLSFDGLEDKIKDIFL 458

Query: 362 DISCLFGREKVNYTKKMLSACHLNLDFGIIVLMDYSLITIVNDEVQMHDLIRQMGHKIVN 421
           DISCL   EKV Y K MLSACH+NLDFG+IVLMD SLITI ND+VQMHDLI+QMG KIV 
Sbjct: 459 DISCLLVGEKVEYVKDMLSACHVNLDFGVIVLMDLSLITIENDKVQMHDLIKQMGQKIVC 518

Query: 422 GESSEPGKRSRLWL----------DSGTDAVKAIKLDLPKPTRLNVDPKAFRNMKNLRLL 475
           GES E GKRSRLWL          +SGTDA+KAIKLD P PTRL V+ +AFR MKNLRLL
Sbjct: 519 GESLELGKRSRLWLVHDVWEVLVNNSGTDAIKAIKLDFPNPTRLGVNSQAFRKMKNLRLL 578

BLAST of Lag0006652 vs. NCBI nr
Match: ADN33675.1 (TIR-NBS-LRR disease resistance protein [Cucumis melo subsp. melo])

HSP 1 Score: 681.0 bits (1756), Expect = 7.3e-192
Identity = 355/502 (70.72%), Postives = 406/502 (80.88%), Query Frame = 0

Query: 2   KSKGQLVLPVFYKVDPSEVRKQTGRFGEALAKHEAKFMSTKKIQTWKEALTIAADLSGWN 61
           KSK Q+VLPVFYKVDPS++RKQ+G FGEALAKH+AKF +  KIQ W+EALT AA+LSGW+
Sbjct: 99  KSKDQIVLPVFYKVDPSDIRKQSGSFGEALAKHQAKFKT--KIQIWREALTTAANLSGWD 158

Query: 62  LGTRKEADLIEDLVKEVLSVLNRTSTPLYVAKYPVGLDSQL---------LGQLNFHSQY 121
           LGTRKEADLI D+VK+VLS LNRT  PLYVAKYPVG+DS+L         + + N    Y
Sbjct: 159 LGTRKEADLIGDIVKKVLSTLNRTCMPLYVAKYPVGIDSKLEYIKLRSHNMFEKNNKFHY 218

Query: 122 GTQH---LSTGVYMLGIYGIGGIGKTTFAKALFNKIAHQFEGSCFLFNVRETSKQFNGLI 181
            TQH     TG+YM+GIYGIGGIGKTT AKAL+NKIA QFEG CFL NVRE SKQFNGL 
Sbjct: 219 RTQHEYEFDTGIYMVGIYGIGGIGKTTLAKALYNKIASQFEGCCFLSNVREASKQFNGLA 278

Query: 182 QLQEKLLYEILKDDSKIVNLDEGISIISNRLCSKKVLIVLDDVDKLGQLQALVGGRDWFG 241
           QLQE LLYEIL  D K+VNLD GI+II NRLCSKKVLIVLDDVDKL QL+ALVGG DWFG
Sbjct: 279 QLQESLLYEILMVDLKVVNLDRGINIIRNRLCSKKVLIVLDDVDKLEQLEALVGGCDWFG 338

Query: 242 RDSKIIVTT-------SHGFDQMHDIQGLNPKNALELFSWHAFKKSQPLSNYLDLSKCAT 301
           + S+IIVTT       SHGFD++H+I GLN   A+ELFSWHAFKK++P SNYLDLSK AT
Sbjct: 339 KGSRIIVTTRNKHLLFSHGFDEIHNILGLNEDKAIELFSWHAFKKNRPSSNYLDLSKRAT 398

Query: 302 TYCKGLPLALVVLGSFLCSRERSKWESILDEFENSQSKDIKDILRISFDGLEEKVKDIFL 361
           +YCKG PLALVVLGSFLC R++++W SILDEFENS +KDIKDIL++SFDGLE+K+KDIFL
Sbjct: 399 SYCKGHPLALVVLGSFLCIRDQAEWCSILDEFENSLNKDIKDILQLSFDGLEDKIKDIFL 458

Query: 362 DISCLFGREKVNYTKKMLSACHLNLDFGIIVLMDYSLITIVNDEVQMHDLIRQMGHKIVN 421
           DISCL   EKV Y K ML ACH+NLDFG+IVLMD SLITI ND+VQMHDLI+QMG KIV 
Sbjct: 459 DISCLLVGEKVEYVKDMLGACHVNLDFGVIVLMDLSLITIENDKVQMHDLIKQMGQKIVC 518

Query: 422 GESSEPGKRSRLWL----------DSGTDAVKAIKLDLPKPTRLNVDPKAFRNMKNLRLL 475
           GES E GKRSRLWL          +SGTDA+KAIKLD P PTRL V+ +AFR MKNLRLL
Sbjct: 519 GESLELGKRSRLWLVQDVWEVLVNNSGTDAIKAIKLDFPNPTRLGVNSQAFRKMKNLRLL 578

BLAST of Lag0006652 vs. ExPASy Swiss-Prot
Match: V9M2S5 (Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=1)

HSP 1 Score: 306.6 bits (784), Expect = 4.9e-82
Identity = 192/479 (40.08%), Postives = 278/479 (58.04%), Query Frame = 0

Query: 2   KSKGQLVLPVFYKVDPSEVRKQTGRFGEALAKHEAKFMSTKKIQTWKEALTIAADLSGWN 61
           K  G  V P+FY VDPS VRKQ G FGEA A +E  +    KI  W+ ALT AA+LSGW+
Sbjct: 106 KDLGHAVFPIFYHVDPSHVRKQEGSFGEAFAGYEENWKD--KIPRWRTALTEAANLSGWH 165

Query: 62  -LGTRKEADLIEDLVKEVLSVLNRTSTPLYVAKYPVGLDSQLLGQ-LNFHSQYGTQHLST 121
            L  R E++ I+++   +   L      L V    VG+DS +    L  H +      S+
Sbjct: 166 LLDDRYESNQIKEITNSIFRQLK--CKRLDVGANLVGIDSHVKEMILRLHLE------SS 225

Query: 122 GVYMLGIYGIGGIGKTTFAKALFNKIAHQFEGSCFLFNVRETSKQFNGLIQLQEKLLYEI 181
            V M+GIYG+GGIGKTT AK ++N+++ +FE   FL N+RE S     L  LQ +LL +I
Sbjct: 226 DVRMVGIYGVGGIGKTTIAKVIYNELSCEFEYMSFLENIREGSNP-QVLFHLQNQLLGDI 285

Query: 182 LKDD--SKIVNLDEGISIISNRLCSKKVLIVLDDVDKLGQLQALVGGRDWFGRDSKIIVT 241
           L+ +    I ++    S+I + L S++V IVLDDVD L QL+ L+G R+W G  S++I+T
Sbjct: 286 LEGEGSQNISSVAHRASMIKDILLSRRVFIVLDDVDDLSQLEYLLGHREWLGEGSRVIIT 345

Query: 242 TSH-------GFDQMHDIQGLNPKNALELFSWHAFKKSQPLSNYLDLSKCATTYCKGLPL 301
           T +         D +++++GLN + A ELFS +AFK++ P S+Y +L+     YC+GLPL
Sbjct: 346 TRNKHVLAVQEVDDLYEVEGLNFEEACELFSLYAFKQNLPKSDYRNLTCRVVGYCQGLPL 405

Query: 302 ALVVLGSFLCSRERSKWESILDEFENSQSKDIKDILRISFDGLEEKVKDIFLDISCLFGR 361
           AL VLGS LC +   +WE  L + ++    DI  +L+ S+DGL+   K+IFLD++C F  
Sbjct: 406 ALKVLGSLLCKKTIPQWEGELKKLDSEPKADIHKVLKRSYDGLDRIDKNIFLDLACFFKG 465

Query: 362 EKVNYTKKMLSACHLNLDFGIIVLMDYSLITIVNDEVQMHDLIRQMGHKIVNGE-SSEPG 421
           E  ++  ++L  C    + GI  L D  LIT+  +++ MHDLI+QMG +IV      EP 
Sbjct: 466 EGRDFVLRILDGCDFPAETGISNLNDLCLITLPYNQICMHDLIQQMGWEIVRENFPVEPN 525

Query: 422 KRSRLW----------LDSGTDAVKAIKLDLPKPTRLNVDPKAFRNMKNLRLLIVRNAT 459
           K SRLW           D G  +V+ + LDL K  R+  +   F  M  LRLL V +++
Sbjct: 526 KWSRLWDPCDFERALTADEGIKSVETMSLDLSKLKRVCSNSNVFAKMTKLRLLKVYSSS 573

BLAST of Lag0006652 vs. ExPASy Swiss-Prot
Match: V9M398 (Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=1)

HSP 1 Score: 298.1 bits (762), Expect = 1.7e-79
Identity = 190/479 (39.67%), Postives = 272/479 (56.78%), Query Frame = 0

Query: 2   KSKGQLVLPVFYKVDPSEVRKQTGRFGEALAKHEAKFMSTKKIQTWKEALTIAADLSGWN 61
           K  G  V P+FY VDPS VRKQ G FGEA A +        KI  W+ ALT AA+LSGW 
Sbjct: 112 KDPGHAVFPIFYHVDPSHVRKQEGSFGEAFAGYGENLKD--KIPRWRTALTEAANLSGWP 171

Query: 62  LGTRKEADLIEDLVKEVLSVLNRTSTPLYVAKYPVGLDSQLLGQL-NFHSQYGTQHLSTG 121
           L    E++ I+++   +   L      L      VG+DS +   +   H +      S+ 
Sbjct: 172 LQDGYESNQIKEITDSIFRRLK--CKRLDAGANLVGIDSHVKEMIWRLHME------SSD 231

Query: 122 VYMLGIYGIGGIGKTTFAKALFNKIAHQFEGSCFLFNVRETSKQFN--GLIQLQEKLLYE 181
           V M+G+YG+GGIGKTT AK ++N+++ +FE   FL N+RE   +FN  G+  LQ +LL +
Sbjct: 232 VRMVGMYGVGGIGKTTIAKVIYNELSREFEYMSFLENIRE---KFNTQGVSPLQNQLLDD 291

Query: 182 ILKDD--SKIVNLDEGISIISNRLCSKKVLIVLDDVDKLGQLQALVGGRDWFGRDSKIIV 241
           ILK +    I ++  G S+I + L SK V IVLDDVD   QL+ L+  R+W G  S++I+
Sbjct: 292 ILKGEGSQNINSVAHGASMIKDILSSKIVFIVLDDVDDQSQLEYLLRHREWLGEGSRVII 351

Query: 242 TTSH-------GFDQMHDIQGLNPKNALELFSWHAFKKSQPLSNYLDLSKCATTYCKGLP 301
           TT +         D +++++GLN + A ELFS +AF+++ P S+Y +LS     YC+GLP
Sbjct: 352 TTRNKHVLDVQKVDDLYEVKGLNFEEACELFSLYAFEQNLPKSDYRNLSHRVVGYCQGLP 411

Query: 302 LALVVLGSFLCSRERSKWESILDEFENSQSKDIKDILRISFDGLEEKVKDIFLDISCLFG 361
           LAL VLG  L  +   +WES L + +     +I  +L+ S+DGL    K IFLD++C F 
Sbjct: 412 LALKVLGCLLLKKTIPEWESELRKLDREPEAEILSVLKRSYDGLGRTEKSIFLDVACFFK 471

Query: 362 REKVNYTKKMLSACHLNLDFGIIVLMDYSLITIVNDEVQMHDLIRQMGHKIVNGE-SSEP 421
            E  ++  K+L AC  + + GI  L D  LIT+  + ++MHDLI+QMG +IV  +   EP
Sbjct: 472 GEDRDFVSKILDACDFHAEIGIKNLNDKCLITLQYNRIRMHDLIQQMGWEIVREKFPDEP 531

Query: 422 GKRSRLW----------LDSGTDAVKAIKLDLPKPTRLNVDPKAFRNMKNLRLLIVRNA 458
            K SRLW             G   V+ I LDL K  R+  +  AF  M  LRLL V+++
Sbjct: 532 NKWSRLWDTCDFERALTAYKGIKRVETISLDLSKLKRVCSNSNAFAKMTRLRLLKVQSS 577

BLAST of Lag0006652 vs. ExPASy Swiss-Prot
Match: Q40392 (TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 4.3e-78
Identity = 186/491 (37.88%), Postives = 292/491 (59.47%), Query Frame = 0

Query: 6   QLVLPVFYKVDPSEVRKQTGRFGEALAKHEAKFM-STKKIQTWKEALTIAADLSG-WNLG 65
           Q V+P+FY VDPS VR Q   F +A  +HE K+    + IQ W+ AL  AA+L G  +  
Sbjct: 99  QTVIPIFYDVDPSHVRNQKESFAKAFEEHETKYKDDVEGIQRWRIALNEAANLKGSCDNR 158

Query: 66  TRKEADLIEDLVKEVLSVLNRTSTPLYVAKYPVGLDSQLLGQLNFHSQYGTQHLSTGVYM 125
            + +AD I  +V ++ S L + S  L   +  VG+D+  L ++    + G      GV +
Sbjct: 159 DKTDADCIRQIVDQISSKLCKIS--LSYLQNIVGIDTH-LEKIESLLEIGI----NGVRI 218

Query: 126 LGIYGIGGIGKTTFAKALFNKI------AHQFEGSCFLFNVRETSKQFNGLIQLQEKLLY 185
           +GI+G+GG+GKTT A+A+F+ +      ++QF+G+CFL +++E  +   G+  LQ  LL 
Sbjct: 219 MGIWGMGGVGKTTIARAIFDTLLGRMDSSYQFDGACFLKDIKENKR---GMHSLQNALLS 278

Query: 186 EILKDDSKIVNLDEGISIISNRLCSKKVLIVLDDVD-KLGQLQALVGGRDWFGRDSKIIV 245
           E+L++ +   N ++G   +++RL SKKVLIVLDD+D K   L+ L G  DWFG  S+II+
Sbjct: 279 ELLREKANYNNEEDGKHQMASRLRSKKVLIVLDDIDNKDHYLEYLAGDLDWFGNGSRIII 338

Query: 246 TTSHGF-----DQMHDIQGLNPKNALELFSWHAFKKSQPLSNYLDLSKCATTYCKGLPLA 305
           TT         D ++++  L    +++LF  HAF K  P  N+  LS     Y KGLPLA
Sbjct: 339 TTRDKHLIEKNDIIYEVTALPDHESIQLFKQHAFGKEVPNENFEKLSLEVVNYAKGLPLA 398

Query: 306 LVVLGSFLCSRERSKWESILDEFENSQSKDIKDILRISFDGLEEKVKDIFLDISCLFGRE 365
           L V GS L +   ++W+S ++  +N+    I D L+IS+DGLE K +++FLDI+C    E
Sbjct: 399 LKVWGSLLHNLRLTEWKSAIEHMKNNSYSGIIDKLKISYDGLEPKQQEMFLDIACFLRGE 458

Query: 366 KVNYTKKMLSACHLNLDFGIIVLMDYSLITIVN-DEVQMHDLIRQMGHKIVNGESSEPGK 425
           + +Y  ++L +CH+  ++G+ +L+D SL+ I   ++VQMHDLI+ MG  IVN +  +PG+
Sbjct: 459 EKDYILQILESCHIGAEYGLRILIDKSLVFISEYNQVQMHDLIQDMGKYIVNFQ-KDPGE 518

Query: 426 RSRLWL----------DSGTDAVKAIKLDLPKPTRLNVDPKAFRNMKNLRLLIVRNATFC 472
           RSRLWL          ++GT A++AI +     T L    +A +NMK LR+  +  ++  
Sbjct: 519 RSRLWLAKEVEEVMSNNTGTMAMEAIWVSSYSST-LRFSNQAVKNMKRLRVFNMGRSSTH 577

BLAST of Lag0006652 vs. ExPASy Swiss-Prot
Match: A0A290U7C4 (Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 5.6e-70
Identity = 179/501 (35.73%), Postives = 274/501 (54.69%), Query Frame = 0

Query: 7   LVLPVFYKVDPSEVRKQTGRFGEALAKHEAKFMSTK-KIQTWKEALTIAADLSGWNLGTR 66
           +V+PVFY VDPS VRKQ G +     K EA  +  + K+  W+EALT  A++SG +L   
Sbjct: 100 IVVPVFYDVDPSTVRKQNGEYAVCFTKFEANLVDDRDKVLRWREALTKVANISGHDLRNT 159

Query: 67  ---KEADLIEDLVKEV-------LSVLNRTSTPLYVAKYPVGLDSQLLGQLNFHSQYGTQ 126
               E+  I+ ++K++       +S+ NR           VG++SQ + +L+   +   +
Sbjct: 160 YNGDESKCIQQILKDIFDKFCFSISITNRDL---------VGIESQ-IKKLSSLLRMDLK 219

Query: 127 HLSTGVYMLGIYGIGGIGKTTFAKALFNKIAHQFEGSCFLFNVRETSKQFNGLIQLQEKL 186
               GV ++GI+G+GG+GKTT A+ALFN+    FE +CFL +V+E   Q + L+ LQ+ L
Sbjct: 220 ----GVRLVGIWGMGGVGKTTAARALFNRYYQNFESACFLEDVKE-YLQHHTLLYLQKTL 279

Query: 187 LYEILK-DDSKIVNLDEGISIISNRLCSKKVLIVLDDVDKLGQLQALVGGRDWFGRDSKI 246
           L ++LK +     + +E   I+  RLCSKKVL+VLDDV+   QL  LVG  DWFG  S+I
Sbjct: 280 LSKLLKVEFVDCTDTEEMCVILKRRLCSKKVLVVLDDVNHNDQLDKLVGAEDWFGSGSRI 339

Query: 247 IVTT-------SHGFDQMHDIQGLNPKNALELFSWHAFKKSQPLSNYLDLSKCATTYCKG 306
           ++TT       +H   + ++I+ L    A+ELF+ HAFK+S P   + +L      Y  G
Sbjct: 340 VITTRDMKLLKNHDVHETYEIKVLEKDEAIELFNLHAFKRSSPEKEFKELLNLVVDYTGG 399

Query: 307 LPLALVVLGSFLCSRERSKWESILDEFENSQSKDIKDILRISFDGLEEKVKDIFLDISCL 366
           LPLAL VLGS L   +   W S +D  +++   +I   L+ISFDGL +  K IFLDI+C 
Sbjct: 400 LPLALKVLGSLLYKEDLDVWISTIDRLKDNPEGEIMATLKISFDGLRDYEKSIFLDIACF 459

Query: 367 FGREKVNYTKKMLSACHLNLDFGIIVLMDYSLITIVNDEVQMHDLIRQMGHKIVNGES-- 426
           F          +  A   +   G+  L++ SLI I+ D++QMHDL+++MG +I   ES  
Sbjct: 460 FRGYNQRDMTALFHASGFHPVLGVKTLVEKSLIFILEDKIQMHDLMQEMGRQIAVQESPM 519

Query: 427 -----SEPGKRSRLWLDSGTDAVKAIKLDLPK-----PTRLNVDPKAFRNMKNLRLLIVR 475
                 E  K + +  D   +A++ + L  P+             +A +  + LR+L+  
Sbjct: 520 RRIYRPEDVKDACIG-DMRKEAIEGLLLTEPEQFEEGELEYMYSAEALKKTRRLRILVKE 579

BLAST of Lag0006652 vs. ExPASy Swiss-Prot
Match: Q40253 (Disease resistance protein L6 OS=Linum usitatissimum OX=4006 GN=L6 PE=1 SV=1)

HSP 1 Score: 265.4 bits (677), Expect = 1.2e-69
Identity = 176/494 (35.63%), Postives = 267/494 (54.05%), Query Frame = 0

Query: 6   QLVLPVFYKVDPSEVRKQTGRFGEALAKHEAKFMSTKKIQTWKEALTIAADLSGWNLGTR 65
           +++LP+FY VDPS+VR QTG + +A  KH  KF   + IQ WK+AL    DL GW++G  
Sbjct: 149 RIILPIFYMVDPSDVRHQTGCYKKAFRKHANKF-DGQTIQNWKDALKKVGDLKGWHIGKN 208

Query: 66  KEADLIEDLVK-EVLSVLNRTSTPLYVAKYPVGLDSQLLGQLNFHSQYGTQHLSTGVYML 125
            +   I D V  ++ S +++ +  L   +  VG+D  +   L   S       S  V M+
Sbjct: 209 DKQGAIADKVSADIWSHISKENLILETDEL-VGIDDHITAVLEKLSLD-----SENVTMV 268

Query: 126 GIYGIGGIGKTTFAKALFNKIAHQFEGSCFLFNVRETSKQFNGLIQLQEKLLYEILKDDS 185
           G+YG+GGIGKTT AKA++NKI+  F+  CF+ N+RET ++ +G++ LQ+KL+ EIL+ DS
Sbjct: 269 GLYGMGGIGKTTTAKAVYNKISSCFDCCCFIDNIRETQEK-DGVVVLQKKLVSEILRIDS 328

Query: 186 KIVNLDE---GISIISNRLCSKKVLIVLDDVDKLGQLQALVGGRDWFGRDSKIIVTT--- 245
             V  +    G   I  R+   K+L+VLDDVD+  + + ++G    F   S+ I+T+   
Sbjct: 329 GSVGFNNDSGGRKTIKERVSRFKILVVLDDVDEKFKFEDMLGSPKDFISQSRFIITSRSM 388

Query: 246 ------SHGFDQMHDIQGLNPKNALELFSWHAFKKSQPLSNYLDLSKCATTYCKGLPLAL 305
                 +    +++++  ++   +LELFS HAFKK+ P S Y  L+        GLPL L
Sbjct: 389 RVLGTLNENQCKLYEVGSMSKPRSLELFSKHAFKKNTPPSYYETLANDVVDTTAGLPLTL 448

Query: 306 VVLGSFLCSRERSKWESILDEFENSQSKD-IKDILRISFDGLEEKVKDIFLDISCLFGRE 365
            V+GS L  +E + WE  L++   + + D + D L+IS+D L  + K+IFLDI+C F  +
Sbjct: 449 KVIGSLLFKQEIAVWEDTLEQLRRTLNLDEVYDRLKISYDALNPEAKEIFLDIACFFIGQ 508

Query: 366 KVNYTKKMLSACHLNLDFGIIVLMDYSLITI-VNDEVQMHDLIRQMGHKIVNGESSEPGK 425
                  M + C+      II L+   +I +  +DE +MHD +R MG +IV  E   P K
Sbjct: 509 NKEEPYYMWTDCNFYPASNIIFLIQRCMIQVGDDDEFKMHDQLRDMGREIVRREDVLPWK 568

Query: 426 RSRLWL----------DSGTDAVKAIKLDLPKPTRLNVDPKAFRNMKNLRLLIVRNATFC 475
           RSR+W             G+  VKAI   +P   +     + F N+  LR L  R A   
Sbjct: 569 RSRIWSAEEGIDLLLNKKGSSKVKAI--SIPWGVKYEFKSECFLNLSELRYLHAREAMLT 628

BLAST of Lag0006652 vs. ExPASy TrEMBL
Match: A0A5D3D432 (Disease resistance protein RPP4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G00020 PE=4 SV=1)

HSP 1 Score: 686.8 bits (1771), Expect = 6.4e-194
Identity = 358/502 (71.31%), Postives = 408/502 (81.27%), Query Frame = 0

Query: 2   KSKGQLVLPVFYKVDPSEVRKQTGRFGEALAKHEAKFMSTKKIQTWKEALTIAADLSGWN 61
           KSKGQ+VLPVFYKVDPS++RKQ+G FGEALAKH+AKF +  KIQ W+EALTIA +LSGW+
Sbjct: 99  KSKGQIVLPVFYKVDPSDIRKQSGSFGEALAKHQAKFQT--KIQIWREALTIAGNLSGWD 158

Query: 62  LGTRKEADLIEDLVKEVLSVLNRTSTPLYVAKYPVGLDSQL-LGQLNFHSQYG------- 121
           LGTRKEADLI DLVK+VLS LNRT TPLYVAKYPVG+DS L   +L  H+ +G       
Sbjct: 159 LGTRKEADLIGDLVKKVLSTLNRTCTPLYVAKYPVGIDSNLEYMKLRSHNLFGKSNKLHY 218

Query: 122 -TQH---LSTGVYMLGIYGIGGIGKTTFAKALFNKIAHQFEGSCFLFNVRETSKQFNGLI 181
            TQH     T VYM+GIYGIGGIGKTT AKAL+NKIA QFEG CFL NVRE SKQFNGL 
Sbjct: 219 ETQHEYEFDTSVYMVGIYGIGGIGKTTLAKALYNKIASQFEGCCFLSNVREASKQFNGLA 278

Query: 182 QLQEKLLYEILKDDSKIVNLDEGISIISNRLCSKKVLIVLDDVDKLGQLQALVGGRDWFG 241
           QLQE LLYEIL    K++NLD GI+II NRLCSKKVLIVLDDVD+L QL+ALVGGRDWFG
Sbjct: 279 QLQESLLYEILMVHLKVINLDRGINIIRNRLCSKKVLIVLDDVDRLEQLEALVGGRDWFG 338

Query: 242 RDSKIIVTT-------SHGFDQMHDIQGLNPKNALELFSWHAFKKSQPLSNYLDLSKCAT 301
           + S+IIVTT       +HGFD++H+I GLN   A+ELFSWHAFKK+ P SNYLDLSK AT
Sbjct: 339 KGSRIIVTTRNKHLLFTHGFDEIHNILGLNEDKAIELFSWHAFKKNSPSSNYLDLSKRAT 398

Query: 302 TYCKGLPLALVVLGSFLCSRERSKWESILDEFENSQSKDIKDILRISFDGLEEKVKDIFL 361
            YC+G PLAL+VLGSFLC+R++ +W SILDEFENS +KDIKDIL++SFDGLEEK+KDIFL
Sbjct: 399 IYCEGHPLALIVLGSFLCTRDQVEWCSILDEFENSLNKDIKDILQLSFDGLEEKIKDIFL 458

Query: 362 DISCLFGREKVNYTKKMLSACHLNLDFGIIVLMDYSLITIVNDEVQMHDLIRQMGHKIVN 421
           DISCL   EKV Y K MLSACH NLDFGIIVLMD SL+TI ND+VQMHDLI+QMGHKIV 
Sbjct: 459 DISCLLVGEKVKYVKNMLSACHANLDFGIIVLMDLSLMTIENDKVQMHDLIKQMGHKIVC 518

Query: 422 GESSEPGKRSRLWL----------DSGTDAVKAIKLDLPKPTRLNVDPKAFRNMKNLRLL 475
           GES E GKRSRLWL          +SG DAVKAIKLD P PTRL+VD +AFR MKNLRLL
Sbjct: 519 GESLELGKRSRLWLVQDVWDVLVNNSGIDAVKAIKLDFPNPTRLDVDLQAFRKMKNLRLL 578

BLAST of Lag0006652 vs. ExPASy TrEMBL
Match: A0A1S4E0G6 (LOW QUALITY PROTEIN: disease resistance protein RPP4-like OS=Cucumis melo OX=3656 GN=LOC103499468 PE=4 SV=1)

HSP 1 Score: 686.4 bits (1770), Expect = 8.4e-194
Identity = 357/502 (71.12%), Postives = 409/502 (81.47%), Query Frame = 0

Query: 2   KSKGQLVLPVFYKVDPSEVRKQTGRFGEALAKHEAKFMSTKKIQTWKEALTIAADLSGWN 61
           KSKGQ+VLP+FYKVDPS++RKQ+G FGEALAKH+AKF +  K Q W+EALT AA+LSGW+
Sbjct: 121 KSKGQIVLPIFYKVDPSDIRKQSGTFGEALAKHQAKFQT--KTQIWREALTTAANLSGWD 180

Query: 62  LGTRKEADLIEDLVKEVLSVLNRTSTPLYVAKYPVGLDSQL-LGQLNFHS--------QY 121
           LGTRKEADLI DLVK VLS LNRT TPLYVAKYPVG+DS+L   +L  H+         Y
Sbjct: 181 LGTRKEADLIGDLVKNVLSTLNRTCTPLYVAKYPVGIDSKLEYMKLQSHNLFEKSNKFHY 240

Query: 122 GTQH---LSTGVYMLGIYGIGGIGKTTFAKALFNKIAHQFEGSCFLFNVRETSKQFNGLI 181
            TQH     TGVY++GIYGIGGIGKTT AKAL+NKIA QFEG CFL NVRE SKQFNGL 
Sbjct: 241 QTQHEYEFDTGVYLVGIYGIGGIGKTTLAKALYNKIASQFEGCCFLSNVREASKQFNGLA 300

Query: 182 QLQEKLLYEILKDDSKIVNLDEGISIISNRLCSKKVLIVLDDVDKLGQLQALVGGRDWFG 241
           QLQE LLYEIL  D K+VN+D GI+II NRLCSKKVLIVLDDVDKL QL+ LVGGRDWFG
Sbjct: 301 QLQESLLYEILTVDLKVVNIDRGINIIRNRLCSKKVLIVLDDVDKLEQLETLVGGRDWFG 360

Query: 242 RDSKIIVTT-------SHGFDQMHDIQGLNPKNALELFSWHAFKKSQPLSNYLDLSKCAT 301
           + S+I+VTT       SHGFD++H+I GLN   A+ELFSWHAFKK+ P SNY DLSK AT
Sbjct: 361 QGSRIMVTTRNKHLLSSHGFDEIHNILGLNEDKAIELFSWHAFKKNSPSSNYXDLSKRAT 420

Query: 302 TYCKGLPLALVVLGSFLCSRERSKWESILDEFENSQSKDIKDILRISFDGLEEKVKDIFL 361
           +YCKG PLALVVLGSFLC+R++ +W SILDEFENS +KDIKDIL++SFDGLE+K+KDIFL
Sbjct: 421 SYCKGHPLALVVLGSFLCTRDQVEWCSILDEFENSLNKDIKDILQLSFDGLEDKIKDIFL 480

Query: 362 DISCLFGREKVNYTKKMLSACHLNLDFGIIVLMDYSLITIVNDEVQMHDLIRQMGHKIVN 421
           DISCL   EKV Y K MLSACH+NLDFGIIVLMD SL+TI ND+VQM+DLI+QMGHKIV 
Sbjct: 481 DISCLLVGEKVKYVKNMLSACHVNLDFGIIVLMDLSLMTIENDKVQMYDLIKQMGHKIVC 540

Query: 422 GESSEPGKRSRLWL----------DSGTDAVKAIKLDLPKPTRLNVDPKAFRNMKNLRLL 475
           GES E GKRSRLWL          +SGTDAVKAIKLD P PT+L+VD +AFR MKNLRLL
Sbjct: 541 GESLELGKRSRLWLVRDVWEVLVNNSGTDAVKAIKLDFPNPTKLDVDLQAFRKMKNLRLL 600

BLAST of Lag0006652 vs. ExPASy TrEMBL
Match: A0A5A7TWK7 (TIR-NBS-LRR disease resistance protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold385G00340 PE=4 SV=1)

HSP 1 Score: 683.3 bits (1762), Expect = 7.1e-193
Identity = 357/502 (71.12%), Postives = 407/502 (81.08%), Query Frame = 0

Query: 2   KSKGQLVLPVFYKVDPSEVRKQTGRFGEALAKHEAKFMSTKKIQTWKEALTIAADLSGWN 61
           KSK Q+VLPVFYKVDPS++RKQ+G FGEALAKH+AKF +  KIQ W+EALT AA+LSGW+
Sbjct: 99  KSKDQIVLPVFYKVDPSDIRKQSGSFGEALAKHQAKFKT--KIQIWREALTTAANLSGWD 158

Query: 62  LGTRKEADLIEDLVKEVLSVLNRTSTPLYVAKYPVGLDSQL---------LGQLNFHSQY 121
           LGTRKEADLI D+VK+VLS LNRT  PLYVAKYPVG+DS+L         L + N    Y
Sbjct: 159 LGTRKEADLIGDIVKKVLSTLNRTCMPLYVAKYPVGIDSKLEYIKLRSHNLFEKNNKFHY 218

Query: 122 GTQH---LSTGVYMLGIYGIGGIGKTTFAKALFNKIAHQFEGSCFLFNVRETSKQFNGLI 181
            TQH     TG+YM+GIYGIGGIGKTT AKAL+NKIA QFEG CFL NVRE SKQFNGL 
Sbjct: 219 RTQHEYEFDTGIYMVGIYGIGGIGKTTLAKALYNKIASQFEGCCFLSNVREASKQFNGLA 278

Query: 182 QLQEKLLYEILKDDSKIVNLDEGISIISNRLCSKKVLIVLDDVDKLGQLQALVGGRDWFG 241
           QLQE LLYEIL  D K+VNLD GI+II NRLCSKKVLIVLDDVDKL QL+ALVGG DWFG
Sbjct: 279 QLQESLLYEILMVDLKVVNLDRGINIIRNRLCSKKVLIVLDDVDKLEQLEALVGGCDWFG 338

Query: 242 RDSKIIVTT-------SHGFDQMHDIQGLNPKNALELFSWHAFKKSQPLSNYLDLSKCAT 301
           + S+IIVTT       SHGFD++H+I GLN   A+ELFSWHAFKK++P SNYLDLSK AT
Sbjct: 339 KGSRIIVTTRNKHLLFSHGFDEIHNILGLNEDKAIELFSWHAFKKNRPSSNYLDLSKRAT 398

Query: 302 TYCKGLPLALVVLGSFLCSRERSKWESILDEFENSQSKDIKDILRISFDGLEEKVKDIFL 361
           +YCKG PLALVVLGSFLC R++++W SILDEFENS +KDIKDIL++SFDGLE+K+KDIFL
Sbjct: 399 SYCKGHPLALVVLGSFLCIRDQAEWCSILDEFENSLNKDIKDILQLSFDGLEDKIKDIFL 458

Query: 362 DISCLFGREKVNYTKKMLSACHLNLDFGIIVLMDYSLITIVNDEVQMHDLIRQMGHKIVN 421
           DISCL   EKV Y K MLSACH+NLDFG+IVLMD SLITI ND+VQMHDLI+QMG KIV 
Sbjct: 459 DISCLLVGEKVEYVKDMLSACHVNLDFGVIVLMDLSLITIENDKVQMHDLIKQMGQKIVC 518

Query: 422 GESSEPGKRSRLWL----------DSGTDAVKAIKLDLPKPTRLNVDPKAFRNMKNLRLL 475
           GES E GKRSRLWL          +SGTDA+KAIKLD P PTRL V+ +AFR MKNLRLL
Sbjct: 519 GESLELGKRSRLWLVHDVWEVLVNNSGTDAIKAIKLDFPNPTRLGVNSQAFRKMKNLRLL 578

BLAST of Lag0006652 vs. ExPASy TrEMBL
Match: E5GB33 (TIR-NBS-LRR disease resistance protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 681.0 bits (1756), Expect = 3.5e-192
Identity = 355/502 (70.72%), Postives = 406/502 (80.88%), Query Frame = 0

Query: 2   KSKGQLVLPVFYKVDPSEVRKQTGRFGEALAKHEAKFMSTKKIQTWKEALTIAADLSGWN 61
           KSK Q+VLPVFYKVDPS++RKQ+G FGEALAKH+AKF +  KIQ W+EALT AA+LSGW+
Sbjct: 99  KSKDQIVLPVFYKVDPSDIRKQSGSFGEALAKHQAKFKT--KIQIWREALTTAANLSGWD 158

Query: 62  LGTRKEADLIEDLVKEVLSVLNRTSTPLYVAKYPVGLDSQL---------LGQLNFHSQY 121
           LGTRKEADLI D+VK+VLS LNRT  PLYVAKYPVG+DS+L         + + N    Y
Sbjct: 159 LGTRKEADLIGDIVKKVLSTLNRTCMPLYVAKYPVGIDSKLEYIKLRSHNMFEKNNKFHY 218

Query: 122 GTQH---LSTGVYMLGIYGIGGIGKTTFAKALFNKIAHQFEGSCFLFNVRETSKQFNGLI 181
            TQH     TG+YM+GIYGIGGIGKTT AKAL+NKIA QFEG CFL NVRE SKQFNGL 
Sbjct: 219 RTQHEYEFDTGIYMVGIYGIGGIGKTTLAKALYNKIASQFEGCCFLSNVREASKQFNGLA 278

Query: 182 QLQEKLLYEILKDDSKIVNLDEGISIISNRLCSKKVLIVLDDVDKLGQLQALVGGRDWFG 241
           QLQE LLYEIL  D K+VNLD GI+II NRLCSKKVLIVLDDVDKL QL+ALVGG DWFG
Sbjct: 279 QLQESLLYEILMVDLKVVNLDRGINIIRNRLCSKKVLIVLDDVDKLEQLEALVGGCDWFG 338

Query: 242 RDSKIIVTT-------SHGFDQMHDIQGLNPKNALELFSWHAFKKSQPLSNYLDLSKCAT 301
           + S+IIVTT       SHGFD++H+I GLN   A+ELFSWHAFKK++P SNYLDLSK AT
Sbjct: 339 KGSRIIVTTRNKHLLFSHGFDEIHNILGLNEDKAIELFSWHAFKKNRPSSNYLDLSKRAT 398

Query: 302 TYCKGLPLALVVLGSFLCSRERSKWESILDEFENSQSKDIKDILRISFDGLEEKVKDIFL 361
           +YCKG PLALVVLGSFLC R++++W SILDEFENS +KDIKDIL++SFDGLE+K+KDIFL
Sbjct: 399 SYCKGHPLALVVLGSFLCIRDQAEWCSILDEFENSLNKDIKDILQLSFDGLEDKIKDIFL 458

Query: 362 DISCLFGREKVNYTKKMLSACHLNLDFGIIVLMDYSLITIVNDEVQMHDLIRQMGHKIVN 421
           DISCL   EKV Y K ML ACH+NLDFG+IVLMD SLITI ND+VQMHDLI+QMG KIV 
Sbjct: 459 DISCLLVGEKVEYVKDMLGACHVNLDFGVIVLMDLSLITIENDKVQMHDLIKQMGQKIVC 518

Query: 422 GESSEPGKRSRLWL----------DSGTDAVKAIKLDLPKPTRLNVDPKAFRNMKNLRLL 475
           GES E GKRSRLWL          +SGTDA+KAIKLD P PTRL V+ +AFR MKNLRLL
Sbjct: 519 GESLELGKRSRLWLVQDVWEVLVNNSGTDAIKAIKLDFPNPTRLGVNSQAFRKMKNLRLL 578

BLAST of Lag0006652 vs. ExPASy TrEMBL
Match: A0A1S4E027 (TMV resistance protein N-like OS=Cucumis melo OX=3656 GN=LOC103499448 PE=4 SV=1)

HSP 1 Score: 681.0 bits (1756), Expect = 3.5e-192
Identity = 355/502 (70.72%), Postives = 406/502 (80.88%), Query Frame = 0

Query: 2   KSKGQLVLPVFYKVDPSEVRKQTGRFGEALAKHEAKFMSTKKIQTWKEALTIAADLSGWN 61
           KSK Q+VLPVFYKVDPS++RKQ+G FGEALAKH+AKF +  KIQ W+EALT AA+LSGW+
Sbjct: 99  KSKDQIVLPVFYKVDPSDIRKQSGSFGEALAKHQAKFKT--KIQIWREALTTAANLSGWD 158

Query: 62  LGTRKEADLIEDLVKEVLSVLNRTSTPLYVAKYPVGLDSQL---------LGQLNFHSQY 121
           LGTRKEADLI D+VK+VLS LNRT  PLYVAKYPVG+DS+L         + + N    Y
Sbjct: 159 LGTRKEADLIGDIVKKVLSTLNRTCMPLYVAKYPVGIDSKLEYIKLRSHNMFEKNNKFHY 218

Query: 122 GTQH---LSTGVYMLGIYGIGGIGKTTFAKALFNKIAHQFEGSCFLFNVRETSKQFNGLI 181
            TQH     TG+YM+GIYGIGGIGKTT AKAL+NKIA QFEG CFL NVRE SKQFNGL 
Sbjct: 219 RTQHEYEFDTGIYMVGIYGIGGIGKTTLAKALYNKIASQFEGCCFLSNVREASKQFNGLA 278

Query: 182 QLQEKLLYEILKDDSKIVNLDEGISIISNRLCSKKVLIVLDDVDKLGQLQALVGGRDWFG 241
           QLQE LLYEIL  D K+VNLD GI+II NRLCSKKVLIVLDDVDKL QL+ALVGG DWFG
Sbjct: 279 QLQESLLYEILMVDLKVVNLDRGINIIRNRLCSKKVLIVLDDVDKLEQLEALVGGCDWFG 338

Query: 242 RDSKIIVTT-------SHGFDQMHDIQGLNPKNALELFSWHAFKKSQPLSNYLDLSKCAT 301
           + S+IIVTT       SHGFD++H+I GLN   A+ELFSWHAFKK++P SNYLDLSK AT
Sbjct: 339 KGSRIIVTTRNKHLLFSHGFDEIHNILGLNEDKAIELFSWHAFKKNRPSSNYLDLSKRAT 398

Query: 302 TYCKGLPLALVVLGSFLCSRERSKWESILDEFENSQSKDIKDILRISFDGLEEKVKDIFL 361
           +YCKG PLALVVLGSFLC R++++W SILDEFENS +KDIKDIL++SFDGLE+K+KDIFL
Sbjct: 399 SYCKGHPLALVVLGSFLCIRDQAEWCSILDEFENSLNKDIKDILQLSFDGLEDKIKDIFL 458

Query: 362 DISCLFGREKVNYTKKMLSACHLNLDFGIIVLMDYSLITIVNDEVQMHDLIRQMGHKIVN 421
           DISCL   EKV Y K ML ACH+NLDFG+IVLMD SLITI ND+VQMHDLI+QMG KIV 
Sbjct: 459 DISCLLVGEKVEYVKDMLGACHVNLDFGVIVLMDLSLITIENDKVQMHDLIKQMGQKIVC 518

Query: 422 GESSEPGKRSRLWL----------DSGTDAVKAIKLDLPKPTRLNVDPKAFRNMKNLRLL 475
           GES E GKRSRLWL          +SGTDA+KAIKLD P PTRL V+ +AFR MKNLRLL
Sbjct: 519 GESLELGKRSRLWLVQDVWEVLVNNSGTDAIKAIKLDFPNPTRLGVNSQAFRKMKNLRLL 578

BLAST of Lag0006652 vs. TAIR 10
Match: AT5G36930.1 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 306.6 bits (784), Expect = 3.5e-83
Identity = 193/493 (39.15%), Postives = 278/493 (56.39%), Query Frame = 0

Query: 7   LVLPVFYKVDPSEVRKQTGRFGEALAKHEAKFMSTKKIQTWKEALTIAADLSGWNLGTRK 66
           +V P+F  VDPS++R Q G + ++ +KH+       K++ W+EALT  A++SGW++  R 
Sbjct: 103 MVFPIFLYVDPSDIRWQQGSYAKSFSKHKNSH-PLNKLKDWREALTKVANISGWDIKNRN 162

Query: 67  EADLIEDLVKEVLSVLNRTSTPLYVAKYPVGLDSQLLGQLNFHSQYGTQHLST------- 126
           EA+ I D+ +E+L  L      L+V  Y VGL S+L            QH+S+       
Sbjct: 163 EAECIADITREILKRL--PCQYLHVPSYAVGLRSRL------------QHISSLLSIGSD 222

Query: 127 GVYMLGIYGIGGIGKTTFAKALFNKIAHQFEGSCFLFNVRETSKQFNGLIQLQEKLLYEI 186
           GV ++ IYG+GGIGKTT AK  FN+ +H FEGS FL N RE SK+  G   LQ +LL +I
Sbjct: 223 GVRVIVIYGMGGIGKTTLAKVAFNEFSHLFEGSSFLENFREYSKKPEGRTHLQHQLLSDI 282

Query: 187 L-KDDSKIVNLDEGISIISNRLCSKKVLIVLDDVDKLGQLQALVGGRDWFGRDSKIIVTT 246
           L ++D +   LD     +  R  SK+VL+V+DDVD + QL +    RD FG  S+II+TT
Sbjct: 283 LRRNDIEFKGLDHA---VKERFRSKRVLLVVDDVDDVHQLNSAAIDRDCFGHGSRIIITT 342

Query: 247 --SHGFDQMHDIQGLNPK-----NALELFSWHAFKKSQPLSNYLDLSKCATTYCKGLPLA 306
              H   Q+      +PK      +LELFSWHAF+ S+P   +L  S+   TYC GLPLA
Sbjct: 343 RNMHLLKQLRAEGSYSPKELDGDESLELFSWHAFRTSEPPKEFLQHSEEVVTYCAGLPLA 402

Query: 307 LVVLGSFLCSRERSKWESILDEFENSQSKDIKDILRISFDGLEEKVKDIFLDISCLFGRE 366
           + VLG+FL  R   +WES L   +   + +I+  L+ISF+ L  + KD+FLDI+C F   
Sbjct: 403 VEVLGAFLIERSIREWESTLKLLKRIPNDNIQAKLQISFNALTIEQKDVFLDIACFFIGV 462

Query: 367 KVNYTKKMLSACHLNLDFGIIVLMDYSLITIVNDEVQMHDLIRQMGHKIVNGESSEP-GK 426
              Y   +L  C+L  D  + +LM+  LITI  + + MHDL+R MG +IV   S +  G+
Sbjct: 463 DSYYVACILDGCNLYPDIVLSLLMERCLITISGNNIMMHDLLRDMGRQIVREISPKKCGE 522

Query: 427 RSRLW----------LDSGTDAVKAIKLDLPKPTRLNVDPKAFRNMKNLRLLIVRNATFC 474
           RSRLW            SGT+A++ + L          + +AF  M+ LRLL +R     
Sbjct: 523 RSRLWSHNDVVGVLKKKSGTNAIEGLSLKADVMDFQYFEVEAFAKMQELRLLELRYVDLN 577

BLAST of Lag0006652 vs. TAIR 10
Match: AT5G36930.2 (Disease resistance protein (TIR-NBS-LRR class) family )

HSP 1 Score: 306.6 bits (784), Expect = 3.5e-83
Identity = 193/493 (39.15%), Postives = 278/493 (56.39%), Query Frame = 0

Query: 7   LVLPVFYKVDPSEVRKQTGRFGEALAKHEAKFMSTKKIQTWKEALTIAADLSGWNLGTRK 66
           +V P+F  VDPS++R Q G + ++ +KH+       K++ W+EALT  A++SGW++  R 
Sbjct: 106 MVFPIFLYVDPSDIRWQQGSYAKSFSKHKNSH-PLNKLKDWREALTKVANISGWDIKNRN 165

Query: 67  EADLIEDLVKEVLSVLNRTSTPLYVAKYPVGLDSQLLGQLNFHSQYGTQHLST------- 126
           EA+ I D+ +E+L  L      L+V  Y VGL S+L            QH+S+       
Sbjct: 166 EAECIADITREILKRL--PCQYLHVPSYAVGLRSRL------------QHISSLLSIGSD 225

Query: 127 GVYMLGIYGIGGIGKTTFAKALFNKIAHQFEGSCFLFNVRETSKQFNGLIQLQEKLLYEI 186
           GV ++ IYG+GGIGKTT AK  FN+ +H FEGS FL N RE SK+  G   LQ +LL +I
Sbjct: 226 GVRVIVIYGMGGIGKTTLAKVAFNEFSHLFEGSSFLENFREYSKKPEGRTHLQHQLLSDI 285

Query: 187 L-KDDSKIVNLDEGISIISNRLCSKKVLIVLDDVDKLGQLQALVGGRDWFGRDSKIIVTT 246
           L ++D +   LD     +  R  SK+VL+V+DDVD + QL +    RD FG  S+II+TT
Sbjct: 286 LRRNDIEFKGLDHA---VKERFRSKRVLLVVDDVDDVHQLNSAAIDRDCFGHGSRIIITT 345

Query: 247 --SHGFDQMHDIQGLNPK-----NALELFSWHAFKKSQPLSNYLDLSKCATTYCKGLPLA 306
              H   Q+      +PK      +LELFSWHAF+ S+P   +L  S+   TYC GLPLA
Sbjct: 346 RNMHLLKQLRAEGSYSPKELDGDESLELFSWHAFRTSEPPKEFLQHSEEVVTYCAGLPLA 405

Query: 307 LVVLGSFLCSRERSKWESILDEFENSQSKDIKDILRISFDGLEEKVKDIFLDISCLFGRE 366
           + VLG+FL  R   +WES L   +   + +I+  L+ISF+ L  + KD+FLDI+C F   
Sbjct: 406 VEVLGAFLIERSIREWESTLKLLKRIPNDNIQAKLQISFNALTIEQKDVFLDIACFFIGV 465

Query: 367 KVNYTKKMLSACHLNLDFGIIVLMDYSLITIVNDEVQMHDLIRQMGHKIVNGESSEP-GK 426
              Y   +L  C+L  D  + +LM+  LITI  + + MHDL+R MG +IV   S +  G+
Sbjct: 466 DSYYVACILDGCNLYPDIVLSLLMERCLITISGNNIMMHDLLRDMGRQIVREISPKKCGE 525

Query: 427 RSRLW----------LDSGTDAVKAIKLDLPKPTRLNVDPKAFRNMKNLRLLIVRNATFC 474
           RSRLW            SGT+A++ + L          + +AF  M+ LRLL +R     
Sbjct: 526 RSRLWSHNDVVGVLKKKSGTNAIEGLSLKADVMDFQYFEVEAFAKMQELRLLELRYVDLN 580

BLAST of Lag0006652 vs. TAIR 10
Match: AT1G27170.1 (transmembrane receptors;ATP binding )

HSP 1 Score: 291.2 bits (744), Expect = 1.5e-78
Identity = 188/537 (35.01%), Postives = 294/537 (54.75%), Query Frame = 0

Query: 8   VLPVFYKVDPSEVRKQTGRFGEALAKHEAKFMSTK-KIQTWKEALTIAADLSGWNLG-TR 67
           +LP+FY VDPS VRKQ+    +   +H+ +F   K K+Q W+EALT+  +L+G+      
Sbjct: 102 ILPIFYHVDPSHVRKQSDHIKKDFEEHQVRFSEEKEKVQEWREALTLVGNLAGYVCDKDS 161

Query: 68  KEADLIEDLVKEVLSVLNRTSTPLYVAKYPVGLDS---QLLGQLNFHSQYGTQHLSTGVY 127
           K+ D+IE +VK VL+ L  ++TP  V ++ VGL+S    L G ++  S       S+GV 
Sbjct: 162 KDDDMIELVVKRVLAEL--SNTPEKVGEFIVGLESPLKDLTGLIDTES-------SSGVQ 221

Query: 128 MLGIYGIGGIGKTTFAKALFNKIAHQFEGSCFLFNVRETSKQFNGLIQLQEKLLYEILKD 187
           +LG+YG+GGIGKTT AKA +NKI   FE   F+ ++RE S   NGL+ LQ+ L+ E+ + 
Sbjct: 222 VLGLYGMGGIGKTTLAKAFYNKIVGNFEQRAFISDIRERSSAENGLVTLQKTLIKELFRL 281

Query: 188 DSKIVNLDEGISIISNRLCSKKVLIVLDDVDKLGQLQALVGGRDWFGRDSKIIVTTSH-- 247
             +I ++  G+  I   +  KK+++VLDDVD + Q+ ALVG   W+G+ + I++TT    
Sbjct: 282 VPEIEDVSIGLEKIKANVHEKKIIVVLDDVDHIDQVHALVGETRWYGQGTLIVITTRDSE 341

Query: 248 -----GFDQMHDIQGLNPKNALELFSWHAFKKSQPLSNYLDLSKCATTYCKGLPLALVVL 307
                  +Q ++++ L    AL+LFS+H+ +K +P  N L LSK        LPLA+ V 
Sbjct: 342 ILSKLSVNQQYEVKCLTEPQALKLFSYHSLRKEEPTKNLLALSKKIVQISGLLPLAVEVF 401

Query: 308 GSFLCSRERSK-WESILDEFENSQSKDIKDILRISFDGLEEKVKDIFLDISCLFGREKVN 367
           GS L  ++  K W++ LD+ + +Q  +++D+L +SF  L+++ K +FLDI+CLF + ++ 
Sbjct: 402 GSLLYDKKEEKDWQTQLDKLKKTQPGNLQDVLELSFKSLDDEEKKVFLDIACLFLKMEIK 461

Query: 368 YTKK--MLSACHLNLDFGIIVLMDYSLITIV-NDEVQMHDLIRQMGHKIVNGESSE-PGK 427
             +   +L  C LN +  + VL   SL+ I+ ND + MHD IR MG ++V  ES E PG 
Sbjct: 462 KDEVVIVLKGCGLNAEAALSVLRQKSLVKILANDTLWMHDQIRDMGRQMVLKESREDPGL 521

Query: 428 RSRLW--------LDS--GTDAVKAIKLDL------------------------------ 475
           RSRLW        L++  GT +++ I LD                               
Sbjct: 522 RSRLWDRGEIMTVLNNMKGTSSIRGIVLDFKKKFARDPTADEIVSRNLRNNPGIYSVFNY 581

BLAST of Lag0006652 vs. TAIR 10
Match: AT1G27170.2 (transmembrane receptors;ATP binding )

HSP 1 Score: 288.1 bits (736), Expect = 1.3e-77
Identity = 189/541 (34.94%), Postives = 295/541 (54.53%), Query Frame = 0

Query: 3   SKGQLVLPVFYKVDPSEVRKQTGRFGEALAKHEAKFMSTKKIQTWKEALTIAADLSGWNL 62
           S G+LVLP+FY+V+P  +RKQ G +     +H  +F S +KIQ W+ AL I  ++ G+  
Sbjct: 99  SLGRLVLPIFYEVEPCMLRKQNGPYEMDFEEHSKRF-SEEKIQRWRRALNIIGNIPGFVY 158

Query: 63  G-TRKEADLIEDLVKEVLSVLNRTSTPLYVAKYPVGLDS---QLLGQLNFHSQYGTQHLS 122
               K+ D+IE +VK VL+ L  ++TP  V ++ VGL+S    L G ++  S       S
Sbjct: 159 SKDSKDDDMIELVVKRVLAEL--SNTPEKVGEFIVGLESPLKDLTGLIDTES-------S 218

Query: 123 TGVYMLGIYGIGGIGKTTFAKALFNKIAHQFEGSCFLFNVRETSKQFNGLIQLQEKLLYE 182
           +GV +LG+YG+GGIGKTT AKA +NKI   FE   F+ ++RE S   NGL+ LQ+ L+ E
Sbjct: 219 SGVQVLGLYGMGGIGKTTLAKAFYNKIVGNFEQRAFISDIRERSSAENGLVTLQKTLIKE 278

Query: 183 ILKDDSKIVNLDEGISIISNRLCSKKVLIVLDDVDKLGQLQALVGGRDWFGRDSKIIVTT 242
           + +   +I ++  G+  I   +  KK+++VLDDVD + Q+ ALVG   W+G+ + I++TT
Sbjct: 279 LFRLVPEIEDVSIGLEKIKANVHEKKIIVVLDDVDHIDQVHALVGETRWYGQGTLIVITT 338

Query: 243 SH-------GFDQMHDIQGLNPKNALELFSWHAFKKSQPLSNYLDLSKCATTYCKGLPLA 302
                      +Q ++++ L    AL+LFS+H+ +K +P  N L LSK        LPLA
Sbjct: 339 RDSEILSKLSVNQQYEVKCLTEPQALKLFSYHSLRKEEPTKNLLALSKKIVQISGLLPLA 398

Query: 303 LVVLGSFLCSRERSK-WESILDEFENSQSKDIKDILRISFDGLEEKVKDIFLDISCLFGR 362
           + V GS L  ++  K W++ LD+ + +Q  +++D+L +SF  L+++ K +FLDI+CLF +
Sbjct: 399 VEVFGSLLYDKKEEKDWQTQLDKLKKTQPGNLQDVLELSFKSLDDEEKKVFLDIACLFLK 458

Query: 363 EKVNYTKK--MLSACHLNLDFGIIVLMDYSLITIV-NDEVQMHDLIRQMGHKIVNGESSE 422
            ++   +   +L  C LN +  + VL   SL+ I+ ND + MHD IR MG ++V  ES E
Sbjct: 459 MEIKKDEVVIVLKGCGLNAEAALSVLRQKSLVKILANDTLWMHDQIRDMGRQMVLKESRE 518

Query: 423 -PGKRSRLW--------LDS--GTDAVKAIKLDL-------------------------- 475
            PG RSRLW        L++  GT +++ I LD                           
Sbjct: 519 DPGLRSRLWDRGEIMTVLNNMKGTSSIRGIVLDFKKKFARDPTADEIVSRNLRNNPGIYS 578

BLAST of Lag0006652 vs. TAIR 10
Match: AT5G17680.1 (disease resistance protein (TIR-NBS-LRR class), putative )

HSP 1 Score: 279.3 bits (713), Expect = 5.9e-75
Identity = 182/504 (36.11%), Postives = 283/504 (56.15%), Query Frame = 0

Query: 1   MKSKGQLVLPVFYKVDPSEVRKQTGRFGEALAKHEAKFMSTKKIQTWKEALTIAADLSGW 60
           M+     ++P+FY+VDPS+VR+Q G FGE +  H  K    +K+  WKEAL   A +SG 
Sbjct: 97  MECNKDTIVPIFYEVDPSDVRRQRGSFGEDVESHSDK----EKVGKWKEALKKLAAISGE 156

Query: 61  NLGTRKEADLIEDLVKEVLSVLNRTSTPLYVAKYPVGLDSQLLGQLNFHSQYGTQHLS-- 120
           +     ++ LI+ +VK++   L  TS            DS+ L  ++ H  +    +S  
Sbjct: 157 DSRNWDDSKLIKKIVKDISDKLVSTSWD----------DSKGLIGMSSHMDFLQSMISIV 216

Query: 121 -TGVYMLGIYGIGGIGKTTFAKALFNKIAHQFEGSCFLFNVRETSKQFNGLIQLQEKLLY 180
              V MLGI+G+GG+GKTT AK L+N+++ QF+  CF+ NV+E   ++ G+ +LQ + L 
Sbjct: 217 DKDVRMLGIWGMGGVGKTTIAKYLYNQLSGQFQVHCFMENVKEVCNRY-GVRRLQVEFLC 276

Query: 181 EILKD-DSKIVNLDEGISIISNRLCSKKVLIVLDDVDKLGQLQALVGGRDWFGRDSKIIV 240
            + ++ D +  +     +II  R   K V IVLDDVD+  QL  LV    WFG  S+IIV
Sbjct: 277 RMFQERDKEAWSSVSCCNIIKERFRHKMVFIVLDDVDRSEQLNELVKETGWFGPGSRIIV 336

Query: 241 TT-------SHGFDQMHDIQGLNPKNALELFSWHAFKKSQPLSN-YLDLSKCATTYCKGL 300
           TT       SHG + ++ ++ L  K AL+LF  +AF++   L + + +LS  A  Y  GL
Sbjct: 337 TTRDRHLLLSHGINLVYKVKCLPKKEALQLFCNYAFREEIILPHGFEELSVQAVNYASGL 396

Query: 301 PLALVVLGSFLCSRERSKWESILDEFENSQSKDIKDILRISFDGLEEKVKDIFLDISCLF 360
           PLAL VLGSFL  R + +WES L   +     DI ++LR+S+DGL+E+ K IFL ISC +
Sbjct: 397 PLALRVLGSFLYRRSQIEWESTLARLKTYPHSDIMEVLRVSYDGLDEQEKAIFLYISCFY 456

Query: 361 GREKVNYTKKMLSACHLNLDFGIIVLMDYSLITIVNDEVQMHDLIRQMGHKIVNGES-SE 420
             ++V+Y +K+L  C    + GI +L + SLI   N  V++HDL+ QMG ++V  ++ + 
Sbjct: 457 NMKQVDYVRKLLDLCGYAAEIGITILTEKSLIVESNGCVKIHDLLEQMGRELVRQQAVNN 516

Query: 421 PGKRSRLW----------LDSGTDAVKAIKLDLPKPTRLNVDPKAFRNMKNLRLLIVRNA 474
           P +R  LW           +SGT  V+ I L+L + + +    +AF  + NL+LL   + 
Sbjct: 517 PAQRLLLWDPEDICHLLSENSGTQLVEGISLNLSEISEVFASDRAFEGLSNLKLLNFYDL 576

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038877735.14.0e-19874.44disease resistance protein RPS6-like, partial [Benincasa hispida][more]
TYK18296.11.3e-19371.31disease resistance protein RPP4-like [Cucumis melo var. makuwa][more]
XP_016901470.11.7e-19371.12PREDICTED: LOW QUALITY PROTEIN: disease resistance protein RPP4-like [Cucumis me... [more]
KAA0047973.11.5e-19271.12TIR-NBS-LRR disease resistance protein [Cucumis melo var. makuwa][more]
ADN33675.17.3e-19270.72TIR-NBS-LRR disease resistance protein [Cucumis melo subsp. melo][more]
Match NameE-valueIdentityDescription
V9M2S54.9e-8240.08Disease resistance protein RPV1 OS=Vitis rotundifolia OX=103349 GN=RPV1 PE=1 SV=... [more]
V9M3981.7e-7939.67Disease resistance protein RUN1 OS=Vitis rotundifolia OX=103349 GN=RUN1 PE=1 SV=... [more]
Q403924.3e-7837.88TMV resistance protein N OS=Nicotiana glutinosa OX=35889 GN=N PE=1 SV=1[more]
A0A290U7C45.6e-7035.73Disease resistance protein Roq1 OS=Nicotiana benthamiana OX=4100 GN=ROQ1 PE=1 SV... [more]
Q402531.2e-6935.63Disease resistance protein L6 OS=Linum usitatissimum OX=4006 GN=L6 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A5D3D4326.4e-19471.31Disease resistance protein RPP4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A1S4E0G68.4e-19471.12LOW QUALITY PROTEIN: disease resistance protein RPP4-like OS=Cucumis melo OX=365... [more]
A0A5A7TWK77.1e-19371.12TIR-NBS-LRR disease resistance protein OS=Cucumis melo var. makuwa OX=1194695 GN... [more]
E5GB333.5e-19270.72TIR-NBS-LRR disease resistance protein OS=Cucumis melo subsp. melo OX=412675 PE=... [more]
A0A1S4E0273.5e-19270.72TMV resistance protein N-like OS=Cucumis melo OX=3656 GN=LOC103499448 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G36930.13.5e-8339.15Disease resistance protein (TIR-NBS-LRR class) family [more]
AT5G36930.23.5e-8339.15Disease resistance protein (TIR-NBS-LRR class) family [more]
AT1G27170.11.5e-7835.01transmembrane receptors;ATP binding [more]
AT1G27170.21.3e-7734.94transmembrane receptors;ATP binding [more]
AT5G17680.15.9e-7536.11disease resistance protein (TIR-NBS-LRR class), putative [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePRINTSPR00364DISEASERSISTcoord: 123..138
score: 57.66
coord: 444..460
score: 20.88
coord: 199..213
score: 56.5
coord: 285..299
score: 56.17
NoneNo IPR availablePANTHERPTHR11017:SF422RESISTANCE PROTEIN (TIR-NBS-LRR CLASS), PUTATIVE-RELATEDcoord: 8..472
IPR035897Toll/interleukin-1 receptor homology (TIR) domain superfamilyGENE3D3.40.50.10140coord: 1..88
e-value: 1.7E-25
score: 91.7
IPR035897Toll/interleukin-1 receptor homology (TIR) domain superfamilySUPERFAMILY52200Toll/Interleukin receptor TIR domaincoord: 2..60
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 107..246
e-value: 3.5E-26
score: 93.8
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 118..344
IPR000157Toll/interleukin-1 receptor homology (TIR) domainPFAMPF01582TIRcoord: 3..85
e-value: 3.2E-12
score: 46.6
IPR000157Toll/interleukin-1 receptor homology (TIR) domainPROSITEPS50104TIRcoord: 1..82
score: 13.143473
IPR042197Apoptotic protease-activating factors, helical domainGENE3D1.10.8.430coord: 250..334
e-value: 3.3E-18
score: 67.0
IPR002182NB-ARCPFAMPF00931NB-ARCcoord: 120..328
e-value: 1.9E-24
score: 86.2
IPR044974Disease resistance protein, plantsPANTHERPTHR11017LEUCINE-RICH REPEAT-CONTAINING PROTEINcoord: 8..472
IPR036390Winged helix DNA-binding domain superfamilySUPERFAMILY46785"Winged helix" DNA-binding domaincoord: 321..405

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0006652.1Lag0006652.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
biological_process GO:0007165 signal transduction
molecular_function GO:0043531 ADP binding
molecular_function GO:0003953 NAD+ nucleosidase activity