Lag0006631 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0006631
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	kunitz trypsin inhibitor 1-like
Location	chr6: 44343609 .. 44344301 (+)
RNA-Seq Expression	Lag0006631
Synteny	Lag0006631

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGATGTTGCATAAGCCCCTTGCCATCTTCAGCTGCCTATGCCTCTTCATGGTCATATCCTCTACTGCCCAATCCGATGGTCCTCCGGTGCTCGACACCGAGGGCCGATCTCTACAGCGCGGCGTCGAGTACTTCATCAAGCCTGCCATCACCGATGTTGCTGGCAATCTCACCCTAGTAACCCGCAAAGGTGATCCGTGCCCGTTCAACGTTGGCCAAGTACCACTTCTTTCACAAGATATAGGTATATTAAACTATTACGATTAAACTCTAGAACTAAATGGATACGTTATGATATATTGAAAATTTGGACTTTTGTATGTATTCTTAACAATAGGTTTCGCGGTCACCTTCACACCCTATATAGAGGGTGAAGATACGATTAGAGAAGGCAGGGATTTGAAGGTCGTGTTTCAGGCGTTCACAACTTGCATAACGTCGACTGAATGGAGAGTAGGGGAGACGGAGCCTACCACCGGAAGGAGGTTTGTGGAGACCGGGAGCGACGACACTGCCACCGGGTACTTCAGGATCGACAATAGCGACGGGATTTATAATATAGGATGGTGTCCCTCGGATGTCCCCATTAGAGGGAGGCCAAGATGTGGAAGTGCTGGAATTTTGATTGAGAAAGGTGTGAGGTTGTTGGCTTTGGATGGCTCTGCGTTTCCTTTTGAGTTTGTGAGAGCTTGA

mRNA sequence

ATGATGTTGCATAAGCCCCTTGCCATCTTCAGCTGCCTATGCCTCTTCATGGTCATATCCTCTACTGCCCAATCCGATGGTCCTCCGGTGCTCGACACCGAGGGCCGATCTCTACAGCGCGGCGTCGAGTACTTCATCAAGCCTGCCATCACCGATGTTGCTGGCAATCTCACCCTAGTAACCCGCAAAGGTGATCCGTGCCCGTTCAACGTTGGCCAAGTACCACTTCTTTCACAAGATATAGGTTTCGCGGTCACCTTCACACCCTATATAGAGGGTGAAGATACGATTAGAGAAGGCAGGGATTTGAAGGTCGTGTTTCAGGCGTTCACAACTTGCATAACGTCGACTGAATGGAGAGTAGGGGAGACGGAGCCTACCACCGGAAGGAGGTTTGTGGAGACCGGGAGCGACGACACTGCCACCGGGTACTTCAGGATCGACAATAGCGACGGGATTTATAATATAGGATGGTGTCCCTCGGATGTCCCCATTAGAGGGAGGCCAAGATGTGGAAGTGCTGGAATTTTGATTGAGAAAGGTGTGAGGTTGTTGGCTTTGGATGGCTCTGCGTTTCCTTTTGAGTTTGTGAGAGCTTGA

Coding sequence (CDS)

ATGATGTTGCATAAGCCCCTTGCCATCTTCAGCTGCCTATGCCTCTTCATGGTCATATCCTCTACTGCCCAATCCGATGGTCCTCCGGTGCTCGACACCGAGGGCCGATCTCTACAGCGCGGCGTCGAGTACTTCATCAAGCCTGCCATCACCGATGTTGCTGGCAATCTCACCCTAGTAACCCGCAAAGGTGATCCGTGCCCGTTCAACGTTGGCCAAGTACCACTTCTTTCACAAGATATAGGTTTCGCGGTCACCTTCACACCCTATATAGAGGGTGAAGATACGATTAGAGAAGGCAGGGATTTGAAGGTCGTGTTTCAGGCGTTCACAACTTGCATAACGTCGACTGAATGGAGAGTAGGGGAGACGGAGCCTACCACCGGAAGGAGGTTTGTGGAGACCGGGAGCGACGACACTGCCACCGGGTACTTCAGGATCGACAATAGCGACGGGATTTATAATATAGGATGGTGTCCCTCGGATGTCCCCATTAGAGGGAGGCCAAGATGTGGAAGTGCTGGAATTTTGATTGAGAAAGGTGTGAGGTTGTTGGCTTTGGATGGCTCTGCGTTTCCTTTTGAGTTTGTGAGAGCTTGA

Protein sequence

MMLHKPLAIFSCLCLFMVISSTAQSDGPPVLDTEGRSLQRGVEYFIKPAITDVAGNLTLVTRKGDPCPFNVGQVPLLSQDIGFAVTFTPYIEGEDTIREGRDLKVVFQAFTTCITSTEWRVGETEPTTGRRFVETGSDDTATGYFRIDNSDGIYNIGWCPSDVPIRGRPRCGSAGILIEKGVRLLALDGSAFPFEFVRA

Homology

[Close]

BLAST of Lag0006631 vs. NCBI nr
Match: XP_038888505.1 (kunitz type trypsin inhibitor 111-like [Benincasa hispida])

HSP 1 Score: 356.7 bits (914), Expect = 1.3e-94
Identity = 171/199 (85.93%), Postives = 180/199 (90.45%), Query Frame = 0


Query: 1   MMLHKPLAIFSCLCLFMVISSTAQSDGPPVLDTEGRSLQRGVEYFIKPAITDVAGNLTLV 60
           MM HK LAIFS LC FM ISS AQ DGPPVLDTEGR LQR VEYFIKPAITDVAGNLTL+
Sbjct: 2   MMFHKSLAIFSSLCFFMAISSIAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTLI 61

Query: 61  TRKGDPCPFNVGQVPLLSQDIGFAVTFTPYIEGEDTIREGRDLKVVFQAFTTCITSTEWR 120
           TRKGD CPF VGQVPLLSQDIGF VTFTPY+ GEDTIREGRDLKVVFQAFT CITST+W+
Sbjct: 62  TRKGDQCPFYVGQVPLLSQDIGFGVTFTPYLAGEDTIREGRDLKVVFQAFTICITSTQWK 121

Query: 121 VGETEPTTGRRFVETGSDDTATGYFRIDNSD-GIYNIGWCPSDVPIRGRPRCGSAGILIE 180
           VGE +P TGRRFV+ G+DDTATGYFRID SD G+YNIGWCPSDVPIRGRPRCGSAGILIE
Sbjct: 122 VGEADPITGRRFVKLGNDDTATGYFRIDKSDLGVYNIGWCPSDVPIRGRPRCGSAGILIE 181

Query: 181 KGVRLLALDGSAFPFEFVR 199
           KG+RLLALDGSAFPFEFVR
Sbjct: 182 KGLRLLALDGSAFPFEFVR 200

[Close]

BLAST of Lag0006631 vs. NCBI nr
Match: XP_022938134.1 (alpha-amylase/subtilisin inhibitor-like [Cucurbita moschata])

HSP 1 Score: 336.7 bits (862), Expect = 1.4e-88
Identity = 165/200 (82.50%), Postives = 176/200 (88.00%), Query Frame = 0


Query: 1   MMLHKPLAIFSCLCLFMVISSTAQSDGPPVLDTEGRSLQRGVEYFIKPAITDVAGNLTLV 60
           MML K L  FS LCL M +SS AQS  PPVLDTEGR LQ  VEYFIKPA+TDVAGNLTL+
Sbjct: 1   MMLCKSLVAFSYLCLVMAVSSIAQS--PPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLL 60

Query: 61  TRKGDPCPFNVGQVPLLSQDIGFAVTFTPYIEGEDTIREGRDLKVVFQAFTTCITSTEWR 120
           TRKGD CPF+VGQVPL SQ+IGF VTFTPY+ GEDTIREGRDLKVVFQAF+ CITST WR
Sbjct: 61  TRKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWR 120

Query: 121 VGETEPTTGRRFVETGSDDTATGYFRIDNSD-GIYNIGWCPSDVPIRGRPRCGSAGILIE 180
           VGET+PTTGRRFVETG DDTATGYFRID  + G+YNIGWCPSDVPIRGRPRCG AGILIE
Sbjct: 121 VGETDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIE 180

Query: 181 KGVRLLALDGSAFPFEFVRA 200
           KG+RLLALDGSAFPFEFVRA
Sbjct: 181 KGLRLLALDGSAFPFEFVRA 198

[Close]

BLAST of Lag0006631 vs. NCBI nr
Match: XP_022150807.1 (miraculin-like [Momordica charantia])

HSP 1 Score: 336.3 bits (861), Expect = 1.8e-88
Identity = 163/200 (81.50%), Postives = 178/200 (89.00%), Query Frame = 0


Query: 1   MMLHKPLAIFSCLCLFMVISSTAQSDGPPVLDTEGRSLQRGVEYFIKPAITDVAGNLTLV 60
           M+L KPLAIFSCL LFM I STAQS  PPVLDT+GR LQRGVEYFIKPA+TDVAGNLTL+
Sbjct: 3   MLLQKPLAIFSCLFLFMAIYSTAQS--PPVLDTDGRPLQRGVEYFIKPAVTDVAGNLTLL 62

Query: 61  TRKGDPCPFNVGQVPLLSQDIGFAVTFTPYIEGEDTIREGRDLKVVFQAFTTCITSTEWR 120
           TRKGDPCPF+VGQVPL S DIG+ +    ++ GEDTIREGRDLKVVFQAF+ CITST WR
Sbjct: 63  TRKGDPCPFHVGQVPLRSTDIGYYILNLRFLAGEDTIREGRDLKVVFQAFSICITSTAWR 122

Query: 121 VGETEPTTGRRFVETGSDDTATGYFRIDNSD-GIYNIGWCPSDVPIRGRPRCGSAGILIE 180
           VGETEPTTGRR V TG+DDTATG+FRID SD G+YN+GWCPSDVPIRGRPRCG AGIL+E
Sbjct: 123 VGETEPTTGRRLVATGNDDTATGFFRIDKSDLGVYNLGWCPSDVPIRGRPRCGRAGILVE 182

Query: 181 KGVRLLALDGSAFPFEFVRA 200
           KGVRLLALDGSAFPFEFVRA
Sbjct: 183 KGVRLLALDGSAFPFEFVRA 200

[Close]

BLAST of Lag0006631 vs. NCBI nr
Match: XP_023521354.1 (alpha-amylase/subtilisin inhibitor-like [Cucurbita pepo subsp. pepo] >XP_023538197.1 alpha-amylase/subtilisin inhibitor-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 335.5 bits (859), Expect = 3.1e-88
Identity = 166/200 (83.00%), Postives = 176/200 (88.00%), Query Frame = 0


Query: 1   MMLHKPLAIFSCLCLFMVISSTAQSDGPPVLDTEGRSLQRGVEYFIKPAITDVAGNLTLV 60
           MML K L  FS LCL M +SS AQS  PPVLDTEGR LQ  VEYFIKPAITDVAGNLTL+
Sbjct: 1   MMLCKSLVAFSYLCLVMAVSSIAQS--PPVLDTEGRPLQINVEYFIKPAITDVAGNLTLL 60

Query: 61  TRKGDPCPFNVGQVPLLSQDIGFAVTFTPYIEGEDTIREGRDLKVVFQAFTTCITSTEWR 120
           TRKGD CPF+VGQVPL SQ+IGF VTFTPY+ GEDTIREGRDLKVVFQAF+ CITST WR
Sbjct: 61  TRKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWR 120

Query: 121 VGETEPTTGRRFVETGSDDTATGYFRIDNSD-GIYNIGWCPSDVPIRGRPRCGSAGILIE 180
           VGET+PTTGRRFVETG +DTATGYFRID  + GIYNIGWCPSDVPIRGRPRCG AGILIE
Sbjct: 121 VGETDPTTGRRFVETGDNDTATGYFRIDRDNFGIYNIGWCPSDVPIRGRPRCGRAGILIE 180

Query: 181 KGVRLLALDGSAFPFEFVRA 200
           KG+RLLALDGSAFPFEFVRA
Sbjct: 181 KGLRLLALDGSAFPFEFVRA 198

[Close]

BLAST of Lag0006631 vs. NCBI nr
Match: KAG7021121.1 (hypothetical protein SDJN02_17809, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 335.1 bits (858), Expect = 4.1e-88
Identity = 164/199 (82.41%), Postives = 175/199 (87.94%), Query Frame = 0


Query: 2   MLHKPLAIFSCLCLFMVISSTAQSDGPPVLDTEGRSLQRGVEYFIKPAITDVAGNLTLVT 61
           ML K L  FS LCL M +SS AQS  PPVLDTEGR LQ  VEYFIKPA+TDVAGNLTL+T
Sbjct: 1   MLCKSLVAFSYLCLVMAVSSIAQS--PPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLLT 60

Query: 62  RKGDPCPFNVGQVPLLSQDIGFAVTFTPYIEGEDTIREGRDLKVVFQAFTTCITSTEWRV 121
           RKGD CPF+VGQVPL SQ+IGF VTFTPY+ GEDTIREGRDLKVVFQAF+ CITST WRV
Sbjct: 61  RKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWRV 120

Query: 122 GETEPTTGRRFVETGSDDTATGYFRIDNSD-GIYNIGWCPSDVPIRGRPRCGSAGILIEK 181
           GET+PTTGRRFVETG DDTATGYFRID  + G+YNIGWCPSDVPIRGRPRCG AGILIEK
Sbjct: 121 GETDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIEK 180

Query: 182 GVRLLALDGSAFPFEFVRA 200
           G+RLLALDGSAFPFEFVRA
Sbjct: 181 GLRLLALDGSAFPFEFVRA 197

[Close]

BLAST of Lag0006631 vs. ExPASy Swiss-Prot
Match: G7LCV7 (Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 SV=1)

HSP 1 Score: 166.8 bits (421), Expect = 2.5e-40
Identity = 96/196 (48.98%), Postives = 125/196 (63.78%), Query Frame = 0


Query: 9   IFSCLCLFMVISSTAQSDGPPVLDTEGRSLQRGVEYFIKPAITDVAGNLTLVTRKGDPCP 68
           I + + L +V +S AQ     V+DT G  ++   +YFI+PAIT   G+LTLVTR  + CP
Sbjct: 10  ILANVWLLVVTTSIAQF----VIDTSGEPVENDEDYFIRPAITGNGGSLTLVTR--NSCP 69

Query: 69  FNVGQVPLLSQDIGFAVTFTPYIEG--EDTIREGRDLKVVFQAFTTCITSTEWRVGETEP 128
           FNVG  P   Q  GFAV  +P++    ED +R GRDL+V+FQA T+C  STEWR+GE + 
Sbjct: 70  FNVGLDPDAPQ--GFAVLLSPFVSNREEDEVRLGRDLRVIFQAGTSCGQSTEWRLGERDA 129

Query: 129 TTGRRFVETGSDDTATG----YFRI--DNSDGIYNIGWCPSDVPIRGRPRCGSAGILIEK 188
           TTGRRF+ TG DD+  G    +FRI    S GI+NI WCP++V    +  CG+ GI+ E 
Sbjct: 130 TTGRRFIITGRDDSTVGSYGNFFRIVQTPSRGIFNIQWCPTEVCPSCKFECGTVGIVREN 189

Query: 189 GVRLLALDGSAFPFEF 197
           G  LLALDGSA P  F
Sbjct: 190 GKILLALDGSALPVAF 197

[Close]

BLAST of Lag0006631 vs. ExPASy Swiss-Prot
Match: G7LCV1 (Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 SV=1)

HSP 1 Score: 142.5 bits (358), Expect = 5.1e-33
Identity = 80/200 (40.00%), Postives = 116/200 (58.00%), Query Frame = 0


Query: 5   KPLAIFSCLCLFMVISSTAQSDGPPVLDTEGRSLQRGVEYFIKPAITDVAGNLTLVTRKG 64
           + L I + +CLF+  ++ AQ     VLDT G  ++   EYFI+P IT+  G  T+V+R  
Sbjct: 8   RTLIILAHVCLFITTTTIAQF----VLDTVGEPVEGDEEYFIRPVITNKGGRSTMVSR-N 67

Query: 65  DPCPFNVGQVPLLSQDIGFAVTFTPYIEGE--DTIREGRDLKVVFQAFTTCITSTEWRVG 124
           + CP +VG + L     G  V FTP+      D +R  RDL++ FQA ++C+ STEWR+G
Sbjct: 68  ESCPLHVG-LELTGLGRGLVVKFTPFAPHHDFDDVRVNRDLRITFQASSSCVQSTEWRLG 127

Query: 125 ETEPTTGRRFVETGSDDTATG----YFRIDNS--DGIYNIGWCPSDVPIRGRPRCGSAGI 184
           E +  +GRR + TG+D    G    +FRI  +  +G+YNI WCP++V    +  CG+  +
Sbjct: 128 EKDTKSGRRLIITGTDSATNGSYGNFFRIVETPLEGMYNIQWCPTEVCPSCKFECGTVDM 187

Query: 185 LIEKGVRLLALDGSAFPFEF 197
           L E G  LLALDG   P  F
Sbjct: 188 LNENGKILLALDGGPLPLVF 201

[Close]

BLAST of Lag0006631 vs. ExPASy Swiss-Prot
Match: A0A072TH68 (Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 1.1e-32
Identity = 82/202 (40.59%), Postives = 117/202 (57.92%), Query Frame = 0


Query: 2   MLHKPLAIFSCLCLFMVISSTAQSDGPPVLDTEGRSLQRGVEYFIKPAITDVAGNLTLVT 61
           M  + L IF    +++++++T  S    V+DT G  ++   EYFI+PAIT   G  TLVT
Sbjct: 1   MSTRSLTIFILAHVWLLMATT--SIAQFVIDTSGEPVEDDEEYFIRPAITGNGGGSTLVT 60

Query: 62  RKGDPCPFNVGQVPLLSQDIGFAVTFTPYI--EGEDTIREGRDLKVVFQAFTTCITSTEW 121
             G PCP +VG +      +G AV FTP+     +D +R  RDL+V F   T+C  ST+W
Sbjct: 61  GNG-PCPLHVG-LDNTEGTLGVAVKFTPFAPQHDDDDVRLNRDLRVTFLTSTSCGQSTDW 120

Query: 122 RVGETEPTTGRRFVETGSDDTA---TGYFRI--DNSDGIYNIGWCPSDVPIRGRPRCGSA 181
           R+GE + T+GRR + TG D+ A     +FRI    + G YNI WCP++     + +CG+ 
Sbjct: 121 RLGEKDATSGRRLIVTGRDNGAGSQGNFFRIVQTQTGGTYNIQWCPTEACPSCKVQCGTV 180

Query: 182 GILIEKGVRLLALDGSAFPFEF 197
           G++ E G  LLALDG A P  F
Sbjct: 181 GVIRENGKNLLALDGDALPVVF 198

[Close]

BLAST of Lag0006631 vs. ExPASy Swiss-Prot
Match: P13087 (Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3)

HSP 1 Score: 94.0 bits (232), Expect = 2.1e-18
Identity = 67/190 (35.26%), Postives = 92/190 (48.42%), Query Frame = 0


Query: 21  STAQSDGPPVLDTEGRSLQRGVEYFIKPAITDVAGNLTL--VTRKGD-PCPFNVGQVPLL 80
           S A S   PVLD +G  L+ G  Y+I P + D  G LT+   T  G   CP  V Q    
Sbjct: 27  SAADSAPNPVLDIDGEKLRTGTNYYIVPVLRDHGGGLTVSATTPNGTFVCPPRVVQT-RK 86

Query: 81  SQDIGFAVTFTPYIEGEDTIREGRDLKVVFQAFTTC--ITSTEWRVGETEPTTGRRFVET 140
             D    + F P    ED +R   DL + F AF  C   +ST WR+ + + +TG+ FV  
Sbjct: 87  EVDHDRPLAFFPENPKEDVVRVSTDLNINFSAFMPCRWTSSTVWRLDKYDESTGQYFVTI 146

Query: 141 G------SDDTATGYFRIDN--SDGIYNIGWCPSDVPIRGRPRCGSAGILI-EKGVRLLA 197
           G        +T + +F+I+     G Y + +CP+ V    + +CG  GI I +KG R LA
Sbjct: 147 GGVKGNPGPETISSWFKIEEFCGSGFYKLVFCPT-VCGSCKVKCGDVGIYIDQKGRRRLA 206

[Close]

BLAST of Lag0006631 vs. ExPASy Swiss-Prot
Match: P29421 (Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica OX=39947 GN=RASI PE=1 SV=2)

HSP 1 Score: 87.4 bits (215), Expect = 2.0e-16
Identity = 64/180 (35.56%), Postives = 82/180 (45.56%), Query Frame = 0


Query: 3   LHKPLAIFSCLCLFMVISSTAQSDGPPVLDTEGRSLQRGVEYFIKPAITDVAGNLTLVTR 62
           L  PL + S L     IS +  +  PPV DTEG  L     Y++ PA     G LT+  R
Sbjct: 4   LRLPLILLSLL----AISFSCSAAPPPVYDTEGHELSADGSYYVLPASPGHGGGLTMAPR 63

Query: 63  KGDPCPFNVGQVPLLSQDIGFAVTFTPY----IEGEDTIREGRDLKVVFQAFTTCITSTE 122
              PCP  V Q     +  GF V FTP+       + TIR   D+++ F A T C+ STE
Sbjct: 64  V-LPCPLLVAQ-ETDERRKGFPVRFTPWGGAAAPEDRTIRVSTDVRIRFNAATICVQSTE 123

Query: 123 WRVGETEPTTGRRFVETG-----SDDTATGYFRIDNSDGIYNIGWCPSDVPIRGRPRCGS 174
           W VG+ EP TG R V TG     S       FR++   G Y +  C       G  R G+
Sbjct: 124 WHVGD-EPLTGARRVVTGPLIGPSPSGRENAFRVEKYGGGYKLVSCRDSCQDLGVSRDGA 176

[Close]

BLAST of Lag0006631 vs. ExPASy TrEMBL
Match: A0A6J1FD70 (alpha-amylase/subtilisin inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC111444311 PE=3 SV=1)

HSP 1 Score: 336.7 bits (862), Expect = 6.8e-89
Identity = 165/200 (82.50%), Postives = 176/200 (88.00%), Query Frame = 0


Query: 1   MMLHKPLAIFSCLCLFMVISSTAQSDGPPVLDTEGRSLQRGVEYFIKPAITDVAGNLTLV 60
           MML K L  FS LCL M +SS AQS  PPVLDTEGR LQ  VEYFIKPA+TDVAGNLTL+
Sbjct: 1   MMLCKSLVAFSYLCLVMAVSSIAQS--PPVLDTEGRPLQINVEYFIKPAVTDVAGNLTLL 60

Query: 61  TRKGDPCPFNVGQVPLLSQDIGFAVTFTPYIEGEDTIREGRDLKVVFQAFTTCITSTEWR 120
           TRKGD CPF+VGQVPL SQ+IGF VTFTPY+ GEDTIREGRDLKVVFQAF+ CITST WR
Sbjct: 61  TRKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPGEDTIREGRDLKVVFQAFSICITSTAWR 120

Query: 121 VGETEPTTGRRFVETGSDDTATGYFRIDNSD-GIYNIGWCPSDVPIRGRPRCGSAGILIE 180
           VGET+PTTGRRFVETG DDTATGYFRID  + G+YNIGWCPSDVPIRGRPRCG AGILIE
Sbjct: 121 VGETDPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIE 180

Query: 181 KGVRLLALDGSAFPFEFVRA 200
           KG+RLLALDGSAFPFEFVRA
Sbjct: 181 KGLRLLALDGSAFPFEFVRA 198

[Close]

BLAST of Lag0006631 vs. ExPASy TrEMBL
Match: A0A6J1DBS1 (miraculin-like OS=Momordica charantia OX=3673 GN=LOC111018865 PE=3 SV=1)

HSP 1 Score: 336.3 bits (861), Expect = 8.9e-89
Identity = 163/200 (81.50%), Postives = 178/200 (89.00%), Query Frame = 0


Query: 1   MMLHKPLAIFSCLCLFMVISSTAQSDGPPVLDTEGRSLQRGVEYFIKPAITDVAGNLTLV 60
           M+L KPLAIFSCL LFM I STAQS  PPVLDT+GR LQRGVEYFIKPA+TDVAGNLTL+
Sbjct: 3   MLLQKPLAIFSCLFLFMAIYSTAQS--PPVLDTDGRPLQRGVEYFIKPAVTDVAGNLTLL 62

Query: 61  TRKGDPCPFNVGQVPLLSQDIGFAVTFTPYIEGEDTIREGRDLKVVFQAFTTCITSTEWR 120
           TRKGDPCPF+VGQVPL S DIG+ +    ++ GEDTIREGRDLKVVFQAF+ CITST WR
Sbjct: 63  TRKGDPCPFHVGQVPLRSTDIGYYILNLRFLAGEDTIREGRDLKVVFQAFSICITSTAWR 122

Query: 121 VGETEPTTGRRFVETGSDDTATGYFRIDNSD-GIYNIGWCPSDVPIRGRPRCGSAGILIE 180
           VGETEPTTGRR V TG+DDTATG+FRID SD G+YN+GWCPSDVPIRGRPRCG AGIL+E
Sbjct: 123 VGETEPTTGRRLVATGNDDTATGFFRIDKSDLGVYNLGWCPSDVPIRGRPRCGRAGILVE 182

Query: 181 KGVRLLALDGSAFPFEFVRA 200
           KGVRLLALDGSAFPFEFVRA
Sbjct: 183 KGVRLLALDGSAFPFEFVRA 200

[Close]

BLAST of Lag0006631 vs. ExPASy TrEMBL
Match: A0A0A0LGB4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021000 PE=3 SV=1)

HSP 1 Score: 332.0 bits (850), Expect = 1.7e-87
Identity = 159/200 (79.50%), Postives = 174/200 (87.00%), Query Frame = 0


Query: 1   MMLHKPL-AIFSCLCLFMVISSTAQSDGPPVLDTEGRSLQRGVEYFIKPAITDVAGNLTL 60
           M  HK L A+FS LC FM I STAQ DGPPVLDTEGR LQR VEYFIKPAITDVAGNLTL
Sbjct: 2   MRFHKSLIALFSWLCFFMAIPSTAQFDGPPVLDTEGRPLQRDVEYFIKPAITDVAGNLTL 61

Query: 61  VTRKGDPCPFNVGQVPLLSQDIGFAVTFTPYIEGEDTIREGRDLKVVFQAFTTCITSTEW 120
           +TRKGD CPF VGQVPLLSQ+IGF VT TPY+EG+DTIREGRDLK VFQAFT CIT T+W
Sbjct: 62  ITRKGDQCPFYVGQVPLLSQEIGFGVTLTPYLEGDDTIREGRDLKFVFQAFTICITGTQW 121

Query: 121 RVGETEPTTGRRFVETGSDDTATGYFRIDNSD-GIYNIGWCPSDVPIRGRPRCGSAGILI 180
           +VGE +P +GRRFV+ G+D+TATGYFRID S+ G+YNIGWCPSDVP +GRPRCGSAGILI
Sbjct: 122 KVGEEDPKSGRRFVKLGNDNTATGYFRIDKSNLGVYNIGWCPSDVPFKGRPRCGSAGILI 181

Query: 181 EKGVRLLALDGSAFPFEFVR 199
           EKGVR LALDG AFPFEFVR
Sbjct: 182 EKGVRFLALDGPAFPFEFVR 201

[Close]

BLAST of Lag0006631 vs. ExPASy TrEMBL
Match: A0A6J1HLP6 (kunitz trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111465676 PE=3 SV=1)

HSP 1 Score: 325.5 bits (833), Expect = 1.6e-85
Identity = 161/200 (80.50%), Postives = 171/200 (85.50%), Query Frame = 0


Query: 1   MMLHKPLAIFSCLCLFMVISSTAQSDGPPVLDTEGRSLQRGVEYFIKPAITDVAGNLTLV 60
           MML K L  FS LCL M  SS AQS  PPVLD EGR L+  VEYFIKPAITDVAGNLTL+
Sbjct: 1   MMLCKSLVAFSYLCLVMAASSIAQS--PPVLDIEGRPLEINVEYFIKPAITDVAGNLTLL 60

Query: 61  TRKGDPCPFNVGQVPLLSQDIGFAVTFTPYIEGEDTIREGRDLKVVFQAFTTCITSTEWR 120
           TRKGD CPF+VGQVPL SQ+IGF VTFTPY+  ED IREGRDLKVVFQAF+ CITST WR
Sbjct: 61  TRKGDQCPFHVGQVPLRSQEIGFGVTFTPYLPSEDMIREGRDLKVVFQAFSICITSTAWR 120

Query: 121 VGETEPTTGRRFVETGSDDTATGYFRIDNSD-GIYNIGWCPSDVPIRGRPRCGSAGILIE 180
           VGE +PTTGRRFVETG DDTATGYFRID  + G+YNIGWCPSDVPIRGRPRCG AGILIE
Sbjct: 121 VGEADPTTGRRFVETGDDDTATGYFRIDRDNFGVYNIGWCPSDVPIRGRPRCGRAGILIE 180

Query: 181 KGVRLLALDGSAFPFEFVRA 200
           KG+RLLALDGSAFPFEFVRA
Sbjct: 181 KGLRLLALDGSAFPFEFVRA 198

[Close]

BLAST of Lag0006631 vs. ExPASy TrEMBL
Match: A0A1S3C0M7 (endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo OX=3656 GN=LOC103495287 PE=3 SV=1)

HSP 1 Score: 323.9 bits (829), Expect = 4.6e-85
Identity = 159/201 (79.10%), Postives = 170/201 (84.58%), Query Frame = 0


Query: 1   MMLHKPL-AIFSCLCLFMVISSTA-QSDGPPVLDTEGRSLQRGVEYFIKPAITDVAGNLT 60
           M  HK L +IFS LC FM I STA Q DGPPVLDTEGR LQR  EYFIKPAITDVAGNLT
Sbjct: 2   MRFHKSLFSIFSLLCFFMAIPSTAGQFDGPPVLDTEGRPLQRDAEYFIKPAITDVAGNLT 61

Query: 61  LVTRKGDPCPFNVGQVPLLSQDIGFAVTFTPYIEGEDTIREGRDLKVVFQAFTTCITSTE 120
           L+TRKGD CPF VGQVPLLSQ+ GFAV+  PY EGEDTIREGRDLK VFQ FT CIT T+
Sbjct: 62  LITRKGDQCPFYVGQVPLLSQETGFAVSLAPYREGEDTIREGRDLKFVFQVFTICITGTQ 121

Query: 121 WRVGETEPTTGRRFVETGSDDTATGYFRIDNSD-GIYNIGWCPSDVPIRGRPRCGSAGIL 180
           W+VGE +P TGRRFV+ G D+TATGYFRID SD G+YNIGWCPSDVPI+GRPRCGSAGIL
Sbjct: 122 WKVGEADPKTGRRFVKLGYDNTATGYFRIDKSDLGVYNIGWCPSDVPIKGRPRCGSAGIL 181

Query: 181 IEKGVRLLALDGSAFPFEFVR 199
           IEKGVR LALDG AFPFEFVR
Sbjct: 182 IEKGVRFLALDGPAFPFEFVR 202

[Close]

BLAST of Lag0006631 vs. TAIR 10
Match: AT1G73260.1 (kunitz trypsin inhibitor 1 )

HSP 1 Score: 82.4 bits (202), Expect = 4.5e-16
Identity = 63/196 (32.14%), Postives = 90/196 (45.92%), Query Frame = 0


Query: 13  LCLFMVISSTAQSDGPPVLDTEGRSLQRGVEYFIKPAITDVAGNLTLVTRKGDPCPFNVG 72
           L L  V++S A      V+D +G ++     Y++ P I    G LTL  R G PCP+++ 
Sbjct: 16  LALTAVLASNAYG---AVVDIDGNAMFH-ESYYVLPVIRGRGGGLTLAGRGGQPCPYDIV 75

Query: 73  QVPLLSQDIGFAVTFTPYIEGEDTIREGRDLKVVFQ-AFTTCITSTEWRVGETEPTTGRR 132
           Q      D G  V F+ +      + E ++L +      T CI ST WRVGE +    + 
Sbjct: 76  Q-ESSEVDEGIPVKFSNWRLKVAFVPESQNLNIETDVGATICIQSTYWRVGEFDHERKQY 135

Query: 133 FVETG------SDDTATGYFRIDNS-DGIYNIGWCPSDVPIRGRPRCGSAGILI-EKGVR 192
           FV  G        D+   +F+I+ S +  Y   +CP      G P+C   GI I E GVR
Sbjct: 136 FVVAGPKPEGFGQDSLKSFFKIEKSGEDAYKFVFCPRTCD-SGNPKCSDVGIFIDELGVR 195

Query: 193 LLALDGSAFPFEFVRA 200
            LAL    F   F +A
Sbjct: 196 RLALSDKPFLVMFKKA 205

[Close]

BLAST of Lag0006631 vs. TAIR 10
Match: AT1G17860.1 (Kunitz family trypsin and protease inhibitor protein )

HSP 1 Score: 74.7 bits (182), Expect = 9.3e-14
Identity = 61/201 (30.35%), Postives = 90/201 (44.78%), Query Frame = 0


Query: 7   LAIFSCLCLFMVISS-TAQSDGPPVLDTEGRSLQRGVEYFIKPAITDVAGNLTLVTRKGD 66
           L IF  L +F+     T ++   PV D  G+SL  GV Y+I P I    G LT+   K +
Sbjct: 5   LYIFLLLAVFISHRGVTTEAAVEPVKDINGKSLLTGVNYYILPVIRGRGGGLTMSNLKTE 64

Query: 67  PCPFNVGQVPL-LSQDIGFAVTFTPYIEGEDTIREGRDLKVVFQAFTTCITSTEWRVGET 126
            CP +V Q    +SQ  G  V F+PY +   TI    D+ + F        ++ W +   
Sbjct: 65  TCPTSVIQDQFEVSQ--GLPVKFSPY-DKSRTIPVSTDVNIKFS------PTSIWELANF 124

Query: 127 EPTTGRRFVET------GSDDTATGYFRIDNSDGIYNIGWCPSDVPIRGRPRCGSAGILI 186
           + TT + F+ T          T   +F+ID  +  Y I +CP+ V    +  C   G+ +
Sbjct: 125 DETTKQWFISTCGVEGNPGQKTVDNWFKIDKFEKDYKIRFCPT-VCNFCKVICRDVGVFV 184

Query: 187 EKGVRLLALDGSAFPFEFVRA 200
           + G R LAL        F RA
Sbjct: 185 QDGKRRLALSDVPLKVMFKRA 195

The following BLAST results are available for this feature:

BLAST of Lag0006631 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038888505.1	1.3e-94	85.93	kunitz type trypsin inhibitor 111-like [Benincasa hispida]	[more]
XP_022938134.1	1.4e-88	82.50	alpha-amylase/subtilisin inhibitor-like [Cucurbita moschata]	[more]
XP_022150807.1	1.8e-88	81.50	miraculin-like [Momordica charantia]	[more]
XP_023521354.1	3.1e-88	83.00	alpha-amylase/subtilisin inhibitor-like [Cucurbita pepo subsp. pepo] >XP_0235381...	[more]
KAG7021121.1	4.1e-88	82.41	hypothetical protein SDJN02_17809, partial [Cucurbita argyrosperma subsp. argyro...	[more]

BLAST of Lag0006631 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
G7LCV7	2.5e-40	48.98	Kunitz type trypsin inhibitor 111 OS=Medicago truncatula OX=3880 GN=KPI111 PE=1 ...	[more]
G7LCV1	5.1e-33	40.00	Kunitz type trypsin inhibitor 106 OS=Medicago truncatula OX=3880 GN=KPI106 PE=1 ...	[more]
A0A072TH68	1.1e-32	40.59	Kunitz type trypsin inhibitor 104 OS=Medicago truncatula OX=3880 GN=KPI104 PE=1 ...	[more]
P13087	2.1e-18	35.26	Miraculin OS=Synsepalum dulcificum OX=3743 PE=1 SV=3	[more]
P29421	2.0e-16	35.56	Alpha-amylase/subtilisin inhibitor OS=Oryza sativa subsp. japonica OX=39947 GN=R...	[more]

BLAST of Lag0006631 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FD70	6.8e-89	82.50	alpha-amylase/subtilisin inhibitor-like OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1DBS1	8.9e-89	81.50	miraculin-like OS=Momordica charantia OX=3673 GN=LOC111018865 PE=3 SV=1	[more]
A0A0A0LGB4	1.7e-87	79.50	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G021000 PE=3 SV=1	[more]
A0A6J1HLP6	1.6e-85	80.50	kunitz trypsin inhibitor 1-like OS=Cucurbita maxima OX=3661 GN=LOC111465676 PE=3...	[more]
A0A1S3C0M7	4.6e-85	79.10	endogenous alpha-amylase/subtilisin inhibitor-like OS=Cucumis melo OX=3656 GN=LO...	[more]

BLAST of Lag0006631 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G73260.1	4.5e-16	32.14	kunitz trypsin inhibitor 1	[more]
AT1G17860.1	9.3e-14	30.35	Kunitz family trypsin and protease inhibitor protein	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002160	Proteinase inhibitor I3, Kunitz legume	SMART	SM00452	kul_2	coord: 29..199 e-value: 1.0E-25 score: 101.4
IPR002160	Proteinase inhibitor I3, Kunitz legume	PFAM	PF00197	Kunitz_legume	coord: 30..197 e-value: 2.4E-37 score: 128.5
IPR002160	Proteinase inhibitor I3, Kunitz legume	PANTHER	PTHR33107	KUNITZ TRYPSIN INHIBITOR 2	coord: 8..199
IPR002160	Proteinase inhibitor I3, Kunitz legume	PROSITE	PS00283	SOYBEAN_KUNITZ	coord: 30..46
IPR002160	Proteinase inhibitor I3, Kunitz legume	CDD	cd00178	STI	coord: 29..198 e-value: 1.49732E-35 score: 120.91
None	No IPR available	GENE3D	2.80.10.50		coord: 27..199 e-value: 3.1E-40 score: 139.6
None	No IPR available	PANTHER	PTHR33107:SF51	PUTATIVE-RELATED	coord: 8..199
IPR011065	Kunitz inhibitor STI-like superfamily	SUPERFAMILY	50386	STI-like	coord: 28..198

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0006631.1	Lag0006631.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010951	negative regulation of endopeptidase activity
molecular_function	GO:0004866	endopeptidase inhibitor activity