Lag0006626 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0006626
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionincreased DNA methylation 2-like
Locationchr6: 44258402 .. 44261159 (+)
RNA-Seq ExpressionLag0006626
SyntenyLag0006626
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATGGCGGCTTTCCCCAGGGTTCAAAATCTCACATAGCTGTTATGCCAACTAAAACACCAGACGGTGATCAACATTTTCTCTTGTACTTCATCGCTGGTATCTACTTTGGGCCTGACCTCAAAGGTGAACGGCCACTGAAGTCAGCTCTGCAGAGGCTTGCTGAGGGATTGCCTCCCTATACTTCTGATCAACTTGCTGGATCTCAAATGAAGATGGTGGAAGTAGAGCGCATTTTTTATTATGTCTTGAGGAAGGCTGATGAATCTCTTGTTATGAAAATGTCTTTATTGCATCAGTTCTTCCATGGCAACTTTCCTGCCCAAGGACGAGATATTAGTTTTCCTCAGTTTCCTGATCTCTTTCCACCCGAATTCCATCCCCATTCTCGGTCGAAGAACTGGTATAGGTTTATCGAAAACCTTTCATTCATCCACTACCCAGAAGTCTACTACCTCAATTCTGAGGATGTTGAAAGATTTAAGAGGCTAACAGGACTGGATGACTTCCTTTTGGATAGAGATGCAGCGAGGTCGCACAATTTTTCAGTTCGCAAAGTTTCACCCAATGTTGAAGCTACAGATAATTCATCAAATAAACAGTTTTCTCCTCTTAAAGATGATCTGCAATATGACTCTGTTACTGCACCTGTTCGTAGTGTATCATATAATGGTAATTTGACACCTCCACATTCCAAATCTGACTCTGATCTTGGGGAGAAGAAATTTGGTCCAGCAATGCTTTTTCTTCCCAGACAACCATGTGAAGAAGATTGGGCGAATCTAGTAGCTGCTACAGATTCAGGATTTGCATTGACTGGAACTGCAGCAATGGGGCACGTTGGACCAATAATTGGATTAATGGACATTGGGGAATGTGAAGACTCGTACTTGTTTCGTGTGTCTCTTCCTGGCGTTAAAAGAGATGGAAGTAAGTTTCTTTCATTAAAGTATTTCAATGCTCTGAATCTCACATGATTGCTGCAACTGCACATGATTATCCTATTAGAGCATCCCAAGGGTTGGGTAGAACAAATTTTTTGAATTGATAGGGAAAAAAAGAGCCTCCATTATTACCCATTCAAAAATCCTAGGGACTATTTGAGCATATATTTTAGAAGTTGGTTTCAAAAGTTGATTCTTAGAGTAATTTTTCAAAAACTGTTTTCACTAATTTAATTTAAAAGACTGTTTGAAAAGACATTTTCTTAGTTTAAAAATATAAGACACTTAAACCAGCCTATACATAAAAACGAAATGATTAATGATTGTGAATGATCAATTATTTTAGCGTAGTTAAAAAGAGGTGAGGAATTCAAACTTCTAACCTCTTGGTCGAAGGTACATGTCGATTACCGTTGGAAACCATAACATTCTTTTTGTTTTCTTTTATCTTAATTGTTTTATAGAACTGTCCTTTCAAACAGTTTACTGAGTTAGAAAACAATTTTATATTTTGAAAATTATAAAGTAGCTTTTAAACAGTTTTCACACAGTCCCTTCACCCATTTTCTTTAATTTAATAATTCCACAAGTAACAAATACCCAAATCATCTAACCCATCATACACCCCACATGTCTCTCTTCCTATTAATTAAGAAGAGGGAGGAGGTGGGAGAGGGTAAGATTAGTTTAGTTATATATATACATTTTTGGAGAAAAGGGTAAGATTAGTTTTCACATCTTAGGAGGAAAAAAAAAAAAAGATCTTGGTGACAATCAAGGAAATGGATATATTAACTCTTTCTTCCTTTCTACATATGGTTCTACTTAAGAATTTTTTACTTTCTTCCCAGAGGATCACAACTTCCTAGGTCATAAAGGATCTTAGAAACAACATTGTACATATATTATAACCTCGATGATATTGGTTAAATCACTCCATTGCTTCAATTATGTTTGGATAATTATTTAGTTAAGTTATAAATTTCTTCCTTGAACTTTGATAATCATGTCCATTCAACTAGAAACTCTGACATGCCTATTAGATACATTTCATGTGATTTGACATGATAAATGATAGGAACATCTGTTCTTTCTTGGTTGATGTATGGGATGAATATCACTTTAGTTTGTTTATGATAGGAATGTACCTAAGGGTAATTAGGAATATTAGTAAAGATATATTAGCAGTTAGTTAGGGAGTTGTTAGAGTATGGAGTTATAAATAGAGAAAGTGGGAAAAGGGGAAGGGAGGAATTGAGTAGTGAGTGATTACTCTTGGGTGTTCTCAAGAGAGGGAGGGTCCAAGTACATCGAACACTTGGGATATTGTAATAATACGTCATAGTTCTATTCCGGTTCTTATCAGTTTAGCTGTCAACTTGTTTCTTCCATCTATCTACCATATCACGTCAAACCAGGCAACATTTATTTATTAGATTAAGGGATGTAACTGTTTACATTTAAAGGCCAAATAGAAAACTATCAAAATTAACTGAATTTTATAACTTTGGCCATTTTTTCAGTGCTTTCCTGTCATAATTTGATGAGACTTTTGATTCATTGATCAGGGGGATTTAACTGTGAAGTTGAAAAGGATGGCAGAGTGGTGATACAGGGAGTTACAACAACAGGTGAGAGAACAGTGAAAAAACATTCTCAAGTGTTTGAAATGCTAACCCACAACCTGTGCCCACCAGGAGAGTTTTCACTCTCATTTCAGCTACCTGGCCCAGTTGATCCTCAACAATTCTTTGCTAACTTTGGCATTGATGGGATTCTTGAAGGAGTTGTAATGAAAGATTTACAGTCATGA

mRNA sequence

ATGGATGGCGGCTTTCCCCAGGGTTCAAAATCTCACATAGCTGTTATGCCAACTAAAACACCAGACGGTGATCAACATTTTCTCTTGTACTTCATCGCTGGTATCTACTTTGGGCCTGACCTCAAAGGTGAACGGCCACTGAAGTCAGCTCTGCAGAGGCTTGCTGAGGGATTGCCTCCCTATACTTCTGATCAACTTGCTGGATCTCAAATGAAGATGGTGGAAGTAGAGCGCATTTTTTATTATGTCTTGAGGAAGGCTGATGAATCTCTTGTTATGAAAATGTCTTTATTGCATCAGTTCTTCCATGGCAACTTTCCTGCCCAAGGACGAGATATTAGTTTTCCTCAGTTTCCTGATCTCTTTCCACCCGAATTCCATCCCCATTCTCGGTCGAAGAACTGGTATAGGTTTATCGAAAACCTTTCATTCATCCACTACCCAGAAGTCTACTACCTCAATTCTGAGGATGTTGAAAGATTTAAGAGGCTAACAGGACTGGATGACTTCCTTTTGGATAGAGATGCAGCGAGGTCGCACAATTTTTCAGTTCGCAAAGTTTCACCCAATGTTGAAGCTACAGATAATTCATCAAATAAACAGTTTTCTCCTCTTAAAGATGATCTGCAATATGACTCTGTTACTGCACCTGTTCGTAGTGTATCATATAATGGTAATTTGACACCTCCACATTCCAAATCTGACTCTGATCTTGGGGAGAAGAAATTTGGTCCAGCAATGCTTTTTCTTCCCAGACAACCATGTGAAGAAGATTGGGCGAATCTAGTAGCTGCTACAGATTCAGGATTTGCATTGACTGGAACTGCAGCAATGGGGCACGTTGGACCAATAATTGGATTAATGGACATTGGGGAATGTGAAGACTCGTACTTGTTTCGTGTGTCTCTTCCTGGCGTTAAAAGAGATGGAAGGGGATTTAACTGTGAAGTTGAAAAGGATGGCAGAGTGGTGATACAGGGAGTTACAACAACAGGTGAGAGAACAGTGAAAAAACATTCTCAAGTGTTTGAAATGCTAACCCACAACCTGTGCCCACCAGGAGAGTTTTCACTCTCATTTCAGCTACCTGGCCCAGTTGATCCTCAACAATTCTTTGCTAACTTTGGCATTGATGGGATTCTTGAAGGAGTTGTAATGAAAGATTTACAGTCATGA

Coding sequence (CDS)

ATGGATGGCGGCTTTCCCCAGGGTTCAAAATCTCACATAGCTGTTATGCCAACTAAAACACCAGACGGTGATCAACATTTTCTCTTGTACTTCATCGCTGGTATCTACTTTGGGCCTGACCTCAAAGGTGAACGGCCACTGAAGTCAGCTCTGCAGAGGCTTGCTGAGGGATTGCCTCCCTATACTTCTGATCAACTTGCTGGATCTCAAATGAAGATGGTGGAAGTAGAGCGCATTTTTTATTATGTCTTGAGGAAGGCTGATGAATCTCTTGTTATGAAAATGTCTTTATTGCATCAGTTCTTCCATGGCAACTTTCCTGCCCAAGGACGAGATATTAGTTTTCCTCAGTTTCCTGATCTCTTTCCACCCGAATTCCATCCCCATTCTCGGTCGAAGAACTGGTATAGGTTTATCGAAAACCTTTCATTCATCCACTACCCAGAAGTCTACTACCTCAATTCTGAGGATGTTGAAAGATTTAAGAGGCTAACAGGACTGGATGACTTCCTTTTGGATAGAGATGCAGCGAGGTCGCACAATTTTTCAGTTCGCAAAGTTTCACCCAATGTTGAAGCTACAGATAATTCATCAAATAAACAGTTTTCTCCTCTTAAAGATGATCTGCAATATGACTCTGTTACTGCACCTGTTCGTAGTGTATCATATAATGGTAATTTGACACCTCCACATTCCAAATCTGACTCTGATCTTGGGGAGAAGAAATTTGGTCCAGCAATGCTTTTTCTTCCCAGACAACCATGTGAAGAAGATTGGGCGAATCTAGTAGCTGCTACAGATTCAGGATTTGCATTGACTGGAACTGCAGCAATGGGGCACGTTGGACCAATAATTGGATTAATGGACATTGGGGAATGTGAAGACTCGTACTTGTTTCGTGTGTCTCTTCCTGGCGTTAAAAGAGATGGAAGGGGATTTAACTGTGAAGTTGAAAAGGATGGCAGAGTGGTGATACAGGGAGTTACAACAACAGGTGAGAGAACAGTGAAAAAACATTCTCAAGTGTTTGAAATGCTAACCCACAACCTGTGCCCACCAGGAGAGTTTTCACTCTCATTTCAGCTACCTGGCCCAGTTGATCCTCAACAATTCTTTGCTAACTTTGGCATTGATGGGATTCTTGAAGGAGTTGTAATGAAAGATTTACAGTCATGA

Protein sequence

MDGGFPQGSKSHIAVMPTKTPDGDQHFLLYFIAGIYFGPDLKGERPLKSALQRLAEGLPPYTSDQLAGSQMKMVEVERIFYYVLRKADESLVMKMSLLHQFFHGNFPAQGRDISFPQFPDLFPPEFHPHSRSKNWYRFIENLSFIHYPEVYYLNSEDVERFKRLTGLDDFLLDRDAARSHNFSVRKVSPNVEATDNSSNKQFSPLKDDLQYDSVTAPVRSVSYNGNLTPPHSKSDSDLGEKKFGPAMLFLPRQPCEEDWANLVAATDSGFALTGTAAMGHVGPIIGLMDIGECEDSYLFRVSLPGVKRDGRGFNCEVEKDGRVVIQGVTTTGERTVKKHSQVFEMLTHNLCPPGEFSLSFQLPGPVDPQQFFANFGIDGILEGVVMKDLQS
Homology
BLAST of Lag0006626 vs. NCBI nr
Match: XP_022938125.1 (increased DNA methylation 2-like [Cucurbita moschata] >XP_022938126.1 increased DNA methylation 2-like [Cucurbita moschata])

HSP 1 Score: 703.0 bits (1813), Expect = 1.5e-198
Identity = 341/392 (86.99%), Postives = 360/392 (91.84%), Query Frame = 0

Query: 1   MDGGFPQGSKSHIAVMPTKTPDGDQHFLLYFIAGIYFGPDLKGERPLKSALQRLAEGLPP 60
           MDG FPQ SKS + VMPTKTPDGDQHFLLY IAGIYFGPDLKGERP+KS LQRLA  LPP
Sbjct: 1   MDGSFPQDSKSPLTVMPTKTPDGDQHFLLYLIAGIYFGPDLKGERPMKSVLQRLAAALPP 60

Query: 61  YTSDQLAGSQMKMVEVERIFYYVLRKADESLVMKMSLLHQFFHGNFPAQGRDISFPQFPD 120
           YTSDQLAGSQ+K+VEVERIFYYVLRKADESL+MK SLLHQFF G FPAQGRDISFPQFPD
Sbjct: 61  YTSDQLAGSQIKVVEVERIFYYVLRKADESLIMKTSLLHQFFQGKFPAQGRDISFPQFPD 120

Query: 121 LFPPEFHPHSRSKNWYRFIENLSFIHYPEVYYLNSEDVERFKRLTGLDDFLLDRDAARSH 180
           LFPPE HPHSRSKNWYRFIENLSFIH P+V YLNSEDVERFKRLTGL+DFLLDRDAARSH
Sbjct: 121 LFPPELHPHSRSKNWYRFIENLSFIHNPDVSYLNSEDVERFKRLTGLNDFLLDRDAARSH 180

Query: 181 NFSVRKVSPNVEATD--NSSNKQFSPLKDDLQYDSVTAPVRSVSYNGNLTPPHSKSDSDL 240
           NFSVRKV PNVE+TD  N+SNK+FSPLKD+LQYDS    VRS SYNG+LTPP +K D +L
Sbjct: 181 NFSVRKVPPNVESTDNHNTSNKEFSPLKDELQYDS----VRSGSYNGSLTPPQTKYDGNL 240

Query: 241 GEKKFGPAMLFLPRQPCEEDWANLVAATDSGFALTGTAAMGHVGPIIGLMDIGECEDSYL 300
            EKKFGPAMLFLPRQPCEEDWA+LVAA D+GFALTGTAAMGHVGPIIGLMDIGECEDSYL
Sbjct: 241 EEKKFGPAMLFLPRQPCEEDWASLVAANDTGFALTGTAAMGHVGPIIGLMDIGECEDSYL 300

Query: 301 FRVSLPGVKRDGRGFNCEVEKDGRVVIQGVTTTGERTVKKHSQVFEMLTHNLCPPGEFSL 360
           FRVSLPGVKRDG+ FNCEVEKDGRVVIQGVTTTGERTVKKHSQVFEM+THNLCPPGEFSL
Sbjct: 301 FRVSLPGVKRDGKEFNCEVEKDGRVVIQGVTTTGERTVKKHSQVFEMVTHNLCPPGEFSL 360

Query: 361 SFQLPGPVDPQQFFANFGIDGILEGVVMKDLQ 391
           SFQLPGPVDPQQF  NFGIDGILEG VMK+ Q
Sbjct: 361 SFQLPGPVDPQQFLVNFGIDGILEGAVMKEFQ 388

BLAST of Lag0006626 vs. NCBI nr
Match: XP_023521355.1 (increased DNA methylation 2-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 703.0 bits (1813), Expect = 1.5e-198
Identity = 341/392 (86.99%), Postives = 360/392 (91.84%), Query Frame = 0

Query: 1   MDGGFPQGSKSHIAVMPTKTPDGDQHFLLYFIAGIYFGPDLKGERPLKSALQRLAEGLPP 60
           MDG FPQ SKS + VMPTKTPDGDQHFLLY IAGIYFGPDLKGERPLKS LQRLA  LPP
Sbjct: 1   MDGSFPQDSKSPLTVMPTKTPDGDQHFLLYLIAGIYFGPDLKGERPLKSVLQRLAAALPP 60

Query: 61  YTSDQLAGSQMKMVEVERIFYYVLRKADESLVMKMSLLHQFFHGNFPAQGRDISFPQFPD 120
           YTSDQLAGSQ+K+VEVERIFYYVLRKADESL+MK SLLHQFF G FPAQGRDISFPQFPD
Sbjct: 61  YTSDQLAGSQIKVVEVERIFYYVLRKADESLIMKTSLLHQFFQGKFPAQGRDISFPQFPD 120

Query: 121 LFPPEFHPHSRSKNWYRFIENLSFIHYPEVYYLNSEDVERFKRLTGLDDFLLDRDAARSH 180
           LFPPE HPHSRSKNWYRFIENLSFIH P+V YLNSEDVERFKRLTGL+DFLLDRDAARSH
Sbjct: 121 LFPPELHPHSRSKNWYRFIENLSFIHNPDVSYLNSEDVERFKRLTGLNDFLLDRDAARSH 180

Query: 181 NFSVRKVSPNVEATD--NSSNKQFSPLKDDLQYDSVTAPVRSVSYNGNLTPPHSKSDSDL 240
           NFSVRKV PNVE+TD  N+SNK+FSPLKD+LQYDS    VRS SYNG+LTPP +K D +L
Sbjct: 181 NFSVRKVPPNVESTDNHNTSNKEFSPLKDELQYDS----VRSGSYNGSLTPPQTKYDCNL 240

Query: 241 GEKKFGPAMLFLPRQPCEEDWANLVAATDSGFALTGTAAMGHVGPIIGLMDIGECEDSYL 300
            EKKFGPAMLFLPRQPCEEDWA+L+AA D+GFALTGTAAMGHVGPIIGLMDIGECEDSYL
Sbjct: 241 EEKKFGPAMLFLPRQPCEEDWASLIAANDTGFALTGTAAMGHVGPIIGLMDIGECEDSYL 300

Query: 301 FRVSLPGVKRDGRGFNCEVEKDGRVVIQGVTTTGERTVKKHSQVFEMLTHNLCPPGEFSL 360
           FRVSLPGVKRDG+ FNCEVEKDGRVVIQGVTTTGERTVKKHSQVFEM+THNLCPPGEFSL
Sbjct: 301 FRVSLPGVKRDGKEFNCEVEKDGRVVIQGVTTTGERTVKKHSQVFEMVTHNLCPPGEFSL 360

Query: 361 SFQLPGPVDPQQFFANFGIDGILEGVVMKDLQ 391
           SFQLPGPVDPQQF  NFGIDGILEG VMK+ Q
Sbjct: 361 SFQLPGPVDPQQFLVNFGIDGILEGAVMKEFQ 388

BLAST of Lag0006626 vs. NCBI nr
Match: XP_023538073.1 (increased DNA methylation 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 700.7 bits (1807), Expect = 7.3e-198
Identity = 341/392 (86.99%), Postives = 359/392 (91.58%), Query Frame = 0

Query: 1   MDGGFPQGSKSHIAVMPTKTPDGDQHFLLYFIAGIYFGPDLKGERPLKSALQRLAEGLPP 60
           MDG FPQ SKS + VMPTKTPDGDQHFLLY IAGIYFGPDLKGERPLKS LQRLA  LPP
Sbjct: 1   MDGSFPQDSKSPLTVMPTKTPDGDQHFLLYLIAGIYFGPDLKGERPLKSVLQRLAAALPP 60

Query: 61  YTSDQLAGSQMKMVEVERIFYYVLRKADESLVMKMSLLHQFFHGNFPAQGRDISFPQFPD 120
           YTSDQLAGSQ+K+VEVERIFYYVLRKADESL+MK SLLHQFF G FPAQGRDISFPQFPD
Sbjct: 61  YTSDQLAGSQIKVVEVERIFYYVLRKADESLIMKTSLLHQFFQGKFPAQGRDISFPQFPD 120

Query: 121 LFPPEFHPHSRSKNWYRFIENLSFIHYPEVYYLNSEDVERFKRLTGLDDFLLDRDAARSH 180
           LFPPE HPHSRSKNWYRFIENLSFIH P+V YLNSEDVERFKRLTGL+DFLLDRDAARSH
Sbjct: 121 LFPPELHPHSRSKNWYRFIENLSFIHNPDVSYLNSEDVERFKRLTGLNDFLLDRDAARSH 180

Query: 181 NFSVRKVSPNVEATD--NSSNKQFSPLKDDLQYDSVTAPVRSVSYNGNLTPPHSKSDSDL 240
           NFSVRKV PNVE+TD  N+SNK+FSPLKD+LQYDS    VRS SYNG+LTPP +K D +L
Sbjct: 181 NFSVRKVPPNVESTDNHNTSNKEFSPLKDELQYDS----VRSGSYNGSLTPPQTKYDCNL 240

Query: 241 GEKKFGPAMLFLPRQPCEEDWANLVAATDSGFALTGTAAMGHVGPIIGLMDIGECEDSYL 300
            EKKFGPAMLFLPRQPCEEDWA+LVAA D+GFALTGTAAMGHVGPIIGLMDIGECEDSYL
Sbjct: 241 EEKKFGPAMLFLPRQPCEEDWASLVAANDTGFALTGTAAMGHVGPIIGLMDIGECEDSYL 300

Query: 301 FRVSLPGVKRDGRGFNCEVEKDGRVVIQGVTTTGERTVKKHSQVFEMLTHNLCPPGEFSL 360
           FRVSLPGVKR G+ FNCEVEKDGRVVIQGVTTTGERTVKKHSQVFEM+THNLCPPGEFSL
Sbjct: 301 FRVSLPGVKRGGKEFNCEVEKDGRVVIQGVTTTGERTVKKHSQVFEMVTHNLCPPGEFSL 360

Query: 361 SFQLPGPVDPQQFFANFGIDGILEGVVMKDLQ 391
           SFQLPGPVDPQQF  NFGIDGILEG VMK+ Q
Sbjct: 361 SFQLPGPVDPQQFLVNFGIDGILEGAVMKEFQ 388

BLAST of Lag0006626 vs. NCBI nr
Match: KAG7021118.1 (Increased DNA methylation 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 697.6 bits (1799), Expect = 6.2e-197
Identity = 339/392 (86.48%), Postives = 358/392 (91.33%), Query Frame = 0

Query: 1   MDGGFPQGSKSHIAVMPTKTPDGDQHFLLYFIAGIYFGPDLKGERPLKSALQRLAEGLPP 60
           MDG F Q SKS + VMPTKTPDGDQHFLLY IAGIYFGPDLKGERPLKS LQRLA  LPP
Sbjct: 1   MDGSFHQDSKSPLTVMPTKTPDGDQHFLLYLIAGIYFGPDLKGERPLKSVLQRLAAALPP 60

Query: 61  YTSDQLAGSQMKMVEVERIFYYVLRKADESLVMKMSLLHQFFHGNFPAQGRDISFPQFPD 120
           Y SDQLAGSQ+K+VEVERIFYYV+RKADESL+MK SLLHQFF G FPAQGRDISFPQFPD
Sbjct: 61  YASDQLAGSQIKVVEVERIFYYVMRKADESLIMKTSLLHQFFQGKFPAQGRDISFPQFPD 120

Query: 121 LFPPEFHPHSRSKNWYRFIENLSFIHYPEVYYLNSEDVERFKRLTGLDDFLLDRDAARSH 180
           LFPPE HPHSRSKNWYRFIENLSFIH P+V YLNSEDVERFKRLTGL+DFLLDRDAARSH
Sbjct: 121 LFPPELHPHSRSKNWYRFIENLSFIHNPDVSYLNSEDVERFKRLTGLNDFLLDRDAARSH 180

Query: 181 NFSVRKVSPNVEATD--NSSNKQFSPLKDDLQYDSVTAPVRSVSYNGNLTPPHSKSDSDL 240
           NFSVRKV PNVE+TD  N+SNK+FSPLKD+LQYDS    VRS SYNG+LTPP +K D +L
Sbjct: 181 NFSVRKVPPNVESTDNHNTSNKEFSPLKDELQYDS----VRSGSYNGSLTPPQTKYDGNL 240

Query: 241 GEKKFGPAMLFLPRQPCEEDWANLVAATDSGFALTGTAAMGHVGPIIGLMDIGECEDSYL 300
            EKKFGPAMLFLPRQPCEEDWA+LVAA D+GFALTGTAAMGHVGPIIGLMDIGECEDSYL
Sbjct: 241 EEKKFGPAMLFLPRQPCEEDWASLVAANDTGFALTGTAAMGHVGPIIGLMDIGECEDSYL 300

Query: 301 FRVSLPGVKRDGRGFNCEVEKDGRVVIQGVTTTGERTVKKHSQVFEMLTHNLCPPGEFSL 360
           FRVSLPGVKRDG+ FNCEVEKDGRVVIQGVTTTGERTVKKHSQVFEM+THNLCPPGEFSL
Sbjct: 301 FRVSLPGVKRDGKEFNCEVEKDGRVVIQGVTTTGERTVKKHSQVFEMVTHNLCPPGEFSL 360

Query: 361 SFQLPGPVDPQQFFANFGIDGILEGVVMKDLQ 391
           SFQLPGPVDPQQF  NFGIDGILEG VMK+ Q
Sbjct: 361 SFQLPGPVDPQQFLVNFGIDGILEGAVMKEFQ 388

BLAST of Lag0006626 vs. NCBI nr
Match: XP_022965797.1 (increased DNA methylation 2-like [Cucurbita maxima])

HSP 1 Score: 695.7 bits (1794), Expect = 2.3e-196
Identity = 338/392 (86.22%), Postives = 358/392 (91.33%), Query Frame = 0

Query: 1   MDGGFPQGSKSHIAVMPTKTPDGDQHFLLYFIAGIYFGPDLKGERPLKSALQRLAEGLPP 60
           MDG FPQ SKS + VMPTKTPDGDQHFLLY IAGIYFGPDLKGERPLKS LQRLA   PP
Sbjct: 1   MDGSFPQDSKSPLTVMPTKTPDGDQHFLLYLIAGIYFGPDLKGERPLKSVLQRLAAAFPP 60

Query: 61  YTSDQLAGSQMKMVEVERIFYYVLRKADESLVMKMSLLHQFFHGNFPAQGRDISFPQFPD 120
           YTSDQLAGSQ+K+VEVERIFYYVLRKADESL+MK SLLHQFF G FPAQGRDISFPQFPD
Sbjct: 61  YTSDQLAGSQIKVVEVERIFYYVLRKADESLIMKTSLLHQFFQGKFPAQGRDISFPQFPD 120

Query: 121 LFPPEFHPHSRSKNWYRFIENLSFIHYPEVYYLNSEDVERFKRLTGLDDFLLDRDAARSH 180
           LFPPE HPHSRSKNWYRFIENLSFIH P+V YLNSEDVERFKRLTGL+DFLLDRDAARSH
Sbjct: 121 LFPPELHPHSRSKNWYRFIENLSFIHNPDVSYLNSEDVERFKRLTGLNDFLLDRDAARSH 180

Query: 181 NFSVRKVSPNVEATD--NSSNKQFSPLKDDLQYDSVTAPVRSVSYNGNLTPPHSKSDSDL 240
           NFSVRKV  NVE+TD  N+SNK+FSPLKD+LQYDS    VRS SYNG+LTPP +K D +L
Sbjct: 181 NFSVRKVPSNVESTDNHNTSNKEFSPLKDELQYDS----VRSGSYNGSLTPPQTKYDCNL 240

Query: 241 GEKKFGPAMLFLPRQPCEEDWANLVAATDSGFALTGTAAMGHVGPIIGLMDIGECEDSYL 300
            EKKFGPAMLFLPRQPCEEDWA++VAA D+GFALTGTAAMGHVGPIIGLMDIGECEDSYL
Sbjct: 241 EEKKFGPAMLFLPRQPCEEDWASVVAANDTGFALTGTAAMGHVGPIIGLMDIGECEDSYL 300

Query: 301 FRVSLPGVKRDGRGFNCEVEKDGRVVIQGVTTTGERTVKKHSQVFEMLTHNLCPPGEFSL 360
           FRVSLPGVKR+G+ FNCEVEKDGRVVIQGVTTTGERTVKKHSQVFEM+THNLCPPGEFSL
Sbjct: 301 FRVSLPGVKRNGKEFNCEVEKDGRVVIQGVTTTGERTVKKHSQVFEMVTHNLCPPGEFSL 360

Query: 361 SFQLPGPVDPQQFFANFGIDGILEGVVMKDLQ 391
           SFQLPGPVDPQQF  NFGIDGILEG VMK+ Q
Sbjct: 361 SFQLPGPVDPQQFLVNFGIDGILEGAVMKEFQ 388

BLAST of Lag0006626 vs. ExPASy Swiss-Prot
Match: Q8RWL4 (Increased DNA methylation 2 OS=Arabidopsis thaliana OX=3702 GN=IDM2 PE=1 SV=1)

HSP 1 Score: 268.9 bits (686), Expect = 9.3e-71
Identity = 158/369 (42.82%), Postives = 214/369 (57.99%), Query Frame = 0

Query: 24  DQHFLLYFIAGIYFGPDLKGE--RPLKSALQRLAEGLPPYTSDQLAGSQMKMVEVERIFY 83
           D+ FLL+FI G YFGPDL+ +  RP +SA Q   + L     D+L+GS MK  E+ER++Y
Sbjct: 33  DKLFLLHFIIGTYFGPDLRKQHHRPKQSAFQ--IQALKNVVVDELSGSLMKRAELERVYY 92

Query: 84  YVLRKADESLVMKMSLLHQFFHGNFPAQGRDISFPQFPDLFPPEFHPHSRSKNWYRFIEN 143
           +++R  D SLVMK   L ++F+       RD  +P F DLFP + HP +  ++ ++FI +
Sbjct: 93  HIIRNVDPSLVMKPKKLREYFNAKRNDSNRD--YPLFVDLFPRKLHPETHVRHKFKFIRS 152

Query: 144 LSFIHYPEVYYLNSEDVERFKRLTGLDDFLLDRDAARSHNFSVRKVSPNVEATDNSSNKQ 203
           + FI+ P+   +  E V RFKRLTGLD F L               S +V+ T   SN  
Sbjct: 153 IVFINDPDTSCMREECVARFKRLTGLDSFAL---------------SLSVDVT--KSNGV 212

Query: 204 FSPLKDDLQYDSVTAPVRSVSYNGNLTPPHSKSDSDLGEKKFGPAMLFLPRQPCEEDWAN 263
            +  +  ++ D    PV+      N     S  +SD+  K         P    E     
Sbjct: 213 VAANEVKVEIDESVEPVK----EDNAGTCTSGEESDVAAK---------PEVKSE----- 272

Query: 264 LVAATDSGFALTGTAAMGHVGPIIGLMDIGECEDSYLFRVSLPGVKRDGRGFNCEVEKDG 323
                             H G ++GLMDIGEC+D+YLFRVSLPGVKRD R F+CEVE +G
Sbjct: 273 -----------------AHGGLMVGLMDIGECDDAYLFRVSLPGVKRDERYFSCEVEDNG 332

Query: 324 RVVIQGVTTTGERTVKKHSQVFEMLTHNLCPPGEFSLSFQLPGPVDPQQFFANFGIDGIL 383
           +V+++GVTTTG + VK++S VFEM T +LCPPG FS+SF+LPGPV P +F  NFG DGIL
Sbjct: 333 KVLVRGVTTTGGKRVKRYSHVFEMQTRSLCPPGNFSVSFRLPGPVHPHEFSGNFGTDGIL 345

Query: 384 EGVVMKDLQ 391
           EGVVMK+LQ
Sbjct: 393 EGVVMKNLQ 345

BLAST of Lag0006626 vs. ExPASy Swiss-Prot
Match: Q9SYQ0 (Increased DNA methylation 3 OS=Arabidopsis thaliana OX=3702 GN=IDM3 PE=1 SV=1)

HSP 1 Score: 212.6 bits (540), Expect = 7.9e-54
Identity = 136/454 (29.96%), Postives = 212/454 (46.70%), Query Frame = 0

Query: 24  DQHFLLYFIAGIYFGPDLKGERPLKSALQRLAEGLPPYTSDQLAGSQMKMVEVERIFYYV 83
           DQ+FL+ FI   Y GPD+  + P  S+ QRLA GLPPYT   +  S + + +++ ++Y V
Sbjct: 11  DQYFLVNFIMSNYLGPDVYSDNPRCSSSQRLARGLPPYTLMHIGSSSLTVSQLQNLYYNV 70

Query: 84  LRKADESLVMKMSLLHQFFHGNFPAQGRDISFPQFPDLFPPEFHPHSRSKNWYRFIENLS 143
           LR A  SL++   +++ +  G  P +     FPQF   FP   HP  R    +  ++ + 
Sbjct: 71  LRNAKSSLLLHPDMIYMYLKGYLPLEPSG-KFPQFTHFFPTNLHPQKRYSPSHEIVKGIV 130

Query: 144 FIHYPEVYYLNSEDVERFKRLTGLDDFLLDRDAARSHNFSV-------RKVSPNVEATDN 203
            I  P V ++N E+++RF+ L+ LDD  +DR  + S   ++       +  S N +AT N
Sbjct: 131 VIDDPAVGFINKEELQRFRCLSRLDDLKIDRVTSLSPRVNLDESRETEQDCSRNGDATAN 190

Query: 204 ------------------------------------------------------SSNKQF 263
                                                                 +    F
Sbjct: 191 GVVTNEDYNSSGELQETCKRKEGEDAVASCVISEPERLSGDIPESQGMKQDCSRNGESAF 250

Query: 264 SPLKDDLQYDS-VTAP------------VRSVSYNGNLTPPHS----------KSDSDLG 323
           S +  D  Y S V  P            V   + +G    P            K+ S   
Sbjct: 251 SGIVSDQDYYSFVKLPETCKRKNKEEEAVTGHAVSGTSKTPERFRETYKRRRFKNSSKKA 310

Query: 324 EKKFGPAMLFLPRQPCEEDWANLVAATDS--GFALTGTAAMGHVGPIIGLMDIGECEDSY 383
             K G  ++   +      +++ +  +D+      TGTA+   +G  +G++DIG  + +Y
Sbjct: 311 TNKNGETLMEREKTDKPIPFSSEMKESDAEPSVVTTGTASKETLGSSVGVVDIGVNKVAY 370

Query: 384 LFRVSLPGVKRDGRGFNCEVEKDGRVVIQGVTTTGERTVKKHSQVFEMLTHNLCPPGEFS 392
            F+V+LPGV++D   F+CE+E DG+V+++G TT GE+ +K+HS+VFEM    LCPPG F 
Sbjct: 371 FFQVALPGVRKDYGEFSCEIESDGKVILEGSTTRGEKNIKRHSRVFEMNIRKLCPPGPFK 430

BLAST of Lag0006626 vs. ExPASy Swiss-Prot
Match: Q84K79 (Alpha-crystallin domain-containing protein 22.3 OS=Arabidopsis thaliana OX=3702 GN=ACD22.3 PE=1 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 1.1e-50
Identity = 97/173 (56.07%), Postives = 129/173 (74.57%), Query Frame = 0

Query: 217 PVRSVSYNGNLTPPHSKSD--SDLGEKKFGPAMLFLPRQPCEEDWANLVAATDSGFALTG 276
           P+ S+ Y G +T     S+  +D  EK  GPAM+FLP     E ++NL++ T +G ALTG
Sbjct: 30  PLNSMPYIGPVTHASMSSNRMNDSVEKVGGPAMIFLPSDSSSE-FSNLISQTKTGVALTG 89

Query: 277 TAAMGHVGPIIGLMDIGECEDSYLFRVSLPGVKRDGRGFNCEVEKDGRVVIQGVTTTGER 336
           +AAMG +G  IGL+DI E EDSY FRV+LPGV RD + F+CE+E DG+++I+G TTTGE+
Sbjct: 90  SAAMGKIGLTIGLVDIAESEDSYYFRVALPGVSRDEKEFSCEIEPDGKIMIKGATTTGEQ 149

Query: 337 TVKKHSQVFEMLTHNLCPPGEFSLSFQLPGPVDPQQFFANFGIDGILEGVVMK 388
           TV KH+Q+F+MLT NLCPPG F+++FQLPGPV  ++F  NFG DG+LEGVV K
Sbjct: 150 TVCKHNQIFKMLTQNLCPPGHFTINFQLPGPVSNEEFNGNFGSDGVLEGVVKK 201

BLAST of Lag0006626 vs. ExPASy TrEMBL
Match: A0A6J1FC97 (increased DNA methylation 2-like OS=Cucurbita moschata OX=3662 GN=LOC111444304 PE=3 SV=1)

HSP 1 Score: 703.0 bits (1813), Expect = 7.1e-199
Identity = 341/392 (86.99%), Postives = 360/392 (91.84%), Query Frame = 0

Query: 1   MDGGFPQGSKSHIAVMPTKTPDGDQHFLLYFIAGIYFGPDLKGERPLKSALQRLAEGLPP 60
           MDG FPQ SKS + VMPTKTPDGDQHFLLY IAGIYFGPDLKGERP+KS LQRLA  LPP
Sbjct: 1   MDGSFPQDSKSPLTVMPTKTPDGDQHFLLYLIAGIYFGPDLKGERPMKSVLQRLAAALPP 60

Query: 61  YTSDQLAGSQMKMVEVERIFYYVLRKADESLVMKMSLLHQFFHGNFPAQGRDISFPQFPD 120
           YTSDQLAGSQ+K+VEVERIFYYVLRKADESL+MK SLLHQFF G FPAQGRDISFPQFPD
Sbjct: 61  YTSDQLAGSQIKVVEVERIFYYVLRKADESLIMKTSLLHQFFQGKFPAQGRDISFPQFPD 120

Query: 121 LFPPEFHPHSRSKNWYRFIENLSFIHYPEVYYLNSEDVERFKRLTGLDDFLLDRDAARSH 180
           LFPPE HPHSRSKNWYRFIENLSFIH P+V YLNSEDVERFKRLTGL+DFLLDRDAARSH
Sbjct: 121 LFPPELHPHSRSKNWYRFIENLSFIHNPDVSYLNSEDVERFKRLTGLNDFLLDRDAARSH 180

Query: 181 NFSVRKVSPNVEATD--NSSNKQFSPLKDDLQYDSVTAPVRSVSYNGNLTPPHSKSDSDL 240
           NFSVRKV PNVE+TD  N+SNK+FSPLKD+LQYDS    VRS SYNG+LTPP +K D +L
Sbjct: 181 NFSVRKVPPNVESTDNHNTSNKEFSPLKDELQYDS----VRSGSYNGSLTPPQTKYDGNL 240

Query: 241 GEKKFGPAMLFLPRQPCEEDWANLVAATDSGFALTGTAAMGHVGPIIGLMDIGECEDSYL 300
            EKKFGPAMLFLPRQPCEEDWA+LVAA D+GFALTGTAAMGHVGPIIGLMDIGECEDSYL
Sbjct: 241 EEKKFGPAMLFLPRQPCEEDWASLVAANDTGFALTGTAAMGHVGPIIGLMDIGECEDSYL 300

Query: 301 FRVSLPGVKRDGRGFNCEVEKDGRVVIQGVTTTGERTVKKHSQVFEMLTHNLCPPGEFSL 360
           FRVSLPGVKRDG+ FNCEVEKDGRVVIQGVTTTGERTVKKHSQVFEM+THNLCPPGEFSL
Sbjct: 301 FRVSLPGVKRDGKEFNCEVEKDGRVVIQGVTTTGERTVKKHSQVFEMVTHNLCPPGEFSL 360

Query: 361 SFQLPGPVDPQQFFANFGIDGILEGVVMKDLQ 391
           SFQLPGPVDPQQF  NFGIDGILEG VMK+ Q
Sbjct: 361 SFQLPGPVDPQQFLVNFGIDGILEGAVMKEFQ 388

BLAST of Lag0006626 vs. ExPASy TrEMBL
Match: A0A6J1HLA0 (increased DNA methylation 2-like OS=Cucurbita maxima OX=3661 GN=LOC111465577 PE=3 SV=1)

HSP 1 Score: 695.7 bits (1794), Expect = 1.1e-196
Identity = 338/392 (86.22%), Postives = 358/392 (91.33%), Query Frame = 0

Query: 1   MDGGFPQGSKSHIAVMPTKTPDGDQHFLLYFIAGIYFGPDLKGERPLKSALQRLAEGLPP 60
           MDG FPQ SKS + VMPTKTPDGDQHFLLY IAGIYFGPDLKGERPLKS LQRLA   PP
Sbjct: 1   MDGSFPQDSKSPLTVMPTKTPDGDQHFLLYLIAGIYFGPDLKGERPLKSVLQRLAAAFPP 60

Query: 61  YTSDQLAGSQMKMVEVERIFYYVLRKADESLVMKMSLLHQFFHGNFPAQGRDISFPQFPD 120
           YTSDQLAGSQ+K+VEVERIFYYVLRKADESL+MK SLLHQFF G FPAQGRDISFPQFPD
Sbjct: 61  YTSDQLAGSQIKVVEVERIFYYVLRKADESLIMKTSLLHQFFQGKFPAQGRDISFPQFPD 120

Query: 121 LFPPEFHPHSRSKNWYRFIENLSFIHYPEVYYLNSEDVERFKRLTGLDDFLLDRDAARSH 180
           LFPPE HPHSRSKNWYRFIENLSFIH P+V YLNSEDVERFKRLTGL+DFLLDRDAARSH
Sbjct: 121 LFPPELHPHSRSKNWYRFIENLSFIHNPDVSYLNSEDVERFKRLTGLNDFLLDRDAARSH 180

Query: 181 NFSVRKVSPNVEATD--NSSNKQFSPLKDDLQYDSVTAPVRSVSYNGNLTPPHSKSDSDL 240
           NFSVRKV  NVE+TD  N+SNK+FSPLKD+LQYDS    VRS SYNG+LTPP +K D +L
Sbjct: 181 NFSVRKVPSNVESTDNHNTSNKEFSPLKDELQYDS----VRSGSYNGSLTPPQTKYDCNL 240

Query: 241 GEKKFGPAMLFLPRQPCEEDWANLVAATDSGFALTGTAAMGHVGPIIGLMDIGECEDSYL 300
            EKKFGPAMLFLPRQPCEEDWA++VAA D+GFALTGTAAMGHVGPIIGLMDIGECEDSYL
Sbjct: 241 EEKKFGPAMLFLPRQPCEEDWASVVAANDTGFALTGTAAMGHVGPIIGLMDIGECEDSYL 300

Query: 301 FRVSLPGVKRDGRGFNCEVEKDGRVVIQGVTTTGERTVKKHSQVFEMLTHNLCPPGEFSL 360
           FRVSLPGVKR+G+ FNCEVEKDGRVVIQGVTTTGERTVKKHSQVFEM+THNLCPPGEFSL
Sbjct: 301 FRVSLPGVKRNGKEFNCEVEKDGRVVIQGVTTTGERTVKKHSQVFEMVTHNLCPPGEFSL 360

Query: 361 SFQLPGPVDPQQFFANFGIDGILEGVVMKDLQ 391
           SFQLPGPVDPQQF  NFGIDGILEG VMK+ Q
Sbjct: 361 SFQLPGPVDPQQFLVNFGIDGILEGAVMKEFQ 388

BLAST of Lag0006626 vs. ExPASy TrEMBL
Match: A0A5A7TYA7 (Increased DNA methylation 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold385G00080 PE=4 SV=1)

HSP 1 Score: 682.6 bits (1760), Expect = 1.0e-192
Identity = 335/391 (85.68%), Postives = 351/391 (89.77%), Query Frame = 0

Query: 1   MDGGFPQGSKSHIAVMPTKTPDGDQHFLLYFIAGIYFGPDLKGERPLKSALQRLAEGLPP 60
           MDG  PQ +KS I V+PTK PD DQHFLLYFIAGIYFGP+LKG+   KSALQRLAE LPP
Sbjct: 1   MDGSSPQNTKSPIPVVPTKAPDNDQHFLLYFIAGIYFGPNLKGKPAPKSALQRLAEELPP 60

Query: 61  YTSDQLAGSQMKMVEVERIFYYVLRKADESLVMKMSLLHQFFHGNFPAQGRDISFPQFPD 120
           YTSDQLAGS MKMVEVERIFYYVLRKADESL+MKMSLLHQFF G FPAQGRDIS PQFPD
Sbjct: 61  YTSDQLAGSLMKMVEVERIFYYVLRKADESLIMKMSLLHQFFQGKFPAQGRDISSPQFPD 120

Query: 121 LFPPEFHPHSRSKNWYRFIENLSFIHYPEVYYLNSEDVERFKRLTGLDDFLLDRDAARSH 180
           LFP E HPH+RSKNWYR+IENL FI+ PEVYYLN EDVERFKRLTGLDDF LDRDAA SH
Sbjct: 121 LFPLELHPHTRSKNWYRYIENLLFINNPEVYYLNPEDVERFKRLTGLDDFFLDRDAATSH 180

Query: 181 NFSVRKVSPNVEATDNSSNKQFSPLKDDLQYDSVTAPVRSVSYNGNLTPPHSKSDSDLGE 240
           N S RK S NVEAT+N SNK+FSPLKDD Q+D VT+PVRSV YNGNLTPPH+ SDS+L E
Sbjct: 181 NSSARKASLNVEATENRSNKEFSPLKDDQQHDLVTSPVRSVPYNGNLTPPHTNSDSNLLE 240

Query: 241 KKFGPAMLFLPRQPCEEDWANLVAATDSGFALTGTAAMGHVGPIIGLMDIGECEDSYLFR 300
           KKFGPAMLFLPRQP EEDWANLVAAT+SGFALTGTAAMGHVGPIIG MDIGECEDSYLFR
Sbjct: 241 KKFGPAMLFLPRQPSEEDWANLVAATNSGFALTGTAAMGHVGPIIGSMDIGECEDSYLFR 300

Query: 301 VSLPGVKRDGRGFNCEVEKDGRVVIQGVTTTGERTVKKHSQVFEMLTHNLCPPGEFSLSF 360
           VSLPGVKRD  GFNCEVEKDGRVVIQGVTTTGERTVKKHSQVFEM+THNLCPPGEFSLSF
Sbjct: 301 VSLPGVKRDPCGFNCEVEKDGRVVIQGVTTTGERTVKKHSQVFEMVTHNLCPPGEFSLSF 360

Query: 361 QLPGPVDPQQFFANFGIDGILEGVVMKDLQS 392
           QLPGPVDPQ F ANF I GILEGVVMKDLQS
Sbjct: 361 QLPGPVDPQHFLANFDIAGILEGVVMKDLQS 391

BLAST of Lag0006626 vs. ExPASy TrEMBL
Match: A0A1S3BZJ4 (increased DNA methylation 2 OS=Cucumis melo OX=3656 GN=LOC103495173 PE=4 SV=1)

HSP 1 Score: 682.6 bits (1760), Expect = 1.0e-192
Identity = 335/391 (85.68%), Postives = 351/391 (89.77%), Query Frame = 0

Query: 1   MDGGFPQGSKSHIAVMPTKTPDGDQHFLLYFIAGIYFGPDLKGERPLKSALQRLAEGLPP 60
           MDG  PQ +KS I V+PTK PD DQHFLLYFIAGIYFGP+LKG+   KSALQRLAE LPP
Sbjct: 1   MDGSSPQNTKSPIPVVPTKAPDNDQHFLLYFIAGIYFGPNLKGKPAPKSALQRLAEELPP 60

Query: 61  YTSDQLAGSQMKMVEVERIFYYVLRKADESLVMKMSLLHQFFHGNFPAQGRDISFPQFPD 120
           YTSDQLAGS MKMVEVERIFYYVLRKADESL+MKMSLLHQFF G FPAQGRDIS PQFPD
Sbjct: 61  YTSDQLAGSLMKMVEVERIFYYVLRKADESLIMKMSLLHQFFQGKFPAQGRDISSPQFPD 120

Query: 121 LFPPEFHPHSRSKNWYRFIENLSFIHYPEVYYLNSEDVERFKRLTGLDDFLLDRDAARSH 180
           LFP E HPH+RSKNWYR+IENL FI+ PEVYYLN EDVERFKRLTGLDDF LDRDAA SH
Sbjct: 121 LFPLELHPHTRSKNWYRYIENLLFINNPEVYYLNPEDVERFKRLTGLDDFFLDRDAATSH 180

Query: 181 NFSVRKVSPNVEATDNSSNKQFSPLKDDLQYDSVTAPVRSVSYNGNLTPPHSKSDSDLGE 240
           N S RK S NVEAT+N SNK+FSPLKDD Q+D VT+PVRSV YNGNLTPPH+ SDS+L E
Sbjct: 181 NSSARKASLNVEATENRSNKEFSPLKDDQQHDLVTSPVRSVPYNGNLTPPHTNSDSNLLE 240

Query: 241 KKFGPAMLFLPRQPCEEDWANLVAATDSGFALTGTAAMGHVGPIIGLMDIGECEDSYLFR 300
           KKFGPAMLFLPRQP EEDWANLVAAT+SGFALTGTAAMGHVGPIIG MDIGECEDSYLFR
Sbjct: 241 KKFGPAMLFLPRQPSEEDWANLVAATNSGFALTGTAAMGHVGPIIGSMDIGECEDSYLFR 300

Query: 301 VSLPGVKRDGRGFNCEVEKDGRVVIQGVTTTGERTVKKHSQVFEMLTHNLCPPGEFSLSF 360
           VSLPGVKRD  GFNCEVEKDGRVVIQGVTTTGERTVKKHSQVFEM+THNLCPPGEFSLSF
Sbjct: 301 VSLPGVKRDPCGFNCEVEKDGRVVIQGVTTTGERTVKKHSQVFEMVTHNLCPPGEFSLSF 360

Query: 361 QLPGPVDPQQFFANFGIDGILEGVVMKDLQS 392
           QLPGPVDPQ F ANF I GILEGVVMKDLQS
Sbjct: 361 QLPGPVDPQHFLANFDIAGILEGVVMKDLQS 391

BLAST of Lag0006626 vs. ExPASy TrEMBL
Match: Q84KA7 (Heat-shock protein-like protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1)

HSP 1 Score: 681.8 bits (1758), Expect = 1.7e-192
Identity = 335/391 (85.68%), Postives = 350/391 (89.51%), Query Frame = 0

Query: 1   MDGGFPQGSKSHIAVMPTKTPDGDQHFLLYFIAGIYFGPDLKGERPLKSALQRLAEGLPP 60
           MDG  PQ +KS I V+PTK PD DQHFLLYFIAGIYFGP LKG+   KSALQRLAE LPP
Sbjct: 1   MDGSSPQNTKSPIPVVPTKAPDNDQHFLLYFIAGIYFGPTLKGKPAPKSALQRLAEELPP 60

Query: 61  YTSDQLAGSQMKMVEVERIFYYVLRKADESLVMKMSLLHQFFHGNFPAQGRDISFPQFPD 120
           YTSDQLAGS MKMVEVERIFYYVLRKADESL+MKMSLLHQFF G FPAQGRDIS PQFPD
Sbjct: 61  YTSDQLAGSLMKMVEVERIFYYVLRKADESLIMKMSLLHQFFQGKFPAQGRDISSPQFPD 120

Query: 121 LFPPEFHPHSRSKNWYRFIENLSFIHYPEVYYLNSEDVERFKRLTGLDDFLLDRDAARSH 180
           LFP E HPH+RSKNWYR+IENL FI+ PEVYYLN EDVERFKRLTGLDDF LDRDAA SH
Sbjct: 121 LFPLELHPHTRSKNWYRYIENLLFINNPEVYYLNPEDVERFKRLTGLDDFFLDRDAATSH 180

Query: 181 NFSVRKVSPNVEATDNSSNKQFSPLKDDLQYDSVTAPVRSVSYNGNLTPPHSKSDSDLGE 240
           N S RK S NVEAT+N SNK+FSPLKDD Q+D VT+PVRSV YNGNLTPPH+ SDS+L E
Sbjct: 181 NSSARKASLNVEATENRSNKEFSPLKDDQQHDLVTSPVRSVPYNGNLTPPHTNSDSNLLE 240

Query: 241 KKFGPAMLFLPRQPCEEDWANLVAATDSGFALTGTAAMGHVGPIIGLMDIGECEDSYLFR 300
           KKFGPAMLFLPRQP EEDWANLVAAT+SGFALTGTAAMGHVGPIIG MDIGECEDSYLFR
Sbjct: 241 KKFGPAMLFLPRQPSEEDWANLVAATNSGFALTGTAAMGHVGPIIGSMDIGECEDSYLFR 300

Query: 301 VSLPGVKRDGRGFNCEVEKDGRVVIQGVTTTGERTVKKHSQVFEMLTHNLCPPGEFSLSF 360
           VSLPGVKRD  GFNCEVEKDGRVVIQGVTTTGERTVKKHSQVFEM+THNLCPPGEFSLSF
Sbjct: 301 VSLPGVKRDPCGFNCEVEKDGRVVIQGVTTTGERTVKKHSQVFEMVTHNLCPPGEFSLSF 360

Query: 361 QLPGPVDPQQFFANFGIDGILEGVVMKDLQS 392
           QLPGPVDPQ F ANF I GILEGVVMKDLQS
Sbjct: 361 QLPGPVDPQHFLANFDIAGILEGVVMKDLQS 391

BLAST of Lag0006626 vs. TAIR 10
Match: AT1G54840.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 268.9 bits (686), Expect = 6.6e-72
Identity = 158/369 (42.82%), Postives = 214/369 (57.99%), Query Frame = 0

Query: 24  DQHFLLYFIAGIYFGPDLKGE--RPLKSALQRLAEGLPPYTSDQLAGSQMKMVEVERIFY 83
           D+ FLL+FI G YFGPDL+ +  RP +SA Q   + L     D+L+GS MK  E+ER++Y
Sbjct: 33  DKLFLLHFIIGTYFGPDLRKQHHRPKQSAFQ--IQALKNVVVDELSGSLMKRAELERVYY 92

Query: 84  YVLRKADESLVMKMSLLHQFFHGNFPAQGRDISFPQFPDLFPPEFHPHSRSKNWYRFIEN 143
           +++R  D SLVMK   L ++F+       RD  +P F DLFP + HP +  ++ ++FI +
Sbjct: 93  HIIRNVDPSLVMKPKKLREYFNAKRNDSNRD--YPLFVDLFPRKLHPETHVRHKFKFIRS 152

Query: 144 LSFIHYPEVYYLNSEDVERFKRLTGLDDFLLDRDAARSHNFSVRKVSPNVEATDNSSNKQ 203
           + FI+ P+   +  E V RFKRLTGLD F L               S +V+ T   SN  
Sbjct: 153 IVFINDPDTSCMREECVARFKRLTGLDSFAL---------------SLSVDVT--KSNGV 212

Query: 204 FSPLKDDLQYDSVTAPVRSVSYNGNLTPPHSKSDSDLGEKKFGPAMLFLPRQPCEEDWAN 263
            +  +  ++ D    PV+      N     S  +SD+  K         P    E     
Sbjct: 213 VAANEVKVEIDESVEPVK----EDNAGTCTSGEESDVAAK---------PEVKSE----- 272

Query: 264 LVAATDSGFALTGTAAMGHVGPIIGLMDIGECEDSYLFRVSLPGVKRDGRGFNCEVEKDG 323
                             H G ++GLMDIGEC+D+YLFRVSLPGVKRD R F+CEVE +G
Sbjct: 273 -----------------AHGGLMVGLMDIGECDDAYLFRVSLPGVKRDERYFSCEVEDNG 332

Query: 324 RVVIQGVTTTGERTVKKHSQVFEMLTHNLCPPGEFSLSFQLPGPVDPQQFFANFGIDGIL 383
           +V+++GVTTTG + VK++S VFEM T +LCPPG FS+SF+LPGPV P +F  NFG DGIL
Sbjct: 333 KVLVRGVTTTGGKRVKRYSHVFEMQTRSLCPPGNFSVSFRLPGPVHPHEFSGNFGTDGIL 345

Query: 384 EGVVMKDLQ 391
           EGVVMK+LQ
Sbjct: 393 EGVVMKNLQ 345

BLAST of Lag0006626 vs. TAIR 10
Match: AT1G20870.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 212.6 bits (540), Expect = 5.6e-55
Identity = 136/454 (29.96%), Postives = 212/454 (46.70%), Query Frame = 0

Query: 24  DQHFLLYFIAGIYFGPDLKGERPLKSALQRLAEGLPPYTSDQLAGSQMKMVEVERIFYYV 83
           DQ+FL+ FI   Y GPD+  + P  S+ QRLA GLPPYT   +  S + + +++ ++Y V
Sbjct: 11  DQYFLVNFIMSNYLGPDVYSDNPRCSSSQRLARGLPPYTLMHIGSSSLTVSQLQNLYYNV 70

Query: 84  LRKADESLVMKMSLLHQFFHGNFPAQGRDISFPQFPDLFPPEFHPHSRSKNWYRFIENLS 143
           LR A  SL++   +++ +  G  P +     FPQF   FP   HP  R    +  ++ + 
Sbjct: 71  LRNAKSSLLLHPDMIYMYLKGYLPLEPSG-KFPQFTHFFPTNLHPQKRYSPSHEIVKGIV 130

Query: 144 FIHYPEVYYLNSEDVERFKRLTGLDDFLLDRDAARSHNFSV-------RKVSPNVEATDN 203
            I  P V ++N E+++RF+ L+ LDD  +DR  + S   ++       +  S N +AT N
Sbjct: 131 VIDDPAVGFINKEELQRFRCLSRLDDLKIDRVTSLSPRVNLDESRETEQDCSRNGDATAN 190

Query: 204 ------------------------------------------------------SSNKQF 263
                                                                 +    F
Sbjct: 191 GVVTNEDYNSSGELQETCKRKEGEDAVASCVISEPERLSGDIPESQGMKQDCSRNGESAF 250

Query: 264 SPLKDDLQYDS-VTAP------------VRSVSYNGNLTPPHS----------KSDSDLG 323
           S +  D  Y S V  P            V   + +G    P            K+ S   
Sbjct: 251 SGIVSDQDYYSFVKLPETCKRKNKEEEAVTGHAVSGTSKTPERFRETYKRRRFKNSSKKA 310

Query: 324 EKKFGPAMLFLPRQPCEEDWANLVAATDS--GFALTGTAAMGHVGPIIGLMDIGECEDSY 383
             K G  ++   +      +++ +  +D+      TGTA+   +G  +G++DIG  + +Y
Sbjct: 311 TNKNGETLMEREKTDKPIPFSSEMKESDAEPSVVTTGTASKETLGSSVGVVDIGVNKVAY 370

Query: 384 LFRVSLPGVKRDGRGFNCEVEKDGRVVIQGVTTTGERTVKKHSQVFEMLTHNLCPPGEFS 392
            F+V+LPGV++D   F+CE+E DG+V+++G TT GE+ +K+HS+VFEM    LCPPG F 
Sbjct: 371 FFQVALPGVRKDYGEFSCEIESDGKVILEGSTTRGEKNIKRHSRVFEMNIRKLCPPGPFK 430

BLAST of Lag0006626 vs. TAIR 10
Match: AT1G54850.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 202.2 bits (513), Expect = 7.6e-52
Identity = 97/173 (56.07%), Postives = 129/173 (74.57%), Query Frame = 0

Query: 217 PVRSVSYNGNLTPPHSKSD--SDLGEKKFGPAMLFLPRQPCEEDWANLVAATDSGFALTG 276
           P+ S+ Y G +T     S+  +D  EK  GPAM+FLP     E ++NL++ T +G ALTG
Sbjct: 30  PLNSMPYIGPVTHASMSSNRMNDSVEKVGGPAMIFLPSDSSSE-FSNLISQTKTGVALTG 89

Query: 277 TAAMGHVGPIIGLMDIGECEDSYLFRVSLPGVKRDGRGFNCEVEKDGRVVIQGVTTTGER 336
           +AAMG +G  IGL+DI E EDSY FRV+LPGV RD + F+CE+E DG+++I+G TTTGE+
Sbjct: 90  SAAMGKIGLTIGLVDIAESEDSYYFRVALPGVSRDEKEFSCEIEPDGKIMIKGATTTGEQ 149

Query: 337 TVKKHSQVFEMLTHNLCPPGEFSLSFQLPGPVDPQQFFANFGIDGILEGVVMK 388
           TV KH+Q+F+MLT NLCPPG F+++FQLPGPV  ++F  NFG DG+LEGVV K
Sbjct: 150 TVCKHNQIFKMLTQNLCPPGHFTINFQLPGPVSNEEFNGNFGSDGVLEGVVKK 201

BLAST of Lag0006626 vs. TAIR 10
Match: AT1G54840.2 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 155.2 bits (391), Expect = 1.1e-37
Identity = 105/288 (36.46%), Postives = 147/288 (51.04%), Query Frame = 0

Query: 24  DQHFLLYFIAGIYFGPDLKGE--RPLKSALQRLAEGLPPYTSDQLAGSQMKMVEVERIFY 83
           D+ FLL+FI G YFGPDL+ +  RP +SA Q   + L     D+L+GS MK  E+ER++Y
Sbjct: 33  DKLFLLHFIIGTYFGPDLRKQHHRPKQSAFQ--IQALKNVVVDELSGSLMKRAELERVYY 92

Query: 84  YVLRKADESLVMKMSLLHQFFHGNFPAQGRDISFPQFPDLFPPEFHPHSRSKNWYRFIEN 143
           +++R  D SLVMK   L ++F+       RD  +P F DLFP + HP +  ++ ++FI +
Sbjct: 93  HIIRNVDPSLVMKPKKLREYFNAKRNDSNRD--YPLFVDLFPRKLHPETHVRHKFKFIRS 152

Query: 144 LSFIHYPEVYYLNSEDVERFKRLTGLDDFLLDRDAARSHNFSVRKVSPNVEATDNSSNKQ 203
           + FI+ P+   +  E V RFKRLTGLD F L               S +V+ T   SN  
Sbjct: 153 IVFINDPDTSCMREECVARFKRLTGLDSFAL---------------SLSVDVT--KSNGV 212

Query: 204 FSPLKDDLQYDSVTAPVRSVSYNGNLTPPHSKSDSDLGEKKFGPAMLFLPRQPCEEDWAN 263
            +  +  ++ D    PV+      N     S  +SD+  K         P    E     
Sbjct: 213 VAANEVKVEIDESVEPVK----EDNAGTCTSGEESDVAAK---------PEVKSE----- 264

Query: 264 LVAATDSGFALTGTAAMGHVGPIIGLMDIGECEDSYLFRVSLPGVKRD 310
                             H G ++GLMDIGEC+D+YLFRVSLPGVKRD
Sbjct: 273 -----------------AHGGLMVGLMDIGECDDAYLFRVSLPGVKRD 264

BLAST of Lag0006626 vs. TAIR 10
Match: AT1G76440.1 (HSP20-like chaperones superfamily protein )

HSP 1 Score: 116.3 bits (290), Expect = 5.5e-26
Identity = 58/116 (50.00%), Postives = 78/116 (67.24%), Query Frame = 0

Query: 273 TGTAAMGHVGPIIGLMDIGECEDSYLFRVSLPGVKRDGRGFNCEVEKDGRVVIQGVTTTG 332
           TGTA +G VGP IGL+DIG  E +Y+FRVSLPG++++     CE++++GRV IQGV    
Sbjct: 22  TGTAKLGSVGPPIGLVDIGVSEVAYIFRVSLPGIEKNQDKIKCEIQREGRVCIQGVIP-- 81

Query: 333 ERTVKKHSQ-VFEMLTHNLCPPGEFSLSFQLPGPVDPQQFFANFGIDGILEGVVMK 388
           E  +   +  ++ M    LCPPG FS++F LPG VDP+ F  NF  DGI E VV+K
Sbjct: 82  EIAIPSDTGCLYRMQVQQLCPPGPFSITFNLPGQVDPRLFSPNFRSDGIFEVVVVK 135

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022938125.11.5e-19886.99increased DNA methylation 2-like [Cucurbita moschata] >XP_022938126.1 increased ... [more]
XP_023521355.11.5e-19886.99increased DNA methylation 2-like isoform X1 [Cucurbita pepo subsp. pepo][more]
XP_023538073.17.3e-19886.99increased DNA methylation 2-like [Cucurbita pepo subsp. pepo][more]
KAG7021118.16.2e-19786.48Increased DNA methylation 2 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022965797.12.3e-19686.22increased DNA methylation 2-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q8RWL49.3e-7142.82Increased DNA methylation 2 OS=Arabidopsis thaliana OX=3702 GN=IDM2 PE=1 SV=1[more]
Q9SYQ07.9e-5429.96Increased DNA methylation 3 OS=Arabidopsis thaliana OX=3702 GN=IDM3 PE=1 SV=1[more]
Q84K791.1e-5056.07Alpha-crystallin domain-containing protein 22.3 OS=Arabidopsis thaliana OX=3702 ... [more]
Match NameE-valueIdentityDescription
A0A6J1FC977.1e-19986.99increased DNA methylation 2-like OS=Cucurbita moschata OX=3662 GN=LOC111444304 P... [more]
A0A6J1HLA01.1e-19686.22increased DNA methylation 2-like OS=Cucurbita maxima OX=3661 GN=LOC111465577 PE=... [more]
A0A5A7TYA71.0e-19285.68Increased DNA methylation 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaf... [more]
A0A1S3BZJ41.0e-19285.68increased DNA methylation 2 OS=Cucumis melo OX=3656 GN=LOC103495173 PE=4 SV=1[more]
Q84KA71.7e-19285.68Heat-shock protein-like protein OS=Cucumis melo subsp. melo OX=412675 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G54840.16.6e-7242.82HSP20-like chaperones superfamily protein [more]
AT1G20870.15.6e-5529.96HSP20-like chaperones superfamily protein [more]
AT1G54850.17.6e-5256.07HSP20-like chaperones superfamily protein [more]
AT1G54840.21.1e-3736.46HSP20-like chaperones superfamily protein [more]
AT1G76440.15.5e-2650.00HSP20-like chaperones superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR008978HSP20-like chaperoneGENE3D2.60.40.790coord: 287..391
e-value: 1.7E-12
score: 49.2
IPR008978HSP20-like chaperoneSUPERFAMILY49764HSP20-like chaperonescoord: 273..375
NoneNo IPR availablePANTHERPTHR34661:SF3INCREASED DNA METHYLATION 2coord: 18..388
NoneNo IPR availableCDDcd06464ACD_sHsps-likecoord: 289..387
e-value: 4.00704E-14
score: 65.268
IPR039321Increased DNA methylation 2/3-likePANTHERPTHR34661INCREASED DNA METHYLATION 3coord: 18..388
IPR002068Alpha crystallin/Hsp20 domainPROSITEPS01031SHSPcoord: 276..391
score: 10.740755

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0006626.1Lag0006626.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034440 lipid oxidation
biological_process GO:1901537 positive regulation of DNA demethylation
cellular_component GO:0005634 nucleus
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen