Lag0006591 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0006591
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionLipoxygenase
Locationchr6: 44011552 .. 44013542 (+)
RNA-Seq ExpressionLag0006591
SyntenyLag0006591
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTTATAGGGAAAGTGATTTTATTAAGAACTAATCCTTTGGATGTGACTGAGATTAAGGGAAAAAACGTTTATATACGACTAATCAGCTCTGTTAATGGTGACCCATGTAAGTTTCTTTTCTTCTCTTCTTTAATTTAATTTATTTTATTTTTTTTAATATTACATCACATATACAAAGTGAGAAATTTACCATATGTTTAAATCATGTAAAAATATTGTTTTAACTCCTAGATTATGTTTAGAGGAAAATAAGAGTATGTCTAGTATATACAGTTCAGTTTTTGTTTATTTGTTTTTTAGATTCATCCAATTAAATGAATTTAGTGAATATTTGTTTCGTAAATTTTAATAGTTTATGTTTTTTCTTTTTCTTGGCAAACAACAATTTTATGTTGTTTAGATTCTAAATTTGAAATTTGAAAAAGGGAGAACTACAAAGTTGAATAAATTTTTTTCAAAAATAATTATAAAAAAATTGTAAAATGAAATACGATAAATTTATTTTATAAAATAGGTTAAATTTGTAAATCTTCCACCATACATGTTTGAAACATTGACAAATATAAAATACAACTTTGTTTTTCATTTGACTTCTTTATTTTCGAAATTATATACGTTTTTGGATCACTAACTAAAGCACCCTAAAGTTTCCATCTGACCTAAGTCTAATTCCTAATCTCGATTCCTCTTATGATTATTAATATTTAATGGATTATTGTTCAAAAATAATAAAAATATAAATGTGGAAATGGAAAGTATTTATTTTGGGAACACTATAATCTCTAAATTAAGGTTTCGTTTGGAATACCGTCTTTTAGTTTAAGTTTTTTTCAAGAATCCATTTTTTAAAAACATTTCGTATAAAAATTGTTTTAGAATTTGATCGCTCATTTGTTTGGTTACATTTCCATTCAAACTTTTTGTCTCATTTAATTTCAATTTTTTTTGTATGTGTTTTTATACAGACAATGAAATGCAAGGGAAGGTGGGAAAGAAAACTTACTTGGAAAAGGTTACAACTCAAAATAGATCTTTATCTCCAAGGGAAACTGTGTTGAATGTCACTTTTGATTGGGATAAAGAGATAGAAATCCCAGGAGCTTTCTTCATCAGAAACGATCATAAAACTAAATTTCACCTCAAGTCCCTCACTCTTGAAAACGTTCCTGGTGTTGGAAAGATTTACTTCAACTGTAACTCTTGGATTTATCCTTCTAAAAAGTACGATAAAGATCGCATTTTCTTTGCCAATAAGGTAACTAATTCTTCGATCAAATTTTCCTTTGTGACTGTTTTTTTTTTTTTAAGTTCAAAATTTGAGTAGTTAAATAAACAGTAAACTATGTATATTAGATAACATGAGGTTTCATCTAAAAACCAATTGGCGATGAGAGGAGTAACTCATACATCTTATAAATTGTGAGGTCTCCACTTATCTTTTTAATGTGGGATCCTCAACATACCCCCTCAAGATGGTTCCTTTTTGGGTTTACCATTCTTGGATCGGATCTTTTTTTTTTTTTTTTTTTACCGAATACTCGTTTTGGTTTCATGGACTCTGATACCATAAACAAAATAATCAAACCAACAAAATATATGCAAGATCAAACCCTAAAGAAGCGAAACAAAAGTATTTGAAGAACTTGAATTCAAATTAAAACTAGGGCTTGCTCTGAATAAGATTGAATGTTTCGTTCGATGAGAAGAGACAGAAGGCACACAGAAGAATTGACGTGTGGTGAGGAGAGAAAAAAAATCAGACACTAGTCAATGAGCTCTGATACCATATAGAAATTCAGAGTTTAATGATAATTCTCCTCATATATTTCATTAATAAGAAAAGAGTATTTATACACATGGAGGAGAATAAAAAAATAATAATCACTAAACTGAATAAATAACATAATAAAAAAAAACTAAACTTATATACATGATGGTAATTTGTAACAACTCAGGCCCAAGATTCGGAATTCGGATTCGGCACCTAA

mRNA sequence

ATGCTTATAGGGAAAGTGATTTTATTAAGAACTAATCCTTTGGATGTGACTGAGATTAAGGGAAAAAACGTTTATATACGACTAATCAGCTCTGTTAATGGTGACCCATACAATGAAATGCAAGGGAAGGTGGGAAAGAAAACTTACTTGGAAAAGGTTACAACTCAAAATAGATCTTTATCTCCAAGGGAAACTGTGTTGAATGTCACTTTTGATTGGGATAAAGAGATAGAAATCCCAGGAGCTTTCTTCATCAGAAACGATCATAAAACTAAATTTCACCTCAAGTCCCTCACTCTTGAAAACGTTCCTGGTGTTGGAAAGATTTACTTCAACTGTAACTCTTGGATTTATCCTTCTAAAAAGTACGATAAAGATCGCATTTTCTTTGCCAATAAGGCCCAAGATTCGGAATTCGGATTCGGCACCTAA

Coding sequence (CDS)

ATGCTTATAGGGAAAGTGATTTTATTAAGAACTAATCCTTTGGATGTGACTGAGATTAAGGGAAAAAACGTTTATATACGACTAATCAGCTCTGTTAATGGTGACCCATACAATGAAATGCAAGGGAAGGTGGGAAAGAAAACTTACTTGGAAAAGGTTACAACTCAAAATAGATCTTTATCTCCAAGGGAAACTGTGTTGAATGTCACTTTTGATTGGGATAAAGAGATAGAAATCCCAGGAGCTTTCTTCATCAGAAACGATCATAAAACTAAATTTCACCTCAAGTCCCTCACTCTTGAAAACGTTCCTGGTGTTGGAAAGATTTACTTCAACTGTAACTCTTGGATTTATCCTTCTAAAAAGTACGATAAAGATCGCATTTTCTTTGCCAATAAGGCCCAAGATTCGGAATTCGGATTCGGCACCTAA

Protein sequence

MLIGKVILLRTNPLDVTEIKGKNVYIRLISSVNGDPYNEMQGKVGKKTYLEKVTTQNRSLSPRETVLNVTFDWDKEIEIPGAFFIRNDHKTKFHLKSLTLENVPGVGKIYFNCNSWIYPSKKYDKDRIFFANKAQDSEFGFGT
Homology
BLAST of Lag0006591 vs. NCBI nr
Match: XP_022150808.1 (probable linoleate 9S-lipoxygenase 5 [Momordica charantia])

HSP 1 Score: 172.9 bits (437), Expect = 1.9e-39
Identity = 88/142 (61.97%), Postives = 104/142 (73.24%), Query Frame = 0

Query: 4   GKVILLRTNPLDVTEIK-----------GKNVYIRLISSVNGDPYNEMQGKVGKKTYLEK 63
           G VILLR+N LD  EI+           G  V ++LISSVNGD   E QGKVGKK YLE 
Sbjct: 21  GTVILLRSNVLDYNEIQSSVLDNISEFWGARVSLQLISSVNGDREKEFQGKVGKKAYLEN 80

Query: 64  VTTQNRSLSPRETVLNVTFDWDKEIEIPGAFFIRNDHKTKFHLKSLTLENVPGVGKIYFN 123
             T+   ++P+ET   VTFDWD+E+ IPGAFFIRN+H TKF LKSLTLE+VP VG+I+F+
Sbjct: 81  WITKMVPVNPKETTFKVTFDWDEEMGIPGAFFIRNNHFTKFFLKSLTLEDVPLVGRIHFD 140

Query: 124 CNSWIYPSKKYDKDRIFFANKA 135
           CNSWIYPS KY+KDRIFF NKA
Sbjct: 141 CNSWIYPSIKYNKDRIFFVNKA 162

BLAST of Lag0006591 vs. NCBI nr
Match: XP_038890793.1 (probable linoleate 9S-lipoxygenase 5 [Benincasa hispida])

HSP 1 Score: 167.5 bits (423), Expect = 8.1e-38
Identity = 85/142 (59.86%), Postives = 101/142 (71.13%), Query Frame = 0

Query: 4   GKVILLRTNPLDVTEIK-----------GKNVYIRLISSVNGDPYNEMQGKVGKKTYLEK 63
           GKV+LLR+N LD  EI+           G  V  +LISSVNGD   E+QGKVGKKTYL  
Sbjct: 22  GKVVLLRSNSLDYNEIQSSVLDNISEFWGAKVSFQLISSVNGDRSKELQGKVGKKTYLGN 81

Query: 64  VTTQNRSLSPRETVLNVTFDWDKEIEIPGAFFIRNDHKTKFHLKSLTLENVPGVGKIYFN 123
              +   +SP ETV  ++FDWD++I IPGAF IRN+H TKF LKSLTLE+VP VG+I+F+
Sbjct: 82  WMAKMVPVSPGETVFKISFDWDEDIGIPGAFLIRNNHFTKFFLKSLTLEDVPCVGRIHFD 141

Query: 124 CNSWIYPSKKYDKDRIFFANKA 135
           CNSWIYPS KY  DRIFF NKA
Sbjct: 142 CNSWIYPSGKYKSDRIFFVNKA 163

BLAST of Lag0006591 vs. NCBI nr
Match: XP_008454500.1 (PREDICTED: probable linoleate 9S-lipoxygenase 5 [Cucumis melo])

HSP 1 Score: 165.6 bits (418), Expect = 3.1e-37
Identity = 85/142 (59.86%), Postives = 101/142 (71.13%), Query Frame = 0

Query: 4   GKVILLRTNPLDVTEIK-----------GKNVYIRLISSVNGDPYNEMQGKVGKKTYLEK 63
           G+VILLR+N LD  EI+           G  V  +LISS NGD   E+QGKVGKKTYL  
Sbjct: 20  GQVILLRSNSLDYNEIQSSVLDNISEFWGAKVSFQLISSENGDRSKELQGKVGKKTYLGN 79

Query: 64  VTTQNRSLSPRETVLNVTFDWDKEIEIPGAFFIRNDHKTKFHLKSLTLENVPGVGKIYFN 123
             T+   +SP ETV  ++FDWD++I IPGAF IRN+H TKF LKSLTLE+VP VG+I+F+
Sbjct: 80  WMTKMVPVSPGETVFKISFDWDEDIGIPGAFLIRNNHFTKFFLKSLTLEDVPFVGRIHFD 139

Query: 124 CNSWIYPSKKYDKDRIFFANKA 135
           CNSWIYPS KY  DRIFF NKA
Sbjct: 140 CNSWIYPSGKYKNDRIFFINKA 161

BLAST of Lag0006591 vs. NCBI nr
Match: KAB2620825.1 (linoleate 9S-lipoxygenase 5 [Pyrus ussuriensis x Pyrus communis] >KAB2621466.1 linoleate 9S-lipoxygenase 5 [Pyrus ussuriensis x Pyrus communis])

HSP 1 Score: 164.1 bits (414), Expect = 9.0e-37
Identity = 77/141 (54.61%), Postives = 102/141 (72.34%), Query Frame = 0

Query: 4   GKVILLRTNPLDVTEIK-----------GKNVYIRLISSVNGDPYNEMQGKVGKKTYLEK 63
           G+V+L++   LDV ++K           GK V +RLISSVNGDP NE++GKVGK  YLE 
Sbjct: 28  GRVVLMKKVVLDVNDLKASILDRVDELLGKAVSLRLISSVNGDPENELKGKVGKPAYLEN 87

Query: 64  VTTQNRSLSPRETVLNVTFDWDKEIEIPGAFFIRNDHKTKFHLKSLTLENVPGVGKIYFN 123
             T    L+  E   +VTFDWDKEI IPGAF +RN+H ++F+LK+LTLE+VPG G+++F 
Sbjct: 88  WVTTITPLTAGEAAFDVTFDWDKEIGIPGAFVVRNEHHSEFYLKTLTLEDVPGEGRVHFV 147

Query: 124 CNSWIYPSKKYDKDRIFFANK 134
           CNSW+YP+ KY KDR+FF+NK
Sbjct: 148 CNSWVYPADKYKKDRVFFSNK 168

BLAST of Lag0006591 vs. NCBI nr
Match: XP_009376663.1 (PREDICTED: probable linoleate 9S-lipoxygenase 5 [Pyrus x bretschneideri] >XP_009376684.1 PREDICTED: probable linoleate 9S-lipoxygenase 5 [Pyrus x bretschneideri])

HSP 1 Score: 163.7 bits (413), Expect = 1.2e-36
Identity = 76/141 (53.90%), Postives = 102/141 (72.34%), Query Frame = 0

Query: 4   GKVILLRTNPLDVTEIK-----------GKNVYIRLISSVNGDPYNEMQGKVGKKTYLEK 63
           G+V+L++   LDV ++K           GK V +RLISSVNGDP NE++GKVGK  YLE 
Sbjct: 28  GRVVLMKKVVLDVNDLKASILDRVDELLGKAVSLRLISSVNGDPENELKGKVGKPAYLEN 87

Query: 64  VTTQNRSLSPRETVLNVTFDWDKEIEIPGAFFIRNDHKTKFHLKSLTLENVPGVGKIYFN 123
             T    L+  E   +VTFDWDKEI +PGAF +RN+H ++F+LK+LTLE+VPG G+++F 
Sbjct: 88  WVTTITPLTAGEAAFDVTFDWDKEIGVPGAFVVRNEHHSEFYLKTLTLEDVPGEGRVHFV 147

Query: 124 CNSWIYPSKKYDKDRIFFANK 134
           CNSW+YP+ KY KDR+FF+NK
Sbjct: 148 CNSWVYPADKYKKDRVFFSNK 168

BLAST of Lag0006591 vs. ExPASy Swiss-Prot
Match: Q43191 (Probable linoleate 9S-lipoxygenase 5 OS=Solanum tuberosum OX=4113 GN=LOX1.5 PE=2 SV=1)

HSP 1 Score: 142.9 bits (359), Expect = 2.8e-33
Identity = 70/142 (49.30%), Postives = 92/142 (64.79%), Query Frame = 0

Query: 4   GKVILLRTNPLD-----------VTEIKGKNVYIRLISSVNGDPYNEMQGKVGKKTYLEK 63
           G ++L++ N LD           V E  GK V ++LIS V+ DP N +QGK     YLEK
Sbjct: 23  GTIVLMKKNVLDFNDVNASLLDGVLEFLGKRVSLQLISVVHADPGNSLQGKRSNPAYLEK 82

Query: 64  VTTQNRSLSPRETVLNVTFDWDKEIEIPGAFFIRNDHKTKFHLKSLTLENVPGVGKIYFN 123
             T   SL   E+  +VTFDWD++I +PGAF I N H  +F+LKSLTLE+VP  G ++F 
Sbjct: 83  WLTTGTSLVAGESAFDVTFDWDEDIGVPGAFIINNFHFNEFYLKSLTLEDVPNHGNVHFV 142

Query: 124 CNSWIYPSKKYDKDRIFFANKA 135
           CNSW+YP+KKY  +RIFFAN+A
Sbjct: 143 CNSWVYPAKKYKSERIFFANQA 164

BLAST of Lag0006591 vs. ExPASy Swiss-Prot
Match: Q43190 (Probable linoleate 9S-lipoxygenase 4 OS=Solanum tuberosum OX=4113 GN=LOX1.4 PE=2 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 7.7e-31
Identity = 66/141 (46.81%), Postives = 87/141 (61.70%), Query Frame = 0

Query: 4   GKVILLRTNPLDVTEIK-----------GKNVYIRLISSVNGDPYNEMQGKVGKKTYLEK 63
           G V++++ N LD T++            G+ V  +LISSV  DP N +QGK     YLE 
Sbjct: 21  GTVVMMKKNALDFTDLAGSLTDKIFEALGQKVSFQLISSVQSDPANGLQGKHSNPAYLEN 80

Query: 64  VTTQNRSLSPRETVLNVTFDWDKEIEIPGAFFIRNDHKTKFHLKSLTLENVPGVGKIYFN 123
                  L+  ET   VTFDW++E  +PGAF I+N H  +F LKSLTLE+VP  GK++F 
Sbjct: 81  FLFTLTPLAAGETAFGVTFDWNEEFGVPGAFIIKNTHINEFFLKSLTLEDVPNHGKVHFV 140

Query: 124 CNSWIYPSKKYDKDRIFFANK 134
           CNSW+YPS +Y  DRIFFAN+
Sbjct: 141 CNSWVYPSFRYKSDRIFFANQ 161

BLAST of Lag0006591 vs. ExPASy Swiss-Prot
Match: Q41238 (Linoleate 9S-lipoxygenase 6 (Fragment) OS=Solanum tuberosum OX=4113 GN=LOX1.6 PE=1 SV=1)

HSP 1 Score: 134.8 bits (338), Expect = 7.7e-31
Identity = 66/141 (46.81%), Postives = 87/141 (61.70%), Query Frame = 0

Query: 4   GKVILLRTNPLDVTEIK-----------GKNVYIRLISSVNGDPYNEMQGKVGKKTYLEK 63
           G V++++ N LD T++            G+ V  +LISSV  DP N +QGK     YLE 
Sbjct: 18  GTVVMMKKNALDFTDLAGSLTDKIFEALGQKVSFQLISSVQSDPANGLQGKHSNPAYLEN 77

Query: 64  VTTQNRSLSPRETVLNVTFDWDKEIEIPGAFFIRNDHKTKFHLKSLTLENVPGVGKIYFN 123
                  L+  ET   VTFDW++E  +PGAF I+N H  +F LKSLTLE+VP  GK++F 
Sbjct: 78  FLFTLTPLAAGETAFGVTFDWNEEFGVPGAFIIKNTHINEFFLKSLTLEDVPNHGKVHFV 137

Query: 124 CNSWIYPSKKYDKDRIFFANK 134
           CNSW+YPS +Y  DRIFFAN+
Sbjct: 138 CNSWVYPSFRYKSDRIFFANQ 158

BLAST of Lag0006591 vs. ExPASy Swiss-Prot
Match: P38415 (Linoleate 9S-lipoxygenase A OS=Solanum lycopersicum OX=4081 GN=LOX1.1 PE=2 SV=1)

HSP 1 Score: 134.0 bits (336), Expect = 1.3e-30
Identity = 66/141 (46.81%), Postives = 87/141 (61.70%), Query Frame = 0

Query: 4   GKVILLRTNPLDVTEIK-----------GKNVYIRLISSVNGDPYNEMQGKVGKKTYLEK 63
           G V++++ N LD T++            G+ V  +LISSV  DP N +QGK     YLE 
Sbjct: 21  GTVVMMKKNALDFTDLAGSLTDKIFEALGQKVSFQLISSVQSDPANGLQGKHSNPAYLEN 80

Query: 64  VTTQNRSLSPRETVLNVTFDWDKEIEIPGAFFIRNDHKTKFHLKSLTLENVPGVGKIYFN 123
                  L+  ET   VTFDW++E  +PGAF I+N H  +F LKSLTLE+VP  GK++F 
Sbjct: 81  FLLTLTPLAAGETAFGVTFDWNEEFGVPGAFVIKNMHINEFFLKSLTLEDVPNHGKVHFV 140

Query: 124 CNSWIYPSKKYDKDRIFFANK 134
           CNSW+YPS +Y  DRIFFAN+
Sbjct: 141 CNSWVYPSFRYKSDRIFFANQ 161

BLAST of Lag0006591 vs. ExPASy Swiss-Prot
Match: Q43189 (Probable linoleate 9S-lipoxygenase 3 OS=Solanum tuberosum OX=4113 GN=LOX1.3 PE=2 SV=1)

HSP 1 Score: 133.3 bits (334), Expect = 2.2e-30
Identity = 68/142 (47.89%), Postives = 88/142 (61.97%), Query Frame = 0

Query: 4   GKVILLRTNPLDVT-----------EIKGKNVYIRLISSVNGDPYNEMQGKVGKKTYLE- 63
           G V+++  N LD T           ++ G+ V  +LISSV GDP N +QGK     YLE 
Sbjct: 21  GTVVMMNKNVLDFTDLASSLTGKIFDVLGQKVSFQLISSVQGDPTNGLQGKHSNPAYLEN 80

Query: 64  KVTTQNRSLSPRETVLNVTFDWDKEIEIPGAFFIRNDHKTKFHLKSLTLENVPGVGKIYF 123
            + T     +  ET   VTFDW++E  +PGAF I+N H T+F LKSLTLE+VP  GK++F
Sbjct: 81  SLFTLTPLTAGSETAFGVTFDWNEEFGVPGAFIIKNMHITEFFLKSLTLEDVPNHGKVHF 140

Query: 124 NCNSWIYPSKKYDKDRIFFANK 134
            CNSW+YPS  Y  DRIFFAN+
Sbjct: 141 VCNSWVYPSLNYKSDRIFFANQ 162

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022150808.11.9e-3961.97probable linoleate 9S-lipoxygenase 5 [Momordica charantia][more]
XP_038890793.18.1e-3859.86probable linoleate 9S-lipoxygenase 5 [Benincasa hispida][more]
XP_008454500.13.1e-3759.86PREDICTED: probable linoleate 9S-lipoxygenase 5 [Cucumis melo][more]
KAB2620825.19.0e-3754.61linoleate 9S-lipoxygenase 5 [Pyrus ussuriensis x Pyrus communis] >KAB2621466.1 l... [more]
XP_009376663.11.2e-3653.90PREDICTED: probable linoleate 9S-lipoxygenase 5 [Pyrus x bretschneideri] >XP_009... [more]
Match NameE-valueIdentityDescription
Q431912.8e-3349.30Probable linoleate 9S-lipoxygenase 5 OS=Solanum tuberosum OX=4113 GN=LOX1.5 PE=2... [more]
Q431907.7e-3146.81Probable linoleate 9S-lipoxygenase 4 OS=Solanum tuberosum OX=4113 GN=LOX1.4 PE=2... [more]
Q412387.7e-3146.81Linoleate 9S-lipoxygenase 6 (Fragment) OS=Solanum tuberosum OX=4113 GN=LOX1.6 PE... [more]
P384151.3e-3046.81Linoleate 9S-lipoxygenase A OS=Solanum lycopersicum OX=4081 GN=LOX1.1 PE=2 SV=1[more]
Q431892.2e-3047.89Probable linoleate 9S-lipoxygenase 3 OS=Solanum tuberosum OX=4113 GN=LOX1.3 PE=2... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001024PLAT/LH2 domainSMARTSM00308LH2_4coord: 4..132
e-value: 1.3E-30
score: 117.7
IPR001024PLAT/LH2 domainPFAMPF01477PLATcoord: 31..130
e-value: 5.4E-16
score: 59.2
IPR001024PLAT/LH2 domainPROSITEPS50095PLATcoord: 1..131
score: 17.683865
NoneNo IPR availableGENE3D2.60.60.20PLAT/LH2 domaincoord: 2..134
e-value: 1.6E-43
score: 150.5
NoneNo IPR availablePANTHERPTHR11771:SF126LINOLEATE 9S-LIPOXYGENASE 1coord: 16..134
IPR000907LipoxygenasePANTHERPTHR11771LIPOXYGENASEcoord: 16..134
IPR036392PLAT/LH2 domain superfamilySUPERFAMILY49723Lipase/lipooxygenase domain (PLAT/LH2 domain)coord: 15..133

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0006591.1Lag0006591.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046394 carboxylic acid biosynthetic process
biological_process GO:0006631 fatty acid metabolic process
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen
molecular_function GO:0005515 protein binding