Lag0006558 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0006558
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	protein RALF-like 33
Location	chr6: 43593961 .. 43594326 (-)
RNA-Seq Expression	Lag0006558
Synteny	Lag0006558

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCAATGGCCGGATTCAATTCTCCGACCTTCTTCCTGATCTGCGCCGCCGTGTTTGTGGTCTTCAGCTGCTCCTCAACCGCCGTCCACGGCGGCCTCGGGATCCACCACAGCCTCGCATGGATTCCTCACCAATCGACCTGCGAGGGCTCCATAGCCGAGTGTTTCGGCGGAGAGGAGTTCGAATTCGATTCGGAAATCAGCCGCCGCATCTTGGCCACCAGCCAGTACATCAGCTATGGCGCTCTGAGGAGGAACACGGTGCCGTGTTCTAGACGCGGTGCATCCTACTACAATTGCCAGCCCGGAGCTCAGGCCAATCCGTACAGCCGTGGATGCAGCGCCATTACGAGGTGCAGGAGTTGA

mRNA sequence

ATGGCAATGGCCGGATTCAATTCTCCGACCTTCTTCCTGATCTGCGCCGCCGTGTTTGTGGTCTTCAGCTGCTCCTCAACCGCCGTCCACGGCGGCCTCGGGATCCACCACAGCCTCGCATGGATTCCTCACCAATCGACCTGCGAGGGCTCCATAGCCGAGTGTTTCGGCGGAGAGGAGTTCGAATTCGATTCGGAAATCAGCCGCCGCATCTTGGCCACCAGCCAGTACATCAGCTATGGCGCTCTGAGGAGGAACACGGTGCCGTGTTCTAGACGCGGTGCATCCTACTACAATTGCCAGCCCGGAGCTCAGGCCAATCCGTACAGCCGTGGATGCAGCGCCATTACGAGGTGCAGGAGTTGA

Coding sequence (CDS)

ATGGCAATGGCCGGATTCAATTCTCCGACCTTCTTCCTGATCTGCGCCGCCGTGTTTGTGGTCTTCAGCTGCTCCTCAACCGCCGTCCACGGCGGCCTCGGGATCCACCACAGCCTCGCATGGATTCCTCACCAATCGACCTGCGAGGGCTCCATAGCCGAGTGTTTCGGCGGAGAGGAGTTCGAATTCGATTCGGAAATCAGCCGCCGCATCTTGGCCACCAGCCAGTACATCAGCTATGGCGCTCTGAGGAGGAACACGGTGCCGTGTTCTAGACGCGGTGCATCCTACTACAATTGCCAGCCCGGAGCTCAGGCCAATCCGTACAGCCGTGGATGCAGCGCCATTACGAGGTGCAGGAGTTGA

Protein sequence

MAMAGFNSPTFFLICAAVFVVFSCSSTAVHGGLGIHHSLAWIPHQSTCEGSIAECFGGEEFEFDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITRCRS

Homology

[Close]

BLAST of Lag0006558 vs. NCBI nr
Match: XP_038889183.1 (protein RALF-like 33 [Benincasa hispida])

HSP 1 Score: 243.8 bits (621), Expect = 7.6e-61
Identity = 116/121 (95.87%), Postives = 120/121 (99.17%), Query Frame = 0


Query: 1   MAMAGFNSPTFFLICAAVFVVFSCSSTAVHGGLGIHHSLAWIPHQSTCEGSIAECFGGEE 60
           MAMAGFNSPTFFLICAAVFV+FSCSSTAVH GLGIHHSLAWIP+QSTC+GSIAECFGGEE
Sbjct: 1   MAMAGFNSPTFFLICAAVFVIFSCSSTAVHAGLGIHHSLAWIPNQSTCKGSIAECFGGEE 60

Query: 61  FEFDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITRCR 120
           FEFDSEI+RRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITRCR
Sbjct: 61  FEFDSEINRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITRCR 120

Query: 121 S 122
           S
Sbjct: 121 S 121

[Close]

BLAST of Lag0006558 vs. NCBI nr
Match: XP_022966015.1 (protein RALF-like 33 [Cucurbita maxima] >XP_023537429.1 protein RALF-like 33 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 241.9 bits (616), Expect = 2.9e-60
Identity = 116/121 (95.87%), Postives = 119/121 (98.35%), Query Frame = 0


Query: 1   MAMAGFNSPTFFLICAAVFVVFSCSSTAVHGGLGIHHSLAWIPHQSTCEGSIAECFGGEE 60
           MAMAGFNS TFFLICAAVFVVFSCSSTAVHGGLGIHHSL WIP+QSTC+G+IAECFGGEE
Sbjct: 1   MAMAGFNSSTFFLICAAVFVVFSCSSTAVHGGLGIHHSLGWIPNQSTCKGTIAECFGGEE 60

Query: 61  FEFDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITRCR 120
           FEFDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITRCR
Sbjct: 61  FEFDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITRCR 120

Query: 121 S 122
           S
Sbjct: 121 S 121

[Close]

BLAST of Lag0006558 vs. NCBI nr
Match: XP_022937540.1 (protein RALF-like 33 [Cucurbita moschata])

HSP 1 Score: 238.8 bits (608), Expect = 2.4e-59
Identity = 115/121 (95.04%), Postives = 118/121 (97.52%), Query Frame = 0


Query: 1   MAMAGFNSPTFFLICAAVFVVFSCSSTAVHGGLGIHHSLAWIPHQSTCEGSIAECFGGEE 60
           MAMAGFNS TFFLICAAVFVVFSCSSTAVHGGLGI HSL WIP+QSTC+G+IAECFGGEE
Sbjct: 1   MAMAGFNSSTFFLICAAVFVVFSCSSTAVHGGLGIQHSLGWIPNQSTCKGTIAECFGGEE 60

Query: 61  FEFDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITRCR 120
           FEFDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITRCR
Sbjct: 61  FEFDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITRCR 120

Query: 121 S 122
           S
Sbjct: 121 S 121

[Close]

BLAST of Lag0006558 vs. NCBI nr
Match: KAG6586247.1 (Protein RALF-like 33, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 235.3 bits (599), Expect = 2.7e-58
Identity = 113/119 (94.96%), Postives = 116/119 (97.48%), Query Frame = 0


Query: 3   MAGFNSPTFFLICAAVFVVFSCSSTAVHGGLGIHHSLAWIPHQSTCEGSIAECFGGEEFE 62
           MAGFNS TFFLICAAVFVVFSCSSTAVHGGLGI HSL WIP+QSTC+G+IAECFGGEEFE
Sbjct: 1   MAGFNSSTFFLICAAVFVVFSCSSTAVHGGLGIQHSLGWIPNQSTCKGTIAECFGGEEFE 60

Query: 63  FDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITRCRS 122
           FDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITRCRS
Sbjct: 61  FDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITRCRS 119

[Close]

BLAST of Lag0006558 vs. NCBI nr
Match: XP_022150733.1 (protein RALF-like 33 [Momordica charantia])

HSP 1 Score: 233.0 bits (593), Expect = 1.3e-57
Identity = 113/121 (93.39%), Postives = 118/121 (97.52%), Query Frame = 0


Query: 1   MAMAGFNSPTFFLICAAVFVVFSCSSTAVHGGLGIHHSLAWIPHQSTCEGSIAECFGGEE 60
           MAMAGF+SPT FLICAAVFVVF+CSSTAVHGGLGIHHSLAWIP QS+C+GSIAECFGGEE
Sbjct: 1   MAMAGFHSPT-FLICAAVFVVFTCSSTAVHGGLGIHHSLAWIPDQSSCKGSIAECFGGEE 60

Query: 61  FEFDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITRCR 120
           FEFDSEISRRILATSQYISYGAL+RNTVPCSRRGASYYNCQPGAQANPY RGCSAITRCR
Sbjct: 61  FEFDSEISRRILATSQYISYGALKRNTVPCSRRGASYYNCQPGAQANPYGRGCSAITRCR 120

Query: 121 S 122
           S
Sbjct: 121 S 120

[Close]

BLAST of Lag0006558 vs. ExPASy Swiss-Prot
Match: Q8L9P8 (Protein RALF-like 33 OS=Arabidopsis thaliana OX=3702 GN=RALFL33 PE=2 SV=1)

HSP 1 Score: 132.9 bits (333), Expect = 2.5e-30
Identity = 64/83 (77.11%), Postives = 72/83 (86.75%), Query Frame = 0


Query: 41  WIPHQSTCEGSIAEC---FGGEEFEFDSEISRRILATSQYISYGALRRNTVPCSRRGASY 100
           ++P +S C G+IAEC      EEFE DSEI+RRILAT++YISYGALRRNTVPCSRRGASY
Sbjct: 33  FVPIESKCNGTIAECSLSTAEEEFEMDSEINRRILATTKYISYGALRRNTVPCSRRGASY 92

Query: 101 YNCQPGAQANPYSRGCSAITRCR 121
           YNC+ GAQANPYSRGCSAITRCR
Sbjct: 93  YNCRRGAQANPYSRGCSAITRCR 115

[Close]

BLAST of Lag0006558 vs. ExPASy Swiss-Prot
Match: Q9SRY3 (Protein RALF-like 1 OS=Arabidopsis thaliana OX=3702 GN=RALF1 PE=1 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 2.1e-29
Identity = 72/116 (62.07%), Postives = 81/116 (69.83%), Query Frame = 0


Query: 10  TFFLICAAVFVVFSCSSTAVHGGLGIH-HSLAWI---PHQSTCEGSIAECFGGEEFEFDS 69
           T FL    + VVF  SS  V  G       +AW     + S C GSIAEC G EE E DS
Sbjct: 6   TLFL-TLTILVVFIISSPPVQAGFANDLGGVAWATTGDNGSGCHGSIAECIGAEEEEMDS 65

Query: 70  EISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITRCRS 122
           EI+RRILAT++YISY +L+RN+VPCSRRGASYYNCQ GAQANPYSRGCS I RCRS
Sbjct: 66  EINRRILATTKYISYQSLKRNSVPCSRRGASYYNCQNGAQANPYSRGCSKIARCRS 120

[Close]

BLAST of Lag0006558 vs. ExPASy Swiss-Prot
Match: Q945T0 (Rapid alkalinization factor OS=Nicotiana tabacum OX=4097 GN=RALF PE=1 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 1.4e-28
Identity = 67/114 (58.77%), Postives = 80/114 (70.18%), Query Frame = 0


Query: 9   PTFFLICAAVFVVFSCSSTAVHGGLGIHHSLAWIPHQSTCEGSIAECFG-GEEFEFDSEI 68
           P+  ++C  +   F   S A  G  G +  +        C+GSI EC    EEFE DSE 
Sbjct: 4   PSGLILCVLIGAFF--ISMAAAGDSGAYDWVMPARSGGGCKGSIGECIAEEEEFELDSES 63

Query: 69  SRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGAQANPYSRGCSAITRCRS 122
           +RRILAT +YISYGAL++N+VPCSRRGASYYNC+PGAQANPYSRGCSAITRCRS
Sbjct: 64  NRRILATKKYISYGALQKNSVPCSRRGASYYNCKPGAQANPYSRGCSAITRCRS 115

[Close]

BLAST of Lag0006558 vs. ExPASy Swiss-Prot
Match: Q9LUS7 (Rapid alkalinization factor 23 OS=Arabidopsis thaliana OX=3702 GN=RALF23 PE=1 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 1.4e-28
Identity = 64/95 (67.37%), Postives = 72/95 (75.79%), Query Frame = 0


Query: 43  PHQSTCEGSIAEC-------------FG----GEEFEFDSEISRRILATSQYISYGALRR 102
           P ++ C G+IAEC             +G    GEEFE DSEI+RRILAT +YISYGALRR
Sbjct: 42  PFETECRGTIAECSVSAALGDGGDLFYGGGEMGEEFEMDSEINRRILATRRYISYGALRR 101

Query: 103 NTVPCSRRGASYYNCQPGAQANPYSRGCSAITRCR 121
           NT+PCSRRGASYYNC+ GAQANPYSRGCSAITRCR
Sbjct: 102 NTIPCSRRGASYYNCRRGAQANPYSRGCSAITRCR 136

[Close]

BLAST of Lag0006558 vs. ExPASy Swiss-Prot
Match: Q9MA62 (Protein RALF-like 22 OS=Arabidopsis thaliana OX=3702 GN=RALFL22 PE=3 SV=1)

HSP 1 Score: 125.6 bits (314), Expect = 3.9e-28
Identity = 61/76 (80.26%), Postives = 65/76 (85.53%), Query Frame = 0


Query: 46  STCEGSIAECFG-GEEFEFDSEISRRILATSQYISYGALRRNTVPCSRRGASYYNCQPGA 105
           S C GSIAEC    EE EFDS+ISRRILA  +YISYGA+RRN+VPCSRRGASYYNCQ GA
Sbjct: 43  SGCTGSIAECIAEEEEMEFDSDISRRILAQKKYISYGAMRRNSVPCSRRGASYYNCQRGA 102

Query: 106 QANPYSRGCSAITRCR 121
           QANPYSRGCS ITRCR
Sbjct: 103 QANPYSRGCSTITRCR 118

The following BLAST results are available for this feature:

BLAST of Lag0006558 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038889183.1	7.6e-61	95.87	protein RALF-like 33 [Benincasa hispida]	[more]
XP_022966015.1	2.9e-60	95.87	protein RALF-like 33 [Cucurbita maxima] >XP_023537429.1 protein RALF-like 33 [Cu...	[more]
XP_022937540.1	2.4e-59	95.04	protein RALF-like 33 [Cucurbita moschata]	[more]
KAG6586247.1	2.7e-58	94.96	Protein RALF-like 33, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022150733.1	1.3e-57	93.39	protein RALF-like 33 [Momordica charantia]	[more]

BLAST of Lag0006558 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8L9P8	2.5e-30	77.11	Protein RALF-like 33 OS=Arabidopsis thaliana OX=3702 GN=RALFL33 PE=2 SV=1	[more]
Q9SRY3	2.1e-29	62.07	Protein RALF-like 1 OS=Arabidopsis thaliana OX=3702 GN=RALF1 PE=1 SV=1	[more]
Q945T0	1.4e-28	58.77	Rapid alkalinization factor OS=Nicotiana tabacum OX=4097 GN=RALF PE=1 SV=1	[more]
Q9LUS7	1.4e-28	67.37	Rapid alkalinization factor 23 OS=Arabidopsis thaliana OX=3702 GN=RALF23 PE=1 SV...	[more]
Q9MA62	3.9e-28	80.26	Protein RALF-like 22 OS=Arabidopsis thaliana OX=3702 GN=RALFL22 PE=3 SV=1	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR008801	Rapid ALkalinization Factor	PFAM	PF05498	RALF	coord: 59..120 e-value: 9.0E-31 score: 106.6
None	No IPR available	PANTHER	PTHR33136:SF50	PROTEIN RALF-LIKE 33	coord: 6..121
None	No IPR available	PANTHER	PTHR33136	RAPID ALKALINIZATION FACTOR-LIKE	coord: 6..121

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0006558.1	Lag0006558.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0019722	calcium-mediated signaling
cellular_component	GO:0005576	extracellular region