Lag0006549 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0006549 vs. NCBI nr
Match: XP_038877043.1 (LOW QUALITY PROTEIN: GDSL esterase/lipase At5g03980-like, partial [Benincasa hispida])

HSP 1 Score: 624.4 bits (1609), Expect = 6.3e-175
Identity = 294/367 (80.11%), Postives = 322/367 (87.74%), Query Frame = 0

Query: 1   MAASTTPLLTLLFLLLLSSSSSDAHSLKACKFDAIYQLGDSISDTGNLIRENPNTPFSHL 60
           MAAST  L   +FL L  SSS +AH LK C FDAIYQLGDSISDTGNLIREN NTPFSHL
Sbjct: 1   MAASTKSLWISIFLFLFLSSSFNAHLLKVCMFDAIYQLGDSISDTGNLIRENLNTPFSHL 60

Query: 61  PYGETFFKNSTGRCSNGLLMIDYFAQDIGIPLVNPYLNKDALTRHGVNFAVAGSTALSSE 120
           PYGE+FF N TGRCSNGLLMIDYFA D G+PLV+PYLNKDALT HGVNFAVAGSTALSSE
Sbjct: 61  PYGESFFNNPTGRCSNGLLMIDYFALDAGLPLVSPYLNKDALTNHGVNFAVAGSTALSSE 120

Query: 121 VLLEKKISAPKTNSPLDHQLDWMFSHFNSICYNQKDCNEKLKNALFLVGEIGANDYNYAF 180
           +L +KKIS+P TNS L HQLDWMFSHFNSICYNQ+DCNEKL NALFLVGEIG NDYNYA 
Sbjct: 121 LLFQKKISSPVTNSSLSHQLDWMFSHFNSICYNQRDCNEKLYNALFLVGEIGGNDYNYAL 180

Query: 181 FQGKTIEEVKDMVPQVVQTIKSGVERVISYGAAQVVVPGNFPIGCLPVYLTRFQTSNTTA 240
           FQGKTI+EVKDMVP VVQ+IKS VERVISYGA +VVVPGNFPIGC P+YLT FQT++T+A
Sbjct: 181 FQGKTIQEVKDMVPDVVQSIKSAVERVISYGATRVVVPGNFPIGCFPIYLTAFQTNDTSA 240

Query: 241 YDELHCLKSFNNLASYHNDQIKQAIEVLKKENPHIVIVYGDYYNAFLWILRHASVLGFDE 300
           YDELHCLK  N  ASYHNDQ+K+AIEVLKKENP  VIVYGDYYNAFLWILRHA VLGFDE
Sbjct: 241 YDELHCLKDLNGFASYHNDQVKEAIEVLKKENPXTVIVYGDYYNAFLWILRHAFVLGFDE 300

Query: 301 VSLQKSCCGIGGDYNFNHMKMCGLPGVPVCPNPDKYISWDGIHLTQMAYKSMAHWLIDDV 360
            SLQKSCCG+GG+YNFN MKMCGLPGV VC NP+++ISWDG+HLTQ AYK MAHWLI  +
Sbjct: 301 GSLQKSCCGVGGNYNFNLMKMCGLPGVEVCQNPNEHISWDGVHLTQKAYKFMAHWLIHAI 360

Query: 361 YPQLHCI 368
           +P+LHC+
Sbjct: 361 FPELHCL 367

BLAST of Lag0006549 vs. NCBI nr
Match: XP_022156428.1 (acetylajmalan esterase-like [Momordica charantia])

HSP 1 Score: 614.8 bits (1584), Expect = 5.0e-172
Identity = 291/365 (79.73%), Postives = 322/365 (88.22%), Query Frame = 0

Query: 3   ASTTPLLTLLFLLLLSSSSSDAHSLKACKFDAIYQLGDSISDTGNLIRENPNTPFSHLPY 62
           +S + LL LLFLLL  SS+S AH L+AC FDAIYQLGDSISDTGNLIRENPNT FSHLPY
Sbjct: 8   SSFSLLLPLLFLLL--SSASTAHPLRACMFDAIYQLGDSISDTGNLIRENPNTTFSHLPY 67

Query: 63  GETFFKNSTGRCSNGLLMIDYFAQDIGIPLVNPYLNKDALTRHGVNFAVAGSTALSSEVL 122
           GETFF  +TGRCSNGLLM+DYFA D G+PLVNPYLN+DALT HGVNFAVAGSTALS E L
Sbjct: 68  GETFFHGATGRCSNGLLMLDYFALDAGLPLVNPYLNRDALTNHGVNFAVAGSTALSFEAL 127

Query: 123 LEKKISAPKTNSPLDHQLDWMFSHFNSICYNQKDCNEKLKNALFLVGEIGANDYNYAFFQ 182
           L  KI +P TNS LDHQL WM SHF SIC N++DC EKL++ALFLVGEIG NDYNYA FQ
Sbjct: 128 LRNKILSPVTNSSLDHQLGWMHSHFESICLNERDCIEKLRSALFLVGEIGGNDYNYALFQ 187

Query: 183 GKTIEEVKDMVPQVVQTIKSGVERVISYGAAQVVVPGNFPIGCLPVYLTRFQTSNTTAYD 242
           GKTI+EVKDMVP VVQTIK+ VE+VISYGA +V+VPGNFPIGCLP+YLT FQT++TTAYD
Sbjct: 188 GKTIQEVKDMVPDVVQTIKNAVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTNDTTAYD 247

Query: 243 ELHCLKSFNNLASYHNDQIKQAIEVLKKENPHIVIVYGDYYNAFLWILRHASVLGFDEVS 302
           ELHCLK  N+LASYHNDQIKQAIEVLK+ENPH VIVYGDYYNA LWILRHA VLGFDE S
Sbjct: 248 ELHCLKGLNSLASYHNDQIKQAIEVLKRENPHTVIVYGDYYNALLWILRHAFVLGFDEAS 307

Query: 303 LQKSCCGIGGDYNFNHMKMCGLPGVPVCPNPDKYISWDGIHLTQMAYKSMAHWLIDDVYP 362
           LQKSCCGIGG+YNFN MK+CGLPGVPVCPNP ++ISWDGIHLTQ AYK MA+WLI D++P
Sbjct: 308 LQKSCCGIGGNYNFNLMKICGLPGVPVCPNPHEHISWDGIHLTQKAYKFMAYWLIHDIFP 367

Query: 363 QLHCI 368
           +LHCI
Sbjct: 368 KLHCI 370

BLAST of Lag0006549 vs. NCBI nr
Match: XP_023546438.1 (acetylajmalan esterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 609.8 bits (1571), Expect = 1.6e-170
Identity = 289/368 (78.53%), Postives = 323/368 (87.77%), Query Frame = 0

Query: 3   ASTTPLLTLLFLLL--LSSSSSDAHSLKACKFDAIYQLGDSISDTGNLIRENPNTPFSHL 62
           +S++ +L LL +LL  L   SS AH LK C FDAIYQLGDSISDTGNLIR+NPNTPFS+L
Sbjct: 7   SSSSIVLALLLVLLSVLGCPSSKAHLLKTCMFDAIYQLGDSISDTGNLIRQNPNTPFSNL 66

Query: 63  PYGETFFKNSTGRCSNGLLMIDYFAQDIGIPLVNPYLNKDALTRHGVNFAVAGSTALSSE 122
           PYGETFF  STGRCSNGLLMIDYFA D G+PLVNPYLNKDALTRHGVNFAVAGSTALSS+
Sbjct: 67  PYGETFFNKSTGRCSNGLLMIDYFALDAGLPLVNPYLNKDALTRHGVNFAVAGSTALSSQ 126

Query: 123 VLLEKKISAPKTNSPLDHQLDWMFSHFNSICYNQKDCNEKLKNALFLVGEIGANDYNYAF 182
           +L + +I +P TNS L+ QL WMFSHFNSICYNQ+DCN+KL+NALFLVGEIG NDYNYA 
Sbjct: 127 LLSQNQILSPLTNSSLNQQLYWMFSHFNSICYNQRDCNQKLRNALFLVGEIGGNDYNYAL 186

Query: 183 FQGKTIEEVKDMVPQVVQTIKSGVERVISYGAAQVVVPGNFPIGCLPVYLTRFQTSNTTA 242
           FQGKTI+EVKDMVPQVVQTIK+ VE+VISYGA +VVVPGNFPIGCLP+YLT FQT++TTA
Sbjct: 187 FQGKTIQEVKDMVPQVVQTIKNAVEKVISYGATRVVVPGNFPIGCLPIYLTGFQTNDTTA 246

Query: 243 YDELHCLKSFNNLASYHNDQIKQAIEVLKKENPHIVIVYGDYYNAFLWILRHASVLGFDE 302
           YDELHCLK  N LA+YHNDQIKQAIEVLK+ENPH +IVYGDYYNA LWILR A  LGFDE
Sbjct: 247 YDELHCLKDLNGLATYHNDQIKQAIEVLKRENPHTIIVYGDYYNALLWILRRAFTLGFDE 306

Query: 303 VSLQKSCCGIGGDYNFNHMKMCGLPGVPVCPNPDKYISWDGIHLTQMAYKSMAHWLIDDV 362
            SLQKSCCGIGGDYNF+ MKMCG+ GV VC NPD+ +SWDGIHLTQ AYK MA+WLI D+
Sbjct: 307 TSLQKSCCGIGGDYNFSLMKMCGVGGVSVCSNPDERVSWDGIHLTQNAYKFMAYWLIHDI 366

Query: 363 YPQLHCIV 369
           +PQLHCIV
Sbjct: 367 FPQLHCIV 374

BLAST of Lag0006549 vs. NCBI nr
Match: XP_023546436.1 (acetylajmalan esterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 608.6 bits (1568), Expect = 3.6e-170
Identity = 294/374 (78.61%), Postives = 323/374 (86.36%), Query Frame = 0

Query: 1   MAASTTP-----LLTLLFLL-LLSSSSSDAHSLKACKFDAIYQLGDSISDTGNLIRENPN 60
           MAAST       L  LL LL +L   SS AH LK C FDAIYQLGDSISDTGNLIR+NPN
Sbjct: 1   MAASTKSPSSIVLAVLLVLLSVLGCPSSKAHLLKTCMFDAIYQLGDSISDTGNLIRQNPN 60

Query: 61  TPFSHLPYGETFFKNSTGRCSNGLLMIDYFAQDIGIPLVNPYLNKDALTRHGVNFAVAGS 120
           TPFS+LPYGETFF  STGRCSNGLLMIDYFA D G+PLVNPYLNKDALTRHGVNFAVAGS
Sbjct: 61  TPFSNLPYGETFFNKSTGRCSNGLLMIDYFALDAGLPLVNPYLNKDALTRHGVNFAVAGS 120

Query: 121 TALSSEVLLEKKISAPKTNSPLDHQLDWMFSHFNSICYNQKDCNEKLKNALFLVGEIGAN 180
           TALSS++L + +I +P TNS L+ QL WMFSHFNSICYNQ+DCN+KL+NALFLVGEIG N
Sbjct: 121 TALSSQLLSQNQILSPLTNSSLNQQLYWMFSHFNSICYNQRDCNQKLRNALFLVGEIGGN 180

Query: 181 DYNYAFFQGKTIEEVKDMVPQVVQTIKSGVERVISYGAAQVVVPGNFPIGCLPVYLTRFQ 240
           DYNYA FQGKTI+EVKDMVPQVVQTIKS VE+VISYGA +VVVPGNFPIGCLP+YLT FQ
Sbjct: 181 DYNYALFQGKTIQEVKDMVPQVVQTIKSAVEKVISYGATRVVVPGNFPIGCLPIYLTGFQ 240

Query: 241 TSNTTAYDELHCLKSFNNLASYHNDQIKQAIEVLKKENPHIVIVYGDYYNAFLWILRHAS 300
           T++TTAYDELHCLK  N LA+YHNDQIKQAIEVLK+ENPH VIVYGDYYNA LWILR A 
Sbjct: 241 TNDTTAYDELHCLKDLNGLATYHNDQIKQAIEVLKRENPHTVIVYGDYYNALLWILRRAF 300

Query: 301 VLGFDEVSLQKSCCGIGGDYNFNHMKMCGLPGVPVCPNPDKYISWDGIHLTQMAYKSMAH 360
            LGFDE SLQKSCCGIGG+YNF+ MKMCG+ GV VC NPD+ +SWDGIHLTQ AYK MA+
Sbjct: 301 TLGFDEASLQKSCCGIGGNYNFSLMKMCGVGGVSVCSNPDERVSWDGIHLTQKAYKFMAY 360

Query: 361 WLIDDVYPQLHCIV 369
           WLI D++PQLHCIV
Sbjct: 361 WLIHDIFPQLHCIV 374

BLAST of Lag0006549 vs. NCBI nr
Match: XP_022946883.1 (acetylajmalan esterase-like [Cucurbita moschata])

HSP 1 Score: 608.2 bits (1567), Expect = 4.6e-170
Identity = 293/374 (78.34%), Postives = 321/374 (85.83%), Query Frame = 0

Query: 1   MAASTTP------LLTLLFLLLLSSSSSDAHSLKACKFDAIYQLGDSISDTGNLIRENPN 60
           MAAST        L+ LL L +L   SS AH LK C FDAIYQLGDSISDTGNLIR+NPN
Sbjct: 1   MAASTKSPSSIVLLVLLLLLSVLGCPSSKAHLLKTCMFDAIYQLGDSISDTGNLIRQNPN 60

Query: 61  TPFSHLPYGETFFKNSTGRCSNGLLMIDYFAQDIGIPLVNPYLNKDALTRHGVNFAVAGS 120
           TPFSHLPYGETFF  STGRCSNGLLMIDYFA D G+PLVNPYLNKDALTRHGVNFAVAGS
Sbjct: 61  TPFSHLPYGETFFNKSTGRCSNGLLMIDYFALDAGLPLVNPYLNKDALTRHGVNFAVAGS 120

Query: 121 TALSSEVLLEKKISAPKTNSPLDHQLDWMFSHFNSICYNQKDCNEKLKNALFLVGEIGAN 180
           TALSS++L + +I +P TNS L+ QL WMFSHFNSICY+Q+DCNEKL+NALF VGEIG N
Sbjct: 121 TALSSQLLSQNQILSPVTNSSLNQQLYWMFSHFNSICYDQRDCNEKLRNALFFVGEIGGN 180

Query: 181 DYNYAFFQGKTIEEVKDMVPQVVQTIKSGVERVISYGAAQVVVPGNFPIGCLPVYLTRFQ 240
           DYNYA FQGKTI EVKDMVPQVVQTIKS VE+VISYGA +VVVPGNFPIGCLP+YLT FQ
Sbjct: 181 DYNYALFQGKTIPEVKDMVPQVVQTIKSAVEKVISYGATRVVVPGNFPIGCLPIYLTGFQ 240

Query: 241 TSNTTAYDELHCLKSFNNLASYHNDQIKQAIEVLKKENPHIVIVYGDYYNAFLWILRHAS 300
           T++TTAYDELHCLK  N LA+YHNDQIKQAIEVLK+ENPH +IVYGDYYNA LWILR A 
Sbjct: 241 TNDTTAYDELHCLKDLNGLATYHNDQIKQAIEVLKRENPHTIIVYGDYYNALLWILRRAF 300

Query: 301 VLGFDEVSLQKSCCGIGGDYNFNHMKMCGLPGVPVCPNPDKYISWDGIHLTQMAYKSMAH 360
            LGFDE SLQKSCCGIGGDYNF+ MKMCG+ GV VC NPD+ +SWDGIHLTQ AYK MA+
Sbjct: 301 TLGFDETSLQKSCCGIGGDYNFSLMKMCGV-GVSVCSNPDERVSWDGIHLTQKAYKFMAY 360

Query: 361 WLIDDVYPQLHCIV 369
           WLI D++PQLHCIV
Sbjct: 361 WLIHDIFPQLHCIV 373

BLAST of Lag0006549 vs. ExPASy Swiss-Prot
Match: Q3MKY2 (Acetylajmalan esterase OS=Rauvolfia serpentina OX=4060 GN=AAE PE=1 SV=1)

HSP 1 Score: 334.0 bits (855), Expect = 2.2e-90
Identity = 167/362 (46.13%), Postives = 226/362 (62.43%), Query Frame = 0

Query: 8   LLTLLFLLLLSSSSSDAHSLKACKFDAIYQLGDSISDTGNLIRENPNTP---FSHLPYGE 67
           LL L+F LL+ +  ++      C FD+IYQLGDS SDTGNLIR  P+ P    +H PYGE
Sbjct: 6   LLHLVFSLLVFAGITNG---LICPFDSIYQLGDSFSDTGNLIRLPPDGPTFTAAHFPYGE 65

Query: 68  TFFKNSTGRCSNGLLMIDYFAQDIGIPLVNPYLNKDALTRHGVNFAVAGSTALSSEVLLE 127
           TF    TGRCS+G L+ID+ A  + +PL+NPYL ++   RHGVNFAVAG+TAL    L  
Sbjct: 66  TFPGTPTGRCSDGRLIIDFIATALNLPLLNPYLQQNVSFRHGVNFAVAGATALDRSFLAA 125

Query: 128 KKISAPKTNSPLDHQLDWMFSHFNSICYNQKDCNEKLKNALFLVGEIGANDYNYAFFQGK 187
           + +     +S L  QL+W  ++  SIC   K+C+ KLKNALF++G IG ND NYA F  +
Sbjct: 126 RGVQVSDIHSHLSAQLNWFRTYLGSICSTPKECSNKLKNALFILGNIGNNDVNYA-FPNR 185

Query: 188 TIEEVKDMVPQVVQTIKSGVERVISYGAAQVVVPGNFPIGCLPVYLTRFQTSNTTAYDEL 247
           TIEE++  VP + + + +    +I  G ++V+VPG FPIGC+   L           D+L
Sbjct: 186 TIEEIRAYVPFITEAVANATREIIRLGGSRVIVPGIFPIGCVARNLNFLNFFPDGDKDDL 245

Query: 248 HCLKSFNNLASYHNDQIKQAIEVLKKENPHIVIVYGDYYNAFLWILRHASVLGFDEVSLQ 307
            CL S NNL+ Y N   ++A+  L  E P  VI+Y DYYNA+ ++ R+   LG +  SL 
Sbjct: 246 GCLSSLNNLSIYFNSLFQRALASLSIEFPQAVIIYADYYNAWRFLFRNGPALGSNSTSLL 305

Query: 308 KSCCGIGGDYNFNHMKMCGLPGVPVCPNPDKYISWDGIHLTQMAYKSMAHWLIDDVYPQL 367
           K CCGIGG YN++  + CG  GVPVCPNP +YI WDG H TQ AY+ +A ++I  +   L
Sbjct: 306 KCCCGIGGPYNYDPDRECGSRGVPVCPNPTQYIQWDGTHFTQAAYRRVAEYVIPGIIKAL 363

BLAST of Lag0006549 vs. ExPASy Swiss-Prot
Match: Q9C857 (GDSL esterase/lipase At1g31550 OS=Arabidopsis thaliana OX=3702 GN=At1g31550 PE=2 SV=1)

HSP 1 Score: 300.1 bits (767), Expect = 3.5e-80
Identity = 160/358 (44.69%), Postives = 219/358 (61.17%), Query Frame = 0

Query: 9   LTLLFLLLLSSSSSDAHSLKACKFDAIYQLGDSISDTGNL--IRENPNTPFSHL-PYGET 68
           L+ LF+ ++SS S      +   F++I   GDSI+DTGNL  + ++ N P S   PYGET
Sbjct: 17  LSTLFVSIVSSES------QCRNFESIISFGDSIADTGNLLGLSDHNNLPMSAFPPYGET 76

Query: 69  FFKNSTGRCSNGLLMIDYFAQDIGIPLVNPYL-NKDALTRHGVNFAVAGSTALSSEVLLE 128
           FF + TGR S+G L+ID+ A+ +G+P V PY  + +     GVNFAVA +TAL S  L E
Sbjct: 77  FFHHPTGRFSDGRLIIDFIAEFLGLPYVPPYFGSTNGNFEKGVNFAVASATALESSFLEE 136

Query: 129 KKISAPKTNSPLDHQLDWMFSHFNSICYNQKDCNEKLKNALFLVGEIGANDYNYAFFQGK 188
           K    P  N  L  QL        ++C    DC + + NAL L+GEIGANDYN+ FFQ +
Sbjct: 137 KGYHCPH-NFSLGVQLKIFKQSLPNLCGLPSDCRDMIGNALILMGEIGANDYNFPFFQLR 196

Query: 189 TIEEVKDMVPQVVQTIKSGVERVISYGAAQVVVPGNFPIGCLPVYLTRFQTSNTTAYDEL 248
            ++EVK++VP V+ TI S +  +I  G    +VPG FP+GC   +LT  QTSN   YD L
Sbjct: 197 PLDEVKELVPLVISTISSAITELIGMGGRTFLVPGGFPLGCSVAFLTLHQTSNMEEYDPL 256

Query: 249 -HCLKSFNNLASYHNDQIKQAIEVLKKENPHIVIVYGDYYNAFLWILRHASVLGFDEVSL 308
             CLK  N    YH++Q+++ +  L+K NPH+ I+Y DYYNA L + R  S  GF    L
Sbjct: 257 TGCLKWLNKFGEYHSEQLQEELNRLRKLNPHVNIIYADYYNASLRLGREPSKYGFINRHL 316

Query: 309 QKSCCGIGGDYNFNHMKMCGLPGVPVCPNPDKYISWDGIHLTQMAYKSMAHWLIDDVY 362
             +CCG+GG YNFN  + CG  GV  C +P KY++WDG+H+T+ A+KSMA  L+   Y
Sbjct: 317 -SACCGVGGPYNFNLSRSCGSVGVEACSDPSKYVAWDGLHMTEAAHKSMADGLVKGPY 366

BLAST of Lag0006549 vs. ExPASy Swiss-Prot
Match: Q9LZB2 (GDSL esterase/lipase At5g03980 OS=Arabidopsis thaliana OX=3702 GN=At5g03980 PE=2 SV=1)

HSP 1 Score: 296.6 bits (758), Expect = 3.9e-79
Identity = 161/366 (43.99%), Postives = 207/366 (56.56%), Query Frame = 0

Query: 4   STTPLLTLLFLLLLSSSSSDAHSLKACKFDAIYQLGDSISDTGNLIRENPNTPFSHLPYG 63
           STT  L+LL  +L  S     HS   C  ++IYQ GDSISDTGNLIR  P +        
Sbjct: 2   STTKALSLLVFILFVSL---VHSSDQCPINSIYQFGDSISDTGNLIRNGPASS------- 61

Query: 64  ETFFKNSTGRCSNGLLMIDYFAQDIGIPLVNPYLNKDALTRHG--VNFAVAGSTALSSEV 123
                                      P   P   ++    H   VNF V+GSTAL+S  
Sbjct: 62  ---------------------------PTPKPLPQRE----HNVFVNFGVSGSTALNSSF 121

Query: 124 LLEKKISAPKTNSPLDHQLDWMFSHFNSICY-NQKDCNEKLKNALFLVGEIGANDYNYAF 183
             E+ +  P TN+PL  QL W   H  S C+ +  DC   LK++LF+VGEIG NDYNY F
Sbjct: 122 FSERNLHVPATNTPLSMQLAWFKGHLRSTCHGSSSDC---LKHSLFMVGEIGGNDYNYGF 181

Query: 184 FQGKTIEEVKDMVPQVVQTIKSGVERVISYGAAQVVVPGNFPIGCLPVYLTRFQTSNTTA 243
           FQGK +EE++  +P VV  I +    VI  GA  VVVPGNFP+GC P+YLT F   +T  
Sbjct: 182 FQGKPMEEIRSYIPHVVGAITAAAREVIRAGAVNVVVPGNFPVGCFPIYLTSFPVKDTKD 241

Query: 244 YDELHCLKSFNNLASYHNDQIKQAIEVLKKENPHIVIVYGDYYNAFLWILRHASVLGFDE 303
           YD+  CL   N  A  HN+Q+++AI  L+KE P + IVYGDYYNAF ++LR      FD+
Sbjct: 242 YDDNGCLTHLNEFAMDHNNQLQEAIASLRKEFPDVAIVYGDYYNAFQYVLRSER---FDK 301

Query: 304 VSLQKSCCGIGGDYNFNHMKMCGLPGVPVCPNPDKYISWDGIHLTQMAYKSMAHWLIDDV 363
               KSCCG GG YN++  +  G  GVPVC NP K+ISWDG+HLTQ AY+ M+ +L + +
Sbjct: 302 SVALKSCCGTGGAYNYDGKRPYGAVGVPVCQNPHKFISWDGVHLTQKAYRFMSKFLNNQI 320

Query: 364 YPQLHC 367
             Q+ C
Sbjct: 362 LSQIKC 320

BLAST of Lag0006549 vs. ExPASy Swiss-Prot
Match: Q9FXJ1 (GDSL esterase/lipase At1g28570 OS=Arabidopsis thaliana OX=3702 GN=At1g28570 PE=3 SV=1)

HSP 1 Score: 293.5 bits (750), Expect = 3.3e-78
Identity = 154/359 (42.90%), Postives = 216/359 (60.17%), Query Frame = 0

Query: 9   LTLLFLLLLSSSSSDAHSLKAC-KFDAIYQLGDSISDTGNL--IRENPNTP-FSHLPYGE 68
           L   FL+L +   +  +S   C  F +I   GDSI+DTGNL  + +  N P  + LPYGE
Sbjct: 8   LVSFFLILSTFCLTTVNSEPQCHNFKSIISFGDSIADTGNLLALSDPTNLPKVAFLPYGE 67

Query: 69  TFFKNSTGRCSNGLLMIDYFAQDIGIPLVNP-YLNKDALTRHGVNFAVAGSTALSSEVLL 128
           TFF + TGR SNG L+ID+ A+ +G PLV P Y +++A    GVNFAV G+TAL    L 
Sbjct: 68  TFFHHPTGRFSNGRLIIDFIAEFLGFPLVPPFYGSQNANFEKGVNFAVGGATALERSFLE 127

Query: 129 EKKISAPKTNSPLDHQLDWMFSHFNSICYNQKDCNEKLKNALFLVGEIGANDYNYAFFQG 188
           E+ I  P TN  L  QL        ++C +  DC + ++N+L L+GEIG NDYNYAFF G
Sbjct: 128 ERGIHFPYTNVSLAVQLSSFKESLPNLCVSPSDCRDMIENSLILMGEIGGNDYNYAFFVG 187

Query: 189 KTIEEVKDMVPQVVQTIKSGVERVISYGAAQVVVPGNFPIGCLPVYLTRFQTSNTTAYDE 248
           K IEE+K++VP V++TI S +  +I  G    +VPG FP+GC   YL+ +QTSN   YD 
Sbjct: 188 KNIEEIKELVPLVIETISSAITELIGMGGKTFLVPGEFPLGCSVAYLSLYQTSNIEEYDP 247

Query: 249 L-HCLKSFNNLASYHNDQIKQAIEVLKKENPHIVIVYGDYYNAFLWILRHASVLGFDEVS 308
           L  CLK  N  + YH++Q++  +  L+K  PH+ I+Y DYYN  L + +  +  GF    
Sbjct: 248 LTGCLKWLNKFSEYHDEQLQAELNRLQKLYPHVNIIYADYYNTLLRLAQEPAKFGFISRP 307

Query: 309 LQKSCCGIGGDYNFNHMKMCGLPGVPVCPNPDKYISWDGIHLTQMAYKSMAHWLIDDVY 362
           L  +CC +GG +NF   +  G      C +P KY+SWDG+H+T+ AY+ MA  ++   Y
Sbjct: 308 L-PACCALGGPFNFTLGRKRGTQVPECCDDPSKYVSWDGVHMTEAAYRLMAEGILKGPY 365

BLAST of Lag0006549 vs. ExPASy Swiss-Prot
Match: Q9FXJ2 (GDSL esterase/lipase At1g28580 OS=Arabidopsis thaliana OX=3702 GN=At1g28580 PE=2 SV=1)

HSP 1 Score: 292.4 bits (747), Expect = 7.4e-78
Identity = 156/369 (42.28%), Postives = 219/369 (59.35%), Query Frame = 0

Query: 1   MAASTTPLL--TLLFLLLLSSSSSDAHSLKACK-FDAIYQLGDSISDTGNLIRENPNTPF 60
           MA   +P+L   L+F+ L +   ++  S   C+ F +I   GDSI+DTGNL+  +     
Sbjct: 1   MAYPGSPILMKLLVFIFLSTFVVTNVSSETKCREFKSIISFGDSIADTGNLLGLSDPKDL 60

Query: 61  SHL---PYGETFFKNSTGRCSNGLLMIDYFAQDIGIPLVNP-YLNKDALTRHGVNFAVAG 120
            H+   PYGE FF + TGR SNG L+ID+ A+ +G+PLV P Y + +A    GVNFAV G
Sbjct: 61  PHMAFPPYGENFFHHPTGRFSNGRLIIDFIAEFLGLPLVPPFYGSHNANFEKGVNFAVGG 120

Query: 121 STALSSEVLLEKKISAPKTNSPLDHQLDWMFSHFNSICYNQKDCNEKLKNALFLVGEIGA 180
           +TAL    L ++ I  P TN  L  QL+       SIC +  DC + ++NAL L+GEIG 
Sbjct: 121 ATALERSFLEDRGIHFPYTNVSLGVQLNSFKESLPSICGSPSDCRDMIENALILMGEIGG 180

Query: 181 NDYNYAFFQGKTIEEVKDMVPQVVQTIKSGVERVISYGAAQVVVPGNFPIGCLPVYLTRF 240
           NDYNYAFF  K IEE+K+++P V+ TI S +  +I  G    +VPG FP+GC  +YLT  
Sbjct: 181 NDYNYAFFVDKGIEEIKELMPLVITTISSAITELIGMGGRTFLVPGEFPVGCSVLYLTSH 240

Query: 241 QTSNTTAYDEL-HCLKSFNNLASYHNDQIKQAIEVLKKENPHIVIVYGDYYNAFLWILRH 300
           QTSN   YD L  CLK  N     H +Q++  +  L+K  PH+ I+Y DYYNA   + + 
Sbjct: 241 QTSNMEEYDPLTGCLKWLNKFGENHGEQLRAELNRLQKLYPHVNIIYADYYNALFHLYQE 300

Query: 301 ASVLGFDEVSLQKSCCGIGGDYNFNHMKMCGLPGVPVCPNPDKYISWDGIHLTQMAYKSM 360
            +  GF    L  +CCG GG YN+   + CG   V  C +P KY++WDG+H+T+ AY+ M
Sbjct: 301 PAKFGFMNRPL-SACCGAGGPYNYTVGRKCGTDIVESCDDPSKYVAWDGVHMTEAAYRLM 360

Query: 361 AHWLIDDVY 362
           A  +++  Y
Sbjct: 361 AEGILNGPY 368

BLAST of Lag0006549 vs. ExPASy TrEMBL
Match: A0A6J1DQL3 (acetylajmalan esterase-like OS=Momordica charantia OX=3673 GN=LOC111023320 PE=3 SV=1)

HSP 1 Score: 614.8 bits (1584), Expect = 2.4e-172
Identity = 291/365 (79.73%), Postives = 322/365 (88.22%), Query Frame = 0

Query: 3   ASTTPLLTLLFLLLLSSSSSDAHSLKACKFDAIYQLGDSISDTGNLIRENPNTPFSHLPY 62
           +S + LL LLFLLL  SS+S AH L+AC FDAIYQLGDSISDTGNLIRENPNT FSHLPY
Sbjct: 8   SSFSLLLPLLFLLL--SSASTAHPLRACMFDAIYQLGDSISDTGNLIRENPNTTFSHLPY 67

Query: 63  GETFFKNSTGRCSNGLLMIDYFAQDIGIPLVNPYLNKDALTRHGVNFAVAGSTALSSEVL 122
           GETFF  +TGRCSNGLLM+DYFA D G+PLVNPYLN+DALT HGVNFAVAGSTALS E L
Sbjct: 68  GETFFHGATGRCSNGLLMLDYFALDAGLPLVNPYLNRDALTNHGVNFAVAGSTALSFEAL 127

Query: 123 LEKKISAPKTNSPLDHQLDWMFSHFNSICYNQKDCNEKLKNALFLVGEIGANDYNYAFFQ 182
           L  KI +P TNS LDHQL WM SHF SIC N++DC EKL++ALFLVGEIG NDYNYA FQ
Sbjct: 128 LRNKILSPVTNSSLDHQLGWMHSHFESICLNERDCIEKLRSALFLVGEIGGNDYNYALFQ 187

Query: 183 GKTIEEVKDMVPQVVQTIKSGVERVISYGAAQVVVPGNFPIGCLPVYLTRFQTSNTTAYD 242
           GKTI+EVKDMVP VVQTIK+ VE+VISYGA +V+VPGNFPIGCLP+YLT FQT++TTAYD
Sbjct: 188 GKTIQEVKDMVPDVVQTIKNAVEKVISYGATRVIVPGNFPIGCLPIYLTGFQTNDTTAYD 247

Query: 243 ELHCLKSFNNLASYHNDQIKQAIEVLKKENPHIVIVYGDYYNAFLWILRHASVLGFDEVS 302
           ELHCLK  N+LASYHNDQIKQAIEVLK+ENPH VIVYGDYYNA LWILRHA VLGFDE S
Sbjct: 248 ELHCLKGLNSLASYHNDQIKQAIEVLKRENPHTVIVYGDYYNALLWILRHAFVLGFDEAS 307

Query: 303 LQKSCCGIGGDYNFNHMKMCGLPGVPVCPNPDKYISWDGIHLTQMAYKSMAHWLIDDVYP 362
           LQKSCCGIGG+YNFN MK+CGLPGVPVCPNP ++ISWDGIHLTQ AYK MA+WLI D++P
Sbjct: 308 LQKSCCGIGGNYNFNLMKICGLPGVPVCPNPHEHISWDGIHLTQKAYKFMAYWLIHDIFP 367

Query: 363 QLHCI 368
           +LHCI
Sbjct: 368 KLHCI 370

BLAST of Lag0006549 vs. ExPASy TrEMBL
Match: A0A6J1G523 (acetylajmalan esterase-like OS=Cucurbita moschata OX=3662 GN=LOC111450823 PE=3 SV=1)

HSP 1 Score: 608.2 bits (1567), Expect = 2.2e-170
Identity = 293/374 (78.34%), Postives = 321/374 (85.83%), Query Frame = 0

Query: 1   MAASTTP------LLTLLFLLLLSSSSSDAHSLKACKFDAIYQLGDSISDTGNLIRENPN 60
           MAAST        L+ LL L +L   SS AH LK C FDAIYQLGDSISDTGNLIR+NPN
Sbjct: 1   MAASTKSPSSIVLLVLLLLLSVLGCPSSKAHLLKTCMFDAIYQLGDSISDTGNLIRQNPN 60

Query: 61  TPFSHLPYGETFFKNSTGRCSNGLLMIDYFAQDIGIPLVNPYLNKDALTRHGVNFAVAGS 120
           TPFSHLPYGETFF  STGRCSNGLLMIDYFA D G+PLVNPYLNKDALTRHGVNFAVAGS
Sbjct: 61  TPFSHLPYGETFFNKSTGRCSNGLLMIDYFALDAGLPLVNPYLNKDALTRHGVNFAVAGS 120

Query: 121 TALSSEVLLEKKISAPKTNSPLDHQLDWMFSHFNSICYNQKDCNEKLKNALFLVGEIGAN 180
           TALSS++L + +I +P TNS L+ QL WMFSHFNSICY+Q+DCNEKL+NALF VGEIG N
Sbjct: 121 TALSSQLLSQNQILSPVTNSSLNQQLYWMFSHFNSICYDQRDCNEKLRNALFFVGEIGGN 180

Query: 181 DYNYAFFQGKTIEEVKDMVPQVVQTIKSGVERVISYGAAQVVVPGNFPIGCLPVYLTRFQ 240
           DYNYA FQGKTI EVKDMVPQVVQTIKS VE+VISYGA +VVVPGNFPIGCLP+YLT FQ
Sbjct: 181 DYNYALFQGKTIPEVKDMVPQVVQTIKSAVEKVISYGATRVVVPGNFPIGCLPIYLTGFQ 240

Query: 241 TSNTTAYDELHCLKSFNNLASYHNDQIKQAIEVLKKENPHIVIVYGDYYNAFLWILRHAS 300
           T++TTAYDELHCLK  N LA+YHNDQIKQAIEVLK+ENPH +IVYGDYYNA LWILR A 
Sbjct: 241 TNDTTAYDELHCLKDLNGLATYHNDQIKQAIEVLKRENPHTIIVYGDYYNALLWILRRAF 300

Query: 301 VLGFDEVSLQKSCCGIGGDYNFNHMKMCGLPGVPVCPNPDKYISWDGIHLTQMAYKSMAH 360
            LGFDE SLQKSCCGIGGDYNF+ MKMCG+ GV VC NPD+ +SWDGIHLTQ AYK MA+
Sbjct: 301 TLGFDETSLQKSCCGIGGDYNFSLMKMCGV-GVSVCSNPDERVSWDGIHLTQKAYKFMAY 360

Query: 361 WLIDDVYPQLHCIV 369
           WLI D++PQLHCIV
Sbjct: 361 WLIHDIFPQLHCIV 373

BLAST of Lag0006549 vs. ExPASy TrEMBL
Match: A0A6J1G4V8 (GDSL esterase/lipase At5g03980-like OS=Cucurbita moschata OX=3662 GN=LOC111450822 PE=3 SV=1)

HSP 1 Score: 607.8 bits (1566), Expect = 2.9e-170
Identity = 284/368 (77.17%), Postives = 322/368 (87.50%), Query Frame = 0

Query: 4   STTPLLTLLFLLLLS---SSSSDAHSLKACKFDAIYQLGDSISDTGNLIRENPNTPFSHL 63
           S +P++  + ++LLS     SS AH LK C FD IYQLGDSISDTGNLI +NPNTPFSHL
Sbjct: 7   SPSPIVLAVLIVLLSVLVCPSSTAHLLKTCMFDTIYQLGDSISDTGNLILQNPNTPFSHL 66

Query: 64  PYGETFFKNSTGRCSNGLLMIDYFAQDIGIPLVNPYLNKDALTRHGVNFAVAGSTALSSE 123
           PYG++FF  STGRCSNGLLMIDYFA D G+PLVNPYLNKDA TRHGVNFAVAGSTALSS 
Sbjct: 67  PYGQSFFNKSTGRCSNGLLMIDYFALDAGLPLVNPYLNKDASTRHGVNFAVAGSTALSSH 126

Query: 124 VLLEKKISAPKTNSPLDHQLDWMFSHFNSICYNQKDCNEKLKNALFLVGEIGANDYNYAF 183
           +L +K+I +P TN+ LD QLDWMFSHFNSICYNQ+DCNEKL+NALFLVGEIG NDYNYA 
Sbjct: 127 LLSQKQILSPVTNTSLDQQLDWMFSHFNSICYNQRDCNEKLRNALFLVGEIGGNDYNYAL 186

Query: 184 FQGKTIEEVKDMVPQVVQTIKSGVERVISYGAAQVVVPGNFPIGCLPVYLTRFQTSNTTA 243
           FQGKTI+EVKDMVPQVVQTIK+ VE+VISYGA +VVVPGNFPIGCLP+YLT FQT++TTA
Sbjct: 187 FQGKTIQEVKDMVPQVVQTIKNAVEKVISYGATRVVVPGNFPIGCLPIYLTGFQTNDTTA 246

Query: 244 YDELHCLKSFNNLASYHNDQIKQAIEVLKKENPHIVIVYGDYYNAFLWILRHASVLGFDE 303
           YDELHCLK  N+LA+YHNDQIKQAI+VLK+ENPH VI+YGDYYNA LWILR A +LGFDE
Sbjct: 247 YDELHCLKDLNDLATYHNDQIKQAIQVLKRENPHSVIIYGDYYNALLWILRRAFILGFDE 306

Query: 304 VSLQKSCCGIGGDYNFNHMKMCGLPGVPVCPNPDKYISWDGIHLTQMAYKSMAHWLIDDV 363
            SLQKSCCGIGGDYNF+ +KMCG+ GV VC NPD+ +SWDGIHLTQ AYK MA+WLI D+
Sbjct: 307 ASLQKSCCGIGGDYNFSLVKMCGVGGVSVCSNPDERVSWDGIHLTQKAYKFMAYWLIHDI 366

Query: 364 YPQLHCIV 369
           +PQLHCIV
Sbjct: 367 FPQLHCIV 374

BLAST of Lag0006549 vs. ExPASy TrEMBL
Match: A0A6J1KFS3 (acetylajmalan esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111493492 PE=3 SV=1)

HSP 1 Score: 607.1 bits (1564), Expect = 5.0e-170
Identity = 287/366 (78.42%), Postives = 320/366 (87.43%), Query Frame = 0

Query: 4   STTPLLTLLFLL-LLSSSSSDAHSLKACKFDAIYQLGDSISDTGNLIRENPNTPFSHLPY 63
           S+  L  LL L+ ++   SS AH LK C FDAIYQLGDSISDTGNLIR+NPNTPFSHLPY
Sbjct: 9   SSIVLAVLLVLVSVIGCPSSTAHLLKTCMFDAIYQLGDSISDTGNLIRQNPNTPFSHLPY 68

Query: 64  GETFFKNSTGRCSNGLLMIDYFAQDIGIPLVNPYLNKDALTRHGVNFAVAGSTALSSEVL 123
           GET F  STGRCSNGLLMIDYFA D G+PLVNPYLNKDALTRHGVNFAVAGSTALSS++L
Sbjct: 69  GETLFNKSTGRCSNGLLMIDYFALDAGLPLVNPYLNKDALTRHGVNFAVAGSTALSSQLL 128

Query: 124 LEKKISAPKTNSPLDHQLDWMFSHFNSICYNQKDCNEKLKNALFLVGEIGANDYNYAFFQ 183
            +K+I +P TNS L+ QL WMFSHFNSICYNQ+DCNEKL+NALFLVGEIG NDYNYA FQ
Sbjct: 129 SQKQILSPVTNSSLNQQLYWMFSHFNSICYNQRDCNEKLRNALFLVGEIGGNDYNYALFQ 188

Query: 184 GKTIEEVKDMVPQVVQTIKSGVERVISYGAAQVVVPGNFPIGCLPVYLTRFQTSNTTAYD 243
           GKTI++VKDMVPQVVQTIK+ VE+VISYGA +VVVPGNFPIGCLP+YLT FQT++TTAYD
Sbjct: 189 GKTIQDVKDMVPQVVQTIKNAVEKVISYGATRVVVPGNFPIGCLPIYLTGFQTNDTTAYD 248

Query: 244 ELHCLKSFNNLASYHNDQIKQAIEVLKKENPHIVIVYGDYYNAFLWILRHASVLGFDEVS 303
           ELHCLK  N LA+YHNDQIK+AIEVLK+ENPH +IVYGDYYNA LWILR A +LGFDE S
Sbjct: 249 ELHCLKDLNGLATYHNDQIKEAIEVLKRENPHTMIVYGDYYNALLWILRRAFILGFDETS 308

Query: 304 LQKSCCGIGGDYNFNHMKMCGLPGVPVCPNPDKYISWDGIHLTQMAYKSMAHWLIDDVYP 363
           LQKSCCGIGGDYNF+ MKMCG+ GV VC NPD+ +SWDGIHLTQ AYK MA+WLI D+ P
Sbjct: 309 LQKSCCGIGGDYNFSLMKMCGVGGVSVCXNPDERVSWDGIHLTQKAYKFMAYWLIHDILP 368

Query: 364 QLHCIV 369
           QLHCIV
Sbjct: 369 QLHCIV 374

BLAST of Lag0006549 vs. ExPASy TrEMBL
Match: A0A6J1K9I7 (acetylajmalan esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111493500 PE=3 SV=1)

HSP 1 Score: 605.5 bits (1560), Expect = 1.5e-169
Identity = 286/366 (78.14%), Postives = 319/366 (87.16%), Query Frame = 0

Query: 4   STTPLLTLLFLL-LLSSSSSDAHSLKACKFDAIYQLGDSISDTGNLIRENPNTPFSHLPY 63
           S+  L  LL L+ ++   SS AH LK C FDAIYQLGDSISDTGNLIR+NPNTPFSHLPY
Sbjct: 9   SSIVLAVLLVLVSVIGCPSSTAHLLKTCMFDAIYQLGDSISDTGNLIRQNPNTPFSHLPY 68

Query: 64  GETFFKNSTGRCSNGLLMIDYFAQDIGIPLVNPYLNKDALTRHGVNFAVAGSTALSSEVL 123
           GET F  STGRCSNGLLMIDYFA D G+PLVNPYLNKDALTRHGVNFAVAGSTALSS++L
Sbjct: 69  GETLFNKSTGRCSNGLLMIDYFALDAGLPLVNPYLNKDALTRHGVNFAVAGSTALSSQLL 128

Query: 124 LEKKISAPKTNSPLDHQLDWMFSHFNSICYNQKDCNEKLKNALFLVGEIGANDYNYAFFQ 183
            +K+I +P TNS L+ QL WMFSHFNSICYNQ+DCNEKL+NALFLVGEIG NDYNYA FQ
Sbjct: 129 SQKQILSPVTNSSLNQQLYWMFSHFNSICYNQRDCNEKLRNALFLVGEIGGNDYNYALFQ 188

Query: 184 GKTIEEVKDMVPQVVQTIKSGVERVISYGAAQVVVPGNFPIGCLPVYLTRFQTSNTTAYD 243
           GKTI++VKDMVPQVVQTIK+ VE+ ISYGA +VVVPGNFPIGCLP+YLT FQT++TTAYD
Sbjct: 189 GKTIQDVKDMVPQVVQTIKNAVEKAISYGATRVVVPGNFPIGCLPIYLTGFQTNDTTAYD 248

Query: 244 ELHCLKSFNNLASYHNDQIKQAIEVLKKENPHIVIVYGDYYNAFLWILRHASVLGFDEVS 303
           ELHCLK  N LA+YHNDQIK+AIEVLK+ENPH +IVYGDYYNA LWILR A +LGFDE S
Sbjct: 249 ELHCLKDLNGLATYHNDQIKEAIEVLKRENPHTMIVYGDYYNALLWILRRAFILGFDETS 308

Query: 304 LQKSCCGIGGDYNFNHMKMCGLPGVPVCPNPDKYISWDGIHLTQMAYKSMAHWLIDDVYP 363
           LQKSCCGIGGDYNF+ MKMCG+ GV VC NPD+ +SWDGIHLTQ AYK MA+WLI D+ P
Sbjct: 309 LQKSCCGIGGDYNFSLMKMCGVGGVSVCSNPDERVSWDGIHLTQKAYKFMAYWLIHDILP 368

Query: 364 QLHCIV 369
           QLHCIV
Sbjct: 369 QLHCIV 374

BLAST of Lag0006549 vs. TAIR 10
Match: AT1G31550.2 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 300.1 bits (767), Expect = 2.5e-81
Identity = 160/358 (44.69%), Postives = 219/358 (61.17%), Query Frame = 0

Query: 9   LTLLFLLLLSSSSSDAHSLKACKFDAIYQLGDSISDTGNL--IRENPNTPFSHL-PYGET 68
           L+ LF+ ++SS S      +   F++I   GDSI+DTGNL  + ++ N P S   PYGET
Sbjct: 17  LSTLFVSIVSSES------QCRNFESIISFGDSIADTGNLLGLSDHNNLPMSAFPPYGET 76

Query: 69  FFKNSTGRCSNGLLMIDYFAQDIGIPLVNPYL-NKDALTRHGVNFAVAGSTALSSEVLLE 128
           FF + TGR S+G L+ID+ A+ +G+P V PY  + +     GVNFAVA +TAL S  L E
Sbjct: 77  FFHHPTGRFSDGRLIIDFIAEFLGLPYVPPYFGSTNGNFEKGVNFAVASATALESSFLEE 136

Query: 129 KKISAPKTNSPLDHQLDWMFSHFNSICYNQKDCNEKLKNALFLVGEIGANDYNYAFFQGK 188
           K    P  N  L  QL        ++C    DC + + NAL L+GEIGANDYN+ FFQ +
Sbjct: 137 KGYHCPH-NFSLGVQLKIFKQSLPNLCGLPSDCRDMIGNALILMGEIGANDYNFPFFQLR 196

Query: 189 TIEEVKDMVPQVVQTIKSGVERVISYGAAQVVVPGNFPIGCLPVYLTRFQTSNTTAYDEL 248
            ++EVK++VP V+ TI S +  +I  G    +VPG FP+GC   +LT  QTSN   YD L
Sbjct: 197 PLDEVKELVPLVISTISSAITELIGMGGRTFLVPGGFPLGCSVAFLTLHQTSNMEEYDPL 256

Query: 249 -HCLKSFNNLASYHNDQIKQAIEVLKKENPHIVIVYGDYYNAFLWILRHASVLGFDEVSL 308
             CLK  N    YH++Q+++ +  L+K NPH+ I+Y DYYNA L + R  S  GF    L
Sbjct: 257 TGCLKWLNKFGEYHSEQLQEELNRLRKLNPHVNIIYADYYNASLRLGREPSKYGFINRHL 316

Query: 309 QKSCCGIGGDYNFNHMKMCGLPGVPVCPNPDKYISWDGIHLTQMAYKSMAHWLIDDVY 362
             +CCG+GG YNFN  + CG  GV  C +P KY++WDG+H+T+ A+KSMA  L+   Y
Sbjct: 317 -SACCGVGGPYNFNLSRSCGSVGVEACSDPSKYVAWDGLHMTEAAHKSMADGLVKGPY 366

BLAST of Lag0006549 vs. TAIR 10
Match: AT5G03980.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 296.6 bits (758), Expect = 2.8e-80
Identity = 161/366 (43.99%), Postives = 207/366 (56.56%), Query Frame = 0

Query: 4   STTPLLTLLFLLLLSSSSSDAHSLKACKFDAIYQLGDSISDTGNLIRENPNTPFSHLPYG 63
           STT  L+LL  +L  S     HS   C  ++IYQ GDSISDTGNLIR  P +        
Sbjct: 2   STTKALSLLVFILFVSL---VHSSDQCPINSIYQFGDSISDTGNLIRNGPASS------- 61

Query: 64  ETFFKNSTGRCSNGLLMIDYFAQDIGIPLVNPYLNKDALTRHG--VNFAVAGSTALSSEV 123
                                      P   P   ++    H   VNF V+GSTAL+S  
Sbjct: 62  ---------------------------PTPKPLPQRE----HNVFVNFGVSGSTALNSSF 121

Query: 124 LLEKKISAPKTNSPLDHQLDWMFSHFNSICY-NQKDCNEKLKNALFLVGEIGANDYNYAF 183
             E+ +  P TN+PL  QL W   H  S C+ +  DC   LK++LF+VGEIG NDYNY F
Sbjct: 122 FSERNLHVPATNTPLSMQLAWFKGHLRSTCHGSSSDC---LKHSLFMVGEIGGNDYNYGF 181

Query: 184 FQGKTIEEVKDMVPQVVQTIKSGVERVISYGAAQVVVPGNFPIGCLPVYLTRFQTSNTTA 243
           FQGK +EE++  +P VV  I +    VI  GA  VVVPGNFP+GC P+YLT F   +T  
Sbjct: 182 FQGKPMEEIRSYIPHVVGAITAAAREVIRAGAVNVVVPGNFPVGCFPIYLTSFPVKDTKD 241

Query: 244 YDELHCLKSFNNLASYHNDQIKQAIEVLKKENPHIVIVYGDYYNAFLWILRHASVLGFDE 303
           YD+  CL   N  A  HN+Q+++AI  L+KE P + IVYGDYYNAF ++LR      FD+
Sbjct: 242 YDDNGCLTHLNEFAMDHNNQLQEAIASLRKEFPDVAIVYGDYYNAFQYVLRSER---FDK 301

Query: 304 VSLQKSCCGIGGDYNFNHMKMCGLPGVPVCPNPDKYISWDGIHLTQMAYKSMAHWLIDDV 363
               KSCCG GG YN++  +  G  GVPVC NP K+ISWDG+HLTQ AY+ M+ +L + +
Sbjct: 302 SVALKSCCGTGGAYNYDGKRPYGAVGVPVCQNPHKFISWDGVHLTQKAYRFMSKFLNNQI 320

Query: 364 YPQLHC 367
             Q+ C
Sbjct: 362 LSQIKC 320

BLAST of Lag0006549 vs. TAIR 10
Match: AT1G28570.1 (SGNH hydrolase-type esterase superfamily protein )

HSP 1 Score: 293.5 bits (750), Expect = 2.4e-79
Identity = 154/359 (42.90%), Postives = 216/359 (60.17%), Query Frame = 0

Query: 9   LTLLFLLLLSSSSSDAHSLKAC-KFDAIYQLGDSISDTGNL--IRENPNTP-FSHLPYGE 68
           L   FL+L +   +  +S   C  F +I   GDSI+DTGNL  + +  N P  + LPYGE
Sbjct: 8   LVSFFLILSTFCLTTVNSEPQCHNFKSIISFGDSIADTGNLLALSDPTNLPKVAFLPYGE 67

Query: 69  TFFKNSTGRCSNGLLMIDYFAQDIGIPLVNP-YLNKDALTRHGVNFAVAGSTALSSEVLL 128
           TFF + TGR SNG L+ID+ A+ +G PLV P Y +++A    GVNFAV G+TAL    L 
Sbjct: 68  TFFHHPTGRFSNGRLIIDFIAEFLGFPLVPPFYGSQNANFEKGVNFAVGGATALERSFLE 127

Query: 129 EKKISAPKTNSPLDHQLDWMFSHFNSICYNQKDCNEKLKNALFLVGEIGANDYNYAFFQG 188
           E+ I  P TN  L  QL        ++C +  DC + ++N+L L+GEIG NDYNYAFF G
Sbjct: 128 ERGIHFPYTNVSLAVQLSSFKESLPNLCVSPSDCRDMIENSLILMGEIGGNDYNYAFFVG 187

Query: 189 KTIEEVKDMVPQVVQTIKSGVERVISYGAAQVVVPGNFPIGCLPVYLTRFQTSNTTAYDE 248
           K IEE+K++VP V++TI S +  +I  G    +VPG FP+GC   YL+ +QTSN   YD 
Sbjct: 188 KNIEEIKELVPLVIETISSAITELIGMGGKTFLVPGEFPLGCSVAYLSLYQTSNIEEYDP 247

Query: 249 L-HCLKSFNNLASYHNDQIKQAIEVLKKENPHIVIVYGDYYNAFLWILRHASVLGFDEVS 308
           L  CLK  N  + YH++Q++  +  L+K  PH+ I+Y DYYN  L + +  +  GF    
Sbjct: 248 LTGCLKWLNKFSEYHDEQLQAELNRLQKLYPHVNIIYADYYNTLLRLAQEPAKFGFISRP 307

Query: 309 LQKSCCGIGGDYNFNHMKMCGLPGVPVCPNPDKYISWDGIHLTQMAYKSMAHWLIDDVY 362
           L  +CC +GG +NF   +  G      C +P KY+SWDG+H+T+ AY+ MA  ++   Y
Sbjct: 308 L-PACCALGGPFNFTLGRKRGTQVPECCDDPSKYVSWDGVHMTEAAYRLMAEGILKGPY 365

BLAST of Lag0006549 vs. TAIR 10
Match: AT1G31550.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 292.7 bits (748), Expect = 4.0e-79
Identity = 156/358 (43.58%), Postives = 216/358 (60.34%), Query Frame = 0

Query: 9   LTLLFLLLLSSSSSDAHSLKACKFDAIYQLGDSISDTGNL--IRENPNTPFSHL-PYGET 68
           L+ LF+ ++SS S      +   F++I   GDSI+DTGNL  + ++ N P S   PYGET
Sbjct: 17  LSTLFVSIVSSES------QCRNFESIISFGDSIADTGNLLGLSDHNNLPMSAFPPYGET 76

Query: 69  FFKNSTGRCSNGLLMIDYFAQDIGIPLVNPYL-NKDALTRHGVNFAVAGSTALSSEVLLE 128
           FF + TGR S+G L+ID+ A+ +G+P V PY  + +     GVNFAVA +TAL S  L E
Sbjct: 77  FFHHPTGRFSDGRLIIDFIAEFLGLPYVPPYFGSTNGNFEKGVNFAVASATALESSFLEE 136

Query: 129 KKISAPKTNSPLDHQLDWMFSHFNSICYNQKDCNEKLKNALFLVGEIGANDYNYAFFQGK 188
           K    P  N  L  QL        ++C    DC + + NAL L+GEIGANDYN+ FFQ +
Sbjct: 137 KGYHCPH-NFSLGVQLKIFKQSLPNLCGLPSDCRDMIGNALILMGEIGANDYNFPFFQLR 196

Query: 189 TIEEVKDMVPQVVQTIKSGVERVISYGAAQVVVPGNFPIGCLPVYLTRFQTSNTTAYDEL 248
            ++EVK++VP V+ TI S +  +I  G    +VPG FP+GC   +LT  QTSN   YD L
Sbjct: 197 PLDEVKELVPLVISTISSAITELIGMGGRTFLVPGGFPLGCSVAFLTLHQTSNMEEYDPL 256

Query: 249 -HCLKSFNNLASYHNDQIKQAIEVLKKENPHIVIVYGDYYNAFLWILRHASVLGFDEVSL 308
             CLK  N    YH++Q+++ +  L+K NPH+ I+Y DYYNA L + R    +       
Sbjct: 257 TGCLKWLNKFGEYHSEQLQEELNRLRKLNPHVNIIYADYYNASLRLGREPRFIN----RH 316

Query: 309 QKSCCGIGGDYNFNHMKMCGLPGVPVCPNPDKYISWDGIHLTQMAYKSMAHWLIDDVY 362
             +CCG+GG YNFN  + CG  GV  C +P KY++WDG+H+T+ A+KSMA  L+   Y
Sbjct: 317 LSACCGVGGPYNFNLSRSCGSVGVEACSDPSKYVAWDGLHMTEAAHKSMADGLVKGPY 363

BLAST of Lag0006549 vs. TAIR 10
Match: AT1G28580.1 (GDSL-like Lipase/Acylhydrolase superfamily protein )

HSP 1 Score: 292.4 bits (747), Expect = 5.2e-79
Identity = 156/369 (42.28%), Postives = 219/369 (59.35%), Query Frame = 0

Query: 1   MAASTTPLL--TLLFLLLLSSSSSDAHSLKACK-FDAIYQLGDSISDTGNLIRENPNTPF 60
           MA   +P+L   L+F+ L +   ++  S   C+ F +I   GDSI+DTGNL+  +     
Sbjct: 1   MAYPGSPILMKLLVFIFLSTFVVTNVSSETKCREFKSIISFGDSIADTGNLLGLSDPKDL 60

Query: 61  SHL---PYGETFFKNSTGRCSNGLLMIDYFAQDIGIPLVNP-YLNKDALTRHGVNFAVAG 120
            H+   PYGE FF + TGR SNG L+ID+ A+ +G+PLV P Y + +A    GVNFAV G
Sbjct: 61  PHMAFPPYGENFFHHPTGRFSNGRLIIDFIAEFLGLPLVPPFYGSHNANFEKGVNFAVGG 120

Query: 121 STALSSEVLLEKKISAPKTNSPLDHQLDWMFSHFNSICYNQKDCNEKLKNALFLVGEIGA 180
           +TAL    L ++ I  P TN  L  QL+       SIC +  DC + ++NAL L+GEIG 
Sbjct: 121 ATALERSFLEDRGIHFPYTNVSLGVQLNSFKESLPSICGSPSDCRDMIENALILMGEIGG 180

Query: 181 NDYNYAFFQGKTIEEVKDMVPQVVQTIKSGVERVISYGAAQVVVPGNFPIGCLPVYLTRF 240
           NDYNYAFF  K IEE+K+++P V+ TI S +  +I  G    +VPG FP+GC  +YLT  
Sbjct: 181 NDYNYAFFVDKGIEEIKELMPLVITTISSAITELIGMGGRTFLVPGEFPVGCSVLYLTSH 240

Query: 241 QTSNTTAYDEL-HCLKSFNNLASYHNDQIKQAIEVLKKENPHIVIVYGDYYNAFLWILRH 300
           QTSN   YD L  CLK  N     H +Q++  +  L+K  PH+ I+Y DYYNA   + + 
Sbjct: 241 QTSNMEEYDPLTGCLKWLNKFGENHGEQLRAELNRLQKLYPHVNIIYADYYNALFHLYQE 300

Query: 301 ASVLGFDEVSLQKSCCGIGGDYNFNHMKMCGLPGVPVCPNPDKYISWDGIHLTQMAYKSM 360
            +  GF    L  +CCG GG YN+   + CG   V  C +P KY++WDG+H+T+ AY+ M
Sbjct: 301 PAKFGFMNRPL-SACCGAGGPYNYTVGRKCGTDIVESCDDPSKYVAWDGVHMTEAAYRLM 360

Query: 361 AHWLIDDVY 362
           A  +++  Y
Sbjct: 361 AEGILNGPY 368