Lag0006460 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0006460 vs. NCBI nr
Match: KAG6586059.1 (Protein NRT1/ PTR FAMILY 4.5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1031.6 bits (2666), Expect = 3.3e-297
Identity = 519/595 (87.23%), Postives = 557/595 (93.61%), Query Frame = 0

Query: 122 GTEGREIKVVDPRDEGKGGFRATMFIFALLTFESMGFVANGVSLVQYFLLVLHFDLQTAA 181
           G+E +EI VVDP +EGKGGFRATMFIFALLTFESMGFVAN VSLVQYFL+VLHFDLQTAA
Sbjct: 3   GSEEKEINVVDPTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQTAA 62

Query: 182 NTLTNFFGSAFLLSLLGGFLSDTYINRLTTCLVFGVLEILALVMVTVQAYSHDLLPKPDC 241
           NTLTNF GSAFLLSLLGGFLSDTYINRLTTCL+FGVLE+LALV+VTVQAYSHDLLPK DC
Sbjct: 63  NTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIFGVLEVLALVIVTVQAYSHDLLPKADC 122

Query: 242 PKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFDQKDPKEAKALATFFNYMLLS 301
           PKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQF+QKDPKEAKALATFFN +LLS
Sbjct: 123 PKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLLLS 182

Query: 302 IVIGAAVGVTLIVWVAVNKAWYWGFFTSTVATAVGFIVFAIGKPFYRLHVPGQSPMLRII 361
           +VIGAAVGVT+IVWVAVNKAWYWGFF S +ATAVGFIVFA+GKPFYRLHVPGQSP+LRII
Sbjct: 183 VVIGAAVGVTMIVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLRII 242

Query: 362 QVIVVAIKNRGLALPETPTELYEISDKCYTHSIHSKIAHTNQLRFLDKAAILGKEREAEA 421
           QVIVVA+KNRGLALP+TP ELYE+SDK Y  SI SKI HTNQLR LDKA+IL K+ E + 
Sbjct: 243 QVIVVAVKNRGLALPDTPNELYELSDKYYMDSIDSKITHTNQLRCLDKASILPKDVEPQP 302

Query: 422 WKVCSVTQVEEVKIITRMLPIFFCTIIMNTCLAQLQTFSVEQGNTRIMDKSLGHLQFPAP 481
           WKVCSVTQVEEVKIITRMLPIF  TIIMNTCLAQLQTFSVEQGNT+IMDKSLGHLQFPAP
Sbjct: 303 WKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTQIMDKSLGHLQFPAP 362

Query: 482 SIPVIPLLFMTFLIPLYEFVFVPFARRITHHPSGITQLQRIGVGLVLSAISMAVAGLVEV 541
           SIPVIPL+FM FLIPLYEFVFVPFAR+ITHHPSGITQLQR+GVGLVLSA+SMAVAGLVEV
Sbjct: 363 SIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLVEV 422

Query: 542 KRRHQATTHPDKPMSLFWLSFQYGIFGVADMFTLVGLLEFFYKEAPIGMRSLSTSLTWLS 601
           KRRHQAT HPD+PMSLFWLSFQYGIFG+ADMFTLVGLLEFFYKEAP+GMRSLSTS TWLS
Sbjct: 423 KRRHQATKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLS 482

Query: 602 LSLGYYLSSIFVNVVNKVTRKITPSKKGWVEGLIPEDLNHNNLNLFYWFLAILSVLNFLH 661
           LS GYYLSSIFVNV+NK+TRK++PSKKGWVEGLIPEDLN+NNLNLFYWFLAILS+LNF H
Sbjct: 483 LSFGYYLSSIFVNVINKITRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNFFH 542

Query: 662 YLYWASWYKYKTEEPIAELNGEGGG-GEGEEGVPVLREEEGSGVDVKAHRSEEKE 716
           YLYWASWYKYKTEE I +L+  GGG  E EEGVP+LREEE SG D+K H SEEKE
Sbjct: 543 YLYWASWYKYKTEESIVKLDDGGGGEREREEGVPILREEERSGGDIKCHHSEEKE 597

BLAST of Lag0006460 vs. NCBI nr
Match: XP_022938323.1 (protein NRT1/ PTR FAMILY 4.5-like [Cucurbita moschata])

HSP 1 Score: 1030.4 bits (2663), Expect = 7.4e-297
Identity = 519/595 (87.23%), Postives = 557/595 (93.61%), Query Frame = 0

Query: 122 GTEGREIKVVDPRDEGKGGFRATMFIFALLTFESMGFVANGVSLVQYFLLVLHFDLQTAA 181
           G+E +EI VV P +EGKGGFRATMFIFALLTFESMGFVAN VSLVQYFL+VLHFDLQTAA
Sbjct: 3   GSEEKEINVVGPTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQTAA 62

Query: 182 NTLTNFFGSAFLLSLLGGFLSDTYINRLTTCLVFGVLEILALVMVTVQAYSHDLLPKPDC 241
           NTLTNF GSAFLLSLLGGFLSDTYINRLTTCL+FGVLE+LALV+VTVQAYSHDLLPK DC
Sbjct: 63  NTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIFGVLEVLALVIVTVQAYSHDLLPKADC 122

Query: 242 PKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFDQKDPKEAKALATFFNYMLLS 301
           PKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQF+QKDPKEAKALATFFN +LLS
Sbjct: 123 PKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLLLS 182

Query: 302 IVIGAAVGVTLIVWVAVNKAWYWGFFTSTVATAVGFIVFAIGKPFYRLHVPGQSPMLRII 361
           +VIGAAVGVT+IVWVAVNKAWYWGFF S +ATAVGFIVFA+GKPFYRLHVPGQSP+LRII
Sbjct: 183 VVIGAAVGVTMIVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLRII 242

Query: 362 QVIVVAIKNRGLALPETPTELYEISDKCYTHSIHSKIAHTNQLRFLDKAAILGKEREAEA 421
           QVIVVA+KNRGLALP+TP ELYE+SDK Y  SI SKIAHTNQLR LDKA+IL K+ E + 
Sbjct: 243 QVIVVAVKNRGLALPDTPNELYELSDKYYMDSIDSKIAHTNQLRCLDKASILPKDVEPQP 302

Query: 422 WKVCSVTQVEEVKIITRMLPIFFCTIIMNTCLAQLQTFSVEQGNTRIMDKSLGHLQFPAP 481
           WKVCSVTQVEEVKIITRMLPIF  TIIMNTCLAQLQTFSVEQGNT+IMDKSLGHLQFPAP
Sbjct: 303 WKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTQIMDKSLGHLQFPAP 362

Query: 482 SIPVIPLLFMTFLIPLYEFVFVPFARRITHHPSGITQLQRIGVGLVLSAISMAVAGLVEV 541
           SIPVIPL+FM FLIPLYEFVFVPFAR+ITHHPSGITQLQR+GVGLVLSA+SMAVAGLVEV
Sbjct: 363 SIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLVEV 422

Query: 542 KRRHQATTHPDKPMSLFWLSFQYGIFGVADMFTLVGLLEFFYKEAPIGMRSLSTSLTWLS 601
           KRRHQAT HPD+PMSLFWLSFQYGIFG+ADMFTLVGLLEFFYKEAP+GMRSLSTS TWLS
Sbjct: 423 KRRHQATKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLS 482

Query: 602 LSLGYYLSSIFVNVVNKVTRKITPSKKGWVEGLIPEDLNHNNLNLFYWFLAILSVLNFLH 661
           LS GYYLSSIFVNV+NK+TRK++PSKKGWVEGLIPEDLN+NNLNLFYWFLAILS+LNF H
Sbjct: 483 LSFGYYLSSIFVNVINKITRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNFFH 542

Query: 662 YLYWASWYKYKTEEPIAELNGEGGG-GEGEEGVPVLREEEGSGVDVKAHRSEEKE 716
           YLYWASWYKYKTEE I +L+  GGG  E EEGVP+LREEE SG D+K H SEEKE
Sbjct: 543 YLYWASWYKYKTEESIVKLDDGGGGEREREEGVPILREEERSGGDIKCHHSEEKE 597

BLAST of Lag0006460 vs. NCBI nr
Match: XP_023537955.1 (protein NRT1/ PTR FAMILY 4.5-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1029.2 bits (2660), Expect = 1.6e-296
Identity = 521/595 (87.56%), Postives = 558/595 (93.78%), Query Frame = 0

Query: 122 GTEGREIKVVDPRDEGKGGFRATMFIFALLTFESMGFVANGVSLVQYFLLVLHFDLQTAA 181
           G+E +EI VVDP +EGKGGFRATMFIFALLTFESMGFVAN VSLVQYFLLVLHFDLQTAA
Sbjct: 3   GSEEKEINVVDPTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLLVLHFDLQTAA 62

Query: 182 NTLTNFFGSAFLLSLLGGFLSDTYINRLTTCLVFGVLEILALVMVTVQAYSHDLLPKPDC 241
           NTLTNF GSAFLLSLLGGFLSDTYINRLTTCL+FGVLE+LALV+VTVQAYSHDLLPK DC
Sbjct: 63  NTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIFGVLEVLALVIVTVQAYSHDLLPKADC 122

Query: 242 PKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFDQKDPKEAKALATFFNYMLLS 301
           PKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQF+QKDPKEAKALATFFN +LLS
Sbjct: 123 PKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLLLS 182

Query: 302 IVIGAAVGVTLIVWVAVNKAWYWGFFTSTVATAVGFIVFAIGKPFYRLHVPGQSPMLRII 361
           +VIGAAVGVTLIVWVAVNKAWYWGFF S +ATAVGFIVFA+GKPFYRLHVPGQSP+LRII
Sbjct: 183 VVIGAAVGVTLIVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLRII 242

Query: 362 QVIVVAIKNRGLALPETPTELYEISDKCYTHSIHSKIAHTNQLRFLDKAAILGKEREAEA 421
           QVIVVAIKNRGLALP+TP+ELYE+S+K Y  SI SKIAHTNQLR LDKA+IL K+ E + 
Sbjct: 243 QVIVVAIKNRGLALPDTPSELYELSNKYYMDSIDSKIAHTNQLRCLDKASILPKDVEPQP 302

Query: 422 WKVCSVTQVEEVKIITRMLPIFFCTIIMNTCLAQLQTFSVEQGNTRIMDKSLGHLQFPAP 481
           WKVCSVTQVEEVKIITRMLPIF  TIIMNTCLAQLQTFSVEQGNT+IMDKSLGHLQFPAP
Sbjct: 303 WKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTQIMDKSLGHLQFPAP 362

Query: 482 SIPVIPLLFMTFLIPLYEFVFVPFARRITHHPSGITQLQRIGVGLVLSAISMAVAGLVEV 541
           SIPVIPL+FM FLIPLYEFVFVPFAR+ITHHPSGITQLQR+GVGLVLSA+SMAVAGLVEV
Sbjct: 363 SIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLVEV 422

Query: 542 KRRHQATTHPDKPMSLFWLSFQYGIFGVADMFTLVGLLEFFYKEAPIGMRSLSTSLTWLS 601
           KRRH+AT HP++PMSLFWLSFQYGIFG+ADMFTLVGLLEFFYKEAP+GMRSLSTS TWLS
Sbjct: 423 KRRHRATKHPNEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLS 482

Query: 602 LSLGYYLSSIFVNVVNKVTRKITPSKKGWVEGLIPEDLNHNNLNLFYWFLAILSVLNFLH 661
           LS GYYLSSIFVNV+NKVTRK++PSKKGWVEGLIPEDLN+NNLNLFYWFLAILS+LNF H
Sbjct: 483 LSFGYYLSSIFVNVINKVTRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNFFH 542

Query: 662 YLYWASWYKYKTEEPIAEL-NGEGGGGEGEEGVPVLREEEGSGVDVKAHRSEEKE 716
           YLYWASWYKYKTEE I +L NG GG  E EEGVP+LREEE SG D+K H SE KE
Sbjct: 543 YLYWASWYKYKTEETIVKLDNGGGGEREREEGVPILREEERSGGDIKCHHSEGKE 597

BLAST of Lag0006460 vs. NCBI nr
Match: XP_022969591.1 (protein NRT1/ PTR FAMILY 4.5-like [Cucurbita maxima])

HSP 1 Score: 1018.1 bits (2631), Expect = 3.8e-293
Identity = 516/595 (86.72%), Postives = 551/595 (92.61%), Query Frame = 0

Query: 122 GTEGREIKVVDPRDEGKGGFRATMFIFALLTFESMGFVANGVSLVQYFLLVLHFDLQTAA 181
           G+E +EI VVDP +EGKGGFRATMFIFALLTFESMGFVAN VSLVQYFL+VLHFDLQTAA
Sbjct: 3   GSEEKEINVVDPTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQTAA 62

Query: 182 NTLTNFFGSAFLLSLLGGFLSDTYINRLTTCLVFGVLEILALVMVTVQAYSHDLLPKPDC 241
           NTLTNF GSAFLLSLLGGFLSDTYINRLTTCL+FGVLE+LALV+VTVQAYSHDLLPK DC
Sbjct: 63  NTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIFGVLEVLALVIVTVQAYSHDLLPKADC 122

Query: 242 PKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFDQKDPKEAKALATFFNYMLLS 301
           PKDCV GRIAFVFYTSL LLAIGSGGVRGALPALGADQF+QKDPKEAKALATFFN +LLS
Sbjct: 123 PKDCVHGRIAFVFYTSLCLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLLLS 182

Query: 302 IVIGAAVGVTLIVWVAVNKAWYWGFFTSTVATAVGFIVFAIGKPFYRLHVPGQSPMLRII 361
           +VIGAAVGVTLIVWVAVNKAWYWGFF S +ATAVGFIVFA+GKPFYRLHVPGQSP+LRII
Sbjct: 183 VVIGAAVGVTLIVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLRII 242

Query: 362 QVIVVAIKNRGLALPETPTELYEISDKCYTHSIHSKIAHTNQLRFLDKAAILGKEREAEA 421
           QVIVVAIKNRGLALP TP+ELYE+SDK Y  SI SKIA T QLRFLDKA+IL K+ E + 
Sbjct: 243 QVIVVAIKNRGLALPNTPSELYELSDKYYMDSIESKIARTYQLRFLDKASILPKDVEPQP 302

Query: 422 WKVCSVTQVEEVKIITRMLPIFFCTIIMNTCLAQLQTFSVEQGNTRIMDKSLGHLQFPAP 481
           WKVCSVTQVEEVKIITRMLPIF  TIIMNTCLAQLQTFSVEQGNT+IMDKSLGHLQFPAP
Sbjct: 303 WKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTKIMDKSLGHLQFPAP 362

Query: 482 SIPVIPLLFMTFLIPLYEFVFVPFARRITHHPSGITQLQRIGVGLVLSAISMAVAGLVEV 541
           SIPVIPL+FM FLIPLYEFVFVPFAR+ITHHPSGITQLQR+GVGLVLSA+SMAVAGLVE+
Sbjct: 363 SIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLVEM 422

Query: 542 KRRHQATTHPDKPMSLFWLSFQYGIFGVADMFTLVGLLEFFYKEAPIGMRSLSTSLTWLS 601
           KRRHQAT HPD+PMSLFWLSFQYGIFG+ADMFTLVGLLEFFYKEAP+GMRSLSTS TWLS
Sbjct: 423 KRRHQATKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLS 482

Query: 602 LSLGYYLSSIFVNVVNKVTRKITPSKKGWVEGLIPEDLNHNNLNLFYWFLAILSVLNFLH 661
           LS GYYLSSIFVNV+NKVTRK++PSKKGWVEGLIPEDLN+NNLNLFYWFLAILS+LNF H
Sbjct: 483 LSFGYYLSSIFVNVINKVTRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNFFH 542

Query: 662 YLYWASWYKYKTEEPIAELNGEGGG-GEGEEGVPVLREEEGSGVDVKAHRSEEKE 716
           YLYWASWYKYKTEEPI +LN  GGG  E EE VP+L EE   G  +K H SEEKE
Sbjct: 543 YLYWASWYKYKTEEPIVKLNDGGGGEREREESVPILHEERSGGA-IKCHHSEEKE 596

BLAST of Lag0006460 vs. NCBI nr
Match: XP_022156380.1 (LOW QUALITY PROTEIN: protein NRT1/ PTR FAMILY 4.5-like [Momordica charantia])

HSP 1 Score: 998.8 bits (2581), Expect = 2.4e-287
Identity = 517/627 (82.46%), Postives = 556/627 (88.68%), Query Frame = 0

Query: 122 GTEGREIKVVDPRDEGKGGFRATMFIFALLTFESMGFVANGVSLVQYFLLVLHFDLQTAA 181
           G+E REIK VDP ++GKGGFRAT+FIFALL+FE+MGFVAN VSLVQYFLLV+HFDLQTAA
Sbjct: 71  GSEAREIKDVDPSNKGKGGFRATIFIFALLSFENMGFVANMVSLVQYFLLVMHFDLQTAA 130

Query: 182 NTLTNFFGSAFLLSLLGGFLSDTYINRLTTCLVFGVLEILALVMVTVQAYSHDLLPKPDC 241
           NTLTNF GSAFLLSLLGGFLSDTYINRLTT L+FGVLEILALVM+TVQAYS DLLPKPDC
Sbjct: 131 NTLTNFLGSAFLLSLLGGFLSDTYINRLTTSLIFGVLEILALVMITVQAYSQDLLPKPDC 190

Query: 242 PKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFDQKDPKEAKALATFFNYMLLS 301
            KDCVRGRIAFVFYTSLYLLA+GSGGVRGALPALGA QFDQKDP+EAKAL TFFN MLLS
Sbjct: 191 LKDCVRGRIAFVFYTSLYLLAVGSGGVRGALPALGAXQFDQKDPREAKALPTFFNCMLLS 250

Query: 302 IVIGAAVGVTLIVWVAVNKAWYWGFFTSTVATAVGFIVFAIGKPFYRLHVPGQSPMLRII 361
           +V+GAAVGVT+IVWVAVNKAW+WGF  S VATAVGF+VFA+GKPFYRLHVPG+SP+ RII
Sbjct: 251 VVVGAAVGVTVIVWVAVNKAWFWGFLISAVATAVGFVVFAVGKPFYRLHVPGESPVSRII 310

Query: 362 QVIVVAIKNRGLALPETPTELYEISDKCYTHSIHSKIAHTNQLRFLDKAAILGKEREAEA 421
           QVIVVAI+NR L LP++P+ELYEISDK Y  SI SKIAHTNQLRFLDKAAIL  + EA+A
Sbjct: 311 QVIVVAIRNRRLPLPDSPSELYEISDKYYLDSIDSKIAHTNQLRFLDKAAILPNDSEAQA 370

Query: 422 WKVCSVTQVEEVKIITRMLPIFFCTIIMNTCLAQLQTFSVEQGNTRIMDKSLGHLQFPAP 481
           WKVCSVTQVEEVKIITRM+PIF  TIIMNTCLAQLQTFSVEQGNT IMD+SLGHLQFPAP
Sbjct: 371 WKVCSVTQVEEVKIITRMVPIFISTIIMNTCLAQLQTFSVEQGNTTIMDRSLGHLQFPAP 430

Query: 482 SIPVIPLLFMTFLIPLYEFVFVPFARRITHHPSGITQLQRIGVGLVLSAISMAVAGLVEV 541
           SIPVIPL+FM FLIPLYEFVFVPFAR+ITHHPSGITQLQR+GVGLVLSAISMAVAGLVEV
Sbjct: 431 SIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMAVAGLVEV 490

Query: 542 KRRHQATTHPDKPMSLFWLSFQYGIFGVADMFTLVGLLEFFYKEAPIGMRSLSTSLTWLS 601
           KRR+QAT HP++PMSLFWLSFQYGIFG+ADMFTLVGLLEFFYKEAP+GMRSLSTS TWLS
Sbjct: 491 KRRNQATNHPNQPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLS 550

Query: 602 LSLGYYLSSIFVNVVNKVTRKITPSKKGWVEGLIPEDLNHNNLNLFYWFLAILSVLNFLH 661
           LS GYYLSSIFVNVVNKVTR I PSKKGWVEG IPEDLNHNNLNLFYWFLAILS+LNF H
Sbjct: 551 LSFGYYLSSIFVNVVNKVTRNIAPSKKGWVEGQIPEDLNHNNLNLFYWFLAILSILNFFH 610

Query: 662 YLYWASWYKYKTEEPIAEL-----------------NGEGGG-------------GEGEE 718
           YLYWASWYKYKTEEPIAEL                 NG GGG             GEGEE
Sbjct: 611 YLYWASWYKYKTEEPIAELDGGGGEDAPIVQDNRASNGGGGGEEGASIIQDNRAEGEGEE 670

BLAST of Lag0006460 vs. ExPASy Swiss-Prot
Match: Q8VYE4 (Protein NRT1/ PTR FAMILY 4.5 OS=Arabidopsis thaliana OX=3702 GN=NPF4.5 PE=2 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 1.3e-115
Identity = 236/550 (42.91%), Postives = 341/550 (62.00%), Query Frame = 0

Query: 139 GGFRATMFIFALLTFESMGFVANGVSLVQYFLLVLHFDLQTAANTLTNFFGSAFLLSLLG 198
           GG  A  F+ A+   E++ F+AN  +LV Y    +H  L  +++ +T F  +AFLL+LLG
Sbjct: 29  GGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEVTTFMATAFLLALLG 88

Query: 199 GFLSDTYINRLTTCLVFGVLEILALVMVTVQAYSHDLLPKPDCPK------DCVRGRIAF 258
           GFL+D + +     L+   +E L L+++T+QA    L+P P C        + V G  A 
Sbjct: 89  GFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMP-PPCKSSAALRCEVVGGSKAA 148

Query: 259 VFYTSLYLLAIGSGGVRGALPALGADQFDQKDPKEAKALATFFNYMLLSIVIGAAVGVTL 318
             +  LYL+++G GG++G+LP+ GA+QFD+  PK  K  +TFFNY +  +  GA V VT 
Sbjct: 149 FLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAVTF 208

Query: 319 IVWVAVNKAWYWGFFTSTVATAVGFIVFAIGKPFYRLHVPGQSPMLRIIQVI----VVAI 378
           +VW+  NK W WGF  ST++  +  +VF +G  FY+  +P  SP+  I +V+    +V+ 
Sbjct: 209 VVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSPLTTIFKVLLAASIVSC 268

Query: 379 KNRGLALPETPTELY-EISDKCYTHSIHSKIAHTNQLRFLDKAAILGKEREAEAWKVCSV 438
            ++  +   T  E+  E  +K  + S+      TN L  L+K AI GK      W  C+V
Sbjct: 269 SSKTSSNHFTSREVQSEHEEKTPSQSL------TNSLTCLNK-AIEGKTH--HIWLECTV 328

Query: 439 TQVEEVKIITRMLPIFFCTIIMNTCLAQLQTFSVEQGNTRIMDKSLGHLQFPAPSIPVIP 498
            QVE+VKI+ +MLPIF CTI++N CLAQL T+SV Q  T  M++ + +   P+ S+PV P
Sbjct: 329 QQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAAT--MNRKIVNFNVPSASLPVFP 388

Query: 499 LLFMTFLIPLYEFVFVPFARRITHHPSGITQLQRIGVGLVLSAISMAVAGLVEVKRRHQA 558
           ++FM  L P Y+ + +PFAR++T    GIT LQRIGVGLVLS ++MAVA LVE+KR+  A
Sbjct: 389 VVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVA 448

Query: 559 ------TTHPDKPMSLFWLSFQYGIFGVADMFTLVGLLEFFYKEAPIGMRSLSTSLTWLS 618
                  +    P++  W++ QY   G AD+FTL GLLEFF+ EAP  MRSL+TSL+W S
Sbjct: 449 REAGLLDSEETLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWAS 508

Query: 619 LSLGYYLSSIFVNVVNKVTRKITPSKKGWVEGLIPEDLNHNNLNLFYWFLAILSVLNFLH 672
           L+LGYYLSS+ V +VN+VT+  +  +  W    + E LN N L+LFYW + +LSV+NFLH
Sbjct: 509 LALGYYLSSVMVPIVNRVTK--SAGQSPW----LGEKLNRNRLDLFYWLMCVLSVVNFLH 560

BLAST of Lag0006460 vs. ExPASy Swiss-Prot
Match: Q8H157 (Protein NRT1/ PTR FAMILY 4.6 OS=Arabidopsis thaliana OX=3702 GN=NPF4.6 PE=1 SV=1)

HSP 1 Score: 409.1 bits (1050), Expect = 1.1e-112
Identity = 227/565 (40.18%), Postives = 338/565 (59.82%), Query Frame = 0

Query: 139 GGFRATMFIFALLTFESMGFVANGVSLVQYFLLVLHFDLQTAANTLTNFFGSAFLLSLLG 198
           GG  A  F+  +   E++ ++AN  +LV Y    +H     +AN +TNF G+AFLL+LLG
Sbjct: 27  GGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMSPSKSANDVTNFMGTAFLLALLG 86

Query: 199 GFLSDTYINRLTTCLVFGVLEILALVMVTVQAYSHDLLPKPDCPK---DCVRGRIAFVFY 258
           GFLSD + +     L+   +E L L+++T+QA +  L+P P C     + V G  A + +
Sbjct: 87  GFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSLMP-PSCDSPTCEEVSGSKAAMLF 146

Query: 259 TSLYLLAIGSGGVRGALPALGADQFDQKDPKEAKALATFFNYMLLSIVIGAAVGVTLIVW 318
             LYL+A+G GG++G+L + GA+QFD+  PK  K  +TFFNY +  +  GA V VT +VW
Sbjct: 147 VGLYLVALGVGGIKGSLASHGAEQFDESTPKGRKQRSTFFNYFVFCLACGALVAVTFVVW 206

Query: 319 VAVNKAWYWGFFTSTVATAVGFIVFAIGKPFYRLHVPGQSPMLRIIQVIVVA-------- 378
           +  NK W WGF  ST+A  V  ++F  G  FYR  +P  SP+  I++V++ A        
Sbjct: 207 LEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNKIPCGSPLTTILKVLLAASVKCCSSG 266

Query: 379 -IKNRGLALPETPT------------ELYEISDKCYTHSIHSKIAHTNQLRFLDKAAILG 438
              N   ++  +P+               E+       ++  +   TN L+ L+ AA   
Sbjct: 267 SSSNAVASMSVSPSNHCVSKGKKEVESQGELEKPRQEEALPPRAQLTNSLKVLNGAA--- 326

Query: 439 KEREAEAWKVCSVTQVEEVKIITRMLPIFFCTIIMNTCLAQLQTFSVEQGNTRIMDKSLG 498
            E+       C+V QVE+VKI+ +MLPIF CTI++N CLAQL TFSV+Q  +  M+  +G
Sbjct: 327 DEKPVHRLLECTVQQVEDVKIVLKMLPIFACTIMLNCCLAQLSTFSVQQAAS--MNTKIG 386

Query: 499 HLQFPAPSIPVIPLLFMTFLIPLYEFVFVPFARRITHHPSGITQLQRIGVGLVLSAISMA 558
            L+ P  S+P+ P++F+  L P+Y+ + +PFAR+ T   +G+T LQRIGVGLVLS ++MA
Sbjct: 387 SLKIPPASLPIFPVVFIMILAPIYDHLIIPFARKATKTETGVTHLQRIGVGLVLSILAMA 446

Query: 559 VAGLVEVKRRHQA------TTHPDKPMSLFWLSFQYGIFGVADMFTLVGLLEFFYKEAPI 618
           VA LVE+KR+  A       +    P++  W++ QY   G AD+FTL GLLE+F+ EAP 
Sbjct: 447 VAALVEIKRKGVAKDSGLLDSKETLPVTFLWIALQYLFLGSADLFTLAGLLEYFFTEAPS 506

Query: 619 GMRSLSTSLTWLSLSLGYYLSSIFVNVVNKVTRKITPSKKGWVEGLIPEDLNHNNLNLFY 674
            MRSL+TSL+W SL++GYYLSS+ V++VN +T   +     W+ G   + +N   L+ FY
Sbjct: 507 SMRSLATSLSWASLAMGYYLSSVIVSIVNSITG--SSGNTPWLRG---KSINRYKLDYFY 566

BLAST of Lag0006460 vs. ExPASy Swiss-Prot
Match: Q93VV5 (Protein NRT1/ PTR FAMILY 4.3 OS=Arabidopsis thaliana OX=3702 GN=NPF4.3 PE=2 SV=1)

HSP 1 Score: 398.7 bits (1023), Expect = 1.4e-109
Identity = 229/540 (42.41%), Postives = 328/540 (60.74%), Query Frame = 0

Query: 139 GGFRATMFIFALLTFESMGFVANGVSLVQYFLLVLHFDLQTAANTLTNFFGSAFLLSLLG 198
           GG RA +F+  L  FE MG  A G +L+ Y +  +HF L  AAN +TNF G+ F+ +LLG
Sbjct: 43  GGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSKAANIVTNFVGTIFIFALLG 102

Query: 199 GFLSDTYINRLTTCLVFGVLEILALVMVTVQAYSHDLLP---KPDCPKDC--VRGRIAFV 258
           G+LSD ++    T ++FG +E+   ++++VQA+   L P    P   + C   +G  A +
Sbjct: 103 GYLSDAFLGSFWTIIIFGFVELSGFILLSVQAHLPQLKPPKCNPLIDQTCEEAKGFKAMI 162

Query: 259 FYTSLYLLAIGSGGVRGALPALGADQFDQKDPKEAKALATFFNYMLLSIVIGAAVGVTLI 318
           F+ +LYL+A+GSG V+  + A GADQF Q  PK++K L+++FN    +  +G  + +TL+
Sbjct: 163 FFMALYLVALGSGCVKPNMIAHGADQFSQSHPKQSKRLSSYFNAAYFAFSMGELIALTLL 222

Query: 319 VWVAVNKAWYWGFFTSTVATAVGFIVFAIGKPFYRLHVPGQSPMLRIIQVIVVAIKNRGL 378
           VWV  +     GF  S  A  +G I    G  ++R   P +S    I  VIV AI  R L
Sbjct: 223 VWVQTHSGMDIGFGVSAAAMTMGIISLVSGTMYFRNKRPRRSIFTPIAHVIVAAILKRKL 282

Query: 379 ALPETPTELYEISDKCYTHSIHSKIAHTNQLRFLDKAAILGKEREAE--AWKVCSVTQVE 438
           A P  P  L+            S + HT + RFLDKA I  ++   +   W++C+VTQVE
Sbjct: 283 ASPSDPRMLHGDHHVANDVVPSSTLPHTPRFRFLDKACIKIQDTNTKESPWRLCTVTQVE 342

Query: 439 EVKIITRMLPIFFCTIIMNTCLAQLQTFSVEQG---NTRIMDKSLGHLQFPAPSIPVIPL 498
           +VK +  ++PIF  TI+ NT LAQLQTFSV+QG   NTR+ +        P  S+  IP 
Sbjct: 343 QVKTLISLVPIFASTIVFNTILAQLQTFSVQQGSSMNTRLSNS----FHIPPASLQAIPY 402

Query: 499 LFMTFLIPLYEFVFVPFARRITHHPSGITQLQRIGVGLVLSAISMAVAGLVEVKRRHQAT 558
           + + FL+PLY+   VPFAR++T H SGI  L RIG+GL LS  SM  A ++E KRR  ++
Sbjct: 403 IMLIFLVPLYDSFLVPFARKLTGHNSGIPPLTRIGIGLFLSTFSMVSAAMLEKKRR-DSS 462

Query: 559 THPDKPMSLFWLSFQYGIFGVADMFTLVGLLEFFYKEAPIGMRSLSTSLTWLSLSLGYYL 618
               + +S+FW++ Q+ IFG+++MFT VGL+EFFYK++  GM S   +LT+ S S G+Y 
Sbjct: 463 VLDGRILSIFWITPQFLIFGISEMFTAVGLIEFFYKQSAKGMESFLMALTYCSYSFGFYF 522

Query: 619 SSIFVNVVNKVTRKITPSKKGWVEGLIPEDLNHNNLNLFYWFLAILSVLNFLHYLYWASW 669
           SS+ V+VVNK+T     S KGW   L   DLN + L+LFYW LA+LS+LNFL YL+W+ W
Sbjct: 523 SSVLVSVVNKITSTSVDS-KGW---LGENDLNKDRLDLFYWLLAVLSLLNFLSYLFWSRW 573

BLAST of Lag0006460 vs. ExPASy Swiss-Prot
Match: Q56XQ6 (Protein NRT1/ PTR FAMILY 4.4 OS=Arabidopsis thaliana OX=3702 GN=NPF4.4 PE=2 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 2.5e-106
Identity = 234/549 (42.62%), Postives = 329/549 (59.93%), Query Frame = 0

Query: 134 RDEGKGGFRATMFIFALLTFESMGFVANGVSLVQYFLLVLHFDLQTAANTLTNFFGSAFL 193
           R    GG RA +F+     FE M   A G +L+ Y    +HF L  +AN +TNF G+ FL
Sbjct: 35  RPSKHGGTRAALFVLGFQAFEMMAIAAVGNNLITYVFNEMHFPLSKSANLVTNFIGTVFL 94

Query: 194 LSLLGGFLSDTYINRLTTCLVFGVLEILALVMVTVQAYSHDLLPKPDCPK-----DCV-- 253
           LSLLGGFLSD+Y+    T LVFGV+EI   ++++VQA+  +L P P+C        CV  
Sbjct: 95  LSLLGGFLSDSYLGSFRTMLVFGVIEISGFILLSVQAHLPELRP-PECNMKSTTIHCVEA 154

Query: 254 RGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFDQKDPKEAKALATFFNYMLLSIVIGA 313
            G  A   YT+L L+A+GSG ++  + + GA+QF +KD    + L++FFN    +  +G 
Sbjct: 155 NGYKAATLYTALCLVALGSGCLKPNIISHGANQFQRKD---LRKLSSFFNAAYFAFSMGQ 214

Query: 314 AVGVTLIVWVAVNKAWYWGFFTSTVATAVGFIVFAIGKPFYRLHVPGQSPMLRIIQVIVV 373
            + +TL+VWV  +     GF  S    A G I    G  FYR   P  S    I QV V 
Sbjct: 215 LIALTLLVWVQTHSGMDVGFGVSAAVMAAGMISLVAGTSFYRNKPPSGSIFTPIAQVFVA 274

Query: 374 AIKNRGLALPETPTELYEISDKCYTHSIHSK-IAHTNQLRFLDKAAILGKEREAEA-WKV 433
           AI  R    P  P  +++ S    T  +  K + H+N+ RFLDKA I  + +  E+ W++
Sbjct: 275 AITKRKQICPSNPNMVHQPS----TDLVRVKPLLHSNKFRFLDKACIKTQGKAMESPWRL 334

Query: 434 CSVTQVEEVKIITRMLPIFFCTIIMNTCLAQLQTFSVEQG---NTRIMDKSLGHLQFPAP 493
           C++ QV +VKI+  ++PIF CTII NT LAQLQTFSV+QG   NT I        Q P  
Sbjct: 335 CTIEQVHQVKILLSVIPIFACTIIFNTILAQLQTFSVQQGSSMNTHITKT----FQIPPA 394

Query: 494 SIPVIPLLFMTFLIPLYEFVFVPFARRITHHPSGITQLQRIGVGLVLSAISMAVAGLVEV 553
           S+  IP + + F +PLYE  FVP AR++T + SGI+ LQRIG GL L+  SM  A LVE 
Sbjct: 395 SLQAIPYIILIFFVPLYETFFVPLARKLTGNDSGISPLQRIGTGLFLATFSMVAAALVEK 454

Query: 554 KRRHQATTHPDKPMSLFWLSFQYGIFGVADMFTLVGLLEFFYKEAPIGMRSLSTSLTWLS 613
           KRR ++    +  +S+FW++ Q+ IFG+++MFT VGL+EFFYK++   M+S  T++T+ S
Sbjct: 455 KRR-ESFLEQNVMLSIFWIAPQFLIFGLSEMFTAVGLVEFFYKQSSQSMQSFLTAMTYCS 514

Query: 614 LSLGYYLSSIFVNVVNKVTRKI-TPSKKGWVEGLIPEDLNHNNLNLFYWFLAILSVLNFL 670
            S G+YLSS+ V+ VN+VT    + +K+GW   L   DLN + L+ FYW LA LS +NF 
Sbjct: 515 YSFGFYLSSVLVSTVNRVTSSNGSGTKEGW---LGDNDLNKDRLDHFYWLLASLSFINFF 567

BLAST of Lag0006460 vs. ExPASy Swiss-Prot
Match: Q9LSE8 (Protein NRT1/ PTR FAMILY 4.2 OS=Arabidopsis thaliana OX=3702 GN=NPF4.2 PE=2 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 3.2e-101
Identity = 212/535 (39.63%), Postives = 327/535 (61.12%), Query Frame = 0

Query: 139 GGFRATMFIFALLTFESMGFVANGVSLVQYFLLVLHFDLQTAANTLTNFFGSAFLLSLLG 198
           GG RA   +  ++  E++ F+ANG + V+YF+  +H+   TAAN +TNF G++FLL+L G
Sbjct: 23  GGIRAASIVCVVVMMENIVFIANGFNFVKYFMGSMHYTPATAANMVTNFMGTSFLLTLFG 82

Query: 199 GFLSDTYINRLTTCLVFGVLEILALVMVTVQAYSHDLLPKPDCPKDCVRGRIAFVFYTSL 258
           GF++D+++   TT +VF  +E++ L+++T QA++  LLP+ D     ++  I F   T L
Sbjct: 83  GFIADSFVTHFTTFIVFCCIELMGLILLTFQAHNPKLLPEKDKTPSTLQSAILF---TGL 142

Query: 259 YLLAIGSGGVRGALPALGADQFDQKDPKEAKALATFFNYMLLSIVIGAAVGVTLIVWVAV 318
           Y +AIG+GG++ +LP+ G DQ D+++P   + ++ FF+++  SI  G  + VT+++W+  
Sbjct: 143 YAMAIGTGGLKASLPSHGGDQIDRRNP---RLISRFFDWLYFSICSGCLLAVTVVLWIEE 202

Query: 319 NKAWYWGFFTSTVATAVGFIVFAIGKPFYRLHVPGQSPMLRIIQVIVVAIKNRGLALPET 378
            K W W F  S    A    +F +G PFYR   P  SP+ +I  VI+ A +NR  +  + 
Sbjct: 203 KKGWIWSFNISVGILATALCIFTVGLPFYRFKRPNGSPLKKIAIVIISAARNRNKS--DL 262

Query: 379 PTELYEISDKCYTHSIHSKIAHTNQLRFLDKAAILGKEREAEAWKVCSVTQVEEVKIITR 438
             E+       Y ++ H      N+L+++DKA +          K  S T+VEE +    
Sbjct: 263 DEEMMRGLISIYKNNSH------NKLKWIDKATLN---------KNISETEVEETRTFLG 322

Query: 439 MLPIFFCTIIMNTCLAQLQTFSVEQGNTRIMDKSLGH-LQFPAPSIPVIPLLFMTFLIPL 498
           +LPIF  TI+M+ C+AQL TFS +QG   +M+K L H  + P PS+  IPL+FM   IPL
Sbjct: 323 LLPIFGSTIVMSCCVAQLSTFSAQQG--MLMNKKLFHSFEIPVPSLTAIPLIFMLLSIPL 382

Query: 499 YEFVFVPFARRIT---HHPSGITQLQRIGVGLVLSAISMAVAGLVEVKRRHQATTHPDKP 558
           YEF    F ++I+   ++ S    L+RIG+GL LS++SMAV+ +VE KR+H+   H +  
Sbjct: 383 YEF----FGKKISSGNNNRSSSFNLKRIGLGLALSSVSMAVSAIVEAKRKHE-VVHNNFR 442

Query: 559 MSLFWLSFQYGIFGVADMFTLVGLLEFFYKEAPIGMRSLSTSLTWLSLSLGYYLSSIFVN 618
           +S+ WL FQY +  V+DM TL G+LEFFY+EAP  M+S+ST+L W S +LG++LS+  V 
Sbjct: 443 ISVLWLVFQYLMLSVSDMLTLGGMLEFFYREAPSNMKSISTALGWCSTALGFFLSTTLVE 502

Query: 619 VVNKVTRKITPSKKGWVEGLIPEDLNHNNLNLFYWFLAILSVLNFLHYLYWASWY 670
           V N VT ++      W+ G   EDLN   L LFY  L +L+ LN L+Y++WA  Y
Sbjct: 503 VTNAVTGRLGHQ---WLGG---EDLNKTRLELFYVLLCVLNTLNLLNYIFWAKRY 521

BLAST of Lag0006460 vs. ExPASy TrEMBL
Match: A0A6J1FIK0 (protein NRT1/ PTR FAMILY 4.5-like OS=Cucurbita moschata OX=3662 GN=LOC111444459 PE=3 SV=1)

HSP 1 Score: 1030.4 bits (2663), Expect = 3.6e-297
Identity = 519/595 (87.23%), Postives = 557/595 (93.61%), Query Frame = 0

Query: 122 GTEGREIKVVDPRDEGKGGFRATMFIFALLTFESMGFVANGVSLVQYFLLVLHFDLQTAA 181
           G+E +EI VV P +EGKGGFRATMFIFALLTFESMGFVAN VSLVQYFL+VLHFDLQTAA
Sbjct: 3   GSEEKEINVVGPTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQTAA 62

Query: 182 NTLTNFFGSAFLLSLLGGFLSDTYINRLTTCLVFGVLEILALVMVTVQAYSHDLLPKPDC 241
           NTLTNF GSAFLLSLLGGFLSDTYINRLTTCL+FGVLE+LALV+VTVQAYSHDLLPK DC
Sbjct: 63  NTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIFGVLEVLALVIVTVQAYSHDLLPKADC 122

Query: 242 PKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFDQKDPKEAKALATFFNYMLLS 301
           PKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQF+QKDPKEAKALATFFN +LLS
Sbjct: 123 PKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLLLS 182

Query: 302 IVIGAAVGVTLIVWVAVNKAWYWGFFTSTVATAVGFIVFAIGKPFYRLHVPGQSPMLRII 361
           +VIGAAVGVT+IVWVAVNKAWYWGFF S +ATAVGFIVFA+GKPFYRLHVPGQSP+LRII
Sbjct: 183 VVIGAAVGVTMIVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLRII 242

Query: 362 QVIVVAIKNRGLALPETPTELYEISDKCYTHSIHSKIAHTNQLRFLDKAAILGKEREAEA 421
           QVIVVA+KNRGLALP+TP ELYE+SDK Y  SI SKIAHTNQLR LDKA+IL K+ E + 
Sbjct: 243 QVIVVAVKNRGLALPDTPNELYELSDKYYMDSIDSKIAHTNQLRCLDKASILPKDVEPQP 302

Query: 422 WKVCSVTQVEEVKIITRMLPIFFCTIIMNTCLAQLQTFSVEQGNTRIMDKSLGHLQFPAP 481
           WKVCSVTQVEEVKIITRMLPIF  TIIMNTCLAQLQTFSVEQGNT+IMDKSLGHLQFPAP
Sbjct: 303 WKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTQIMDKSLGHLQFPAP 362

Query: 482 SIPVIPLLFMTFLIPLYEFVFVPFARRITHHPSGITQLQRIGVGLVLSAISMAVAGLVEV 541
           SIPVIPL+FM FLIPLYEFVFVPFAR+ITHHPSGITQLQR+GVGLVLSA+SMAVAGLVEV
Sbjct: 363 SIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLVEV 422

Query: 542 KRRHQATTHPDKPMSLFWLSFQYGIFGVADMFTLVGLLEFFYKEAPIGMRSLSTSLTWLS 601
           KRRHQAT HPD+PMSLFWLSFQYGIFG+ADMFTLVGLLEFFYKEAP+GMRSLSTS TWLS
Sbjct: 423 KRRHQATKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLS 482

Query: 602 LSLGYYLSSIFVNVVNKVTRKITPSKKGWVEGLIPEDLNHNNLNLFYWFLAILSVLNFLH 661
           LS GYYLSSIFVNV+NK+TRK++PSKKGWVEGLIPEDLN+NNLNLFYWFLAILS+LNF H
Sbjct: 483 LSFGYYLSSIFVNVINKITRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNFFH 542

Query: 662 YLYWASWYKYKTEEPIAELNGEGGG-GEGEEGVPVLREEEGSGVDVKAHRSEEKE 716
           YLYWASWYKYKTEE I +L+  GGG  E EEGVP+LREEE SG D+K H SEEKE
Sbjct: 543 YLYWASWYKYKTEESIVKLDDGGGGEREREEGVPILREEERSGGDIKCHHSEEKE 597

BLAST of Lag0006460 vs. ExPASy TrEMBL
Match: A0A6J1HY86 (protein NRT1/ PTR FAMILY 4.5-like OS=Cucurbita maxima OX=3661 GN=LOC111468570 PE=3 SV=1)

HSP 1 Score: 1018.1 bits (2631), Expect = 1.8e-293
Identity = 516/595 (86.72%), Postives = 551/595 (92.61%), Query Frame = 0

Query: 122 GTEGREIKVVDPRDEGKGGFRATMFIFALLTFESMGFVANGVSLVQYFLLVLHFDLQTAA 181
           G+E +EI VVDP +EGKGGFRATMFIFALLTFESMGFVAN VSLVQYFL+VLHFDLQTAA
Sbjct: 3   GSEEKEINVVDPTNEGKGGFRATMFIFALLTFESMGFVANMVSLVQYFLMVLHFDLQTAA 62

Query: 182 NTLTNFFGSAFLLSLLGGFLSDTYINRLTTCLVFGVLEILALVMVTVQAYSHDLLPKPDC 241
           NTLTNF GSAFLLSLLGGFLSDTYINRLTTCL+FGVLE+LALV+VTVQAYSHDLLPK DC
Sbjct: 63  NTLTNFLGSAFLLSLLGGFLSDTYINRLTTCLIFGVLEVLALVIVTVQAYSHDLLPKADC 122

Query: 242 PKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFDQKDPKEAKALATFFNYMLLS 301
           PKDCV GRIAFVFYTSL LLAIGSGGVRGALPALGADQF+QKDPKEAKALATFFN +LLS
Sbjct: 123 PKDCVHGRIAFVFYTSLCLLAIGSGGVRGALPALGADQFNQKDPKEAKALATFFNCLLLS 182

Query: 302 IVIGAAVGVTLIVWVAVNKAWYWGFFTSTVATAVGFIVFAIGKPFYRLHVPGQSPMLRII 361
           +VIGAAVGVTLIVWVAVNKAWYWGFF S +ATAVGFIVFA+GKPFYRLHVPGQSP+LRII
Sbjct: 183 VVIGAAVGVTLIVWVAVNKAWYWGFFISALATAVGFIVFALGKPFYRLHVPGQSPLLRII 242

Query: 362 QVIVVAIKNRGLALPETPTELYEISDKCYTHSIHSKIAHTNQLRFLDKAAILGKEREAEA 421
           QVIVVAIKNRGLALP TP+ELYE+SDK Y  SI SKIA T QLRFLDKA+IL K+ E + 
Sbjct: 243 QVIVVAIKNRGLALPNTPSELYELSDKYYMDSIESKIARTYQLRFLDKASILPKDVEPQP 302

Query: 422 WKVCSVTQVEEVKIITRMLPIFFCTIIMNTCLAQLQTFSVEQGNTRIMDKSLGHLQFPAP 481
           WKVCSVTQVEEVKIITRMLPIF  TIIMNTCLAQLQTFSVEQGNT+IMDKSLGHLQFPAP
Sbjct: 303 WKVCSVTQVEEVKIITRMLPIFISTIIMNTCLAQLQTFSVEQGNTKIMDKSLGHLQFPAP 362

Query: 482 SIPVIPLLFMTFLIPLYEFVFVPFARRITHHPSGITQLQRIGVGLVLSAISMAVAGLVEV 541
           SIPVIPL+FM FLIPLYEFVFVPFAR+ITHHPSGITQLQR+GVGLVLSA+SMAVAGLVE+
Sbjct: 363 SIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAVSMAVAGLVEM 422

Query: 542 KRRHQATTHPDKPMSLFWLSFQYGIFGVADMFTLVGLLEFFYKEAPIGMRSLSTSLTWLS 601
           KRRHQAT HPD+PMSLFWLSFQYGIFG+ADMFTLVGLLEFFYKEAP+GMRSLSTS TWLS
Sbjct: 423 KRRHQATKHPDEPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLS 482

Query: 602 LSLGYYLSSIFVNVVNKVTRKITPSKKGWVEGLIPEDLNHNNLNLFYWFLAILSVLNFLH 661
           LS GYYLSSIFVNV+NKVTRK++PSKKGWVEGLIPEDLN+NNLNLFYWFLAILS+LNF H
Sbjct: 483 LSFGYYLSSIFVNVINKVTRKVSPSKKGWVEGLIPEDLNYNNLNLFYWFLAILSLLNFFH 542

Query: 662 YLYWASWYKYKTEEPIAELNGEGGG-GEGEEGVPVLREEEGSGVDVKAHRSEEKE 716
           YLYWASWYKYKTEEPI +LN  GGG  E EE VP+L EE   G  +K H SEEKE
Sbjct: 543 YLYWASWYKYKTEEPIVKLNDGGGGEREREESVPILHEERSGGA-IKCHHSEEKE 596

BLAST of Lag0006460 vs. ExPASy TrEMBL
Match: A0A6J1DQ47 (LOW QUALITY PROTEIN: protein NRT1/ PTR FAMILY 4.5-like OS=Momordica charantia OX=3673 GN=LOC111023287 PE=3 SV=1)

HSP 1 Score: 998.8 bits (2581), Expect = 1.2e-287
Identity = 517/627 (82.46%), Postives = 556/627 (88.68%), Query Frame = 0

Query: 122 GTEGREIKVVDPRDEGKGGFRATMFIFALLTFESMGFVANGVSLVQYFLLVLHFDLQTAA 181
           G+E REIK VDP ++GKGGFRAT+FIFALL+FE+MGFVAN VSLVQYFLLV+HFDLQTAA
Sbjct: 71  GSEAREIKDVDPSNKGKGGFRATIFIFALLSFENMGFVANMVSLVQYFLLVMHFDLQTAA 130

Query: 182 NTLTNFFGSAFLLSLLGGFLSDTYINRLTTCLVFGVLEILALVMVTVQAYSHDLLPKPDC 241
           NTLTNF GSAFLLSLLGGFLSDTYINRLTT L+FGVLEILALVM+TVQAYS DLLPKPDC
Sbjct: 131 NTLTNFLGSAFLLSLLGGFLSDTYINRLTTSLIFGVLEILALVMITVQAYSQDLLPKPDC 190

Query: 242 PKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFDQKDPKEAKALATFFNYMLLS 301
            KDCVRGRIAFVFYTSLYLLA+GSGGVRGALPALGA QFDQKDP+EAKAL TFFN MLLS
Sbjct: 191 LKDCVRGRIAFVFYTSLYLLAVGSGGVRGALPALGAXQFDQKDPREAKALPTFFNCMLLS 250

Query: 302 IVIGAAVGVTLIVWVAVNKAWYWGFFTSTVATAVGFIVFAIGKPFYRLHVPGQSPMLRII 361
           +V+GAAVGVT+IVWVAVNKAW+WGF  S VATAVGF+VFA+GKPFYRLHVPG+SP+ RII
Sbjct: 251 VVVGAAVGVTVIVWVAVNKAWFWGFLISAVATAVGFVVFAVGKPFYRLHVPGESPVSRII 310

Query: 362 QVIVVAIKNRGLALPETPTELYEISDKCYTHSIHSKIAHTNQLRFLDKAAILGKEREAEA 421
           QVIVVAI+NR L LP++P+ELYEISDK Y  SI SKIAHTNQLRFLDKAAIL  + EA+A
Sbjct: 311 QVIVVAIRNRRLPLPDSPSELYEISDKYYLDSIDSKIAHTNQLRFLDKAAILPNDSEAQA 370

Query: 422 WKVCSVTQVEEVKIITRMLPIFFCTIIMNTCLAQLQTFSVEQGNTRIMDKSLGHLQFPAP 481
           WKVCSVTQVEEVKIITRM+PIF  TIIMNTCLAQLQTFSVEQGNT IMD+SLGHLQFPAP
Sbjct: 371 WKVCSVTQVEEVKIITRMVPIFISTIIMNTCLAQLQTFSVEQGNTTIMDRSLGHLQFPAP 430

Query: 482 SIPVIPLLFMTFLIPLYEFVFVPFARRITHHPSGITQLQRIGVGLVLSAISMAVAGLVEV 541
           SIPVIPL+FM FLIPLYEFVFVPFAR+ITHHPSGITQLQR+GVGLVLSAISMAVAGLVEV
Sbjct: 431 SIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAISMAVAGLVEV 490

Query: 542 KRRHQATTHPDKPMSLFWLSFQYGIFGVADMFTLVGLLEFFYKEAPIGMRSLSTSLTWLS 601
           KRR+QAT HP++PMSLFWLSFQYGIFG+ADMFTLVGLLEFFYKEAP+GMRSLSTS TWLS
Sbjct: 491 KRRNQATNHPNQPMSLFWLSFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTWLS 550

Query: 602 LSLGYYLSSIFVNVVNKVTRKITPSKKGWVEGLIPEDLNHNNLNLFYWFLAILSVLNFLH 661
           LS GYYLSSIFVNVVNKVTR I PSKKGWVEG IPEDLNHNNLNLFYWFLAILS+LNF H
Sbjct: 551 LSFGYYLSSIFVNVVNKVTRNIAPSKKGWVEGQIPEDLNHNNLNLFYWFLAILSILNFFH 610

Query: 662 YLYWASWYKYKTEEPIAEL-----------------NGEGGG-------------GEGEE 718
           YLYWASWYKYKTEEPIAEL                 NG GGG             GEGEE
Sbjct: 611 YLYWASWYKYKTEEPIAELDGGGGEDAPIVQDNRASNGGGGGEEGASIIQDNRAEGEGEE 670

BLAST of Lag0006460 vs. ExPASy TrEMBL
Match: A0A1S4E0E0 (protein NRT1/ PTR FAMILY 4.5-like OS=Cucumis melo OX=3656 GN=LOC103493841 PE=3 SV=1)

HSP 1 Score: 976.9 bits (2524), Expect = 4.7e-281
Identity = 503/664 (75.75%), Postives = 548/664 (82.53%), Query Frame = 0

Query: 122 GTEGREIKVVDPRDEGKGGFRATMFIFALLTFESMGFVANGVSLVQYFLLVLHFDLQTAA 181
           GTE ++IKVVDP+DEGKGGFRATMFIF LLTFESMGFVAN  SLVQYFL V+HFDL+TAA
Sbjct: 17  GTEEKDIKVVDPKDEGKGGFRATMFIFGLLTFESMGFVANMTSLVQYFLFVMHFDLETAA 76

Query: 182 NTLTNFFGSAFLLSLLGGFLSDTYINRLTTCLVFGVLEILALVMVTVQAYSHDLLPKPDC 241
           NTLTNF GSAFLLSLLGGFLSDTYINRLTTCL+FG LE++AL+++TVQAYSHDLLP P C
Sbjct: 77  NTLTNFMGSAFLLSLLGGFLSDTYINRLTTCLIFGFLEVIALILITVQAYSHDLLPSPLC 136

Query: 242 PKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFDQKDPKEAKALATFFNYMLLS 301
           PKDCV+GRIAFVFYTSLYLLAIGSGGVRGALPALGADQF+QKDPKEAKAL TFFNYMLLS
Sbjct: 137 PKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALGTFFNYMLLS 196

Query: 302 IVIGAAVGVTLIVWVAVNKAWYWGFFTSTVATAVGFIVFAIGKPFYRLHVPGQSPMLRII 361
           +V+GAAVGVT+IVWVAVNKAWYWGFF S +A  VGFI+FAIGKPFYR+ VPGQSP+LR+I
Sbjct: 197 VVLGAAVGVTVIVWVAVNKAWYWGFFISALAALVGFIIFAIGKPFYRIQVPGQSPILRVI 256

Query: 362 QVIVVAIKNRGLALPETPTELYEISDKCYTHSIHSKIAHTNQLRFLDKAAILGKEREAEA 421
           QVIVVAIKNR L LP+TP ELYEISDK Y  SIH KI HTNQLRFLDKAAI+ K+ E + 
Sbjct: 257 QVIVVAIKNRRLRLPDTPNELYEISDKVYMDSIHYKIVHTNQLRFLDKAAIVPKDIEPQP 316

Query: 422 WKVCSVTQVEEVKIITRMLPIFFCTIIMNTCLAQLQTFSVEQGNTRIMDKSLGHLQFPAP 481
           W VCSVTQVEEVKIITRM+PIF  TIIMNTCLAQLQTFSVEQGNT IMDKSLGH QFPAP
Sbjct: 317 WNVCSVTQVEEVKIITRMVPIFISTIIMNTCLAQLQTFSVEQGNTIIMDKSLGHFQFPAP 376

Query: 482 SIPVIPLLFMTFLIPLYEFVFVPFARRITHHPSGITQLQRIGVGLVLSAISMAVAGLVEV 541
           SIPVIPL+FM FLIPLYEFVFVPFAR+ITHHPSGITQLQR+GVGLVLSAI+MAVAGLVEV
Sbjct: 377 SIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAIAMAVAGLVEV 436

Query: 542 KRRHQATTHPDKPMSLFWLSFQYGIFGVADMFTLVGLLEFFYKEAPIGMRSLSTSLTWLS 601
           KRRHQA  HPD+P+SLFWL+FQYGIFG+ADMFTLVGLLEFFYKEAP+GMRSLSTS T+LS
Sbjct: 437 KRRHQAIKHPDEPISLFWLAFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLS 496

Query: 602 LSLGYYLSSIFVNVVNKVTRKITPSKKGWVEGLIPEDLNHNNLNLFYWFLAILSVLNFLH 661
           L+LGYYLSSIFVN+VNKVTR ITPSK+GWVEGLIPEDLNHNNLNLFYWFLAILSVLNF H
Sbjct: 497 LALGYYLSSIFVNIVNKVTRNITPSKRGWVEGLIPEDLNHNNLNLFYWFLAILSVLNFFH 556

Query: 662 YLYWASWYKYKTEEPIAELNGEG------------------------------------- 718
           YLYWASWYKYKTEEPI ELN +G                                     
Sbjct: 557 YLYWASWYKYKTEEPIEELNSKGGETTKEPIAELNSKEGETMTKEPIAELNKQGEEIKEP 616

BLAST of Lag0006460 vs. ExPASy TrEMBL
Match: A0A5A7ULM1 (Protein NRT1/ PTR FAMILY 4.5-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold323G00540 PE=3 SV=1)

HSP 1 Score: 976.5 bits (2523), Expect = 6.1e-281
Identity = 503/664 (75.75%), Postives = 547/664 (82.38%), Query Frame = 0

Query: 122 GTEGREIKVVDPRDEGKGGFRATMFIFALLTFESMGFVANGVSLVQYFLLVLHFDLQTAA 181
           GTE ++IKVVDP+DEGKGGFRATMFIF LLTFESMGFVAN  SLVQYFL V+HFDL+TAA
Sbjct: 2   GTEEKDIKVVDPKDEGKGGFRATMFIFGLLTFESMGFVANMTSLVQYFLFVMHFDLETAA 61

Query: 182 NTLTNFFGSAFLLSLLGGFLSDTYINRLTTCLVFGVLEILALVMVTVQAYSHDLLPKPDC 241
           NTLTNF GSAFLLSLLGGFLSDTYINRLTTCL+FG LE++AL+++TVQAYSHDLLP P C
Sbjct: 62  NTLTNFMGSAFLLSLLGGFLSDTYINRLTTCLIFGFLEVIALILITVQAYSHDLLPSPLC 121

Query: 242 PKDCVRGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFDQKDPKEAKALATFFNYMLLS 301
           PKDCV+GRIAFVFYTSLYLLAIGSGGVRGALPALGADQF+QKDPKEAKAL TFFNYMLLS
Sbjct: 122 PKDCVKGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFNQKDPKEAKALGTFFNYMLLS 181

Query: 302 IVIGAAVGVTLIVWVAVNKAWYWGFFTSTVATAVGFIVFAIGKPFYRLHVPGQSPMLRII 361
           +V+GAAVGVT+IVWVAVNKAWYWGFF S +A  VGFI+FAIGKPFYR+ VPGQSP+LR+I
Sbjct: 182 VVLGAAVGVTVIVWVAVNKAWYWGFFISALAALVGFIIFAIGKPFYRIQVPGQSPILRVI 241

Query: 362 QVIVVAIKNRGLALPETPTELYEISDKCYTHSIHSKIAHTNQLRFLDKAAILGKEREAEA 421
           QVIVVAIKNR L LP+TP ELYEISDK Y  SIH KI HTNQLRFLDKAAI+ K+ E + 
Sbjct: 242 QVIVVAIKNRRLRLPDTPNELYEISDKVYMDSIHYKIVHTNQLRFLDKAAIVPKDIEPQP 301

Query: 422 WKVCSVTQVEEVKIITRMLPIFFCTIIMNTCLAQLQTFSVEQGNTRIMDKSLGHLQFPAP 481
           W VCSVTQVEEVKIITRM+PIF  TIIMNTCLAQLQTFSVEQGNT IMDKSLGH QFPAP
Sbjct: 302 WNVCSVTQVEEVKIITRMVPIFISTIIMNTCLAQLQTFSVEQGNTIIMDKSLGHFQFPAP 361

Query: 482 SIPVIPLLFMTFLIPLYEFVFVPFARRITHHPSGITQLQRIGVGLVLSAISMAVAGLVEV 541
           SIPVIPL+FM FLIPLYEFVFVPFAR+ITHHPSGITQLQR+GVGLVLSAI+MAVAGLVEV
Sbjct: 362 SIPVIPLVFMAFLIPLYEFVFVPFARKITHHPSGITQLQRVGVGLVLSAIAMAVAGLVEV 421

Query: 542 KRRHQATTHPDKPMSLFWLSFQYGIFGVADMFTLVGLLEFFYKEAPIGMRSLSTSLTWLS 601
           KRRHQA  HPD+P+SLFWL+FQYGIFG+ADMFTLVGLLEFFYKEAP+GMRSLSTS T+LS
Sbjct: 422 KRRHQAIKHPDEPISLFWLAFQYGIFGIADMFTLVGLLEFFYKEAPVGMRSLSTSFTFLS 481

Query: 602 LSLGYYLSSIFVNVVNKVTRKITPSKKGWVEGLIPEDLNHNNLNLFYWFLAILSVLNFLH 661
           L+LGYYLSSIFVN+VNKVTR ITPSK+GWVEGLIPEDLNHNNLNLFYWFLAILSVLNF H
Sbjct: 482 LALGYYLSSIFVNIVNKVTRNITPSKRGWVEGLIPEDLNHNNLNLFYWFLAILSVLNFFH 541

Query: 662 YLYWASWYKYKTEEPIAELNGEG------------------------------------- 718
           YLYWASWYKYKTEEPI ELN  G                                     
Sbjct: 542 YLYWASWYKYKTEEPIEELNSRGGETTKEPIAELNSKEGETMTKEPIAELNKQGEEIKEP 601

BLAST of Lag0006460 vs. TAIR 10
Match: AT1G27040.2 (Major facilitator superfamily protein )

HSP 1 Score: 418.7 bits (1075), Expect = 9.5e-117
Identity = 236/550 (42.91%), Postives = 341/550 (62.00%), Query Frame = 0

Query: 139 GGFRATMFIFALLTFESMGFVANGVSLVQYFLLVLHFDLQTAANTLTNFFGSAFLLSLLG 198
           GG  A  F+ A+   E++ F+AN  +LV Y    +H  L  +++ +T F  +AFLL+LLG
Sbjct: 25  GGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEVTTFMATAFLLALLG 84

Query: 199 GFLSDTYINRLTTCLVFGVLEILALVMVTVQAYSHDLLPKPDCPK------DCVRGRIAF 258
           GFL+D + +     L+   +E L L+++T+QA    L+P P C        + V G  A 
Sbjct: 85  GFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMP-PPCKSSAALRCEVVGGSKAA 144

Query: 259 VFYTSLYLLAIGSGGVRGALPALGADQFDQKDPKEAKALATFFNYMLLSIVIGAAVGVTL 318
             +  LYL+++G GG++G+LP+ GA+QFD+  PK  K  +TFFNY +  +  GA V VT 
Sbjct: 145 FLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAVTF 204

Query: 319 IVWVAVNKAWYWGFFTSTVATAVGFIVFAIGKPFYRLHVPGQSPMLRIIQVI----VVAI 378
           +VW+  NK W WGF  ST++  +  +VF +G  FY+  +P  SP+  I +V+    +V+ 
Sbjct: 205 VVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSPLTTIFKVLLAASIVSC 264

Query: 379 KNRGLALPETPTELY-EISDKCYTHSIHSKIAHTNQLRFLDKAAILGKEREAEAWKVCSV 438
            ++  +   T  E+  E  +K  + S+      TN L  L+K AI GK      W  C+V
Sbjct: 265 SSKTSSNHFTSREVQSEHEEKTPSQSL------TNSLTCLNK-AIEGKTH--HIWLECTV 324

Query: 439 TQVEEVKIITRMLPIFFCTIIMNTCLAQLQTFSVEQGNTRIMDKSLGHLQFPAPSIPVIP 498
            QVE+VKI+ +MLPIF CTI++N CLAQL T+SV Q  T  M++ + +   P+ S+PV P
Sbjct: 325 QQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAAT--MNRKIVNFNVPSASLPVFP 384

Query: 499 LLFMTFLIPLYEFVFVPFARRITHHPSGITQLQRIGVGLVLSAISMAVAGLVEVKRRHQA 558
           ++FM  L P Y+ + +PFAR++T    GIT LQRIGVGLVLS ++MAVA LVE+KR+  A
Sbjct: 385 VVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVA 444

Query: 559 ------TTHPDKPMSLFWLSFQYGIFGVADMFTLVGLLEFFYKEAPIGMRSLSTSLTWLS 618
                  +    P++  W++ QY   G AD+FTL GLLEFF+ EAP  MRSL+TSL+W S
Sbjct: 445 REAGLLDSEETLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWAS 504

Query: 619 LSLGYYLSSIFVNVVNKVTRKITPSKKGWVEGLIPEDLNHNNLNLFYWFLAILSVLNFLH 672
           L+LGYYLSS+ V +VN+VT+  +  +  W    + E LN N L+LFYW + +LSV+NFLH
Sbjct: 505 LALGYYLSSVMVPIVNRVTK--SAGQSPW----LGEKLNRNRLDLFYWLMCVLSVVNFLH 556

BLAST of Lag0006460 vs. TAIR 10
Match: AT1G27040.1 (Major facilitator superfamily protein )

HSP 1 Score: 418.7 bits (1075), Expect = 9.5e-117
Identity = 236/550 (42.91%), Postives = 341/550 (62.00%), Query Frame = 0

Query: 139 GGFRATMFIFALLTFESMGFVANGVSLVQYFLLVLHFDLQTAANTLTNFFGSAFLLSLLG 198
           GG  A  F+ A+   E++ F+AN  +LV Y    +H  L  +++ +T F  +AFLL+LLG
Sbjct: 29  GGMLAASFVLAVEILENLAFLANASNLVLYLKNFMHMSLARSSSEVTTFMATAFLLALLG 88

Query: 199 GFLSDTYINRLTTCLVFGVLEILALVMVTVQAYSHDLLPKPDCPK------DCVRGRIAF 258
           GFL+D + +     L+   +E L L+++T+QA    L+P P C        + V G  A 
Sbjct: 89  GFLADAFFSTFVIFLISASIEFLGLILLTIQARRPSLMP-PPCKSSAALRCEVVGGSKAA 148

Query: 259 VFYTSLYLLAIGSGGVRGALPALGADQFDQKDPKEAKALATFFNYMLLSIVIGAAVGVTL 318
             +  LYL+++G GG++G+LP+ GA+QFD+  PK  K  +TFFNY +  +  GA V VT 
Sbjct: 149 FLFVGLYLVSLGIGGIKGSLPSHGAEQFDEGTPKGRKQRSTFFNYYVFCLSCGALVAVTF 208

Query: 319 IVWVAVNKAWYWGFFTSTVATAVGFIVFAIGKPFYRLHVPGQSPMLRIIQVI----VVAI 378
           +VW+  NK W WGF  ST++  +  +VF +G  FY+  +P  SP+  I +V+    +V+ 
Sbjct: 209 VVWIEDNKGWEWGFGVSTISIFLSILVFLLGSRFYKNKIPRGSPLTTIFKVLLAASIVSC 268

Query: 379 KNRGLALPETPTELY-EISDKCYTHSIHSKIAHTNQLRFLDKAAILGKEREAEAWKVCSV 438
            ++  +   T  E+  E  +K  + S+      TN L  L+K AI GK      W  C+V
Sbjct: 269 SSKTSSNHFTSREVQSEHEEKTPSQSL------TNSLTCLNK-AIEGKTH--HIWLECTV 328

Query: 439 TQVEEVKIITRMLPIFFCTIIMNTCLAQLQTFSVEQGNTRIMDKSLGHLQFPAPSIPVIP 498
            QVE+VKI+ +MLPIF CTI++N CLAQL T+SV Q  T  M++ + +   P+ S+PV P
Sbjct: 329 QQVEDVKIVLKMLPIFGCTIMLNCCLAQLSTYSVHQAAT--MNRKIVNFNVPSASLPVFP 388

Query: 499 LLFMTFLIPLYEFVFVPFARRITHHPSGITQLQRIGVGLVLSAISMAVAGLVEVKRRHQA 558
           ++FM  L P Y+ + +PFAR++T    GIT LQRIGVGLVLS ++MAVA LVE+KR+  A
Sbjct: 389 VVFMLILAPTYDHLIIPFARKVTKSEIGITHLQRIGVGLVLSIVAMAVAALVELKRKQVA 448

Query: 559 ------TTHPDKPMSLFWLSFQYGIFGVADMFTLVGLLEFFYKEAPIGMRSLSTSLTWLS 618
                  +    P++  W++ QY   G AD+FTL GLLEFF+ EAP  MRSL+TSL+W S
Sbjct: 449 REAGLLDSEETLPITFLWIALQYLFLGSADLFTLAGLLEFFFTEAPSSMRSLATSLSWAS 508

Query: 619 LSLGYYLSSIFVNVVNKVTRKITPSKKGWVEGLIPEDLNHNNLNLFYWFLAILSVLNFLH 672
           L+LGYYLSS+ V +VN+VT+  +  +  W    + E LN N L+LFYW + +LSV+NFLH
Sbjct: 509 LALGYYLSSVMVPIVNRVTK--SAGQSPW----LGEKLNRNRLDLFYWLMCVLSVVNFLH 560

BLAST of Lag0006460 vs. TAIR 10
Match: AT1G69850.1 (nitrate transporter 1:2 )

HSP 1 Score: 409.1 bits (1050), Expect = 7.5e-114
Identity = 227/565 (40.18%), Postives = 338/565 (59.82%), Query Frame = 0

Query: 139 GGFRATMFIFALLTFESMGFVANGVSLVQYFLLVLHFDLQTAANTLTNFFGSAFLLSLLG 198
           GG  A  F+  +   E++ ++AN  +LV Y    +H     +AN +TNF G+AFLL+LLG
Sbjct: 27  GGMLAASFVLVVEILENLAYLANASNLVLYLREYMHMSPSKSANDVTNFMGTAFLLALLG 86

Query: 199 GFLSDTYINRLTTCLVFGVLEILALVMVTVQAYSHDLLPKPDCPK---DCVRGRIAFVFY 258
           GFLSD + +     L+   +E L L+++T+QA +  L+P P C     + V G  A + +
Sbjct: 87  GFLSDAFFSTFQIFLISASIEFLGLIILTIQARTPSLMP-PSCDSPTCEEVSGSKAAMLF 146

Query: 259 TSLYLLAIGSGGVRGALPALGADQFDQKDPKEAKALATFFNYMLLSIVIGAAVGVTLIVW 318
             LYL+A+G GG++G+L + GA+QFD+  PK  K  +TFFNY +  +  GA V VT +VW
Sbjct: 147 VGLYLVALGVGGIKGSLASHGAEQFDESTPKGRKQRSTFFNYFVFCLACGALVAVTFVVW 206

Query: 319 VAVNKAWYWGFFTSTVATAVGFIVFAIGKPFYRLHVPGQSPMLRIIQVIVVA-------- 378
           +  NK W WGF  ST+A  V  ++F  G  FYR  +P  SP+  I++V++ A        
Sbjct: 207 LEDNKGWEWGFGVSTIAIFVSILIFLSGSRFYRNKIPCGSPLTTILKVLLAASVKCCSSG 266

Query: 379 -IKNRGLALPETPT------------ELYEISDKCYTHSIHSKIAHTNQLRFLDKAAILG 438
              N   ++  +P+               E+       ++  +   TN L+ L+ AA   
Sbjct: 267 SSSNAVASMSVSPSNHCVSKGKKEVESQGELEKPRQEEALPPRAQLTNSLKVLNGAA--- 326

Query: 439 KEREAEAWKVCSVTQVEEVKIITRMLPIFFCTIIMNTCLAQLQTFSVEQGNTRIMDKSLG 498
            E+       C+V QVE+VKI+ +MLPIF CTI++N CLAQL TFSV+Q  +  M+  +G
Sbjct: 327 DEKPVHRLLECTVQQVEDVKIVLKMLPIFACTIMLNCCLAQLSTFSVQQAAS--MNTKIG 386

Query: 499 HLQFPAPSIPVIPLLFMTFLIPLYEFVFVPFARRITHHPSGITQLQRIGVGLVLSAISMA 558
            L+ P  S+P+ P++F+  L P+Y+ + +PFAR+ T   +G+T LQRIGVGLVLS ++MA
Sbjct: 387 SLKIPPASLPIFPVVFIMILAPIYDHLIIPFARKATKTETGVTHLQRIGVGLVLSILAMA 446

Query: 559 VAGLVEVKRRHQA------TTHPDKPMSLFWLSFQYGIFGVADMFTLVGLLEFFYKEAPI 618
           VA LVE+KR+  A       +    P++  W++ QY   G AD+FTL GLLE+F+ EAP 
Sbjct: 447 VAALVEIKRKGVAKDSGLLDSKETLPVTFLWIALQYLFLGSADLFTLAGLLEYFFTEAPS 506

Query: 619 GMRSLSTSLTWLSLSLGYYLSSIFVNVVNKVTRKITPSKKGWVEGLIPEDLNHNNLNLFY 674
            MRSL+TSL+W SL++GYYLSS+ V++VN +T   +     W+ G   + +N   L+ FY
Sbjct: 507 SMRSLATSLSWASLAMGYYLSSVIVSIVNSITG--SSGNTPWLRG---KSINRYKLDYFY 566

BLAST of Lag0006460 vs. TAIR 10
Match: AT1G59740.1 (Major facilitator superfamily protein )

HSP 1 Score: 398.7 bits (1023), Expect = 1.0e-110
Identity = 229/540 (42.41%), Postives = 328/540 (60.74%), Query Frame = 0

Query: 139 GGFRATMFIFALLTFESMGFVANGVSLVQYFLLVLHFDLQTAANTLTNFFGSAFLLSLLG 198
           GG RA +F+  L  FE MG  A G +L+ Y +  +HF L  AAN +TNF G+ F+ +LLG
Sbjct: 43  GGMRAALFVLGLQAFEIMGIAAVGNNLITYVINEMHFPLSKAANIVTNFVGTIFIFALLG 102

Query: 199 GFLSDTYINRLTTCLVFGVLEILALVMVTVQAYSHDLLP---KPDCPKDC--VRGRIAFV 258
           G+LSD ++    T ++FG +E+   ++++VQA+   L P    P   + C   +G  A +
Sbjct: 103 GYLSDAFLGSFWTIIIFGFVELSGFILLSVQAHLPQLKPPKCNPLIDQTCEEAKGFKAMI 162

Query: 259 FYTSLYLLAIGSGGVRGALPALGADQFDQKDPKEAKALATFFNYMLLSIVIGAAVGVTLI 318
           F+ +LYL+A+GSG V+  + A GADQF Q  PK++K L+++FN    +  +G  + +TL+
Sbjct: 163 FFMALYLVALGSGCVKPNMIAHGADQFSQSHPKQSKRLSSYFNAAYFAFSMGELIALTLL 222

Query: 319 VWVAVNKAWYWGFFTSTVATAVGFIVFAIGKPFYRLHVPGQSPMLRIIQVIVVAIKNRGL 378
           VWV  +     GF  S  A  +G I    G  ++R   P +S    I  VIV AI  R L
Sbjct: 223 VWVQTHSGMDIGFGVSAAAMTMGIISLVSGTMYFRNKRPRRSIFTPIAHVIVAAILKRKL 282

Query: 379 ALPETPTELYEISDKCYTHSIHSKIAHTNQLRFLDKAAILGKEREAE--AWKVCSVTQVE 438
           A P  P  L+            S + HT + RFLDKA I  ++   +   W++C+VTQVE
Sbjct: 283 ASPSDPRMLHGDHHVANDVVPSSTLPHTPRFRFLDKACIKIQDTNTKESPWRLCTVTQVE 342

Query: 439 EVKIITRMLPIFFCTIIMNTCLAQLQTFSVEQG---NTRIMDKSLGHLQFPAPSIPVIPL 498
           +VK +  ++PIF  TI+ NT LAQLQTFSV+QG   NTR+ +        P  S+  IP 
Sbjct: 343 QVKTLISLVPIFASTIVFNTILAQLQTFSVQQGSSMNTRLSNS----FHIPPASLQAIPY 402

Query: 499 LFMTFLIPLYEFVFVPFARRITHHPSGITQLQRIGVGLVLSAISMAVAGLVEVKRRHQAT 558
           + + FL+PLY+   VPFAR++T H SGI  L RIG+GL LS  SM  A ++E KRR  ++
Sbjct: 403 IMLIFLVPLYDSFLVPFARKLTGHNSGIPPLTRIGIGLFLSTFSMVSAAMLEKKRR-DSS 462

Query: 559 THPDKPMSLFWLSFQYGIFGVADMFTLVGLLEFFYKEAPIGMRSLSTSLTWLSLSLGYYL 618
               + +S+FW++ Q+ IFG+++MFT VGL+EFFYK++  GM S   +LT+ S S G+Y 
Sbjct: 463 VLDGRILSIFWITPQFLIFGISEMFTAVGLIEFFYKQSAKGMESFLMALTYCSYSFGFYF 522

Query: 619 SSIFVNVVNKVTRKITPSKKGWVEGLIPEDLNHNNLNLFYWFLAILSVLNFLHYLYWASW 669
           SS+ V+VVNK+T     S KGW   L   DLN + L+LFYW LA+LS+LNFL YL+W+ W
Sbjct: 523 SSVLVSVVNKITSTSVDS-KGW---LGENDLNKDRLDLFYWLLAVLSLLNFLSYLFWSRW 573

BLAST of Lag0006460 vs. TAIR 10
Match: AT1G33440.1 (Major facilitator superfamily protein )

HSP 1 Score: 387.9 bits (995), Expect = 1.8e-107
Identity = 234/549 (42.62%), Postives = 329/549 (59.93%), Query Frame = 0

Query: 134 RDEGKGGFRATMFIFALLTFESMGFVANGVSLVQYFLLVLHFDLQTAANTLTNFFGSAFL 193
           R    GG RA +F+     FE M   A G +L+ Y    +HF L  +AN +TNF G+ FL
Sbjct: 35  RPSKHGGTRAALFVLGFQAFEMMAIAAVGNNLITYVFNEMHFPLSKSANLVTNFIGTVFL 94

Query: 194 LSLLGGFLSDTYINRLTTCLVFGVLEILALVMVTVQAYSHDLLPKPDCPK-----DCV-- 253
           LSLLGGFLSD+Y+    T LVFGV+EI   ++++VQA+  +L P P+C        CV  
Sbjct: 95  LSLLGGFLSDSYLGSFRTMLVFGVIEISGFILLSVQAHLPELRP-PECNMKSTTIHCVEA 154

Query: 254 RGRIAFVFYTSLYLLAIGSGGVRGALPALGADQFDQKDPKEAKALATFFNYMLLSIVIGA 313
            G  A   YT+L L+A+GSG ++  + + GA+QF +KD    + L++FFN    +  +G 
Sbjct: 155 NGYKAATLYTALCLVALGSGCLKPNIISHGANQFQRKD---LRKLSSFFNAAYFAFSMGQ 214

Query: 314 AVGVTLIVWVAVNKAWYWGFFTSTVATAVGFIVFAIGKPFYRLHVPGQSPMLRIIQVIVV 373
            + +TL+VWV  +     GF  S    A G I    G  FYR   P  S    I QV V 
Sbjct: 215 LIALTLLVWVQTHSGMDVGFGVSAAVMAAGMISLVAGTSFYRNKPPSGSIFTPIAQVFVA 274

Query: 374 AIKNRGLALPETPTELYEISDKCYTHSIHSK-IAHTNQLRFLDKAAILGKEREAEA-WKV 433
           AI  R    P  P  +++ S    T  +  K + H+N+ RFLDKA I  + +  E+ W++
Sbjct: 275 AITKRKQICPSNPNMVHQPS----TDLVRVKPLLHSNKFRFLDKACIKTQGKAMESPWRL 334

Query: 434 CSVTQVEEVKIITRMLPIFFCTIIMNTCLAQLQTFSVEQG---NTRIMDKSLGHLQFPAP 493
           C++ QV +VKI+  ++PIF CTII NT LAQLQTFSV+QG   NT I        Q P  
Sbjct: 335 CTIEQVHQVKILLSVIPIFACTIIFNTILAQLQTFSVQQGSSMNTHITKT----FQIPPA 394

Query: 494 SIPVIPLLFMTFLIPLYEFVFVPFARRITHHPSGITQLQRIGVGLVLSAISMAVAGLVEV 553
           S+  IP + + F +PLYE  FVP AR++T + SGI+ LQRIG GL L+  SM  A LVE 
Sbjct: 395 SLQAIPYIILIFFVPLYETFFVPLARKLTGNDSGISPLQRIGTGLFLATFSMVAAALVEK 454

Query: 554 KRRHQATTHPDKPMSLFWLSFQYGIFGVADMFTLVGLLEFFYKEAPIGMRSLSTSLTWLS 613
           KRR ++    +  +S+FW++ Q+ IFG+++MFT VGL+EFFYK++   M+S  T++T+ S
Sbjct: 455 KRR-ESFLEQNVMLSIFWIAPQFLIFGLSEMFTAVGLVEFFYKQSSQSMQSFLTAMTYCS 514

Query: 614 LSLGYYLSSIFVNVVNKVTRKI-TPSKKGWVEGLIPEDLNHNNLNLFYWFLAILSVLNFL 670
            S G+YLSS+ V+ VN+VT    + +K+GW   L   DLN + L+ FYW LA LS +NF 
Sbjct: 515 YSFGFYLSSVLVSTVNRVTSSNGSGTKEGW---LGDNDLNKDRLDHFYWLLASLSFINFF 567