Lag0006427 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0006427
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	2,3-bisphosphoglycerate-independent phosphoglycerate mutase
Location	chr6: 42353355 .. 42356452 (+)
RNA-Seq Expression	Lag0006427
Synteny	Lag0006427

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTACTTCCCAACAGCCTAAGAAAAGAGTGGCGTTTGTGCTTATCGATGGGTTGGGTGATGTCTCATTACCAAAATTTGGATTTAAGACTCCTTTGCAAGTTGCCAAAGTTCCTAACTTGGATGCGATTGCTTCTGCTGGTGTTAATGGTCTCATGGACCCTGTTGAAGTAGGATTGGGTTGTGGAAGTGACACAGCTCATCTTTCACTTTTGGGCTATGACCCTAGAGTATATTATAGGGGTCGAGGTGCATTTGAGTCCATGGGAGCTGGATTGGCTATGTCACCTGGAGACATTGCGTTTAAGGTGTATTTCTCTATTCTTTTTCCCCTAAATAAAATTCTTCCCATTTTTTTTAATCATTTATTACTGGCTCTCGCAACTAGAATGCATAAGTTTGTTGGTCTATGTTTTTAGTTTAATAATGAAAAGAGAAGGCGATTATTGGTTTAGCTGAATGTTTTCTCTACAAGTTTAGGGAGACTGAACTTAACGAGTGATGGAATATTTATGCAAATATGATTTGATCAGGTAAACCCTGTTATTTGGCATGTAAACAAATGCTTTTATTTCTCATGATTTAACGACATAGTGCTTTTAACTTGCTCAGATTTTTTTAAGGAGAAATTTTCTAACTGAATCATATAATTAAATCCAAGCTCCTGTCTCTTTCTCTCCGGTCAATTTCTTTTCTGGAACTGGGACTTTTTGTCATGGTTGCTATATCCATTTCTCTTTTGGTTTGAAGTAGTAGTGTGCTTCTTGTGAACTCCTTCCCGATGGCTGCAGTTTCGACTTTTCTGCACAAAATCATACCTTATCTTCTCCCTGCTTTAATTATTTAATCTTCTCTAATTTTGTAGCTATATTTCTTGGGAAATGTGAAGAGTACCATTATATATTCTTTTTCCCATTTTTAGTCTACAAACTTACAAGTGACTTCACAATCGCAGTCAAACTTTGCTACATTGGACGAGAAAACAGGAATAGTAACCAGTAGGAGGGCTGATAGACACTTTGAGGAGGAAGGACCAATATTATGTGCAGCACTTGATAGAATGAAGATACCAAGTTTCCCAGAGTATGAAGTTAGAGTCAGGTAGTTTGTACTCCTTACATCATTAAAATATCAAATGTTTCTTCCTGTCTGGTCCATTCTATCAGTTTGTAAATAACCGTACTTTATTTGGAAACATTTCATTATATCAACTCATTCGAGTATCATAAAATTGAAATCCAAAGAAAAACTCTAGTTCCAAGCGGTTGTCAGAGAAAGTCCAAATGGATTATAAGCTCATAAGACAAACGATCATTATAATAGACCTAATGACTCTAGTCTTATTTGCTTGAGACTTTTAATTCCAATGCCAGATAATAAGATTTATCTCATCCTCTCTTCTTTTTCCTCCCTACAGGTATGCCACAGAGCATAGATGTGGCGTGGTTGTGAAAGGACCAAATTTGAGCGGGAACATATCGGGAACAGACCCTTTGAAGGACAACCGCTTACTTTTGCAAGCAGAGGCTTTAGATGATTCTGATGAAGCAAAGCTTACTGCAGCTGTTGTTAATGAGTTATCCAAGGAAATCTCGCGAATTCTGGTTTCTCACCCAATCAATGCAAAGCGTGCAGCAGAAGGGAAGAACATAGCCAACATTGTTCTTTTACGAGGTTGCGGCATTCGAATTGAGGTTTGTTCTTTTATATATTTCTCCTATCACCTATGCTATTTGCACTGAGTTTTTATTCATAAGATTGCAATGTTCATGCTTCCTTTTGTTTGGCTGCACCAAGTGTTGATCCCCCTCCCACGAAATGCAAAACAAGATATACTCTGCTCTGGGAATGACGTTTGTAACATTGCCCTTTTACATTTATGAAAAAAATAACTGGTTGCAATGCAGTGTGTAAATCATGCACTTTAAAAATTGTATAATTCGAATAGGGTAGACAACTTGGGATCTTGCATTGAGCTTTTGGCTTTTAATGAGATGCTATTCCATGACAGCAAGTGTATTGATGAACTTCAATGGACCGACTCTTTCTCACAATTTTCATTCTGCATAACAGGTTCCTCCATTTGAGAAGAAACATGGTTTATGGCCATGCATGGTGGCTCCCACTAAAATTATAGCTGGTCTAGGCTTATCACTAGGAATCGATATCCTAGATGCTCCAGGAGCAACTGGAGATTATCGAACACTCTTAACCTCGAAAGCTACTGCAATGGCAAAGGGACTCTCAGCTCCTTTGCAATCTTGTCCCAGTGTGTTTGTGCCCGGAGAAGATGAGCTCAAACAAGGTCGGGCCGATGGTTACAGTTTCGGTTTCCTTCATGTTAAGGTCAGTCCACTTTCTCCTCAACTCTCAAAAACACACCCATTAGTCAATAAATTTTTAGAACCCCTCCTACAAAAGTTCTCATTTCATATGCAACTATTCCAGCCACACGCTTGAAACAAGGTTTTTGGCTATTGAAGAGGATCATCAAATATAGCAACACTCTCCTTTGAATTTTCATTTTTGTAGACTTTTCCTAACAGTTTTGTTTATTTTCAACCGTATTCGAAGTCCGATCTCATGGTGTTGTTGGCTACTGATCTTGTAGGCAATAGATGACGCCGGGCACGATAAGGCAACTATATTCAAAGTTAAGGGAATGGAAGCTGTAGATAGAGCAATAGGACAGCTAGCCAAGCTTCTCTGGAAGGCAGAATCTACTGGAAATTTCCAGTACTACCTCTGTGTGACTGGAGACCACTCAACTCCAGTTGAGTATGGAGATCACAGTTTCGAGCCAGTGCCATTTACAATATGTAGATTGAAAGACTTTGTGGACGCTGTGGGCGGGGAGTCGGTAATTGCAAGCATTTCCCTCGAACCGTTTCCGCTCCCGACGATCGTACCCGGTGAGGACTTGGACTGGAGAGAAATCCAGAAGGTGGAAGAAGAGAGAAGCAAAAACTCCCAAGCTTGTGGTGGTGACTCAGTTTGTGAGTTTGATGAGATTGCAGTTGCCAAGGGATGTCTTGGACGCTTTCCTGGTGGAGAGATGATGGGTATCATAAAGAGATTCCTCCAATTGGATGCTTGA

mRNA sequence

ATGGTTACTTCCCAACAGCCTAAGAAAAGAGTGGCGTTTGTGCTTATCGATGGGTTGGGTGATGTCTCATTACCAAAATTTGGATTTAAGACTCCTTTGCAAGTTGCCAAAGTTCCTAACTTGGATGCGATTGCTTCTGCTGGTGTTAATGGTCTCATGGACCCTGTTGAAGTAGGATTGGGTTGTGGAAGTGACACAGCTCATCTTTCACTTTTGGGCTATGACCCTAGAGTATATTATAGGGGTCGAGGTGCATTTGAGTCCATGGGAGCTGGATTGGCTATGTCACCTGGAGACATTGCGTTTAAGTCAAACTTTGCTACATTGGACGAGAAAACAGGAATAGTAACCAGTAGGAGGGCTGATAGACACTTTGAGGAGGAAGGACCAATATTATGTGCAGCACTTGATAGAATGAAGATACCAAGTTTCCCAGAGTATGAAGTTAGAGTCAGGTATGCCACAGAGCATAGATGTGGCGTGGTTGTGAAAGGACCAAATTTGAGCGGGAACATATCGGGAACAGACCCTTTGAAGGACAACCGCTTACTTTTGCAAGCAGAGGCTTTAGATGATTCTGATGAAGCAAAGCTTACTGCAGCTGTTGTTAATGAGTTATCCAAGGAAATCTCGCGAATTCTGGTTTCTCACCCAATCAATGCAAAGCGTGCAGCAGAAGGGAAGAACATAGCCAACATTGTTCTTTTACGAGGTTGCGGCATTCGAATTGAGGTTCCTCCATTTGAGAAGAAACATGGTTTATGGCCATGCATGGTGGCTCCCACTAAAATTATAGCTGGTCTAGGCTTATCACTAGGAATCGATATCCTAGATGCTCCAGGAGCAACTGGAGATTATCGAACACTCTTAACCTCGAAAGCTACTGCAATGGCAAAGGGACTCTCAGCTCCTTTGCAATCTTGTCCCAGTGTGTTTGTGCCCGGAGAAGATGAGCTCAAACAAGGTCGGGCCGATGGTTACAGTTTCGGTTTCCTTCATGTTAAGGCAATAGATGACGCCGGGCACGATAAGGCAACTATATTCAAAGTTAAGGGAATGGAAGCTGTAGATAGAGCAATAGGACAGCTAGCCAAGCTTCTCTGGAAGGCAGAATCTACTGGAAATTTCCAGTACTACCTCTGTGTGACTGGAGACCACTCAACTCCAGTTGAGTATGGAGATCACAGTTTCGAGCCAGTGCCATTTACAATATGTAGATTGAAAGACTTTGTGGACGCTGTGGGCGGGGAGTCGGTAATTGCAAGCATTTCCCTCGAACCGTTTCCGCTCCCGACGATCGTACCCGGTGAGGACTTGGACTGGAGAGAAATCCAGAAGGTGGAAGAAGAGAGAAGCAAAAACTCCCAAGCTTGTGGTGGTGACTCAGTTTGTGAGTTTGATGAGATTGCAGTTGCCAAGGGATGTCTTGGACGCTTTCCTGGTGGAGAGATGATGGGTATCATAAAGAGATTCCTCCAATTGGATGCTTGA

Coding sequence (CDS)

ATGGTTACTTCCCAACAGCCTAAGAAAAGAGTGGCGTTTGTGCTTATCGATGGGTTGGGTGATGTCTCATTACCAAAATTTGGATTTAAGACTCCTTTGCAAGTTGCCAAAGTTCCTAACTTGGATGCGATTGCTTCTGCTGGTGTTAATGGTCTCATGGACCCTGTTGAAGTAGGATTGGGTTGTGGAAGTGACACAGCTCATCTTTCACTTTTGGGCTATGACCCTAGAGTATATTATAGGGGTCGAGGTGCATTTGAGTCCATGGGAGCTGGATTGGCTATGTCACCTGGAGACATTGCGTTTAAGTCAAACTTTGCTACATTGGACGAGAAAACAGGAATAGTAACCAGTAGGAGGGCTGATAGACACTTTGAGGAGGAAGGACCAATATTATGTGCAGCACTTGATAGAATGAAGATACCAAGTTTCCCAGAGTATGAAGTTAGAGTCAGGTATGCCACAGAGCATAGATGTGGCGTGGTTGTGAAAGGACCAAATTTGAGCGGGAACATATCGGGAACAGACCCTTTGAAGGACAACCGCTTACTTTTGCAAGCAGAGGCTTTAGATGATTCTGATGAAGCAAAGCTTACTGCAGCTGTTGTTAATGAGTTATCCAAGGAAATCTCGCGAATTCTGGTTTCTCACCCAATCAATGCAAAGCGTGCAGCAGAAGGGAAGAACATAGCCAACATTGTTCTTTTACGAGGTTGCGGCATTCGAATTGAGGTTCCTCCATTTGAGAAGAAACATGGTTTATGGCCATGCATGGTGGCTCCCACTAAAATTATAGCTGGTCTAGGCTTATCACTAGGAATCGATATCCTAGATGCTCCAGGAGCAACTGGAGATTATCGAACACTCTTAACCTCGAAAGCTACTGCAATGGCAAAGGGACTCTCAGCTCCTTTGCAATCTTGTCCCAGTGTGTTTGTGCCCGGAGAAGATGAGCTCAAACAAGGTCGGGCCGATGGTTACAGTTTCGGTTTCCTTCATGTTAAGGCAATAGATGACGCCGGGCACGATAAGGCAACTATATTCAAAGTTAAGGGAATGGAAGCTGTAGATAGAGCAATAGGACAGCTAGCCAAGCTTCTCTGGAAGGCAGAATCTACTGGAAATTTCCAGTACTACCTCTGTGTGACTGGAGACCACTCAACTCCAGTTGAGTATGGAGATCACAGTTTCGAGCCAGTGCCATTTACAATATGTAGATTGAAAGACTTTGTGGACGCTGTGGGCGGGGAGTCGGTAATTGCAAGCATTTCCCTCGAACCGTTTCCGCTCCCGACGATCGTACCCGGTGAGGACTTGGACTGGAGAGAAATCCAGAAGGTGGAAGAAGAGAGAAGCAAAAACTCCCAAGCTTGTGGTGGTGACTCAGTTTGTGAGTTTGATGAGATTGCAGTTGCCAAGGGATGTCTTGGACGCTTTCCTGGTGGAGAGATGATGGGTATCATAAAGAGATTCCTCCAATTGGATGCTTGA

Protein sequence

MVTSQQPKKRVAFVLIDGLGDVSLPKFGFKTPLQVAKVPNLDAIASAGVNGLMDPVEVGLGCGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRRADRHFEEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKDNRLLLQAEALDDSDEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANIVLLRGCGIRIEVPPFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAMAKGLSAPLQSCPSVFVPGEDELKQGRADGYSFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAIGQLAKLLWKAESTGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTICRLKDFVDAVGGESVIASISLEPFPLPTIVPGEDLDWREIQKVEEERSKNSQACGGDSVCEFDEIAVAKGCLGRFPGGEMMGIIKRFLQLDA

Homology

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BLAST of Lag0006427 vs. NCBI nr
Match: XP_038890619.1 (probable 2,3-bisphosphoglycerate-independent phosphoglycerate mutase [Benincasa hispida] >XP_038890620.1 probable 2,3-bisphosphoglycerate-independent phosphoglycerate mutase [Benincasa hispida])

HSP 1 Score: 957.2 bits (2473), Expect = 5.5e-275
Identity = 469/496 (94.56%), Postives = 483/496 (97.38%), Query Frame = 0


Query: 1   MVTSQQPKKRVAFVLIDGLGDVSLPKFGFKTPLQVAKVPNLDAIASAGVNGLMDPVEVGL 60
           MV SQQPKKRVAFVLIDGLGDVSLPKFGFKTPLQVAKVPNLDAIASAG+NGLMDPVEVGL
Sbjct: 1   MVISQQPKKRVAFVLIDGLGDVSLPKFGFKTPLQVAKVPNLDAIASAGINGLMDPVEVGL 60

Query: 61  GCGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRR 120
           GCGSDTAHLSLLGY+PRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRR
Sbjct: 61  GCGSDTAHLSLLGYNPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRR 120

Query: 121 ADRHFEEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKD 180
           ADRHFEEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKD
Sbjct: 121 ADRHFEEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKD 180

Query: 181 NRLLLQAEALDDSDEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANIVLLRGCG 240
           NRLLLQAEALDDS EAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIAN+VLLRGCG
Sbjct: 181 NRLLLQAEALDDSKEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANLVLLRGCG 240

Query: 241 IRIEVPPFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAMAKG 300
           IRIEVPPFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATA+AKG
Sbjct: 241 IRIEVPPFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAIAKG 300

Query: 301 LSAPLQSCPSVFVPGEDELKQGRADGYSFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAI 360
           LSAPLQSCP+VFVPGEDELK GRADGY FGFLHVKAIDDAGHDKATIFKVKGMEAVDRAI
Sbjct: 301 LSAPLQSCPNVFVPGEDELKPGRADGYDFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAI 360

Query: 361 GQLAKLLWKAESTGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTICRLKDFVDAVGGESVI 420
           GQLAKLLWKAES+GNFQYYLCVTGDHSTPVEYGDHSFEPVPFT+CRLKDFV AVGGESVI
Sbjct: 361 GQLAKLLWKAESSGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTMCRLKDFVGAVGGESVI 420

Query: 421 ASISLEPFPLPTIVPGEDLDWREIQKVEEERSKNSQACGGDSVCEFDEIAVAKGCLGRFP 480
           A ISL+PFPLPT+VPGEDL+ REI+KVEEE++K  QA GGDSVCEFDEI  AKGCLGRFP
Sbjct: 421 AGISLDPFPLPTVVPGEDLERREIEKVEEEKNKECQAFGGDSVCEFDEITAAKGCLGRFP 480

Query: 481 GGEMMGIIKRFLQLDA 497
           GGEMMGIIK+FL LDA
Sbjct: 481 GGEMMGIIKKFLTLDA 496

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BLAST of Lag0006427 vs. NCBI nr
Match: XP_011656023.1 (uncharacterized protein LOC101219404 [Cucumis sativus] >KGN52553.1 hypothetical protein Csa_009366 [Cucumis sativus])

HSP 1 Score: 954.1 bits (2465), Expect = 4.6e-274
Identity = 466/496 (93.95%), Postives = 482/496 (97.18%), Query Frame = 0


Query: 1   MVTSQQPKKRVAFVLIDGLGDVSLPKFGFKTPLQVAKVPNLDAIASAGVNGLMDPVEVGL 60
           M  SQQPKKRVAFVLIDGLGDVS+PKFGFKTPLQVAKVPNLDAIASAG NGLMDPVEVGL
Sbjct: 1   MFISQQPKKRVAFVLIDGLGDVSIPKFGFKTPLQVAKVPNLDAIASAGANGLMDPVEVGL 60

Query: 61  GCGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRR 120
           GCGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRR
Sbjct: 61  GCGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRR 120

Query: 121 ADRHFEEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKD 180
           ADRHFEEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKD
Sbjct: 121 ADRHFEEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKD 180

Query: 181 NRLLLQAEALDDSDEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANIVLLRGCG 240
           NRLLLQAEALDDS+EAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIAN+VLLRGCG
Sbjct: 181 NRLLLQAEALDDSEEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANLVLLRGCG 240

Query: 241 IRIEVPPFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAMAKG 300
           IRIEVPPFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATA+AKG
Sbjct: 241 IRIEVPPFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAIAKG 300

Query: 301 LSAPLQSCPSVFVPGEDELKQGRADGYSFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAI 360
           LSAPLQS P+VFVPGEDELK GRADGY FGFLHVKAIDDAGHDKATIFKVKGMEAVDRAI
Sbjct: 301 LSAPLQSSPNVFVPGEDELKPGRADGYDFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAI 360

Query: 361 GQLAKLLWKAESTGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTICRLKDFVDAVGGESVI 420
           GQLAKLLWKAES+GNFQYYLCVTGDHSTPVEYGDHSFEPVPFT+CRLKDFV AVGGESVI
Sbjct: 361 GQLAKLLWKAESSGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTMCRLKDFVGAVGGESVI 420

Query: 421 ASISLEPFPLPTIVPGEDLDWREIQKVEEERSKNSQACGGDSVCEFDEIAVAKGCLGRFP 480
            +ISL+PFPLPT+VPGEDL+WRE  K+EEER+K  QA GGDSVCEFDEI+ AKGCLGRFP
Sbjct: 421 DAISLDPFPLPTVVPGEDLEWRERHKMEEERNKEGQAFGGDSVCEFDEISAAKGCLGRFP 480

Query: 481 GGEMMGIIKRFLQLDA 497
           GGEMMGIIK+FL+LDA
Sbjct: 481 GGEMMGIIKKFLKLDA 496

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BLAST of Lag0006427 vs. NCBI nr
Match: KAA0039399.1 (putative 2,3-bisphosphoglycerate-independent phosphoglycerate mutase [Cucumis melo var. makuwa] >TYK00587.1 putative 2,3-bisphosphoglycerate-independent phosphoglycerate mutase [Cucumis melo var. makuwa])

HSP 1 Score: 951.8 bits (2459), Expect = 2.3e-273
Identity = 464/496 (93.55%), Postives = 481/496 (96.98%), Query Frame = 0


Query: 1   MVTSQQPKKRVAFVLIDGLGDVSLPKFGFKTPLQVAKVPNLDAIASAGVNGLMDPVEVGL 60
           MV SQQPKKRVAFVLIDGLGDVS+PKFGFKTPLQVAKVPNLDAIASAGVNGLMDPVEVGL
Sbjct: 1   MVISQQPKKRVAFVLIDGLGDVSIPKFGFKTPLQVAKVPNLDAIASAGVNGLMDPVEVGL 60

Query: 61  GCGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRR 120
           GCGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIV+SRR
Sbjct: 61  GCGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVSSRR 120

Query: 121 ADRHFEEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKD 180
           ADRHFEEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKD
Sbjct: 121 ADRHFEEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKD 180

Query: 181 NRLLLQAEALDDSDEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANIVLLRGCG 240
           NRLLLQAEALDDS+EAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIAN+VLLRGCG
Sbjct: 181 NRLLLQAEALDDSEEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANLVLLRGCG 240

Query: 241 IRIEVPPFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAMAKG 300
           IRIEVPPFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATA+AKG
Sbjct: 241 IRIEVPPFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAIAKG 300

Query: 301 LSAPLQSCPSVFVPGEDELKQGRADGYSFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAI 360
           LSAPL SCP+VFVPGEDELK GRADGY FGFLHVKAIDDAGHDKATIFKVKGMEAVDRAI
Sbjct: 301 LSAPLHSCPNVFVPGEDELKPGRADGYDFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAI 360

Query: 361 GQLAKLLWKAESTGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTICRLKDFVDAVGGESVI 420
           GQLAKLLWKAE +GNFQYYLCVTGDHSTPVEYGDHSFEPVPFT+CRLKD V AVGGESVI
Sbjct: 361 GQLAKLLWKAECSGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTMCRLKDLVGAVGGESVI 420

Query: 421 ASISLEPFPLPTIVPGEDLDWREIQKVEEERSKNSQACGGDSVCEFDEIAVAKGCLGRFP 480
             ISL+PFPLPT+VPGEDL+WRE  K+EEER++  QA GGDSVCEFDEI+ AKGCLGRFP
Sbjct: 421 DGISLDPFPLPTVVPGEDLEWRESHKMEEERNQECQAFGGDSVCEFDEISAAKGCLGRFP 480

Query: 481 GGEMMGIIKRFLQLDA 497
           GGEMMGIIK+FL+LDA
Sbjct: 481 GGEMMGIIKKFLKLDA 496

[Close]

BLAST of Lag0006427 vs. NCBI nr
Match: XP_022990600.1 (uncharacterized protein LOC111487437 [Cucurbita maxima] >XP_022990601.1 uncharacterized protein LOC111487437 [Cucurbita maxima] >XP_022990602.1 uncharacterized protein LOC111487437 [Cucurbita maxima] >XP_022990603.1 uncharacterized protein LOC111487437 [Cucurbita maxima] >XP_022990604.1 uncharacterized protein LOC111487437 [Cucurbita maxima] >XP_022990605.1 uncharacterized protein LOC111487437 [Cucurbita maxima] >XP_022990606.1 uncharacterized protein LOC111487437 [Cucurbita maxima] >XP_022990608.1 uncharacterized protein LOC111487437 [Cucurbita maxima] >XP_022990609.1 uncharacterized protein LOC111487437 [Cucurbita maxima] >XP_022990610.1 uncharacterized protein LOC111487437 [Cucurbita maxima])

HSP 1 Score: 942.6 bits (2435), Expect = 1.4e-270
Identity = 465/496 (93.75%), Postives = 478/496 (96.37%), Query Frame = 0


Query: 1   MVTSQQPKKRVAFVLIDGLGDVSLPKFGFKTPLQVAKVPNLDAIASAGVNGLMDPVEVGL 60
           MVTSQQPK+RVAFVLIDGLGDVS+PKFGFKTPLQVAKVPNLDAIASAGVNGLMDPVEVGL
Sbjct: 1   MVTSQQPKRRVAFVLIDGLGDVSIPKFGFKTPLQVAKVPNLDAIASAGVNGLMDPVEVGL 60

Query: 61  GCGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRR 120
           GCGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEK+GIVTSRR
Sbjct: 61  GCGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKSGIVTSRR 120

Query: 121 ADRHFEEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKD 180
           ADRHFEEEGPILCA+LDR+KIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKD
Sbjct: 121 ADRHFEEEGPILCASLDRLKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKD 180

Query: 181 NRLLLQAEALDDSDEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANIVLLRGCG 240
           NRLLLQAEALDDS EAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIAN+VLLRGCG
Sbjct: 181 NRLLLQAEALDDSKEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANLVLLRGCG 240

Query: 241 IRIEVPPFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAMAKG 300
           IRIEVPPFEKKH LWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATA+AKG
Sbjct: 241 IRIEVPPFEKKHELWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAIAKG 300

Query: 301 LSAPLQSCPSVFVPGEDELKQGRADGYSFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAI 360
           LSAPLQSCP+VFVPGE+ELK GR DGY FGFLHVKAIDDAGHDKATIFKVKGMEAVDRAI
Sbjct: 301 LSAPLQSCPNVFVPGENELKPGRTDGYDFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAI 360

Query: 361 GQLAKLLWKAESTGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTICRLKDFVDAVGGESVI 420
           GQLAKLLWKAESTG FQYYLCVTGDHSTPVEYGDHSFEPVPFTICRLKDFV AVGGESVI
Sbjct: 361 GQLAKLLWKAESTGKFQYYLCVTGDHSTPVEYGDHSFEPVPFTICRLKDFVGAVGGESVI 420

Query: 421 ASISLEPFPLPTIVPGEDLDWREIQKVEEERSKNSQACGGDSVCEFDEIAVAKGCLGRFP 480
           A ISL+PFPLPTIV  EDLDW     V EERSK+SQA GGDSVCEFDEI+ AKGCLGRFP
Sbjct: 421 AGISLDPFPLPTIVASEDLDW-----VSEERSKDSQAFGGDSVCEFDEISAAKGCLGRFP 480

Query: 481 GGEMMGIIKRFLQLDA 497
           GGEMMGIIKRFL+LDA
Sbjct: 481 GGEMMGIIKRFLKLDA 491

[Close]

BLAST of Lag0006427 vs. NCBI nr
Match: XP_022134154.1 (uncharacterized protein LOC111006490 [Momordica charantia] >XP_022134155.1 uncharacterized protein LOC111006490 [Momordica charantia])

HSP 1 Score: 936.0 bits (2418), Expect = 1.3e-268
Identity = 463/496 (93.35%), Postives = 475/496 (95.77%), Query Frame = 0


Query: 1   MVTSQQPKKRVAFVLIDGLGDVSLPKFGFKTPLQVAKVPNLDAIASAGVNGLMDPVEVGL 60
           MV  QQPKKRVAFVLIDGLGDVSLPKFGF TPLQVAKVPNLDAIASAGVNGLMDPVEVGL
Sbjct: 1   MVNPQQPKKRVAFVLIDGLGDVSLPKFGFMTPLQVAKVPNLDAIASAGVNGLMDPVEVGL 60

Query: 61  GCGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRR 120
           GCGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAM PGDIAFKSNFATLDEKTGIVTSRR
Sbjct: 61  GCGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAMLPGDIAFKSNFATLDEKTGIVTSRR 120

Query: 121 ADRHFEEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKD 180
           ADRHFEEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKD
Sbjct: 121 ADRHFEEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKD 180

Query: 181 NRLLLQAEALDDSDEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANIVLLRGCG 240
           NRLLLQAEALD SDEAKLTAAVVNELSKEISRILVSHPINAKRAAE KNIANIVLLRGCG
Sbjct: 181 NRLLLQAEALDGSDEAKLTAAVVNELSKEISRILVSHPINAKRAAERKNIANIVLLRGCG 240

Query: 241 IRIEVPPFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAMAKG 300
           IRIEVP FEKKH LWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATA+AKG
Sbjct: 241 IRIEVPSFEKKHDLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAIAKG 300

Query: 301 LSAPLQSCPSVFVPGEDELKQGRADGYSFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAI 360
           LSAPLQ+CP+VFVPGEDE K GRADGY FGFLHVKAIDDAGHDKATIFKVKGMEAVD+AI
Sbjct: 301 LSAPLQACPNVFVPGEDEFKPGRADGYDFGFLHVKAIDDAGHDKATIFKVKGMEAVDKAI 360

Query: 361 GQLAKLLWKAESTGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTICRLKDFVDAVGGESVI 420
           GQLAKLLWK+ESTG+FQYYLCVTGDHSTPVEYGDHSFEPVPF ICRLKDFV AVGGESVI
Sbjct: 361 GQLAKLLWKSESTGDFQYYLCVTGDHSTPVEYGDHSFEPVPFAICRLKDFVGAVGGESVI 420

Query: 421 ASISLEPFPLPTIVPGEDLDWREIQKVEEERSKNSQACGGDSVCEFDEIAVAKGCLGRFP 480
           A ISL+PFPLPTIVPGEDLDWRE+QKV E RSK  +A GGDSV EFDEIA A+GCLGRFP
Sbjct: 421 AGISLDPFPLPTIVPGEDLDWREVQKV-EGRSKECEAFGGDSVWEFDEIAAARGCLGRFP 480

Query: 481 GGEMMGIIKRFLQLDA 497
           GGEMMGIIK+FL+LDA
Sbjct: 481 GGEMMGIIKKFLKLDA 495

[Close]

BLAST of Lag0006427 vs. ExPASy Swiss-Prot
Match: O27628 (2,3-bisphosphoglycerate-independent phosphoglycerate mutase 1 OS=Methanothermobacter thermautotrophicus (strain ATCC 29096 / DSM 1053 / JCM 10044 / NBRC 100330 / Delta H) OX=187420 GN=apgM1 PE=3 SV=1)

HSP 1 Score: 265.0 bits (676), Expect = 1.7e-69
Identity = 152/401 (37.91%), Postives = 216/401 (53.87%), Query Frame = 0


Query: 14  VLIDGLGDVSLPKFGFKTPLQVAKVPNLDAIASAGVNGLMDPVEVGLGCGSDTAHLSLLG 73
           ++IDG+ D  L K   +TPL+ A  PN+D IA +G+NG+MDP++ G+  GSDT+HLS+LG
Sbjct: 6   MIIDGMADRPLEKLSGRTPLEAASTPNMDRIAESGINGIMDPIKPGVRAGSDTSHLSILG 65

Query: 74  YDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRRADRHFEEEGPILC 133
           YDP   Y GRG FE+ G GL + PGDIAF+ NFAT DE   I+T RRA R        + 
Sbjct: 66  YDPYRVYTGRGPFEAAGVGLDVKPGDIAFRCNFATADEDM-IITDRRAGR-IRSGTSSIA 125

Query: 134 AALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKDNRLLLQAEALDDS 193
             ++ M I  F + E+  R +T HR  +V++GP L   +S  DP  + +   + +ALD S
Sbjct: 126 RTINSMTIEGFEDVEIIFRESTGHRAVLVLRGPGLGDKVSDADPKVEGKPPKKIKALDGS 185

Query: 194 DEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANIVLLRGCGIRIEVPPFEKKHG 253
            E++ TA V+N + KE   IL  HP+N  R   G+  ANI+L RG G    V  F++++ 
Sbjct: 186 PESRKTAEVLNRIVKESYEILRDHPVNTGRIERGEAPANIILPRGAGAVPHVEKFQERYD 245

Query: 254 LWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAMAKGLSAPLQSCPSVFV 313
           L    +A T +I G+G   G+D++D  GATG   T L S   A++  ++A          
Sbjct: 246 LKAACIAETGLIKGIGHLTGMDVIDVEGATGGIDTDLESIKNAISSAIAA---------- 305

Query: 314 PGEDELKQGRADGYSFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAIGQLAKLLWKAEST 373
                        Y F  +++   D+AGHD     KV+ +E VD  +G L          
Sbjct: 306 ------------DYDFLLINIDGADEAGHDGDLEGKVRFIERVDSILGDLM--------- 365

Query: 374 GNFQYYLCVTGDHSTPVEYGDHSFEPVPFTICRLKDFVDAV 415
                Y  +T DHSTPV   DH+ +PVP  I   +  VD V
Sbjct: 366 -GDDIYFILTADHSTPVSVMDHTGDPVPIAITGPEVRVDDV 372

[Close]

BLAST of Lag0006427 vs. ExPASy Swiss-Prot
Match: A4YIX4 (2,3-bisphosphoglycerate-independent phosphoglycerate mutase OS=Metallosphaera sedula (strain ATCC 51363 / DSM 5348 / JCM 9185 / NBRC 15509 / TH2) OX=399549 GN=apgM PE=3 SV=1)

HSP 1 Score: 262.7 bits (670), Expect = 8.4e-69
Identity = 152/397 (38.29%), Postives = 223/397 (56.17%), Query Frame = 0


Query: 8   KKRVAFVLIDGLGDVSLPKFGFKTPLQVAKVPNLDAIASAGVNGLMDPVEVGLGCGSDTA 67
           K ++  V+ DGLGD  +     +TPL+ A  P + ++  + + GLMDP+  G+  GSDT+
Sbjct: 3   KYKILLVIGDGLGDRQVASLNGRTPLENADKPTIASLLRSSLVGLMDPIGPGIVPGSDTS 62

Query: 68  HLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRRADRHFEE 127
           HL++ G DP+ YY+GRG+FE++GAG  ++ GDIAF+ NFAT+D    +V  RRA R  EE
Sbjct: 63  HLAIFGLDPKKYYKGRGSFEALGAGAILTEGDIAFRGNFATVDSNL-VVIDRRAGRKIEE 122

Query: 128 EGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKDNRLLLQA 187
              ++    D  KI      +VR  + TEHR  VV+ G NLS  +S TDP +  + +L +
Sbjct: 123 AEDLVKELND--KIQEIDGVKVRFYHGTEHRVSVVLSGDNLSDKVSDTDPHEVGKRILNS 182

Query: 188 EALDDSDEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANIVLLRGCGIRIEVPP 247
           E  DD+  +K TA ++N L++ I  +L +  +N KR  EG   ANIVLLRG  I  E+P 
Sbjct: 183 EPTDDALSSKRTANIINALTRRIYEVLSNSQLNDKRVREGLPPANIVLLRGASIHTELPK 242

Query: 248 FEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAMAKGLSAPLQS 307
            +   GL    V+ T +I G+  SLG++++  PGATG   T   +KA A AK L      
Sbjct: 243 LKDYTGLSGAAVSATALIKGVCKSLGMEVVTPPGATGGIDTDYMAKAEAAAKLL------ 302

Query: 308 CPSVFVPGEDELKQGRADGYSFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAIGQLAKLL 367
                            + +   FLH+KA D A HD     KVK +E +DR+IG++    
Sbjct: 303 -----------------EDHDLVFLHIKATDAASHDGKVSEKVKAIEMIDRSIGRV---- 362

Query: 368 WKAESTGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTI 405
              +  G+ +  +  TGDH+TPVE  +HS +PVP  +
Sbjct: 363 --LDRYGS-ELVVLFTGDHATPVELREHSGDPVPLML 366

[Close]

BLAST of Lag0006427 vs. ExPASy Swiss-Prot
Match: P58813 (2,3-bisphosphoglycerate-independent phosphoglycerate mutase OS=Methanopyrus kandleri (strain AV19 / DSM 6324 / JCM 9639 / NBRC 100938) OX=190192 GN=apgM PE=3 SV=1)

HSP 1 Score: 256.1 bits (653), Expect = 7.9e-67
Identity = 158/485 (32.58%), Postives = 242/485 (49.90%), Query Frame = 0


Query: 8   KKRVAFVLIDGLGDVSLPKFGFKTPLQVAKVPNLDAIASAGVNGLMDPVEVGLGCGSDTA 67
           ++++  ++ DG+ D ++P+   KTPLQ A  PN+D +A  G  GL+DP+  G+  GSDTA
Sbjct: 7   ERKILVIVGDGMADRAVPELDGKTPLQAADTPNMDRLAREGSVGLLDPIRPGVRPGSDTA 66

Query: 68  HLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTG--IVTSRRADRHF 127
           HL+LLGYDP   Y GRG  E++GAG+ + PGD+AF+ NFAT +E+ G  +V  RRA R  
Sbjct: 67  HLTLLGYDPFEVYPGRGPLEALGAGVEVRPGDVAFRCNFATAEERNGELVVVDRRAGRIN 126

Query: 128 EEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKDNRLLL 187
           E+EG    A     ++    E+E   + A  HR  +V++G +LS +++  DP +  + + 
Sbjct: 127 EDEGTPKLAETINEEVDLPVEFE--FKEAVGHRAVLVLRGGDLSADVTDADPKRVGKPVK 186

Query: 188 QAEALDDSDEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANIVLLRGCGIRIEV 247
             +   D   A  TA +VNE  ++   +L  HP+N +R  +GK  AN++L RG G   EV
Sbjct: 187 DVKPTSDDPAAARTAEIVNEFVRKAYEVLKDHPVNRERERQGKPPANVILPRGAGQLEEV 246

Query: 248 PPFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAMAKGLSAPL 307
            PF  ++G+   +VA   +I G+G  LG+D+ +    TG   T L  KA           
Sbjct: 247 EPFSDRYGMSGAVVAGASLIKGIGRMLGMDVPEDEAITGRKDTDLKRKA----------- 306

Query: 308 QSCPSVFVPGEDELKQGRADGYSFGFLHVKAIDDAGHDKATIFKVKGMEAVDR-AIGQLA 367
                       EL     D +    ++  A+D+AGHD     KV+ +E +DR  +G L 
Sbjct: 307 ------------ELALEALDDHDLVLVNFNAVDEAGHDGDARGKVEMIERMDRELVGTLL 366

Query: 368 KLLWKAESTGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTICRLKDFVDAVGGESVIASIS 427
           + +   E+       +C+T DHSTPV  GDH+ +PVP  I                    
Sbjct: 367 EGIDPEETV------VCLTADHSTPVAVGDHTADPVPVAI-------------------- 411

Query: 428 LEPFPLPTIVPGEDLDWREIQKVEEERSKNSQACGGDSVCEFDEIAVAKGCLGRFPGGEM 487
                           W             +     D V E+DEI+ A+GCLGRF G  +
Sbjct: 427 ----------------W-------------TADARRDPVEEYDEISAARGCLGRFSGLHL 411

Query: 488 MGIIK 490
           + +++
Sbjct: 487 LNVLR 411

[Close]

BLAST of Lag0006427 vs. ExPASy Swiss-Prot
Match: Q980A0 (2,3-bisphosphoglycerate-independent phosphoglycerate mutase OS=Saccharolobus solfataricus (strain ATCC 35092 / DSM 1617 / JCM 11322 / P2) OX=273057 GN=apgM PE=3 SV=1)

HSP 1 Score: 255.8 bits (652), Expect = 1.0e-66
Identity = 149/392 (38.01%), Postives = 217/392 (55.36%), Query Frame = 0


Query: 10  RVAFVLIDGLGDVSLPKFGFKTPLQVAKVPNLDAIASAGVNGLMDPVEVGLGCGSDTAHL 69
           ++  ++ DGLGD  + K    TPL+ A  P +  +    + GLMDP+  G+  GSDT+HL
Sbjct: 5   KILLIVADGLGDRPVSKLNGLTPLEAANKPAITDLLKNSMIGLMDPISPGIIPGSDTSHL 64

Query: 70  SLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRRADRHFEEEG 129
           S+ G DP VYYRGRGAFE++GAG  +S GD+AF+ NFAT++    +V  RRA R   EEG
Sbjct: 65  SIFGLDPHVYYRGRGAFEALGAGATLSHGDVAFRGNFATVNNDL-VVVDRRAGRKL-EEG 124

Query: 130 PILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKDNRLLLQAEA 189
             L   L+  KI    + +++    TEHR  VV+ G  +S  +S TDP  +   +L+++ 
Sbjct: 125 EQLVKELNE-KIKEINDVKIKFYKGTEHRIAVVLSGKGISDKVSDTDPHHEGLKVLESKP 184

Query: 190 LDDSDEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANIVLLRGCGIRIEVPPFE 249
           L+DS+EA  TA ++N L++++  +L S  IN +R  +G+  ANIVLLRG    +++PPF 
Sbjct: 185 LEDSNEAIRTAEIINILTRKVFDVLNSSEINKRRIEQGEKPANIVLLRGAAHYVKLPPFS 244

Query: 250 KKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAMAKGLSAPLQSCP 309
               L    V+ T +I G+   LG++++   GATG   T   +KA A             
Sbjct: 245 SYTKLKAAAVSATALIKGICRELGMNVITPSGATGGIDTNYNAKAKAAI----------- 304

Query: 310 SVFVPGEDELKQGRADGYSFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAIGQLAKLLWK 369
                  + LK+       F FLH+KA D A HD     KVK +E +DR IG +      
Sbjct: 305 -------ELLKEN-----DFVFLHIKATDAASHDGLVEEKVKAIERIDRVIGAI------ 364

Query: 370 AESTGNFQYYLCVTGDHSTPVEYGDHSFEPVP 402
            ++ G     L  TGDH TPVE  +H+ +PVP
Sbjct: 365 IDNIGRDNLILMFTGDHVTPVEIKEHTGDPVP 364

[Close]

BLAST of Lag0006427 vs. ExPASy Swiss-Prot
Match: Q975P3 (2,3-bisphosphoglycerate-independent phosphoglycerate mutase OS=Sulfurisphaera tokodaii (strain DSM 16993 / JCM 10545 / NBRC 100140 / 7) OX=273063 GN=apgM PE=3 SV=1)

HSP 1 Score: 255.8 bits (652), Expect = 1.0e-66
Identity = 148/405 (36.54%), Postives = 221/405 (54.57%), Query Frame = 0


Query: 10  RVAFVLIDGLGDVSLPKFGFKTPLQVAKVPNLDAIASAGVNGLMDPVEVGLGCGSDTAHL 69
           ++ F + DGLGD  + K   KTPL+    PN+  +    + GLMDP+  G+  GSDT+HL
Sbjct: 5   KILFFIADGLGDRPVRKLQGKTPLEYVDKPNIRELLKNSIIGLMDPISPGVVAGSDTSHL 64

Query: 70  SLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRRADRHFEEEG 129
           S+ G DP  YYRGRGAFE++GAG  +   D+AF+ NFAT++ +  IV  RRA R  EE  
Sbjct: 65  SMFGLDPHKYYRGRGAFEAIGAGARLKASDVAFRGNFATVNNEF-IVVDRRAGRKIEEAD 124

Query: 130 PILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKDNRLLLQAEA 189
            ++    +  KI      +VR  + TEHR  VV+ G  L+  +S TDP + N+ +L+++ 
Sbjct: 125 DLVKELNE--KIGEIDGVKVRFYHGTEHRVAVVLSGKGLTDKVSDTDPHEVNKKVLESKP 184

Query: 190 LDDSDEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANIVLLRGCGIRIEVPPFE 249
           LD+S E++ TA ++N+L+++I  IL S  IN  R ++G+  ANI+LLRG    +E+P FE
Sbjct: 185 LDNSPESQFTANIINKLTRKIYEILNSSEINKIRVSKGELPANIILLRGAAEFVELPQFE 244

Query: 250 KKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAMAKGLSAPLQSCP 309
               L    V+ T +I G+   +G+ ++  PGATG   T    KA A  + L        
Sbjct: 245 SYTKLKAAAVSATALIKGICEQIGMRVVTPPGATGGLDTNYLGKADAAVELLKE------ 304

Query: 310 SVFVPGEDELKQGRADGYSFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAIGQLAKLLWK 369
                            Y F FLH+KA D A HD     KV  +  +D  IG++   L  
Sbjct: 305 -----------------YDFVFLHLKATDAASHDGNVDGKVYAINKMDEMIGRILDKL-- 364

Query: 370 AESTGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTICRLKDFVDAV 415
                  +  + ++GDH+TPVE  +H+ +PVPF +    D ++ V
Sbjct: 365 -----GSELVIAISGDHTTPVEVKEHTGDPVPFLLYVPYDIINDV 376

[Close]

BLAST of Lag0006427 vs. ExPASy TrEMBL
Match: A0A0A0KSM0 (Metalloenzyme domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G643310 PE=3 SV=1)

HSP 1 Score: 954.1 bits (2465), Expect = 2.2e-274
Identity = 466/496 (93.95%), Postives = 482/496 (97.18%), Query Frame = 0


Query: 1   MVTSQQPKKRVAFVLIDGLGDVSLPKFGFKTPLQVAKVPNLDAIASAGVNGLMDPVEVGL 60
           M  SQQPKKRVAFVLIDGLGDVS+PKFGFKTPLQVAKVPNLDAIASAG NGLMDPVEVGL
Sbjct: 1   MFISQQPKKRVAFVLIDGLGDVSIPKFGFKTPLQVAKVPNLDAIASAGANGLMDPVEVGL 60

Query: 61  GCGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRR 120
           GCGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRR
Sbjct: 61  GCGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRR 120

Query: 121 ADRHFEEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKD 180
           ADRHFEEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKD
Sbjct: 121 ADRHFEEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKD 180

Query: 181 NRLLLQAEALDDSDEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANIVLLRGCG 240
           NRLLLQAEALDDS+EAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIAN+VLLRGCG
Sbjct: 181 NRLLLQAEALDDSEEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANLVLLRGCG 240

Query: 241 IRIEVPPFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAMAKG 300
           IRIEVPPFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATA+AKG
Sbjct: 241 IRIEVPPFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAIAKG 300

Query: 301 LSAPLQSCPSVFVPGEDELKQGRADGYSFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAI 360
           LSAPLQS P+VFVPGEDELK GRADGY FGFLHVKAIDDAGHDKATIFKVKGMEAVDRAI
Sbjct: 301 LSAPLQSSPNVFVPGEDELKPGRADGYDFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAI 360

Query: 361 GQLAKLLWKAESTGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTICRLKDFVDAVGGESVI 420
           GQLAKLLWKAES+GNFQYYLCVTGDHSTPVEYGDHSFEPVPFT+CRLKDFV AVGGESVI
Sbjct: 361 GQLAKLLWKAESSGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTMCRLKDFVGAVGGESVI 420

Query: 421 ASISLEPFPLPTIVPGEDLDWREIQKVEEERSKNSQACGGDSVCEFDEIAVAKGCLGRFP 480
            +ISL+PFPLPT+VPGEDL+WRE  K+EEER+K  QA GGDSVCEFDEI+ AKGCLGRFP
Sbjct: 421 DAISLDPFPLPTVVPGEDLEWRERHKMEEERNKEGQAFGGDSVCEFDEISAAKGCLGRFP 480

Query: 481 GGEMMGIIKRFLQLDA 497
           GGEMMGIIK+FL+LDA
Sbjct: 481 GGEMMGIIKKFLKLDA 496

[Close]

BLAST of Lag0006427 vs. ExPASy TrEMBL
Match: A0A5D3BN00 (Putative 2,3-bisphosphoglycerate-independent phosphoglycerate mutase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold169G002040 PE=3 SV=1)

HSP 1 Score: 951.8 bits (2459), Expect = 1.1e-273
Identity = 464/496 (93.55%), Postives = 481/496 (96.98%), Query Frame = 0


Query: 1   MVTSQQPKKRVAFVLIDGLGDVSLPKFGFKTPLQVAKVPNLDAIASAGVNGLMDPVEVGL 60
           MV SQQPKKRVAFVLIDGLGDVS+PKFGFKTPLQVAKVPNLDAIASAGVNGLMDPVEVGL
Sbjct: 1   MVISQQPKKRVAFVLIDGLGDVSIPKFGFKTPLQVAKVPNLDAIASAGVNGLMDPVEVGL 60

Query: 61  GCGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRR 120
           GCGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIV+SRR
Sbjct: 61  GCGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVSSRR 120

Query: 121 ADRHFEEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKD 180
           ADRHFEEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKD
Sbjct: 121 ADRHFEEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKD 180

Query: 181 NRLLLQAEALDDSDEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANIVLLRGCG 240
           NRLLLQAEALDDS+EAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIAN+VLLRGCG
Sbjct: 181 NRLLLQAEALDDSEEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANLVLLRGCG 240

Query: 241 IRIEVPPFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAMAKG 300
           IRIEVPPFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATA+AKG
Sbjct: 241 IRIEVPPFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAIAKG 300

Query: 301 LSAPLQSCPSVFVPGEDELKQGRADGYSFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAI 360
           LSAPL SCP+VFVPGEDELK GRADGY FGFLHVKAIDDAGHDKATIFKVKGMEAVDRAI
Sbjct: 301 LSAPLHSCPNVFVPGEDELKPGRADGYDFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAI 360

Query: 361 GQLAKLLWKAESTGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTICRLKDFVDAVGGESVI 420
           GQLAKLLWKAE +GNFQYYLCVTGDHSTPVEYGDHSFEPVPFT+CRLKD V AVGGESVI
Sbjct: 361 GQLAKLLWKAECSGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTMCRLKDLVGAVGGESVI 420

Query: 421 ASISLEPFPLPTIVPGEDLDWREIQKVEEERSKNSQACGGDSVCEFDEIAVAKGCLGRFP 480
             ISL+PFPLPT+VPGEDL+WRE  K+EEER++  QA GGDSVCEFDEI+ AKGCLGRFP
Sbjct: 421 DGISLDPFPLPTVVPGEDLEWRESHKMEEERNQECQAFGGDSVCEFDEISAAKGCLGRFP 480

Query: 481 GGEMMGIIKRFLQLDA 497
           GGEMMGIIK+FL+LDA
Sbjct: 481 GGEMMGIIKKFLKLDA 496

[Close]

BLAST of Lag0006427 vs. ExPASy TrEMBL
Match: A0A6J1JTS4 (uncharacterized protein LOC111487437 OS=Cucurbita maxima OX=3661 GN=LOC111487437 PE=3 SV=1)

HSP 1 Score: 942.6 bits (2435), Expect = 6.8e-271
Identity = 465/496 (93.75%), Postives = 478/496 (96.37%), Query Frame = 0


Query: 1   MVTSQQPKKRVAFVLIDGLGDVSLPKFGFKTPLQVAKVPNLDAIASAGVNGLMDPVEVGL 60
           MVTSQQPK+RVAFVLIDGLGDVS+PKFGFKTPLQVAKVPNLDAIASAGVNGLMDPVEVGL
Sbjct: 1   MVTSQQPKRRVAFVLIDGLGDVSIPKFGFKTPLQVAKVPNLDAIASAGVNGLMDPVEVGL 60

Query: 61  GCGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRR 120
           GCGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEK+GIVTSRR
Sbjct: 61  GCGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKSGIVTSRR 120

Query: 121 ADRHFEEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKD 180
           ADRHFEEEGPILCA+LDR+KIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKD
Sbjct: 121 ADRHFEEEGPILCASLDRLKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKD 180

Query: 181 NRLLLQAEALDDSDEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANIVLLRGCG 240
           NRLLLQAEALDDS EAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIAN+VLLRGCG
Sbjct: 181 NRLLLQAEALDDSKEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANLVLLRGCG 240

Query: 241 IRIEVPPFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAMAKG 300
           IRIEVPPFEKKH LWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATA+AKG
Sbjct: 241 IRIEVPPFEKKHELWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAIAKG 300

Query: 301 LSAPLQSCPSVFVPGEDELKQGRADGYSFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAI 360
           LSAPLQSCP+VFVPGE+ELK GR DGY FGFLHVKAIDDAGHDKATIFKVKGMEAVDRAI
Sbjct: 301 LSAPLQSCPNVFVPGENELKPGRTDGYDFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAI 360

Query: 361 GQLAKLLWKAESTGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTICRLKDFVDAVGGESVI 420
           GQLAKLLWKAESTG FQYYLCVTGDHSTPVEYGDHSFEPVPFTICRLKDFV AVGGESVI
Sbjct: 361 GQLAKLLWKAESTGKFQYYLCVTGDHSTPVEYGDHSFEPVPFTICRLKDFVGAVGGESVI 420

Query: 421 ASISLEPFPLPTIVPGEDLDWREIQKVEEERSKNSQACGGDSVCEFDEIAVAKGCLGRFP 480
           A ISL+PFPLPTIV  EDLDW     V EERSK+SQA GGDSVCEFDEI+ AKGCLGRFP
Sbjct: 421 AGISLDPFPLPTIVASEDLDW-----VSEERSKDSQAFGGDSVCEFDEISAAKGCLGRFP 480

Query: 481 GGEMMGIIKRFLQLDA 497
           GGEMMGIIKRFL+LDA
Sbjct: 481 GGEMMGIIKRFLKLDA 491

[Close]

BLAST of Lag0006427 vs. ExPASy TrEMBL
Match: A0A6J1BXB2 (uncharacterized protein LOC111006490 OS=Momordica charantia OX=3673 GN=LOC111006490 PE=3 SV=1)

HSP 1 Score: 936.0 bits (2418), Expect = 6.3e-269
Identity = 463/496 (93.35%), Postives = 475/496 (95.77%), Query Frame = 0


Query: 1   MVTSQQPKKRVAFVLIDGLGDVSLPKFGFKTPLQVAKVPNLDAIASAGVNGLMDPVEVGL 60
           MV  QQPKKRVAFVLIDGLGDVSLPKFGF TPLQVAKVPNLDAIASAGVNGLMDPVEVGL
Sbjct: 1   MVNPQQPKKRVAFVLIDGLGDVSLPKFGFMTPLQVAKVPNLDAIASAGVNGLMDPVEVGL 60

Query: 61  GCGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRR 120
           GCGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAM PGDIAFKSNFATLDEKTGIVTSRR
Sbjct: 61  GCGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAMLPGDIAFKSNFATLDEKTGIVTSRR 120

Query: 121 ADRHFEEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKD 180
           ADRHFEEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKD
Sbjct: 121 ADRHFEEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKD 180

Query: 181 NRLLLQAEALDDSDEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANIVLLRGCG 240
           NRLLLQAEALD SDEAKLTAAVVNELSKEISRILVSHPINAKRAAE KNIANIVLLRGCG
Sbjct: 181 NRLLLQAEALDGSDEAKLTAAVVNELSKEISRILVSHPINAKRAAERKNIANIVLLRGCG 240

Query: 241 IRIEVPPFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAMAKG 300
           IRIEVP FEKKH LWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATA+AKG
Sbjct: 241 IRIEVPSFEKKHDLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAIAKG 300

Query: 301 LSAPLQSCPSVFVPGEDELKQGRADGYSFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAI 360
           LSAPLQ+CP+VFVPGEDE K GRADGY FGFLHVKAIDDAGHDKATIFKVKGMEAVD+AI
Sbjct: 301 LSAPLQACPNVFVPGEDEFKPGRADGYDFGFLHVKAIDDAGHDKATIFKVKGMEAVDKAI 360

Query: 361 GQLAKLLWKAESTGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTICRLKDFVDAVGGESVI 420
           GQLAKLLWK+ESTG+FQYYLCVTGDHSTPVEYGDHSFEPVPF ICRLKDFV AVGGESVI
Sbjct: 361 GQLAKLLWKSESTGDFQYYLCVTGDHSTPVEYGDHSFEPVPFAICRLKDFVGAVGGESVI 420

Query: 421 ASISLEPFPLPTIVPGEDLDWREIQKVEEERSKNSQACGGDSVCEFDEIAVAKGCLGRFP 480
           A ISL+PFPLPTIVPGEDLDWRE+QKV E RSK  +A GGDSV EFDEIA A+GCLGRFP
Sbjct: 421 AGISLDPFPLPTIVPGEDLDWREVQKV-EGRSKECEAFGGDSVWEFDEIAAARGCLGRFP 480

Query: 481 GGEMMGIIKRFLQLDA 497
           GGEMMGIIK+FL+LDA
Sbjct: 481 GGEMMGIIKKFLKLDA 495

[Close]

BLAST of Lag0006427 vs. ExPASy TrEMBL
Match: A0A6J1HGY7 (uncharacterized protein LOC111463418 OS=Cucurbita moschata OX=3662 GN=LOC111463418 PE=3 SV=1)

HSP 1 Score: 932.9 bits (2410), Expect = 5.4e-268
Identity = 458/496 (92.34%), Postives = 475/496 (95.77%), Query Frame = 0


Query: 1   MVTSQQPKKRVAFVLIDGLGDVSLPKFGFKTPLQVAKVPNLDAIASAGVNGLMDPVEVGL 60
           MVTSQQPK+RVAFVLIDGLGDVS+PKFGFKTPLQVAKVPNLDAIASAGVNGLMDPVEVGL
Sbjct: 1   MVTSQQPKRRVAFVLIDGLGDVSIPKFGFKTPLQVAKVPNLDAIASAGVNGLMDPVEVGL 60

Query: 61  GCGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRR 120
           GCGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEK+GIVTSRR
Sbjct: 61  GCGSDTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKSGIVTSRR 120

Query: 121 ADRHFEEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKD 180
           ADRHFEEEGPILCA+LDR+KIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKD
Sbjct: 121 ADRHFEEEGPILCASLDRLKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKD 180

Query: 181 NRLLLQAEALDDSDEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANIVLLRGCG 240
           NRLLLQAEALDDS EAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIAN+VLLRGCG
Sbjct: 181 NRLLLQAEALDDSKEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANLVLLRGCG 240

Query: 241 IRIEVPPFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAMAKG 300
           IRIEVPPFEKKH LWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATA+AKG
Sbjct: 241 IRIEVPPFEKKHDLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAIAKG 300

Query: 301 LSAPLQSCPSVFVPGEDELKQGRADGYSFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAI 360
           LSAPLQSCP+ FVPGE+ELK GR DGY FGFLHVKAIDDAGHDKATIFKVKGMEAVDRAI
Sbjct: 301 LSAPLQSCPNAFVPGENELKPGRTDGYDFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAI 360

Query: 361 GQLAKLLWKAESTGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTICRLKDFVDAVGGESVI 420
           GQLAKLLWKAES+G FQYYLCVTGDHSTPVEYGDHSFEPVPFTICRLKDFV A+GGESVI
Sbjct: 361 GQLAKLLWKAESSGKFQYYLCVTGDHSTPVEYGDHSFEPVPFTICRLKDFVGAMGGESVI 420

Query: 421 ASISLEPFPLPTIVPGEDLDWREIQKVEEERSKNSQACGGDSVCEFDEIAVAKGCLGRFP 480
           A I L+PFPLPT V  E+LDW     + EERSK+SQA GGDSVCEFDEI+ AKGCLGRFP
Sbjct: 421 AGIDLDPFPLPTTVASENLDW-----ISEERSKDSQAFGGDSVCEFDEISAAKGCLGRFP 480

Query: 481 GGEMMGIIKRFLQLDA 497
           GGEMMGIIKRFL+LDA
Sbjct: 481 GGEMMGIIKRFLKLDA 491

[Close]

BLAST of Lag0006427 vs. TAIR 10
Match: AT3G30841.1 (Cofactor-independent phosphoglycerate mutase )

HSP 1 Score: 811.2 bits (2094), Expect = 4.5e-235
Identity = 393/491 (80.04%), Postives = 440/491 (89.61%), Query Frame = 0


Query: 8   KKRVAFVLIDGLGDVSLPKFGFKTPLQVAKVPNLDAIASAGVNGLMDPVEVGLGCGSDTA 67
           KKRVAFVLIDGLGDV++PK G+KTPLQ A +PNLDAIASAG+NGLMDPVEVGLGCGSDTA
Sbjct: 9   KKRVAFVLIDGLGDVTIPKLGYKTPLQAANIPNLDAIASAGINGLMDPVEVGLGCGSDTA 68

Query: 68  HLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRRADRHFEE 127
           HLSL+GYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEK+G+V SRRADRHFEE
Sbjct: 69  HLSLMGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKSGVVVSRRADRHFEE 128

Query: 128 EGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKDNRLLLQA 187
           EGPILCAALD +K+ SFPEYEVRVRYATEHRCGVVVKGP LSGNISGTDPLKDNRLLL+A
Sbjct: 129 EGPILCAALDGLKLQSFPEYEVRVRYATEHRCGVVVKGPRLSGNISGTDPLKDNRLLLEA 188

Query: 188 EALDDSDEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANIVLLRGCGIRIEVPP 247
           + LD+S+EA  TA VVNELS+EISRILVSHP+NA+RAA+GKNIAN+VLLRGCGIRIEVPP
Sbjct: 189 KPLDESEEATHTAKVVNELSREISRILVSHPVNAERAAQGKNIANLVLLRGCGIRIEVPP 248

Query: 248 FEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAMAKGLSAPLQS 307
           F +KHGLWPCMVAPTKIIAGLG+SLGIDIL+APGATGDYRTLLTSKATA+A  LSAPL  
Sbjct: 249 FHEKHGLWPCMVAPTKIIAGLGMSLGIDILEAPGATGDYRTLLTSKATAIANALSAPLNP 308

Query: 308 CPSVFVPGEDELKQGRADGYSFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAIGQLAKLL 367
           CP+VFVPGED  K GR+DGY FGFLH+KAIDDAGHDKAT+FKV+G+EAVD+AI QLAKLL
Sbjct: 309 CPNVFVPGEDGHKPGRSDGYDFGFLHIKAIDDAGHDKATMFKVRGLEAVDKAIRQLAKLL 368

Query: 368 WKAESTGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTICRLKDFVDAVGGESVIASISLEP 427
           W+AES+ ++QY+LCVTGDHSTPVEYGDHSFEPVPFT+CRL+DFV AVGGES +   SL+P
Sbjct: 369 WQAESSTHYQYFLCVTGDHSTPVEYGDHSFEPVPFTMCRLRDFVSAVGGESAVLETSLDP 428

Query: 428 FPLPTIV-PGEDLDWREIQKVEEE--RSKNSQACGGDSVCEFDEIAVAKGCLGRFPGGEM 487
           FPLPT+V  GED     + K EEE  R +   A GGD V E +E+A A+GCLGRF GGEM
Sbjct: 429 FPLPTVVESGED-----VTKQEEESGRREAPLAIGGDLVAELNEVAAARGCLGRFHGGEM 488

Query: 488 MGIIKRFLQLD 496
           MG+I  FL L+
Sbjct: 489 MGVITNFLNLE 494

[Close]

BLAST of Lag0006427 vs. TAIR 10
Match: AT4G09520.1 (Cofactor-independent phosphoglycerate mutase )

HSP 1 Score: 801.6 bits (2069), Expect = 3.6e-232
Identity = 388/493 (78.70%), Postives = 437/493 (88.64%), Query Frame = 0


Query: 5   QQPKKRVAFVLIDGLGDVSLPKFGFKTPLQVAKVPNLDAIASAGVNGLMDPVEVGLGCGS 64
           ++ +KRVAF+LIDGLGDVS+P+ G+KTPLQ A++PNLDAIASAG+NGLMDPVEVGLGCGS
Sbjct: 2   EEVQKRVAFILIDGLGDVSIPRLGYKTPLQAAEIPNLDAIASAGINGLMDPVEVGLGCGS 61

Query: 65  DTAHLSLLGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEKTGIVTSRRADRH 124
           DTAHLSL+GYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDE +G+V SRRADRH
Sbjct: 62  DTAHLSLMGYDPRVYYRGRGAFESMGAGLAMSPGDIAFKSNFATLDEDSGVVVSRRADRH 121

Query: 125 FEEEGPILCAALDRMKIPSFPEYEVRVRYATEHRCGVVVKGPNLSGNISGTDPLKDNRLL 184
           FEEEGPILCAALD MK+PSFPEYEVRVRYATEHRCGVVVKGP LSGNISGTDPLKDNRLL
Sbjct: 122 FEEEGPILCAALDGMKLPSFPEYEVRVRYATEHRCGVVVKGPKLSGNISGTDPLKDNRLL 181

Query: 185 LQAEALDDSDEAKLTAAVVNELSKEISRILVSHPINAKRAAEGKNIANIVLLRGCGIRIE 244
           L+A+ LD+S+EA  TA VVNELS+EISRILVSHP+N KRAA+GKNIAN+VLLRGCGIRIE
Sbjct: 182 LEAKPLDESEEANHTAKVVNELSREISRILVSHPVNVKRAAQGKNIANLVLLRGCGIRIE 241

Query: 245 VPPFEKKHGLWPCMVAPTKIIAGLGLSLGIDILDAPGATGDYRTLLTSKATAMAKGLSAP 304
           VPPF +KHGLWPCMVAPTKIIAGLG+SLGIDIL+A GATGDYRTLLTSKA A+A  LSAP
Sbjct: 242 VPPFHEKHGLWPCMVAPTKIIAGLGMSLGIDILEALGATGDYRTLLTSKAIAIANALSAP 301

Query: 305 LQSCPSVFVPGEDELKQGRADGYSFGFLHVKAIDDAGHDKATIFKVKGMEAVDRAIGQLA 364
           L  CP+VF P ED  K GR+DGY FGFLH+KAIDDAGHDKAT+FKV+G+EAVD+AI QLA
Sbjct: 302 LNPCPNVFEPAEDGHKPGRSDGYDFGFLHIKAIDDAGHDKATLFKVRGLEAVDKAIRQLA 361

Query: 365 KLLWKAESTGNFQYYLCVTGDHSTPVEYGDHSFEPVPFTICRLKDFVDAVGGESVIASIS 424
           KLLW+AES+ N+QY+LCVTGDHSTPVEYGDHSFEPVPFT+CRLKDFV AVGGES +   S
Sbjct: 362 KLLWQAESSSNYQYFLCVTGDHSTPVEYGDHSFEPVPFTMCRLKDFVRAVGGESALLETS 421

Query: 425 LEPFPLPTIVPGEDLDWREIQKVEEE--RSKNSQACGGDSVCEFDEIAVAKGCLGRFPGG 484
           L+PFPLPT +   +     I K EE+  R + S A  GDSV E +EIA A+ CLGRF GG
Sbjct: 422 LDPFPLPTDIASSE---NVIGKEEEDCGRREASLAISGDSVSELNEIAAARRCLGRFHGG 481

Query: 485 EMMGIIKRFLQLD 496
           EMMG+I +FL+LD
Sbjct: 482 EMMGVITKFLKLD 491

The following BLAST results are available for this feature:

BLAST of Lag0006427 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_038890619.1	5.5e-275	94.56	probable 2,3-bisphosphoglycerate-independent phosphoglycerate mutase [Benincasa ...	[more]
XP_011656023.1	4.6e-274	93.95	uncharacterized protein LOC101219404 [Cucumis sativus] >KGN52553.1 hypothetical ...	[more]
KAA0039399.1	2.3e-273	93.55	putative 2,3-bisphosphoglycerate-independent phosphoglycerate mutase [Cucumis me...	[more]
XP_022990600.1	1.4e-270	93.75	uncharacterized protein LOC111487437 [Cucurbita maxima] >XP_022990601.1 uncharac...	[more]
XP_022134154.1	1.3e-268	93.35	uncharacterized protein LOC111006490 [Momordica charantia] >XP_022134155.1 uncha...	[more]

BLAST of Lag0006427 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O27628	1.7e-69	37.91	2,3-bisphosphoglycerate-independent phosphoglycerate mutase 1 OS=Methanothermoba...	[more]
A4YIX4	8.4e-69	38.29	2,3-bisphosphoglycerate-independent phosphoglycerate mutase OS=Metallosphaera se...	[more]
P58813	7.9e-67	32.58	2,3-bisphosphoglycerate-independent phosphoglycerate mutase OS=Methanopyrus kand...	[more]
Q980A0	1.0e-66	38.01	2,3-bisphosphoglycerate-independent phosphoglycerate mutase OS=Saccharolobus sol...	[more]
Q975P3	1.0e-66	36.54	2,3-bisphosphoglycerate-independent phosphoglycerate mutase OS=Sulfurisphaera to...	[more]

BLAST of Lag0006427 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0KSM0	2.2e-274	93.95	Metalloenzyme domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G6433...	[more]
A0A5D3BN00	1.1e-273	93.55	Putative 2,3-bisphosphoglycerate-independent phosphoglycerate mutase OS=Cucumis ...	[more]
A0A6J1JTS4	6.8e-271	93.75	uncharacterized protein LOC111487437 OS=Cucurbita maxima OX=3661 GN=LOC111487437...	[more]
A0A6J1BXB2	6.3e-269	93.35	uncharacterized protein LOC111006490 OS=Momordica charantia OX=3673 GN=LOC111006...	[more]
A0A6J1HGY7	5.4e-268	92.34	uncharacterized protein LOC111463418 OS=Cucurbita moschata OX=3662 GN=LOC1114634...	[more]

BLAST of Lag0006427 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G30841.1	4.5e-235	80.04	Cofactor-independent phosphoglycerate mutase	[more]
AT4G09520.1	3.6e-232	78.70	Cofactor-independent phosphoglycerate mutase	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR004456	2,3-bisphosphoglycerate-independent phosphoglycerate mutase	PFAM	PF10143	PhosphMutase	coord: 45..220 e-value: 1.5E-56 score: 191.0
IPR004456	2,3-bisphosphoglycerate-independent phosphoglycerate mutase	PANTHER	PTHR31209	COFACTOR-INDEPENDENT PHOSPHOGLYCERATE MUTASE	coord: 6..495
IPR004456	2,3-bisphosphoglycerate-independent phosphoglycerate mutase	CDD	cd16011	iPGM_like	coord: 9..492 e-value: 2.56222E-160 score: 457.318
IPR042253	2,3-bisphosphoglycerate-independent phosphoglycerate mutase superfamily	GENE3D	3.30.70.2130	Metalloenzyme domain	coord: 82..184 e-value: 1.3E-28 score: 100.8
IPR017850	Alkaline-phosphatase-like, core domain superfamily	GENE3D	3.40.720.10	Alkaline Phosphatase, subunit A	coord: 185..493 e-value: 4.6E-62 score: 211.8
IPR017850	Alkaline-phosphatase-like, core domain superfamily	SUPERFAMILY	53649	Alkaline phosphatase-like	coord: 8..404
IPR006124	Metalloenzyme	PFAM	PF01676	Metalloenzyme	coord: 9..405 e-value: 6.2E-24 score: 85.1
None	No IPR available	PANTHER	PTHR31209:SF5	BNAA06G39690D PROTEIN	coord: 6..495

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0006427.1	Lag0006427.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006096	glycolytic process
molecular_function	GO:0046537	2,3-bisphosphoglycerate-independent phosphoglycerate mutase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0003824	catalytic activity