Lag0006389 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0006389
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionCCHC-type domain-containing protein
Locationchr6: 41948512 .. 41949467 (-)
RNA-Seq ExpressionLag0006389
SyntenyLag0006389
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGGAAGACAACATTGATCAGCAGTTGAGCAAGTTAAAGCTTACAGAAAAAGAAAAAGGGAAAAAGGCCAAAATCGAGGATGAGGAGTTGGAAGAATCAGACAAAAATCTGTTAAACTCCACTGTTTGCAAGATTCTATCCATGAAAACGATAAGCCCAGATGGTTTCAAAACTACGATTCCAAAGATCTGGAACCTGGTAGGGAAAATAAGAGTCGAGAAAGTGGGGAAGAACAAATTCATCTGCCACTTCCCAAACCAAAGAGAAAAAAGAAAGAATAGTAGAAGAGGGGCCTTGGATATATGACAAATCTCTAATGTTATTTACTAACGTCAAGGGAGAAAGAAAAACGGCTAACATGGATTTCAGGTACGCATCTTTTTGGGTGCATTTTCATAACCTCCCGATGGTCTTTTTTTTCCAAGAAATGGGCAAGAGTGTTGGGTAACATCATTGGAGAGTTTGAGAAAGTTGACCTGGATGAAGACAGTAGGAAGGAGGAGAGATCTTTGAGGGTCAAGATCAAAATCAACATTCACCAGCCGCTAGCAAGAGGAGCAACACTCGAAGTTGGCTCAAAAGTTGAAGAAACATGGAGTAGGGTGACGTATGAGAGATTACCAGAATTCTGTTATAGCTGTGGGAGAATAGGCCACGTTATTAAAGACTGCGATGATGAAGAAGCTGAGAAAAATTATGAACTCCAGTATGGTTCTTGGTTGAGAGAGGACCCTCTCTTCTCTCCCAAGCAAAAAGGATCAAGAGAAGGGGAAAAGCAAGAAGGAGGCAGCCAGCTGGTGGGTGCGAGAAAGGAAAGAAATCCTAAGAAAAACGAAGAGCCGGAAGGCGAGGAAGAAGAAGAGCAAGTCGGGAATGAGATGTCTACGGCGGTGCGGCAAGCGACGGAGCTGCGGTCGGAGGGCGCTGCTGAAGTTGAAAAAGAGCCGTTGTAG

mRNA sequence

ATGGAGGAAGACAACATTGATCAGCAGTTGAGCAAGTTAAAGCTTACAGAAAAAGAAAAAGGGAAAAAGGCCAAAATCGAGGATGAGGAGTTGGAAGAATCAGACAAAAATCTGTTAAACTCCACTGTTTGCAAGATTCTATCCATGAAAACGATAAGCCCAGATGGTTTCAAAACTACGATTCCAAAGATCTGGAACCTGAAATGGGCAAGAGTGTTGGGTAACATCATTGGAGAGTTTGAGAAAGTTGACCTGGATGAAGACAGTAGGAAGGAGGAGAGATCTTTGAGGGTCAAGATCAAAATCAACATTCACCAGCCGCTAGCAAGAGGAGCAACACTCGAAGTTGGCTCAAAAGTTGAAGAAACATGGAGTAGGGTGACGTATGAGAGATTACCAGAATTCTGTTATAGCTGTGGGAGAATAGGCCACGTTATTAAAGACTGCGATGATGAAGAAGCTGAGAAAAATTATGAACTCCAGTATGGTTCTTGGTTGAGAGAGGACCCTCTCTTCTCTCCCAAGCAAAAAGGATCAAGAGAAGGGGAAAAGCAAGAAGGAGGCAGCCAGCTGGTGGGTGCGAGAAAGGAAAGAAATCCTAAGAAAAACGAAGAGCCGGAAGGCGAGGAAGAAGAAGAGCAAGTCGGGAATGAGATGTCTACGGCGGTGCGGCAAGCGACGGAGCTGCGGTCGGAGGGCGCTGCTGAAGTTGAAAAAGAGCCGTTGTAG

Coding sequence (CDS)

ATGGAGGAAGACAACATTGATCAGCAGTTGAGCAAGTTAAAGCTTACAGAAAAAGAAAAAGGGAAAAAGGCCAAAATCGAGGATGAGGAGTTGGAAGAATCAGACAAAAATCTGTTAAACTCCACTGTTTGCAAGATTCTATCCATGAAAACGATAAGCCCAGATGGTTTCAAAACTACGATTCCAAAGATCTGGAACCTGAAATGGGCAAGAGTGTTGGGTAACATCATTGGAGAGTTTGAGAAAGTTGACCTGGATGAAGACAGTAGGAAGGAGGAGAGATCTTTGAGGGTCAAGATCAAAATCAACATTCACCAGCCGCTAGCAAGAGGAGCAACACTCGAAGTTGGCTCAAAAGTTGAAGAAACATGGAGTAGGGTGACGTATGAGAGATTACCAGAATTCTGTTATAGCTGTGGGAGAATAGGCCACGTTATTAAAGACTGCGATGATGAAGAAGCTGAGAAAAATTATGAACTCCAGTATGGTTCTTGGTTGAGAGAGGACCCTCTCTTCTCTCCCAAGCAAAAAGGATCAAGAGAAGGGGAAAAGCAAGAAGGAGGCAGCCAGCTGGTGGGTGCGAGAAAGGAAAGAAATCCTAAGAAAAACGAAGAGCCGGAAGGCGAGGAAGAAGAAGAGCAAGTCGGGAATGAGATGTCTACGGCGGTGCGGCAAGCGACGGAGCTGCGGTCGGAGGGCGCTGCTGAAGTTGAAAAAGAGCCGTTGTAG

Protein sequence

MEEDNIDQQLSKLKLTEKEKGKKAKIEDEELEESDKNLLNSTVCKILSMKTISPDGFKTTIPKIWNLKWARVLGNIIGEFEKVDLDEDSRKEERSLRVKIKINIHQPLARGATLEVGSKVEETWSRVTYERLPEFCYSCGRIGHVIKDCDDEEAEKNYELQYGSWLREDPLFSPKQKGSREGEKQEGGSQLVGARKERNPKKNEEPEGEEEEEQVGNEMSTAVRQATELRSEGAAEVEKEPL
Homology
BLAST of Lag0006389 vs. NCBI nr
Match: KAE8772589.1 (hypothetical protein D1007_55405 [Hordeum vulgare])

HSP 1 Score: 93.2 bits (230), Expect = 3.3e-15
Identity = 44/123 (35.77%), Postives = 74/123 (60.16%), Query Frame = 0

Query: 73  LGNIIGEFEKVDLDEDSRKEERSLRVKIKINIHQPLARGATLEVGSKVEETWSRVTYERL 132
           +G  IGE ++V+ DED +   + LRVK+K+NI +PL RG+ +++  +  ++W    YE L
Sbjct: 144 VGGAIGEVQEVETDEDGKAVGKYLRVKVKLNITKPLMRGSMVQIDERGRKSWCPFAYEYL 203

Query: 133 PEFCYSCGRIGHVIKDCDDEEAEKNYELQYGSWLR---EDPLFSPKQKGSREGEKQEGGS 192
           P FCY+CG I HV ++C + + +K+ + Q+G WLR   E      + + S  G    GG 
Sbjct: 204 PNFCYTCGVISHVEREC-NTKVKKDEKAQFGRWLRAEVEGRRTEDRGRSSGSGSTSSGGG 263

BLAST of Lag0006389 vs. NCBI nr
Match: KAG2663507.1 (hypothetical protein I3760_16G033000 [Carya illinoinensis])

HSP 1 Score: 92.8 bits (229), Expect = 4.3e-15
Identity = 51/122 (41.80%), Postives = 75/122 (61.48%), Query Frame = 0

Query: 73  LGNIIGEFEKVDLDEDSRKEERSLRVKIKINIHQPLARGATLEV-GSKVEETWSRVTYER 132
           LG+ IGE E+V++DED     RSLRVKI +++ +PLARG T+ + G KV   WS V YE+
Sbjct: 145 LGSTIGEVEEVEVDEDDVAWGRSLRVKIMLDLRKPLARGRTIVLHGVKV---WSPVKYEK 204

Query: 133 LPEFCYSCGRIGHVIKDCDDEEAEKNYELQYGSWLREDPLFSPKQKGSREGEKQEGGSQL 192
           +P FC++CGRI H    C   + +K+  +Q+GSWLR +     + +  +E    +G S  
Sbjct: 205 IPRFCFTCGRILHGSVGC---QVQKDVSIQFGSWLRAESPLKKRWEIKKELSTDKGSSSE 260

Query: 193 VG 194
            G
Sbjct: 265 AG 260

BLAST of Lag0006389 vs. NCBI nr
Match: XP_021303063.1 (uncharacterized protein LOC8083790 [Sorghum bicolor])

HSP 1 Score: 92.4 bits (228), Expect = 5.6e-15
Identity = 53/127 (41.73%), Postives = 70/127 (55.12%), Query Frame = 0

Query: 54  PDGFKTTIPKIWNLKWARVLGNIIGEFEKVDLDEDSRKEERSLRVKIKINIHQPLARGAT 113
           P GF+T           R LGN IG+F KVDLD+ +      LR+++K+++ +PL R   
Sbjct: 147 PTGFRTK-------NIGRQLGNKIGDFLKVDLDDITSGWRDFLRIRVKLDVEKPLTRIVY 206

Query: 114 LEVGSKVEETWSRVTYERLPEFCYSCGRIGHVIKDCDDEEAEKNYELQYGSWLREDPLFS 173
           + +G    E + RV YE+LP FC  CG +GHV  +C D   EK  ELQYG WL   P   
Sbjct: 207 ISLGGGKREAF-RVKYEKLPRFCAVCGFLGHVESECGDGVHEKK-ELQYGDWLIASPERK 264

Query: 174 PKQKGSR 181
            K K SR
Sbjct: 267 AKVKSSR 264

BLAST of Lag0006389 vs. NCBI nr
Match: KAF0892003.1 (hypothetical protein E2562_012483 [Oryza meyeriana var. granulata])

HSP 1 Score: 91.7 bits (226), Expect = 9.6e-15
Identity = 56/167 (33.53%), Postives = 89/167 (53.29%), Query Frame = 0

Query: 4   DNIDQQLSKLKLTEKEKGKKAKIEDEELEESDKNLLNSTVCKILSMKTISPDGFKTTIPK 63
           ++++  + ++KL   E+     +  EE+++     ++  + K+LS K   P+       +
Sbjct: 2   ESVEGMMRRMKLLVAEQKGIKIMGGEEMKKG--GCMHQAIGKLLSEKLAPPEAITKLPFR 61

Query: 64  IWNLKWARVLGNIIGEFEKVDLDEDSRKEERSLRVKIKINIHQPLARGATLEVGSKVEET 123
           + N      LG  IGEF +VD+D++     R LRVK++I+I +PL  G T+ VG   E+ 
Sbjct: 62  MMNKIARETLGGEIGEFMEVDVDDNDPMSSRYLRVKVRIDILKPLMHGVTVVVGQNEEKR 121

Query: 124 WSRVTYERLPEFCYSCGRIGHVIKDCDDEEAEKNYELQYGSWLREDP 171
           W  + YE LP+FCY CG IGH  K C  +   + Y  QYG  LR  P
Sbjct: 122 WCPLIYEFLPDFCYVCGIIGHTEKLCASQMKGEAY--QYGRELRIIP 164

BLAST of Lag0006389 vs. NCBI nr
Match: XP_028122006.1 (uncharacterized protein LOC114319195 [Camellia sinensis])

HSP 1 Score: 90.9 bits (224), Expect = 1.6e-14
Identity = 48/133 (36.09%), Postives = 77/133 (57.89%), Query Frame = 0

Query: 64  IWNLKWARVLGNIIGEFEKVDLDEDSRKEERSLRVKIKINIHQPLARGATLEVGSKVEET 123
           + N K   ++GN +G+F  +D ++      R++R+++ +++ +PL RG  L + S  E  
Sbjct: 137 LMNKKVGEIVGNAVGQFIDMDYEDGGIVWGRTMRIRVALDVRKPLRRGMKLAL-SSAEPI 196

Query: 124 WSRVTYERLPEFCYSCGRIGHVIKDCDDE----EAEKNYELQYGSWLREDPLFSPKQKGS 183
           W    YERLP +CY CGR+GH  ++CDD+    +  +   LQYG+WLR D +   K KGS
Sbjct: 197 WVDFKYERLPIYCYFCGRLGHSERECDDKLSSADGSRVDSLQYGAWLRMDNI---KSKGS 256

Query: 184 REG---EKQEGGS 190
           R     +K  GG+
Sbjct: 257 RRAGSIDKGVGGT 265

BLAST of Lag0006389 vs. ExPASy TrEMBL
Match: A0A6G1BXB4 (zf-CCHC_4 domain-containing protein OS=Oryza meyeriana var. granulata OX=110450 GN=E2562_012483 PE=4 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 4.6e-15
Identity = 56/167 (33.53%), Postives = 89/167 (53.29%), Query Frame = 0

Query: 4   DNIDQQLSKLKLTEKEKGKKAKIEDEELEESDKNLLNSTVCKILSMKTISPDGFKTTIPK 63
           ++++  + ++KL   E+     +  EE+++     ++  + K+LS K   P+       +
Sbjct: 2   ESVEGMMRRMKLLVAEQKGIKIMGGEEMKKG--GCMHQAIGKLLSEKLAPPEAITKLPFR 61

Query: 64  IWNLKWARVLGNIIGEFEKVDLDEDSRKEERSLRVKIKINIHQPLARGATLEVGSKVEET 123
           + N      LG  IGEF +VD+D++     R LRVK++I+I +PL  G T+ VG   E+ 
Sbjct: 62  MMNKIARETLGGEIGEFMEVDVDDNDPMSSRYLRVKVRIDILKPLMHGVTVVVGQNEEKR 121

Query: 124 WSRVTYERLPEFCYSCGRIGHVIKDCDDEEAEKNYELQYGSWLREDP 171
           W  + YE LP+FCY CG IGH  K C  +   + Y  QYG  LR  P
Sbjct: 122 WCPLIYEFLPDFCYVCGIIGHTEKLCASQMKGEAY--QYGRELRIIP 164

BLAST of Lag0006389 vs. ExPASy TrEMBL
Match: A0A803P119 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 90.5 bits (223), Expect = 1.0e-14
Identity = 40/106 (37.74%), Postives = 63/106 (59.43%), Query Frame = 0

Query: 70  ARVLGNIIGEFEKVDLDEDSRKEERSLRVKIKINIHQPLARGATLEVGSKVEETWSRVTY 129
           A ++G+I+GEF KVD D         +R++I +++ QPL RG  L++   V++ W  + Y
Sbjct: 472 ANIIGSIVGEFLKVDDDSLEEGWGPFMRIRISLDVTQPLLRGTLLKITGLVDDLWVILKY 531

Query: 130 ERLPEFCYSCGRIGHVIKDC----DDEEAEKNYELQYGSWLREDPL 172
           E+LP+ CY CGR+GH    C    + E+   +  L YG W++ DPL
Sbjct: 532 EKLPDICYKCGRLGHTFLRCIEFLEAEDVGGDTSLPYGPWMKGDPL 577

BLAST of Lag0006389 vs. ExPASy TrEMBL
Match: B8AYU1 (zf-CCHC_4 domain-containing protein OS=Oryza sativa subsp. indica OX=39946 GN=OsI_20101 PE=4 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 1.8e-14
Identity = 54/141 (38.30%), Postives = 76/141 (53.90%), Query Frame = 0

Query: 31  LEESDKNLLNSTVCKILSMKTISPDGFKTTIPKIWNLKWARVLGNIIGEFEKVDLDEDSR 90
           LEE D   +   V K+ S +     G       + N+   R++GN IG+  +VD DED  
Sbjct: 35  LEEKDGGKVQQAVGKLFSPRA----GVYNLPFGLMNVDTGRLIGNKIGKALEVDTDEDGS 94

Query: 91  KEERSLRVKIKINIHQPLARGATLEVGSKVEETWSRVTYERLPEFCYSCGRIGHVIKDCD 150
                LRVK+ ++  + L RG  +E  +  +E W  V YE LP FCYSCG +GHV ++CD
Sbjct: 95  AVGGYLRVKVLMDARKALIRGLMMEGVAGEKENWCGVKYEFLPNFCYSCGVLGHV-EECD 154

Query: 151 DEEAEKNYELQYGSWLREDPL 172
           D +  K  E Q+G WLR  P+
Sbjct: 155 D-KVWKEEEQQFGDWLRVLPM 169

BLAST of Lag0006389 vs. ExPASy TrEMBL
Match: A0A5C7IV40 (CCHC-type domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_001527 PE=4 SV=1)

HSP 1 Score: 88.6 bits (218), Expect = 3.9e-14
Identity = 67/232 (28.88%), Postives = 104/232 (44.83%), Query Frame = 0

Query: 1   MEEDNIDQQLSKLKLTEKEKGKKAKIEDEELEESDKNLLNSTVCKILSMKTISPDGFKTT 60
           M  D++ +    L L EKE G    ++    E+ ++ L    V KI++ K ++ D F   
Sbjct: 1   MNADDVAKLCEALSLKEKE-GPLMPLQTVMKEDGERRLALKLVGKIMANKMVNRDAFNGV 60

Query: 61  IPKIWN----------------------------------------LKWARVLGNIIGEF 120
           IPK W                                         ++W   LGN+IGE 
Sbjct: 61  IPKFWRTLEEFEIEVIEGNTFSFTFRNVEDRRHVFDGWTLEFRQSPIEW--FLGNMIGEV 120

Query: 121 EKVDLDEDSRKEERSLRVKIKINIHQPLARGATLEVGSKVEETWSRVTYERLPEFCYSCG 180
            ++D D       + +RV++ IN+ QPL R   ++V    +ET   + YERL + C+ CG
Sbjct: 121 REIDTDPSGECVGKFIRVRVVINVKQPLRRILRVDVMRDGKETTMLLHYERLLDRCFRCG 180

Query: 181 RIGHVIKDC---DDEEAEKNYELQYGSWLREDPLFSPKQKGSREGEKQEGGS 190
           R+ HVI+DC    D++  +NY L Y SWLR     +   K  +  +K+EG S
Sbjct: 181 RLDHVIRDCVVEADKKGPENYNLMYRSWLRA----ASPPKNFQARQKREGDS 225

BLAST of Lag0006389 vs. ExPASy TrEMBL
Match: A0A1Q3BJG7 (DUF4283 domain-containing protein/zf-CCHC_4 domain-containing protein OS=Cephalotus follicularis OX=3775 GN=CFOL_v3_11485 PE=4 SV=1)

HSP 1 Score: 87.8 bits (216), Expect = 6.7e-14
Identity = 39/108 (36.11%), Postives = 63/108 (58.33%), Query Frame = 0

Query: 68  KWARVLGNIIGEFEKVDLDEDSRKEERSLRVKIKINIHQPLARGATLEVGSKVEETWSRV 127
           K  +++GN IGE E +++    R+  + +RV++ I+  QPL RG    +G+  +  W   
Sbjct: 78  KVGKIVGNSIGEVEDIEVVMGRRENNQYIRVRVGIDARQPLRRGIKFHIGNS-DNIWLSF 137

Query: 128 TYERLPEFCYSCGRIGHVIKDC----DDEEAEKNYELQYGSWLREDPL 172
            YE+LP FC++CG +GH++ DC      +E  K  + QYG WLR + L
Sbjct: 138 QYEKLPNFCHACGNLGHIMNDCKTMNKGQEEWKEEDFQYGPWLRANNL 184

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAE8772589.13.3e-1535.77hypothetical protein D1007_55405 [Hordeum vulgare][more]
KAG2663507.14.3e-1541.80hypothetical protein I3760_16G033000 [Carya illinoinensis][more]
XP_021303063.15.6e-1541.73uncharacterized protein LOC8083790 [Sorghum bicolor][more]
KAF0892003.19.6e-1533.53hypothetical protein E2562_012483 [Oryza meyeriana var. granulata][more]
XP_028122006.11.6e-1436.09uncharacterized protein LOC114319195 [Camellia sinensis][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A6G1BXB44.6e-1533.53zf-CCHC_4 domain-containing protein OS=Oryza meyeriana var. granulata OX=110450 ... [more]
A0A803P1191.0e-1437.74Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
B8AYU11.8e-1438.30zf-CCHC_4 domain-containing protein OS=Oryza sativa subsp. indica OX=39946 GN=Os... [more]
A0A5C7IV403.9e-1428.88CCHC-type domain-containing protein OS=Acer yangbiense OX=1000413 GN=EZV62_00152... [more]
A0A1Q3BJG76.7e-1436.11DUF4283 domain-containing protein/zf-CCHC_4 domain-containing protein OS=Cephalo... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR025836Zinc knuckle CX2CX4HX4CPFAMPF14392zf-CCHC_4coord: 104..150
e-value: 6.6E-13
score: 48.1
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 169..242
NoneNo IPR availablePANTHERPTHR31286:SF62SUBFAMILY NOT NAMEDcoord: 68..219
IPR040256Uncharacterized protein At4g02000-likePANTHERPTHR31286GLYCINE-RICH CELL WALL STRUCTURAL PROTEIN 1.8-LIKEcoord: 68..219
IPR001878Zinc finger, CCHC-typePROSITEPS50158ZF_CCHCcoord: 136..149
score: 10.064789

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0006389.1Lag0006389.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0008270 zinc ion binding