Lag0006332 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0006332
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionReverse transcriptase domain-containing protein
Locationchr6: 41368631 .. 41370017 (+)
RNA-Seq ExpressionLag0006332
SyntenyLag0006332
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGGGGAAAGTCACTGTTGGGGGAGGGAGTTCGATGGAGGGTTGGAGATGGGCAGTCCATTAACATAAGTAAGGATAGATGGATTCCTAGAGGTTCTACGCTGAAGGTTATCTATAATCAAGGGCTAGTTCCTGAGGCTAAAGTGAATATCCTGTTGAACCCAGATAGCTCGTGGAAAAAGGAGTTGATTGAGTCCTTGTTTCGAAAGGAGGATGCGGAGGCGATTCTTGGCATCCCAAGACCGAGGAGAATGGGTCAGGACACTCTGTTGTGGCATTATGAGAAAGATGGTTGTTATACTGTGCGTAGTGGATATAAGTTGGCTGTGAGGCTGAAAAATGAAGCAGGGTGTTCAAACCAGGATGTAGTGAGGAAGTGGTGGAAGTGCATGTGGGGCTGCCAAATTCCCAATAAGATTAAGATCTTCTGCTGGAGGTTATTTCACGAGTTCATTCCCACAGCTGTTAACCTCCGAAGTAGGGGTGTAGATATTTCCCCTATGTGCTCTAGATGCAAGCTGAAAGAGGAGACCTCCTTTCATGCAATCTGGGAATGTGGGTGGGCGAAAAAGCAGTGGAGGGCCTCTCCTTTCTTCCCTATCCCGGGACCCCGATCAATCCGAAGCCCAGCTGAGTTACTATGGTGGTGTAGCACAAACTTGAATATTCGCAGTCTGGAGGGTTTTATGGTTCTTAGCTGGTGGGTGTGGAATAGGAGGAATAAGGACTTGTTTTCGGAAGGAGATGCGGGTGGAAGGGATGAGGAGGGATGGAGTTGGGTAACTGAGTATTTAGCTCATTTCAGGGGTTTCCAAGGGAGAAGGTCTGAGGGGGGGGGTTCGGATGCAAATTTCTCGATGGATTCCCCCAATTCCTCCTAATTACAAAATTAATACAGATGCGGCGACAAACAGTTATACGCAATCCAGTAGTATAGGAGCTATAATTCGGAATGAGAGAGAGGAAGTTTTACTTACCGTCGGGAGGCTGGTTCGATTTGTGCTAGATGTGGATTCGTTGGAGACTCTAGCAATCCGTGAGGGTTTGTTGGTGGCTAGAGCAGCTGGTTTTTCGAGACTGGAGGTGGAGACCGATTCTTCACGGGTTGCGGCCATGGTTAACTCGAGCCGGAGAGATCTGTCTGAGGTGGGCTCTCTGGTTGACGAGATAAAGCAGATTTTGAAGGGCATGGAGTTCTGCTCTGTGTCTTGGTGCCGCAGAGTTGCCAATCGTGTAGCTCACGCAGCGGCGAAGCTGGTTCTTGAGGAAGGAGTGCAAGGCATCTGGTTGGAGGATGTGCCGACAAGTATTGCAGAGGTTTATTTTAGTGAGGTGTTGAGCGGCCAAGCTCTAAGGGCTAGTGCCTCTTCTGTTGCCGTCTGTTAG

mRNA sequence

ATGTGGGGAAAGTCACTGTTGGGGGAGGGAGTTCGATGGAGGGTTGGAGATGGGCAGTCCATTAACATAAGTAAGGATAGATGGATTCCTAGAGGTTCTACGCTGAAGGTTATCTATAATCAAGGGCTAGTTCCTGAGGCTAAAGTGAATATCCTGTTGAACCCAGATAGCTCGTGGAAAAAGGAGTTGATTGAGTCCTTGTTTCGAAAGGAGGATGCGGAGGCGATTCTTGGCATCCCAAGACCGAGGAGAATGGGTCAGGACACTCTGTTGTGGCATTATGAGAAAGATGGTTGTTATACTGTGCGTAGTGGATATAAGTTGGCTGTGAGGCTGAAAAATGAAGCAGGGTGTTCAAACCAGGATGTAGTGAGGAAGTGGTGGAAGTGCATGTGGGGCTGCCAAATTCCCAATAAGATTAAGATCTTCTGCTGGAGGTTATTTCACGAGTTCATTCCCACAGCTGTTAACCTCCGAAGTAGGGGTGTAGATATTTCCCCTATGTGCTCTAGATGCAAGCTGAAAGAGGAGACCTCCTTTCATGCAATCTGGGAATGTGGGTGGGCGAAAAAGCAGTGGAGGGCCTCTCCTTTCTTCCCTATCCCGGGACCCCGATCAATCCGAAGCCCAGCTGAGTTACTATGGTGGTGTAGCACAAACTTGAATATTCGCAGTCTGGAGGGTTTTATGGTTCTTAGCTGGTGGGTGTGGAATAGGAGGAATAAGGACTTGTTTTCGGAAGGAGATGCGGGTGGAAGGGATGAGGAGGGATGGAGTTGGGTAACTGAGTATTTAGCTCATTTCAGGGGTTTCCAAGGGAGAAGGTCTGAGGGGGGGGATGCGGCGACAAACAGTTATACGCAATCCAGTAGTATAGGAGCTATAATTCGGAATGAGAGAGAGGAAGTTTTACTTACCGTCGGGAGGCTGGTTCGATTTGTGCTAGATGTGGATTCGTTGGAGACTCTAGCAATCCGTGAGGGTTTGTTGGTGGCTAGAGCAGCTGGTTTTTCGAGACTGGAGGTGGAGACCGATTCTTCACGGGTTGCGGCCATGGTTAACTCGAGCCGGAGAGATCTGTCTGAGGTGGGCTCTCTGGTTGACGAGATAAAGCAGATTTTGAAGGGCATGGAGTTCTGCTCTGTGTCTTGGTGCCGCAGAGTTGCCAATCGTGTAGCTCACGCAGCGGCGAAGCTGGTTCTTGAGGAAGGAGTGCAAGGCATCTGGTTGGAGGATGTGCCGACAAGTATTGCAGAGGTTTATTTTAGTGAGGTGTTGAGCGGCCAAGCTCTAAGGGCTAGTGCCTCTTCTGTTGCCGTCTGTTAG

Coding sequence (CDS)

ATGTGGGGAAAGTCACTGTTGGGGGAGGGAGTTCGATGGAGGGTTGGAGATGGGCAGTCCATTAACATAAGTAAGGATAGATGGATTCCTAGAGGTTCTACGCTGAAGGTTATCTATAATCAAGGGCTAGTTCCTGAGGCTAAAGTGAATATCCTGTTGAACCCAGATAGCTCGTGGAAAAAGGAGTTGATTGAGTCCTTGTTTCGAAAGGAGGATGCGGAGGCGATTCTTGGCATCCCAAGACCGAGGAGAATGGGTCAGGACACTCTGTTGTGGCATTATGAGAAAGATGGTTGTTATACTGTGCGTAGTGGATATAAGTTGGCTGTGAGGCTGAAAAATGAAGCAGGGTGTTCAAACCAGGATGTAGTGAGGAAGTGGTGGAAGTGCATGTGGGGCTGCCAAATTCCCAATAAGATTAAGATCTTCTGCTGGAGGTTATTTCACGAGTTCATTCCCACAGCTGTTAACCTCCGAAGTAGGGGTGTAGATATTTCCCCTATGTGCTCTAGATGCAAGCTGAAAGAGGAGACCTCCTTTCATGCAATCTGGGAATGTGGGTGGGCGAAAAAGCAGTGGAGGGCCTCTCCTTTCTTCCCTATCCCGGGACCCCGATCAATCCGAAGCCCAGCTGAGTTACTATGGTGGTGTAGCACAAACTTGAATATTCGCAGTCTGGAGGGTTTTATGGTTCTTAGCTGGTGGGTGTGGAATAGGAGGAATAAGGACTTGTTTTCGGAAGGAGATGCGGGTGGAAGGGATGAGGAGGGATGGAGTTGGGTAACTGAGTATTTAGCTCATTTCAGGGGTTTCCAAGGGAGAAGGTCTGAGGGGGGGGATGCGGCGACAAACAGTTATACGCAATCCAGTAGTATAGGAGCTATAATTCGGAATGAGAGAGAGGAAGTTTTACTTACCGTCGGGAGGCTGGTTCGATTTGTGCTAGATGTGGATTCGTTGGAGACTCTAGCAATCCGTGAGGGTTTGTTGGTGGCTAGAGCAGCTGGTTTTTCGAGACTGGAGGTGGAGACCGATTCTTCACGGGTTGCGGCCATGGTTAACTCGAGCCGGAGAGATCTGTCTGAGGTGGGCTCTCTGGTTGACGAGATAAAGCAGATTTTGAAGGGCATGGAGTTCTGCTCTGTGTCTTGGTGCCGCAGAGTTGCCAATCGTGTAGCTCACGCAGCGGCGAAGCTGGTTCTTGAGGAAGGAGTGCAAGGCATCTGGTTGGAGGATGTGCCGACAAGTATTGCAGAGGTTTATTTTAGTGAGGTGTTGAGCGGCCAAGCTCTAAGGGCTAGTGCCTCTTCTGTTGCCGTCTGTTAG

Protein sequence

MWGKSLLGEGVRWRVGDGQSINISKDRWIPRGSTLKVIYNQGLVPEAKVNILLNPDSSWKKELIESLFRKEDAEAILGIPRPRRMGQDTLLWHYEKDGCYTVRSGYKLAVRLKNEAGCSNQDVVRKWWKCMWGCQIPNKIKIFCWRLFHEFIPTAVNLRSRGVDISPMCSRCKLKEETSFHAIWECGWAKKQWRASPFFPIPGPRSIRSPAELLWWCSTNLNIRSLEGFMVLSWWVWNRRNKDLFSEGDAGGRDEEGWSWVTEYLAHFRGFQGRRSEGGDAATNSYTQSSSIGAIIRNEREEVLLTVGRLVRFVLDVDSLETLAIREGLLVARAAGFSRLEVETDSSRVAAMVNSSRRDLSEVGSLVDEIKQILKGMEFCSVSWCRRVANRVAHAAAKLVLEEGVQGIWLEDVPTSIAEVYFSEVLSGQALRASASSVAVC
Homology
BLAST of Lag0006332 vs. NCBI nr
Match: XP_023904177.1 (uncharacterized protein LOC112015942 [Quercus suber])

HSP 1 Score: 253.4 bits (646), Expect = 3.5e-63
Identity = 162/452 (35.84%), Postives = 227/452 (50.22%), Query Frame = 0

Query: 6   LLGEGVRWRVGDGQSINISKDRWIPRGSTLKVIYNQGL-VPEAKVNILLNPDS-SWKKEL 65
           +L  G  WRVGDG SI I  D+WIP+  T KV+++ G+ V E  V  L+ PDS  WK +L
Sbjct: 65  ILKAGCCWRVGDGTSIKILTDKWIPKHPTNKVLFSAGVDVGEWLVADLIEPDSHCWKHDL 124

Query: 66  IESLFRKEDAEAILGIPRPRRMGQDTLLWHYEKDGCYTVRSGYKLA---VRLKNEAGCSN 125
           I + F +EDA+AI  IP  RR   D+++W + K+G Y+V+SGY +A   +R +N A CS 
Sbjct: 125 IMNTFHREDAKAICRIPLSRRRVDDSIIWLHNKNGRYSVKSGYYVARQVLRSENWAECSK 184

Query: 126 QDVVRKWWKCMWGCQIPNKIKIFCWRLFHEFIPTAVNLRSRGVDISPMCSRCKLKEETSF 185
            +  ++ WK +W   +PNKIKIF WR     +PT +NL  R +     C  C    E   
Sbjct: 185 GNDEQQVWKALWKMNVPNKIKIFGWRACQGILPTRLNLAKRKIIAETFCPICLQSPENEM 244

Query: 186 HAIWECGWAKKQWRASPFFPIPGPRSIRSPAELLWWCSTNLNIRSLEGFMVLSWWVWNRR 245
           H IWEC  A+  W  S       P       +L  +    L    +  F+V +W +W++R
Sbjct: 245 HVIWECPVAQDVWAGSRIKLQKCPLGQPDMVQLFLYLLHRLEPEDMALFLVQAWMLWSQR 304

Query: 246 NKDLFSEGDAGGRDEEGW------SWVTEYLA---HFRGFQGRRSEGG------------ 305
           N  L      G   E GW       W+ E+          Q R + G             
Sbjct: 305 NSVL----HGGKLKEPGWLNKRDVEWLEEFAQVQDQLDVLQPRATVGNVWRPPAHPFFKM 364

Query: 306 --DAATNSYTQSSSIGAIIRNEREEVLLTVGRLVRFVLDVDSLETLAIREGLLVARAAGF 365
             DAA  + +  S  GA+IRNE  EV+  +  +   V   D  ETLA R+ +  A  AGF
Sbjct: 365 NFDAAIFNDSGRSGFGAVIRNELGEVMAALSAIGPSVSSSDEAETLACRKAMEFAVDAGF 424

Query: 366 SRLEVETDSSRVAAMVNSSRRDLSEVGSLVDEIKQILKGMEFCSVSWCRRVANRVAHAAA 425
           + L +E DS  V   ++SS  DLS VG++V +IK ++ G+   S SW  R  NRVAHA A
Sbjct: 425 NELIIEGDSINVIKALSSSSPDLSVVGNVVADIKCLISGLRRVSFSWVNRDCNRVAHALA 484

Query: 426 KLV--LEEGVQGIWLEDVPTSIAEVYFSEVLS 428
           K    LEE +   W+ED P    +  F + +S
Sbjct: 485 KFANSLEEDL--YWMEDAPPVALDALFLDSIS 510

BLAST of Lag0006332 vs. NCBI nr
Match: XP_024956542.1 (uncharacterized protein LOC112498908 [Citrus sinensis])

HSP 1 Score: 239.6 bits (610), Expect = 5.2e-59
Identity = 141/451 (31.26%), Postives = 227/451 (50.33%), Query Frame = 0

Query: 1    MWGKSLLGEGVRWRVGDGQSINISKDRWIPRGSTLKVIYNQGLVPEAKVNILLNPDSSWK 60
            +WG+ ++  G RWR+G+GQ ++I K  WIP+  T K +    +  EA V+ L+N ++ W 
Sbjct: 682  LWGRQIICGGSRWRIGNGQDVHIHKANWIPKPFTFKPVIKPTMPSEALVSELINGENCWD 741

Query: 61   KELIESLFRKEDAEAILGIPRPRRMGQDTLLWHYEKDGCYTVRSGYKLAVRLKNEAGCSN 120
            +ELI   F K DA+ I  IP PRR+ +D L+WH+ K G YTV+SGY+ A++++  A  S+
Sbjct: 742  EELIYKHFDKMDADVITQIPLPRRLREDELIWHFGKSGQYTVKSGYQRALKIRFPAMPSS 801

Query: 121  QDVVRKWWKCMWGCQIPNKIKIFCWRLFHEFIPTAVNLRSRGVDISPMCSRCKLKEETSF 180
             +  +  W  +W   +P KI+IF WR     +P+A NL  R +   P C  CK+  E  F
Sbjct: 802  SESSKNEWNIIWSLALPKKIRIFVWRAAKNLLPSAENLWKRKIVQEPTCQLCKMGIENVF 861

Query: 181  HAIWECGWAKKQWRASPFFPIPGPRSIRSPAELLWWCSTNLNIRSLEGFMVLSWWVWNRR 240
            HA+ +C  AKK WR S F+        +    LL       +   ++ F V+ W  WN R
Sbjct: 862  HALVDCKAAKKVWRLSLFYIDIQAAPGQDILSLLHGVKRMRSNADVDLFAVMLWAKWNAR 921

Query: 241  NKDLFSEGDAGGRDEEGWSWVTEYLAHFRGFQ----------GRRSE------------- 300
            N+ LF      G+ E   S V +  A    ++          G++ +             
Sbjct: 922  NQWLFK-----GKRENPQSVVAKAEAVMEAYKRVQPSADVSHGKQQKVAQLGWNPPQEGF 981

Query: 301  ---GGDAATNSYTQSSSIGAIIRNEREEVLLTVGRLVRFVLDVDSLETLAIREGLLVARA 360
                 DAATNS    + +GA+IR+E  +V  T  ++ +F   V   E  A+  GL VA+ 
Sbjct: 982  VKINTDAATNSEKNLAGLGAVIRDENGQVTATAIKVSKFHGSVAYAEAEAMEWGLQVAKD 1041

Query: 361  AGFSRLEVETDSSRVAAMVNSSRRDLSEVGSLVDEIKQILKGMEFCSVSWCRRVANRVAH 420
            A    + +E+DS  V ++VN+ +   SE+  +V EI+++ +  +  S  +  R  N +AH
Sbjct: 1042 AHVKDVIMESDSQEVVSLVNNRQGSRSEIYWVVLEIQKLKESFDHVSCVYTHRSCNAIAH 1101

Query: 421  AAAKLVLEEGVQGIWLEDVPTSIAEVYFSEV 426
            +  K+ LE+    +W    P  +     S++
Sbjct: 1102 SLVKIALEKCETVVWKGSYPLQVIFASSSDI 1127

BLAST of Lag0006332 vs. NCBI nr
Match: XP_024037590.1 (uncharacterized protein LOC112097210 [Citrus clementina])

HSP 1 Score: 237.3 bits (604), Expect = 2.6e-58
Identity = 143/452 (31.64%), Postives = 219/452 (48.45%), Query Frame = 0

Query: 1    MWGKSLLGEGVRWRVGDGQSINISKDRWIPRGSTLKVIYNQGLVPEAKVNILLNPDSSWK 60
            +WG+ +L +G RWR+G+GQ++ +  + WIPR +T K I    +  +  V  L++    W+
Sbjct: 711  VWGRQVLHKGARWRIGNGQNVLVYGNNWIPRPTTFKPISAPSMGTDTTVAELIDEKQQWR 770

Query: 61   KELIESLFRKEDAEAILGIPRPRRMGQDTLLWHYEKDGCYTVRSGYKLAVRLK--NEAGC 120
            ++LI   FR EDAEAI+ IP P+R  +D L+WHY+K G Y+V+SGY++A+R+K   +  C
Sbjct: 771  EDLILQHFRPEDAEAIMQIPLPKRPKEDQLIWHYDKKGYYSVKSGYQVAMRIKFPEDPSC 830

Query: 121  SNQDVVRKWWKCMWGCQIPNKIKIFCWRLFHEFIPTAVNLRSRGVDISPMCSRCKLKEET 180
            SN D  +  W+ +W   IP K+KIF WR  H+ +PTA NL  + V   PMC  C    ET
Sbjct: 831  SNHD--QNLWRFIWKLAIPEKVKIFLWRAAHDLLPTAENLWKKKVLQEPMCQSCHCHVET 890

Query: 181  SFHAIWECGWAKKQWRASPFF-PIPGPRSIRSPAELLWWCSTNLNIRSLEGFMVLSWWVW 240
              HA+ EC  A+K WR S     + G         L +W   +  +   E    L W +W
Sbjct: 891  VSHALVECNRARKIWRYSNLAEELRGVYRCDIVWMLQFWPRQHAKVEGAE-VAALLWAIW 950

Query: 241  NRRNKDLFSEGDAGGRDEEGWSWVTEYLAHFRGFQ------------------------- 300
              RNK LF      G+ E     V    A    F+                         
Sbjct: 951  KARNKWLFE-----GKKENPLRVVANAEAIVESFKKIRQPEMVYKTKGNAERQKQWSPPP 1010

Query: 301  -GRRSEGGDAATNSYTQSSSIGAIIRNEREEVLLTVGRLVRFVLDVDSLETLAIREGLLV 360
             G +    DAA +   Q + +G ++R+          + +R    V   E  A+  GL V
Sbjct: 1011 NGWQKVNVDAAVDVENQMAGLGVVVRDSDGNCRAAAIKSLRLPGSVAMAEATAMEWGLKV 1070

Query: 361  ARAAGFSRLEVETDSSRVAAMVNSSRRDLSEVGSLVDEIKQILKGMEFCSVSWCRRVANR 420
            A  A  +    E+DS  V  ++N     L+E+G L+ +I++ L+  +        R  N 
Sbjct: 1071 AEKAHITFGIFESDSLEVIDLINKKSSSLTEIGWLISDIQENLQNFQNFKAQHSPRDCNY 1130

Query: 421  VAHAAAKLVLEEGVQGIWLEDVPTSIAEVYFS 424
             AH+ AKL L++    IWL+++P  I  ++ S
Sbjct: 1131 AAHSLAKLALQKKETVIWLDEIPPEIMYLFLS 1154

BLAST of Lag0006332 vs. NCBI nr
Match: GAY61101.1 (hypothetical protein CUMW_207140, partial [Citrus unshiu])

HSP 1 Score: 236.5 bits (602), Expect = 4.4e-58
Identity = 140/436 (32.11%), Postives = 217/436 (49.77%), Query Frame = 0

Query: 1   MWGKSLLGEGVRWRVGDGQSINISKDRWIPRGSTLKVIYNQGLVPEAKVNILLNPDSSWK 60
           +WG+ ++  G RWR+G+GQ ++I K  WIP+  T K +    L  +A V+ L+N ++ W 
Sbjct: 23  LWGRQIICAGSRWRIGNGQDVHIHKANWIPKPFTFKPVIKPTLPSKALVSELINGENCWD 82

Query: 61  KELIESLFRKEDAEAILGIPRPRRMGQDTLLWHYEKDGCYTVRSGYKLAVRLKNEAGCSN 120
           +ELI   F K DA+ I  IP PRR  +D L+WH+ K G YTV+SGY+ A+ ++  A  S+
Sbjct: 83  EELIYRHFDKMDADVITQIPLPRRSREDELIWHFGKSGQYTVKSGYQTALNIRFPAMPSS 142

Query: 121 QDVVRKWWKCMWGCQIPNKIKIFCWRLFHEFIPTAVNLRSRGVDISPMCSRCKLKEETSF 180
            +  +  W  +W   +P KI+IF WR     +P+  NL  R +   P C  CK+  E  F
Sbjct: 143 SEFSKNEWNIIWSLALPKKIRIFVWRAAKNLLPSDENLWKRKIVQEPTCQLCKMGIENVF 202

Query: 181 HAIWECGWAKKQWRASPFFPIPGPRSIRSPAELLWWCSTNLNIRSLEGFMVLSWWVWNRR 240
           HA+ +C  AKK WR S F         +    LL       +   ++ F  + W  WN R
Sbjct: 203 HALVDCKAAKKVWRLSLFDNDIQAAPGQDILSLLHGVKRMRSNADVDLFAAMLWAKWNAR 262

Query: 241 NKDLFSEGDAGGRDEEGWSWVTEYLAHFRGF-------------------QGRRSEGGDA 300
           N+ LF      G+ E   S V +  A    +                   +G      DA
Sbjct: 263 NQWLFK-----GKRENPQSVVAKAEAVMEAYKRVQPSADKVAQLGWNPPQEGFVKINTDA 322

Query: 301 ATNSYTQSSSIGAIIRNEREEVLLTVGRLVRFVLDVDSLETLAIREGLLVARAAGFSRLE 360
           ATNS    + +GA+IR+E  +V  T  ++ +F   V   E  A+  GL VA+ A    + 
Sbjct: 323 ATNSEKNLAGLGAVIRDENGQVTATAIKVSKFHGSVAYAEAEAMEWGLQVAKDAHVKDVI 382

Query: 361 VETDSSRVAAMVNSSRRDLSEVGSLVDEIKQILKGMEFCSVSWCRRVANRVAHAAAKLVL 418
           +E+DS  V ++VN+ +   SE+  +V EI+++ +  +  S  +  R  N +AH+ AKL L
Sbjct: 383 MESDSQEVVSLVNNRQGSRSEIYWVVLEIQKLKESFDHVSCVYTHRSCNAIAHSLAKLAL 442

BLAST of Lag0006332 vs. NCBI nr
Match: ONK66393.1 (uncharacterized protein A4U43_C06F7380 [Asparagus officinalis])

HSP 1 Score: 229.2 bits (583), Expect = 7.0e-56
Identity = 146/463 (31.53%), Postives = 212/463 (45.79%), Query Frame = 0

Query: 1    MWGKSLLGEGVRWRVGDGQSINISKDRWIPRGSTLKVIYNQGLVPEAKVNILLNPDSSWK 60
            +WGK +L  G+RWRVGDG  I +  D+WIP+  T +VI +  +   A V  L+ P  +W 
Sbjct: 605  VWGKEVLQMGMRWRVGDGTQIRLKVDKWIPQPHTFRVISHNAIPENATVADLILPSRAWN 664

Query: 61   KELIESLFRKEDAEAILGIPRPRRMGQDTLLWHYEKDGCYTVRSGYKLAVRLKNE----- 120
             ELI  LF   +A  IL IP PR  G D L+WHY K+G Y+V+SGY +A + +N      
Sbjct: 665  VELIRRLFLVSEANMILSIPLPRNGGVDKLVWHYNKNGRYSVKSGYWVADQARNRREDAA 724

Query: 121  --AGCSNQDVVRKWWKCMWGCQIPNKIKIFCWRLFHEFIPTAVNLRSRGVDISPMCSRCK 180
              +   N  +  + WK +WG  +PNKIK+F WR    FIP A  L  + V +   C  CK
Sbjct: 725  SGSSVGNNSLSHQGWKRLWGLNLPNKIKVFLWRACRGFIPCADALFKQHVGVEGRCQGCK 784

Query: 181  LKEETSFHAIWECGWAKKQWRASPFFPIPGPRSIRSPAELLWWCSTNLNIRSLEGFMVLS 240
               E+  HA+W+C   KK W+ +    +      R    L    +   +   LE F +LS
Sbjct: 785  GGAESVVHALWDCKRVKKVWKLTFLHDVCTVWRERDFFYLFNHVAQVAHQNELEWFGILS 844

Query: 241  WWVWNRRNKDLFSEG--DAGGRDEEGWSWVTEYLAHFRGFQG------------------ 300
            W++W+ RNK ++     DA    E    W   +L + +G  G                  
Sbjct: 845  WFIWHDRNKRVYGATVFDAKNLLERASCWWEGFL-NAKGMVGCQDGSRQGLNDLREASLI 904

Query: 301  ---RRSEGG------DAATNSYTQSSSIGAIIRNEREEVLLTVGRLVRFVLDVDSLETLA 360
               R+   G      D A + +      GA++R+ER   +  +       L   + E LA
Sbjct: 905  RIWRKPTNGFVKLNVDGALDIHRGIYGTGAVVRDERGSCVGVLATPGTGALSPLTCEALA 964

Query: 361  IREGLLVARAAGFSRLEVETDSSRVAAMVNSSRRDLSEVGSLVDEIKQILKGMEFCSVSW 420
            +  GL      GF  + +E DS  V   +N S  DLS  G L+DE K ++     CS  +
Sbjct: 965  LVNGLHFCVQVGFKYVVIEGDSLAVINALNGSEEDLSMEGGLIDEAKFLMSFFISCSWDF 1024

Query: 421  CRRVANRVAHAAAKLVLEEGVQGIWLEDVPTSIAEVYFSEVLS 428
              RV N+ AH  A+  L       W   VP  ++ +   E  S
Sbjct: 1025 VPRVCNQAAHCVARRALSLPYPTFWWLMVPDWLSTIIDVEASS 1066

BLAST of Lag0006332 vs. ExPASy TrEMBL
Match: A0A803QQT2 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 245.7 bits (626), Expect = 3.5e-61
Identity = 139/434 (32.03%), Postives = 212/434 (48.85%), Query Frame = 0

Query: 1   MWGKSLLGEGVRWRVGDGQSINISKDRWIPRGSTLKVIYNQGLVPEAKVNILLNPDSSWK 60
           +WGK L+ +G RWRVG+G+S+ + +D W+PR  T KV     L     V  L   D  W 
Sbjct: 259 VWGKKLILKGYRWRVGNGESVRVLEDPWLPRPVTFKVYDQPSLPANLYVTDLKLADGQWD 318

Query: 61  KELIESLFRKEDAEAILGIPRPRRMGQDTLLWHYEKDGCYTVRSGYKLAVRLKNEAGCSN 120
           +  I S+F   D + ILGIP      +D +LWHY K G Y+V+SGY++A     E   SN
Sbjct: 319 EGFIRSIFNPTDVDLILGIPCSDWDFEDKILWHYSKYGEYSVKSGYRMAASFTTEQHQSN 378

Query: 121 QDVVRKWWKCMWGCQIPNKIKIFCWRLFHEFIPTAVNLRSRGVDISPMCSRCKLK-EETS 180
           +  + +WWK +W  +IP K+K F W++ H ++P  VNL  RG+  S +CSRC    +E+ 
Sbjct: 379 EHSIVQWWKKLWRLKIPPKVKHFVWKVAHNWLPANVNLAKRGIASSVVCSRCSSHVDESV 438

Query: 181 FHAIWECGWAKKQWRASPFFPIPGPRSIRSPAELLWWCSTNLNIRSLEGFMVLSWWVWNR 240
            HA+WEC  +K  WR S  +             +L   +   +   LE F+++SW +WN 
Sbjct: 439 AHALWECKASKGYWRVSGLYDDLKQMLGEDNLTMLMRIAAEWDKEKLEFFLLVSWNIWNV 498

Query: 241 RNKDLFSEGDAGGRDEEGWSWVTEYLAHFRGFQGR-RSEGG------------------D 300
           RN  +   G    + EE   W   +LA FRG  GR RS+                    D
Sbjct: 499 RNTVV--HGGYHPKPEEMIEWCGNFLADFRGDTGRERSQRSSEDSRWVPPARDQVTINVD 558

Query: 301 AATNSYTQSSSIGAIIRNEREEVLLTVGRLVRFVLDVDSLETLAIREGLLVARAAGFSRL 360
           A        S +G ++R+    VL     +++  L    LE +AI++G+ V       R 
Sbjct: 559 AGVKQGGLISGLGYVVRDAAGVVLSAAATVLQQELPPLQLELMAIKKGIQVGIQQRLQRF 618

Query: 361 EVETDSSRVAAMVNSSRRDLSEVGSLVDEIKQILKGMEFCSVSWCRRVANRVAHAAAKLV 415
            VETD  +   ++ +      ++  L++ I+ ++    F  +S+  R ANRVAHA A   
Sbjct: 619 SVETDCLQAVHLIQNKENGCRDIDGLLNHIRALISYDSFVGISFVFREANRVAHALANYA 678

BLAST of Lag0006332 vs. ExPASy TrEMBL
Match: A0A803Q1K6 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 241.1 bits (614), Expect = 8.6e-60
Identity = 133/415 (32.05%), Postives = 205/415 (49.40%), Query Frame = 0

Query: 1    MWGKSLLGEGVRWRVGDGQSINISKDRWIPRGSTLKVIYNQGLVPEAKVNILLNPDSSWK 60
            +WGK L+ +G RWRVG+G+S+ + +D W+PR  T KV     L     V  L   D  W 
Sbjct: 1214 VWGKKLILKGYRWRVGNGESVRVLEDPWLPRPVTFKVYDQPSLPANLYVTDLKMADGQWD 1273

Query: 61   KELIESLFRKEDAEAILGIPRPRRMGQDTLLWHYEKDGCYTVRSGYKLAVRLKNEAGCSN 120
            +  I S+F   DA+ ILGIP      +D +LWHY K+G Y+V+SGY++A  L  E   SN
Sbjct: 1274 EGSIRSIFNPTDADLILGIPCSDWDFEDKILWHYSKNGEYSVKSGYRMAASLTTEQHQSN 1333

Query: 121  QDVVRKWWKCMWGCQIPNKIKIFCWRLFHEFIPTAVNLRSRGVDISPMCSRCKLK-EETS 180
            +  + +WWK +W    P K+K F W++ H ++P  VNL  RG+  S +CSRC    EE+ 
Sbjct: 1334 EHSIVQWWKKLWRLNFPPKVKHFVWKVAHNWLPANVNLAKRGIASSVVCSRCSSHVEESV 1393

Query: 181  FHAIWECGWAKKQWRASPFFPIPGPRSIRSPAELLWWCSTNLNIRSLEGFMVLSWWVWNR 240
             HA+WEC  +K  WR S  +             +L   +   +   LE F+++SW +WN 
Sbjct: 1394 AHALWECKASKGYWRVSGLYDDLKLILGEDNLSMLMRIAAEWDKEKLEFFLLVSWNIWNV 1453

Query: 241  RNKDLFSEGDAGGRDEEGWSWVTEYLAHFRGFQGRRSEGGDAATNSYTQSSSIGAIIRNE 300
            RN  +   G    + EE   W   +LA+FRG  GR             +SS     +   
Sbjct: 1454 RNTVV--HGGYHPKPEEMIEWCGNFLAYFRGDTGRERS---------QRSSEDRRWVPPA 1513

Query: 301  REEVLLTVGRLVRFVLDVDSLETLAIREGLLVARAAGFSRLEVETDSSRVAAMVNSSRRD 360
            R++       L+         E +AI++G+ V       R  V+TD  +   ++ +    
Sbjct: 1514 RDQCRHVAALLLSLKNSHLGTEPMAIKKGIQVGIQQRLQRFSVQTDCLQAVHLIQNKENG 1573

Query: 361  LSEVGSLVDEIKQILKGMEFCSVSWCRRVANRVAHAAAKLVLEEGVQGIWLEDVP 415
              ++  L+++I+ ++    F  +S+  R ANRVAHA A   L      +W+  +P
Sbjct: 1574 CRDIDGLLNQIRALISYDNFVGISFVFREANRVAHALANYALVHKASAMWIGVIP 1617

BLAST of Lag0006332 vs. ExPASy TrEMBL
Match: A0A2H5Q972 (Uncharacterized protein (Fragment) OS=Citrus unshiu OX=55188 GN=CUMW_207140 PE=4 SV=1)

HSP 1 Score: 236.5 bits (602), Expect = 2.1e-58
Identity = 140/436 (32.11%), Postives = 217/436 (49.77%), Query Frame = 0

Query: 1   MWGKSLLGEGVRWRVGDGQSINISKDRWIPRGSTLKVIYNQGLVPEAKVNILLNPDSSWK 60
           +WG+ ++  G RWR+G+GQ ++I K  WIP+  T K +    L  +A V+ L+N ++ W 
Sbjct: 23  LWGRQIICAGSRWRIGNGQDVHIHKANWIPKPFTFKPVIKPTLPSKALVSELINGENCWD 82

Query: 61  KELIESLFRKEDAEAILGIPRPRRMGQDTLLWHYEKDGCYTVRSGYKLAVRLKNEAGCSN 120
           +ELI   F K DA+ I  IP PRR  +D L+WH+ K G YTV+SGY+ A+ ++  A  S+
Sbjct: 83  EELIYRHFDKMDADVITQIPLPRRSREDELIWHFGKSGQYTVKSGYQTALNIRFPAMPSS 142

Query: 121 QDVVRKWWKCMWGCQIPNKIKIFCWRLFHEFIPTAVNLRSRGVDISPMCSRCKLKEETSF 180
            +  +  W  +W   +P KI+IF WR     +P+  NL  R +   P C  CK+  E  F
Sbjct: 143 SEFSKNEWNIIWSLALPKKIRIFVWRAAKNLLPSDENLWKRKIVQEPTCQLCKMGIENVF 202

Query: 181 HAIWECGWAKKQWRASPFFPIPGPRSIRSPAELLWWCSTNLNIRSLEGFMVLSWWVWNRR 240
           HA+ +C  AKK WR S F         +    LL       +   ++ F  + W  WN R
Sbjct: 203 HALVDCKAAKKVWRLSLFDNDIQAAPGQDILSLLHGVKRMRSNADVDLFAAMLWAKWNAR 262

Query: 241 NKDLFSEGDAGGRDEEGWSWVTEYLAHFRGF-------------------QGRRSEGGDA 300
           N+ LF      G+ E   S V +  A    +                   +G      DA
Sbjct: 263 NQWLFK-----GKRENPQSVVAKAEAVMEAYKRVQPSADKVAQLGWNPPQEGFVKINTDA 322

Query: 301 ATNSYTQSSSIGAIIRNEREEVLLTVGRLVRFVLDVDSLETLAIREGLLVARAAGFSRLE 360
           ATNS    + +GA+IR+E  +V  T  ++ +F   V   E  A+  GL VA+ A    + 
Sbjct: 323 ATNSEKNLAGLGAVIRDENGQVTATAIKVSKFHGSVAYAEAEAMEWGLQVAKDAHVKDVI 382

Query: 361 VETDSSRVAAMVNSSRRDLSEVGSLVDEIKQILKGMEFCSVSWCRRVANRVAHAAAKLVL 418
           +E+DS  V ++VN+ +   SE+  +V EI+++ +  +  S  +  R  N +AH+ AKL L
Sbjct: 383 MESDSQEVVSLVNNRQGSRSEIYWVVLEIQKLKESFDHVSCVYTHRSCNAIAHSLAKLAL 442

BLAST of Lag0006332 vs. ExPASy TrEMBL
Match: A0A803QQ69 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 1.8e-57
Identity = 137/450 (30.44%), Postives = 221/450 (49.11%), Query Frame = 0

Query: 1    MWGKSLLGEGVRWRVGDGQSINISKDRWIPRGSTLKVIYNQGLVPEAKVNILLNPDSSWK 60
            +WG+ L+ +G RWRV +G  + +++DRWIPRG+   +     + P   VN LL+   +W+
Sbjct: 1335 LWGRELIQKGSRWRVEEGSRVRVNEDRWIPRGAPFTLRTPALVSPNTYVNTLLDDSGNWR 1394

Query: 61   KELIESLFRKEDAEAILGIPRPRRMGQDTLLWHYEKDGCYTVRSGYKLAVRLKNEAGCSN 120
             E +E +  K+D   ++GI   R  G+D L+WHY  +G YTV SGY L    K +A  SN
Sbjct: 1395 IESLEKIMHKDDIPWVVGIQTRRDRGEDALMWHYTVNGDYTVASGYNLIQTEKQKAETSN 1454

Query: 121  QDVVRKWWKCMWGCQIPNKIKIFCWRLFHEFIPTAVNLRSRGVDISPMCSRCKLKEETSF 180
            + + R WWK +W   I  K+K F WR+ H ++P+   L  RG+ +  +CS C  +EET  
Sbjct: 1455 KSIARGWWKSVWQSSITPKMKNFVWRVCHNWLPSKSELVKRGIQLDSICSGCWNREETIG 1514

Query: 181  HAIWECGWAKKQWRASPFFPIPGPRSIRSPAELL---WWCSTNLNIRSLEGFMVLSWWVW 240
            HA+W+C   K  W+ + F+ +  P+ I    +LL    +  T  +    E F+ LSW VW
Sbjct: 1515 HALWKCPRLKVVWQTAGFWHL-FPKDIEFMTDLLEFFIYMKTKCSTEEFEIFLGLSWLVW 1574

Query: 241  NRRNKDLFSEGDAGGRDEEGW-SWVTEYLAHF----------RGFQGRRS---------- 300
            N+RN+ +F        D E W  W  +Y              +   G+RS          
Sbjct: 1575 NQRNQRIFQNKKT---DFESWIPWAIDYTKSLLQRDINTKNQQTIHGKRSWTAPTAGTFM 1634

Query: 301  EGGDAATNSYTQSSSIGAIIRNEREEVLLTVGRLVRFVLDVDSLETLAIREGLLVARAAG 360
               DA+  S  Q  S+ A+IRN   +++    +     + V + E LA++ GLL+ +   
Sbjct: 1635 VNCDASVVSNLQGYSLAAVIRNAEGKLVAAEVKFKSGFVSVITAELLAVKLGLLLVQKMQ 1694

Query: 361  FSRLEVETDSSRVAAMVNSSRRDLSEVGSLVDEI--KQILKGMEFCSVSWCRRVANRVAH 420
                 V +D+      ++S     ++ GS + EI    +L G +     +  R  N+VAH
Sbjct: 1695 AKPFLVCSDNVTAINHLHSKTIPTADWGSQIKEILFSNVLDGCK--GFIFSSRDCNKVAH 1754

Query: 421  AAAKLVLEEGVQGIWLEDVPTSIAEVYFSE 425
            + AK  ++      W E +P+  A +  +E
Sbjct: 1755 SLAKWAVDNRCNSFWSEGIPSCAAALLNAE 1778

BLAST of Lag0006332 vs. ExPASy TrEMBL
Match: A0A5P1EL44 (Uncharacterized protein OS=Asparagus officinalis OX=4686 GN=A4U43_C06F7380 PE=4 SV=1)

HSP 1 Score: 229.2 bits (583), Expect = 3.4e-56
Identity = 146/463 (31.53%), Postives = 212/463 (45.79%), Query Frame = 0

Query: 1    MWGKSLLGEGVRWRVGDGQSINISKDRWIPRGSTLKVIYNQGLVPEAKVNILLNPDSSWK 60
            +WGK +L  G+RWRVGDG  I +  D+WIP+  T +VI +  +   A V  L+ P  +W 
Sbjct: 605  VWGKEVLQMGMRWRVGDGTQIRLKVDKWIPQPHTFRVISHNAIPENATVADLILPSRAWN 664

Query: 61   KELIESLFRKEDAEAILGIPRPRRMGQDTLLWHYEKDGCYTVRSGYKLAVRLKNE----- 120
             ELI  LF   +A  IL IP PR  G D L+WHY K+G Y+V+SGY +A + +N      
Sbjct: 665  VELIRRLFLVSEANMILSIPLPRNGGVDKLVWHYNKNGRYSVKSGYWVADQARNRREDAA 724

Query: 121  --AGCSNQDVVRKWWKCMWGCQIPNKIKIFCWRLFHEFIPTAVNLRSRGVDISPMCSRCK 180
              +   N  +  + WK +WG  +PNKIK+F WR    FIP A  L  + V +   C  CK
Sbjct: 725  SGSSVGNNSLSHQGWKRLWGLNLPNKIKVFLWRACRGFIPCADALFKQHVGVEGRCQGCK 784

Query: 181  LKEETSFHAIWECGWAKKQWRASPFFPIPGPRSIRSPAELLWWCSTNLNIRSLEGFMVLS 240
               E+  HA+W+C   KK W+ +    +      R    L    +   +   LE F +LS
Sbjct: 785  GGAESVVHALWDCKRVKKVWKLTFLHDVCTVWRERDFFYLFNHVAQVAHQNELEWFGILS 844

Query: 241  WWVWNRRNKDLFSEG--DAGGRDEEGWSWVTEYLAHFRGFQG------------------ 300
            W++W+ RNK ++     DA    E    W   +L + +G  G                  
Sbjct: 845  WFIWHDRNKRVYGATVFDAKNLLERASCWWEGFL-NAKGMVGCQDGSRQGLNDLREASLI 904

Query: 301  ---RRSEGG------DAATNSYTQSSSIGAIIRNEREEVLLTVGRLVRFVLDVDSLETLA 360
               R+   G      D A + +      GA++R+ER   +  +       L   + E LA
Sbjct: 905  RIWRKPTNGFVKLNVDGALDIHRGIYGTGAVVRDERGSCVGVLATPGTGALSPLTCEALA 964

Query: 361  IREGLLVARAAGFSRLEVETDSSRVAAMVNSSRRDLSEVGSLVDEIKQILKGMEFCSVSW 420
            +  GL      GF  + +E DS  V   +N S  DLS  G L+DE K ++     CS  +
Sbjct: 965  LVNGLHFCVQVGFKYVVIEGDSLAVINALNGSEEDLSMEGGLIDEAKFLMSFFISCSWDF 1024

Query: 421  CRRVANRVAHAAAKLVLEEGVQGIWLEDVPTSIAEVYFSEVLS 428
              RV N+ AH  A+  L       W   VP  ++ +   E  S
Sbjct: 1025 VPRVCNQAAHCVARRALSLPYPTFWWLMVPDWLSTIIDVEASS 1066

BLAST of Lag0006332 vs. TAIR 10
Match: AT3G09510.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 90.5 bits (223), Expect = 3.6e-18
Identity = 102/436 (23.39%), Postives = 162/436 (37.16%), Query Frame = 0

Query: 3   GKSLLGEGVRWRVGDGQSINISKDRWI----PRGSTLKVIYNQGLVPEAKVNILLNPDSS 62
           G +LL +G R  +GDGQ+I I  D  +    PR    +  Y      E  +N L     S
Sbjct: 30  GIALLKKGTRHLIGDGQNIRIGLDNIVDSHPPRPLNTEETYK-----EMTINNLFERKGS 89

Query: 63  ---WKKELIESLFRKEDAEAILGIPRPRRMGQDTLLWHYEKDGCYTVRSGYKLAVR--LK 122
              W    I     + D   I  I   +    D ++W+Y   G YTVRSGY L       
Sbjct: 90  YYFWDDSKISQFVDQSDHGFIHRIYLAKSKKPDKIIWNYNTTGEYTVRSGYWLLTHDPST 149

Query: 123 NEAGCSNQDVVRKWWKCMWGCQIPNKIKIFCWRLFHEFIPTAVNLRSRGVDISPMCSRCK 182
           N    +           +W   I  K+K F WR   + + T   L +RG+ I P C RC 
Sbjct: 150 NIPAINPPHGSIDLKTRIWNLPIMPKLKHFLWRALSQALATTERLTTRGMRIDPSCPRCH 209

Query: 183 LKEETSFHAIWECGWAKKQWRASPFFPIPGPRSIRSPAELLWWCSTNLNIRSLEGF---- 242
            + E+  HA++ C +A   WR S    I          E +      +   ++  F    
Sbjct: 210 RENESINHALFTCPFATMAWRLSDSSLIRNQLMSNDFEENISNILNFVQDTTMSDFHKLL 269

Query: 243 -MVLSWWVWNRRNKDLFSEGDAG------GRDEEGWSWVTEYLAHFRGFQGRRSEGG--- 302
            + L W +W  RN  +F++              E   W+    +H +     R       
Sbjct: 270 PVWLIWRIWKARNNVVFNKFRESPSKTVLSAKAETHDWLNATQSHKKTPSPTRQIAENKI 329

Query: 303 --------------DAATNSYTQSSSIGAIIRNEREEVLLTVGRLVRFVLDVDSLETLAI 362
                         DA  +     ++ G IIRN     +      +    +    ET A+
Sbjct: 330 EWRNPPATYVKCNFDAGFDVQKLEATGGWIIRNHYGTPISWGSMKLAHTSNPLEAETKAL 389

Query: 363 REGLLVARAAGFSRLEVETDSSRVAAMVNSSRRDLSEVGSLVDEIKQI-LKGMEFCSV-- 399
              L      G++++ +E D   +  ++N     +S   SL + ++ I     +F S+  
Sbjct: 390 LAALQQTWIRGYTQVFMEGDCQTLINLING----ISFHSSLANHLEDISFWANKFASIQF 449

BLAST of Lag0006332 vs. TAIR 10
Match: AT2G02650.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 81.3 bits (199), Expect = 2.2e-15
Identity = 81/337 (24.04%), Postives = 128/337 (37.98%), Query Frame = 0

Query: 102 VRSGYKLAVR--LKNEAGCSNQDVVRKWWKCMWGCQIPNKIKIFCWRLFHEFIPTAVNLR 161
           +RSGY +A    L  E          +  + +W   +  KIK F WR     + T   LR
Sbjct: 6   LRSGYWVATHEDLLEEEAIQPPPGSTEVKQAIWKLHVAPKIKHFLWRCVTGALATNTRLR 65

Query: 162 SRGVDISPMCSRCKLKEETSFHAIWECGWAKKQWRASPFF---PIPGPRSIRSPAELLWW 221
           SR +D  P+C RC ++EET  H ++ C + +  WR++          P S       L  
Sbjct: 66  SRNIDADPICQRCCIEEETIHHIMFNCPYTQSVWRSANIIIGNQWGPPSSFEDNLNRLIQ 125

Query: 222 CSTNLNIRSLEGFMV--LSWWVWNRRNKDLFS--------EGDAGGRDEEGWSWVTEYLA 281
            S      SL+ F+   + W +W  RN  LF         E   G +D   W    E   
Sbjct: 126 LSKTQTTNSLDRFLPFWIMWRLWKSRNVFLFQQKCQSPDYEARKGIQDATEWLNANETTE 185

Query: 282 HF-----------------------RGFQGRRSEGGDAATNSYTQSSSIGAIIRNEREEV 341
           +                         G+     + G    + YT+S   G  IR     +
Sbjct: 186 NTNVHVATNPIQTSRRDSSQWNPPPEGWVKCNFDSGYTQGSPYTRS---GWTIRECNGHI 245

Query: 342 LLTVGRLVRFVLDVDSLETLAIREGLLVARAAGFSRLEVETDSSRVAAMVNSSRRDLSEV 401
           +L     ++        E L     L V  A G   +  E+DS  +  ++N+   D S +
Sbjct: 246 VLCGNAKLQSSTCSLHAEALGFLHALQVIWAHGLRYVWFESDSKSLVTLINNG-EDHSLL 305

BLAST of Lag0006332 vs. TAIR 10
Match: AT3G25270.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 65.1 bits (157), Expect = 1.6e-10
Identity = 72/303 (23.76%), Postives = 123/303 (40.59%), Query Frame = 0

Query: 131 MWGCQIPNKIKIFCWRLFHEFIPTAVNLRSRGVDISPMCSRCKLKEETSFHAIWECGWAK 190
           +W  +   KIK F W+L    + T  NL+ R +   P C RC  ++ETS H  ++C +A+
Sbjct: 18  IWKLKTAPKIKHFLWKLLSGALATGDNLKRRHIRNHPQCHRCCQEDETSQHLFFDCFYAQ 77

Query: 191 KQWRASPFFPIPGPRSIRSPAE-----LLWWCSTNLNIRSLEGFMVLSWWVWNRRNKDLF 250
           + WRAS   P    R+     E     LL  C  N   +     + + W +W  RN+ +F
Sbjct: 78  QVWRASG-IPHQELRTTGITMETKMELLLSSCLANRQPQLFNLAIWILWRLWKSRNQLVF 137

Query: 251 SEGDAGGR--------DEEGWSWVTEYL----------AHFRGFQGRR----------SE 310
            +     +        D + W     Y+           H +    R             
Sbjct: 138 QQKSISWQNTLQRARNDVQEWEDTNTYVQSLNQQVHSSRHQQPTMARTKWQRPPSTWIKY 197

Query: 311 GGDAATNSYTQSSSIGAIIRNEREEVLLTVGRLVRFVLDVDSLET--LAIREGLLVARAA 370
             D A N  T+++  G ++R+E   V +  G+ +      DSLE+   A+   +  A + 
Sbjct: 198 NYDGAFNHQTRNAKAGWLMRDE-NGVYMGSGQAIGSTTS-DSLESEFQALIIAMQHAWSQ 257

Query: 371 GFSRLEVETDSSRVAAMVNSSRRDLSEVGSLVDEIKQILKGMEFCSVSWCRRVANRVAHA 399
           G+ ++  E DS +V  ++N+ + +     + + E +   K  E     W  R  N+ A  
Sbjct: 258 GYRKVIFEGDSKQVEELMNNEKLNFGRF-NWIREGRFWQKRFEEAVFKWVPRTNNQPADI 316

BLAST of Lag0006332 vs. TAIR 10
Match: AT3G26855.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 52.8 bits (125), Expect = 8.4e-07
Identity = 21/70 (30.00%), Postives = 37/70 (52.86%), Query Frame = 0

Query: 123 VVRKWWKCMWGCQIPNKIKIFCWRLFHEFIPTAVNLRSRGVDISPMCSRCKLKEETSFHA 182
           +   W   +W  +I  KIK+  W+  +  +P    L SR + I P C+RC+   ET  H 
Sbjct: 1   MTNNWIGDIWSLKISPKIKLLIWKALNNALPVGAQLLSRNISIEPFCTRCR-DFETITHI 60

Query: 183 IWECGWAKKQ 193
           ++ C +A+++
Sbjct: 61  LFNCPFAQRE 69

BLAST of Lag0006332 vs. TAIR 10
Match: AT2G22440.1 (BEST Arabidopsis thaliana protein match is: Ribonuclease H-like superfamily protein (TAIR:AT4G29090.1); Has 208 Blast hits to 191 proteins in 10 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 208; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 46.2 bits (108), Expect = 7.8e-05
Identity = 32/90 (35.56%), Postives = 45/90 (50.00%), Query Frame = 0

Query: 25  KDRWIPR--GSTLKVIYN--QGLVPEAKVNILLNPDSS-WKKELIESLFRKEDAEAILGI 84
           KD WIP       K I N    L+    VN L++ +++ WK + +++L    D   ILGI
Sbjct: 7   KDPWIPTILARPAKSILNIRDSLL---YVNDLIDQNTNLWKLDRLQALIDPVDIPLILGI 66

Query: 85  PRPRRMGQDTLLWHYEKDGCYTVRSGYKLA 110
              R    D   W + K G YTV+SGY +A
Sbjct: 67  RPSRTYLSDGFSWSHTKSGNYTVKSGYWVA 93

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_023904177.13.5e-6335.84uncharacterized protein LOC112015942 [Quercus suber][more]
XP_024956542.15.2e-5931.26uncharacterized protein LOC112498908 [Citrus sinensis][more]
XP_024037590.12.6e-5831.64uncharacterized protein LOC112097210 [Citrus clementina][more]
GAY61101.14.4e-5832.11hypothetical protein CUMW_207140, partial [Citrus unshiu][more]
ONK66393.17.0e-5631.53uncharacterized protein A4U43_C06F7380 [Asparagus officinalis][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A803QQT23.5e-6132.03Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A803Q1K68.6e-6032.05Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A2H5Q9722.1e-5832.11Uncharacterized protein (Fragment) OS=Citrus unshiu OX=55188 GN=CUMW_207140 PE=4... [more]
A0A803QQ691.8e-5730.44Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1[more]
A0A5P1EL443.4e-5631.53Uncharacterized protein OS=Asparagus officinalis OX=4686 GN=A4U43_C06F7380 PE=4 ... [more]
Match NameE-valueIdentityDescription
AT3G09510.13.6e-1823.39Ribonuclease H-like superfamily protein [more]
AT2G02650.12.2e-1524.04Ribonuclease H-like superfamily protein [more]
AT3G25270.11.6e-1023.76Ribonuclease H-like superfamily protein [more]
AT3G26855.18.4e-0730.00RNA-directed DNA polymerase (reverse transcriptase)-related family protein [more]
AT2G22440.17.8e-0535.56BEST Arabidopsis thaliana protein match is: Ribonuclease H-like superfamily prot... [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 280..399
e-value: 1.1E-22
score: 80.2
IPR026960Reverse transcriptase zinc-binding domainPFAMPF13966zf-RVTcoord: 100..193
e-value: 4.4E-18
score: 65.8
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 279..406
e-value: 8.6E-11
score: 43.9
NoneNo IPR availablePANTHERPTHR46736FAMILY NOT NAMEDcoord: 7..371
NoneNo IPR availablePANTHERPTHR46736:SF6SUBFAMILY NOT NAMEDcoord: 7..371
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 280..398
e-value: 9.47594E-15
score: 68.4948
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 280..401

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0006332.1Lag0006332.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity