Lag0006242 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0006242
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
Descriptionsubtilisin-like protease SBT2.5
Locationchr6: 39899532 .. 39907411 (+)
RNA-Seq ExpressionLag0006242
SyntenyLag0006242
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGGTGAATTTCCAGCGCACGGTTCTTGTGTTTTTTGCGCTTCTTGTTGTTGGGAAGGCGGAAATTTACATTGTCACCATTGACGGAGAACCTATTGTGAGTTACAAAGGTGATCTCGATGGATTTGAAGCCACAGCATTGGAATCTGATGAAAAGATCGACCCTACCAGGTTTCAACTATTCAATTCTGTATTTGTTAGTGGTTGTGTGCGTTTTGTCTTTGTCATGTTCATGACTGAAGAGCTCATAATCATGGTTGAAAATTATCTTCTAATGCTATCCAGCCAGAGAATCAATTACCACAGTTGATAATTGACGGAATTTTCGAGCTTTTTCTGTCCATGTTGTGACTTTTGCATGTGGGTTTTGTATTGTGAAATGTCATTGTCCAACAGGGATTTCGGTTTATTATTTTGGGAGCTGTCAAGCTTTGGCCCCCCTTGTTTTGACTTGTCTGGTATTTGAGATCCGATTAAAGTTGAAGAAAGCTATTAGGTTTATGGATGTATTACACGTAATTATTTATAAAGTTAGGATATTTCGTTTAGCTAAAGTTGACCCAATTAGTTTTATACTCTTCTAACTGGACTTTATCACGCATTAAAATATTCTCCACTCTTCATGTTTAATAGTTTTACATATTTCTTTTTACGATAACAAAATGCATACATACCACTTGATTATCCAATTACTATTATTGATTATTGATATTCTTAAAGTAAACCTCAGGATTTCATCTTTATGCTCCCTCTTGGATAGTTTGATCAGCTTATTTTTAAAATTATTTCTCTAGAAAAGGTAAGCGTCCGTTTGGAATACATTTTAATTTAAATGTTTTTCAAGAAAGCATTTTTTTAAAAAACATTTTGTATAAAAATTGTTTAGAATTTAAACACTCATATGTTTGGTTGCATTTGTGTTTTTATACAAAGTTATTGTATATCCAAAGACTTTTTTTAAAAAATTGGTTTCAAGTGTTTCTTTCAAAATGATTTATTTATCAATCTCATTTTCTAATAAGTCATTTTTAGTGGTTGTCAAACACTAAAAATTTTTTCAAAATGATTTTTTTCTCAAAATTAAATACTTGAAAAGTTAAACCAAACACACACTAAATTCTTCTCTTGATTATTAGAATCACATGGGGGTGGAAGGGCAATATTATATCTGTAGCAAGTTGGTGAAGAAAAACACTTCTTAAAATGTATATGTTTATTTTAATTCTATAAATCATTGTGTTCATTCTATATGTGTGGAGCTCTTGGAATAAGATTTCATTATATGTCATTATGAAAATCTTTTCTTTTGGATAGCTAGATAATTGAAAATAGTGTTCTTGTAATCAATAGCTGTTAACTTTTAGTTTTGAAGGAGTTTTATTCTACTGAAAGATTTTACCAGCTTTATGTTATTGAATATTATGCATCTCATACACGGAAGTTTCTTTCAGTGAAATTGTGACATCCTATGCTCGTCACCTTGAAAATAAACATGACATGCTTCTTGGGATGTTGTTTGAGAGAGGATCCTTCAAGAAGCTCTATAGTTATAAGCATCTCATCAATGGATTTGCAGTTGACATCTCACCTGAACAGGTTAGACTTAAGAAATCTTCTTGTGTTCCCATTTGGTCTAATAATTGGCCTTTTCCCCATACAATATTAATATTTCTCTTGCATTTAATTACCCCTTACGTCTGTAGGCAGAGATTCTAAGACATACACCAATTGTAAAATCTGTTGAGAGGGATTGGAAGGTTAGGAAACTAACAACGCACACACCAGAGTTTTTGGGCCTTCCAACTGGCGTATGGCCAACTGGTGGTGGCTTTGACAGGGCTGGAGAAGACATCGTGATTGGATTTGTGGACTCTGGGATTTATCCACATCATCCAAGTTTTGCTACATATAATACCGAACCTTTTGGGCCCTGTTCGAAGTATAAAGGGAAATGTGAAGTTGACCCCGACACCAAGAAGGATTTTTGCAATGGAAAGATTATTGGAGCCCAACATTTTGCAGAAGCTGCTAAAGCAGCTGGAGCATTTAACCCAGCTATTCATTTTGCATCTCCTTTGGATGGTGATGGACATGGAAGGTTGGCCCTTCTAATAATTTTTTAAAAAAAATTTATTTTTATTTATCTTTTGTATGTATTTATTTATTTGTTTGTTTTCACTTATCATGATTATACATTAAACTATGTTTAATCCTAAGAGAGACATTTTGATACTTAATTATCGAGGATATATTGAAGTGAACATGAACCATGTGTTTTGAGGAAGTAGCCAAGAGGTAGTTTGTAAGAGTACTAAATTTTTTTAATATTCTGAAGCTCGCTTGCACTAAAAACTCTACTTAAGTCTTTGGAATTTGGTAGAAGCTTTATTTAAAATGCTTCTGTTTTGATTCTTTTTACCTTCCTTTTCTTTCTGTTTCAAAGATGCTCCACTTACACAAGAAATGAGTTGCAATCTGCTTTGCTCATTTTACTATGGACTTGAATGATATAATATATTTTTATTTACTTCATGTTTTACTGTACCAGTCATACGGCAGCAATTGCAGCTGGAAATAACGGAATCCCTGTGAGAATGCATGGCTATGAATTTGGTAAAGCTAGTGGGATGGCTCCCCGTGCTAGGTGAGGGAGATAGAAATGGCTCAATTTAAAGTAGCTGCATCATTCTCCTTCTAAATGTTTCACTTGAATTTTCTATGCATAGAATTGCTGTATATAAGGCTCTCTACAGGATTTTTGGAGGATTTGTTGCTGATGTAGTTGCAGCTATTGATCAGGTGTGTACATCCAAGGACTTCTGAAAATCTAGATTTTGGATAAAATTAAGAGATTACACCTTTAATTGCAGCTTTCTTGTTTTGGTGTTTTCATGAGTTGACTTGTTGCTTATCCTTTTTTCCTTTTGAGTAAATTTTTTCAATATCCTCTCGTTCTTATTTATTTATTTTCAAATGTAGCCAATCTTGGTAAAGTCTATGTTGGAGTTATTTCGTTGTTTTCTGTCTGCTTTTTTCTTAGCTGCCAGTTCTCTTGTATTTCCATCATGCTCTATGAAAGTTGTTTCTTTTTTTAATATAAAAAAAGTTAGAACTGTGTTTTATAAGGTTTGCCCGGAAGTTCATGGTTGTCAGCTACAGCGGGGTATTCCTTACGCTGATGCAGTATATTTTACTATCCATGCTCCTTTCTTGGGAAATAAACAGCTTGTTACATGACTATTTGTACTATTCACATCAGTATGGGTATAATATCAAATATGTGAACGTTTTTTTCCCATCATTACATTTCTCCTTTCTTTCTTTGTTCCACTGGATAGCCTAACTCTAAGAAGCACGGACACTTTAGTTTGGCTAGCGTGTCTGTGTCCGACAAGTGTTGGACACTTGGACACTCCAACACTTGTTGGACTCGTATCGAACACTTGTTAGCACAATTGATATGTGGCAAACACTAGTTGTAGGTTCATTTCAAACCATTGTCAAGTATACTAATTAGACACAATGCTTAAATGTCAAAAATAATAAATTTTAAGAGTAAAATACATTAAATTCATTTTTTCAAGCATACAAATGCATAAACTTATTGATTTTGAGTTTCCTTCTGGTATAAAAACGATATATCATATATTTTTCTAAACGTGTCCATGTCGTGTCCGTGTCCTAGATTTTAAAAAAATGATGTGTCGCCGTATCCGTGTCGTGTCGTATCCGTGCTTCTTAGGCCTAACTGATAAAAATATAGTTTGTGTAAGAACTCTAGGTTCAAACCTCATCCCCAACTTTCTTTATAAATAACAGAAATTCTTCTTTTCCTATTACTGTGATTTGAGATAATTTCCTCTAATGGTAAAATTCTGTTCGGATGGTTTGAAACAATGAACTTATGTGAACAGGCTGTACATGATGGGGTCGATATTCTCAGTCTTTCCGTTGGGCCAAATAGTCCTCCTGCAACTACCAAGATCACGTATTTAAACCCTTTTGATGCTACCCTTCTTTCAGCTGTGAAGGCTGGTGTATTTGTTGCACAGGCTGCTGGAAATGGAGGACCATTTCCTAAAACTTTAGTGTCGTATAGTCCATGGATAGCAACTGTAGCAGCTGCAATCGATGACAGAAGATACAAAAACCATCTAAAACTTGGTAATGGAAAAATTTTGGCTGGACTTGGGTTATCACGTAAGTTTCATCAACTTGACCTACTGGTACCGTATTTCAGCTTTCAAAAGAATTGTAGTTGTCTCACATATCCTAGATTGTCAAGCTTAAATGAGAGCACACGGTTTTGACATTGATTTAGCTTTATTTTTTGAAATTGTGATCGGGGAGAATATTTGAGTGACAGATTAGAGATGAAACTATCTCATTGACACCAAAAACTTATTTTGCTTGTTCGTTCAGTGAATTTAAACTTAGTACTGTTGCTTAATCTTAAAATATTTAATATTTAAGAAATTCGTAGGATGTTCAATATCTCATATAGATAGCCTCATTAAGTTTTGAACTCATGCTTCTTGGTTGAATCCAAAGGTTTTGTTTACCACCATGCTAAACCCTTTGATTGCCAATGGTGCTATTATTCATTCATATGCAGTCACAGATAAATTCCACTGGAGAAGTTTTGAATTCCTTTTTCTCTGATCCTTAAAATCCTACAATTTGTTATTTTAGCGTGTAAAGTGATAAGTTTCAAAGAAGTTGTGGGAATATATAAGATATTCTACATTGTAGAATGTAATTTAATCTTATAATTTTGTTCTATAGGTTCTTTAATTTTTTTTACTTTTTTTTTCCTTTTTAATTTTTTATTAACGTTGCATTGCAGATGTCAAAGGCCTTTCCTGCATTTTATATTTTAGCTTATATTGTAATTGATTCAAATAAGAAGATAGAAATAATGTAAATTGGTTGTTTTGTGCAGCTGCTACACATTTAAATCGAACATACACATTGGTCGCAGCTAATGACGTTCTGTTAGATTCTTCAGTAATGAAGTACAGCCCCTCTGACTGCCAAAGGCCTGAAGTTCTAAACAAACGATTGGTTGAAGGAAAAGTTTTACTTTGTGGTTATTCATTCAGTTTTGTTGTTGGTACCGCTTCAATCAAGAAGGTCTCTCAAACAGCAAAAGCCCTTGGTGCGGCTGGCTTTGTTCTTGCTGTTGAAAACATCTCTCCTGGAGCAAAATTTGACCCTGTTCCTGTTGGCATTCCTGGGATTCTCATAACTGATGTCAGCAAGTCGATGGTAATTTTTGAATCTTGGCCACTGCAAATACTGTTTCTTGCATACAAAGTTTGTTATTCTTTCGCTGGAAAATATAATATTTATGACTAATTACATATTTTGTGTCATTTTAGAATGTACAATGGTTAATTATTTTATACTCCCATATATTATTACCCCCTCCCAACGAAAAGCTTATCATCTTATAGTGTATCTTTCTCTTTGAAAATAATCAGATTTAGTTTACATATTATAATATCCATAGTATAGAATACTTGAGCTTCAATTTCATATTAAAAACAAAAAAAGACAGAATACTTGAGCTACTTTTGATATTTGGATTTTACCTTTTGCTATCTTTTTCCTTTCTTTTCCCGTCGTTTATATTCTTTATGCTTGCTTGTTCCTTCTCAACTATTGTCTCACTTACTATTTGGTGATTTCGTACTTCGGAATTTACTTTTAGGATCTTATAGACTACTACAACACCTCAACACCCAGAGATTGGACGGGTCGGGTCAAGAGCTTTGATGCTGTGGGTAGCATTGGGGATGGTTTGATGCCTTTATTATACAAATCAGCTCCTGAGGTAGCATTGTTTTCTGCTCGTGGACCCAATATTAGAGACTTTAGCTTTCAGGATGCAGACCTTCTTAAACCAGATATTCTAGCTCCTGGTTCTTTGATATGGGCTGCTTGGTCTCCAAATGGAACGGACGAGCCGAACTATGTCGGTGAGCATGATCAAATCCATTTATCTCAGTATTAATGTTCCTTTTATGGCAACCACCATGCTTGTATCAAAATTCAAACTCCGTGAATAGTATCTCTCCAACAATAAATAAATGAAAACAGAGAAAAACAGAGAAACAAAGCCAAAACGATTAATAGAATATGATGTAATTTTTTCCCCATTTTTAAGGAACAAATGATCCCGAAAGATGTAGAAACTGTTTTAATTCTTTCTCATCTTCTCAGTATTCTAGTAGCGGTTAAACTCAGTTTTGGATAGGGAAGTAACATCTCCAGGGAGAGGGATGACATACTAACATGGAACACTATCACAGACATACTAACTGTGTTCTATTACACCTTTAGTTTTACTTTGTAATTTGAAATGAAATACTCCATACTAACTGTTTACTTCTTCCAGGAGAGGGATTTGCTATGATTTCTGGAACGAGCATGGCAGCACCGCATATAGCTGGTATAGCAGCTCTTGTAAAACAGAAGCATCCGAACTGGAGTCCTGCAGCCATCAAATCAGCTTTGATGACAACATCAACAACAATGGACAGAGCAGGAAGACCTCTTAAAGCACAACAATTTTCTGAAACAGAAGCCATGAAACTGGTAACTGCAACACCTTTCGATTATGGGAGTGGTCATGTAAACCCAAGAGCAGCACTGGATCCTGGACTCATCTTTGATGCAGGTTCATTTTGCTTCCCCTATCTCAATTTGATATTTGAATCATACATTTGCTGCTGAACTAACAATCTTGTCAGTTATATTTGAACGCGAATCTTTCTCAGACTGAGGTCACTAGAAGGAAACACATCTTAATCCATTTTCTCTTGGGATAATCTAGTTATTGGTTCATGTGTTATGCTCACTTTTCTTTCTTTTTAAAAAAATCTGTTGAAACATTAACTTAGCAAGCCTATTGAGCAATGCAATCAATCATTCTGATAGGAATGTACCTAAGGTGTAATTAGGAATATTAGTAAGGGTATATTAGCAGTTAGTTAGGGAGTTGTTAGAGTATGGAGTTATAAATAGTGAGAGGGAGTAAGAAAGGGGGAAGGGAGGGAATTAAGTAGTGAGTTTACTCTTGAGTGTTCTCAAGAGAGGGAGGATCCAAGTACCTCGAACACTTGGGTTATTGTAATAATACATAGTGGTTCTATTCCGGTTCTTATCACATTCACTTGTCAAAAGATCGCTTTTTATGTATCAACATACTTTCTTGAGAGATTGAGATCTACGACGCAAAAGAACAATGAGCATGATGGGAATGGAAAGGTTCATATGAGAGTAAAAAGTGACATGTCTTTTCTTTCCTCTGAATTTTTCTAGGATGCTGATGGCAATCAAAATTCGTTTTCCATGTCTGCATATATGCATTCAGGCATAGCTTTTTCTTAATCATTTACTGCACTTGAGTAGTTTTTCGAATCTGTATGCAATGGCTCAGCTCACGTCAAATCTTGCTAAATCTCAGGTTATGAAGATTACTTGGGATTCTTATGCACGACAGCGGGCATCAACGTTCACGAGATACACAACTATACAAAGACACCTTGCAACTTCACCATGGGCCATCCCTGGAATCTCAACAGTCCATCAATCACCATCGCCCATCTTGTGGGAACTCAGACTGTTACTCGCACAGTAACAAATGTAGCTGAGGAAGAAACCTATACAATTACTGCAAGAATGGACCCTGCTGTTGCCATAGAAACAAGTCCTCCAGCAATGACTTTACGATCCGGTTCGTCGAGAAAATTTTCAGTAAGTCTCACAGCTCGATCACTGACAGGAACATATAGTTTCGGCGAGGTTCTATTGAAGGGAAGTAGGGGGCACAAGGTTAGAATACCTGTAGTAGCCATGGGATACCAACGATGA

mRNA sequence

ATGGGGGTGAATTTCCAGCGCACGGTTCTTGTGTTTTTTGCGCTTCTTGTTGTTGGGAAGGCGGAAATTTACATTGTCACCATTGACGGAGAACCTATTGTGAGTTACAAAGGTGATCTCGATGGATTTGAAGCCACAGCATTGGAATCTGATGAAAAGATCGACCCTACCAGTGAAATTGTGACATCCTATGCTCGTCACCTTGAAAATAAACATGACATGCTTCTTGGGATGTTGTTTGAGAGAGGATCCTTCAAGAAGCTCTATAGTTATAAGCATCTCATCAATGGATTTGCAGTTGACATCTCACCTGAACAGGCAGAGATTCTAAGACATACACCAATTGTAAAATCTGTTGAGAGGGATTGGAAGGTTAGGAAACTAACAACGCACACACCAGAGTTTTTGGGCCTTCCAACTGGCGTATGGCCAACTGGTGGTGGCTTTGACAGGGCTGGAGAAGACATCGTGATTGGATTTGTGGACTCTGGGATTTATCCACATCATCCAAGTTTTGCTACATATAATACCGAACCTTTTGGGCCCTGTTCGAAGTATAAAGGGAAATGTGAAGTTGACCCCGACACCAAGAAGGATTTTTGCAATGGAAAGATTATTGGAGCCCAACATTTTGCAGAAGCTGCTAAAGCAGCTGGAGCATTTAACCCAGCTATTCATTTTGCATCTCCTTTGGATGGTGATGGACATGGAAGTCATACGGCAGCAATTGCAGCTGGAAATAACGGAATCCCTGTGAGAATGCATGGCTATGAATTTGGTAAAGCTAGTGGGATGGCTCCCCGTGCTAGAATTGCTGTATATAAGGCTCTCTACAGGATTTTTGGAGGATTTGTTGCTGATGTAGTTGCAGCTATTGATCAGGCTGTACATGATGGGGTCGATATTCTCAGTCTTTCCGTTGGGCCAAATAGTCCTCCTGCAACTACCAAGATCACGTATTTAAACCCTTTTGATGCTACCCTTCTTTCAGCTGTGAAGGCTGGTGTATTTGTTGCACAGGCTGCTGGAAATGGAGGACCATTTCCTAAAACTTTAGTGTCGTATAGTCCATGGATAGCAACTGTAGCAGCTGCAATCGATGACAGAAGATACAAAAACCATCTAAAACTTGGTAATGGAAAAATTTTGGCTGGACTTGGGTTATCACCTGCTACACATTTAAATCGAACATACACATTGGTCGCAGCTAATGACGTTCTGTTAGATTCTTCAGTAATGAAGTACAGCCCCTCTGACTGCCAAAGGCCTGAAGTTCTAAACAAACGATTGGTTGAAGGAAAAGTTTTACTTTGTGGTTATTCATTCAGTTTTGTTGTTGGTACCGCTTCAATCAAGAAGGTCTCTCAAACAGCAAAAGCCCTTGGTGCGGCTGGCTTTGTTCTTGCTGTTGAAAACATCTCTCCTGGAGCAAAATTTGACCCTGTTCCTGTTGGCATTCCTGGGATTCTCATAACTGATGTCAGCAAGTCGATGGATCTTATAGACTACTACAACACCTCAACACCCAGAGATTGGACGGGTCGGGTCAAGAGCTTTGATGCTGTGGGTAGCATTGGGGATGGTTTGATGCCTTTATTATACAAATCAGCTCCTGAGGTAGCATTGTTTTCTGCTCGTGGACCCAATATTAGAGACTTTAGCTTTCAGGATGCAGACCTTCTTAAACCAGATATTCTAGCTCCTGGTTCTTTGATATGGGCTGCTTGGTCTCCAAATGGAACGGACGAGCCGAACTATGTCGGAGAGGGATTTGCTATGATTTCTGGAACGAGCATGGCAGCACCGCATATAGCTGGTATAGCAGCTCTTGTAAAACAGAAGCATCCGAACTGGAGTCCTGCAGCCATCAAATCAGCTTTGATGACAACATCAACAACAATGGACAGAGCAGGAAGACCTCTTAAAGCACAACAATTTTCTGAAACAGAAGCCATGAAACTGGTAACTGCAACACCTTTCGATTATGGGAGTGGTCATGTAAACCCAAGAGCAGCACTGGATCCTGGACTCATCTTTGATGCAGGTTATGAAGATTACTTGGGATTCTTATGCACGACAGCGGGCATCAACGTTCACGAGATACACAACTATACAAAGACACCTTGCAACTTCACCATGGGCCATCCCTGGAATCTCAACAGTCCATCAATCACCATCGCCCATCTTGTGGGAACTCAGACTGTTACTCGCACAGTAACAAATGTAGCTGAGGAAGAAACCTATACAATTACTGCAAGAATGGACCCTGCTGTTGCCATAGAAACAAGTCCTCCAGCAATGACTTTACGATCCGGTTCGTCGAGAAAATTTTCAGTAAGTCTCACAGCTCGATCACTGACAGGAACATATAGTTTCGGCGAGGTTCTATTGAAGGGAAGTAGGGGGCACAAGGTTAGAATACCTGTAGTAGCCATGGGATACCAACGATGA

Coding sequence (CDS)

ATGGGGGTGAATTTCCAGCGCACGGTTCTTGTGTTTTTTGCGCTTCTTGTTGTTGGGAAGGCGGAAATTTACATTGTCACCATTGACGGAGAACCTATTGTGAGTTACAAAGGTGATCTCGATGGATTTGAAGCCACAGCATTGGAATCTGATGAAAAGATCGACCCTACCAGTGAAATTGTGACATCCTATGCTCGTCACCTTGAAAATAAACATGACATGCTTCTTGGGATGTTGTTTGAGAGAGGATCCTTCAAGAAGCTCTATAGTTATAAGCATCTCATCAATGGATTTGCAGTTGACATCTCACCTGAACAGGCAGAGATTCTAAGACATACACCAATTGTAAAATCTGTTGAGAGGGATTGGAAGGTTAGGAAACTAACAACGCACACACCAGAGTTTTTGGGCCTTCCAACTGGCGTATGGCCAACTGGTGGTGGCTTTGACAGGGCTGGAGAAGACATCGTGATTGGATTTGTGGACTCTGGGATTTATCCACATCATCCAAGTTTTGCTACATATAATACCGAACCTTTTGGGCCCTGTTCGAAGTATAAAGGGAAATGTGAAGTTGACCCCGACACCAAGAAGGATTTTTGCAATGGAAAGATTATTGGAGCCCAACATTTTGCAGAAGCTGCTAAAGCAGCTGGAGCATTTAACCCAGCTATTCATTTTGCATCTCCTTTGGATGGTGATGGACATGGAAGTCATACGGCAGCAATTGCAGCTGGAAATAACGGAATCCCTGTGAGAATGCATGGCTATGAATTTGGTAAAGCTAGTGGGATGGCTCCCCGTGCTAGAATTGCTGTATATAAGGCTCTCTACAGGATTTTTGGAGGATTTGTTGCTGATGTAGTTGCAGCTATTGATCAGGCTGTACATGATGGGGTCGATATTCTCAGTCTTTCCGTTGGGCCAAATAGTCCTCCTGCAACTACCAAGATCACGTATTTAAACCCTTTTGATGCTACCCTTCTTTCAGCTGTGAAGGCTGGTGTATTTGTTGCACAGGCTGCTGGAAATGGAGGACCATTTCCTAAAACTTTAGTGTCGTATAGTCCATGGATAGCAACTGTAGCAGCTGCAATCGATGACAGAAGATACAAAAACCATCTAAAACTTGGTAATGGAAAAATTTTGGCTGGACTTGGGTTATCACCTGCTACACATTTAAATCGAACATACACATTGGTCGCAGCTAATGACGTTCTGTTAGATTCTTCAGTAATGAAGTACAGCCCCTCTGACTGCCAAAGGCCTGAAGTTCTAAACAAACGATTGGTTGAAGGAAAAGTTTTACTTTGTGGTTATTCATTCAGTTTTGTTGTTGGTACCGCTTCAATCAAGAAGGTCTCTCAAACAGCAAAAGCCCTTGGTGCGGCTGGCTTTGTTCTTGCTGTTGAAAACATCTCTCCTGGAGCAAAATTTGACCCTGTTCCTGTTGGCATTCCTGGGATTCTCATAACTGATGTCAGCAAGTCGATGGATCTTATAGACTACTACAACACCTCAACACCCAGAGATTGGACGGGTCGGGTCAAGAGCTTTGATGCTGTGGGTAGCATTGGGGATGGTTTGATGCCTTTATTATACAAATCAGCTCCTGAGGTAGCATTGTTTTCTGCTCGTGGACCCAATATTAGAGACTTTAGCTTTCAGGATGCAGACCTTCTTAAACCAGATATTCTAGCTCCTGGTTCTTTGATATGGGCTGCTTGGTCTCCAAATGGAACGGACGAGCCGAACTATGTCGGAGAGGGATTTGCTATGATTTCTGGAACGAGCATGGCAGCACCGCATATAGCTGGTATAGCAGCTCTTGTAAAACAGAAGCATCCGAACTGGAGTCCTGCAGCCATCAAATCAGCTTTGATGACAACATCAACAACAATGGACAGAGCAGGAAGACCTCTTAAAGCACAACAATTTTCTGAAACAGAAGCCATGAAACTGGTAACTGCAACACCTTTCGATTATGGGAGTGGTCATGTAAACCCAAGAGCAGCACTGGATCCTGGACTCATCTTTGATGCAGGTTATGAAGATTACTTGGGATTCTTATGCACGACAGCGGGCATCAACGTTCACGAGATACACAACTATACAAAGACACCTTGCAACTTCACCATGGGCCATCCCTGGAATCTCAACAGTCCATCAATCACCATCGCCCATCTTGTGGGAACTCAGACTGTTACTCGCACAGTAACAAATGTAGCTGAGGAAGAAACCTATACAATTACTGCAAGAATGGACCCTGCTGTTGCCATAGAAACAAGTCCTCCAGCAATGACTTTACGATCCGGTTCGTCGAGAAAATTTTCAGTAAGTCTCACAGCTCGATCACTGACAGGAACATATAGTTTCGGCGAGGTTCTATTGAAGGGAAGTAGGGGGCACAAGGTTAGAATACCTGTAGTAGCCATGGGATACCAACGATGA

Protein sequence

MGVNFQRTVLVFFALLVVGKAEIYIVTIDGEPIVSYKGDLDGFEATALESDEKIDPTSEIVTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDISPEQAEILRHTPIVKSVERDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPFGPCSKYKGKCEVDPDTKKDFCNGKIIGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHTAAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGVDILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIATVAAAIDDRRYKNHLKLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVMKYSPSDCQRPEVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTAKALGAAGFVLAVENISPGAKFDPVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEVALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMAAPHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLVTATPFDYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTKTPCNFTMGHPWNLNSPSITIAHLVGTQTVTRTVTNVAEEETYTITARMDPAVAIETSPPAMTLRSGSSRKFSVSLTARSLTGTYSFGEVLLKGSRGHKVRIPVVAMGYQR
Homology
BLAST of Lag0006242 vs. NCBI nr
Match: XP_038890136.1 (subtilisin-like protease SBT2.5 isoform X1 [Benincasa hispida] >XP_038890137.1 subtilisin-like protease SBT2.5 isoform X1 [Benincasa hispida] >XP_038890138.1 subtilisin-like protease SBT2.5 isoform X1 [Benincasa hispida])

HSP 1 Score: 1592.0 bits (4121), Expect = 0.0e+00
Identity = 791/816 (96.94%), Postives = 805/816 (98.65%), Query Frame = 0

Query: 1   MGVNFQRTVLVFFALLVVGKAEIYIVTIDGEPIVSYKGDLDGFEATALESDEKIDPTSEI 60
           M VNFQ +VLVF A+LVVGKAEIYIVTI+GEPIVSYKGDLDGFEATA+ESDEKIDPTSEI
Sbjct: 1   MVVNFQCSVLVFLAILVVGKAEIYIVTIEGEPIVSYKGDLDGFEATAMESDEKIDPTSEI 60

Query: 61  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDISPEQAEILRHTPIVKSVE 120
           VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDI+ EQAEILRHTPIVKSVE
Sbjct: 61  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITHEQAEILRHTPIVKSVE 120

Query: 121 RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180
           RDWKVR+LTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGI PHHPSFATYNTEPF
Sbjct: 121 RDWKVRRLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGISPHHPSFATYNTEPF 180

Query: 181 GPCSKYKGKCEVDPDTKKDFCNGKIIGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHT 240
           GPC KYKGKCEVDPDTKKDFCNGKI+GAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHT
Sbjct: 181 GPCLKYKGKCEVDPDTKKDFCNGKIVGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHT 240

Query: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300
           AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV
Sbjct: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300

Query: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360
           DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA
Sbjct: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360

Query: 361 TVAAAIDDRRYKNHLKLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVMKYSPSDC 420
           TVAAAIDDRRYKNHL LGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVMKYSPSDC
Sbjct: 361 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVMKYSPSDC 420

Query: 421 QRPEVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTAKALGAAGFVLAVENISPGAKFD 480
           Q+PEVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTAKALGAAGFVLAVENISPGAKFD
Sbjct: 421 QKPEVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTAKALGAAGFVLAVENISPGAKFD 480

Query: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 540
           PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV
Sbjct: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 540

Query: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600
           ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA
Sbjct: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600

Query: 601 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLVTATPF 660
           APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDR GRPLKAQQFSETEAMKLV+ATPF
Sbjct: 601 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLVSATPF 660

Query: 661 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTKTPCNFTMGHPWNLN 720
           DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYT +PCNFTMGHPWNLN
Sbjct: 661 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPCNFTMGHPWNLN 720

Query: 721 SPSITIAHLVGTQTVTRTVTNVAEEETYTITARMDPAVAIETSPPAMTLRSGSSRKFSVS 780
           SPSITIAHLVG+QTVTRTVTNVAEEETYTITARMDPAVAIE +PPAMTL SGSSRKFSV+
Sbjct: 721 SPSITIAHLVGSQTVTRTVTNVAEEETYTITARMDPAVAIEVNPPAMTLLSGSSRKFSVT 780

Query: 781 LTARSLTGTYSFGEVLLKGSRGHKVRIPVVAMGYQR 817
           LTARSLTGTYSFG+VLLKGSRGHKVRIPVVAMGYQR
Sbjct: 781 LTARSLTGTYSFGQVLLKGSRGHKVRIPVVAMGYQR 816

BLAST of Lag0006242 vs. NCBI nr
Match: XP_023546345.1 (subtilisin-like protease SBT2.5 [Cucurbita pepo subsp. pepo] >XP_023546347.1 subtilisin-like protease SBT2.5 [Cucurbita pepo subsp. pepo] >XP_023546348.1 subtilisin-like protease SBT2.5 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1590.5 bits (4117), Expect = 0.0e+00
Identity = 788/816 (96.57%), Postives = 806/816 (98.77%), Query Frame = 0

Query: 1   MGVNFQRTVLVFFALLVVGKAEIYIVTIDGEPIVSYKGDLDGFEATALESDEKIDPTSEI 60
           M VNFQ TVLVF  L+VVGKAEIYIVTI+GEPIVSYKGDLDGFEATA+ES+EKIDPTSEI
Sbjct: 1   MVVNFQCTVLVFLVLVVVGKAEIYIVTIEGEPIVSYKGDLDGFEATAMESNEKIDPTSEI 60

Query: 61  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDISPEQAEILRHTPIVKSVE 120
           VTSYARHLE+KHDMLLGMLFERGSFKKLYSYKHLINGFAVDI+PEQAE LRHTPIVKSVE
Sbjct: 61  VTSYARHLESKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITPEQAETLRHTPIVKSVE 120

Query: 121 RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180
           RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF
Sbjct: 121 RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180

Query: 181 GPCSKYKGKCEVDPDTKKDFCNGKIIGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHT 240
           GPC KYKGKCEVDPDTKK+FCNGKI+GAQHFAEAAKAAGAFNPAIHF SPLDGDGHGSHT
Sbjct: 181 GPCLKYKGKCEVDPDTKKEFCNGKIVGAQHFAEAAKAAGAFNPAIHFESPLDGDGHGSHT 240

Query: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300
           AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV
Sbjct: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300

Query: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360
           DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA
Sbjct: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360

Query: 361 TVAAAIDDRRYKNHLKLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVMKYSPSDC 420
           TVAAAIDDRRYKNHL LGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSV KYSPSDC
Sbjct: 361 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVSKYSPSDC 420

Query: 421 QRPEVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTAKALGAAGFVLAVENISPGAKFD 480
           QRP+VLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTA+ALGAAGFVLAVENISPG+KFD
Sbjct: 421 QRPDVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTARALGAAGFVLAVENISPGSKFD 480

Query: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 540
           PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV
Sbjct: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 540

Query: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600
           ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA
Sbjct: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600

Query: 601 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLVTATPF 660
           APHIAGI+ALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLV+ATPF
Sbjct: 601 APHIAGISALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLVSATPF 660

Query: 661 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTKTPCNFTMGHPWNLN 720
           DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHN+T +PCNFTMGHPWNLN
Sbjct: 661 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNFTNSPCNFTMGHPWNLN 720

Query: 721 SPSITIAHLVGTQTVTRTVTNVAEEETYTITARMDPAVAIETSPPAMTLRSGSSRKFSVS 780
           +PSITIAHLVGT+TVTRTVTNVAEEETYTITARMDPAVAIETSPPAMTLRSGSSRKFSV+
Sbjct: 721 TPSITIAHLVGTKTVTRTVTNVAEEETYTITARMDPAVAIETSPPAMTLRSGSSRKFSVT 780

Query: 781 LTARSLTGTYSFGEVLLKGSRGHKVRIPVVAMGYQR 817
           LTARSLTGTYSFGEVLLKGSRGHKVRIPVVAMGYQR
Sbjct: 781 LTARSLTGTYSFGEVLLKGSRGHKVRIPVVAMGYQR 816

BLAST of Lag0006242 vs. NCBI nr
Match: XP_022996483.1 (subtilisin-like protease SBT2.5 isoform X1 [Cucurbita maxima] >XP_022996484.1 subtilisin-like protease SBT2.5 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1589.7 bits (4115), Expect = 0.0e+00
Identity = 788/816 (96.57%), Postives = 805/816 (98.65%), Query Frame = 0

Query: 1   MGVNFQRTVLVFFALLVVGKAEIYIVTIDGEPIVSYKGDLDGFEATALESDEKIDPTSEI 60
           M VNFQ TVLVF  LLVVGKAEIYIVTI+GEPIVSYKGDLDGFEATA+ES+EKIDPTSEI
Sbjct: 1   MVVNFQCTVLVFLVLLVVGKAEIYIVTIEGEPIVSYKGDLDGFEATAMESNEKIDPTSEI 60

Query: 61  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDISPEQAEILRHTPIVKSVE 120
           VTSYARHLE+KHDMLLGMLFERGSFKKLYSYKHLINGFAVDI+PEQAE LRHTPIVKSVE
Sbjct: 61  VTSYARHLESKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITPEQAETLRHTPIVKSVE 120

Query: 121 RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180
            DWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF
Sbjct: 121 MDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180

Query: 181 GPCSKYKGKCEVDPDTKKDFCNGKIIGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHT 240
           GPC KYKGKCEVDPDTKK+FCNGKI+GAQHFAEAAKAAGAFNPAIHF SPLDGDGHGSHT
Sbjct: 181 GPCLKYKGKCEVDPDTKKEFCNGKIVGAQHFAEAAKAAGAFNPAIHFESPLDGDGHGSHT 240

Query: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300
           AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV
Sbjct: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300

Query: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360
           DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA
Sbjct: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360

Query: 361 TVAAAIDDRRYKNHLKLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVMKYSPSDC 420
           TVAAAIDDRRYKNHL LGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSV KYSPSDC
Sbjct: 361 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVSKYSPSDC 420

Query: 421 QRPEVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTAKALGAAGFVLAVENISPGAKFD 480
           QRP+VLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTA+ALGAAGFVLAVENISPG+KFD
Sbjct: 421 QRPDVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTARALGAAGFVLAVENISPGSKFD 480

Query: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 540
           PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV
Sbjct: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 540

Query: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600
           ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA
Sbjct: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600

Query: 601 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLVTATPF 660
           APHIAGI+ALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLV+ATPF
Sbjct: 601 APHIAGISALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLVSATPF 660

Query: 661 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTKTPCNFTMGHPWNLN 720
           DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYT +PCNFTMGHPWNLN
Sbjct: 661 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPCNFTMGHPWNLN 720

Query: 721 SPSITIAHLVGTQTVTRTVTNVAEEETYTITARMDPAVAIETSPPAMTLRSGSSRKFSVS 780
           +PSITIAHLVGT+TVTRTVTNVAEEETYTITARMDPAVAIETSPPAMTLRSGSSRKFSV+
Sbjct: 721 TPSITIAHLVGTKTVTRTVTNVAEEETYTITARMDPAVAIETSPPAMTLRSGSSRKFSVT 780

Query: 781 LTARSLTGTYSFGEVLLKGSRGHKVRIPVVAMGYQR 817
           LT+RSLTGTYSFGEVLLKGSRGHKVRIPVVAMGYQR
Sbjct: 781 LTSRSLTGTYSFGEVLLKGSRGHKVRIPVVAMGYQR 816

BLAST of Lag0006242 vs. NCBI nr
Match: XP_022929689.1 (subtilisin-like protease SBT2.5 [Cucurbita moschata] >XP_022929690.1 subtilisin-like protease SBT2.5 [Cucurbita moschata] >XP_022929691.1 subtilisin-like protease SBT2.5 [Cucurbita moschata])

HSP 1 Score: 1588.2 bits (4111), Expect = 0.0e+00
Identity = 786/816 (96.32%), Postives = 805/816 (98.65%), Query Frame = 0

Query: 1   MGVNFQRTVLVFFALLVVGKAEIYIVTIDGEPIVSYKGDLDGFEATALESDEKIDPTSEI 60
           M VNFQ TVLVF  L+VVGKAEIYIVTI+GEPIVSYKGDLDGFEATA+ES+EKIDPTSEI
Sbjct: 1   MVVNFQCTVLVFLVLVVVGKAEIYIVTIEGEPIVSYKGDLDGFEATAMESNEKIDPTSEI 60

Query: 61  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDISPEQAEILRHTPIVKSVE 120
           VTSYARHLE+KHDMLLGMLFERGSFKKLYSYKHLINGFAVDI+PEQAE LRHTPIVKSVE
Sbjct: 61  VTSYARHLESKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITPEQAETLRHTPIVKSVE 120

Query: 121 RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180
           RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF
Sbjct: 121 RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180

Query: 181 GPCSKYKGKCEVDPDTKKDFCNGKIIGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHT 240
           GPC KYKGKCEVDPDTKK+FCNGKI+GAQHFAEAAKAAGAFNPAIHF SPLDGDGHGSHT
Sbjct: 181 GPCLKYKGKCEVDPDTKKEFCNGKIVGAQHFAEAAKAAGAFNPAIHFESPLDGDGHGSHT 240

Query: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300
           AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV
Sbjct: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300

Query: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360
           DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA
Sbjct: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360

Query: 361 TVAAAIDDRRYKNHLKLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVMKYSPSDC 420
           TVAAAIDDRRYKNHL LGNGKILAGLGLSPATH NRTYTLVAANDVLLDSSV KYSPSDC
Sbjct: 361 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHFNRTYTLVAANDVLLDSSVSKYSPSDC 420

Query: 421 QRPEVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTAKALGAAGFVLAVENISPGAKFD 480
           QRP+VLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTA+ALGAAGFVLAVENISPG+KFD
Sbjct: 421 QRPDVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTARALGAAGFVLAVENISPGSKFD 480

Query: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 540
           PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV
Sbjct: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 540

Query: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600
           ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA
Sbjct: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600

Query: 601 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLVTATPF 660
           APHIAGI+ALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLV+ATPF
Sbjct: 601 APHIAGISALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLVSATPF 660

Query: 661 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTKTPCNFTMGHPWNLN 720
           DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHN+T +PCNFTMGHPWNLN
Sbjct: 661 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNHTNSPCNFTMGHPWNLN 720

Query: 721 SPSITIAHLVGTQTVTRTVTNVAEEETYTITARMDPAVAIETSPPAMTLRSGSSRKFSVS 780
           +PSITIAHLVGT+TVTRTVTNVAEEETYTITARMDPA+AIETSPPAMTLRSGSSRKFSV+
Sbjct: 721 TPSITIAHLVGTKTVTRTVTNVAEEETYTITARMDPAIAIETSPPAMTLRSGSSRKFSVT 780

Query: 781 LTARSLTGTYSFGEVLLKGSRGHKVRIPVVAMGYQR 817
           LTARSLTGTYSFGEVLLKGSRGHKVRIPVVAMGYQR
Sbjct: 781 LTARSLTGTYSFGEVLLKGSRGHKVRIPVVAMGYQR 816

BLAST of Lag0006242 vs. NCBI nr
Match: KAG6598892.1 (Subtilisin-like protease 2.5, partial [Cucurbita argyrosperma subsp. sororia] >KAG7029843.1 Subtilisin-like protease SBT2.5 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1585.9 bits (4105), Expect = 0.0e+00
Identity = 786/816 (96.32%), Postives = 805/816 (98.65%), Query Frame = 0

Query: 1   MGVNFQRTVLVFFALLVVGKAEIYIVTIDGEPIVSYKGDLDGFEATALESDEKIDPTSEI 60
           M VNFQ TVLVF  L+VVGKAEIYIVTI+GEPIVSYKGDLDGFEATA+ES+EKIDPTSEI
Sbjct: 1   MVVNFQCTVLVFLVLVVVGKAEIYIVTIEGEPIVSYKGDLDGFEATAMESNEKIDPTSEI 60

Query: 61  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDISPEQAEILRHTPIVKSVE 120
           VTSYARHLE+KHDMLLGMLFERGSFKKLYSYKHLINGFAVDI+PEQAE LRHTPIVKSVE
Sbjct: 61  VTSYARHLESKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITPEQAETLRHTPIVKSVE 120

Query: 121 RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180
           RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF
Sbjct: 121 RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180

Query: 181 GPCSKYKGKCEVDPDTKKDFCNGKIIGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHT 240
           GPCSKYKGKCEVDP TKK+FCNGKI+GAQHFAEAAKAAGAFNPAIHF SPLDGDGHGSHT
Sbjct: 181 GPCSKYKGKCEVDPHTKKEFCNGKIVGAQHFAEAAKAAGAFNPAIHFESPLDGDGHGSHT 240

Query: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300
           AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV
Sbjct: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300

Query: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360
           DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA
Sbjct: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360

Query: 361 TVAAAIDDRRYKNHLKLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVMKYSPSDC 420
           TVAAAIDDRRYKNHL LGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSV KYSPSDC
Sbjct: 361 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVSKYSPSDC 420

Query: 421 QRPEVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTAKALGAAGFVLAVENISPGAKFD 480
           QRP+VLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTA+ALGAAGFVLAVENISPG+KFD
Sbjct: 421 QRPDVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTARALGAAGFVLAVENISPGSKFD 480

Query: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 540
           PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV
Sbjct: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 540

Query: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600
           ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA
Sbjct: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600

Query: 601 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLVTATPF 660
           APHIAGI+ALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLV+ATPF
Sbjct: 601 APHIAGISALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLVSATPF 660

Query: 661 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTKTPCNFTMGHPWNLN 720
           DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINV EIHN+T +PCNFTMGHPWNLN
Sbjct: 661 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVDEIHNHTNSPCNFTMGHPWNLN 720

Query: 721 SPSITIAHLVGTQTVTRTVTNVAEEETYTITARMDPAVAIETSPPAMTLRSGSSRKFSVS 780
           +PSITIAHLVGT+TVTRTVTNVAEEETYTITARMDPA+AIETSPPAMTLRSGSSRKFSV+
Sbjct: 721 TPSITIAHLVGTKTVTRTVTNVAEEETYTITARMDPAIAIETSPPAMTLRSGSSRKFSVT 780

Query: 781 LTARSLTGTYSFGEVLLKGSRGHKVRIPVVAMGYQR 817
           LTARSLTGTYSFGEVLLKGSRGHKVRIPVVAMGYQR
Sbjct: 781 LTARSLTGTYSFGEVLLKGSRGHKVRIPVVAMGYQR 816

BLAST of Lag0006242 vs. ExPASy Swiss-Prot
Match: O64481 (Subtilisin-like protease SBT2.5 OS=Arabidopsis thaliana OX=3702 GN=SBT2.5 PE=2 SV=1)

HSP 1 Score: 1353.6 bits (3502), Expect = 0.0e+00
Identity = 664/809 (82.08%), Postives = 727/809 (89.86%), Query Frame = 0

Query: 9   VLVFFALLVVGKAEIYIVTIDGEPIVSYKGDLDGFEATALESDEKIDPTSEIVTSYARHL 68
           + V F LLV   AE+YIVT++G+PI+SYKG  +GFEATA+ESDEKID +SE+VT YARHL
Sbjct: 7   IFVVFVLLVAVTAEVYIVTMEGDPIISYKGGENGFEATAVESDEKIDTSSELVTVYARHL 66

Query: 69  ENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDISPEQAEILRHTPIVKSVERDWKVRKL 128
           E KHDM+LGMLFE GS+KKLYSYKHLINGFA  +SPEQAE LR  P V+SV++DWKVR+L
Sbjct: 67  ERKHDMILGMLFEEGSYKKLYSYKHLINGFAAHVSPEQAETLRRAPGVRSVDKDWKVRRL 126

Query: 129 TTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPFGPCSKYKG 188
           TTHTPEFLGLPT VWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFA+++  P+GP   YKG
Sbjct: 127 TTHTPEFLGLPTDVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFASHHRLPYGPLPHYKG 186

Query: 189 KCEVDPDTKKDFCNGKIIGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHTAAIAAGNN 248
           KCE DP TKK FCN KI+GAQHFAEAAKAAGAFNP I +ASP+DGDGHGSHTAAIAAGNN
Sbjct: 187 KCEEDPHTKKSFCNRKIVGAQHFAEAAKAAGAFNPDIDYASPMDGDGHGSHTAAIAAGNN 246

Query: 249 GIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGVDILSLSVG 308
           GIP+RMHGYEFGKASGMAPRARIAVYKALYR+FGGFVADVVAAIDQAVHDGVDILSLSVG
Sbjct: 247 GIPLRMHGYEFGKASGMAPRARIAVYKALYRLFGGFVADVVAAIDQAVHDGVDILSLSVG 306

Query: 309 PNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIATVAAAIDD 368
           PNSPP TTK T+LNPFDATLL AVKAGVFVAQAAGNGGPFPKTLVSYSPWI TVAAAIDD
Sbjct: 307 PNSPPTTTKTTFLNPFDATLLGAVKAGVFVAQAAGNGGPFPKTLVSYSPWITTVAAAIDD 366

Query: 369 RRYKNHLKLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVMKYSPSDCQRPEVLNK 428
           RRYKNHL LGNGK+LAG+GLSP T  +R YTLV+ANDVLLDSSV KY+PSDCQRPEV NK
Sbjct: 367 RRYKNHLTLGNGKMLAGMGLSPPTRPHRLYTLVSANDVLLDSSVSKYNPSDCQRPEVFNK 426

Query: 429 RLVEGKVLLCGYSFSFVVGTASIKKVSQTAKALGAAGFVLAVENISPGAKFDPVPVGIPG 488
           +LVEG +LLCGYSF+FVVGTASIKKV  TAK LGAAGFVL VEN+SPG KFDPVP  IPG
Sbjct: 427 KLVEGNILLCGYSFNFVVGTASIKKVVATAKHLGAAGFVLVVENVSPGTKFDPVPSAIPG 486

Query: 489 ILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEVALFSARGP 548
           ILITDVSKSMDLIDYYN ST RDWTGRVKSF A GSIGDGL P+L+KSAP+VALFSARGP
Sbjct: 487 ILITDVSKSMDLIDYYNASTSRDWTGRVKSFKAEGSIGDGLAPVLHKSAPQVALFSARGP 546

Query: 549 NIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMAAPHIAGIA 608
           N +DFSFQDADLLKPDILAPG LIWAAW PNGTDEPNYVGEGFA+ISGTSMAAPHIAGIA
Sbjct: 547 NTKDFSFQDADLLKPDILAPGYLIWAAWCPNGTDEPNYVGEGFALISGTSMAAPHIAGIA 606

Query: 609 ALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLVTATPFDYGSGHVN 668
           ALVKQKHP WSPAAIKSALMTTST +DRAGR L+AQQ+S+TEA+ LV ATPFDYGSGHVN
Sbjct: 607 ALVKQKHPQWSPAAIKSALMTTSTVIDRAGRLLQAQQYSDTEAVTLVKATPFDYGSGHVN 666

Query: 669 PRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTKTPCNFTMGHPWNLNSPSITIAH 728
           P AALDPGLIFDAGYEDYLGFLCTT GI+ HEI NYT T CN+ M HP N N+PSI ++H
Sbjct: 667 PSAALDPGLIFDAGYEDYLGFLCTTPGISAHEIRNYTNTACNYDMKHPSNFNAPSIAVSH 726

Query: 729 LVGTQTVTRTVTNVAE-EETYTITARMDPAVAIETSPPAMTLRSGSSRKFSVSLTARSLT 788
           LVGTQTVTR VTNVAE EETYTITARM P++AIE +PPAMTLR G++R FSV++T RS++
Sbjct: 727 LVGTQTVTRKVTNVAEVEETYTITARMQPSIAIEVNPPAMTLRPGATRTFSVTMTVRSVS 786

Query: 789 GTYSFGEVLLKGSRGHKVRIPVVAMGYQR 817
           G YSFGEV LKGSRGHKVRIPVVA+G++R
Sbjct: 787 GVYSFGEVKLKGSRGHKVRIPVVALGHRR 815

BLAST of Lag0006242 vs. ExPASy Swiss-Prot
Match: Q9SZV5 (Subtilisin-like protease SBT2.6 OS=Arabidopsis thaliana OX=3702 GN=SBT2.6 PE=2 SV=1)

HSP 1 Score: 1347.4 bits (3486), Expect = 0.0e+00
Identity = 664/810 (81.98%), Postives = 730/810 (90.12%), Query Frame = 0

Query: 9   VLVFFALLVVGKAEIYIVTIDGEPIVSYKGDLDGFEATALESDEKIDPTSEIVTSYARHL 68
           VLVFF   +   AEIYIVT++GEPI+SYKG  +GFEATA+ESDEKID TSE+VTSYARHL
Sbjct: 7   VLVFFTCFLTVTAEIYIVTMEGEPIISYKGGDNGFEATAVESDEKIDTTSELVTSYARHL 66

Query: 69  ENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDISPEQAEILRHTPIVKSVERDWKVRKL 128
           E KHDMLLGMLF  GS+KKLYSYKHLINGFA  +SP+QAE+LR  P VKSV+RDWKVRKL
Sbjct: 67  ERKHDMLLGMLFVEGSYKKLYSYKHLINGFAAHVSPDQAEMLRRAPGVKSVDRDWKVRKL 126

Query: 129 TTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTE-PFGPCSKYK 188
           TTHTP+FLGLPT VWPTGGG+DRAGEDIVIGF+DSGI+PHHPSFA+++T  P+GP   YK
Sbjct: 127 TTHTPQFLGLPTDVWPTGGGYDRAGEDIVIGFIDSGIFPHHPSFASHHTTVPYGPHPSYK 186

Query: 189 GKCEVDPDTKKDFCNGKIIGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHTAAIAAGN 248
           GKCE DP TK  FCNGKIIGAQHFAEAAKAAGAFNP I FASP+DGDGHGSHTAAIAAGN
Sbjct: 187 GKCEEDPHTKISFCNGKIIGAQHFAEAAKAAGAFNPDIDFASPMDGDGHGSHTAAIAAGN 246

Query: 249 NGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGVDILSLSV 308
           NGIPVRMHGYEFGKASGMAPRARIAVYKALYR+FGGFVADVVAAIDQAVHDGVDILSLSV
Sbjct: 247 NGIPVRMHGYEFGKASGMAPRARIAVYKALYRLFGGFVADVVAAIDQAVHDGVDILSLSV 306

Query: 309 GPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIATVAAAID 368
           GPNSPPATTK T+LNPFDATLL AVKAGVFVAQAAGNGGPFPKTLVSYSPWI TVAAAID
Sbjct: 307 GPNSPPATTKTTFLNPFDATLLGAVKAGVFVAQAAGNGGPFPKTLVSYSPWITTVAAAID 366

Query: 369 DRRYKNHLKLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVMKYSPSDCQRPEVLN 428
           DRRYKNHL LGNGK+LAG+GLSP+T  +R+Y +V+ANDVLL SS MKY+PSDCQ+PEVLN
Sbjct: 367 DRRYKNHLTLGNGKMLAGIGLSPSTRPHRSYKMVSANDVLLGSSGMKYNPSDCQKPEVLN 426

Query: 429 KRLVEGKVLLCGYSFSFVVGTASIKKVSQTAKALGAAGFVLAVENISPGAKFDPVPVGIP 488
           K+LVEG +LLCGYSF+FV G+ASIKKV++TAK LGAAGFVL VEN+SPG KFDPVP  IP
Sbjct: 427 KKLVEGNILLCGYSFNFVAGSASIKKVAETAKHLGAAGFVLVVENVSPGTKFDPVPSCIP 486

Query: 489 GILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEVALFSARG 548
           GILITDVSKSMDLIDYYN +T RDW GRVK F A GSIGDGL P+L+KSAPEVALFSARG
Sbjct: 487 GILITDVSKSMDLIDYYNVTTSRDWMGRVKDFKAEGSIGDGLEPILHKSAPEVALFSARG 546

Query: 549 PNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMAAPHIAGI 608
           PN +DFSFQDADLLKPDILAPGSLIW+AWS NGTDE NY+GEGFA+ISGTSMAAPHIAGI
Sbjct: 547 PNTKDFSFQDADLLKPDILAPGSLIWSAWSANGTDEANYIGEGFALISGTSMAAPHIAGI 606

Query: 609 AALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLVTATPFDYGSGHV 668
           AALVKQKHP WSPAAIKSALMTTST +DRAGRPL+AQQ+SETE + LV ATPFDYGSGHV
Sbjct: 607 AALVKQKHPQWSPAAIKSALMTTSTVIDRAGRPLQAQQYSETETVTLVKATPFDYGSGHV 666

Query: 669 NPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTKTPCNFTMGHPWNLNSPSITIA 728
           NP AALDPGLIFDAGYEDY+GFLCTT GI+ HEI N+T TPCNF M HP N N+PSI I+
Sbjct: 667 NPSAALDPGLIFDAGYEDYIGFLCTTPGIDAHEIKNFTNTPCNFKMVHPSNFNTPSIAIS 726

Query: 729 HLVGTQTVTRTVTNVA-EEETYTITARMDPAVAIETSPPAMTLRSGSSRKFSVSLTARSL 788
           HLV TQTVTR VTNVA EEETYTIT+RM+PA+AIE SPPAMT+R+G+SR FSV+LT RS+
Sbjct: 727 HLVRTQTVTRRVTNVAEEEETYTITSRMEPAIAIEVSPPAMTVRAGASRTFSVTLTVRSV 786

Query: 789 TGTYSFGEVLLKGSRGHKVRIPVVAMGYQR 817
           TG YSFG+V LKGSRGHKV +PVVAMG +R
Sbjct: 787 TGAYSFGQVTLKGSRGHKVTLPVVAMGQRR 816

BLAST of Lag0006242 vs. ExPASy Swiss-Prot
Match: Q9FI12 (Subtilisin-like protease SBT2.3 OS=Arabidopsis thaliana OX=3702 GN=SBT2.3 PE=2 SV=1)

HSP 1 Score: 661.0 bits (1704), Expect = 1.8e-188
Identity = 371/810 (45.80%), Postives = 502/810 (61.98%), Query Frame = 0

Query: 23  IYIVTIDGEPIVSYKGDLDGFEATALESDE-----KIDPTSEIVTSYARHLENK------ 82
           +YIVT+   PIV        FE   L+  +     K+ P +    S  RH ++K      
Sbjct: 38  VYIVTLKQPPIVHL------FEEQELKHKKSKFTPKLRPRN---NSRKRHGKSKIPSVVQ 97

Query: 83  -HDMLLGMLFERGSFKKLYSYKHLINGFAVDISPEQAEILRHTPIVKSVERDWKVRKLTT 142
            HD  L    +   + KLYSY +LINGFA+ I+ +QAE L     V ++  D+ VR  TT
Sbjct: 98  SHDSFLRKTLKGEKYIKLYSYHYLINGFALFINSQQAEKLSMRKEVANIVLDYSVRTATT 157

Query: 143 HTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPFGPCSK-YKGK 202
           +TP+F+GLP G W   GGF+ AGE ++IGF+D+GI P+HPSF   +++   P  K + G 
Sbjct: 158 YTPQFMGLPQGAWVKEGGFEIAGEGVIIGFIDTGIDPNHPSFNDNDSKRSYPIPKHFSGV 217

Query: 203 CEVDPDTKKDFCNGKIIGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHTAAIAAGNNG 262
           CEV PD     CN K+IGA+HFA++A   G FN +  +ASP DGDGHG+HTA++AAGN+G
Sbjct: 218 CEVTPDFPSGSCNKKLIGARHFAQSAVTRGIFNSSEDYASPFDGDGHGTHTASVAAGNHG 277

Query: 263 IPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGVDILSLSVGP 322
           +PV +  + FG ASG+APRA I+VYKALY+ FGGF ADVVAAIDQA  DGVDILSLS+ P
Sbjct: 278 VPVIVSNHNFGYASGIAPRAFISVYKALYKSFGGFAADVVAAIDQAAQDGVDILSLSITP 337

Query: 323 NSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIATVAAAIDDR 382
           N  P     T+ NP D  LLSAVKAG+FV QAAGN GP PKT+ S+SPWI TV A+  DR
Sbjct: 338 NRKPPGV-ATFFNPIDMALLSAVKAGIFVVQAAGNTGPAPKTMSSFSPWIFTVGASSHDR 397

Query: 383 RYKNHLKLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVMKYS--PSDCQRPEVLN 442
            Y N L LGN   + G+G +  T   + Y +++A   L +S+ +       +CQ  E  +
Sbjct: 398 VYSNSLTLGNNVTIPGMGFAIPTDSGKMYKMISAFHALNNSTSVDKDMYVGECQDYENFD 457

Query: 443 KRLVEGKVLLCGYSFSFVVGTASIKKVSQTAKALGAAGFVLAVENISPGAKFDPVPVGIP 502
           +  V GK+L+C YS  FV+G ++IK+    AK L A G +  ++    G + +P P+ +P
Sbjct: 458 QDRVSGKLLICSYSARFVLGLSTIKQALDVAKNLSATGVIFYIDPYVLGFEINPTPMDMP 517

Query: 503 GILITDVSKSMDLIDYYNTSTPRDWTGR-VKSFDAVGSIGDGLMPLLYKSAPEVALFSAR 562
           GI+I  V  S  L+ YYN+S  RD T + + SF AV +I  GL       AP+V  +SAR
Sbjct: 518 GIIIPSVEDSKTLLKYYNSSIQRDVTTKEIVSFGAVAAIEGGLNANFSNRAPKVMYYSAR 577

Query: 563 GPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMAAPHIAG 622
           GP+  D SF DAD+LKP+++APG+ IW AWS   TD   + GE FAM+SGTSMAAPH+AG
Sbjct: 578 GPDPEDNSFNDADVLKPNLVAPGNSIWGAWSSASTDSTEFEGEKFAMMSGTSMAAPHVAG 637

Query: 623 IAALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLVTATPFDYGSGH 682
           +AAL+KQ +P ++P+ I SAL TT+   D  G P+ AQ+        L TATP D GSG 
Sbjct: 638 VAALIKQSYPQFTPSTISSALSTTALLNDNKGSPIMAQRTYSNPDQSLYTATPSDMGSGF 697

Query: 683 VNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHE--IHNYT--KTPCNFTMGHPWNLNSP 742
           VN  AALDPGL+FD  +EDY+ FLC   GIN  +  + NYT  + P N T    ++LN P
Sbjct: 698 VNATAALDPGLVFDTSFEDYISFLC---GINGSDTVVFNYTGFRCPANNTPVSGFDLNLP 757

Query: 743 SITIAHLVGTQTVTRTVTNVAEEETYTITARMDPAVAIETSPPAMTLRSGSSRKFSVSLT 802
           SIT++ L GTQT  R++ N+A  ETY +       V+++ SP   ++  G ++  SV+LT
Sbjct: 758 SITVSTLSGTQTFQRSMRNIAGNETYNVGWSPPYGVSMKVSPTQFSIAMGENQVLSVTLT 817

Query: 803 ARSLTGTYSFGEVLLKGSRGHKVRIPVVAM 813
               + + SFG + L G+ GH V IPV  +
Sbjct: 818 VTKNSSSSSFGRIGLFGNTGHIVNIPVTVI 834

BLAST of Lag0006242 vs. ExPASy Swiss-Prot
Match: Q9SUN6 (Subtilisin-like protease SBT2.2 OS=Arabidopsis thaliana OX=3702 GN=SBT2.2 PE=3 SV=1)

HSP 1 Score: 649.4 bits (1674), Expect = 5.3e-185
Identity = 355/747 (47.52%), Postives = 472/747 (63.19%), Query Frame = 0

Query: 72  HDMLLGMLFERGSFKKLYSYKHLINGFAVDISPEQAEILRHTPIVKSVERDWKVRKLTTH 131
           HD LL    +   + KLYS+ +LINGFAV +S +QAE L     V ++  D+ VR  TT+
Sbjct: 103 HDSLLRNALKGEKYIKLYSFHYLINGFAVFVSSQQAETLSRRREVANIVLDFSVRTATTY 162

Query: 132 TPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNT--EPFGPCSKYKGK 191
           TP+F+GLP G W   GG++ AGE IVIGF+D+GI P HPSF   +T    +   + + G 
Sbjct: 163 TPQFMGLPKGAWVKEGGYETAGEGIVIGFIDTGIDPTHPSFNGTDTSQRQYPIPNHFSGV 222

Query: 192 CEVDPDTKKDFCNGKIIGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHTAAIAAGNNG 251
           CEV PD     CN K++GA+HFA++A   G FN +  +ASP DGDGHG+HTA+IAAGN+G
Sbjct: 223 CEVTPDFPSGSCNRKLVGARHFAQSAITRGIFNSSEDYASPFDGDGHGTHTASIAAGNHG 282

Query: 252 IPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGVDILSLSVGP 311
           +   + G+ FG ASG+APRA I+VYKALY+ FGGF ADVVAAIDQA  DGVDILSLS+ P
Sbjct: 283 VSAVVSGHNFGSASGIAPRAHISVYKALYKSFGGFAADVVAAIDQAAQDGVDILSLSITP 342

Query: 312 NSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIATVAAAIDDR 371
           N  P     T+ NP D  +LSAVKAG+FV QAAGN GP PK++ S+SPWI TV AA  DR
Sbjct: 343 NRRPPGV-ATFFNPLDMAMLSAVKAGIFVVQAAGNTGPSPKSMSSFSPWIFTVGAASHDR 402

Query: 372 RYKNHLKLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVM---KYSPSDCQRPEVL 431
            Y N + LGN   + G+GL+  T   + YT+++A D L + S +        +CQ     
Sbjct: 403 DYSNSIVLGNNVSIPGVGLALRTDEGKKYTMISALDALKNKSSVVDKDMYVGECQDYGSF 462

Query: 432 NKRLVEGKVLLCGYSFSFVVGTASIKKVSQTAKALGAAGFVLAVENISPGAKFDPVPVGI 491
           +K ++ G +L+C YS  FV+G ++IK+    AK L A G V  ++    G + +P P+ +
Sbjct: 463 DKDVIRGNLLICSYSIRFVLGLSTIKQALAVAKNLSAKGVVFYMDPYVLGFQINPTPMDM 522

Query: 492 PGILITDVSKSMDLIDYYNTSTPRDWTGR-VKSFDAVGSIGDGLMPLLYKSAPEVALFSA 551
           PGI+I     S  L+ YYN+S  RD T + +  F AV +I  G        AP++  +SA
Sbjct: 523 PGIIIPSAEDSKVLLKYYNSSLVRDGTTKEIVRFGAVAAIAGGQNANFSNRAPKIMYYSA 582

Query: 552 RGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMAAPHIA 611
           RGP+ +D  F DAD+LKP+++APG+ IW AWS   T+   + GE FAM+SGTSMAAPH+A
Sbjct: 583 RGPDPQDSLFNDADILKPNLVAPGNSIWGAWSSAATESTEFEGESFAMMSGTSMAAPHVA 642

Query: 612 GIAALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLVTATPFDYGSG 671
           G+AALVKQK   +SP+AI SAL TTS   D  G  + AQ+        +  ATPFD G+G
Sbjct: 643 GVAALVKQKFRKFSPSAIASALSTTSVLFDNKGEAIMAQRAYANPDQTISPATPFDMGNG 702

Query: 672 HVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTKTPC---NFTMGHPWNLNSP 731
            VN  AALDPGLIFD  +EDY+ FLC   G +   + NYT T C   N T+    +LN P
Sbjct: 703 FVNATAALDPGLIFDTSFEDYMSFLCGING-SAPVVFNYTGTNCLRNNATISGS-DLNLP 762

Query: 732 SITIAHLVGTQTVTRTVTNVAEEETYTITARMDPAVAIETSPPAMTLRSGSSRKFSVSLT 791
           SIT++ L  T+TV R +TN+A  ETYT++      V I  SP   ++ SG ++  SV LT
Sbjct: 763 SITVSKLNNTRTVQRLMTNIAGNETYTVSLITPFDVLINVSPTQFSIASGETKLLSVILT 822

Query: 792 ARSLTGTYSFGEVLLKGSRGHKVRIPV 810
           A+  +   SFG + L G+ GH VRIPV
Sbjct: 823 AKRNSSISSFGGIKLLGNAGHIVRIPV 846

BLAST of Lag0006242 vs. ExPASy Swiss-Prot
Match: Q9SA75 (Subtilisin-like protease SBT2.1 OS=Arabidopsis thaliana OX=3702 GN=SBT2.1 PE=2 SV=1)

HSP 1 Score: 633.6 bits (1633), Expect = 3.0e-180
Identity = 363/810 (44.81%), Postives = 497/810 (61.36%), Query Frame = 0

Query: 23  IYIVTIDGEPIVSYKGDLDGFEATALESDEKIDPTSEIV---TSYARHLENKHDMLLGML 82
           +YIVT+   P V + G        + +S   +  TS  +    + +  +   HD LL  +
Sbjct: 36  VYIVTLKDRPSVHFSG------RESSDSKHSLTATSSQIYRTLNRSASIIRVHDSLLRNV 95

Query: 83  FERGSFKKLYSYKHLINGFAVDISPEQAEILRHTPIVKSVERDWKVRKLTTHTPEFLGLP 142
             + ++ KLYSY +LINGF+  ++ +QA+ L     V++V  D+ V K TTHTP+FLGLP
Sbjct: 96  LRKENYLKLYSYHYLINGFSAVLTRKQADRLAAREEVENVVLDFLVEKATTHTPQFLGLP 155

Query: 143 TGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFA------TYNTEPFGPCSKYKGKCEVD 202
            G W   GG + AGE +VIGF+D+GI P HPSF+      TY+  P      + G CEV 
Sbjct: 156 RGAWLRDGGSEYAGEGVVIGFIDTGIDPTHPSFSDKISGHTYSVPP-----HFTGVCEVT 215

Query: 203 PDTKKDFCNGKIIGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHTAAIAAGNNGIPVR 262
                  CN K+IGA+HFAE+A + G  N +   ASP DG+GHG+HTA++AAGN+GIPV 
Sbjct: 216 IGFPPGSCNRKLIGARHFAESALSRGVLNSSQDDASPFDGEGHGTHTASVAAGNHGIPVV 275

Query: 263 MHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGVDILSLSVGPNSPP 322
           + G+  G ASGMAPRA IA+YKALY+ FGGF AD++AAIDQA  DGVDI++LS+ PN  P
Sbjct: 276 VAGHRLGNASGMAPRAHIAIYKALYKRFGGFAADIIAAIDQAAQDGVDIINLSITPNRRP 335

Query: 323 ATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIATVAAAIDDRRYKN 382
                T+ NP D  LLSAVKAG+FV QAAGN GP PK++ S+SPWI TV A   DR Y N
Sbjct: 336 PGI-ATFFNPIDMALLSAVKAGIFVVQAAGNTGPAPKSMSSFSPWIFTVGATSHDRVYSN 395

Query: 383 HLKLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVMKYS--PSDCQRPEVLNKRLV 442
            + LGN   + G+GL+  T +   + LV A   L + + +  +    +CQ     +++LV
Sbjct: 396 SIILGNNVTIPGVGLASGTRI--MHKLVLATHALRNGTTVMDAIYVGECQDSSSFDQKLV 455

Query: 443 EGKVLLCGYSFSFVVGTASIKKVSQTAKALGAAGFVLAVENISPGAKFDPVPVGIPGILI 502
           +GK+L+C Y+  F++G ++IK+   TAK L AAG V  ++  + G +    P+ IPGILI
Sbjct: 456 QGKILVCSYTVRFILGVSTIKQALLTAKNLTAAGLVFYIDPSATGFQMTSSPMDIPGILI 515

Query: 503 TDVSKSMDLIDYYNTSTPRD-WTGRVKSFDAVGSIGDGLMPLLYKSAPEVALFSARGPNI 562
           +    S  L+ YYN+S  R+  +G++    +V  I  G+ P    +AP+V  FSARGP+ 
Sbjct: 516 SSPQDSQALLRYYNSSLLRENGSGKIVGSASVAKIVGGMRPTYGITAPKVMYFSARGPDP 575

Query: 563 RDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMAAPHIAGIAAL 622
            D SF DAD++KP+++APG+ IW AWSP G    ++ GE FAM SGTSM+APH+ GIAAL
Sbjct: 576 EDDSFVDADIMKPNLVAPGNAIWGAWSPLGIGTNDFQGERFAMESGTSMSAPHVTGIAAL 635

Query: 623 VKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLVTATPFDYGSGHVNPR 682
           +KQK P+++PAAI SAL TT++  DR G  + AQ+      +    ATPFD GSG VN  
Sbjct: 636 IKQKFPHFTPAAIASALSTTASLSDRKGEHIMAQRTVLNPDISQSPATPFDMGSGFVNAT 695

Query: 683 AALDPGLIFDAGYEDYLGFLCTTAGINVHE--IHNYTKTPC---NFTMGHPWNLNSPSIT 742
           AALDPGLIFD GY +Y+ FLC   GIN     + NYT   C   N ++    +LN PS+T
Sbjct: 696 AALDPGLIFDIGYNEYMKFLC---GINGSSPVVLNYTGESCSSYNSSLAAS-DLNLPSVT 755

Query: 743 IAHLVGTQTVTRTVTNV---AEEETYTITARMDPAVAIETSPPAMTLRSGSSRKFSVSLT 802
           IA LVGT+ V R VTN+   A  ETY +      +V+++ SP   T+ +G +R  S+   
Sbjct: 756 IAKLVGTRAVLRWVTNIATTATNETYIVGWMAPDSVSVKVSPAKFTIGNGQTRVLSLVFR 815

Query: 803 ARSLTGTYSFGEVLLKGSRGHKVRIPVVAM 813
           A       SFG + L G RGH V IPV  +
Sbjct: 816 AMKNVSMASFGRIGLFGDRGHVVNIPVAVI 827

BLAST of Lag0006242 vs. ExPASy TrEMBL
Match: A0A6J1KAV8 (subtilisin-like protease SBT2.5 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111491718 PE=3 SV=1)

HSP 1 Score: 1589.7 bits (4115), Expect = 0.0e+00
Identity = 788/816 (96.57%), Postives = 805/816 (98.65%), Query Frame = 0

Query: 1   MGVNFQRTVLVFFALLVVGKAEIYIVTIDGEPIVSYKGDLDGFEATALESDEKIDPTSEI 60
           M VNFQ TVLVF  LLVVGKAEIYIVTI+GEPIVSYKGDLDGFEATA+ES+EKIDPTSEI
Sbjct: 1   MVVNFQCTVLVFLVLLVVGKAEIYIVTIEGEPIVSYKGDLDGFEATAMESNEKIDPTSEI 60

Query: 61  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDISPEQAEILRHTPIVKSVE 120
           VTSYARHLE+KHDMLLGMLFERGSFKKLYSYKHLINGFAVDI+PEQAE LRHTPIVKSVE
Sbjct: 61  VTSYARHLESKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITPEQAETLRHTPIVKSVE 120

Query: 121 RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180
            DWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF
Sbjct: 121 MDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180

Query: 181 GPCSKYKGKCEVDPDTKKDFCNGKIIGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHT 240
           GPC KYKGKCEVDPDTKK+FCNGKI+GAQHFAEAAKAAGAFNPAIHF SPLDGDGHGSHT
Sbjct: 181 GPCLKYKGKCEVDPDTKKEFCNGKIVGAQHFAEAAKAAGAFNPAIHFESPLDGDGHGSHT 240

Query: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300
           AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV
Sbjct: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300

Query: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360
           DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA
Sbjct: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360

Query: 361 TVAAAIDDRRYKNHLKLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVMKYSPSDC 420
           TVAAAIDDRRYKNHL LGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSV KYSPSDC
Sbjct: 361 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVSKYSPSDC 420

Query: 421 QRPEVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTAKALGAAGFVLAVENISPGAKFD 480
           QRP+VLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTA+ALGAAGFVLAVENISPG+KFD
Sbjct: 421 QRPDVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTARALGAAGFVLAVENISPGSKFD 480

Query: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 540
           PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV
Sbjct: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 540

Query: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600
           ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA
Sbjct: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600

Query: 601 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLVTATPF 660
           APHIAGI+ALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLV+ATPF
Sbjct: 601 APHIAGISALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLVSATPF 660

Query: 661 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTKTPCNFTMGHPWNLN 720
           DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYT +PCNFTMGHPWNLN
Sbjct: 661 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPCNFTMGHPWNLN 720

Query: 721 SPSITIAHLVGTQTVTRTVTNVAEEETYTITARMDPAVAIETSPPAMTLRSGSSRKFSVS 780
           +PSITIAHLVGT+TVTRTVTNVAEEETYTITARMDPAVAIETSPPAMTLRSGSSRKFSV+
Sbjct: 721 TPSITIAHLVGTKTVTRTVTNVAEEETYTITARMDPAVAIETSPPAMTLRSGSSRKFSVT 780

Query: 781 LTARSLTGTYSFGEVLLKGSRGHKVRIPVVAMGYQR 817
           LT+RSLTGTYSFGEVLLKGSRGHKVRIPVVAMGYQR
Sbjct: 781 LTSRSLTGTYSFGEVLLKGSRGHKVRIPVVAMGYQR 816

BLAST of Lag0006242 vs. ExPASy TrEMBL
Match: A0A6J1EPH6 (subtilisin-like protease SBT2.5 OS=Cucurbita moschata OX=3662 GN=LOC111436195 PE=3 SV=1)

HSP 1 Score: 1588.2 bits (4111), Expect = 0.0e+00
Identity = 786/816 (96.32%), Postives = 805/816 (98.65%), Query Frame = 0

Query: 1   MGVNFQRTVLVFFALLVVGKAEIYIVTIDGEPIVSYKGDLDGFEATALESDEKIDPTSEI 60
           M VNFQ TVLVF  L+VVGKAEIYIVTI+GEPIVSYKGDLDGFEATA+ES+EKIDPTSEI
Sbjct: 1   MVVNFQCTVLVFLVLVVVGKAEIYIVTIEGEPIVSYKGDLDGFEATAMESNEKIDPTSEI 60

Query: 61  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDISPEQAEILRHTPIVKSVE 120
           VTSYARHLE+KHDMLLGMLFERGSFKKLYSYKHLINGFAVDI+PEQAE LRHTPIVKSVE
Sbjct: 61  VTSYARHLESKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITPEQAETLRHTPIVKSVE 120

Query: 121 RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180
           RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF
Sbjct: 121 RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180

Query: 181 GPCSKYKGKCEVDPDTKKDFCNGKIIGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHT 240
           GPC KYKGKCEVDPDTKK+FCNGKI+GAQHFAEAAKAAGAFNPAIHF SPLDGDGHGSHT
Sbjct: 181 GPCLKYKGKCEVDPDTKKEFCNGKIVGAQHFAEAAKAAGAFNPAIHFESPLDGDGHGSHT 240

Query: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300
           AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV
Sbjct: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300

Query: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360
           DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA
Sbjct: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360

Query: 361 TVAAAIDDRRYKNHLKLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVMKYSPSDC 420
           TVAAAIDDRRYKNHL LGNGKILAGLGLSPATH NRTYTLVAANDVLLDSSV KYSPSDC
Sbjct: 361 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHFNRTYTLVAANDVLLDSSVSKYSPSDC 420

Query: 421 QRPEVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTAKALGAAGFVLAVENISPGAKFD 480
           QRP+VLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTA+ALGAAGFVLAVENISPG+KFD
Sbjct: 421 QRPDVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTARALGAAGFVLAVENISPGSKFD 480

Query: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 540
           PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV
Sbjct: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 540

Query: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600
           ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA
Sbjct: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600

Query: 601 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLVTATPF 660
           APHIAGI+ALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLV+ATPF
Sbjct: 601 APHIAGISALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLVSATPF 660

Query: 661 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTKTPCNFTMGHPWNLN 720
           DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHN+T +PCNFTMGHPWNLN
Sbjct: 661 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNHTNSPCNFTMGHPWNLN 720

Query: 721 SPSITIAHLVGTQTVTRTVTNVAEEETYTITARMDPAVAIETSPPAMTLRSGSSRKFSVS 780
           +PSITIAHLVGT+TVTRTVTNVAEEETYTITARMDPA+AIETSPPAMTLRSGSSRKFSV+
Sbjct: 721 TPSITIAHLVGTKTVTRTVTNVAEEETYTITARMDPAIAIETSPPAMTLRSGSSRKFSVT 780

Query: 781 LTARSLTGTYSFGEVLLKGSRGHKVRIPVVAMGYQR 817
           LTARSLTGTYSFGEVLLKGSRGHKVRIPVVAMGYQR
Sbjct: 781 LTARSLTGTYSFGEVLLKGSRGHKVRIPVVAMGYQR 816

BLAST of Lag0006242 vs. ExPASy TrEMBL
Match: A0A5D3CEZ7 (Subtilisin-like protease SBT2.5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1411G00120 PE=3 SV=1)

HSP 1 Score: 1584.3 bits (4101), Expect = 0.0e+00
Identity = 787/816 (96.45%), Postives = 800/816 (98.04%), Query Frame = 0

Query: 1   MGVNFQRTVLVFFALLVVGKAEIYIVTIDGEPIVSYKGDLDGFEATALESDEKIDPTSEI 60
           M VNFQ TVLVF A+L VGKAEIYIVTIDGEPIVSYKGDLDGFEATA+ESDEKIDPTSEI
Sbjct: 1   MVVNFQYTVLVFLAILFVGKAEIYIVTIDGEPIVSYKGDLDGFEATAMESDEKIDPTSEI 60

Query: 61  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDISPEQAEILRHTPIVKSVE 120
           VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDI+ EQAE LR TPIVKSVE
Sbjct: 61  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITHEQAETLRRTPIVKSVE 120

Query: 121 RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180
           RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF
Sbjct: 121 RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180

Query: 181 GPCSKYKGKCEVDPDTKKDFCNGKIIGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHT 240
           GPC KYKGKCEVDP+TKKDFCNGKI+GA+HFAEAAKAAGAFNP IHFASPLDGDGHGSHT
Sbjct: 181 GPCMKYKGKCEVDPNTKKDFCNGKIVGARHFAEAAKAAGAFNPDIHFASPLDGDGHGSHT 240

Query: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300
           AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV
Sbjct: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300

Query: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360
           DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA
Sbjct: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360

Query: 361 TVAAAIDDRRYKNHLKLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVMKYSPSDC 420
           TVAAAIDDRRYKNHL LGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSV KYSPSDC
Sbjct: 361 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVTKYSPSDC 420

Query: 421 QRPEVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTAKALGAAGFVLAVENISPGAKFD 480
           Q+PE+LNK LVEGKVLLCGYSFSFVVGTASIKKVSQTAKALGAAGFVLAVENISPGAKFD
Sbjct: 421 QKPELLNKHLVEGKVLLCGYSFSFVVGTASIKKVSQTAKALGAAGFVLAVENISPGAKFD 480

Query: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 540
           PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV
Sbjct: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 540

Query: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600
           ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA
Sbjct: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600

Query: 601 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLVTATPF 660
           APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDR GRPLKAQQFSETEAMKLVTATPF
Sbjct: 601 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLVTATPF 660

Query: 661 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTKTPCNFTMGHPWNLN 720
           DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYT +PCNFTMGHPWNLN
Sbjct: 661 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPCNFTMGHPWNLN 720

Query: 721 SPSITIAHLVGTQTVTRTVTNVAEEETYTITARMDPAVAIETSPPAMTLRSGSSRKFSVS 780
           SPSITIAHLVGT+ VTRTVTNVAEEETYTITARMDPAVAIE +PPAMTLRSGSSRKFSV+
Sbjct: 721 SPSITIAHLVGTKIVTRTVTNVAEEETYTITARMDPAVAIEVNPPAMTLRSGSSRKFSVT 780

Query: 781 LTARSLTGTYSFGEVLLKGSRGHKVRIPVVAMGYQR 817
           LTARSLTGTYSFG+VLLKGSRGHKVRIPVVAMGYQR
Sbjct: 781 LTARSLTGTYSFGQVLLKGSRGHKVRIPVVAMGYQR 816

BLAST of Lag0006242 vs. ExPASy TrEMBL
Match: A0A1S3BRZ6 (subtilisin-like protease SBT2.5 OS=Cucumis melo OX=3656 GN=LOC103492606 PE=3 SV=1)

HSP 1 Score: 1583.9 bits (4100), Expect = 0.0e+00
Identity = 787/816 (96.45%), Postives = 800/816 (98.04%), Query Frame = 0

Query: 1   MGVNFQRTVLVFFALLVVGKAEIYIVTIDGEPIVSYKGDLDGFEATALESDEKIDPTSEI 60
           M VNFQ TVLVF A+L VGKAEIYIVTIDGEPIVSYKGDLDGFEATA+ESDEKIDPTSEI
Sbjct: 1   MVVNFQYTVLVFLAILFVGKAEIYIVTIDGEPIVSYKGDLDGFEATAMESDEKIDPTSEI 60

Query: 61  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDISPEQAEILRHTPIVKSVE 120
           VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDI+ EQAE LR TPIVKSVE
Sbjct: 61  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITHEQAESLRRTPIVKSVE 120

Query: 121 RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180
           RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF
Sbjct: 121 RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180

Query: 181 GPCSKYKGKCEVDPDTKKDFCNGKIIGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHT 240
           GPC KYKGKCEVDP+TKKDFCNGKI+GA+HFAEAAKAAGAFNP IHFASPLDGDGHGSHT
Sbjct: 181 GPCMKYKGKCEVDPNTKKDFCNGKIVGARHFAEAAKAAGAFNPDIHFASPLDGDGHGSHT 240

Query: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300
           AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV
Sbjct: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300

Query: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360
           DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA
Sbjct: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360

Query: 361 TVAAAIDDRRYKNHLKLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVMKYSPSDC 420
           TVAAAIDDRRYKNHL LGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSV KYSPSDC
Sbjct: 361 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVTKYSPSDC 420

Query: 421 QRPEVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTAKALGAAGFVLAVENISPGAKFD 480
           Q+PE+LNK LVEGKVLLCGYSFSFVVGTASIKKVSQTAKALGAAGFVLAVENISPGAKFD
Sbjct: 421 QKPELLNKHLVEGKVLLCGYSFSFVVGTASIKKVSQTAKALGAAGFVLAVENISPGAKFD 480

Query: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 540
           PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV
Sbjct: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 540

Query: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600
           ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA
Sbjct: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600

Query: 601 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLVTATPF 660
           APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDR GRPLKAQQFSETEAMKLVTATPF
Sbjct: 601 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLVTATPF 660

Query: 661 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTKTPCNFTMGHPWNLN 720
           DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYT +PCNFTMGHPWNLN
Sbjct: 661 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPCNFTMGHPWNLN 720

Query: 721 SPSITIAHLVGTQTVTRTVTNVAEEETYTITARMDPAVAIETSPPAMTLRSGSSRKFSVS 780
           SPSITIAHLVGT+ VTRTVTNVAEEETYTITARMDPAVAIE +PPAMTLRSGSSRKFSV+
Sbjct: 721 SPSITIAHLVGTKIVTRTVTNVAEEETYTITARMDPAVAIEVNPPAMTLRSGSSRKFSVT 780

Query: 781 LTARSLTGTYSFGEVLLKGSRGHKVRIPVVAMGYQR 817
           LTARSLTGTYSFG+VLLKGSRGHKVRIPVVAMGYQR
Sbjct: 781 LTARSLTGTYSFGQVLLKGSRGHKVRIPVVAMGYQR 816

BLAST of Lag0006242 vs. ExPASy TrEMBL
Match: A0A5A7SQC1 (Subtilisin-like protease SBT2.5 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold506G00020 PE=3 SV=1)

HSP 1 Score: 1583.9 bits (4100), Expect = 0.0e+00
Identity = 787/816 (96.45%), Postives = 800/816 (98.04%), Query Frame = 0

Query: 1   MGVNFQRTVLVFFALLVVGKAEIYIVTIDGEPIVSYKGDLDGFEATALESDEKIDPTSEI 60
           M VNFQ TVLVF A+L VGKAEIYIVTIDGEPIVSYKGDLDGFEATA+ESDEKIDPTSEI
Sbjct: 1   MVVNFQYTVLVFLAILFVGKAEIYIVTIDGEPIVSYKGDLDGFEATAMESDEKIDPTSEI 60

Query: 61  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDISPEQAEILRHTPIVKSVE 120
           VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDI+ EQAE LR TPIVKSVE
Sbjct: 61  VTSYARHLENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDITHEQAESLRRTPIVKSVE 120

Query: 121 RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180
           RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF
Sbjct: 121 RDWKVRKLTTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPF 180

Query: 181 GPCSKYKGKCEVDPDTKKDFCNGKIIGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHT 240
           GPC KYKGKCEVDP+TKKDFCNGKI+GA+HFAEAAKAAGAFNP IHFASPLDGDGHGSHT
Sbjct: 181 GPCMKYKGKCEVDPNTKKDFCNGKIVGARHFAEAAKAAGAFNPDIHFASPLDGDGHGSHT 240

Query: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300
           AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV
Sbjct: 241 AAIAAGNNGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGV 300

Query: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360
           DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA
Sbjct: 301 DILSLSVGPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIA 360

Query: 361 TVAAAIDDRRYKNHLKLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVMKYSPSDC 420
           TVAAAIDDRRYKNHL LGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSV KYSPSDC
Sbjct: 361 TVAAAIDDRRYKNHLTLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVTKYSPSDC 420

Query: 421 QRPEVLNKRLVEGKVLLCGYSFSFVVGTASIKKVSQTAKALGAAGFVLAVENISPGAKFD 480
           Q+PE+LNK LVEGKVLLCGYSFSFVVGTASIKKVSQTAKALGAAGFVLAVENISPGAKFD
Sbjct: 421 QKPELLNKHLVEGKVLLCGYSFSFVVGTASIKKVSQTAKALGAAGFVLAVENISPGAKFD 480

Query: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 540
           PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV
Sbjct: 481 PVPVGIPGILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEV 540

Query: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600
           ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA
Sbjct: 541 ALFSARGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMA 600

Query: 601 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLVTATPF 660
           APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDR GRPLKAQQFSETEAMKLVTATPF
Sbjct: 601 APHIAGIAALVKQKHPNWSPAAIKSALMTTSTTMDRGGRPLKAQQFSETEAMKLVTATPF 660

Query: 661 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTKTPCNFTMGHPWNLN 720
           DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYT +PCNFTMGHPWNLN
Sbjct: 661 DYGSGHVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTNSPCNFTMGHPWNLN 720

Query: 721 SPSITIAHLVGTQTVTRTVTNVAEEETYTITARMDPAVAIETSPPAMTLRSGSSRKFSVS 780
           SPSITIAHLVGT+ VTRTVTNVAEEETYTITARMDPAVAIE +PPAMTLRSGSSRKFSV+
Sbjct: 721 SPSITIAHLVGTKIVTRTVTNVAEEETYTITARMDPAVAIEVNPPAMTLRSGSSRKFSVT 780

Query: 781 LTARSLTGTYSFGEVLLKGSRGHKVRIPVVAMGYQR 817
           LTARSLTGTYSFG+VLLKGSRGHKVRIPVVAMGYQR
Sbjct: 781 LTARSLTGTYSFGQVLLKGSRGHKVRIPVVAMGYQR 816

BLAST of Lag0006242 vs. TAIR 10
Match: AT2G19170.1 (subtilisin-like serine protease 3 )

HSP 1 Score: 1353.6 bits (3502), Expect = 0.0e+00
Identity = 664/809 (82.08%), Postives = 727/809 (89.86%), Query Frame = 0

Query: 9   VLVFFALLVVGKAEIYIVTIDGEPIVSYKGDLDGFEATALESDEKIDPTSEIVTSYARHL 68
           + V F LLV   AE+YIVT++G+PI+SYKG  +GFEATA+ESDEKID +SE+VT YARHL
Sbjct: 7   IFVVFVLLVAVTAEVYIVTMEGDPIISYKGGENGFEATAVESDEKIDTSSELVTVYARHL 66

Query: 69  ENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDISPEQAEILRHTPIVKSVERDWKVRKL 128
           E KHDM+LGMLFE GS+KKLYSYKHLINGFA  +SPEQAE LR  P V+SV++DWKVR+L
Sbjct: 67  ERKHDMILGMLFEEGSYKKLYSYKHLINGFAAHVSPEQAETLRRAPGVRSVDKDWKVRRL 126

Query: 129 TTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPFGPCSKYKG 188
           TTHTPEFLGLPT VWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFA+++  P+GP   YKG
Sbjct: 127 TTHTPEFLGLPTDVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFASHHRLPYGPLPHYKG 186

Query: 189 KCEVDPDTKKDFCNGKIIGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHTAAIAAGNN 248
           KCE DP TKK FCN KI+GAQHFAEAAKAAGAFNP I +ASP+DGDGHGSHTAAIAAGNN
Sbjct: 187 KCEEDPHTKKSFCNRKIVGAQHFAEAAKAAGAFNPDIDYASPMDGDGHGSHTAAIAAGNN 246

Query: 249 GIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGVDILSLSVG 308
           GIP+RMHGYEFGKASGMAPRARIAVYKALYR+FGGFVADVVAAIDQAVHDGVDILSLSVG
Sbjct: 247 GIPLRMHGYEFGKASGMAPRARIAVYKALYRLFGGFVADVVAAIDQAVHDGVDILSLSVG 306

Query: 309 PNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIATVAAAIDD 368
           PNSPP TTK T+LNPFDATLL AVKAGVFVAQAAGNGGPFPKTLVSYSPWI TVAAAIDD
Sbjct: 307 PNSPPTTTKTTFLNPFDATLLGAVKAGVFVAQAAGNGGPFPKTLVSYSPWITTVAAAIDD 366

Query: 369 RRYKNHLKLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVMKYSPSDCQRPEVLNK 428
           RRYKNHL LGNGK+LAG+GLSP T  +R YTLV+ANDVLLDSSV KY+PSDCQRPEV NK
Sbjct: 367 RRYKNHLTLGNGKMLAGMGLSPPTRPHRLYTLVSANDVLLDSSVSKYNPSDCQRPEVFNK 426

Query: 429 RLVEGKVLLCGYSFSFVVGTASIKKVSQTAKALGAAGFVLAVENISPGAKFDPVPVGIPG 488
           +LVEG +LLCGYSF+FVVGTASIKKV  TAK LGAAGFVL VEN+SPG KFDPVP  IPG
Sbjct: 427 KLVEGNILLCGYSFNFVVGTASIKKVVATAKHLGAAGFVLVVENVSPGTKFDPVPSAIPG 486

Query: 489 ILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEVALFSARGP 548
           ILITDVSKSMDLIDYYN ST RDWTGRVKSF A GSIGDGL P+L+KSAP+VALFSARGP
Sbjct: 487 ILITDVSKSMDLIDYYNASTSRDWTGRVKSFKAEGSIGDGLAPVLHKSAPQVALFSARGP 546

Query: 549 NIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMAAPHIAGIA 608
           N +DFSFQDADLLKPDILAPG LIWAAW PNGTDEPNYVGEGFA+ISGTSMAAPHIAGIA
Sbjct: 547 NTKDFSFQDADLLKPDILAPGYLIWAAWCPNGTDEPNYVGEGFALISGTSMAAPHIAGIA 606

Query: 609 ALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLVTATPFDYGSGHVN 668
           ALVKQKHP WSPAAIKSALMTTST +DRAGR L+AQQ+S+TEA+ LV ATPFDYGSGHVN
Sbjct: 607 ALVKQKHPQWSPAAIKSALMTTSTVIDRAGRLLQAQQYSDTEAVTLVKATPFDYGSGHVN 666

Query: 669 PRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTKTPCNFTMGHPWNLNSPSITIAH 728
           P AALDPGLIFDAGYEDYLGFLCTT GI+ HEI NYT T CN+ M HP N N+PSI ++H
Sbjct: 667 PSAALDPGLIFDAGYEDYLGFLCTTPGISAHEIRNYTNTACNYDMKHPSNFNAPSIAVSH 726

Query: 729 LVGTQTVTRTVTNVAE-EETYTITARMDPAVAIETSPPAMTLRSGSSRKFSVSLTARSLT 788
           LVGTQTVTR VTNVAE EETYTITARM P++AIE +PPAMTLR G++R FSV++T RS++
Sbjct: 727 LVGTQTVTRKVTNVAEVEETYTITARMQPSIAIEVNPPAMTLRPGATRTFSVTMTVRSVS 786

Query: 789 GTYSFGEVLLKGSRGHKVRIPVVAMGYQR 817
           G YSFGEV LKGSRGHKVRIPVVA+G++R
Sbjct: 787 GVYSFGEVKLKGSRGHKVRIPVVALGHRR 815

BLAST of Lag0006242 vs. TAIR 10
Match: AT4G30020.1 (PA-domain containing subtilase family protein )

HSP 1 Score: 1347.4 bits (3486), Expect = 0.0e+00
Identity = 664/810 (81.98%), Postives = 730/810 (90.12%), Query Frame = 0

Query: 9   VLVFFALLVVGKAEIYIVTIDGEPIVSYKGDLDGFEATALESDEKIDPTSEIVTSYARHL 68
           VLVFF   +   AEIYIVT++GEPI+SYKG  +GFEATA+ESDEKID TSE+VTSYARHL
Sbjct: 7   VLVFFTCFLTVTAEIYIVTMEGEPIISYKGGDNGFEATAVESDEKIDTTSELVTSYARHL 66

Query: 69  ENKHDMLLGMLFERGSFKKLYSYKHLINGFAVDISPEQAEILRHTPIVKSVERDWKVRKL 128
           E KHDMLLGMLF  GS+KKLYSYKHLINGFA  +SP+QAE+LR  P VKSV+RDWKVRKL
Sbjct: 67  ERKHDMLLGMLFVEGSYKKLYSYKHLINGFAAHVSPDQAEMLRRAPGVKSVDRDWKVRKL 126

Query: 129 TTHTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTE-PFGPCSKYK 188
           TTHTP+FLGLPT VWPTGGG+DRAGEDIVIGF+DSGI+PHHPSFA+++T  P+GP   YK
Sbjct: 127 TTHTPQFLGLPTDVWPTGGGYDRAGEDIVIGFIDSGIFPHHPSFASHHTTVPYGPHPSYK 186

Query: 189 GKCEVDPDTKKDFCNGKIIGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHTAAIAAGN 248
           GKCE DP TK  FCNGKIIGAQHFAEAAKAAGAFNP I FASP+DGDGHGSHTAAIAAGN
Sbjct: 187 GKCEEDPHTKISFCNGKIIGAQHFAEAAKAAGAFNPDIDFASPMDGDGHGSHTAAIAAGN 246

Query: 249 NGIPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGVDILSLSV 308
           NGIPVRMHGYEFGKASGMAPRARIAVYKALYR+FGGFVADVVAAIDQAVHDGVDILSLSV
Sbjct: 247 NGIPVRMHGYEFGKASGMAPRARIAVYKALYRLFGGFVADVVAAIDQAVHDGVDILSLSV 306

Query: 309 GPNSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIATVAAAID 368
           GPNSPPATTK T+LNPFDATLL AVKAGVFVAQAAGNGGPFPKTLVSYSPWI TVAAAID
Sbjct: 307 GPNSPPATTKTTFLNPFDATLLGAVKAGVFVAQAAGNGGPFPKTLVSYSPWITTVAAAID 366

Query: 369 DRRYKNHLKLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVMKYSPSDCQRPEVLN 428
           DRRYKNHL LGNGK+LAG+GLSP+T  +R+Y +V+ANDVLL SS MKY+PSDCQ+PEVLN
Sbjct: 367 DRRYKNHLTLGNGKMLAGIGLSPSTRPHRSYKMVSANDVLLGSSGMKYNPSDCQKPEVLN 426

Query: 429 KRLVEGKVLLCGYSFSFVVGTASIKKVSQTAKALGAAGFVLAVENISPGAKFDPVPVGIP 488
           K+LVEG +LLCGYSF+FV G+ASIKKV++TAK LGAAGFVL VEN+SPG KFDPVP  IP
Sbjct: 427 KKLVEGNILLCGYSFNFVAGSASIKKVAETAKHLGAAGFVLVVENVSPGTKFDPVPSCIP 486

Query: 489 GILITDVSKSMDLIDYYNTSTPRDWTGRVKSFDAVGSIGDGLMPLLYKSAPEVALFSARG 548
           GILITDVSKSMDLIDYYN +T RDW GRVK F A GSIGDGL P+L+KSAPEVALFSARG
Sbjct: 487 GILITDVSKSMDLIDYYNVTTSRDWMGRVKDFKAEGSIGDGLEPILHKSAPEVALFSARG 546

Query: 549 PNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMAAPHIAGI 608
           PN +DFSFQDADLLKPDILAPGSLIW+AWS NGTDE NY+GEGFA+ISGTSMAAPHIAGI
Sbjct: 547 PNTKDFSFQDADLLKPDILAPGSLIWSAWSANGTDEANYIGEGFALISGTSMAAPHIAGI 606

Query: 609 AALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLVTATPFDYGSGHV 668
           AALVKQKHP WSPAAIKSALMTTST +DRAGRPL+AQQ+SETE + LV ATPFDYGSGHV
Sbjct: 607 AALVKQKHPQWSPAAIKSALMTTSTVIDRAGRPLQAQQYSETETVTLVKATPFDYGSGHV 666

Query: 669 NPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTKTPCNFTMGHPWNLNSPSITIA 728
           NP AALDPGLIFDAGYEDY+GFLCTT GI+ HEI N+T TPCNF M HP N N+PSI I+
Sbjct: 667 NPSAALDPGLIFDAGYEDYIGFLCTTPGIDAHEIKNFTNTPCNFKMVHPSNFNTPSIAIS 726

Query: 729 HLVGTQTVTRTVTNVA-EEETYTITARMDPAVAIETSPPAMTLRSGSSRKFSVSLTARSL 788
           HLV TQTVTR VTNVA EEETYTIT+RM+PA+AIE SPPAMT+R+G+SR FSV+LT RS+
Sbjct: 727 HLVRTQTVTRRVTNVAEEEETYTITSRMEPAIAIEVSPPAMTVRAGASRTFSVTLTVRSV 786

Query: 789 TGTYSFGEVLLKGSRGHKVRIPVVAMGYQR 817
           TG YSFG+V LKGSRGHKV +PVVAMG +R
Sbjct: 787 TGAYSFGQVTLKGSRGHKVTLPVVAMGQRR 816

BLAST of Lag0006242 vs. TAIR 10
Match: AT5G44530.1 (Subtilase family protein )

HSP 1 Score: 661.0 bits (1704), Expect = 1.2e-189
Identity = 371/810 (45.80%), Postives = 502/810 (61.98%), Query Frame = 0

Query: 23  IYIVTIDGEPIVSYKGDLDGFEATALESDE-----KIDPTSEIVTSYARHLENK------ 82
           +YIVT+   PIV        FE   L+  +     K+ P +    S  RH ++K      
Sbjct: 38  VYIVTLKQPPIVHL------FEEQELKHKKSKFTPKLRPRN---NSRKRHGKSKIPSVVQ 97

Query: 83  -HDMLLGMLFERGSFKKLYSYKHLINGFAVDISPEQAEILRHTPIVKSVERDWKVRKLTT 142
            HD  L    +   + KLYSY +LINGFA+ I+ +QAE L     V ++  D+ VR  TT
Sbjct: 98  SHDSFLRKTLKGEKYIKLYSYHYLINGFALFINSQQAEKLSMRKEVANIVLDYSVRTATT 157

Query: 143 HTPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNTEPFGPCSK-YKGK 202
           +TP+F+GLP G W   GGF+ AGE ++IGF+D+GI P+HPSF   +++   P  K + G 
Sbjct: 158 YTPQFMGLPQGAWVKEGGFEIAGEGVIIGFIDTGIDPNHPSFNDNDSKRSYPIPKHFSGV 217

Query: 203 CEVDPDTKKDFCNGKIIGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHTAAIAAGNNG 262
           CEV PD     CN K+IGA+HFA++A   G FN +  +ASP DGDGHG+HTA++AAGN+G
Sbjct: 218 CEVTPDFPSGSCNKKLIGARHFAQSAVTRGIFNSSEDYASPFDGDGHGTHTASVAAGNHG 277

Query: 263 IPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGVDILSLSVGP 322
           +PV +  + FG ASG+APRA I+VYKALY+ FGGF ADVVAAIDQA  DGVDILSLS+ P
Sbjct: 278 VPVIVSNHNFGYASGIAPRAFISVYKALYKSFGGFAADVVAAIDQAAQDGVDILSLSITP 337

Query: 323 NSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIATVAAAIDDR 382
           N  P     T+ NP D  LLSAVKAG+FV QAAGN GP PKT+ S+SPWI TV A+  DR
Sbjct: 338 NRKPPGV-ATFFNPIDMALLSAVKAGIFVVQAAGNTGPAPKTMSSFSPWIFTVGASSHDR 397

Query: 383 RYKNHLKLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVMKYS--PSDCQRPEVLN 442
            Y N L LGN   + G+G +  T   + Y +++A   L +S+ +       +CQ  E  +
Sbjct: 398 VYSNSLTLGNNVTIPGMGFAIPTDSGKMYKMISAFHALNNSTSVDKDMYVGECQDYENFD 457

Query: 443 KRLVEGKVLLCGYSFSFVVGTASIKKVSQTAKALGAAGFVLAVENISPGAKFDPVPVGIP 502
           +  V GK+L+C YS  FV+G ++IK+    AK L A G +  ++    G + +P P+ +P
Sbjct: 458 QDRVSGKLLICSYSARFVLGLSTIKQALDVAKNLSATGVIFYIDPYVLGFEINPTPMDMP 517

Query: 503 GILITDVSKSMDLIDYYNTSTPRDWTGR-VKSFDAVGSIGDGLMPLLYKSAPEVALFSAR 562
           GI+I  V  S  L+ YYN+S  RD T + + SF AV +I  GL       AP+V  +SAR
Sbjct: 518 GIIIPSVEDSKTLLKYYNSSIQRDVTTKEIVSFGAVAAIEGGLNANFSNRAPKVMYYSAR 577

Query: 563 GPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMAAPHIAG 622
           GP+  D SF DAD+LKP+++APG+ IW AWS   TD   + GE FAM+SGTSMAAPH+AG
Sbjct: 578 GPDPEDNSFNDADVLKPNLVAPGNSIWGAWSSASTDSTEFEGEKFAMMSGTSMAAPHVAG 637

Query: 623 IAALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLVTATPFDYGSGH 682
           +AAL+KQ +P ++P+ I SAL TT+   D  G P+ AQ+        L TATP D GSG 
Sbjct: 638 VAALIKQSYPQFTPSTISSALSTTALLNDNKGSPIMAQRTYSNPDQSLYTATPSDMGSGF 697

Query: 683 VNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHE--IHNYT--KTPCNFTMGHPWNLNSP 742
           VN  AALDPGL+FD  +EDY+ FLC   GIN  +  + NYT  + P N T    ++LN P
Sbjct: 698 VNATAALDPGLVFDTSFEDYISFLC---GINGSDTVVFNYTGFRCPANNTPVSGFDLNLP 757

Query: 743 SITIAHLVGTQTVTRTVTNVAEEETYTITARMDPAVAIETSPPAMTLRSGSSRKFSVSLT 802
           SIT++ L GTQT  R++ N+A  ETY +       V+++ SP   ++  G ++  SV+LT
Sbjct: 758 SITVSTLSGTQTFQRSMRNIAGNETYNVGWSPPYGVSMKVSPTQFSIAMGENQVLSVTLT 817

Query: 803 ARSLTGTYSFGEVLLKGSRGHKVRIPVVAM 813
               + + SFG + L G+ GH V IPV  +
Sbjct: 818 VTKNSSSSSFGRIGLFGNTGHIVNIPVTVI 834

BLAST of Lag0006242 vs. TAIR 10
Match: AT4G20430.1 (Subtilase family protein )

HSP 1 Score: 649.4 bits (1674), Expect = 3.7e-186
Identity = 355/747 (47.52%), Postives = 472/747 (63.19%), Query Frame = 0

Query: 72  HDMLLGMLFERGSFKKLYSYKHLINGFAVDISPEQAEILRHTPIVKSVERDWKVRKLTTH 131
           HD LL    +   + KLYS+ +LINGFAV +S +QAE L     V ++  D+ VR  TT+
Sbjct: 103 HDSLLRNALKGEKYIKLYSFHYLINGFAVFVSSQQAETLSRRREVANIVLDFSVRTATTY 162

Query: 132 TPEFLGLPTGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFATYNT--EPFGPCSKYKGK 191
           TP+F+GLP G W   GG++ AGE IVIGF+D+GI P HPSF   +T    +   + + G 
Sbjct: 163 TPQFMGLPKGAWVKEGGYETAGEGIVIGFIDTGIDPTHPSFNGTDTSQRQYPIPNHFSGV 222

Query: 192 CEVDPDTKKDFCNGKIIGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHTAAIAAGNNG 251
           CEV PD     CN K++GA+HFA++A   G FN +  +ASP DGDGHG+HTA+IAAGN+G
Sbjct: 223 CEVTPDFPSGSCNRKLVGARHFAQSAITRGIFNSSEDYASPFDGDGHGTHTASIAAGNHG 282

Query: 252 IPVRMHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGVDILSLSVGP 311
           +   + G+ FG ASG+APRA I+VYKALY+ FGGF ADVVAAIDQA  DGVDILSLS+ P
Sbjct: 283 VSAVVSGHNFGSASGIAPRAHISVYKALYKSFGGFAADVVAAIDQAAQDGVDILSLSITP 342

Query: 312 NSPPATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIATVAAAIDDR 371
           N  P     T+ NP D  +LSAVKAG+FV QAAGN GP PK++ S+SPWI TV AA  DR
Sbjct: 343 NRRPPGV-ATFFNPLDMAMLSAVKAGIFVVQAAGNTGPSPKSMSSFSPWIFTVGAASHDR 402

Query: 372 RYKNHLKLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVM---KYSPSDCQRPEVL 431
            Y N + LGN   + G+GL+  T   + YT+++A D L + S +        +CQ     
Sbjct: 403 DYSNSIVLGNNVSIPGVGLALRTDEGKKYTMISALDALKNKSSVVDKDMYVGECQDYGSF 462

Query: 432 NKRLVEGKVLLCGYSFSFVVGTASIKKVSQTAKALGAAGFVLAVENISPGAKFDPVPVGI 491
           +K ++ G +L+C YS  FV+G ++IK+    AK L A G V  ++    G + +P P+ +
Sbjct: 463 DKDVIRGNLLICSYSIRFVLGLSTIKQALAVAKNLSAKGVVFYMDPYVLGFQINPTPMDM 522

Query: 492 PGILITDVSKSMDLIDYYNTSTPRDWTGR-VKSFDAVGSIGDGLMPLLYKSAPEVALFSA 551
           PGI+I     S  L+ YYN+S  RD T + +  F AV +I  G        AP++  +SA
Sbjct: 523 PGIIIPSAEDSKVLLKYYNSSLVRDGTTKEIVRFGAVAAIAGGQNANFSNRAPKIMYYSA 582

Query: 552 RGPNIRDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMAAPHIA 611
           RGP+ +D  F DAD+LKP+++APG+ IW AWS   T+   + GE FAM+SGTSMAAPH+A
Sbjct: 583 RGPDPQDSLFNDADILKPNLVAPGNSIWGAWSSAATESTEFEGESFAMMSGTSMAAPHVA 642

Query: 612 GIAALVKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLVTATPFDYGSG 671
           G+AALVKQK   +SP+AI SAL TTS   D  G  + AQ+        +  ATPFD G+G
Sbjct: 643 GVAALVKQKFRKFSPSAIASALSTTSVLFDNKGEAIMAQRAYANPDQTISPATPFDMGNG 702

Query: 672 HVNPRAALDPGLIFDAGYEDYLGFLCTTAGINVHEIHNYTKTPC---NFTMGHPWNLNSP 731
            VN  AALDPGLIFD  +EDY+ FLC   G +   + NYT T C   N T+    +LN P
Sbjct: 703 FVNATAALDPGLIFDTSFEDYMSFLCGING-SAPVVFNYTGTNCLRNNATISGS-DLNLP 762

Query: 732 SITIAHLVGTQTVTRTVTNVAEEETYTITARMDPAVAIETSPPAMTLRSGSSRKFSVSLT 791
           SIT++ L  T+TV R +TN+A  ETYT++      V I  SP   ++ SG ++  SV LT
Sbjct: 763 SITVSKLNNTRTVQRLMTNIAGNETYTVSLITPFDVLINVSPTQFSIASGETKLLSVILT 822

Query: 792 ARSLTGTYSFGEVLLKGSRGHKVRIPV 810
           A+  +   SFG + L G+ GH VRIPV
Sbjct: 823 AKRNSSISSFGGIKLLGNAGHIVRIPV 846

BLAST of Lag0006242 vs. TAIR 10
Match: AT1G30600.1 (Subtilase family protein )

HSP 1 Score: 633.6 bits (1633), Expect = 2.1e-181
Identity = 363/810 (44.81%), Postives = 497/810 (61.36%), Query Frame = 0

Query: 23  IYIVTIDGEPIVSYKGDLDGFEATALESDEKIDPTSEIV---TSYARHLENKHDMLLGML 82
           +YIVT+   P V + G        + +S   +  TS  +    + +  +   HD LL  +
Sbjct: 36  VYIVTLKDRPSVHFSG------RESSDSKHSLTATSSQIYRTLNRSASIIRVHDSLLRNV 95

Query: 83  FERGSFKKLYSYKHLINGFAVDISPEQAEILRHTPIVKSVERDWKVRKLTTHTPEFLGLP 142
             + ++ KLYSY +LINGF+  ++ +QA+ L     V++V  D+ V K TTHTP+FLGLP
Sbjct: 96  LRKENYLKLYSYHYLINGFSAVLTRKQADRLAAREEVENVVLDFLVEKATTHTPQFLGLP 155

Query: 143 TGVWPTGGGFDRAGEDIVIGFVDSGIYPHHPSFA------TYNTEPFGPCSKYKGKCEVD 202
            G W   GG + AGE +VIGF+D+GI P HPSF+      TY+  P      + G CEV 
Sbjct: 156 RGAWLRDGGSEYAGEGVVIGFIDTGIDPTHPSFSDKISGHTYSVPP-----HFTGVCEVT 215

Query: 203 PDTKKDFCNGKIIGAQHFAEAAKAAGAFNPAIHFASPLDGDGHGSHTAAIAAGNNGIPVR 262
                  CN K+IGA+HFAE+A + G  N +   ASP DG+GHG+HTA++AAGN+GIPV 
Sbjct: 216 IGFPPGSCNRKLIGARHFAESALSRGVLNSSQDDASPFDGEGHGTHTASVAAGNHGIPVV 275

Query: 263 MHGYEFGKASGMAPRARIAVYKALYRIFGGFVADVVAAIDQAVHDGVDILSLSVGPNSPP 322
           + G+  G ASGMAPRA IA+YKALY+ FGGF AD++AAIDQA  DGVDI++LS+ PN  P
Sbjct: 276 VAGHRLGNASGMAPRAHIAIYKALYKRFGGFAADIIAAIDQAAQDGVDIINLSITPNRRP 335

Query: 323 ATTKITYLNPFDATLLSAVKAGVFVAQAAGNGGPFPKTLVSYSPWIATVAAAIDDRRYKN 382
                T+ NP D  LLSAVKAG+FV QAAGN GP PK++ S+SPWI TV A   DR Y N
Sbjct: 336 PGI-ATFFNPIDMALLSAVKAGIFVVQAAGNTGPAPKSMSSFSPWIFTVGATSHDRVYSN 395

Query: 383 HLKLGNGKILAGLGLSPATHLNRTYTLVAANDVLLDSSVMKYS--PSDCQRPEVLNKRLV 442
            + LGN   + G+GL+  T +   + LV A   L + + +  +    +CQ     +++LV
Sbjct: 396 SIILGNNVTIPGVGLASGTRI--MHKLVLATHALRNGTTVMDAIYVGECQDSSSFDQKLV 455

Query: 443 EGKVLLCGYSFSFVVGTASIKKVSQTAKALGAAGFVLAVENISPGAKFDPVPVGIPGILI 502
           +GK+L+C Y+  F++G ++IK+   TAK L AAG V  ++  + G +    P+ IPGILI
Sbjct: 456 QGKILVCSYTVRFILGVSTIKQALLTAKNLTAAGLVFYIDPSATGFQMTSSPMDIPGILI 515

Query: 503 TDVSKSMDLIDYYNTSTPRD-WTGRVKSFDAVGSIGDGLMPLLYKSAPEVALFSARGPNI 562
           +    S  L+ YYN+S  R+  +G++    +V  I  G+ P    +AP+V  FSARGP+ 
Sbjct: 516 SSPQDSQALLRYYNSSLLRENGSGKIVGSASVAKIVGGMRPTYGITAPKVMYFSARGPDP 575

Query: 563 RDFSFQDADLLKPDILAPGSLIWAAWSPNGTDEPNYVGEGFAMISGTSMAAPHIAGIAAL 622
            D SF DAD++KP+++APG+ IW AWSP G    ++ GE FAM SGTSM+APH+ GIAAL
Sbjct: 576 EDDSFVDADIMKPNLVAPGNAIWGAWSPLGIGTNDFQGERFAMESGTSMSAPHVTGIAAL 635

Query: 623 VKQKHPNWSPAAIKSALMTTSTTMDRAGRPLKAQQFSETEAMKLVTATPFDYGSGHVNPR 682
           +KQK P+++PAAI SAL TT++  DR G  + AQ+      +    ATPFD GSG VN  
Sbjct: 636 IKQKFPHFTPAAIASALSTTASLSDRKGEHIMAQRTVLNPDISQSPATPFDMGSGFVNAT 695

Query: 683 AALDPGLIFDAGYEDYLGFLCTTAGINVHE--IHNYTKTPC---NFTMGHPWNLNSPSIT 742
           AALDPGLIFD GY +Y+ FLC   GIN     + NYT   C   N ++    +LN PS+T
Sbjct: 696 AALDPGLIFDIGYNEYMKFLC---GINGSSPVVLNYTGESCSSYNSSLAAS-DLNLPSVT 755

Query: 743 IAHLVGTQTVTRTVTNV---AEEETYTITARMDPAVAIETSPPAMTLRSGSSRKFSVSLT 802
           IA LVGT+ V R VTN+   A  ETY +      +V+++ SP   T+ +G +R  S+   
Sbjct: 756 IAKLVGTRAVLRWVTNIATTATNETYIVGWMAPDSVSVKVSPAKFTIGNGQTRVLSLVFR 815

Query: 803 ARSLTGTYSFGEVLLKGSRGHKVRIPVVAM 813
           A       SFG + L G RGH V IPV  +
Sbjct: 816 AMKNVSMASFGRIGLFGDRGHVVNIPVAVI 827

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038890136.10.0e+0096.94subtilisin-like protease SBT2.5 isoform X1 [Benincasa hispida] >XP_038890137.1 s... [more]
XP_023546345.10.0e+0096.57subtilisin-like protease SBT2.5 [Cucurbita pepo subsp. pepo] >XP_023546347.1 sub... [more]
XP_022996483.10.0e+0096.57subtilisin-like protease SBT2.5 isoform X1 [Cucurbita maxima] >XP_022996484.1 su... [more]
XP_022929689.10.0e+0096.32subtilisin-like protease SBT2.5 [Cucurbita moschata] >XP_022929690.1 subtilisin-... [more]
KAG6598892.10.0e+0096.32Subtilisin-like protease 2.5, partial [Cucurbita argyrosperma subsp. sororia] >K... [more]
Match NameE-valueIdentityDescription
O644810.0e+0082.08Subtilisin-like protease SBT2.5 OS=Arabidopsis thaliana OX=3702 GN=SBT2.5 PE=2 S... [more]
Q9SZV50.0e+0081.98Subtilisin-like protease SBT2.6 OS=Arabidopsis thaliana OX=3702 GN=SBT2.6 PE=2 S... [more]
Q9FI121.8e-18845.80Subtilisin-like protease SBT2.3 OS=Arabidopsis thaliana OX=3702 GN=SBT2.3 PE=2 S... [more]
Q9SUN65.3e-18547.52Subtilisin-like protease SBT2.2 OS=Arabidopsis thaliana OX=3702 GN=SBT2.2 PE=3 S... [more]
Q9SA753.0e-18044.81Subtilisin-like protease SBT2.1 OS=Arabidopsis thaliana OX=3702 GN=SBT2.1 PE=2 S... [more]
Match NameE-valueIdentityDescription
A0A6J1KAV80.0e+0096.57subtilisin-like protease SBT2.5 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
A0A6J1EPH60.0e+0096.32subtilisin-like protease SBT2.5 OS=Cucurbita moschata OX=3662 GN=LOC111436195 PE... [more]
A0A5D3CEZ70.0e+0096.45Subtilisin-like protease SBT2.5 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_... [more]
A0A1S3BRZ60.0e+0096.45subtilisin-like protease SBT2.5 OS=Cucumis melo OX=3656 GN=LOC103492606 PE=3 SV=... [more]
A0A5A7SQC10.0e+0096.45Subtilisin-like protease SBT2.5 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
Match NameE-valueIdentityDescription
AT2G19170.10.0e+0082.08subtilisin-like serine protease 3 [more]
AT4G30020.10.0e+0081.98PA-domain containing subtilase family protein [more]
AT5G44530.11.2e-18945.80Subtilase family protein [more]
AT4G20430.13.7e-18647.52Subtilase family protein [more]
AT1G30600.12.1e-18144.81Subtilase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR015500Peptidase S8, subtilisin-relatedPRINTSPR00723SUBTILISINcoord: 232..245
score: 42.71
coord: 153..172
score: 33.74
coord: 595..611
score: 64.03
IPR010259Peptidase S8 propeptide/proteinase inhibitor I9PFAMPF05922Inhibitor_I9coord: 24..126
e-value: 1.2E-16
score: 61.2
IPR036852Peptidase S8/S53 domain superfamilyGENE3D3.40.50.200Peptidase S8/S53 domaincoord: 155..686
e-value: 2.4E-141
score: 473.7
IPR036852Peptidase S8/S53 domain superfamilySUPERFAMILY52743Subtilisin-likecoord: 128..677
NoneNo IPR availableGENE3D2.60.40.2310coord: 690..813
e-value: 5.5E-18
score: 66.9
NoneNo IPR availableGENE3D3.50.30.30coord: 368..533
e-value: 2.4E-141
score: 473.7
NoneNo IPR availablePANTHERPTHR10795:SF601CUCUMISIN-RELATEDcoord: 14..815
NoneNo IPR availablePROSITEPS51892SUBTILASEcoord: 122..673
score: 28.389046
NoneNo IPR availableCDDcd02120PA_subtilisin_likecoord: 375..513
e-value: 2.62784E-16
score: 73.9871
NoneNo IPR availableSUPERFAMILY54897Protease propeptides/inhibitorscoord: 85..126
IPR037045Peptidase S8 propeptide/proteinase inhibitor I9 superfamilyGENE3D3.30.70.80Peptidase S8 propeptide/proteinase inhibitor I9coord: 19..128
e-value: 1.8E-6
score: 30.3
IPR000209Peptidase S8/S53 domainPFAMPF00082Peptidase_S8coord: 153..634
e-value: 7.1E-43
score: 146.9
IPR041469Subtilisin-like protease, fibronectin type-III domainPFAMPF17766fn3_6coord: 718..810
e-value: 1.1E-15
score: 57.6
IPR045051Subtilisin-like proteasePANTHERPTHR10795PROPROTEIN CONVERTASE SUBTILISIN/KEXINcoord: 14..815
IPR023827Peptidase S8, subtilisin, Asp-active sitePROSITEPS00136SUBTILASE_ASPcoord: 158..169
IPR023828Peptidase S8, subtilisin, Ser-active sitePROSITEPS00138SUBTILASE_SERcoord: 596..606
IPR034197Cucumisin-like catalytic domainCDDcd04852Peptidases_S8_3coord: 129..632
e-value: 9.08632E-122
score: 367.308

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0006242.1Lag0006242.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
biological_process GO:0009610 response to symbiotic fungus
molecular_function GO:0004252 serine-type endopeptidase activity
molecular_function GO:0008236 serine-type peptidase activity