Lag0006232 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0006232
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionDNA ligase
Locationchr6: 39690331 .. 39692613 (-)
RNA-Seq ExpressionLag0006232
SyntenyLag0006232
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATTCTGAGACACCATGGGCCTCGAAACATGTCTCGAAACATATAGAAAACCAGAAAATTTCCTCTCCTTCCAAGGTGTATAAAGATCGAGGAAGAAACGATGCTGCTTCAGGACAACACATTCTCTCATATTCAACTGAGAGATCTAGTGGAAACAAAAGAGGAAGGCCTGCTGCAAGGAGTATGCAGAAAGGGAAATCGGGTGTCGAACAAGTTCGAAGAGCTCGGGCACGAACAGCCAAGGGGCCTGCAAAAATAAGTGAAGTTGATTCAGACATCAGTGACCATACTGATGAAAAAACGAATGCAGAAACTGGAAATATTAGAACACTAAACAGTGAAAACTCAGAAAAGGAGTTTAAAATGCTAGAAAATGAGAGACCCGACAAGAAGAAAAGAGGAAGACCTACTACAAGGAACACACAGAAAGGGAAAGCAGGGGTCGAACAAGTTCGAAGAACTCGGGCACGTATAGCCAAGGGGTCAGGTAAAATAGGCGATGTTGAGTCAGACAGCAGTGACTCTACTGGTGAAAAACCAAAAGCGGAAGAAACTGGAATGGATAAAGGAAACATTGGAACAGTAAGCGAGGAAAACGCAAAAACAGATGAGTTCAATATGCTAGAAGACCGTGATTGGAAACAAAAAGGCAAGGCCATTGAACAAATTGATGTTGCAGAACCCAAATCTAATGTTCAGTTTGGTAAACTTCCAGAAGTCAATATATCTGAAAGGTATAATTACCAAGACACTGAAAAATCTGAAAAGCTGGAAGTCATGACCGATCCACTTCAAGCTATGTTATTAGATATGGTTCCAAGTCTTGGAATGAGTGAAACTAAAAGTAGTAGTACTGTTCTTGAGGAGAAGCAACCACCAGTACACAACAATTCTGAGCCAGCTAAGAAGAAAGTCAGCTACAAGGACGTTGCCAGTGAACTGCTTAAGGATTGGTAAAACGTGTGGTTTTTACTGGTTTGTCTATGTGTTTCTAATGTGATAGACTGACAAAAAGTCATCTAAGTATCATGTTGTAATTAAATCGCTGCTGTTCAATCATTTCAACACAAGGTATTTAATTTATGGTGAGTTTAGGACACTTAAAAAGAGTTATTAAGTGTTTTTTAAATATACTTTTGATAGTGTATTGTATCTGATCTCATGAAAGATTTAGAAATATTTTAAATTATTTTAAAATATTTTCCACTGAAAGTCATGTCAAAATTAGTCTTGAATGGGTTGGTTTTTCATTTATGGAGGCCAACTTTGTGAGTCTTGCAATGTGTCTTGAAATCCATTGTTTTTGTAACGCAAAATGTGTTATGGTTAGAGGAAGAAGTTGAAGATGGAGGAAAAGATGAAGTTGATGGAGATGAAGATGGAGGAAGAAGATGATGATGAGTATGGAGATGATGAAGATGAAAAAAGAAAAAGAAAAAAAAAAGAATAATAAAATATCAGTTAAACTATTTTTATTTAAAAAAATAAAAAATATATTTTTTTTAATAAAAAAATGCACCTCACGTCAACAATCTGTTAGGGTTTAATGAAAATTTGGATGAAAGGACCAACAATGTAGTGAAATCCAAACCTCATAAGCAAGAATGGAGTTTGAAAACTTAGGGAACAAATGAAATCTAATTCAAACAATATTTTTTTCTTAGGTTGTGGAAAGTTCAACAAACACGTAATTATTTTTCTAGAATAATCTAACACCAGAATGGTTATTGATAGCAACTTTGTTCTCGTTGCTTCCTCTAGTTTTATGCCATGCGCATTTTCTGTTTTGTATTTAATTTGTTCAATTTGTTATTCCAAACCCTTAATTGATTCTATAAATTTAGTCATTTCTCAACATTCAATACACAGAAGAGAATAGTGCATTCATCCTCCATTTTATTTGCAATTGTTTTCTTGATATCAAAGCTCGACTATGCCCAATGCCTCATCTAATTTTAGTTCTTCGACAAATGTAACCTTTAGAAGTCCTCTCAACAAATTGCTATCATCAAGTTAGACCAGGGAAATTTTCTTTTCTGGAAAAATCTTGCGTTGCTCATATTACGTAGTTACAAGTTAGAAGGCCACCTTTCCAGCAAAAAGCCATGTCCTCCAACAAAAATTTCAGCATCAAATGTAGCTGAAAATTCAGGAGCCTCAGCAGAAGCAGATGTCTTTGGAGTATCAAGTTCAAGACCTGCAGTGACAGCAGTAGTTAACCCACTTTATGAATCCTGGATAGCTATTGATCAGCTTCTTCTTAGCTGGCTATATAATTAA

mRNA sequence

ATGGATTCTGAGACACCATGGGCCTCGAAACATGTCTCGAAACATATAGAAAACCAGAAAATTTCCTCTCCTTCCAAGGTGTATAAAGATCGAGGAAGAAACGATGCTGCTTCAGGACAACACATTCTCTCATATTCAACTGAGAGATCTAGTGGAAACAAAAGAGGAAGGCCTGCTGCAAGGAGTATGCAGAAAGGGAAATCGGGTGTCGAACAAGTTCGAAGAGCTCGGGCACGAACAGCCAAGGGGCCTGCAAAAATAAGTGAAGTTGATTCAGACATCAGTGACCATACTGATGAAAAAACGAATGCAGAAACTGGAAATATTAGAACACTAAACAGTGAAAACTCAGAAAAGGAGTTTAAAATGCTAGAAAATGAGAGACCCGACAAGAAGAAAAGAGGAAGACCTACTACAAGGAACACACAGAAAGGGAAAGCAGGGGTCGAACAAGTTCGAAGAACTCGGGCACGTATAGCCAAGGGGTCAGGTAAAATAGGCGATGTTGAGTCAGACAGCAGTGACTCTACTGGTGAAAAACCAAAAGCGGAAGAAACTGGAATGGATAAAGGAAACATTGGAACAGTAAGCGAGGAAAACGCAAAAACAGATGAGTTCAATATGCTAGAAGACCGTGATTGGAAACAAAAAGGCAAGGCCATTGAACAAATTGATGTTGCAGAACCCAAATCTAATGTTCAGTTTGGTAAACTTCCAGAAGTCAATATATCTGAAAGGTATAATTACCAAGACACTGAAAAATCTGAAAAGCTGGAAGTCATGACCGATCCACTTCAAGCTATGTTATTAGATATGGTTCCAAGTCTTGGAATGAGTGAAACTAAAAGTAGTAGTACTGTTCTTGAGGAGAAGCAACCACCAGTACACAACAATTCTGAGCCAGCTAAGAAGAAAGTCAGCTACAAGGACGTTGCCAGTGAACTGCTTAAGGATTGTTACAAGTTAGAAGGCCACCTTTCCAGCAAAAAGCCATGTCCTCCAACAAAAATTTCAGCATCAAATGTAGCTGAAAATTCAGGAGCCTCAGCAGAAGCAGATGTCTTTGGAGTATCAAGTTCAAGACCTGCAGTGACAGCAGTAGTTAACCCACTTTATGAATCCTGGATAGCTATTGATCAGCTTCTTCTTAGCTGGCTATATAATTAA

Coding sequence (CDS)

ATGGATTCTGAGACACCATGGGCCTCGAAACATGTCTCGAAACATATAGAAAACCAGAAAATTTCCTCTCCTTCCAAGGTGTATAAAGATCGAGGAAGAAACGATGCTGCTTCAGGACAACACATTCTCTCATATTCAACTGAGAGATCTAGTGGAAACAAAAGAGGAAGGCCTGCTGCAAGGAGTATGCAGAAAGGGAAATCGGGTGTCGAACAAGTTCGAAGAGCTCGGGCACGAACAGCCAAGGGGCCTGCAAAAATAAGTGAAGTTGATTCAGACATCAGTGACCATACTGATGAAAAAACGAATGCAGAAACTGGAAATATTAGAACACTAAACAGTGAAAACTCAGAAAAGGAGTTTAAAATGCTAGAAAATGAGAGACCCGACAAGAAGAAAAGAGGAAGACCTACTACAAGGAACACACAGAAAGGGAAAGCAGGGGTCGAACAAGTTCGAAGAACTCGGGCACGTATAGCCAAGGGGTCAGGTAAAATAGGCGATGTTGAGTCAGACAGCAGTGACTCTACTGGTGAAAAACCAAAAGCGGAAGAAACTGGAATGGATAAAGGAAACATTGGAACAGTAAGCGAGGAAAACGCAAAAACAGATGAGTTCAATATGCTAGAAGACCGTGATTGGAAACAAAAAGGCAAGGCCATTGAACAAATTGATGTTGCAGAACCCAAATCTAATGTTCAGTTTGGTAAACTTCCAGAAGTCAATATATCTGAAAGGTATAATTACCAAGACACTGAAAAATCTGAAAAGCTGGAAGTCATGACCGATCCACTTCAAGCTATGTTATTAGATATGGTTCCAAGTCTTGGAATGAGTGAAACTAAAAGTAGTAGTACTGTTCTTGAGGAGAAGCAACCACCAGTACACAACAATTCTGAGCCAGCTAAGAAGAAAGTCAGCTACAAGGACGTTGCCAGTGAACTGCTTAAGGATTGTTACAAGTTAGAAGGCCACCTTTCCAGCAAAAAGCCATGTCCTCCAACAAAAATTTCAGCATCAAATGTAGCTGAAAATTCAGGAGCCTCAGCAGAAGCAGATGTCTTTGGAGTATCAAGTTCAAGACCTGCAGTGACAGCAGTAGTTAACCCACTTTATGAATCCTGGATAGCTATTGATCAGCTTCTTCTTAGCTGGCTATATAATTAA

Protein sequence

MDSETPWASKHVSKHIENQKISSPSKVYKDRGRNDAASGQHILSYSTERSSGNKRGRPAARSMQKGKSGVEQVRRARARTAKGPAKISEVDSDISDHTDEKTNAETGNIRTLNSENSEKEFKMLENERPDKKKRGRPTTRNTQKGKAGVEQVRRTRARIAKGSGKIGDVESDSSDSTGEKPKAEETGMDKGNIGTVSEENAKTDEFNMLEDRDWKQKGKAIEQIDVAEPKSNVQFGKLPEVNISERYNYQDTEKSEKLEVMTDPLQAMLLDMVPSLGMSETKSSSTVLEEKQPPVHNNSEPAKKKVSYKDVASELLKDCYKLEGHLSSKKPCPPTKISASNVAENSGASAEADVFGVSSSRPAVTAVVNPLYESWIAIDQLLLSWLYN
Homology
BLAST of Lag0006232 vs. NCBI nr
Match: XP_038889084.1 (DNA ligase 4 isoform X1 [Benincasa hispida])

HSP 1 Score: 372.1 bits (954), Expect = 5.9e-99
Identity = 224/319 (70.22%), Postives = 255/319 (79.94%), Query Frame = 0

Query: 1    MDSETPWASKHVSKHIENQKISSPSKVYKDRGRNDAASGQHILSYSTERSSGNKRGRPAA 60
            MD E P A    S++I+NQ+I SPS+ YKD+GRN+AASGQH LSYSTERS G KR RPAA
Sbjct: 923  MDFEMPSA----SENIDNQQIPSPSQEYKDQGRNEAASGQHTLSYSTERSGGKKRRRPAA 982

Query: 61   RSMQKGKSGVEQVRRARARTAKGPAKISEVDSDISDHTDEKTNAETGNIRTLNSENSEK- 120
            RSMQKGKSGV+QVR  R RTAKGPAKISEV+SD SD+TDEKTNAET N+ TL SE SEK 
Sbjct: 983  RSMQKGKSGVKQVREFRTRTAKGPAKISEVESDNSDYTDEKTNAETRNVGTLCSETSEKN 1042

Query: 121  EFKMLENERPDKKKRGRPTTRNTQKGKAGVEQVRRTRARIAKGSGKIGDVESDSSDSTGE 180
            EFK LENE  + KKRGRPT  NTQKGKAG + VRRTRARI KGS KIGD+ES S+DST E
Sbjct: 1043 EFKTLENEISNTKKRGRPTAGNTQKGKAGAKAVRRTRARITKGSAKIGDIESGSNDSTDE 1102

Query: 181  KPKAEETGMDKGNIGTVSEENAKTDEFNMLEDRDWKQKGKAIEQIDVAEPKSNVQFGKLP 240
            K   +E   +K       EEN+K DEF ML D D KQKGKAIE+   A+  SNV FGK+P
Sbjct: 1103 KTNTDELRKEK-------EENSKRDEFKMLIDHDPKQKGKAIEENVRADFGSNVDFGKVP 1162

Query: 241  EVNISERYNYQDTEKSEKLEVMTDPLQAMLLDMVPSLGMSETKSSSTVLEEKQPPVHNNS 300
            EV ISERY+++D EK EKLE+M DPL+AMLLDMVPSLGMS +KSSSTV+EEK+ PV++NS
Sbjct: 1163 EVGISERYDFKDVEKCEKLEIMKDPLEAMLLDMVPSLGMSGSKSSSTVVEEKK-PVNSNS 1222

Query: 301  EPAKKKVSYKDVASELLKD 319
            EPAKKKVSYKDVASELLKD
Sbjct: 1223 EPAKKKVSYKDVASELLKD 1229

BLAST of Lag0006232 vs. NCBI nr
Match: XP_038889088.1 (DNA ligase 4 isoform X4 [Benincasa hispida] >XP_038889089.1 DNA ligase 4 isoform X4 [Benincasa hispida] >XP_038889090.1 DNA ligase 4 isoform X4 [Benincasa hispida])

HSP 1 Score: 372.1 bits (954), Expect = 5.9e-99
Identity = 224/319 (70.22%), Postives = 255/319 (79.94%), Query Frame = 0

Query: 1    MDSETPWASKHVSKHIENQKISSPSKVYKDRGRNDAASGQHILSYSTERSSGNKRGRPAA 60
            MD E P A    S++I+NQ+I SPS+ YKD+GRN+AASGQH LSYSTERS G KR RPAA
Sbjct: 763  MDFEMPSA----SENIDNQQIPSPSQEYKDQGRNEAASGQHTLSYSTERSGGKKRRRPAA 822

Query: 61   RSMQKGKSGVEQVRRARARTAKGPAKISEVDSDISDHTDEKTNAETGNIRTLNSENSEK- 120
            RSMQKGKSGV+QVR  R RTAKGPAKISEV+SD SD+TDEKTNAET N+ TL SE SEK 
Sbjct: 823  RSMQKGKSGVKQVREFRTRTAKGPAKISEVESDNSDYTDEKTNAETRNVGTLCSETSEKN 882

Query: 121  EFKMLENERPDKKKRGRPTTRNTQKGKAGVEQVRRTRARIAKGSGKIGDVESDSSDSTGE 180
            EFK LENE  + KKRGRPT  NTQKGKAG + VRRTRARI KGS KIGD+ES S+DST E
Sbjct: 883  EFKTLENEISNTKKRGRPTAGNTQKGKAGAKAVRRTRARITKGSAKIGDIESGSNDSTDE 942

Query: 181  KPKAEETGMDKGNIGTVSEENAKTDEFNMLEDRDWKQKGKAIEQIDVAEPKSNVQFGKLP 240
            K   +E   +K       EEN+K DEF ML D D KQKGKAIE+   A+  SNV FGK+P
Sbjct: 943  KTNTDELRKEK-------EENSKRDEFKMLIDHDPKQKGKAIEENVRADFGSNVDFGKVP 1002

Query: 241  EVNISERYNYQDTEKSEKLEVMTDPLQAMLLDMVPSLGMSETKSSSTVLEEKQPPVHNNS 300
            EV ISERY+++D EK EKLE+M DPL+AMLLDMVPSLGMS +KSSSTV+EEK+ PV++NS
Sbjct: 1003 EVGISERYDFKDVEKCEKLEIMKDPLEAMLLDMVPSLGMSGSKSSSTVVEEKK-PVNSNS 1062

Query: 301  EPAKKKVSYKDVASELLKD 319
            EPAKKKVSYKDVASELLKD
Sbjct: 1063 EPAKKKVSYKDVASELLKD 1069

BLAST of Lag0006232 vs. NCBI nr
Match: XP_038889086.1 (DNA ligase 4 isoform X3 [Benincasa hispida] >XP_038889087.1 DNA ligase 4 isoform X3 [Benincasa hispida])

HSP 1 Score: 372.1 bits (954), Expect = 5.9e-99
Identity = 224/319 (70.22%), Postives = 255/319 (79.94%), Query Frame = 0

Query: 1    MDSETPWASKHVSKHIENQKISSPSKVYKDRGRNDAASGQHILSYSTERSSGNKRGRPAA 60
            MD E P A    S++I+NQ+I SPS+ YKD+GRN+AASGQH LSYSTERS G KR RPAA
Sbjct: 805  MDFEMPSA----SENIDNQQIPSPSQEYKDQGRNEAASGQHTLSYSTERSGGKKRRRPAA 864

Query: 61   RSMQKGKSGVEQVRRARARTAKGPAKISEVDSDISDHTDEKTNAETGNIRTLNSENSEK- 120
            RSMQKGKSGV+QVR  R RTAKGPAKISEV+SD SD+TDEKTNAET N+ TL SE SEK 
Sbjct: 865  RSMQKGKSGVKQVREFRTRTAKGPAKISEVESDNSDYTDEKTNAETRNVGTLCSETSEKN 924

Query: 121  EFKMLENERPDKKKRGRPTTRNTQKGKAGVEQVRRTRARIAKGSGKIGDVESDSSDSTGE 180
            EFK LENE  + KKRGRPT  NTQKGKAG + VRRTRARI KGS KIGD+ES S+DST E
Sbjct: 925  EFKTLENEISNTKKRGRPTAGNTQKGKAGAKAVRRTRARITKGSAKIGDIESGSNDSTDE 984

Query: 181  KPKAEETGMDKGNIGTVSEENAKTDEFNMLEDRDWKQKGKAIEQIDVAEPKSNVQFGKLP 240
            K   +E   +K       EEN+K DEF ML D D KQKGKAIE+   A+  SNV FGK+P
Sbjct: 985  KTNTDELRKEK-------EENSKRDEFKMLIDHDPKQKGKAIEENVRADFGSNVDFGKVP 1044

Query: 241  EVNISERYNYQDTEKSEKLEVMTDPLQAMLLDMVPSLGMSETKSSSTVLEEKQPPVHNNS 300
            EV ISERY+++D EK EKLE+M DPL+AMLLDMVPSLGMS +KSSSTV+EEK+ PV++NS
Sbjct: 1045 EVGISERYDFKDVEKCEKLEIMKDPLEAMLLDMVPSLGMSGSKSSSTVVEEKK-PVNSNS 1104

Query: 301  EPAKKKVSYKDVASELLKD 319
            EPAKKKVSYKDVASELLKD
Sbjct: 1105 EPAKKKVSYKDVASELLKD 1111

BLAST of Lag0006232 vs. NCBI nr
Match: XP_038889085.1 (DNA ligase 4 isoform X2 [Benincasa hispida])

HSP 1 Score: 372.1 bits (954), Expect = 5.9e-99
Identity = 224/319 (70.22%), Postives = 255/319 (79.94%), Query Frame = 0

Query: 1    MDSETPWASKHVSKHIENQKISSPSKVYKDRGRNDAASGQHILSYSTERSSGNKRGRPAA 60
            MD E P A    S++I+NQ+I SPS+ YKD+GRN+AASGQH LSYSTERS G KR RPAA
Sbjct: 891  MDFEMPSA----SENIDNQQIPSPSQEYKDQGRNEAASGQHTLSYSTERSGGKKRRRPAA 950

Query: 61   RSMQKGKSGVEQVRRARARTAKGPAKISEVDSDISDHTDEKTNAETGNIRTLNSENSEK- 120
            RSMQKGKSGV+QVR  R RTAKGPAKISEV+SD SD+TDEKTNAET N+ TL SE SEK 
Sbjct: 951  RSMQKGKSGVKQVREFRTRTAKGPAKISEVESDNSDYTDEKTNAETRNVGTLCSETSEKN 1010

Query: 121  EFKMLENERPDKKKRGRPTTRNTQKGKAGVEQVRRTRARIAKGSGKIGDVESDSSDSTGE 180
            EFK LENE  + KKRGRPT  NTQKGKAG + VRRTRARI KGS KIGD+ES S+DST E
Sbjct: 1011 EFKTLENEISNTKKRGRPTAGNTQKGKAGAKAVRRTRARITKGSAKIGDIESGSNDSTDE 1070

Query: 181  KPKAEETGMDKGNIGTVSEENAKTDEFNMLEDRDWKQKGKAIEQIDVAEPKSNVQFGKLP 240
            K   +E   +K       EEN+K DEF ML D D KQKGKAIE+   A+  SNV FGK+P
Sbjct: 1071 KTNTDELRKEK-------EENSKRDEFKMLIDHDPKQKGKAIEENVRADFGSNVDFGKVP 1130

Query: 241  EVNISERYNYQDTEKSEKLEVMTDPLQAMLLDMVPSLGMSETKSSSTVLEEKQPPVHNNS 300
            EV ISERY+++D EK EKLE+M DPL+AMLLDMVPSLGMS +KSSSTV+EEK+ PV++NS
Sbjct: 1131 EVGISERYDFKDVEKCEKLEIMKDPLEAMLLDMVPSLGMSGSKSSSTVVEEKK-PVNSNS 1190

Query: 301  EPAKKKVSYKDVASELLKD 319
            EPAKKKVSYKDVASELLKD
Sbjct: 1191 EPAKKKVSYKDVASELLKD 1197

BLAST of Lag0006232 vs. NCBI nr
Match: XP_022149864.1 (DNA ligase 4 isoform X3 [Momordica charantia])

HSP 1 Score: 349.7 bits (896), Expect = 3.1e-92
Identity = 226/342 (66.08%), Postives = 249/342 (72.81%), Query Frame = 0

Query: 1    MDSETPWASKHVSKHIENQKISSPSKVYKDRGRNDAASGQHILSYSTERSSGNKRGRPAA 60
            +DSETP AS++     ENQKISSPS  YKD+GRN+AASGQH+L +STERS G KRG+  A
Sbjct: 924  IDSETPLASENT----ENQKISSPSNAYKDQGRNEAASGQHMLLHSTERSGGKKRGKSTA 983

Query: 61   RSMQKGKSGVEQVRRARARTAKGPAKISEVDSDISDHTDEKTNAETGNIRTLNSEN-SEK 120
            RSMQKGK  VEQVRRARARTAKGPAKISEV+SD SD+ DEKTNAET NI T  SEN    
Sbjct: 984  RSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYNDEKTNAETENIGTPCSENLKTN 1043

Query: 121  EFKMLEN------------------ERPDKKKRGRPTT-----RNTQKGKAGVEQVRRTR 180
            E   LEN                  ER  +KKRGR  T     R+ QKGKAGVEQ +RTR
Sbjct: 1044 ELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPTPTASARSMQKGKAGVEQGQRTR 1103

Query: 181  ARIAKGSGKIGDVESDSSDSTGEKPKAEETGMDKGNIGTVSEENAKTDEFNMLEDRDWKQ 240
            AR AKGS KIG VESDSSD+T +K  AE  G++K NIGTV   N+ T+EF   E+ D   
Sbjct: 1104 ARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNIGTVCPANSNTNEFIWPENCDSSH 1163

Query: 241  KGKAIEQIDVAEPKSNVQFGKLPEVNISERYNYQDTEKSEKLEVMTDPLQAMLLDMVPSL 300
            KGK IEQ D+AE KSNV     PE+ IS RYN Q  E+SEKLEVMTDPLQAMLLDMVPSL
Sbjct: 1164 KGKVIEQDDLAEFKSNV-----PEIEISTRYNCQGIEESEKLEVMTDPLQAMLLDMVPSL 1223

Query: 301  GMSETKSSSTVLEEKQPPVHNNSEPAKKKVSYKDVASELLKD 319
            GMSETKSSSTVLEEKQ PV NNSEP KKKVSYKDVASELLKD
Sbjct: 1224 GMSETKSSSTVLEEKQ-PVQNNSEPTKKKVSYKDVASELLKD 1255

BLAST of Lag0006232 vs. ExPASy Swiss-Prot
Match: Q9LL84 (DNA ligase 4 OS=Arabidopsis thaliana OX=3702 GN=LIG4 PE=1 SV=1)

HSP 1 Score: 58.9 bits (141), Expect = 1.4e-07
Identity = 76/296 (25.68%), Postives = 129/296 (43.58%), Query Frame = 0

Query: 53   NKRGRPAARSMQKGKSGVEQVRRARARTAKGPAKISEVDSDISDHTDEKTNAETGNIRTL 112
            + RGR   R++++G+S    ++R + R  K P+KIS  +++ SD ++EK +    +I   
Sbjct: 952  SSRGRSNTRAVKRGRSSTNSLQRVQRRRGKQPSKISGDETEESDASEEKVSTRLSDIAE- 1011

Query: 113  NSENSEKEFKMLENERPDKKKRGRPTTRNTQKGKAGVEQVRRT-RARIAKGSGKIGDVES 172
                        E +   + +R     +  ++GK+ V Q +R  R+R  K + KIG  ES
Sbjct: 1012 ------------ETDSFGEAQRNSSRGKCAKRGKSRVGQTQRVQRSRRGKKAAKIGGDES 1071

Query: 173  DSSDSTGEKPKAEETGMDKGNIGTVSEENAKTDEFNMLEDRDWKQKGKAIEQIDVAEPKS 232
            D +D             ++GN    S EN +T E ++ +  + +Q+   +   +  +   
Sbjct: 1072 DENDELDGNNNV-SADAEEGNAAGRSVENEETREPDIAKYTESQQRDNTVAVEEALQDSR 1131

Query: 233  NVQFGKLPEVNISERYNYQDTEKSEKLEVMTDPLQAMLLDMVPSLGMSETKSSS-TVLEE 292
            N +                + +  EKL++  DPLQAML+ M P      T++S+ T  E 
Sbjct: 1132 NAK---------------TEMDMKEKLQIHEDPLQAMLMKMFPIPSQKTTETSNRTTGEY 1191

Query: 293  KQPPVHNNSEPA----------------------------KKKVSYKDVASELLKD 319
            ++  V    E +                            KKKVSY+DVA ELLKD
Sbjct: 1192 RKANVSGECESSEKRKLDAETDNTSVNAGAESDVVPPLVKKKKVSYRDVAGELLKD 1218

BLAST of Lag0006232 vs. ExPASy Swiss-Prot
Match: Q7X7E9 (Putative DNA ligase 4 OS=Oryza sativa subsp. japonica OX=39947 GN=LIG4 PE=2 SV=2)

HSP 1 Score: 52.4 bits (124), Expect = 1.4e-05
Identity = 97/376 (25.80%), Postives = 146/376 (38.83%), Query Frame = 0

Query: 34   NDAASGQHILSYSTERSSGNKRGRPAARSMQKGKSGVEQVRRARARTAKGPAKISEVDSD 93
            N+ A   H+      + +  KRGRP++ + +  K     VRR RAR     AKI +V+ +
Sbjct: 962  NEKADTSHV------KHAPRKRGRPSSSASRTAKPAPRPVRRTRARRGNQHAKIDDVEPE 1021

Query: 94   ISDHTD---EKTNAETGNIRTLNSENSEKEFKMLENERPDKKKRGRP-------TTRNTQ 153
             SDH +   +    +T NI  +  ++ +K+       R  + +RG+         +  + 
Sbjct: 1022 ESDHGETGLDDQIPDTDNISKMEVDSFDKDQVSARPVRRTRARRGKQHAKIDYGQSEESD 1081

Query: 154  KGKAGVEQVRRTRARIAKGSGKIGDVESDSSD-STGEKPKAE------------------ 213
             G+ G +  R     I+K       +E DSSD   G  P A                   
Sbjct: 1082 PGETGQDDQRLDADYISK-------MEEDSSDRDQGAHPTAPRVVRRSRAQRGKWLAKID 1141

Query: 214  ------ETGMDKGNIGTVSEENAKTDEFNMLEDRDWKQKGKAIEQIDVAEPK----SNVQ 273
                  ETG D   +   ++  +K +E    +D++    G  +  +D  EPK    S  +
Sbjct: 1142 RETGPGETGQDDKKLN--ADSISKMEEHAHDKDQE-PPPGAQLITLDEQEPKGIKSSTTE 1201

Query: 274  FGKLPEVNISERYNYQDTEKS------EKLEVMTDPLQAMLLDMVPSLGMSETKSSSTVL 319
                P+   ++    +DT ++      EK+E M DPL AMLLDM+PSLG  +T   + V 
Sbjct: 1202 TPSSPKHERNQTVLRRDTAETTSSATCEKMEQMVDPLHAMLLDMIPSLGQMKTDVGNRVA 1261

BLAST of Lag0006232 vs. ExPASy TrEMBL
Match: A0A6J1D962 (DNA ligase OS=Momordica charantia OX=3673 GN=LOC111018188 PE=3 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 1.5e-92
Identity = 226/342 (66.08%), Postives = 249/342 (72.81%), Query Frame = 0

Query: 1    MDSETPWASKHVSKHIENQKISSPSKVYKDRGRNDAASGQHILSYSTERSSGNKRGRPAA 60
            +DSETP AS++     ENQKISSPS  YKD+GRN+AASGQH+L +STERS G KRG+  A
Sbjct: 833  IDSETPLASENT----ENQKISSPSNAYKDQGRNEAASGQHMLLHSTERSGGKKRGKSTA 892

Query: 61   RSMQKGKSGVEQVRRARARTAKGPAKISEVDSDISDHTDEKTNAETGNIRTLNSEN-SEK 120
            RSMQKGK  VEQVRRARARTAKGPAKISEV+SD SD+ DEKTNAET NI T  SEN    
Sbjct: 893  RSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYNDEKTNAETENIGTPCSENLKTN 952

Query: 121  EFKMLEN------------------ERPDKKKRGRPTT-----RNTQKGKAGVEQVRRTR 180
            E   LEN                  ER  +KKRGR  T     R+ QKGKAGVEQ +RTR
Sbjct: 953  ELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPTPTASARSMQKGKAGVEQGQRTR 1012

Query: 181  ARIAKGSGKIGDVESDSSDSTGEKPKAEETGMDKGNIGTVSEENAKTDEFNMLEDRDWKQ 240
            AR AKGS KIG VESDSSD+T +K  AE  G++K NIGTV   N+ T+EF   E+ D   
Sbjct: 1013 ARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNIGTVCPANSNTNEFIWPENCDSSH 1072

Query: 241  KGKAIEQIDVAEPKSNVQFGKLPEVNISERYNYQDTEKSEKLEVMTDPLQAMLLDMVPSL 300
            KGK IEQ D+AE KSNV     PE+ IS RYN Q  E+SEKLEVMTDPLQAMLLDMVPSL
Sbjct: 1073 KGKVIEQDDLAEFKSNV-----PEIEISTRYNCQGIEESEKLEVMTDPLQAMLLDMVPSL 1132

Query: 301  GMSETKSSSTVLEEKQPPVHNNSEPAKKKVSYKDVASELLKD 319
            GMSETKSSSTVLEEKQ PV NNSEP KKKVSYKDVASELLKD
Sbjct: 1133 GMSETKSSSTVLEEKQ-PVQNNSEPTKKKVSYKDVASELLKD 1164

BLAST of Lag0006232 vs. ExPASy TrEMBL
Match: A0A6J1D6X8 (DNA ligase OS=Momordica charantia OX=3673 GN=LOC111018188 PE=3 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 1.5e-92
Identity = 226/342 (66.08%), Postives = 249/342 (72.81%), Query Frame = 0

Query: 1    MDSETPWASKHVSKHIENQKISSPSKVYKDRGRNDAASGQHILSYSTERSSGNKRGRPAA 60
            +DSETP AS++     ENQKISSPS  YKD+GRN+AASGQH+L +STERS G KRG+  A
Sbjct: 923  IDSETPLASENT----ENQKISSPSNAYKDQGRNEAASGQHMLLHSTERSGGKKRGKSTA 982

Query: 61   RSMQKGKSGVEQVRRARARTAKGPAKISEVDSDISDHTDEKTNAETGNIRTLNSEN-SEK 120
            RSMQKGK  VEQVRRARARTAKGPAKISEV+SD SD+ DEKTNAET NI T  SEN    
Sbjct: 983  RSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYNDEKTNAETENIGTPCSENLKTN 1042

Query: 121  EFKMLEN------------------ERPDKKKRGRPTT-----RNTQKGKAGVEQVRRTR 180
            E   LEN                  ER  +KKRGR  T     R+ QKGKAGVEQ +RTR
Sbjct: 1043 ELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPTPTASARSMQKGKAGVEQGQRTR 1102

Query: 181  ARIAKGSGKIGDVESDSSDSTGEKPKAEETGMDKGNIGTVSEENAKTDEFNMLEDRDWKQ 240
            AR AKGS KIG VESDSSD+T +K  AE  G++K NIGTV   N+ T+EF   E+ D   
Sbjct: 1103 ARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNIGTVCPANSNTNEFIWPENCDSSH 1162

Query: 241  KGKAIEQIDVAEPKSNVQFGKLPEVNISERYNYQDTEKSEKLEVMTDPLQAMLLDMVPSL 300
            KGK IEQ D+AE KSNV     PE+ IS RYN Q  E+SEKLEVMTDPLQAMLLDMVPSL
Sbjct: 1163 KGKVIEQDDLAEFKSNV-----PEIEISTRYNCQGIEESEKLEVMTDPLQAMLLDMVPSL 1222

Query: 301  GMSETKSSSTVLEEKQPPVHNNSEPAKKKVSYKDVASELLKD 319
            GMSETKSSSTVLEEKQ PV NNSEP KKKVSYKDVASELLKD
Sbjct: 1223 GMSETKSSSTVLEEKQ-PVQNNSEPTKKKVSYKDVASELLKD 1254

BLAST of Lag0006232 vs. ExPASy TrEMBL
Match: A0A6J1D7W8 (DNA ligase OS=Momordica charantia OX=3673 GN=LOC111018188 PE=3 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 1.5e-92
Identity = 226/342 (66.08%), Postives = 249/342 (72.81%), Query Frame = 0

Query: 1    MDSETPWASKHVSKHIENQKISSPSKVYKDRGRNDAASGQHILSYSTERSSGNKRGRPAA 60
            +DSETP AS++     ENQKISSPS  YKD+GRN+AASGQH+L +STERS G KRG+  A
Sbjct: 924  IDSETPLASENT----ENQKISSPSNAYKDQGRNEAASGQHMLLHSTERSGGKKRGKSTA 983

Query: 61   RSMQKGKSGVEQVRRARARTAKGPAKISEVDSDISDHTDEKTNAETGNIRTLNSEN-SEK 120
            RSMQKGK  VEQVRRARARTAKGPAKISEV+SD SD+ DEKTNAET NI T  SEN    
Sbjct: 984  RSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYNDEKTNAETENIGTPCSENLKTN 1043

Query: 121  EFKMLEN------------------ERPDKKKRGRPTT-----RNTQKGKAGVEQVRRTR 180
            E   LEN                  ER  +KKRGR  T     R+ QKGKAGVEQ +RTR
Sbjct: 1044 ELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPTPTASARSMQKGKAGVEQGQRTR 1103

Query: 181  ARIAKGSGKIGDVESDSSDSTGEKPKAEETGMDKGNIGTVSEENAKTDEFNMLEDRDWKQ 240
            AR AKGS KIG VESDSSD+T +K  AE  G++K NIGTV   N+ T+EF   E+ D   
Sbjct: 1104 ARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNIGTVCPANSNTNEFIWPENCDSSH 1163

Query: 241  KGKAIEQIDVAEPKSNVQFGKLPEVNISERYNYQDTEKSEKLEVMTDPLQAMLLDMVPSL 300
            KGK IEQ D+AE KSNV     PE+ IS RYN Q  E+SEKLEVMTDPLQAMLLDMVPSL
Sbjct: 1164 KGKVIEQDDLAEFKSNV-----PEIEISTRYNCQGIEESEKLEVMTDPLQAMLLDMVPSL 1223

Query: 301  GMSETKSSSTVLEEKQPPVHNNSEPAKKKVSYKDVASELLKD 319
            GMSETKSSSTVLEEKQ PV NNSEP KKKVSYKDVASELLKD
Sbjct: 1224 GMSETKSSSTVLEEKQ-PVQNNSEPTKKKVSYKDVASELLKD 1255

BLAST of Lag0006232 vs. ExPASy TrEMBL
Match: A0A6J1D8A6 (DNA ligase OS=Momordica charantia OX=3673 GN=LOC111018188 PE=3 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 1.5e-92
Identity = 226/342 (66.08%), Postives = 249/342 (72.81%), Query Frame = 0

Query: 1    MDSETPWASKHVSKHIENQKISSPSKVYKDRGRNDAASGQHILSYSTERSSGNKRGRPAA 60
            +DSETP AS++     ENQKISSPS  YKD+GRN+AASGQH+L +STERS G KRG+  A
Sbjct: 924  IDSETPLASENT----ENQKISSPSNAYKDQGRNEAASGQHMLLHSTERSGGKKRGKSTA 983

Query: 61   RSMQKGKSGVEQVRRARARTAKGPAKISEVDSDISDHTDEKTNAETGNIRTLNSEN-SEK 120
            RSMQKGK  VEQVRRARARTAKGPAKISEV+SD SD+ DEKTNAET NI T  SEN    
Sbjct: 984  RSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYNDEKTNAETENIGTPCSENLKTN 1043

Query: 121  EFKMLEN------------------ERPDKKKRGRPTT-----RNTQKGKAGVEQVRRTR 180
            E   LEN                  ER  +KKRGR  T     R+ QKGKAGVEQ +RTR
Sbjct: 1044 ELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPTPTASARSMQKGKAGVEQGQRTR 1103

Query: 181  ARIAKGSGKIGDVESDSSDSTGEKPKAEETGMDKGNIGTVSEENAKTDEFNMLEDRDWKQ 240
            AR AKGS KIG VESDSSD+T +K  AE  G++K NIGTV   N+ T+EF   E+ D   
Sbjct: 1104 ARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNIGTVCPANSNTNEFIWPENCDSSH 1163

Query: 241  KGKAIEQIDVAEPKSNVQFGKLPEVNISERYNYQDTEKSEKLEVMTDPLQAMLLDMVPSL 300
            KGK IEQ D+AE KSNV     PE+ IS RYN Q  E+SEKLEVMTDPLQAMLLDMVPSL
Sbjct: 1164 KGKVIEQDDLAEFKSNV-----PEIEISTRYNCQGIEESEKLEVMTDPLQAMLLDMVPSL 1223

Query: 301  GMSETKSSSTVLEEKQPPVHNNSEPAKKKVSYKDVASELLKD 319
            GMSETKSSSTVLEEKQ PV NNSEP KKKVSYKDVASELLKD
Sbjct: 1224 GMSETKSSSTVLEEKQ-PVQNNSEPTKKKVSYKDVASELLKD 1255

BLAST of Lag0006232 vs. ExPASy TrEMBL
Match: A0A6J1D9Q4 (DNA ligase IV OS=Momordica charantia OX=3673 GN=LOC111018188 PE=3 SV=1)

HSP 1 Score: 349.7 bits (896), Expect = 1.5e-92
Identity = 226/342 (66.08%), Postives = 249/342 (72.81%), Query Frame = 0

Query: 1    MDSETPWASKHVSKHIENQKISSPSKVYKDRGRNDAASGQHILSYSTERSSGNKRGRPAA 60
            +DSETP AS++     ENQKISSPS  YKD+GRN+AASGQH+L +STERS G KRG+  A
Sbjct: 901  IDSETPLASENT----ENQKISSPSNAYKDQGRNEAASGQHMLLHSTERSGGKKRGKSTA 960

Query: 61   RSMQKGKSGVEQVRRARARTAKGPAKISEVDSDISDHTDEKTNAETGNIRTLNSEN-SEK 120
            RSMQKGK  VEQVRRARARTAKGPAKISEV+SD SD+ DEKTNAET NI T  SEN    
Sbjct: 961  RSMQKGKLSVEQVRRARARTAKGPAKISEVESDSSDYNDEKTNAETENIGTPCSENLKTN 1020

Query: 121  EFKMLEN------------------ERPDKKKRGRPTT-----RNTQKGKAGVEQVRRTR 180
            E   LEN                  ER  +KKRGR  T     R+ QKGKAGVEQ +RTR
Sbjct: 1021 ELTKLENFDPSRKGKGIEHVPLCSTERTGEKKRGRRPTPTASARSMQKGKAGVEQGQRTR 1080

Query: 181  ARIAKGSGKIGDVESDSSDSTGEKPKAEETGMDKGNIGTVSEENAKTDEFNMLEDRDWKQ 240
            AR AKGS KIG VESDSSD+T +K  AE  G++K NIGTV   N+ T+EF   E+ D   
Sbjct: 1081 ARTAKGSAKIGGVESDSSDTTDDKTNAEGNGVEKRNIGTVCPANSNTNEFIWPENCDSSH 1140

Query: 241  KGKAIEQIDVAEPKSNVQFGKLPEVNISERYNYQDTEKSEKLEVMTDPLQAMLLDMVPSL 300
            KGK IEQ D+AE KSNV     PE+ IS RYN Q  E+SEKLEVMTDPLQAMLLDMVPSL
Sbjct: 1141 KGKVIEQDDLAEFKSNV-----PEIEISTRYNCQGIEESEKLEVMTDPLQAMLLDMVPSL 1200

Query: 301  GMSETKSSSTVLEEKQPPVHNNSEPAKKKVSYKDVASELLKD 319
            GMSETKSSSTVLEEKQ PV NNSEP KKKVSYKDVASELLKD
Sbjct: 1201 GMSETKSSSTVLEEKQ-PVQNNSEPTKKKVSYKDVASELLKD 1232

BLAST of Lag0006232 vs. TAIR 10
Match: AT5G57160.1 (DNA ligase IV )

HSP 1 Score: 58.9 bits (141), Expect = 1.0e-08
Identity = 76/296 (25.68%), Postives = 129/296 (43.58%), Query Frame = 0

Query: 53   NKRGRPAARSMQKGKSGVEQVRRARARTAKGPAKISEVDSDISDHTDEKTNAETGNIRTL 112
            + RGR   R++++G+S    ++R + R  K P+KIS  +++ SD ++EK +    +I   
Sbjct: 952  SSRGRSNTRAVKRGRSSTNSLQRVQRRRGKQPSKISGDETEESDASEEKVSTRLSDIAE- 1011

Query: 113  NSENSEKEFKMLENERPDKKKRGRPTTRNTQKGKAGVEQVRRT-RARIAKGSGKIGDVES 172
                        E +   + +R     +  ++GK+ V Q +R  R+R  K + KIG  ES
Sbjct: 1012 ------------ETDSFGEAQRNSSRGKCAKRGKSRVGQTQRVQRSRRGKKAAKIGGDES 1071

Query: 173  DSSDSTGEKPKAEETGMDKGNIGTVSEENAKTDEFNMLEDRDWKQKGKAIEQIDVAEPKS 232
            D +D             ++GN    S EN +T E ++ +  + +Q+   +   +  +   
Sbjct: 1072 DENDELDGNNNV-SADAEEGNAAGRSVENEETREPDIAKYTESQQRDNTVAVEEALQDSR 1131

Query: 233  NVQFGKLPEVNISERYNYQDTEKSEKLEVMTDPLQAMLLDMVPSLGMSETKSSS-TVLEE 292
            N +                + +  EKL++  DPLQAML+ M P      T++S+ T  E 
Sbjct: 1132 NAK---------------TEMDMKEKLQIHEDPLQAMLMKMFPIPSQKTTETSNRTTGEY 1191

Query: 293  KQPPVHNNSEPA----------------------------KKKVSYKDVASELLKD 319
            ++  V    E +                            KKKVSY+DVA ELLKD
Sbjct: 1192 RKANVSGECESSEKRKLDAETDNTSVNAGAESDVVPPLVKKKKVSYRDVAGELLKD 1218

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038889084.15.9e-9970.22DNA ligase 4 isoform X1 [Benincasa hispida][more]
XP_038889088.15.9e-9970.22DNA ligase 4 isoform X4 [Benincasa hispida] >XP_038889089.1 DNA ligase 4 isoform... [more]
XP_038889086.15.9e-9970.22DNA ligase 4 isoform X3 [Benincasa hispida] >XP_038889087.1 DNA ligase 4 isoform... [more]
XP_038889085.15.9e-9970.22DNA ligase 4 isoform X2 [Benincasa hispida][more]
XP_022149864.13.1e-9266.08DNA ligase 4 isoform X3 [Momordica charantia][more]
Match NameE-valueIdentityDescription
Q9LL841.4e-0725.68DNA ligase 4 OS=Arabidopsis thaliana OX=3702 GN=LIG4 PE=1 SV=1[more]
Q7X7E91.4e-0525.80Putative DNA ligase 4 OS=Oryza sativa subsp. japonica OX=39947 GN=LIG4 PE=2 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1D9621.5e-9266.08DNA ligase OS=Momordica charantia OX=3673 GN=LOC111018188 PE=3 SV=1[more]
A0A6J1D6X81.5e-9266.08DNA ligase OS=Momordica charantia OX=3673 GN=LOC111018188 PE=3 SV=1[more]
A0A6J1D7W81.5e-9266.08DNA ligase OS=Momordica charantia OX=3673 GN=LOC111018188 PE=3 SV=1[more]
A0A6J1D8A61.5e-9266.08DNA ligase OS=Momordica charantia OX=3673 GN=LOC111018188 PE=3 SV=1[more]
A0A6J1D9Q41.5e-9266.08DNA ligase IV OS=Momordica charantia OX=3673 GN=LOC111018188 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT5G57160.11.0e-0825.68DNA ligase IV [more]
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..200
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 68..104
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 115..136
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..17
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 39..53
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 280..304
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 147..165
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 172..187

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0006232.1Lag0006232.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0034645 cellular macromolecule biosynthetic process
biological_process GO:0006302 double-strand break repair
cellular_component GO:0005634 nucleus
molecular_function GO:0003909 DNA ligase activity
molecular_function GO:0000166 nucleotide binding