Lag0006195 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0006195
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRNase H domain-containing protein
Locationchr6: 38906331 .. 38906843 (+)
RNA-Seq ExpressionLag0006195
SyntenyLag0006195
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGATTTCAGGAGGGGAGGATATTATTTTGCATGTTGATGCAGCTTGGACCAAACACAACAAGACCGGTGGAGCAAAGGTAGTTCTGCGCACAAAGATGGGAAAGTTGGTGGCCATTATGCAAAAAGGGATTCCTTTACCCTCATCTCCATTATGTGTTGAGGAGATAGTGGTTCTTGAGGGTCTTAAAATGACTTCTATTAGGAATATTCGTAAGGTAATGGTATGCTCGGATTCCCTATCGTTGATCTCCATTCTTCGTAAAGATGATCGGTGTCATGCAGATTGCTTTCCCGTTGTGGCATATATTCAAAGTTTCTTTTCATCTTTTGAAAAGATTAACTTTTGTCATATAAATCGTGAGTATAACTTACTGTCGCATGAACTGGCTAGTTTAGGCATGGATTCCACTACTCGAGTTTGGAGTAGGAATTTCCTAGATTGGGCTTTAGATTTAGCTAGTAGAGAATTGTCTTGTTTTGTATCCCGCGAGGGAATCATTGTTCTTGAATGA

mRNA sequence

ATGATTTCAGGAGGGGAGGATATTATTTTGCATGTTGATGCAGCTTGGACCAAACACAACAAGACCGGTGGAGCAAAGGTAGTTCTGCGCACAAAGATGGGAAAGTTGGTGGCCATTATGCAAAAAGGGATTCCTTTACCCTCATCTCCATTATGTGTTGAGGAGATAGTGGTTCTTGAGGGTCTTAAAATGACTTCTATTAGGAATATTCGTAAGGTAATGGTATGCTCGGATTCCCTATCGTTGATCTCCATTCTTCGTAAAGATGATCGGTGTCATGCAGATTGCTTTCCCGTTGTGGCATATATTCAAAGTTTCTTTTCATCTTTTGAAAAGATTAACTTTTGTCATATAAATCGTGAGTATAACTTACTGTCGCATGAACTGGCTAGTTTAGGCATGGATTCCACTACTCGAGTTTGGAGTAGGAATTTCCTAGATTGGGCTTTAGATTTAGCTAGTAGAGAATTGTCTTGTTTTGTATCCCGCGAGGGAATCATTGTTCTTGAATGA

Coding sequence (CDS)

ATGATTTCAGGAGGGGAGGATATTATTTTGCATGTTGATGCAGCTTGGACCAAACACAACAAGACCGGTGGAGCAAAGGTAGTTCTGCGCACAAAGATGGGAAAGTTGGTGGCCATTATGCAAAAAGGGATTCCTTTACCCTCATCTCCATTATGTGTTGAGGAGATAGTGGTTCTTGAGGGTCTTAAAATGACTTCTATTAGGAATATTCGTAAGGTAATGGTATGCTCGGATTCCCTATCGTTGATCTCCATTCTTCGTAAAGATGATCGGTGTCATGCAGATTGCTTTCCCGTTGTGGCATATATTCAAAGTTTCTTTTCATCTTTTGAAAAGATTAACTTTTGTCATATAAATCGTGAGTATAACTTACTGTCGCATGAACTGGCTAGTTTAGGCATGGATTCCACTACTCGAGTTTGGAGTAGGAATTTCCTAGATTGGGCTTTAGATTTAGCTAGTAGAGAATTGTCTTGTTTTGTATCCCGCGAGGGAATCATTGTTCTTGAATGA

Protein sequence

MISGGEDIILHVDAAWTKHNKTGGAKVVLRTKMGKLVAIMQKGIPLPSSPLCVEEIVVLEGLKMTSIRNIRKVMVCSDSLSLISILRKDDRCHADCFPVVAYIQSFFSSFEKINFCHINREYNLLSHELASLGMDSTTRVWSRNFLDWALDLASRELSCFVSREGIIVLE
Homology
BLAST of Lag0006195 vs. NCBI nr
Match: XP_016199994.1 (uncharacterized protein LOC107641002 [Arachis ipaensis] >XP_025652971.1 uncharacterized protein LOC112748935 [Arachis hypogaea])

HSP 1 Score: 66.6 bits (161), Expect = 2.3e-07
Identity = 44/143 (30.77%), Postives = 72/143 (50.35%), Query Frame = 0

Query: 12  VDAAWTKHNKTGGAKVVLRTKMGKLVAIMQKGIPLPSSPLCVEEIVVLEGLKMTSIRNIR 71
           VDAA   H+  GG  VV+R +MG ++A+  + IP P      E      GL         
Sbjct: 28  VDAA-VPHDINGGVGVVIRDEMGIIMALAIQAIPYPFEAHEAEAYAAFWGLNFIKDCCFA 87

Query: 72  KVMVCSDSLSLISILRKDDRCHADCF-PVVAYIQSFFSSFEKINFCHINREYNLLSHELA 131
           KV++ SD+  ++  L++  RC + CF   +    S   +F  + F H+ RE N ++HELA
Sbjct: 88  KVILKSDNTEVMGALKQ--RCFSSCFGTFIIDAISLIKNFRSVEFSHVKREGNRVAHELA 147

Query: 132 SLGMDSTTRVWSRNFLDWALDLA 154
           +L + +   +W  + L+   +LA
Sbjct: 148 TLALTNPNCMWMEDVLNHIQNLA 167

BLAST of Lag0006195 vs. NCBI nr
Match: VAH31913.1 (unnamed protein product [Triticum turgidum subsp. durum])

HSP 1 Score: 65.1 bits (157), Expect = 6.8e-07
Identity = 42/144 (29.17%), Postives = 68/144 (47.22%), Query Frame = 0

Query: 5   GEDIILHVDAAWTKHNKTGGAKVVLRTKMGKLVAIMQKGIPLPSSPLCVEEIVVLEGLKM 64
           G+ + ++VDA++      G    V+R K GK +A     + +    L  E + +  GL +
Sbjct: 472 GDRVKINVDASFDPDFLRGTTGAVIRDKKGKFIAACNARLDMVQDVLSAEALALKHGLFL 531

Query: 65  TSIRNIRKVMVCSDSLSLISILRKD-------DRCHADCFPVVAYIQSFFSSFEKINFCH 124
                  +V+V SD+  LI I++ +          + DCF       S  S F ++ + H
Sbjct: 532 AKSMGCNRVVVSSDNSELIEIMKNERPPTGIAAAIYHDCF-------SMESDFVRVKYEH 591

Query: 125 INREYNLLSHELASLGMDSTTRVW 142
            NRE N ++HELA L    T+RVW
Sbjct: 592 ANRETNSVTHELAQLAKFQTSRVW 608

BLAST of Lag0006195 vs. NCBI nr
Match: XP_030969948.1 (uncharacterized protein LOC115990241 [Quercus lobata])

HSP 1 Score: 64.7 bits (156), Expect = 8.8e-07
Identity = 39/121 (32.23%), Postives = 67/121 (55.37%), Query Frame = 0

Query: 10  LHVDAAWTKHNKTGGAKVVLRTKMGKLVAIMQKGIPLPSSPLCVEEIVVLEGLKMTSIRN 69
           +++D A  + + T G +VV+R + G+++A + + I LPSS L VE +    GL++     
Sbjct: 64  VNMDGALFEADNTAGPRVVIRNEHGQVMASLSERIRLPSSVLEVEALAARRGLELVVETG 123

Query: 70  IRKVMVCSDSLSLISILRKDDRCHADCFPVVAYIQSFFSSFEKINFCHINREYNLLSHEL 129
            R +++ SDS  LI+ LR+     +    +V  I+   S    IN+ H+ R+ N L+H L
Sbjct: 124 FRNIVLESDSQILITTLREGSYSLSSFGHIVQDIKFIASYVSSINYTHVRRQCNALAHSL 183

Query: 130 A 131
           A
Sbjct: 184 A 184

BLAST of Lag0006195 vs. NCBI nr
Match: CAB4280156.1 (unnamed protein product [Prunus armeniaca])

HSP 1 Score: 64.3 bits (155), Expect = 1.2e-06
Identity = 42/126 (33.33%), Postives = 71/126 (56.35%), Query Frame = 0

Query: 8   IILHVDAAWTKHNKTGGAKVVLRTKMGKLVAIMQKGIPLPSSPLCVEEIVVLEGLKMTSI 67
           I ++VD AW K +  GG    LR   GKL+A M K   L +S + VE   VLEGL +   
Sbjct: 92  IKVNVDGAWKKDSLQGGVGCCLRDSWGKLLAGMSKA-TLRNSAVEVEAEAVLEGLILAKE 151

Query: 68  RNIRKVMVCSDSLSLISILRKDD-RCHADCFPVVAYIQSFFSSFEKINFCHINREYNLLS 127
           +N +K+++ SDS+ ++S +R ++ R +    P++  I+   S F  + +  ++R+ N  +
Sbjct: 152 KNCQKIILESDSMDVVSCMRNENLRGNWRIIPIILEIRRLSSWFTSVKWEWVSRQANQAA 211

Query: 128 HELASL 133
           H  A+L
Sbjct: 212 HAAAAL 216

BLAST of Lag0006195 vs. NCBI nr
Match: RYR10083.1 (hypothetical protein Ahy_B05g078550 [Arachis hypogaea])

HSP 1 Score: 63.5 bits (153), Expect = 2.0e-06
Identity = 42/133 (31.58%), Postives = 66/133 (49.62%), Query Frame = 0

Query: 10  LHVDAAWTKHNKTGGAKVVLRTKMGKLVAIMQKGIPLPSSPLCVEEIVVLEGLKMTSIRN 69
           ++VDAA   H+  GG  VV+R +MG ++A   + IP P      E      GL       
Sbjct: 180 VNVDAA-VPHDINGGVGVVIRNEMGIIMAAATQAIPYPLEEHEAEAYAAFWGLNFAKDCC 239

Query: 70  IRKVMVCSDSLSLISILRKDDRCHADCF-PVVAYIQSFFSSFEKINFCHINREYNLLSHE 129
             KV++ SD++ ++  L K  RC   CF   +    S   +F  + F H+ RE N ++HE
Sbjct: 240 FSKVILESDNIEVMGAL-KQRRCFNSCFGTFITDAISLIRNFRFVEFSHVKREGNKVAHE 299

Query: 130 LASLGMDSTTRVW 142
           LA+L + +   +W
Sbjct: 300 LATLALTTPNCMW 310

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_016199994.12.3e-0730.77uncharacterized protein LOC107641002 [Arachis ipaensis] >XP_025652971.1 uncharac... [more]
VAH31913.16.8e-0729.17unnamed protein product [Triticum turgidum subsp. durum][more]
XP_030969948.18.8e-0732.23uncharacterized protein LOC115990241 [Quercus lobata][more]
CAB4280156.11.2e-0633.33unnamed protein product [Prunus armeniaca][more]
RYR10083.12.0e-0631.58hypothetical protein Ahy_B05g078550 [Arachis hypogaea][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 12..132
e-value: 6.7E-17
score: 61.5
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 6..137
e-value: 1.2E-12
score: 50.0
NoneNo IPR availablePANTHERPTHR33033POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATEDcoord: 11..149
NoneNo IPR availablePANTHERPTHR33033:SF67SUBFAMILY NOT NAMEDcoord: 11..149
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 10..130
e-value: 3.95002E-14
score: 63.4872
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 7..137

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0006195.1Lag0006195.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity