Lag0006163 (gene) Sponge gourd (AG‐4) v1

BLAST of Lag0006163 vs. NCBI nr
Match: PRQ55763.1 (putative RNA-directed DNA polymerase [Rosa chinensis])

HSP 1 Score: 325.9 bits (834), Expect = 6.2e-85
Identity = 206/652 (31.60%), Postives = 292/652 (44.79%), Query Frame = 0

Query: 36  SLERQLDFLLLEEEIYWKQRSRESWLKWGDRNTKWFHQKATSRRQKN-----------WC 95
           +LE +L+ LLL+E+ +WKQR++  WL  GD NT++FHQ+A++RR+KN           WC
Sbjct: 171 ALEDRLNELLLKEQDFWKQRAKVFWLSEGDLNTRFFHQRASNRRKKNRIKGLLDENGTWC 230

Query: 96  ------------------------DLDAVLGFIPRKVTETMNQMLTTPYSREEVVAVIKS 155
                                    +D V   +P  +TE MN +L    + +EV   +K 
Sbjct: 231 TADGEIENIVLNYYRTLFTSSQPAGMDDVASVLPCFITEEMNNILAGDITFDEVYKALKQ 290

Query: 156 FHPTKAPGPDGFPALFIKDILRL-------------------WEWNHTNIVLIPKIR--- 215
            HP KAPGPDGF   F +    L                    + N TN+ LIPK++   
Sbjct: 291 MHPAKAPGPDGFAPCFYQHFWYLVGNDVTMAVRCFMESDGMISQINCTNVTLIPKVKVPE 350

Query: 216 ---QLKS---------------------------------------ITDNMILGHETLHF 275
              QL+                                        I+DN +   E  HF
Sbjct: 351 HVNQLRPISLCNVIYKIGSKVLANRLKPLLQDLISPYQSAFVPGRLISDNSLAAFEISHF 410

Query: 276 LNQKCKGRVGYAALKLDMSKAYDEWSEGF----------------------------FVM 335
           L ++ +G VGY ALKLDMSKAYD    GF                            F++
Sbjct: 411 LKRRRRGHVGYGALKLDMSKAYDRVEWGFLDRVMQQLGFCPKWRGWVMRCVTTVSYSFIV 470

Query: 336 LVSARNR------------------------------------SMAGVSLAQTCPKISHL 395
             + R R                                    S+ GV +    P ISHL
Sbjct: 471 NGTPRGRVVPSRGLRQGDSISPYLFLICAETLSRLILKAEDMGSIHGVRICTGAPPISHL 530

Query: 396 FFADDSLVFFKVAAEDFGFFKTILKAYEKASGQCVNLSKSMVCFSRNVFDASEQYLSQIL 455
           FFADDS +FFK   ++    K + + YEKASGQ +N  KS V FS+NV  A +  L+  L
Sbjct: 531 FFADDSFIFFKAETDECDVLKRMFEQYEKASGQMINFDKSCVSFSKNVKRAKQDELAASL 590

Query: 456 SMKVSESLGNYLGLPSSFHKGKSRDFQFLLDK---------------------------A 498
            +   +    YLGLP+     KS  F FL +K                           +
Sbjct: 591 RVLRVDKHDKYLGLPTEISYSKSEAFNFLSEKVRKRTQGWRAKTLSAAGKEIMIKAVAQS 650

BLAST of Lag0006163 vs. NCBI nr
Match: XP_022158377.1 (uncharacterized protein LOC111024874 [Momordica charantia])

HSP 1 Score: 324.3 bits (830), Expect = 1.8e-84
Identity = 202/595 (33.95%), Postives = 284/595 (47.73%), Query Frame = 0

Query: 27   LSIDFELVHSLERQLDFLLLEEEIYWKQRSRESWLKWGDRNTKWFHQKATSRRQKNWCDL 86
            L +DF ++H+LE  L  LL  EEI+WKQRSRE WLKWG                 N  D+
Sbjct: 521  LPLDFTIIHALENDLAGLLELEEIFWKQRSREDWLKWGIAIL-------------NALDI 580

Query: 87   DAVLGFIPRKVTETMNQMLTTPYSREEVVAVIKSFHPTKAPGPDGFPALFIKDILR---- 146
            +A++  IP ++T  +N+ L  PY++EE+   I+   PTKA GPDGFPALF +        
Sbjct: 581  EAIINLIPTRITSEVNEQLLAPYTKEEIELAIRQMFPTKALGPDGFPALFYQTYWHVVGP 640

Query: 147  ---------------LWEWNHTNIVLIPKIRQLKS------------------------- 206
                           + +WN T I LIPKI+Q +S                         
Sbjct: 641  KTLEACLNALNNGDDIKKWNSTYIALIPKIKQPRSISDFRPISLCNVSYKIISKSITNRL 700

Query: 207  --------------------ITDNMILGHETLHFLNQKCKGRVGYAALKLDMSKAYD--E 266
                                I+DN+I+GHE LH +N    G +G AALKLD+SKA+D  E
Sbjct: 701  KNVIGLVISDAQSAFVPSRAISDNVIIGHECLHTINSCKSGLIGMAALKLDLSKAFDRVE 760

Query: 267  W------------SEGF-----------------------------------------FV 326
            W            +EG+                                         F+
Sbjct: 761  WTYLECIMRKMGFNEGWIQAIIQCISTVRFSIHLNGSPGGCFQPSRGIRQGDPLSPYLFL 820

Query: 327  M-------LVSARNRS--MAGVSLAQTCPKISHLFFADDSLVFFKVAAEDFGFFKTILKA 386
            +       L++  N S  + G+   +    I+HL FADDSL+F +    +    + +L +
Sbjct: 821  LCAEGLSALINHENNSGRLTGIHFEENNTSITHLLFADDSLIFLRSLESECLALRRLLDS 880

Query: 387  YEKASGQCVNLSKSMVCFSRNVFDASEQYLSQILSMKVSESLGNYLGLPSSFHKGKSRDF 446
            Y +ASGQC+N SKS + FS NV    +QYL  IL++K+    GNYLGLPS F + +    
Sbjct: 881  YGRASGQCINFSKSALLFSPNVHPERQQYLQCILNVKLVSHFGNYLGLPSHFTRRR---- 940

Query: 447  QFLLDKAIPTYVMGCFKIPKGILNKIAALCAKFWWGSDGDHKRVHWKRWEDLCKPKESGG 494
                                                  G+ +++HW +W  +C PKE GG
Sbjct: 941  --------------------------------------GESRKLHWMKWGRMCYPKECGG 1000

BLAST of Lag0006163 vs. NCBI nr
Match: XP_023878301.1 (uncharacterized protein LOC111990748 [Quercus suber])

HSP 1 Score: 317.4 bits (812), Expect = 2.2e-82
Identity = 203/667 (30.43%), Postives = 296/667 (44.38%), Query Frame = 0

Query: 6   TTVYNR--KAVRHHSSLSSSESGLSIDFEL---VHSLERQLDFLLLEEEIYWKQRSRESW 65
           +T+Y +  K ++   S  ++ +   +D +L   ++ L  +++ LL +EE YW QR++  W
Sbjct: 249 STIYGQIPKKIQDKRSRLNTLAMRELDEDLSLEINRLREEINALLDDEETYWGQRAKAHW 308

Query: 66  LKWGDRNTKWFHQKATSRRQKN-----------WCD------------------------ 125
           LK GDRNTK+FH +A+ RR++N           WCD                        
Sbjct: 309 LKEGDRNTKFFHAQASERRKQNTIVGIWDEQGRWCDNEESIAQAAISYFNNIYSSSHPSQ 368

Query: 126 LDAVLGFIPRKVTETMNQMLTTPYSREEVVAVIKSFHPTKAPGPDGFPALFIKDI----- 185
           ++ V   IP KVTE MN+ L   +++EEV   +K  HP KAPGPDG  A+F +       
Sbjct: 369 IEEVTEAIPFKVTEEMNESLIREFTKEEVAVALKQIHPNKAPGPDGMSAVFFQKYWSIVG 428

Query: 186 --------------LRLWEWNHTNIVLIPKIRQLKS------------------------ 245
                         L + E N TNI LIPK    K                         
Sbjct: 429 NNVTDMVLNVLNHNLPIPELNKTNISLIPKTNNPKRMTDFRPISLCNVVYKLISKILANR 488

Query: 246 ---------------------ITDNMILGHETLHFLNQKCKGRVGYAALKLDMSKAYDEW 305
                                ITDN+++  E +H+L+ K  G+ G+ A+KLDMSKA+D  
Sbjct: 489 LKPLLPHIISENQSAFTSDRLITDNVLVAFELMHYLDHKTAGKEGFMAIKLDMSKAFDRV 548

Query: 306 SEGFFVMLV--------------------------------------------------- 365
             GF   ++                                                   
Sbjct: 549 EWGFIAKVMEQMGFCNRWRDLVMQCITSVSYSILINGVAHGNIYPSRGLRQGDPLSPSLF 608

Query: 366 -------------SARNRSMAGVSLAQTCPKISHLFFADDSLVFFKVAAEDFGFFKTILK 425
                        +ARN+ + G+S+ + CPK++HLFFADDS++F K A E+    ++IL 
Sbjct: 609 LLCAEGLSALINQAARNKLITGISINRGCPKVTHLFFADDSILFCKAAYEECHLLRSILG 668

Query: 426 AYEKASGQCVNLSKSMVCFSRNVFDASEQYLSQILSMKVSESLGNYLGLPSSFHKGKSRD 478
            YE+ASGQ +N  KS + FS N    +   +  IL    +     YLGLPS   + KS+ 
Sbjct: 669 QYEEASGQKINTDKSSIFFSPNTAQETRDEIFNILGPMQNSRHTKYLGLPSLIGRSKSQV 728

BLAST of Lag0006163 vs. NCBI nr
Match: XP_023881891.1 (uncharacterized protein LOC111994244 [Quercus suber])

HSP 1 Score: 317.0 bits (811), Expect = 2.9e-82
Identity = 204/635 (32.13%), Postives = 289/635 (45.51%), Query Frame = 0

Query: 34  VHSLERQLDFLLLEEEIYWKQRSRESWLKWGDRNTKWFHQKATSRRQK-----------N 93
           ++ L ++++ LL  EEI W+QRSR  WL  GDRNTK+FH KA+ RR++           N
Sbjct: 158 INMLRKEINELLDSEEIKWQQRSRVQWLGLGDRNTKYFHTKASDRRRRNTINGIMDENGN 217

Query: 94  WCD------------------------LDAVLGFIPRKVTETMNQMLTTPYSREEVVAVI 153
           W D                        +  VL  IP  VTE MN  L   ++REE+   +
Sbjct: 218 WQDSTEGIAKVAVSYFQTIYSSSVPTRISEVLDAIPTTVTEEMNHSLIQEFTREEIETAL 277

Query: 154 KSFHPTKAPGPDGFPALF---------------IKDILR----LWEWNHTNIVLIPKIR- 213
              HPTKAPGPDG  A+F               + D+L     + E N TNI L+PKI+ 
Sbjct: 278 NQMHPTKAPGPDGMSAIFFQKYWNIVGNDIVCMVLDVLNSNMSMVEINKTNITLVPKIKN 337

Query: 214 --------------------------QLKS------------------ITDNMILGHETL 273
                                     +LK+                  ITDN+++  E +
Sbjct: 338 PTKMSDFRPISLCNVVYKLISKVLANRLKNILPQIISENQSAFLSGRLITDNVLVAFELM 397

Query: 274 HFLNQKCKGRVGYAALKLDMSKAYD--EW------------------------------- 333
           H+L  K +G+ G+AA+KLDMSKAYD  EW                               
Sbjct: 398 HYLEHKKEGKEGFAAIKLDMSKAYDRVEWGFIKQVMEKMGFHEKWIKLVMHCITSVSYSI 457

Query: 334 ------------------------------SEGFFVMLVS-ARNRSMAGVSLAQTCPKIS 393
                                         ++GF  +L   AR   ++GVS+ + CPKI+
Sbjct: 458 LVNGGAYGSITPTRGLRQGDPISPYIFLLCADGFSSLLNDVARKLRISGVSICRGCPKIT 517

Query: 394 HLFFADDSLVFFKVAAEDFGFFKTILKAYEKASGQCVNLSKSMVCFSRNVFDASEQYLSQ 453
           HLFFADDSL+F K  +++      IL+ YE ASGQ +N+ KS V FS N  D     + +
Sbjct: 518 HLFFADDSLLFCKANSQECQTLIDILQLYEDASGQKINVDKSSVFFSNNTPDEKRCEVLR 577

Query: 454 ILSMKVSESLGNYLGLPSSFHKGKSRDFQFLLDK-------------------------- 479
           +L          YLGLPS   K K   F  + ++                          
Sbjct: 578 MLGHMQDTRHKKYLGLPSIIGKSKVEIFAEVKERVERKLSGWKEKLLSVGGREILIKAVA 637

BLAST of Lag0006163 vs. NCBI nr
Match: XP_024172256.1 (uncharacterized protein LOC112178331 [Rosa chinensis])

HSP 1 Score: 316.6 bits (810), Expect = 3.8e-82
Identity = 198/609 (32.51%), Postives = 286/609 (46.96%), Query Frame = 0

Query: 37  LERQLDFLLLEEEIYWKQRSRESWLKWGDRNTKWFHQKATSRRQKN-----------WCD 96
           L  +L+ LL EE  YW+QRS+ SWL  GDRNTK+FH KA++RR KN           W D
Sbjct: 12  LHSRLEGLLAEESAYWRQRSKVSWLTGGDRNTKYFHWKASNRRAKNRLFGLFDHNGVWQD 71

Query: 97  --------------------------LDAVLGFIPRKVTETMNQMLTTPYSREEVVAVIK 156
                                     + +V+  +  KVT  MN  L  PYS  E+   + 
Sbjct: 72  SMKGMEEVVLRYFTGLFKANDINYIHMQSVVELVQPKVTTEMNANLCAPYSAAEIKTALF 131

Query: 157 SFHPTKAPGPDGFPALFIK--------DILR-----------LWEWNHTNIVLIPKIRQL 216
             +PTKAPGPDG P LF +        D++            L E N+T+I LIPK++ L
Sbjct: 132 QMYPTKAPGPDGMPPLFFQKYWEIVGSDVVSAVQHFLHTGQFLGEINYTHICLIPKVKDL 191

Query: 217 KS--------ITDNMILGHETLHFLNQKCKGRVGYAALKLDMSKAYD--EWS-------- 276
            S        ITDN ++ +E  HF++       G  +LKLDMSKAYD  EWS        
Sbjct: 192 VSSAFIPGRLITDNTLVANEVSHFIHNCYSSSEGVFSLKLDMSKAYDRMEWSFLEAMLYR 251

Query: 277 EGF----------------FVMLVSARNR------------------------------- 336
            GF                +  L++ + R                               
Sbjct: 252 LGFDENWIGVIMKCVTTVRYSFLINGQPRGYLTPTRGLRQGDPLSPYLFLLCAEVFSALL 311

Query: 337 -------SMAGVSLAQTCPKISHLFFADDSLVFFKVAAEDFGFFKTILKAYEKASGQCVN 396
                   + G+ + +  P I HL FADDSLVF K    +    +++L AYE ASGQ +N
Sbjct: 312 ARKVTHGELQGIKICEGAPVIHHLLFADDSLVFGKATLSECQHIQSVLNAYELASGQQIN 371

Query: 397 LSKSMVCFSRNVFDASEQYLSQILSMKVSESLGNYLGLPSSFHKGKSRDFQFLLDK---- 456
            +KS + FS+ V +A +  L+  L + +      YLGLP+   + ++  F ++ ++    
Sbjct: 372 FAKSSIVFSKRVPEADKLSLASFLGVAIEVKHEKYLGLPTYLGRNRTETFAYIKERLSKK 431

Query: 457 -----------------------AIPTYVMGCFKIPKGILNKIAALCAKFWWGSDGDHKR 488
                                  A+P+Y M CF +P    + +  +C+KFWWGS  + ++
Sbjct: 432 LAGWQGKLLSGAGKDLLVRVVAQALPSYAMSCFLLPNNFCDDLHQMCSKFWWGSKPNERK 491

BLAST of Lag0006163 vs. ExPASy Swiss-Prot
Match: P93295 (Uncharacterized mitochondrial protein AtMg00310 OS=Arabidopsis thaliana OX=3702 GN=AtMg00310 PE=4 SV=1)

HSP 1 Score: 125.2 bits (313), Expect = 2.1e-27
Identity = 53/143 (37.06%), Postives = 89/143 (62.24%), Query Frame = 0

Query: 325 AIPTYVMGCFKIPKGILNKIAALCAKFWWGSDGDHKRVHWKRWEDLCKPKE-SGGLNFRE 384
           A+P Y M CF++ K +  K+ +   +FWW S  + +++ W  W+ LCK KE  GGL FR+
Sbjct: 2   ALPVYAMSCFRLSKLLCKKLTSAMTEFWWSSCENKRKISWVAWQKLCKSKEDDGGLGFRD 61

Query: 385 LVNFNQAMLAKQAWRVLSNPNLTVSRVLSGRYFQDSSVLNVTSRPNSSFFRKGFVWGMEL 444
           L  FNQA+LAKQ++R++  P+  +SR+L  RYF  SS++  +     S+  +  + G EL
Sbjct: 62  LGWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHGREL 121

Query: 445 LKKGIRKNLGNGHSIRMFRDPWL 467
           L +G+ + +G+G   +++ D W+
Sbjct: 122 LSRGLLRTIGDGIHTKVWLDRWI 144

BLAST of Lag0006163 vs. ExPASy Swiss-Prot
Match: P0C2F6 (Putative ribonuclease H protein At1g65750 OS=Arabidopsis thaliana OX=3702 GN=At1g65750 PE=3 SV=1)

HSP 1 Score: 84.7 bits (208), Expect = 3.2e-15
Identity = 44/146 (30.14%), Postives = 75/146 (51.37%), Query Frame = 0

Query: 325 AIPTYVMGCFKIPKGILNKIAALCAKFWWGSDGDHKRVHWKRWEDLCKPKESGGLNFREL 384
           ++P + M    +P+ ILN++  L   F WGS  + K+ H  +W  +C PK+ GGL  R  
Sbjct: 47  SMPVHSMSTILLPQSILNRLDQLSRTFLWGSTAEKKKQHLVKWSKVCSPKKEGGLGVRAA 106

Query: 385 VNFNQAMLAKQAWRVLSNPNLTVSRVLSGRY----FQDSSVLNVTSRPNSSFFRKGFVWG 444
            + N+A+++K  WR+L   N   + VL  +Y     +DS  L +     SS +R   +  
Sbjct: 107 KSMNRALISKVGWRLLQEKNSLWTLVLQKKYHVGEIRDSRWL-IPKGSWSSTWRSIAIGL 166

Query: 445 MELLKKGIRKNLGNGHSIRMFRDPWL 467
            +++  G+    G+G  IR + D W+
Sbjct: 167 RDVVSHGVGWIPGDGQQIRFWTDRWV 191

BLAST of Lag0006163 vs. ExPASy TrEMBL
Match: A0A803PWX1 (Uncharacterized protein OS=Cannabis sativa OX=3483 PE=4 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 2.1e-86
Identity = 209/654 (31.96%), Postives = 296/654 (45.26%), Query Frame = 0

Query: 31   FELVHSLERQLDFLLLEEEIYWKQRSRESWLKWGDRNTKWFHQKATSRRQKN-------- 90
            +E +  +E +L+ LL ++E YW+QRSR  WL+WGDRNTK+FH KA+SRR+KN        
Sbjct: 861  WEAIQHMEDKLNGLLEKDEQYWRQRSRALWLQWGDRNTKYFHHKASSRRKKNEIKGLQDQ 920

Query: 91   ---WCD--------------------------LDAVLGFIPRKVTETMNQMLTTPYSREE 150
               W D                          +  VL  +  KV+ +MN+ L   +S EE
Sbjct: 921  MGVWQDDKLLVCQIVEDYYKGLFMGSDIDQGVMQEVLEVVQPKVSMSMNEELMVDFSVEE 980

Query: 151  VVAVIKSFHPTKAPGPDGFPALFIKDILRLWE----------------------WNHTNI 210
            VV  +K  +PTKAPG DG PALF +   + W                        N T +
Sbjct: 981  VVQAVKGMNPTKAPGADGLPALFYQ---KFWSKLKDEVIAVCLNVLNNGADLSCLNDTVV 1040

Query: 211  VLIPKIRQLKSIT---------------------------------------------DN 270
             LIPK+ + + I                                              DN
Sbjct: 1041 ALIPKVDKPQKIEEFRPISLCNVIYKIVSKCLANRLRHSLDQVVSDSQSAFLKGRLIHDN 1100

Query: 271  MILGHETLHFLNQKCKGRVGYAALKLDMSKAYD--EW----------------------- 330
             I+G+E LH + +         ALKLDM+KAYD  EW                       
Sbjct: 1101 AIVGYECLHVMRKNRFRNGTKVALKLDMAKAYDRVEWRFLEAMMLKLGYDVPWVSKIMRC 1160

Query: 331  --------------------------------------SEGFFVMLVSARNR-SMAGVSL 390
                                                  +E F  ++  A +   + G+  
Sbjct: 1161 LTSVQFSFLINGEIQGKVTPQRGLRQGDPLSPFLFLLCAEAFSCLIQHAEHSGQLHGIGF 1220

Query: 391  AQTCPKISHLFFADDSLVFFKVAAEDFGFFKTILKAYEKASGQCVNLSKSMVCFSRNVFD 450
             +    +SHLFFADDSLVF     ++   FK +L+ Y KASGQ VN  KS +CF R V  
Sbjct: 1221 GRQGVSVSHLFFADDSLVFLAANEDECKGFKELLEKYSKASGQLVNFHKSEMCFGRKVTL 1280

Query: 451  ASEQYLSQILSMKVSESLGNYLGLPSSFHKGKSRDFQFLLDK------------------ 490
                +L+ I+ +KV ++ G YLGLPS   + K + F+F+ +K                  
Sbjct: 1281 PVRTHLANIMGVKVVDNYGKYLGLPSFVGRTKKQHFEFIKNKVWNKLKGWKGSFFSAAGK 1340

BLAST of Lag0006163 vs. ExPASy TrEMBL
Match: A0A2P6SAP1 (Putative RNA-directed DNA polymerase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr1g0328181 PE=4 SV=1)

HSP 1 Score: 325.9 bits (834), Expect = 3.0e-85
Identity = 206/652 (31.60%), Postives = 292/652 (44.79%), Query Frame = 0

Query: 36  SLERQLDFLLLEEEIYWKQRSRESWLKWGDRNTKWFHQKATSRRQKN-----------WC 95
           +LE +L+ LLL+E+ +WKQR++  WL  GD NT++FHQ+A++RR+KN           WC
Sbjct: 171 ALEDRLNELLLKEQDFWKQRAKVFWLSEGDLNTRFFHQRASNRRKKNRIKGLLDENGTWC 230

Query: 96  ------------------------DLDAVLGFIPRKVTETMNQMLTTPYSREEVVAVIKS 155
                                    +D V   +P  +TE MN +L    + +EV   +K 
Sbjct: 231 TADGEIENIVLNYYRTLFTSSQPAGMDDVASVLPCFITEEMNNILAGDITFDEVYKALKQ 290

Query: 156 FHPTKAPGPDGFPALFIKDILRL-------------------WEWNHTNIVLIPKIR--- 215
            HP KAPGPDGF   F +    L                    + N TN+ LIPK++   
Sbjct: 291 MHPAKAPGPDGFAPCFYQHFWYLVGNDVTMAVRCFMESDGMISQINCTNVTLIPKVKVPE 350

Query: 216 ---QLKS---------------------------------------ITDNMILGHETLHF 275
              QL+                                        I+DN +   E  HF
Sbjct: 351 HVNQLRPISLCNVIYKIGSKVLANRLKPLLQDLISPYQSAFVPGRLISDNSLAAFEISHF 410

Query: 276 LNQKCKGRVGYAALKLDMSKAYDEWSEGF----------------------------FVM 335
           L ++ +G VGY ALKLDMSKAYD    GF                            F++
Sbjct: 411 LKRRRRGHVGYGALKLDMSKAYDRVEWGFLDRVMQQLGFCPKWRGWVMRCVTTVSYSFIV 470

Query: 336 LVSARNR------------------------------------SMAGVSLAQTCPKISHL 395
             + R R                                    S+ GV +    P ISHL
Sbjct: 471 NGTPRGRVVPSRGLRQGDSISPYLFLICAETLSRLILKAEDMGSIHGVRICTGAPPISHL 530

Query: 396 FFADDSLVFFKVAAEDFGFFKTILKAYEKASGQCVNLSKSMVCFSRNVFDASEQYLSQIL 455
           FFADDS +FFK   ++    K + + YEKASGQ +N  KS V FS+NV  A +  L+  L
Sbjct: 531 FFADDSFIFFKAETDECDVLKRMFEQYEKASGQMINFDKSCVSFSKNVKRAKQDELAASL 590

Query: 456 SMKVSESLGNYLGLPSSFHKGKSRDFQFLLDK---------------------------A 498
            +   +    YLGLP+     KS  F FL +K                           +
Sbjct: 591 RVLRVDKHDKYLGLPTEISYSKSEAFNFLSEKVRKRTQGWRAKTLSAAGKEIMIKAVAQS 650

BLAST of Lag0006163 vs. ExPASy TrEMBL
Match: A0A6J1DX30 (uncharacterized protein LOC111024874 OS=Momordica charantia OX=3673 GN=LOC111024874 PE=4 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 8.8e-85
Identity = 202/595 (33.95%), Postives = 284/595 (47.73%), Query Frame = 0

Query: 27   LSIDFELVHSLERQLDFLLLEEEIYWKQRSRESWLKWGDRNTKWFHQKATSRRQKNWCDL 86
            L +DF ++H+LE  L  LL  EEI+WKQRSRE WLKWG                 N  D+
Sbjct: 521  LPLDFTIIHALENDLAGLLELEEIFWKQRSREDWLKWGIAIL-------------NALDI 580

Query: 87   DAVLGFIPRKVTETMNQMLTTPYSREEVVAVIKSFHPTKAPGPDGFPALFIKDILR---- 146
            +A++  IP ++T  +N+ L  PY++EE+   I+   PTKA GPDGFPALF +        
Sbjct: 581  EAIINLIPTRITSEVNEQLLAPYTKEEIELAIRQMFPTKALGPDGFPALFYQTYWHVVGP 640

Query: 147  ---------------LWEWNHTNIVLIPKIRQLKS------------------------- 206
                           + +WN T I LIPKI+Q +S                         
Sbjct: 641  KTLEACLNALNNGDDIKKWNSTYIALIPKIKQPRSISDFRPISLCNVSYKIISKSITNRL 700

Query: 207  --------------------ITDNMILGHETLHFLNQKCKGRVGYAALKLDMSKAYD--E 266
                                I+DN+I+GHE LH +N    G +G AALKLD+SKA+D  E
Sbjct: 701  KNVIGLVISDAQSAFVPSRAISDNVIIGHECLHTINSCKSGLIGMAALKLDLSKAFDRVE 760

Query: 267  W------------SEGF-----------------------------------------FV 326
            W            +EG+                                         F+
Sbjct: 761  WTYLECIMRKMGFNEGWIQAIIQCISTVRFSIHLNGSPGGCFQPSRGIRQGDPLSPYLFL 820

Query: 327  M-------LVSARNRS--MAGVSLAQTCPKISHLFFADDSLVFFKVAAEDFGFFKTILKA 386
            +       L++  N S  + G+   +    I+HL FADDSL+F +    +    + +L +
Sbjct: 821  LCAEGLSALINHENNSGRLTGIHFEENNTSITHLLFADDSLIFLRSLESECLALRRLLDS 880

Query: 387  YEKASGQCVNLSKSMVCFSRNVFDASEQYLSQILSMKVSESLGNYLGLPSSFHKGKSRDF 446
            Y +ASGQC+N SKS + FS NV    +QYL  IL++K+    GNYLGLPS F + +    
Sbjct: 881  YGRASGQCINFSKSALLFSPNVHPERQQYLQCILNVKLVSHFGNYLGLPSHFTRRR---- 940

Query: 447  QFLLDKAIPTYVMGCFKIPKGILNKIAALCAKFWWGSDGDHKRVHWKRWEDLCKPKESGG 494
                                                  G+ +++HW +W  +C PKE GG
Sbjct: 941  --------------------------------------GESRKLHWMKWGRMCYPKECGG 1000

BLAST of Lag0006163 vs. ExPASy TrEMBL
Match: A0A7N2L6Z9 (Reverse transcriptase domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 319.7 bits (818), Expect = 2.2e-83
Identity = 206/641 (32.14%), Postives = 292/641 (45.55%), Query Frame = 0

Query: 34  VHSLERQLDFLLLEEEIYWKQRSRESWLKWGDRNTKWFHQKATSRRQKN----------- 93
           ++ + R+L+ LL +EEI+W QRS+  WLK GDRNTK+FH +A+ RR++N           
Sbjct: 197 INIVRRELNGLLDDEEIFWGQRSKVHWLKEGDRNTKFFHARASERRKQNTISGVWDKFGR 256

Query: 94  WCD------------------------LDAVLGFIPRKVTETMNQMLTTPYSREEVVAVI 153
           WC+                        +D V   IP  +TE MN  L+  ++REE+V  +
Sbjct: 257 WCEDRDSIANAAVTYFEDIYSTSNPSMVDEVTAAIPTMITEEMNTELSRCFTREEIVTAL 316

Query: 154 KSFHPTKAPGPDGFPALFIKD------------ILRLWEW-------NHTNIVLIPK--- 213
           K  HPTK+PGPDG  A+F +             IL +  +       N TNIVLIPK   
Sbjct: 317 KQIHPTKSPGPDGMSAIFFQKYWDIVGSNVSNMILNVLNYGMSLDVINKTNIVLIPKTSN 376

Query: 214 ------------------------IRQLKS------------------ITDNMILGHETL 273
                                     +LK+                  ITDN+++ +E +
Sbjct: 377 PKRMTDFRPISLCNVIYKLISKTLANRLKAFLPLIITENQSAFTADRLITDNVLIAYELM 436

Query: 274 HFLNQKCKGRVGYAALKLDMSKAYD--EW------------SEGFFVMLV---------- 333
           H+L  K  G+  + A KLDMSKA+D  EW            +EG+  +++          
Sbjct: 437 HYLKHKKDGKDCFMATKLDMSKAFDRVEWGFIERVMRKMGFNEGWISLIMRCISSVSYSV 496

Query: 334 ----------------------------------------SARNRSMAGVSLAQTCPKIS 393
                                                   +ARN+ + G+SL + CP+I+
Sbjct: 497 IINGEYFGNIVPTRGLRQGDPLSPYLFLLCAEGLSALLHDAARNQLLNGISLCRGCPRIT 556

Query: 394 HLFFADDSLVFFKVAAEDFGFFKTILKAYEKASGQCVNLSKSMVCFSRNVFDASEQYLSQ 453
           HLFFADDSL+F K   E+    K IL+ YE ASGQ VN  KS + FS N     ++ +  
Sbjct: 557 HLFFADDSLLFCKANREECEKLKEILEKYEAASGQKVNSDKSSIFFSPNTTPELKETIFN 616

Query: 454 ILSMKVSESLGNYLGLPSSFHKGKSRDFQFLLDK-------------------------- 485
           IL          YLGLPS   + K   F  + ++                          
Sbjct: 617 ILGPMQDSRHNKYLGLPSIIGRSKKLVFAEIKERVGLKLAGWKGKLLSSGGKEILIKAVA 676

BLAST of Lag0006163 vs. ExPASy TrEMBL
Match: M5VU98 (Reverse transcriptase domain-containing protein OS=Prunus persica OX=3760 GN=PRUPE_ppa022115mg PE=4 SV=1)

HSP 1 Score: 318.2 bits (814), Expect = 6.3e-83
Identity = 200/650 (30.77%), Postives = 293/650 (45.08%), Query Frame = 0

Query: 37   LERQLDFLLLEEEIYWKQRSRESWLKWGDRNTKWFHQKATSRRQKN-----------W-- 96
            +++ LD LL + E+YW QRSRE+WLK GD+NT +FHQKAT+RR++N           W  
Sbjct: 739  VQKSLDELLAKNELYWCQRSRENWLKAGDKNTSYFHQKATNRRRRNIIKGLEDSNGCWRT 798

Query: 97   ----------------------CDLDAVLGFIPRKVTETMNQMLTTPYSREEVVAVIKSF 156
                                    ++ +L  +  KVT  M Q+L   +S +E+   +   
Sbjct: 799  SRQGITSIVIDYFGDLFRSSGSSMMEEILSALEPKVTADMQQVLIADFSYQEIKDAVFQM 858

Query: 157  HPTKAPGPDGFPALFIKDILR-------------------LWEWNHTNIVLIPKIRQLKS 216
             P+KAPGPDG P LF +   R                   L + NHT + LIPK+++ ++
Sbjct: 859  QPSKAPGPDGLPPLFYQKYWRIVGDDVVAAVRAFLQSNEMLRQLNHTFVTLIPKVKEPRT 918

Query: 217  ---------------------------------------------ITDNMILGHETLHFL 276
                                                         ITDN I+  E  HFL
Sbjct: 919  MAQLRPISLCNVLYRIGAKTLANRMKFVMQSVISESQSAFVPGRLITDNSIVAFEIAHFL 978

Query: 277  NQKCKGRVGYAALKLDMSKAYD--EW---------------------------------- 336
             Q+ +GR G  ALKLDMSKAYD  EW                                  
Sbjct: 979  KQRRRGRKGSLALKLDMSKAYDRVEWEFLEKMMLAMGFPILWVRMVMDCVTTVSYSFLVN 1038

Query: 337  ---------------------------SEGFFVMLVSA-RNRSMAGVSLAQTCPKISHLF 396
                                       +EGF  +L  A R   + G+ + +  P +SHLF
Sbjct: 1039 GEPTRILYPTRGLRQGDPLSPYLFLLCAEGFTTLLSKAERQGQLQGIVICRGAPTVSHLF 1098

Query: 397  FADDSLVFFKVAAEDFGFFKTILKAYEKASGQCVNLSKSMVCFSRNVFDASEQYLSQILS 456
            FADDS VF K    + G  K I + YE ASGQ +N  KS V FS N+   ++  L+ +L 
Sbjct: 1099 FADDSFVFAKATDNNCGVLKHIFEVYEHASGQQINCQKSCVAFSANIHMDTQSRLASVLG 1158

Query: 457  MKVSESLGNYLGLPSSFHKGKSRDFQFLLDK---------------------------AI 497
            +   +S   YLGLP    + K+  F++L ++                           +I
Sbjct: 1159 VPRVDSHATYLGLPMMLGRNKTVCFRYLKERVWKKLQGWREQTLSIAGKEVLLKVVAQSI 1218

BLAST of Lag0006163 vs. TAIR 10
Match: AT4G29090.1 (Ribonuclease H-like superfamily protein )

HSP 1 Score: 126.3 bits (316), Expect = 6.8e-29
Identity = 56/142 (39.44%), Postives = 81/142 (57.04%), Query Frame = 0

Query: 325 AIPTYVMGCFKIPKGILNKIAALCAKFWWGSDGDHKRVHWKRWEDLCKPKESGGLNFREL 384
           A+PTY M CF +PK +  +I ++ A FWW +  + K +HWK W+ L   K  GG+ F+++
Sbjct: 2   ALPTYTMACFLLPKTVCKQIISVLADFWWRNKQEAKGMHWKAWDHLSCYKAEGGIGFKDI 61

Query: 385 VNFNQAMLAKQAWRVLSNPNLTVSRVLSGRYFQDSSVLNVTSRPNSSFFRKGFVWGMELL 444
             FN A+L KQ WR+LS P   +++V   RYF  S  LN       SF  K      E+L
Sbjct: 62  EAFNLALLGKQMWRMLSRPESLMAKVFKSRYFHKSDPLNAPLGSRPSFVWKSIHASQEIL 121

Query: 445 KKGIRKNLGNGHSIRMFRDPWL 467
           ++G R  +GNG  I ++R  WL
Sbjct: 122 RQGARAVVGNGEDIIIWRHKWL 143

BLAST of Lag0006163 vs. TAIR 10
Match: ATMG00310.1 (RNA-directed DNA polymerase (reverse transcriptase)-related family protein )

HSP 1 Score: 125.2 bits (313), Expect = 1.5e-28
Identity = 53/143 (37.06%), Postives = 89/143 (62.24%), Query Frame = 0

Query: 325 AIPTYVMGCFKIPKGILNKIAALCAKFWWGSDGDHKRVHWKRWEDLCKPKE-SGGLNFRE 384
           A+P Y M CF++ K +  K+ +   +FWW S  + +++ W  W+ LCK KE  GGL FR+
Sbjct: 2   ALPVYAMSCFRLSKLLCKKLTSAMTEFWWSSCENKRKISWVAWQKLCKSKEDDGGLGFRD 61

Query: 385 LVNFNQAMLAKQAWRVLSNPNLTVSRVLSGRYFQDSSVLNVTSRPNSSFFRKGFVWGMEL 444
           L  FNQA+LAKQ++R++  P+  +SR+L  RYF  SS++  +     S+  +  + G EL
Sbjct: 62  LGWFNQALLAKQSFRIIHQPHTLLSRLLRSRYFPHSSMMECSVGTRPSYAWRSIIHGREL 121

Query: 445 LKKGIRKNLGNGHSIRMFRDPWL 467
           L +G+ + +G+G   +++ D W+
Sbjct: 122 LSRGLLRTIGDGIHTKVWLDRWI 144

BLAST of Lag0006163 vs. TAIR 10
Match: AT1G43760.1 (DNAse I-like superfamily protein )

HSP 1 Score: 48.9 bits (115), Expect = 1.4e-05
Identity = 42/185 (22.70%), Postives = 70/185 (37.84%), Query Frame = 0

Query: 31  FELVHSLERQLDFLLLEEEIYWKQRSRESWLKWGDRNTKWFHQKATSRRQKNWC------ 90
           F + H   ++ +F     E +++Q+SR  WL+ GD NT++FH+   + + KN        
Sbjct: 414 FRVEHVARKKWNFFAAALESFYRQKSRIKWLQDGDANTRFFHKVILANQAKNLIKFLRMD 473

Query: 91  ---------------------------------DLDAVLGFIPRKVTETMNQMLTTPYSR 150
                                             +  +    P +  +T+   L+   S 
Sbjct: 474 DDVRVENVTQVKEMIVAYYTHLLGSDSDILTPDSVQRIKDIHPFRCNDTLASRLSALPSD 533

Query: 151 EEVVAVIKSFHPTKAPGPDGFPALF---------------IKDILR----LWEWNHTNIV 158
           +E+ A + +    KAPGPD F A F               +K+  R    L  +N T I 
Sbjct: 534 KEITAAVFAMPRNKAPGPDSFTAEFFWESWFVVKDSTIAAVKEFFRTGHLLKRFNATAIT 593