Lag0006136 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0006136
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionReverse transcriptase domain-containing protein
Locationchr6: 38030236 .. 38030812 (+)
RNA-Seq ExpressionLag0006136
SyntenyLag0006136
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAGCCTAGCTGAAAATCTCGATGAAGAAGATCGTGGGAAAGCTACAAGCATCATTTGGAGTATTTGGAGTCACAGAAATTTGATAAAGAAAAATTCGGATAAGCTATATGGGAAGGAAGAAAGTACCTGAGTGCTGTCAGCAAAGAACCTTCAAGATTTAAGAGCTCCTTGAGTTAGAGTTGGTCCCCCCTCCTCGAAATTTCTGGAAAATAAATACAGATGCATCATGGTCTGAAGAAAAAAGAACTGGGAGAATCGGATGGATTGCTCGTGACTTTTTGGGATCTTTTATCTTTGCCGGTTTCAAGAGGATGAAGAGGAAATGGTCGATTCTCAGTCTGGAATTGAAAGCAATTGAAGAAAGTCTAAGGAGCTTACTTGAAGAAATTGGAAATCACCTCCCAAGGATCATCATCGAATCTAATTCAACAAACGTGACCGTCATCCTCAACCATGATTCCGAAGTCTTCTCGGGGAGCAGGTCGGTGGCGGACGATGTGGAGTATGCGGCGGAGCTGATCGGAAGCGTCGAGTTCAGCTTCAGCCGGCGGGAATCCAACATCATTGTGCATTAG

mRNA sequence

ATGAGCCTAGCTGAAAATCTCGATGAAGAAGATCGTGGGAAAGCTACAAGCATCATTTGGAGTATTTGGAGTCACAGAAATTTGATAAAGAAAAATTCGGATAAGCTATATGGGAAGGAAGAAAATGCATCATGGTCTGAAGAAAAAAGAACTGGGAGAATCGGATGGATTGCTCGTGACTTTTTGGGATCTTTTATCTTTGCCGGTTTCAAGAGGATGAAGAGGAAATGGTCGATTCTCAGTCTGGAATTGAAAGCAATTGAAGAAAGTCTAAGGAGCTTACTTGAAGAAATTGGAAATCACCTCCCAAGGATCATCATCGAATCTAATTCAACAAACGTGACCGTCATCCTCAACCATGATTCCGAAGTCTTCTCGGGGAGCAGGTCGGTGGCGGACGATGTGGAGTATGCGGCGGAGCTGATCGGAAGCGTCGAGTTCAGCTTCAGCCGGCGGGAATCCAACATCATTGTGCATTAG

Coding sequence (CDS)

ATGAGCCTAGCTGAAAATCTCGATGAAGAAGATCGTGGGAAAGCTACAAGCATCATTTGGAGTATTTGGAGTCACAGAAATTTGATAAAGAAAAATTCGGATAAGCTATATGGGAAGGAAGAAAATGCATCATGGTCTGAAGAAAAAAGAACTGGGAGAATCGGATGGATTGCTCGTGACTTTTTGGGATCTTTTATCTTTGCCGGTTTCAAGAGGATGAAGAGGAAATGGTCGATTCTCAGTCTGGAATTGAAAGCAATTGAAGAAAGTCTAAGGAGCTTACTTGAAGAAATTGGAAATCACCTCCCAAGGATCATCATCGAATCTAATTCAACAAACGTGACCGTCATCCTCAACCATGATTCCGAAGTCTTCTCGGGGAGCAGGTCGGTGGCGGACGATGTGGAGTATGCGGCGGAGCTGATCGGAAGCGTCGAGTTCAGCTTCAGCCGGCGGGAATCCAACATCATTGTGCATTAG

Protein sequence

MSLAENLDEEDRGKATSIIWSIWSHRNLIKKNSDKLYGKEENASWSEEKRTGRIGWIARDFLGSFIFAGFKRMKRKWSILSLELKAIEESLRSLLEEIGNHLPRIIIESNSTNVTVILNHDSEVFSGSRSVADDVEYAAELIGSVEFSFSRRESNIIVH
Homology
BLAST of Lag0006136 vs. NCBI nr
Match: XP_013694754.1 (uncharacterized protein LOC106398790 [Brassica napus])

HSP 1 Score: 55.5 bits (132), Expect = 5.0e-04
Identity = 31/118 (26.27%), Postives = 61/118 (51.69%), Query Frame = 0

Query: 42  NASWSEEKRTGRIGWIARDFLGSFIFAGFKRMKRKWSILSLELKAIEESLRSLLEEIGNH 101
           +A+W +E   G +GW+ RD  G  ++AG KR+    S+   E++A+  ++R+L    G  
Sbjct: 150 DATWMQETEVGGVGWVLRDHNGDMLWAGAKRLPDMGSVHETEMEALRWAIRTL---AGFG 209

Query: 102 LPRIIIESNSTNVTVILNHDSEVFSGSRSVADDVEYAAELIGSVEFSFSRRESNIIVH 160
              +  ES+S  +  +LN++ E++   + +   +  +   +  VE ++  R  N I H
Sbjct: 210 YRNVTFESDSQVLVRMLNNEEEIWPRVKPMIQAISSSPSGMEKVEVAYYPRSGNKIAH 264

BLAST of Lag0006136 vs. NCBI nr
Match: CAB4291403.1 (unnamed protein product [Prunus armeniaca])

HSP 1 Score: 55.5 bits (132), Expect = 5.0e-04
Identity = 43/133 (32.33%), Postives = 61/133 (45.86%), Query Frame = 0

Query: 28  LIKKNSDKLYGKEENASWSEEKRTGRIGWIARDFLGSFIFAGFKRMKRKWSILSLELKAI 87
           L+K NSD        A+WS ++  G +GW+ RD  G  + AG +      S L +EL AI
Sbjct: 296 LLKVNSD--------AAWSAQRMGGGVGWVIRDSFGLLLCAGGQGDLHGSSALMMELLAI 355

Query: 88  EESLRSLLEEIGN-HLPRIIIESNSTNVTVILNHDSEVFSGSRSVADDVEYAAELIGSVE 147
               R  L E  N HL  II+ES+S    ++LN  + V S    +  D+      +  V 
Sbjct: 356 ----RHALSECANFHLTDIIVESDSQKGILMLNGRATVDSDLEGIVFDIRQLVASLSRVS 415

Query: 148 FSFSRRESNIIVH 160
           F F+    N   H
Sbjct: 416 FVFAPSSCNQATH 416

BLAST of Lag0006136 vs. NCBI nr
Match: CAB4274760.1 (unnamed protein product [Prunus armeniaca])

HSP 1 Score: 55.1 bits (131), Expect = 6.5e-04
Identity = 38/119 (31.93%), Postives = 57/119 (47.90%), Query Frame = 0

Query: 42   NASWSEEKRTGRIGWIARDFLGSFIFAGFKRMKRKWSILSLELKAIEESLRSLLEEIGN- 101
            +A+WS ++  G +GW+ RD  G  + AG +      S + +EL AI    R  L E  N 
Sbjct: 1140 DAAWSAQRMGGGVGWVIRDSFGLLLCAGGQGDLHGSSAIMMELLAI----RHALSECANF 1199

Query: 102  HLPRIIIESNSTNVTVILNHDSEVFSGSRSVADDVEYAAELIGSVEFSFSRRESNIIVH 160
            HL  II+ES+S    ++LN  + V S    +  D+      +  V F F+ R  N   H
Sbjct: 1200 HLTDIIVESDSQKGILMLNGRATVDSDLEGIVFDIRQLVASLSRVSFVFAPRSCNQAAH 1254

BLAST of Lag0006136 vs. NCBI nr
Match: CAB4274761.1 (unnamed protein product [Prunus armeniaca])

HSP 1 Score: 55.1 bits (131), Expect = 6.5e-04
Identity = 38/119 (31.93%), Postives = 57/119 (47.90%), Query Frame = 0

Query: 42  NASWSEEKRTGRIGWIARDFLGSFIFAGFKRMKRKWSILSLELKAIEESLRSLLEEIGN- 101
           +A+WS ++  G +GW+ RD  G  + AG +      S + +EL AI    R  L E  N 
Sbjct: 272 DAAWSAQRMGGGVGWVIRDSFGLLLCAGGQGDLHGSSAIMMELLAI----RHALSECANF 331

Query: 102 HLPRIIIESNSTNVTVILNHDSEVFSGSRSVADDVEYAAELIGSVEFSFSRRESNIIVH 160
           HL  II+ES+S    ++LN  + V S    +  D+      +  V F F+ R  N   H
Sbjct: 332 HLTDIIVESDSQKGILMLNGRATVDSDLEGIVFDIRQLVASLSRVSFVFAPRSCNQAAH 386

BLAST of Lag0006136 vs. NCBI nr
Match: CAB4303756.1 (unnamed protein product [Prunus armeniaca])

HSP 1 Score: 55.1 bits (131), Expect = 6.5e-04
Identity = 38/119 (31.93%), Postives = 57/119 (47.90%), Query Frame = 0

Query: 42  NASWSEEKRTGRIGWIARDFLGSFIFAGFKRMKRKWSILSLELKAIEESLRSLLEEIGN- 101
           +A+WS ++  G +GW+ RD  G  + AG +      S + +EL AI    R  L E  N 
Sbjct: 809 DAAWSAQRMGGGVGWVIRDSFGLLLCAGGQGDLHGSSAIMMELLAI----RHALSECANF 868

Query: 102 HLPRIIIESNSTNVTVILNHDSEVFSGSRSVADDVEYAAELIGSVEFSFSRRESNIIVH 160
           HL  II+ES+S    ++LN  + V S    +  D+      +  V F F+ R  N   H
Sbjct: 869 HLTDIIVESDSQKGILMLNGRATVDSDLEGIVFDIRQLVASLSRVSFVFAPRSCNQAAH 923

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_013694754.15.0e-0426.27uncharacterized protein LOC106398790 [Brassica napus][more]
CAB4291403.15.0e-0432.33unnamed protein product [Prunus armeniaca][more]
CAB4274760.16.5e-0431.93unnamed protein product [Prunus armeniaca][more]
CAB4274761.16.5e-0431.93unnamed protein product [Prunus armeniaca][more]
CAB4303756.16.5e-0431.93unnamed protein product [Prunus armeniaca][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 42..158
e-value: 1.0E-8
score: 35.0
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 43..159
e-value: 2.98035E-9
score: 50.0052

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0006136.1Lag0006136.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity