Lag0006037 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0006037
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionRNase H domain-containing protein
Locationchr6: 36360508 .. 36361196 (+)
RNA-Seq ExpressionLag0006037
SyntenyLag0006037
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGATATTGATATCACATGTCCTTGTTGTTTCAATTTCCCTGAATATACTGACCATTCTCTTTTTGAATGTTCTCGTGCTAAGGAGATTTGGAGAATTACTTGCAACAAAGTCTTCCTCGAGTCTGATTTCAATGGAAACATCGCAGATCGTTGGTTGAAGATCGGTTCCTCCTGCGCCCAGAAGGATCTGGAGCTTATTGCCATCACTTGTTGGGCAATTTGGTTGGACAGGAACAAATTAGTCCACGATGAAGCTCTCCCCCTTCTAATACGAGAAGCAAATGGATTTCTAATTACCTGACGAATTATATTGAAGCAAATTCGAGGAAGCTATCTAGCCCTCCTTCTTCTTCCCTACCATCCACCTCGATCAAATTCTGGTCTCCTCCCCCGGTGAATTTCTGGAAAGTCAATGTTGATGCGGTATGGCTCTCTTCCTCCCTTGAGCTAGGTATCGAAGCCATTCTCAGAAGGCCAAACAATGATATTGTGGGTGCTTCCTCGCGGTTTCTCAACATCTCCCTCGAAGGTCCAGCTGCTGAACTCGAAGCCATCAAAGAAGGCATCAATCTGGCCCTCTCCCTAGGATGTGATAGTCTCCTAGTGGAATCTGACAACCAATTGGCCATCAATTTTCTAAACAGAAAATCTGAAAATGTGGTGTACTTTAGAAGGCCTTCTCTGTAG

mRNA sequence

ATGGATATTGATATCACATGTCCTTGTTGTTTCAATTTCCCTGAATATACTGACCATTCTCTTTTTGAATGTTCTCGTGCTAAGGAGATTTGGAGAATTACTTGCAACAAAGTCTTCCTCGAGTCTGATTTCAATGGAAACATCGCAGATCGTTGGTTGAAGATCGGTTCCTCCTGCGCCCAGAAGGATCTGGAGCTTATTGCCATCACTTCAAATTCGAGGAAGCTATCTAGCCCTCCTTCTTCTTCCCTACCATCCACCTCGATCAAATTCTGGTCTCCTCCCCCGGTGAATTTCTGGAAAGTCAATGTTGATGCGGTATGGCTCTCTTCCTCCCTTGAGCTAGGTATCGAAGCCATTCTCAGAAGGCCAAACAATGATATTGTGGGTGCTTCCTCGCGGTTTCTCAACATCTCCCTCGAAGGTCCAGCTGCTGAACTCGAAGCCATCAAAGAAGGCATCAATCTGGCCCTCTCCCTAGGATGTGATAGTCTCCTAGTGGAATCTGACAACCAATTGGCCATCAATTTTCTAAACAGAAAATCTGAAAATGTGGTGTACTTTAGAAGGCCTTCTCTGTAG

Coding sequence (CDS)

ATGGATATTGATATCACATGTCCTTGTTGTTTCAATTTCCCTGAATATACTGACCATTCTCTTTTTGAATGTTCTCGTGCTAAGGAGATTTGGAGAATTACTTGCAACAAAGTCTTCCTCGAGTCTGATTTCAATGGAAACATCGCAGATCGTTGGTTGAAGATCGGTTCCTCCTGCGCCCAGAAGGATCTGGAGCTTATTGCCATCACTTCAAATTCGAGGAAGCTATCTAGCCCTCCTTCTTCTTCCCTACCATCCACCTCGATCAAATTCTGGTCTCCTCCCCCGGTGAATTTCTGGAAAGTCAATGTTGATGCGGTATGGCTCTCTTCCTCCCTTGAGCTAGGTATCGAAGCCATTCTCAGAAGGCCAAACAATGATATTGTGGGTGCTTCCTCGCGGTTTCTCAACATCTCCCTCGAAGGTCCAGCTGCTGAACTCGAAGCCATCAAAGAAGGCATCAATCTGGCCCTCTCCCTAGGATGTGATAGTCTCCTAGTGGAATCTGACAACCAATTGGCCATCAATTTTCTAAACAGAAAATCTGAAAATGTGGTGTACTTTAGAAGGCCTTCTCTGTAG

Protein sequence

MDIDITCPCCFNFPEYTDHSLFECSRAKEIWRITCNKVFLESDFNGNIADRWLKIGSSCAQKDLELIAITSNSRKLSSPPSSSLPSTSIKFWSPPPVNFWKVNVDAVWLSSSLELGIEAILRRPNNDIVGASSRFLNISLEGPAAELEAIKEGINLALSLGCDSLLVESDNQLAINFLNRKSENVVYFRRPSL
Homology
BLAST of Lag0006037 vs. NCBI nr
Match: XP_038687446.1 (uncharacterized protein LOC119986824 [Tripterygium wilfordii])

HSP 1 Score: 90.9 bits (224), Expect = 1.3e-14
Identity = 68/194 (35.05%), Postives = 99/194 (51.03%), Query Frame = 0

Query: 7   CPCCFNFP--EYTDHSLFECSRAKEIW----RITCNKVFLESDFNGNIADRWLKIGSSCA 66
           C CC NFP  E  +H L E S A ++W    ++T  ++       G I D+ +++   C 
Sbjct: 128 CDCCTNFPHVETVEHVLVEGSIACQVWFDQTQLTRGQII------GEIIDQ-IRVQFQCK 187

Query: 67  QKDLELIAITSNSRKLSSPP--SSSLPSTSIKFWSPPPVNFWKVNVDAVWLSSSLELGIE 126
            K L +    SN  KL   P  +SS    SI +W+PPP +   +N D   L++  + G  
Sbjct: 188 VKTLHMELRDSNVSKLFCIPMRASSKGKVSILYWNPPPHDVTIINCDGSALNNPRDSGGG 247

Query: 127 AILRRPNNDIVGASSRFLNISLEGPAAELEAIKEGINLALSLGCDSLLVESDNQLAINFL 186
            ++RR +   V A S F   S     AE+ AI EGINLA SLG  +LLVESD+ + IN L
Sbjct: 248 GVIRRSSGIFVAAFSNFYG-SNSITFAEMMAIHEGINLAASLGLSNLLVESDSLIVINIL 307

Query: 187 NRKSE---NVVYFR 190
           +       N++Y +
Sbjct: 308 SESMSRPWNLIYLK 313

BLAST of Lag0006037 vs. NCBI nr
Match: KAF7838458.1 (reverse transcriptase [Senna tora])

HSP 1 Score: 82.0 bits (201), Expect = 6.1e-12
Identity = 59/192 (30.73%), Postives = 90/192 (46.88%), Query Frame = 0

Query: 3   IDITCPCCFNFPEYTDHSLFECSRAKEIWRI-TCNKVFLESDFNGNIADRWLK------- 62
           ++I CP C    E  DH    C   +++W +   N   L+S   G+   +WLK       
Sbjct: 513 LNIVCPVCSVGVESLDHLFASCFETRKVWEVMNVNPTILDS---GSCFQKWLKLNAGNKK 572

Query: 63  -----IGSSCAQKDLELIAITSNSRKLSSPPSSSLPSTSIKFWSPPPVNFWKVNVDAVWL 122
                +G     K +E + + S        P  ++ +  I  W PPP+ +WK+N D    
Sbjct: 573 FDCLRVGRRAVCKAVEFVYLNS-----KYSPERAVETVFIG-WQPPPLGWWKLNTDGSCQ 632

Query: 123 SSSLELGIEAILRRPNNDIVGASSRFLNISLEGPA--AELEAIKEGINLALSLGCDSLLV 180
           ++   +G   I+R  N + +    +FL    EG    AEL AI EG+NLA  L CD L+V
Sbjct: 633 NNL--IGGGGIIRDANGNWIHGFKKFLG---EGDCLLAELWAIVEGVNLAKHLHCDKLIV 690

BLAST of Lag0006037 vs. NCBI nr
Match: ONI15824.1 (hypothetical protein PRUPE_3G063400 [Prunus persica])

HSP 1 Score: 78.2 bits (191), Expect = 8.8e-11
Identity = 53/185 (28.65%), Postives = 92/185 (49.73%), Query Frame = 0

Query: 4   DITCPCCFNFPEYTDHSLFECSRAKEIWRITCNKVFLESDFNGNIADRWLKIGSSCAQKD 63
           D  CP C    E   H ++ C  A+++W+    KV     F G     +     +C++K+
Sbjct: 142 DDACPICIVNSENLIHVVWSCPGAQKVWK----KVRFMEVFKGLQLSTYGDFFEACSKKE 201

Query: 64  LEL----IAITSNSRKLSSPPSSSLPSTSIKFWSPPPVNFWKVNVDAVWLSSSLELGIEA 123
             +    + +T     L    S+ +   + K WS PPV  +K+NVDA ++  +   GI A
Sbjct: 202 EMIFNGAVHLTEEYGDLMRRESNIVIEKASK-WSHPPVGKFKLNVDAAYIPDTGVGGIGA 261

Query: 124 ILRRPNNDIVGASSRFLNISLEGPAAELEAIKEGINLALSLGCDSLLVESDNQLAINFLN 183
           ++R    +++ A++  L+ +     AE+ A++ G+N A   G  S+LVESD+Q  +N LN
Sbjct: 262 VIRNEKGEVMVATALPLHTATSPKHAEIMALQFGLNFAWDAGFSSILVESDSQTVVNNLN 321

Query: 184 RKSEN 185
              E+
Sbjct: 322 TDEES 321

BLAST of Lag0006037 vs. NCBI nr
Match: XP_024033483.1 (uncharacterized protein LOC112095606 [Citrus clementina])

HSP 1 Score: 75.5 bits (184), Expect = 5.7e-10
Identity = 48/168 (28.57%), Postives = 83/168 (49.40%), Query Frame = 0

Query: 19  HSLFECSRAKEIWRITCNKVFLESDFNGNIADRWLKIGSSCAQKDL--ELIAITSNSRKL 78
           H+L EC  A++IW++T     LE+D      +  L  G     + L  +  A+    +++
Sbjct: 470 HALVECKAARKIWKLT----LLENDIMQTAGNEMLFKGKRENPQALVAKAEAVMEAYKRI 529

Query: 79  SSPPSSSLPSTSI---KFWSPPPVNFWKVNVDAVWLSSSLELGIEAILRRPNNDIVGASS 138
             P  + + S  +   + W+PP   F KVNVDA   S     G+ AI+R    +++ A+ 
Sbjct: 530 QKPEQARMESQQVQAQQVWNPPQSGFVKVNVDAATSSEKHLAGLGAIIRDDRGNVIAAAI 589

Query: 139 RFLNISLEGPAAELEAIKEGINLALSLGCDSLLVESDNQLAINFLNRK 182
           +      +   AE EA++ G+ +A +    +L+VESD Q  +N +N K
Sbjct: 590 KISKFHGDVSFAEAEALEWGLQIARNASTKALIVESDAQSVVNLVNNK 633

BLAST of Lag0006037 vs. NCBI nr
Match: OMP06477.1 (hypothetical protein CCACVL1_01552 [Corchorus capsularis])

HSP 1 Score: 75.1 bits (183), Expect = 7.4e-10
Identity = 56/213 (26.29%), Postives = 89/213 (41.78%), Query Frame = 0

Query: 1    MDIDITCPCCFNFPEYTDHSLFECSRAKEIWRITCNKVFL---ESDFNGNIADRWLKIGS 60
            M+I  TC  C        H  F C  +K +W   C  V     + D NGN  + +L+   
Sbjct: 1128 MEIAGTCEVCGGEESADAHVFFNCHLSKLVWEDACPWVLSCIEQWDLNGNFWEFFLEKAK 1187

Query: 61   SCAQKD---LELIAITSNSRKLSSPPSSSLPSTSIK------------------------ 120
            +  Q D     L  +  NS +       S+P+  ++                        
Sbjct: 1188 AIGQFDRVCTILWLLWGNSNRALYEAFCSMPNAIVRAATRILDQVCAATSRIGEILMGQS 1247

Query: 121  ---FWSPPPVNFWKVNVDAVWLSSSLELGIEAILRRPNNDIVGASSRFLNISLEGPAAEL 180
                W PPP+   K+N DA + + + E G+  ++R    +++ + SR L    +   AE+
Sbjct: 1248 RQIAWMPPPLGVMKINTDASFSTENGEAGLGVVIRDDVGNVIASGSRRLYFIADSLYAEV 1307

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_038687446.11.3e-1435.05uncharacterized protein LOC119986824 [Tripterygium wilfordii][more]
KAF7838458.16.1e-1230.73reverse transcriptase [Senna tora][more]
ONI15824.18.8e-1128.65hypothetical protein PRUPE_3G063400 [Prunus persica][more]
XP_024033483.15.7e-1028.57uncharacterized protein LOC112095606 [Citrus clementina][more]
OMP06477.17.4e-1026.29hypothetical protein CCACVL1_01552 [Corchorus capsularis][more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR036397Ribonuclease H superfamilyGENE3D3.30.420.10coord: 99..189
e-value: 1.6E-7
score: 33.4
IPR002156Ribonuclease H domainPFAMPF13456RVT_3coord: 103..183
e-value: 4.1E-10
score: 39.5
NoneNo IPR availablePANTHERPTHR33033POLYNUCLEOTIDYL TRANSFERASE, RIBONUCLEASE H-LIKE SUPERFAMILY PROTEIN-RELATEDcoord: 77..178
IPR044730Ribonuclease H-like domain, plant typeCDDcd06222RNase_H_likecoord: 102..183
e-value: 2.28264E-9
score: 51.1608
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 98..181

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0006037.1Lag0006037.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0004523 RNA-DNA hybrid ribonuclease activity