Lag0005960 (gene) Sponge gourd (AG‐4) v1

Overview
NameLag0005960
Typegene
OrganismLuffa acutangula (Sponge gourd (AG‐4) v1)
DescriptionEnzymatic polyprotein
Locationchr6: 34572516 .. 34574400 (+)
RNA-Seq ExpressionLag0005960
SyntenyLag0005960
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGAACCATTACAGACGACCATCTCCTCCAGATCTGGGATGGGATGATCTCGCTCCTGATCATAGGACCTTCGATCCGAAATCCCTAACGACATGGAATATCGACGGCTGTTCAGAAGGTCAAATTGTTAGACTGTTTGAAGAAATGTTTGCCGCAGCAATAGTCTATACTCAAAAATTTAGTCAAAAGGATACAACTCAAATCCTAGTTTCGGGATTCACAGGTATTCTAAGAAGTTGGTGGCACAACCACCTTACAGCTGAGAATCGAGAGTTGATTCTCAATCACCAAATAGAAGAAATAGCAAAAGGCGTCGATGGTATCGAATATACCACGATGGCTCCTAATGCAGTCAATGCCCTTATCTATACGACATTAAAGAACTTTGTAGGACACACGACCCTATACAGTGATCAAGCAACAGCTGCTCTGCTAAGCCTAAAATGTCCTAAGATTAGTGACTTCAAATGGTATAAAGACACTTTTCTTATGTGTCTTTACATTCTCACTACATGCCATGATGTCCTATGGAAACACAAATATGTTGAAGGACTTCCAAGGTATGTCAAAGAAAAGTTCCATTCCACTATGATAGCCAATCAAGGTGGAGCAGAAGTCAATTGGGCAAGTATCACATATGGTGACATCAATGCCACTATTCAATCAGTATGTCTGGAGATTTGTCAAAACCAGAAACATACAGCCAAAGTAGTCAGACTCTGACTACATAAAAGAATTAGGAAATTTCTGCAGCCAGTATGGCCTCGAAGATCTTCCTCCTTCCAAAAGAAAGAATGGCTCTTTCAAAAAGACCTTCAAAAAGCAAAAGAATATTATTTCTGAAGCTCCTCGCAGACGACAGAGGTTCAAAAGCAAAAAGAAGCAATCTACAAAAGAAACCGTATGCTTCAAATGCAAAAAACCAGGACACTATGCCAATCGGTGCCCATTGGTCAAGAAAGTTAACAAATTAGAGATCGACGAAGATACTAAACAGTCTCTTCTTCGAATCTTAAACGATTATTCTTCAGAGACAGACTCCTCCTCCTCCTCCGATGAAGACTTGGGAAGAATAAATGAAATTCTCGAAGAATCCTCAGGAGAATCTTCATTTTCTAGTGAAAAGTCTGAAGATGACGGTGCTATTCCCTGTAGTGGATGCATCAATGTTCTGACTTCTTATCAAAAAGATCTTTTGGATCTAATTGATGAAATCCCTGATAAGGAAGCTCAAAAATCAATTCTCCTAAAGCTTCGATCAAGTTTAAAAGCTCAGGAGGTTCGACCCAGAAATCCGGTAGATTTCTCTTTTCAGAGTATCTTGAACCAGCTCCAAAGTGAAGGTACGACTTCAGTCAAAATTCCAGATATGCAACAAGAAATCAAATCCTTGAAGAAGGAGGTTGCAGAAAACAAAACAAGAATTACCAATCTTGAGGAAGCCTTCTATCTTATCCAAGAAAAGACAATCTTCCAAGATATTCAAAAGGGGCCTAGCAATGAGTCAGGTCCATCTTCTCAAAATGAGGATGGCATAAATGCCATCAGCAAAGTCTTCAATAAAAAATGGTTATCCAATATCACACTGAAGATTCAAGACTCTAAGCTCGAAACTATTGCTCTAATAGATTCCGGAGCCGATCAAAATGTCATTCGCGAAGGATTGATACCAACAAGATATTGCGAAATGACAAAACAAGATCTTAGAGGAGCAGGCAACTATCCACTAAAGATCAGTTACAAATTATCCAACGTTCATATCTGCAATGAAGAAGTGTGTTTCGTAAGCACATTTCTTATTGTAAAGGATCTGACGGAAGAAGTAATTCTGGGTACTCCTTTCTTAACCCAGTTATATCCATTCATAGTTTCTGAGAAATGA

mRNA sequence

ATGAAGAACCATTACAGACGACCATCTCCTCCAGATCTGGGATGGGATGATCTCGCTCCTGATCATAGGACCTTCGATCCGAAATCCCTAACGACATGGAATATCGACGGCTGTTCAGAAGGTCAAATTGTTAGACTGTTTGAAGAAATGTTTGCCGCAGCAATAGTCTATACTCAAAAATTTAGTCAAAAGGATACAACTCAAATCCTAGTTTCGGGATTCACAGGTATTCTAAGAAGTTGGTGGCACAACCACCTTACAGCTGAGAATCGAGAGTTGATTCTCAATCACCAAATAGAAGAAATAGCAAAAGGCGTCGATGGTATCGAATATACCACGATGGCTCCTAATGCAGTCAATGCCCTTATCTATACGACATTAAAGAACTTTGTAGGACACACGACCCTATACAGTGATCAAGCAACAGCTGCTCTGCTAAGCCTAAAATGTCCTAAGATTAGTGACTTCAAATGGTATAAAGACACTTTTCTTATGTGTCTTTACATTCTCACTACATGCCATGATGTCCTATGGAAACACAAATATGTTGAAGGACTTCCAAGCCAGTATGGCCTCGAAGATCTTCCTCCTTCCAAAAGAAAGAATGGCTCTTTCAAAAAGACCTTCAAAAAGCAAAAGAATATTATTTCTGAAGCTCCTCGCAGACGACAGAGGTTCAAAAGCAAAAAGAAGCAATCTACAAAAGAAACCGTATGCTTCAAATGCAAAAAACCAGGACACTATGCCAATCGGTGCCCATTGGTCAAGAAAGTTAACAAATTAGAGATCGACGAAGATACTAAACAGTCTCTTCTTCGAATCTTAAACGATTATTCTTCAGAGACAGACTCCTCCTCCTCCTCCGATGAAGACTTGGGAAGAATAAATGAAATTCTCGAAGAATCCTCAGGAGAATCTTCATTTTCTAGTGAAAAGTCTGAAGATGACGGTGCTATTCCCTGTAGTGGATGCATCAATGTTCTGACTTCTTATCAAAAAGATCTTTTGGATCTAATTGATGAAATCCCTGATAAGGAAGCTCAAAAATCAATTCTCCTAAAGCTTCGATCAAGTTTAAAAGCTCAGGAGGTTCGACCCAGAAATCCGGTAGATTTCTCTTTTCAGAGTATCTTGAACCAGCTCCAAAGTGAAGGTACGACTTCAGTCAAAATTCCAGATATGCAACAAGAAATCAAATCCTTGAAGAAGGAGGTTGCAGAAAACAAAACAAGAATTACCAATCTTGAGGAAGCCTTCTATCTTATCCAAGAAAAGACAATCTTCCAAGATATTCAAAAGGGGCCTAGCAATGAGTCAGGTCCATCTTCTCAAAATGAGGATGGCATAAATGCCATCAGCAAAGTCTTCAATAAAAAATGGTTATCCAATATCACACTGAAGATTCAAGACTCTAAGCTCGAAACTATTGCTCTAATAGATTCCGGAGCCGATCAAAATGTCATTCGCGAAGGATTGATACCAACAAGATATTGCGAAATGACAAAACAAGATCTTAGAGGAGCAGGCAACTATCCACTAAAGATCAGTTACAAATTATCCAACGTTCATATCTGCAATGAAGAAGTGTGTTTCGTAAGCACATTTCTTATTGTAAAGGATCTGACGGAAGAAGTAATTCTGGGTACTCCTTTCTTAACCCAGTTATATCCATTCATAGTTTCTGAGAAATGA

Coding sequence (CDS)

ATGAAGAACCATTACAGACGACCATCTCCTCCAGATCTGGGATGGGATGATCTCGCTCCTGATCATAGGACCTTCGATCCGAAATCCCTAACGACATGGAATATCGACGGCTGTTCAGAAGGTCAAATTGTTAGACTGTTTGAAGAAATGTTTGCCGCAGCAATAGTCTATACTCAAAAATTTAGTCAAAAGGATACAACTCAAATCCTAGTTTCGGGATTCACAGGTATTCTAAGAAGTTGGTGGCACAACCACCTTACAGCTGAGAATCGAGAGTTGATTCTCAATCACCAAATAGAAGAAATAGCAAAAGGCGTCGATGGTATCGAATATACCACGATGGCTCCTAATGCAGTCAATGCCCTTATCTATACGACATTAAAGAACTTTGTAGGACACACGACCCTATACAGTGATCAAGCAACAGCTGCTCTGCTAAGCCTAAAATGTCCTAAGATTAGTGACTTCAAATGGTATAAAGACACTTTTCTTATGTGTCTTTACATTCTCACTACATGCCATGATGTCCTATGGAAACACAAATATGTTGAAGGACTTCCAAGCCAGTATGGCCTCGAAGATCTTCCTCCTTCCAAAAGAAAGAATGGCTCTTTCAAAAAGACCTTCAAAAAGCAAAAGAATATTATTTCTGAAGCTCCTCGCAGACGACAGAGGTTCAAAAGCAAAAAGAAGCAATCTACAAAAGAAACCGTATGCTTCAAATGCAAAAAACCAGGACACTATGCCAATCGGTGCCCATTGGTCAAGAAAGTTAACAAATTAGAGATCGACGAAGATACTAAACAGTCTCTTCTTCGAATCTTAAACGATTATTCTTCAGAGACAGACTCCTCCTCCTCCTCCGATGAAGACTTGGGAAGAATAAATGAAATTCTCGAAGAATCCTCAGGAGAATCTTCATTTTCTAGTGAAAAGTCTGAAGATGACGGTGCTATTCCCTGTAGTGGATGCATCAATGTTCTGACTTCTTATCAAAAAGATCTTTTGGATCTAATTGATGAAATCCCTGATAAGGAAGCTCAAAAATCAATTCTCCTAAAGCTTCGATCAAGTTTAAAAGCTCAGGAGGTTCGACCCAGAAATCCGGTAGATTTCTCTTTTCAGAGTATCTTGAACCAGCTCCAAAGTGAAGGTACGACTTCAGTCAAAATTCCAGATATGCAACAAGAAATCAAATCCTTGAAGAAGGAGGTTGCAGAAAACAAAACAAGAATTACCAATCTTGAGGAAGCCTTCTATCTTATCCAAGAAAAGACAATCTTCCAAGATATTCAAAAGGGGCCTAGCAATGAGTCAGGTCCATCTTCTCAAAATGAGGATGGCATAAATGCCATCAGCAAAGTCTTCAATAAAAAATGGTTATCCAATATCACACTGAAGATTCAAGACTCTAAGCTCGAAACTATTGCTCTAATAGATTCCGGAGCCGATCAAAATGTCATTCGCGAAGGATTGATACCAACAAGATATTGCGAAATGACAAAACAAGATCTTAGAGGAGCAGGCAACTATCCACTAAAGATCAGTTACAAATTATCCAACGTTCATATCTGCAATGAAGAAGTGTGTTTCGTAAGCACATTTCTTATTGTAAAGGATCTGACGGAAGAAGTAATTCTGGGTACTCCTTTCTTAACCCAGTTATATCCATTCATAGTTTCTGAGAAATGA

Protein sequence

MKNHYRRPSPPDLGWDDLAPDHRTFDPKSLTTWNIDGCSEGQIVRLFEEMFAAAIVYTQKFSQKDTTQILVSGFTGILRSWWHNHLTAENRELILNHQIEEIAKGVDGIEYTTMAPNAVNALIYTTLKNFVGHTTLYSDQATAALLSLKCPKISDFKWYKDTFLMCLYILTTCHDVLWKHKYVEGLPSQYGLEDLPPSKRKNGSFKKTFKKQKNIISEAPRRRQRFKSKKKQSTKETVCFKCKKPGHYANRCPLVKKVNKLEIDEDTKQSLLRILNDYSSETDSSSSSDEDLGRINEILEESSGESSFSSEKSEDDGAIPCSGCINVLTSYQKDLLDLIDEIPDKEAQKSILLKLRSSLKAQEVRPRNPVDFSFQSILNQLQSEGTTSVKIPDMQQEIKSLKKEVAENKTRITNLEEAFYLIQEKTIFQDIQKGPSNESGPSSQNEDGINAISKVFNKKWLSNITLKIQDSKLETIALIDSGADQNVIREGLIPTRYCEMTKQDLRGAGNYPLKISYKLSNVHICNEEVCFVSTFLIVKDLTEEVILGTPFLTQLYPFIVSEK
Homology
BLAST of Lag0005960 vs. NCBI nr
Match: KAA0056776.1 (Enzymatic polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 491.5 bits (1264), Expect = 9.7e-135
Identity = 294/661 (44.48%), Postives = 388/661 (58.70%), Query Frame = 0

Query: 1    MKNHYRRPSPPDLGWDDLAPDHRTFDPKSLTTWNIDGCSEGQIVRLFEEMFAAAIVYTQK 60
            MKNHY +PSPPDLGWDDL  + RT+D +SL TWNIDG SE Q++  F+EM  AA  Y+ K
Sbjct: 617  MKNHYPQPSPPDLGWDDLHHEKRTYDGQSLITWNIDGYSEAQMMNTFQEMLLAATAYSTK 676

Query: 61   FSQKDTTQILVSGFTGILRSWWHNHLTAENRELILNHQIEEIAKGVDGIEYTTMAPNAVN 120
             S  +T QIL+ GF G LRSWWHN LT ++R+ IL      +             P+ VN
Sbjct: 677  KSTYETAQILILGFNGNLRSWWHNLLTEQDRQRILTATRTVVKTENTSTPIQVEEPDMVN 736

Query: 121  ALIYTTLKNFVGHTTLYSDQATAALLSLKCPKISDFKWYKDTFLMCLYILTTCHDVLWKH 180
             L+YT  K+F+G T ++ + AT ALL LKC K+S +KWYKDTF+  LY LTTC   +WK 
Sbjct: 737  QLLYTMTKHFIGSTQIHLNLATEALLGLKCHKMSRYKWYKDTFMARLYTLTTCGADIWKQ 796

Query: 181  KYVEGLP----------------------------------------------------- 240
            K+VEGLP                                                     
Sbjct: 797  KFVEGLPHYISQKFYQTMTANSVNQQIDWANLTYGDISSTVQMICVNLCTENKHTTKVIK 856

Query: 241  ------------SQYGLEDLP----PSKRKNGSFKKTFKKQKNIISEAPRRRQRF----K 300
                         QYGL   P      K+K  S KK F+K K    E+PRRR+R     K
Sbjct: 857  DSDYRKELGTFCKQYGLSQGPKEEKKKKKKRYSSKKFFRKSKAKDQESPRRRRRHYNKGK 916

Query: 301  SKKKQSTK-ETVCFKCKKPGHYANRCPLVKKVNKLEIDEDTKQSLL-RILNDYSSETDSS 360
            SKK  S+K  T+CFKC + GHYANRCPL  K+N + IDE+TKQSLL  I +D  + + + 
Sbjct: 917  SKKGYSSKTHTICFKCNQKGHYANRCPLKDKINAMTIDEETKQSLLYAIRSDDDTTSQTE 976

Query: 361  SSSDEDLGRINEILEE-SSGESSF--SSEKSEDDGAIP--------CSGCINVLTSYQKD 420
            SSS+ED   IN + EE SS E  F   S+ S+D+GAIP        CSG INV+T  Q+ 
Sbjct: 977  SSSEEDY--INILQEEGSSSEEEFYSQSDSSDDEGAIPCTGRCAGKCSGHINVITKDQET 1036

Query: 421  LLDLIDEIPDKEAQKSILLKLRSSLKAQEVRP--RNPVDFSFQSILNQLQSEGTTSVKIP 480
            L DLI++IPD+EA+++ LLKL+ SL+ Q  +   +NP+ +S+Q ILN+++ E    +++ 
Sbjct: 1037 LFDLIEQIPDEEAKRTCLLKLKQSLEEQAPQKAIQNPIMYSYQDILNRVKGEAKMPIQVE 1096

Query: 481  DMQQEIKSLKKEVAENKTRITNLEEAFYLIQEKTIFQDIQKGPSNESGPSSQNEDG---- 540
            D+  E+K+LK+EVAENK R+  LE AF        FQ  Q      +    +   G    
Sbjct: 1097 DLHHEVKTLKREVAENKQRLIYLETAF------QAFQGSQASKEESTSDFERKSAGKALL 1156

Query: 541  ------INAISKVFNKKWLSNITLKIQDSKLETIALIDSGADQNVIREGLIPTRYCEMTK 564
                  IN+ISK+ N+KW+S I  K++D +LE +ALIDSGADQNVI+EGL+P+RY E TK
Sbjct: 1157 IEEIGTINSISKIQNQKWMSKIVFKVKDFQLEALALIDSGADQNVIQEGLVPSRYFEKTK 1216

BLAST of Lag0005960 vs. NCBI nr
Match: TYJ98087.1 (Enzymatic polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 489.6 bits (1259), Expect = 3.7e-134
Identity = 293/660 (44.39%), Postives = 390/660 (59.09%), Query Frame = 0

Query: 1    MKNHYRRPSPPDLGWDDLAPDHRTFDPKSLTTWNIDGCSEGQIVRLFEEMFAAAIVYTQK 60
            MKNHY +PSPPDLGWDDL  + RT+D +SL TWNIDG SE Q++  F+EM  AA  Y+ K
Sbjct: 616  MKNHYPQPSPPDLGWDDLHHEKRTYDGQSLITWNIDGYSEAQMMNTFQEMLLAATAYSTK 675

Query: 61   FSQKDTTQILVSGFTGILRSWWHNHLTAENRELILNHQIEEIAKGVDGIEYTTMAPNAVN 120
             S  +T QIL+ GF G LRSWWHN LT ++R+ IL      +             P+ VN
Sbjct: 676  KSTYETAQILILGFNGNLRSWWHNLLTEQDRQRILTATRTVVKTENTSTPIQVEEPDMVN 735

Query: 121  ALIYTTLKNFVGHTTLYSDQATAALLSLKCPKISDFKWYKDTFLMCLYILTTCHDVLWKH 180
             L+YT  K+F+G T ++ + AT ALL LK  K+S +KWYKDTF+  LY LTTC   +WK 
Sbjct: 736  QLLYTMTKHFIGSTQIHLNLATEALLGLKYHKMSRYKWYKDTFMARLYTLTTCGADIWKQ 795

Query: 181  KYVEGLP----------------------------------------------------- 240
            K+VEGLP                                                     
Sbjct: 796  KFVEGLPHYISQKFYQTMTANSVNQQIDWANLTYGDISSTVQMINVNLCTENKHTTKVIK 855

Query: 241  ------------SQYGLEDLP----PSKRKNGSFKKTFKKQKNIISEAPRRRQ----RFK 300
                         QYGL   P      K+K  S KK F+K K    E+P+RR+    + K
Sbjct: 856  DSDYRKELGTFCKQYGLSQGPKEEKKKKKKRYSSKKFFRKGKVKDQESPQRRRHHYYKGK 915

Query: 301  SKKKQSTK-ETVCFKCKKPGHYANRCPLVKKVNKLEIDEDTKQSLL---RILNDYSSETD 360
             KKK S+K  T+CFKC + GHYANRCPL  K+N L IDE+TKQSLL   R+ +D SS+T+
Sbjct: 916  GKKKYSSKTNTICFKCNQKGHYANRCPLKDKINALTIDEETKQSLLYAIRMDDDTSSQTE 975

Query: 361  SSSSSDEDLGRINEILEE-SSGESSF--SSEKSEDDGAIP--------CSGCINVLTSYQ 420
            SSS  D     IN + EE SS E  F   S+ S+D+GAIP        CSG INV+T  Q
Sbjct: 976  SSSEED----YINILQEEGSSSEEEFYSQSDSSDDEGAIPCTGRCAGKCSGHINVITKDQ 1035

Query: 421  KDLLDLIDEIPDKEAQKSILLKLRSSLKAQEVRP--RNPVDFSFQSILNQLQSEGTTSVK 480
            + L  LI++IPD+EA+++ LLKL+ SL+ Q  +   +NP+ +S+Q ILN+++ E    ++
Sbjct: 1036 ETLFYLIEQIPDEEAKRTCLLKLKQSLEEQAPQKAIQNPIMYSYQDILNRVKGEAKMPIQ 1095

Query: 481  IPDMQQEIKSLKKEVAENKTRITNLEEAFYLIQEKTIFQDIQKGPSNE-------SGPSS 540
            + D+  E+K+LK+EVAENK R+  LE AF   QE  + ++  +   N+            
Sbjct: 1096 VEDLHHEVKTLKREVAENKQRLIYLENAFQAFQESQVLKENSETSRNDFERKIARKALLI 1155

Query: 541  QNEDGINAISKVFNKKWLSNITLKIQDSKLETIALIDSGADQNVIREGLIPTRYCEMTKQ 564
             +   IN+ISKV NKKW+S I  K++D +LET+ALIDSGADQNVI+EGL+P++Y E TK+
Sbjct: 1156 DDSGKINSISKVHNKKWMSKIVFKVKDFQLETLALIDSGADQNVIQEGLVPSKYFEKTKE 1215

BLAST of Lag0005960 vs. NCBI nr
Match: TYJ97599.1 (Enzymatic polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 489.6 bits (1259), Expect = 3.7e-134
Identity = 292/661 (44.18%), Postives = 388/661 (58.70%), Query Frame = 0

Query: 1    MKNHYRRPSPPDLGWDDLAPDHRTFDPKSLTTWNIDGCSEGQIVRLFEEMFAAAIVYTQK 60
            MKNHY +PSPPDLGWDDL  + RT+D +SL TWNIDG SE Q++  F+EM  AA  Y+ K
Sbjct: 617  MKNHYPQPSPPDLGWDDLHHEKRTYDGQSLITWNIDGYSEAQMMNTFQEMLLAATAYSTK 676

Query: 61   FSQKDTTQILVSGFTGILRSWWHNHLTAENRELILNHQIEEIAKGVDGIEYTTMAPNAVN 120
             S  +T QIL+ GF G LRSWWHN LT ++R+ IL      +             P+ VN
Sbjct: 677  KSTYETAQILILGFNGNLRSWWHNLLTEQDRQRILTATRTVVKTENTSTPIQVEEPDMVN 736

Query: 121  ALIYTTLKNFVGHTTLYSDQATAALLSLKCPKISDFKWYKDTFLMCLYILTTCHDVLWKH 180
             L+YT  K+F+G T ++ + AT ALL LKC K+S +KWYKDTF+  LY LTTC   +WK 
Sbjct: 737  QLLYTMTKHFIGSTQIHLNLATEALLGLKCHKMSRYKWYKDTFMARLYTLTTCGADIWKQ 796

Query: 181  KYVEGLP----------------------------------------------------- 240
            K+VEGLP                                                     
Sbjct: 797  KFVEGLPHYISQKFYQTMTANSVNQQIDWANLTYGDISSTVQMICVNLCTENKHTTKVIK 856

Query: 241  ------------SQYGLEDLP----PSKRKNGSFKKTFKKQKNIISEAPRRRQRF----K 300
                         QYGL   P      K+K  S KK F+K K    E+P+RR+R     K
Sbjct: 857  DSDYRKELGTFCKQYGLSQGPKEEKKKKKKRYSSKKFFRKSKTKDQESPQRRKRHYNKGK 916

Query: 301  SKKKQSTK-ETVCFKCKKPGHYANRCPLVKKVNKLEIDEDTKQSLL-RILNDYSSETDSS 360
            SKK  S+K  T+CFKC + GHYANRCPL  K+N + IDE+TKQSLL  I +D  + + + 
Sbjct: 917  SKKGYSSKTHTICFKCNQKGHYANRCPLKDKINAMTIDEETKQSLLYAIRSDDDTTSQTE 976

Query: 361  SSSDEDLGRINEILEE-SSGESSF--SSEKSEDDGAIP--------CSGCINVLTSYQKD 420
            SSS+ED   IN + EE SS E  F   S+ S+D+GAIP        CSG INV+T  Q+ 
Sbjct: 977  SSSEEDY--INILQEEGSSSEEEFYSQSDSSDDEGAIPCTGRCAGKCSGHINVITKDQET 1036

Query: 421  LLDLIDEIPDKEAQKSILLKLRSSLKAQEVRP--RNPVDFSFQSILNQLQSEGTTSVKIP 480
            L DLI++IPD+EA+++ LLKL+ SL+ Q  +   +NP+ +S+Q ILN+++ E    +++ 
Sbjct: 1037 LFDLIEQIPDEEAKRTCLLKLKQSLEEQAPQKAIQNPIMYSYQDILNRVKGEAKMPIQVE 1096

Query: 481  DMQQEIKSLKKEVAENKTRITNLEEAFYLIQEKTIFQDIQKGPSNESGPSSQNEDG---- 540
            D+  E+K+LK+EVAENK R+  LE AF        FQ  Q      +    +   G    
Sbjct: 1097 DLHHEVKTLKREVAENKQRLIYLETAF------QAFQGSQASKEESTSDFERKSAGKALL 1156

Query: 541  ------INAISKVFNKKWLSNITLKIQDSKLETIALIDSGADQNVIREGLIPTRYCEMTK 564
                  IN+IS++ N+KW+S I  K++D +LE +ALIDSGADQNVI+EGL+P+RY E TK
Sbjct: 1157 IEEIGTINSISRIQNQKWMSKIVFKVKDFQLEALALIDSGADQNVIQEGLVPSRYFEKTK 1216

BLAST of Lag0005960 vs. NCBI nr
Match: KAA0052109.1 (Enzymatic polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 489.6 bits (1259), Expect = 3.7e-134
Identity = 293/660 (44.39%), Postives = 390/660 (59.09%), Query Frame = 0

Query: 1    MKNHYRRPSPPDLGWDDLAPDHRTFDPKSLTTWNIDGCSEGQIVRLFEEMFAAAIVYTQK 60
            MKNHY +PSPPDLGWDDL  + RT+D +SL TWNIDG SE Q++  F+EM  AA  Y+ K
Sbjct: 616  MKNHYPQPSPPDLGWDDLHHEKRTYDGQSLITWNIDGYSEAQMMNTFQEMLLAATAYSTK 675

Query: 61   FSQKDTTQILVSGFTGILRSWWHNHLTAENRELILNHQIEEIAKGVDGIEYTTMAPNAVN 120
             S  +T QIL+ GF G LRSWWHN LT ++R+ IL      +             P+ VN
Sbjct: 676  KSTYETAQILILGFNGNLRSWWHNLLTEQDRQRILTATRTVVKTENTSTPIQVEEPDMVN 735

Query: 121  ALIYTTLKNFVGHTTLYSDQATAALLSLKCPKISDFKWYKDTFLMCLYILTTCHDVLWKH 180
             L+YT  K+F+G T ++ + AT ALL LK  K+S +KWYKDTF+  LY LTTC   +WK 
Sbjct: 736  QLLYTMTKHFIGSTQIHLNLATEALLGLKYHKMSRYKWYKDTFMARLYTLTTCGADIWKQ 795

Query: 181  KYVEGLP----------------------------------------------------- 240
            K+VEGLP                                                     
Sbjct: 796  KFVEGLPHYISQKFYQTMTANSVNQQIDWANLTYGDISSTVQMINVNLCTENKHTTKVIK 855

Query: 241  ------------SQYGLEDLP----PSKRKNGSFKKTFKKQKNIISEAPRRRQ----RFK 300
                         QYGL   P      K+K  S KK F+K K    E+P+RR+    + K
Sbjct: 856  DSDYRKELGTFCKQYGLSQGPKEEKKKKKKRYSSKKFFRKGKVKDQESPQRRRHHYYKGK 915

Query: 301  SKKKQSTK-ETVCFKCKKPGHYANRCPLVKKVNKLEIDEDTKQSLL---RILNDYSSETD 360
             KKK S+K  T+CFKC + GHYANRCPL  K+N L IDE+TKQSLL   R+ +D SS+T+
Sbjct: 916  GKKKYSSKTNTICFKCNQKGHYANRCPLKDKINALTIDEETKQSLLYAIRMDDDTSSQTE 975

Query: 361  SSSSSDEDLGRINEILEE-SSGESSF--SSEKSEDDGAIP--------CSGCINVLTSYQ 420
            SSS  D     IN + EE SS E  F   S+ S+D+GAIP        CSG INV+T  Q
Sbjct: 976  SSSEED----YINILQEEGSSSEEEFYSQSDSSDDEGAIPCTGRCAGKCSGHINVITKDQ 1035

Query: 421  KDLLDLIDEIPDKEAQKSILLKLRSSLKAQEVRP--RNPVDFSFQSILNQLQSEGTTSVK 480
            + L  LI++IPD+EA+++ LLKL+ SL+ Q  +   +NP+ +S+Q ILN+++ E    ++
Sbjct: 1036 ETLFYLIEQIPDEEAKRTCLLKLKQSLEEQAPQKAIQNPIMYSYQDILNRVKGEAKMPIQ 1095

Query: 481  IPDMQQEIKSLKKEVAENKTRITNLEEAFYLIQEKTIFQDIQKGPSNE-------SGPSS 540
            + D+  E+K+LK+EVAENK R+  LE AF   QE  + ++  +   N+            
Sbjct: 1096 VEDLHHEVKTLKREVAENKQRLIYLENAFQAFQESQVLKENSETSRNDFERKIARKALLI 1155

Query: 541  QNEDGINAISKVFNKKWLSNITLKIQDSKLETIALIDSGADQNVIREGLIPTRYCEMTKQ 564
             +   IN+ISKV NKKW+S I  K++D +LET+ALIDSGADQNVI+EGL+P++Y E TK+
Sbjct: 1156 DDSGKINSISKVHNKKWMSKIVFKVKDFQLETLALIDSGADQNVIQEGLVPSKYFEKTKE 1215

BLAST of Lag0005960 vs. NCBI nr
Match: KAA0057417.1 (Enzymatic polyprotein [Cucumis melo var. makuwa] >TYK30116.1 Enzymatic polyprotein [Cucumis melo var. makuwa])

HSP 1 Score: 428.7 bits (1101), Expect = 7.7e-116
Identity = 265/621 (42.67%), Postives = 356/621 (57.33%), Query Frame = 0

Query: 1    MKNHYRRPSPPDLGWDDLAPDHRTFDPKSLTTWNIDGCSEGQIVRLFEEMFAAAIVYTQK 60
            MKNHY +PSPPDLGWDDL  + RT+D +SL TWN DG  E Q++  F+EM  AA  Y+ K
Sbjct: 508  MKNHYPQPSPPDLGWDDLHHEKRTYDGQSLITWNTDGYFEAQMMNTFQEMLLAATAYSTK 567

Query: 61   FSQKDTTQILVSGFTGILRSWWHNHLTAENRELILNHQIEEIAKGVDGIEYTTMAPNAVN 120
             S  +T QIL+ GF G LRSWWHN LT ++R+ IL      +             P+ VN
Sbjct: 568  KSTYETAQILILGFNGNLRSWWHNLLTEQDRQRILTATRTVVKTENSSTPIQVEEPDMVN 627

Query: 121  ALIYTTLKNFVGHTTLYSDQATAALLSLKCPKISDFKWYKDTFLMCLYILTTCHDVLWKH 180
             L+YT  K+F+G T ++ + AT ALL LKC K+S +KWYKDTF+  LY LTTC   +WK 
Sbjct: 628  QLLYTMTKHFIGSTQIHLNLATEALLGLKCHKMSRYKWYKDTFMARLYTLTTCGADIWKQ 687

Query: 181  KYVEGLP----------------------------------------------------- 240
            K+VEGLP                                                     
Sbjct: 688  KFVEGLPHYISQKFYQTMTENSVNQQIDWANLTYGDISSTVQMICVNLCTENKHTTKVIK 747

Query: 241  ------------SQYGLEDLP---PSKRKNGSFKKTFKKQKNIISEAPRRRQ----RFKS 300
                         QYGL   P     K+K  S KK F++ K    E+PRRR+    + K 
Sbjct: 748  DSDYRKELGTFCKQYGLSQGPKEEKKKKKKYSSKKFFRRSKPKDQESPRRRKHHYNKGKG 807

Query: 301  KKKQSTK-ETVCFKCKKPGHYANRCPLVKKVNKLEIDEDTKQSLL---RILNDYSSETDS 360
            KK+ S+K  T+CFKC + GHYANRCPL  K+N L IDE TKQS+L   R  +D SS+T+S
Sbjct: 808  KKRYSSKTNTICFKCNQKGHYANRCPLQDKINALTIDEKTKQSILYAIRSDDDTSSQTES 867

Query: 361  SSSSDEDLGRINEILEE-SSGESSF--SSEKSEDDGAIPCSG-----C---INVLTSYQK 420
            SS  D     IN + EE SS E  F   S+ S+D+GAIPC+G     C   INV+T  Q+
Sbjct: 868  SSEED----YINILQEEGSSSEEEFYSQSDSSDDEGAIPCTGRCAGKCFGHINVITKDQE 927

Query: 421  DLLDLIDEIPDKEAQKSILLKLRSSLKAQ--EVRPRNPVDFSFQSILNQLQSEGTTSVKI 480
             L DLI++I D+EA+++ LLKL+ SL+ Q  +   +N + + +Q I N+++ E    +++
Sbjct: 928  TLFDLIEQILDEEAKRTYLLKLKQSLEEQVPQKTIQNLIMYWYQDIPNRVKGEAKIPIQV 987

Query: 481  PDMQQEIKSLKKEVAENKTRITNLEEAFYLIQEKTIFQDIQKGPSNE-----SGPSSQNE 526
             D+  E+K LK+EV ENK R+  LE AF   QE  + ++  +  +N+     +G +   E
Sbjct: 988  EDLHHEVKILKREVTENKQRLIYLENAFQAFQESQVLKENSETSTNDFKRKIAGKALLIE 1047

BLAST of Lag0005960 vs. ExPASy TrEMBL
Match: A0A5A7UR29 (Enzymatic polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold486G00660 PE=4 SV=1)

HSP 1 Score: 491.5 bits (1264), Expect = 4.7e-135
Identity = 294/661 (44.48%), Postives = 388/661 (58.70%), Query Frame = 0

Query: 1    MKNHYRRPSPPDLGWDDLAPDHRTFDPKSLTTWNIDGCSEGQIVRLFEEMFAAAIVYTQK 60
            MKNHY +PSPPDLGWDDL  + RT+D +SL TWNIDG SE Q++  F+EM  AA  Y+ K
Sbjct: 617  MKNHYPQPSPPDLGWDDLHHEKRTYDGQSLITWNIDGYSEAQMMNTFQEMLLAATAYSTK 676

Query: 61   FSQKDTTQILVSGFTGILRSWWHNHLTAENRELILNHQIEEIAKGVDGIEYTTMAPNAVN 120
             S  +T QIL+ GF G LRSWWHN LT ++R+ IL      +             P+ VN
Sbjct: 677  KSTYETAQILILGFNGNLRSWWHNLLTEQDRQRILTATRTVVKTENTSTPIQVEEPDMVN 736

Query: 121  ALIYTTLKNFVGHTTLYSDQATAALLSLKCPKISDFKWYKDTFLMCLYILTTCHDVLWKH 180
             L+YT  K+F+G T ++ + AT ALL LKC K+S +KWYKDTF+  LY LTTC   +WK 
Sbjct: 737  QLLYTMTKHFIGSTQIHLNLATEALLGLKCHKMSRYKWYKDTFMARLYTLTTCGADIWKQ 796

Query: 181  KYVEGLP----------------------------------------------------- 240
            K+VEGLP                                                     
Sbjct: 797  KFVEGLPHYISQKFYQTMTANSVNQQIDWANLTYGDISSTVQMICVNLCTENKHTTKVIK 856

Query: 241  ------------SQYGLEDLP----PSKRKNGSFKKTFKKQKNIISEAPRRRQRF----K 300
                         QYGL   P      K+K  S KK F+K K    E+PRRR+R     K
Sbjct: 857  DSDYRKELGTFCKQYGLSQGPKEEKKKKKKRYSSKKFFRKSKAKDQESPRRRRRHYNKGK 916

Query: 301  SKKKQSTK-ETVCFKCKKPGHYANRCPLVKKVNKLEIDEDTKQSLL-RILNDYSSETDSS 360
            SKK  S+K  T+CFKC + GHYANRCPL  K+N + IDE+TKQSLL  I +D  + + + 
Sbjct: 917  SKKGYSSKTHTICFKCNQKGHYANRCPLKDKINAMTIDEETKQSLLYAIRSDDDTTSQTE 976

Query: 361  SSSDEDLGRINEILEE-SSGESSF--SSEKSEDDGAIP--------CSGCINVLTSYQKD 420
            SSS+ED   IN + EE SS E  F   S+ S+D+GAIP        CSG INV+T  Q+ 
Sbjct: 977  SSSEEDY--INILQEEGSSSEEEFYSQSDSSDDEGAIPCTGRCAGKCSGHINVITKDQET 1036

Query: 421  LLDLIDEIPDKEAQKSILLKLRSSLKAQEVRP--RNPVDFSFQSILNQLQSEGTTSVKIP 480
            L DLI++IPD+EA+++ LLKL+ SL+ Q  +   +NP+ +S+Q ILN+++ E    +++ 
Sbjct: 1037 LFDLIEQIPDEEAKRTCLLKLKQSLEEQAPQKAIQNPIMYSYQDILNRVKGEAKMPIQVE 1096

Query: 481  DMQQEIKSLKKEVAENKTRITNLEEAFYLIQEKTIFQDIQKGPSNESGPSSQNEDG---- 540
            D+  E+K+LK+EVAENK R+  LE AF        FQ  Q      +    +   G    
Sbjct: 1097 DLHHEVKTLKREVAENKQRLIYLETAF------QAFQGSQASKEESTSDFERKSAGKALL 1156

Query: 541  ------INAISKVFNKKWLSNITLKIQDSKLETIALIDSGADQNVIREGLIPTRYCEMTK 564
                  IN+ISK+ N+KW+S I  K++D +LE +ALIDSGADQNVI+EGL+P+RY E TK
Sbjct: 1157 IEEIGTINSISKIQNQKWMSKIVFKVKDFQLEALALIDSGADQNVIQEGLVPSRYFEKTK 1216

BLAST of Lag0005960 vs. ExPASy TrEMBL
Match: A0A5D3BG41 (Enzymatic polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold565G00200 PE=4 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 1.8e-134
Identity = 293/660 (44.39%), Postives = 390/660 (59.09%), Query Frame = 0

Query: 1    MKNHYRRPSPPDLGWDDLAPDHRTFDPKSLTTWNIDGCSEGQIVRLFEEMFAAAIVYTQK 60
            MKNHY +PSPPDLGWDDL  + RT+D +SL TWNIDG SE Q++  F+EM  AA  Y+ K
Sbjct: 616  MKNHYPQPSPPDLGWDDLHHEKRTYDGQSLITWNIDGYSEAQMMNTFQEMLLAATAYSTK 675

Query: 61   FSQKDTTQILVSGFTGILRSWWHNHLTAENRELILNHQIEEIAKGVDGIEYTTMAPNAVN 120
             S  +T QIL+ GF G LRSWWHN LT ++R+ IL      +             P+ VN
Sbjct: 676  KSTYETAQILILGFNGNLRSWWHNLLTEQDRQRILTATRTVVKTENTSTPIQVEEPDMVN 735

Query: 121  ALIYTTLKNFVGHTTLYSDQATAALLSLKCPKISDFKWYKDTFLMCLYILTTCHDVLWKH 180
             L+YT  K+F+G T ++ + AT ALL LK  K+S +KWYKDTF+  LY LTTC   +WK 
Sbjct: 736  QLLYTMTKHFIGSTQIHLNLATEALLGLKYHKMSRYKWYKDTFMARLYTLTTCGADIWKQ 795

Query: 181  KYVEGLP----------------------------------------------------- 240
            K+VEGLP                                                     
Sbjct: 796  KFVEGLPHYISQKFYQTMTANSVNQQIDWANLTYGDISSTVQMINVNLCTENKHTTKVIK 855

Query: 241  ------------SQYGLEDLP----PSKRKNGSFKKTFKKQKNIISEAPRRRQ----RFK 300
                         QYGL   P      K+K  S KK F+K K    E+P+RR+    + K
Sbjct: 856  DSDYRKELGTFCKQYGLSQGPKEEKKKKKKRYSSKKFFRKGKVKDQESPQRRRHHYYKGK 915

Query: 301  SKKKQSTK-ETVCFKCKKPGHYANRCPLVKKVNKLEIDEDTKQSLL---RILNDYSSETD 360
             KKK S+K  T+CFKC + GHYANRCPL  K+N L IDE+TKQSLL   R+ +D SS+T+
Sbjct: 916  GKKKYSSKTNTICFKCNQKGHYANRCPLKDKINALTIDEETKQSLLYAIRMDDDTSSQTE 975

Query: 361  SSSSSDEDLGRINEILEE-SSGESSF--SSEKSEDDGAIP--------CSGCINVLTSYQ 420
            SSS  D     IN + EE SS E  F   S+ S+D+GAIP        CSG INV+T  Q
Sbjct: 976  SSSEED----YINILQEEGSSSEEEFYSQSDSSDDEGAIPCTGRCAGKCSGHINVITKDQ 1035

Query: 421  KDLLDLIDEIPDKEAQKSILLKLRSSLKAQEVRP--RNPVDFSFQSILNQLQSEGTTSVK 480
            + L  LI++IPD+EA+++ LLKL+ SL+ Q  +   +NP+ +S+Q ILN+++ E    ++
Sbjct: 1036 ETLFYLIEQIPDEEAKRTCLLKLKQSLEEQAPQKAIQNPIMYSYQDILNRVKGEAKMPIQ 1095

Query: 481  IPDMQQEIKSLKKEVAENKTRITNLEEAFYLIQEKTIFQDIQKGPSNE-------SGPSS 540
            + D+  E+K+LK+EVAENK R+  LE AF   QE  + ++  +   N+            
Sbjct: 1096 VEDLHHEVKTLKREVAENKQRLIYLENAFQAFQESQVLKENSETSRNDFERKIARKALLI 1155

Query: 541  QNEDGINAISKVFNKKWLSNITLKIQDSKLETIALIDSGADQNVIREGLIPTRYCEMTKQ 564
             +   IN+ISKV NKKW+S I  K++D +LET+ALIDSGADQNVI+EGL+P++Y E TK+
Sbjct: 1156 DDSGKINSISKVHNKKWMSKIVFKVKDFQLETLALIDSGADQNVIQEGLVPSKYFEKTKE 1215

BLAST of Lag0005960 vs. ExPASy TrEMBL
Match: A0A5D3BEY3 (Enzymatic polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold690G00300 PE=4 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 1.8e-134
Identity = 292/661 (44.18%), Postives = 388/661 (58.70%), Query Frame = 0

Query: 1    MKNHYRRPSPPDLGWDDLAPDHRTFDPKSLTTWNIDGCSEGQIVRLFEEMFAAAIVYTQK 60
            MKNHY +PSPPDLGWDDL  + RT+D +SL TWNIDG SE Q++  F+EM  AA  Y+ K
Sbjct: 617  MKNHYPQPSPPDLGWDDLHHEKRTYDGQSLITWNIDGYSEAQMMNTFQEMLLAATAYSTK 676

Query: 61   FSQKDTTQILVSGFTGILRSWWHNHLTAENRELILNHQIEEIAKGVDGIEYTTMAPNAVN 120
             S  +T QIL+ GF G LRSWWHN LT ++R+ IL      +             P+ VN
Sbjct: 677  KSTYETAQILILGFNGNLRSWWHNLLTEQDRQRILTATRTVVKTENTSTPIQVEEPDMVN 736

Query: 121  ALIYTTLKNFVGHTTLYSDQATAALLSLKCPKISDFKWYKDTFLMCLYILTTCHDVLWKH 180
             L+YT  K+F+G T ++ + AT ALL LKC K+S +KWYKDTF+  LY LTTC   +WK 
Sbjct: 737  QLLYTMTKHFIGSTQIHLNLATEALLGLKCHKMSRYKWYKDTFMARLYTLTTCGADIWKQ 796

Query: 181  KYVEGLP----------------------------------------------------- 240
            K+VEGLP                                                     
Sbjct: 797  KFVEGLPHYISQKFYQTMTANSVNQQIDWANLTYGDISSTVQMICVNLCTENKHTTKVIK 856

Query: 241  ------------SQYGLEDLP----PSKRKNGSFKKTFKKQKNIISEAPRRRQRF----K 300
                         QYGL   P      K+K  S KK F+K K    E+P+RR+R     K
Sbjct: 857  DSDYRKELGTFCKQYGLSQGPKEEKKKKKKRYSSKKFFRKSKTKDQESPQRRKRHYNKGK 916

Query: 301  SKKKQSTK-ETVCFKCKKPGHYANRCPLVKKVNKLEIDEDTKQSLL-RILNDYSSETDSS 360
            SKK  S+K  T+CFKC + GHYANRCPL  K+N + IDE+TKQSLL  I +D  + + + 
Sbjct: 917  SKKGYSSKTHTICFKCNQKGHYANRCPLKDKINAMTIDEETKQSLLYAIRSDDDTTSQTE 976

Query: 361  SSSDEDLGRINEILEE-SSGESSF--SSEKSEDDGAIP--------CSGCINVLTSYQKD 420
            SSS+ED   IN + EE SS E  F   S+ S+D+GAIP        CSG INV+T  Q+ 
Sbjct: 977  SSSEEDY--INILQEEGSSSEEEFYSQSDSSDDEGAIPCTGRCAGKCSGHINVITKDQET 1036

Query: 421  LLDLIDEIPDKEAQKSILLKLRSSLKAQEVRP--RNPVDFSFQSILNQLQSEGTTSVKIP 480
            L DLI++IPD+EA+++ LLKL+ SL+ Q  +   +NP+ +S+Q ILN+++ E    +++ 
Sbjct: 1037 LFDLIEQIPDEEAKRTCLLKLKQSLEEQAPQKAIQNPIMYSYQDILNRVKGEAKMPIQVE 1096

Query: 481  DMQQEIKSLKKEVAENKTRITNLEEAFYLIQEKTIFQDIQKGPSNESGPSSQNEDG---- 540
            D+  E+K+LK+EVAENK R+  LE AF        FQ  Q      +    +   G    
Sbjct: 1097 DLHHEVKTLKREVAENKQRLIYLETAF------QAFQGSQASKEESTSDFERKSAGKALL 1156

Query: 541  ------INAISKVFNKKWLSNITLKIQDSKLETIALIDSGADQNVIREGLIPTRYCEMTK 564
                  IN+IS++ N+KW+S I  K++D +LE +ALIDSGADQNVI+EGL+P+RY E TK
Sbjct: 1157 IEEIGTINSISRIQNQKWMSKIVFKVKDFQLEALALIDSGADQNVIQEGLVPSRYFEKTK 1216

BLAST of Lag0005960 vs. ExPASy TrEMBL
Match: A0A5A7UF59 (Enzymatic polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold578G00970 PE=4 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 1.8e-134
Identity = 293/660 (44.39%), Postives = 390/660 (59.09%), Query Frame = 0

Query: 1    MKNHYRRPSPPDLGWDDLAPDHRTFDPKSLTTWNIDGCSEGQIVRLFEEMFAAAIVYTQK 60
            MKNHY +PSPPDLGWDDL  + RT+D +SL TWNIDG SE Q++  F+EM  AA  Y+ K
Sbjct: 616  MKNHYPQPSPPDLGWDDLHHEKRTYDGQSLITWNIDGYSEAQMMNTFQEMLLAATAYSTK 675

Query: 61   FSQKDTTQILVSGFTGILRSWWHNHLTAENRELILNHQIEEIAKGVDGIEYTTMAPNAVN 120
             S  +T QIL+ GF G LRSWWHN LT ++R+ IL      +             P+ VN
Sbjct: 676  KSTYETAQILILGFNGNLRSWWHNLLTEQDRQRILTATRTVVKTENTSTPIQVEEPDMVN 735

Query: 121  ALIYTTLKNFVGHTTLYSDQATAALLSLKCPKISDFKWYKDTFLMCLYILTTCHDVLWKH 180
             L+YT  K+F+G T ++ + AT ALL LK  K+S +KWYKDTF+  LY LTTC   +WK 
Sbjct: 736  QLLYTMTKHFIGSTQIHLNLATEALLGLKYHKMSRYKWYKDTFMARLYTLTTCGADIWKQ 795

Query: 181  KYVEGLP----------------------------------------------------- 240
            K+VEGLP                                                     
Sbjct: 796  KFVEGLPHYISQKFYQTMTANSVNQQIDWANLTYGDISSTVQMINVNLCTENKHTTKVIK 855

Query: 241  ------------SQYGLEDLP----PSKRKNGSFKKTFKKQKNIISEAPRRRQ----RFK 300
                         QYGL   P      K+K  S KK F+K K    E+P+RR+    + K
Sbjct: 856  DSDYRKELGTFCKQYGLSQGPKEEKKKKKKRYSSKKFFRKGKVKDQESPQRRRHHYYKGK 915

Query: 301  SKKKQSTK-ETVCFKCKKPGHYANRCPLVKKVNKLEIDEDTKQSLL---RILNDYSSETD 360
             KKK S+K  T+CFKC + GHYANRCPL  K+N L IDE+TKQSLL   R+ +D SS+T+
Sbjct: 916  GKKKYSSKTNTICFKCNQKGHYANRCPLKDKINALTIDEETKQSLLYAIRMDDDTSSQTE 975

Query: 361  SSSSSDEDLGRINEILEE-SSGESSF--SSEKSEDDGAIP--------CSGCINVLTSYQ 420
            SSS  D     IN + EE SS E  F   S+ S+D+GAIP        CSG INV+T  Q
Sbjct: 976  SSSEED----YINILQEEGSSSEEEFYSQSDSSDDEGAIPCTGRCAGKCSGHINVITKDQ 1035

Query: 421  KDLLDLIDEIPDKEAQKSILLKLRSSLKAQEVRP--RNPVDFSFQSILNQLQSEGTTSVK 480
            + L  LI++IPD+EA+++ LLKL+ SL+ Q  +   +NP+ +S+Q ILN+++ E    ++
Sbjct: 1036 ETLFYLIEQIPDEEAKRTCLLKLKQSLEEQAPQKAIQNPIMYSYQDILNRVKGEAKMPIQ 1095

Query: 481  IPDMQQEIKSLKKEVAENKTRITNLEEAFYLIQEKTIFQDIQKGPSNE-------SGPSS 540
            + D+  E+K+LK+EVAENK R+  LE AF   QE  + ++  +   N+            
Sbjct: 1096 VEDLHHEVKTLKREVAENKQRLIYLENAFQAFQESQVLKENSETSRNDFERKIARKALLI 1155

Query: 541  QNEDGINAISKVFNKKWLSNITLKIQDSKLETIALIDSGADQNVIREGLIPTRYCEMTKQ 564
             +   IN+ISKV NKKW+S I  K++D +LET+ALIDSGADQNVI+EGL+P++Y E TK+
Sbjct: 1156 DDSGKINSISKVHNKKWMSKIVFKVKDFQLETLALIDSGADQNVIQEGLVPSKYFEKTKE 1215

BLAST of Lag0005960 vs. ExPASy TrEMBL
Match: A0A5A7URX9 (Enzymatic polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold216G00980 PE=4 SV=1)

HSP 1 Score: 428.7 bits (1101), Expect = 3.7e-116
Identity = 265/621 (42.67%), Postives = 356/621 (57.33%), Query Frame = 0

Query: 1    MKNHYRRPSPPDLGWDDLAPDHRTFDPKSLTTWNIDGCSEGQIVRLFEEMFAAAIVYTQK 60
            MKNHY +PSPPDLGWDDL  + RT+D +SL TWN DG  E Q++  F+EM  AA  Y+ K
Sbjct: 508  MKNHYPQPSPPDLGWDDLHHEKRTYDGQSLITWNTDGYFEAQMMNTFQEMLLAATAYSTK 567

Query: 61   FSQKDTTQILVSGFTGILRSWWHNHLTAENRELILNHQIEEIAKGVDGIEYTTMAPNAVN 120
             S  +T QIL+ GF G LRSWWHN LT ++R+ IL      +             P+ VN
Sbjct: 568  KSTYETAQILILGFNGNLRSWWHNLLTEQDRQRILTATRTVVKTENSSTPIQVEEPDMVN 627

Query: 121  ALIYTTLKNFVGHTTLYSDQATAALLSLKCPKISDFKWYKDTFLMCLYILTTCHDVLWKH 180
             L+YT  K+F+G T ++ + AT ALL LKC K+S +KWYKDTF+  LY LTTC   +WK 
Sbjct: 628  QLLYTMTKHFIGSTQIHLNLATEALLGLKCHKMSRYKWYKDTFMARLYTLTTCGADIWKQ 687

Query: 181  KYVEGLP----------------------------------------------------- 240
            K+VEGLP                                                     
Sbjct: 688  KFVEGLPHYISQKFYQTMTENSVNQQIDWANLTYGDISSTVQMICVNLCTENKHTTKVIK 747

Query: 241  ------------SQYGLEDLP---PSKRKNGSFKKTFKKQKNIISEAPRRRQ----RFKS 300
                         QYGL   P     K+K  S KK F++ K    E+PRRR+    + K 
Sbjct: 748  DSDYRKELGTFCKQYGLSQGPKEEKKKKKKYSSKKFFRRSKPKDQESPRRRKHHYNKGKG 807

Query: 301  KKKQSTK-ETVCFKCKKPGHYANRCPLVKKVNKLEIDEDTKQSLL---RILNDYSSETDS 360
            KK+ S+K  T+CFKC + GHYANRCPL  K+N L IDE TKQS+L   R  +D SS+T+S
Sbjct: 808  KKRYSSKTNTICFKCNQKGHYANRCPLQDKINALTIDEKTKQSILYAIRSDDDTSSQTES 867

Query: 361  SSSSDEDLGRINEILEE-SSGESSF--SSEKSEDDGAIPCSG-----C---INVLTSYQK 420
            SS  D     IN + EE SS E  F   S+ S+D+GAIPC+G     C   INV+T  Q+
Sbjct: 868  SSEED----YINILQEEGSSSEEEFYSQSDSSDDEGAIPCTGRCAGKCFGHINVITKDQE 927

Query: 421  DLLDLIDEIPDKEAQKSILLKLRSSLKAQ--EVRPRNPVDFSFQSILNQLQSEGTTSVKI 480
             L DLI++I D+EA+++ LLKL+ SL+ Q  +   +N + + +Q I N+++ E    +++
Sbjct: 928  TLFDLIEQILDEEAKRTYLLKLKQSLEEQVPQKTIQNLIMYWYQDIPNRVKGEAKIPIQV 987

Query: 481  PDMQQEIKSLKKEVAENKTRITNLEEAFYLIQEKTIFQDIQKGPSNE-----SGPSSQNE 526
             D+  E+K LK+EV ENK R+  LE AF   QE  + ++  +  +N+     +G +   E
Sbjct: 988  EDLHHEVKILKREVTENKQRLIYLENAFQAFQESQVLKENSETSTNDFKRKIAGKALLIE 1047

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAA0056776.19.7e-13544.48Enzymatic polyprotein [Cucumis melo var. makuwa][more]
TYJ98087.13.7e-13444.39Enzymatic polyprotein [Cucumis melo var. makuwa][more]
TYJ97599.13.7e-13444.18Enzymatic polyprotein [Cucumis melo var. makuwa][more]
KAA0052109.13.7e-13444.39Enzymatic polyprotein [Cucumis melo var. makuwa][more]
KAA0057417.17.7e-11642.67Enzymatic polyprotein [Cucumis melo var. makuwa] >TYK30116.1 Enzymatic polyprote... [more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A5A7UR294.7e-13544.48Enzymatic polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold48... [more]
A0A5D3BG411.8e-13444.39Enzymatic polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold56... [more]
A0A5D3BEY31.8e-13444.18Enzymatic polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold69... [more]
A0A5A7UF591.8e-13444.39Enzymatic polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold57... [more]
A0A5A7URX93.7e-11642.67Enzymatic polyprotein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold21... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 391..418
NoneNo IPR availableGENE3D4.10.60.10coord: 230..288
e-value: 1.9E-6
score: 30.0
NoneNo IPR availablePANTHERPTHR33054FAMILY NOT NAMEDcoord: 25..188
coord: 197..558
NoneNo IPR availableCDDcd00303retropepsin_likecoord: 466..553
e-value: 6.42743E-12
score: 60.0428
IPR001878Zinc finger, CCHC-typeSMARTSM00343c2hcfinal6coord: 238..254
e-value: 0.0011
score: 28.3
IPR001878Zinc finger, CCHC-typePFAMPF00098zf-CCHCcoord: 238..253
e-value: 6.3E-5
score: 22.9
IPR001878Zinc finger, CCHC-typePROSITEPS50158ZF_CCHCcoord: 239..253
score: 9.883478
IPR018061RetropepsinsPFAMPF00077RVPcoord: 465..555
e-value: 1.7E-8
score: 34.7
IPR021109Aspartic peptidase domain superfamilyGENE3D2.40.70.10Acid Proteasescoord: 462..560
e-value: 1.2E-6
score: 30.3
IPR021109Aspartic peptidase domain superfamilySUPERFAMILY50630Acid proteasescoord: 470..557
IPR001995Peptidase A2A, retrovirus, catalyticPROSITEPS50175ASP_PROT_RETROVcoord: 475..551
score: 10.927424
IPR036875Zinc finger, CCHC-type superfamilySUPERFAMILY57756Retrovirus zinc finger-like domainscoord: 221..258

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Lag0005960.1Lag0005960.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006508 proteolysis
molecular_function GO:0004190 aspartic-type endopeptidase activity
molecular_function GO:0003676 nucleic acid binding
molecular_function GO:0008270 zinc ion binding