Lag0005854 (gene) Sponge gourd (AG‐4) v1

Overview


Name	Lag0005854
Type	gene
Organism	Luffa acutangula (Sponge gourd (AG‐4) v1)
Description	Photosystem I reaction center subunit IV
Location	chr6: 32127689 .. 32129065 (+)
RNA-Seq Expression	Lag0005854
Synteny	Lag0005854

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTGCCTCTAACCTTTCATGCATTGCTTCTGGGGTGGTCATTCCTTCTCCAACCACCATTTCATCCTCTTCTAGGTTATCTTCTGTCGCCTTTTTCCCCAAGAATTCCCACTCCCTTTCAAGATCATCCAGATTGGTTATCCGGGCCGCCGCCGAAGACGCTGCTCCCCCACCCCCTGCTGCCGCCGCCACCGCCGATGCCCCAGCCGAAGAGCCTAAGCCTAAGCCTGCCCCAATTGGCCCTAAGAGAGGTGCCAAGGTGTGTGACAATTTTCTTTTTGTTTTGGGTTTTTTAGTTTGTTTAGATCTGAATTTGAACATCTGATCTCTAAGTCGTTTCCATAAGGTGAGTAATAATTTTTAATCATAGTCGTCGTAGAAATGATGCCGATGATGATGATTTCTTTTCAATAATTTTAGGCCCTTATATTAAATTTTGTTGTTGTTATTGATGTTTGATTCATTTTAATATCTTGTCATGAACAGGTCAAGATTCTAAGGAGAGAGTCCTACTGGTACAAGGATGTTGGATCTGTTGTAACAGTTGACCAGGTTGGACACTTTAAATTTGATTCATTTATCATTTTTGTTTTTGTTTTTGTGTGTGTGCAAACATAATTTTTTTTTGTTATATTTTTCTTTCTTGCATTTTATTTACATTTTTGAGTGAGATTTACACTCACAAAATTAAGTAGATAAGATTTATGAGGAACCATGTCCTGTTAAGGGGTCAAGGCTGCTCTGTTTTGGGATTGGTTTTAGCAGATGTTAAAAATGGTTATTAGAGAATTTTAATAGTTTAGGTGTTGGTAAAATTGACTATATATATATTAATTTAAATAAATTAGTTTTTCTATTACGATGATCCGTAAAATCTAGTAATCAATTATAAGCTTTTGAGATTGATTTTTCAAAGAAACTACTGTTAGATTATATGGGCTTCCATCTCAAAACAAATTGGCAATGAGTGGAATGACCCTCGTAGCTTATATATTGTGAGGTCTCCACCAATCTTTCCGATGTGAGATCCTCAACAACTACAGCAAACTTTAAAAATTACTAGTTTAAAATTGAATTTTACTAAACACTAATTTGATTTATACTACGTCAGCAAATTGAGACTAAGATGTAATCTTGACCGGTGTTTAAGTATAATTTAAACACCAAGTGAGGTCTAGAATGTTTCTATTGGGACAAATTTAACAGTACTCACTAATTGTTTTGTTAAGATTTTAAATAATATGCTTAATGTGTAAATGATATTTTGCAGGACCCAAACACTCGATATCCAGTTGTGGTTAGATTTAACAAAGTAAATTATGCAAGTGTATCAACAAACAACTATGCACTTGATGAGATTGAAGAAGTTGCTTAA

mRNA sequence

ATGGCTGCCTCTAACCTTTCATGCATTGCTTCTGGGGTGGTCATTCCTTCTCCAACCACCATTTCATCCTCTTCTAGGTTATCTTCTGTCGCCTTTTTCCCCAAGAATTCCCACTCCCTTTCAAGATCATCCAGATTGGTTATCCGGGCCGCCGCCGAAGACGCTGCTCCCCCACCCCCTGCTGCCGCCGCCACCGCCGATGCCCCAGCCGAAGAGCCTAAGCCTAAGCCTGCCCCAATTGGCCCTAAGAGAGGTGCCAAGGTCAAGATTCTAAGGAGAGAGTCCTACTGGTACAAGGATGTTGGATCTGTTGTAACAGTTGACCAGGACCCAAACACTCGATATCCAGTTGTGGTTAGATTTAACAAAGTAAATTATGCAAGTGTATCAACAAACAACTATGCACTTGATGAGATTGAAGAAGTTGCTTAA

Coding sequence (CDS)

ATGGCTGCCTCTAACCTTTCATGCATTGCTTCTGGGGTGGTCATTCCTTCTCCAACCACCATTTCATCCTCTTCTAGGTTATCTTCTGTCGCCTTTTTCCCCAAGAATTCCCACTCCCTTTCAAGATCATCCAGATTGGTTATCCGGGCCGCCGCCGAAGACGCTGCTCCCCCACCCCCTGCTGCCGCCGCCACCGCCGATGCCCCAGCCGAAGAGCCTAAGCCTAAGCCTGCCCCAATTGGCCCTAAGAGAGGTGCCAAGGTCAAGATTCTAAGGAGAGAGTCCTACTGGTACAAGGATGTTGGATCTGTTGTAACAGTTGACCAGGACCCAAACACTCGATATCCAGTTGTGGTTAGATTTAACAAAGTAAATTATGCAAGTGTATCAACAAACAACTATGCACTTGATGAGATTGAAGAAGTTGCTTAA

Protein sequence

MAASNLSCIASGVVIPSPTTISSSSRLSSVAFFPKNSHSLSRSSRLVIRAAAEDAAPPPPAAAATADAPAEEPKPKPAPIGPKRGAKVKILRRESYWYKDVGSVVTVDQDPNTRYPVVVRFNKVNYASVSTNNYALDEIEEVA

Homology

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BLAST of Lag0005854 vs. NCBI nr
Match: XP_022937722.1 (photosystem I reaction center subunit IV, chloroplastic-like [Cucurbita moschata] >KAG6586174.1 Photosystem I reaction center subunit IV, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021006.1 Photosystem I reaction center subunit IV, chloroplastic [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 240.7 bits (613), Expect = 7.6e-60
Identity = 131/143 (91.61%), Postives = 136/143 (95.10%), Query Frame = 0


Query: 1   MAASNLSCIASGVVIPSPTTISSSSRLSSVAFFPKNSHSLSRSSRLVIRAAAEDAAPPPP 60
           MAASNLSCIASGV+IPS TTISSSSR SSVAFFPKNS S+SRS RLV+RAAA++AA PPP
Sbjct: 1   MAASNLSCIASGVIIPSSTTISSSSRPSSVAFFPKNSISISRSCRLVVRAAADEAAAPPP 60

Query: 61  AAAATADAPAEEPKPKPAPIGPKRGAKVKILRRESYWYKDVGSVVTVDQDPNTRYPVVVR 120
           AAAATADAPAE PKPKPAPIGPKRG KVKILRRESYWYKDVGSVVTVDQDPNTRYPVVVR
Sbjct: 61  AAAATADAPAEAPKPKPAPIGPKRGTKVKILRRESYWYKDVGSVVTVDQDPNTRYPVVVR 120

Query: 121 FNKVNYASVSTNNYALDEIEEVA 144
           FNKVNYASVSTNNYALDEIEEVA
Sbjct: 121 FNKVNYASVSTNNYALDEIEEVA 143

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BLAST of Lag0005854 vs. NCBI nr
Match: XP_022965997.1 (photosystem I reaction center subunit IV, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 238.0 bits (606), Expect = 4.9e-59
Identity = 130/143 (90.91%), Postives = 135/143 (94.41%), Query Frame = 0


Query: 1   MAASNLSCIASGVVIPSPTTISSSSRLSSVAFFPKNSHSLSRSSRLVIRAAAEDAAPPPP 60
           MAASNLSCIASGV+IPS  TISSSSR SSVAFFPKNS S+SRS RLV+RAAA++AA PPP
Sbjct: 1   MAASNLSCIASGVIIPSSPTISSSSRPSSVAFFPKNSISISRSCRLVVRAAADEAAAPPP 60

Query: 61  AAAATADAPAEEPKPKPAPIGPKRGAKVKILRRESYWYKDVGSVVTVDQDPNTRYPVVVR 120
           AAAATADAPAE PKPKPAPIGPKRG KVKILRRESYWYKDVGSVVTVDQDPNTRYPVVVR
Sbjct: 61  AAAATADAPAEVPKPKPAPIGPKRGTKVKILRRESYWYKDVGSVVTVDQDPNTRYPVVVR 120

Query: 121 FNKVNYASVSTNNYALDEIEEVA 144
           FNKVNYASVSTNNYALDEIEEVA
Sbjct: 121 FNKVNYASVSTNNYALDEIEEVA 143

[Close]

BLAST of Lag0005854 vs. NCBI nr
Match: XP_023536804.1 (photosystem I reaction center subunit IV, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 237.3 bits (604), Expect = 8.4e-59
Identity = 129/143 (90.21%), Postives = 135/143 (94.41%), Query Frame = 0


Query: 1   MAASNLSCIASGVVIPSPTTISSSSRLSSVAFFPKNSHSLSRSSRLVIRAAAEDAAPPPP 60
           MAASNLSCIASGV+IPS  TISSSSR SSVAFFPKNS S+SRS RLV+RAAA++AA PPP
Sbjct: 1   MAASNLSCIASGVIIPSSPTISSSSRPSSVAFFPKNSISISRSCRLVVRAAADEAAAPPP 60

Query: 61  AAAATADAPAEEPKPKPAPIGPKRGAKVKILRRESYWYKDVGSVVTVDQDPNTRYPVVVR 120
           AAAATADAPAE PKPKPAPIGPKRG KVKILR+ESYWYKDVGSVVTVDQDPNTRYPVVVR
Sbjct: 61  AAAATADAPAEAPKPKPAPIGPKRGTKVKILRKESYWYKDVGSVVTVDQDPNTRYPVVVR 120

Query: 121 FNKVNYASVSTNNYALDEIEEVA 144
           FNKVNYASVSTNNYALDEIEEVA
Sbjct: 121 FNKVNYASVSTNNYALDEIEEVA 143

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BLAST of Lag0005854 vs. NCBI nr
Match: XP_008441802.1 (PREDICTED: photosystem I reaction center subunit IV, chloroplastic-like [Cucumis melo])

HSP 1 Score: 233.0 bits (593), Expect = 1.6e-57
Identity = 131/144 (90.97%), Postives = 135/144 (93.75%), Query Frame = 0


Query: 1   MAASNLSCIASGVVIPSPTTI-SSSSRLSSVAFFPKNSHSLSRSSRLVIRAAAEDAAPPP 60
           MAASNLSCIASGVVIPS TTI SSSSRLSS+AFFPKNS+S+SRSSRLVIRAAA D A PP
Sbjct: 3   MAASNLSCIASGVVIPSSTTISSSSSRLSSLAFFPKNSNSISRSSRLVIRAAASDEAAPP 62

Query: 61  PAAAATADAPAEEPKPKPAPIGPKRGAKVKILRRESYWYKDVGSVVTVDQDPNTRYPVVV 120
           P AAA A A AEEPKPKPAPIGPKRGAKVKILR+ESYWYKDVGSVVTVDQDPNTRYPVVV
Sbjct: 63  PPAAAPAAATAEEPKPKPAPIGPKRGAKVKILRKESYWYKDVGSVVTVDQDPNTRYPVVV 122

Query: 121 RFNKVNYASVSTNNYALDEIEEVA 144
           RFNKVNYASVSTNNYALDEIEEVA
Sbjct: 123 RFNKVNYASVSTNNYALDEIEEVA 146

[Close]

BLAST of Lag0005854 vs. NCBI nr
Match: XP_022144405.1 (photosystem I reaction center subunit IV, chloroplastic-like [Momordica charantia])

HSP 1 Score: 232.6 bits (592), Expect = 2.1e-57
Identity = 127/143 (88.81%), Postives = 135/143 (94.41%), Query Frame = 0


Query: 1   MAASNLSCIASGVVIPSPTTISSSSRLSSVAFFPKNSHSLSRSSRLVIRAAAEDAAPPPP 60
           MAA+NLSCIASGVVIPS  TISSSSRL+SVAFFPKN + +SR  RLVIRAAA++AAPPPP
Sbjct: 1   MAATNLSCIASGVVIPSSATISSSSRLTSVAFFPKNPNPISR--RLVIRAAADEAAPPPP 60

Query: 61  AAAATADAPAEEPKPKPAPIGPKRGAKVKILRRESYWYKDVGSVVTVDQDPNTRYPVVVR 120
           AAAATADAP EE KPKPAPIGPKRGAKVKILR+ESYWYKDVGSVVTVDQDPNTRYPVVVR
Sbjct: 61  AAAATADAPVEEAKPKPAPIGPKRGAKVKILRKESYWYKDVGSVVTVDQDPNTRYPVVVR 120

Query: 121 FNKVNYASVSTNNYALDEIEEVA 144
           FNKVNYASVSTNNYALDE+EEVA
Sbjct: 121 FNKVNYASVSTNNYALDEVEEVA 141

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BLAST of Lag0005854 vs. ExPASy Swiss-Prot
Match: Q41229 (Photosystem I reaction center subunit IV B, chloroplastic OS=Nicotiana sylvestris OX=4096 GN=PSAEB PE=1 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 4.3e-34
Identity = 86/145 (59.31%), Postives = 108/145 (74.48%), Query Frame = 0


Query: 1   MAASNLSCIASGVVIPSP--TTISSSSRLSSVAFFPKNSHSLSRSSRLVIRAAAEDAAPP 60
           MA+S+++  ASG ++ +P   T +++ R S + F    +++ +   RLV+RAA E A   
Sbjct: 1   MASSSMASAASGFMVATPNIATSNTAPRTSMLFFSSSKNNTTTNFPRLVVRAAEEAA--- 60

Query: 61  PPAAAATADAPAEEPK-PKPAPIGPKRGAKVKILRRESYWYKDVGSVVTVDQDPNTRYPV 120
           PPAA ATA+  A   K  KP PIGPKRG KV++LR+ESYWYK VGSVV VDQDPNTRYPV
Sbjct: 61  PPAATATAEGEAPPAKAAKPPPIGPKRGTKVRVLRKESYWYKGVGSVVAVDQDPNTRYPV 120

Query: 121 VVRFNKVNYASVSTNNYALDEIEEV 143
           VVRFNKVNYA+VSTNNYALDE+EEV
Sbjct: 121 VVRFNKVNYANVSTNNYALDEVEEV 142

[Close]

BLAST of Lag0005854 vs. ExPASy Swiss-Prot
Match: Q41228 (Photosystem I reaction center subunit IV A, chloroplastic OS=Nicotiana sylvestris OX=4096 GN=PSAEA PE=1 SV=1)

HSP 1 Score: 144.4 bits (363), Expect = 9.7e-34
Identity = 87/144 (60.42%), Postives = 105/144 (72.92%), Query Frame = 0


Query: 1   MAASNLSCIASGVVIPSPTTISSSSRLSSVAFFPKNSHSLSRSSRLVIRAAAEDAAPPPP 60
           MA+ N++  AS  ++ +P   S+++   +   F  + +  S + RLV+RAA E A   PP
Sbjct: 1   MASCNMASAASNFLVATPNVASNTNTSRTTMLFFSSKNYGSTAPRLVVRAAEEAA---PP 60

Query: 61  AAAATADAPAEEP--KPKPAPIGPKRGAKVKILRRESYWYKDVGSVVTVDQDPNTRYPVV 120
           AAAATA+ PAE P    KP PIGPKRG KV+ILR+ESYWYK  GSVV  DQDPNTRYPVV
Sbjct: 61  AAAATAE-PAEAPVKAAKPPPIGPKRGTKVRILRKESYWYKGTGSVVACDQDPNTRYPVV 120

Query: 121 VRFNKVNYASVSTNNYALDEIEEV 143
           VRFNKVNYA+VSTNNYALDEIEEV
Sbjct: 121 VRFNKVNYANVSTNNYALDEIEEV 140

[Close]

BLAST of Lag0005854 vs. ExPASy Swiss-Prot
Match: P12354 (Photosystem I reaction center subunit IV, chloroplastic OS=Spinacia oleracea OX=3562 GN=PSAE-1 PE=1 SV=1)

HSP 1 Score: 139.0 bits (349), Expect = 4.1e-32
Identity = 79/122 (64.75%), Postives = 92/122 (75.41%), Query Frame = 0


Query: 22  SSSSRLSSVAFFPKNSHSLSRSSRLVIRAAAEDAAPPPPAAAATADAPAEEPKPKPAPIG 81
           S + RL      P  + S  RS+RLV+RAA E AA P   AAA+ +  A +   KP PIG
Sbjct: 7   SVAVRLGLTQVLPNKNFSSPRSTRLVVRAAEEAAAAP---AAASPEGEAPKAAAKPPPIG 66

Query: 82  PKRGAKVKILRRESYWYKDVGSVVTVDQDPNTRYPVVVRFNKVNYASVSTNNYALDEIEE 141
           PKRG+KV+I+R+ESYWYK VGSVV VDQDP TRYPVVVRFNKVNYA+VSTNNYALDEI+E
Sbjct: 67  PKRGSKVRIMRKESYWYKGVGSVVAVDQDPKTRYPVVVRFNKVNYANVSTNNYALDEIQE 125

Query: 142 VA 144
           VA
Sbjct: 127 VA 125

[Close]

BLAST of Lag0005854 vs. ExPASy Swiss-Prot
Match: Q9S831 (Photosystem I reaction center subunit IV A, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PSAE1 PE=1 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 6.9e-32
Identity = 88/138 (63.77%), Postives = 100/138 (72.46%), Query Frame = 0


Query: 14  VIPSPTT--ISSSSRLSSVAFFPKNSHSLSRSSRLVIRAAAEDAAP------PPPAAAAT 73
           V+P+  T    +SS  SSV+F P      +  SRLV+R AAED AP        PAAAA 
Sbjct: 11  VLPANVTSVAGASSSRSSVSFLPMR----NAGSRLVVR-AAEDPAPASSSSKDSPAAAAA 70

Query: 74  ADAPAEEPKPKPAPIGPKRGAKVKILRRESYWYKDVGSVVTVDQDPNTRYPVVVRFNKVN 133
            D  A   KPKP PIGPKRG+KVKILRRESYW+K+VGSVV VDQDP TRYPVVVRF KVN
Sbjct: 71  PDG-ATATKPKPPPIGPKRGSKVKILRRESYWFKNVGSVVAVDQDPKTRYPVVVRFAKVN 130

Query: 134 YASVSTNNYALDEIEEVA 144
           YA++STNNYALDE+EEVA
Sbjct: 131 YANISTNNYALDEVEEVA 142

[Close]

BLAST of Lag0005854 vs. ExPASy Swiss-Prot
Match: Q9S714 (Photosystem I reaction center subunit IV B, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PSAE2 PE=2 SV=1)

HSP 1 Score: 129.8 bits (325), Expect = 2.5e-29
Identity = 81/137 (59.12%), Postives = 96/137 (70.07%), Query Frame = 0


Query: 15  IPSPTTISSSSRLSSVAFFPKNSHSLSRSSRLVIRAAAEDAAPPPPAAAAT-------AD 74
           +P+     SS   + V+F P  S      SRLV+RAA +     PPA A++       A 
Sbjct: 16  VPAAIGGGSSKSTTIVSFLPMRSF----GSRLVVRAAED----TPPATASSDSSSTTAAA 75

Query: 75  APAEEP--KPKPAPIGPKRGAKVKILRRESYWYKDVGSVVTVDQDPNTRYPVVVRFNKVN 134
           APA+ P  K KP PIGPKRG+KVKILR+ESYWYK+VGSVV VDQDP TRYPVVVRF KVN
Sbjct: 76  APAKVPAAKAKPPPIGPKRGSKVKILRKESYWYKNVGSVVAVDQDPKTRYPVVVRFAKVN 135

Query: 135 YASVSTNNYALDEIEEV 143
           YA++STNNYALDE+EEV
Sbjct: 136 YANISTNNYALDEVEEV 144

The following BLAST results are available for this feature:

BLAST of Lag0005854 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022937722.1	7.6e-60	91.61	photosystem I reaction center subunit IV, chloroplastic-like [Cucurbita moschata...	[more]
XP_022965997.1	4.9e-59	90.91	photosystem I reaction center subunit IV, chloroplastic-like [Cucurbita maxima]	[more]
XP_023536804.1	8.4e-59	90.21	photosystem I reaction center subunit IV, chloroplastic-like [Cucurbita pepo sub...	[more]
XP_008441802.1	1.6e-57	90.97	PREDICTED: photosystem I reaction center subunit IV, chloroplastic-like [Cucumis...	[more]
XP_022144405.1	2.1e-57	88.81	photosystem I reaction center subunit IV, chloroplastic-like [Momordica charanti...	[more]

BLAST of Lag0005854 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q41229	4.3e-34	59.31	Photosystem I reaction center subunit IV B, chloroplastic OS=Nicotiana sylvestri...	[more]
Q41228	9.7e-34	60.42	Photosystem I reaction center subunit IV A, chloroplastic OS=Nicotiana sylvestri...	[more]
P12354	4.1e-32	64.75	Photosystem I reaction center subunit IV, chloroplastic OS=Spinacia oleracea OX=...	[more]
Q9S831	6.9e-32	63.77	Photosystem I reaction center subunit IV A, chloroplastic OS=Arabidopsis thalian...	[more]
Q9S714	2.5e-29	59.12	Photosystem I reaction center subunit IV B, chloroplastic OS=Arabidopsis thalian...	[more]

InterPro

Analysis Name: InterPro Annotations of Sponge gourd (AG-4) v1
Date Performed: 2022-08-01


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003375	Photosystem I PsaE, reaction centre subunit IV	PFAM	PF02427	PSI_PsaE	coord: 83..142 e-value: 3.0E-30 score: 103.7
IPR003375	Photosystem I PsaE, reaction centre subunit IV	PANTHER	PTHR34549	PHOTOSYSTEM I REACTION CENTER SUBUNIT IV A, CHLOROPLASTIC-RELATED	coord: 1..143
None	No IPR available	GENE3D	2.30.30.50		coord: 76..143 e-value: 3.2E-36 score: 124.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 53..85
None	No IPR available	PANTHER	PTHR34549:SF7	PHOTOSYSTEM I REACTION CENTER SUBUNIT IV A	coord: 1..143
IPR008990	Electron transport accessory-like domain superfamily	SUPERFAMILY	50090	Electron transport accessory proteins	coord: 82..142

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Lag0005854.1	Lag0005854.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0015979	photosynthesis
cellular_component	GO:0009535	chloroplast thylakoid membrane
cellular_component	GO:0009538	photosystem I reaction center
cellular_component	GO:0009522	photosystem I